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Infestation of mice with ixodid ticks in forests of Belgorod region 别尔哥罗德地区森林中蜱虫对老鼠的侵害
Pub Date : 2022-12-27 DOI: 10.22363/2312-797x-2022-17-4-536-545
I. Glamazdin, A. Tkachev, O. Tkacheva, E. Krotova, S. Drukovskiy, A. Petrov
Rodents are one of the largest groups of existing mammals and number more than 2,270 known species, which is almost 42 % of the total biodiversity of worldwide-distributed mammals (except Antarctica and some islands). They are well adapted to various habitats and are known to live alongside people and livestock complexes. The purpose of the study was to study infestation of mice with ixodid ticks in forests of the Belgorod region adjacent to livestock and poultry farms. The study was carried out in 2019-2022. The extensiveness of mice infestation was observed in Sylvaemus sylvaticus species, which was 13.26 % higher (P 0.05) than in Sylvimus flavicollis , and 24.33 % higher (P 0.01) than in Apodemus agrarius . The intensity of infestation with preimaginal (larvae and nymphs) stages of Ixodes ricinus ticks had wide fluctuations depending on the type of synanthropic rodent. Thus, the highest intensity was noted in Sylvaemus sylvaticus mice, which was 8.73 and 13.56 % higher (P 0.001) compared to Sylvimus flavicollis and Apodemus agrarius mice, respectively. The maximum infestation of male synanthropic mouse species was 31 ticks in Sylvaemus sylvaticus , 19 ticks in Sylvimus flavicollis , and 9 ticks in Apodemus agrarius . The intensity of infestation in synanthropic mice was the highest in the summer months (from July to August) and decreased slightly in autumn. In autumn, 41.2 % of the examined rodents were infested with ticks, the predominant species was Ixodes ricinus (larvae). Larvae and nymphs of Ixodes ricinus ticks were more often attached to rodents in the head area (mainly on the ears) - 82 %, less often on other parts of the body - 1 8 %. A smaller number of ticks were present on neck, body, legs, sometimes parasites were found on tail.
啮齿动物是现存最大的哺乳动物群体之一,已知的种类超过2270种,几乎占全世界分布的哺乳动物生物多样性总量的42%(南极洲和一些岛屿除外)。它们很好地适应了各种栖息地,并与人类和牲畜一起生活。本研究的目的是研究别尔哥罗德地区牲畜和家禽养殖场附近森林中蜱虫对老鼠的侵害。该研究于2019年至2022年进行。结果表明,森林木鼠的鼠患广泛性比黄木鼠高13.26% (p0.05),比黑线姬鼠高24.33% (p0.01)。蓖麻硬蜱幼虫和若虫的侵袭强度随鼠种的不同而有较大的波动。结果表明,与黄缕鼠和黑线姬鼠相比,山楂鼠的辐射强度最高,分别高出8.73%和13.56% (P < 0.001)。雄性共交鼠种最多侵染的是森林小檗、黄小檗和黑线姬鼠,分别为31、19和9只。夏季(7 ~ 8月)鼠患强度最高,秋季略有下降。秋季调查鼠类中,41.2%为蜱虫,优势种为蓖麻伊蚊(幼虫)。蓖麻蜱的幼虫和若虫更多地附着在啮齿动物的头部(主要在耳朵上)(82%),较少地附着在身体其他部位(18%)。颈部、身体、腿部有少量蜱虫,尾部有时发现寄生虫。
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引用次数: 0
Reproductive capacity and milk production of cows depending on their physio-logical status during lactation 奶牛的繁殖能力和产奶量取决于其泌乳期的生理状态
Pub Date : 2022-12-27 DOI: 10.22363/2312-797x-2022-17-4-484-498
Maria V. Kornelaeva, G. Karlikova
Due to violation of husbandry, feeding, milking, non-observance of sanitary requirements, especially in postpartum period, cases of endometritis, mastitis and limb diseases in cows increase in conditions of intensive cattle breeding. These diseases adversely affect reproductive and productive qualities of animals, thus causing great financial losses to agribusiness. Therefore, increasing longevity of animals, reducing percentage of their elimination due to various diseases, reducing risk of economically significant diseases in dairy herds by selecting highly productive animals with high resistance to common diseases are relevant. The aim of the research was to study phenotypic relationship between fertility level and milk production of black-motley Holsteinized cows with different diseases at the farm in Moscow region. The research materials were 1234 records of diseases - endometritis, mastitis and limb diseases - obtained from veterinary outpatient logs (2015-2021) of the breeding organization. Information about 5 signs of milk productivity and 3 signs of fertility of the studied population of black-motley Holsteinized dairy cattle was taken from Seleks database. According to the results, significant (p 0.01) increase was observed in sick animals in comparison with healthy ones: number of inseminations per lactation - b y 33.5 %, open days - b y 7.3 %, milk days - b y 4.35 %. Milk yield was higher by 19.4 % (p 0.01), fat and protein mass fraction - by 0.32 and 0.33 % (p 0.01), respectively, fat and protein yield - b y 82 and 69 kg (p 0.01), respectively, compared to healthy animals. When comparing the indices in animals with one, two and three diseases, significant differences were obtained both between the groups with single diseases and complexes. For fertility traits, the indices increased significantly with the number of diseases from 4 to 20 % for individual traits. For milk production traits, there was a tendency for indices to decrease with increasing number of diseases.
由于违反放牧、饲养、挤奶、不遵守卫生要求,特别是在产后,奶牛子宫内膜炎、乳腺炎和肢体疾病的病例在集约化养殖条件下增加。这些疾病对动物的繁殖和生产品质产生不利影响,从而给农业企业造成巨大的经济损失。因此,通过选择对常见疾病具有高抵抗力的高产动物,增加动物寿命,减少因各种疾病而被消灭的百分比,降低奶牛群中经济上重大疾病的风险是相关的。本研究的目的是研究莫斯科地区农场不同疾病黑斑荷斯坦奶牛的生育水平与产奶量之间的表型关系。研究资料来源于养殖单位2015-2021年兽医门诊日志1234例疾病记录,包括子宫内膜炎、乳腺炎和肢体疾病。从Seleks数据库中获取了黑斑荷斯坦奶牛种群的5个产乳指标和3个育乳指标。结果表明,病畜每次泌乳受精卵数增加33.5%,开工日增加7.3%,产奶日增加4.35%,显著高于健康畜(p < 0.01)。与健康动物相比,产奶量提高19.4% (p 0.01),脂肪和蛋白质质量分数分别提高0.32%和0.33% (p 0.01),脂肪和蛋白质产量分别提高82和69 kg (p 0.01)。在比较一种、二种和三种疾病动物的各项指标时,单一疾病组和复合疾病组之间均有显著差异。在育性性状上,各指标随单个性状的病率从4%增加到20%而显著增加。产奶量性状指标随病害数量的增加呈下降趋势。
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引用次数: 0
Identification of Pseudomonas fuscovaginae, Pseudomonas syringae and Xanthomonas translucens in wheat seeds using PCR 小麦种子中褐阴道假单胞菌、丁香假单胞菌和透光黄单胞菌的PCR鉴定
Pub Date : 2022-12-27 DOI: 10.22363/2312-797x-2022-17-4-473-483
M. Muvingi, O. Slovareva, M. Zargar
The causative agents of grain crops bacteriosis viz. Pseudomonas fuscovaginae , Pseudomonas syringae and Xanthomonas translucens are regulated by phytosanitary requirements of the largest importers of Russian grain - Egypt, Turkey, Bangladesh, Nigeria and Pakistan. Therefore, it requires the development of rapid methods for their diagnosis. The PCR method, which is the fastest and most reliable in testing laboratories, needs optimal preparation of the test material. The aim of the study was to optimize the process of preparing seed samples for subsequent detection and identification of P. fuscovaginae, P. syringae and X. translucens by PCR. Wheat grain samples were soaked in phosphate-buffered saline (PBS) for 2 hours and infected with suspensions of P. fuscovaginae, P. syringae pv. coronafaciens and X. translucens at various concentrations. Then, the infected grain samples were crushed and subjected to two-stage centrifugation. DNA was isolated from the obtained analytical samples and species-specific PCR was performed for each bacterial species. It was found that a two-hour soaking of the seeds and their treatment with a homogenizer is sufficient to effectively destroy each grain in the sample and ensure the release of bacteria into the liquid part of the sample. The first low-speed centrifugation allowed the crushed grain to settle efficiently and remove excess starch from the supernatant. High-speed centrifugation of the supernatant made it possible to obtain a concentrated microbiota contained in the grain sample. To obtain DNA of sufficient quality for PCR test, the kit Proba-GS (AgroDiagnostika, Russia) was used for DNA extraction. Using Pseudomonas fuscovaginae-RT kit (Syntol, Russia) and PsyF/PsyR and 4F1/4R 1 primers, DNA of P. fuscovaginae P. syringae and X. translucens , respectively, was successfully detected in each of the samples infected with these bacteria at concentrations of 103 CFU/ml. The absence of PCR inhibition was noted. The method of removing starch from samples for molecular diagnostics of phytopathogens was used for the first time. Application of these methods will allow diagnosing pathogens of bacterioses within one day.
粮食作物细菌病的病原体,即褐藻假单胞菌、丁香假单胞菌和半透明黄单胞菌,受俄罗斯最大粮食进口国埃及、土耳其、孟加拉国、尼日利亚和巴基斯坦的植物检疫要求的规管。因此,需要开发快速诊断方法。PCR方法是检测实验室中最快、最可靠的方法,需要对检测材料进行最佳制备。本研究的目的是优化种子样品的制备过程,以便随后通过PCR检测和鉴定黄颡菌、丁香和半透明X。小麦颗粒样品在磷酸盐缓冲盐水(PBS)中浸泡2小时,并用P.fuscovaginae、P.syringae pv的悬浮液感染。coronafaciens和X.半透明。然后,将受感染的谷物样品粉碎并进行两阶段离心。从获得的分析样品中分离DNA,并对每个细菌物种进行物种特异性PCR。研究发现,种子浸泡两小时并用均质器处理就足以有效地破坏样品中的每一粒颗粒,并确保细菌释放到样品的液体部分。第一次低速离心使粉碎的颗粒有效沉降,并从上清液中去除多余的淀粉。上清液的高速离心可以获得谷物样品中所含的浓缩微生物群。为了获得用于PCR检测的足够质量的DNA,使用试剂盒Proba GS(AgroDiagnostika,Russia)进行DNA提取。使用褐藻假单胞菌RT试剂盒(Syntol,Russia)和PsyF/PsyR和4F1/4R1引物,在感染这些细菌的每个样本中分别成功检测到褐藻假单孢菌、丁香假单胞菌和半透明假单胞菌的DNA,浓度为103CFU/ml。注意到没有PCR抑制。从样品中去除淀粉的方法首次用于植物病原体的分子诊断。这些方法的应用将允许在一天内诊断细菌的病原体。
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引用次数: 1
Breeding characteristics of Romanov sheep depending on polymorphism of growth hormone gene 生长激素基因多态性对罗曼诺夫羊育种特性的影响
Pub Date : 2022-12-27 DOI: 10.22363/2312-797x-2022-17-4-514-526
M. Abramova, A. V. Ilina, M. S. Barysheva, Julia I. Malina, E. Evdokimov
The search for new markers of high productivity of sheep and the introduction of evaluation and selection methods for these indicators is an urgent direction in breeding of Russian sheep. The material for the research was the results of assessment of exterior, live weight of Romanov sheep. Genetic studies were carried out using tissue samples (ear plucking). Polymorphism of growth hormone gene in Romanov sheep was evaluated by restriction fragment length polymorphism analysis and polymerase chain reaction. Evaluation of polymorphism of growth hormone gene in the population of Romanov sheep showed the presence of three allelic variants - AA, AB and BB, with a frequency of 0.24, 0.63 and 0.14, respectively. The statistically significant difference between the indicators of live weight in animals with genotypes GHAB and GHAA, GHAB and GHBB was: at birth 210 g and 140 g (P 0.95), respectively; at weaning 350 g and 1260 g (P 0.95), respectively. The phenotypic variability of live weight was the highest at birth and at weaning and amounted to 31.732.5 % for all genotypes. Animals with genotypes GHAA and GHAB outperformed their peers with genotype GHBB in dimensions of rump width in tuber coxae, rump width in tuber trochanterica, chest width, chest circumference, and therefore, had a wide body. When studying the phenotypic and genetic correlations of measurements of exterior and live weight at the age of 12 months, a reliable positive relationship was established with height at withers height (rP = +0.13*; rG = +0.02), rump height (rP = +0.14**; rG = +0.03), rump length (rP = +0.14**; rG = +0.02). The new knowledge gained about polymorphism of GH gene and its relationship with productive traits of Romanov sheep will allow for in-depth evaluation, targeted selection and selection of individuals with desirable genotypes.
寻找新的绵羊高产标记并引入这些指标的评价和选择方法是俄罗斯绵羊育种的一个紧迫方向。研究材料是对罗曼诺夫绵羊的外表和活重进行评估的结果。使用组织样本(拔耳)进行遗传研究。采用限制性片段长度多态性分析和聚合酶链式反应对罗曼诺夫绵羊生长激素基因的多态性进行了评价。对罗曼诺夫绵羊群体生长激素基因多态性的评估显示,存在三个等位基因变体——AA、AB和BB,频率分别为0.24、0.63和0.14。GHAB和GHAA、GHAB和GH BB基因型动物的活重指标之间的统计学显著差异分别为:出生时210克和140克(P 0.95);断奶时分别为350g和1260g(P 0.95)。活重在出生和断奶时的表型变异性最高,所有基因型的表型变异率为31.732.5%。GHAA和GHAB基因型的动物在髋结节臀宽、粗隆结节臀宽和胸宽、胸围方面优于GHBB基因型的同类动物,因此,它们的身体很宽。当研究12个月大时外部和活重测量的表型和遗传相关性时,与肩颈高度(rP=+0.13*;rG=+0.02)、臀部高度(rP=+0.14**;rG=+0.03)、,臀长(rP=+0.14**;rG=+0.02)。对罗曼诺夫绵羊GH基因多态性及其与生产性状关系的新认识将有助于深入评估、有针对性地选择和筛选具有理想基因型的个体。
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引用次数: 0
Morphological characteristics of testicular interstitial cell tumors in dogs 犬睾丸间质细胞瘤的形态学特征
Pub Date : 2022-12-27 DOI: 10.22363/2312-797x-2022-17-4-527-535
A. Gazin, Y. Vatnikov, E. Abramova
The study presents an assessment of variability of histological structure, measurement, and comparison of size of the neoplasm obtained by ultrasonographic examination and pathological examination, as well as the morphometric dimensions of nuclei and cytoplasm of testicular interstitial cell tumors in dogs. The study involved 35 dogs with neoplasms of 46 testes, where 11 animals had interstitial cell tumors in both testes. Insignificant differences of the size of these neoplasms were revealed (p 0.05) using ultrasonography and pathoanatomical measurement methods. Hence, it allows using both methods to assess the size of interstitial cell tumors. In the study, interstitial cell tumor was detected in both testes at once in 50 % of cases in dogs, however, this might be due to specific characteristics of the sample, and further research is required. In the course of scientific work, a morphological study showed the presence of variability in histological structure of interstitial cell tumors, which can lead to incorrect interpretation of the morphological picture and misdiagnosis, e. g. adipocyte-like morphology of interstitial cell tumors have morphological similarity to a benign neoplasm from adipose tissue - lipoma. In addition, there was an extremely pronounced difference in size of cytoplasm (from 23.6 to 148.4 m; average 66.21 22.42 m) and nuclei (from 9 to 57.6 m; average 23.19 7.10 m) in tumor cells. It proves the presence of pronounced anisocytosis and anisokariosis, which should indicate malignancy of the neoplasm, however, testicular interstitial cell tumors extremely rarely metastasize in practice and according to numerous studies.
本研究对犬睾丸间质细胞瘤的组织学结构变异性进行了评估,并对超声检查和病理检查所得肿瘤的大小进行了测量和比较,并对其细胞核和细胞质的形态尺寸进行了测量。这项研究涉及35只患有46个睾丸肿瘤的狗,其中11只动物的两个睾丸都有间质细胞肿瘤。超声及病理解剖测量结果显示肿瘤大小差异无统计学意义(p < 0.05)。因此,它允许使用这两种方法来评估间质细胞肿瘤的大小。在这项研究中,在50%的狗的病例中,同时在两个睾丸中检测到间质细胞肿瘤,然而,这可能是由于样本的特定特征,需要进一步的研究。在科学工作过程中,形态学研究发现间质细胞瘤的组织学结构存在变异性,这可能导致对形态学图像的错误解释和误诊,如间质细胞瘤的脂肪细胞样形态与脂肪组织的良性肿瘤-脂肪瘤形态相似。此外,细胞质的大小也有极显著的差异(从23.6到148.4 m;平均66.21 ~ 22.42 m)和核(9 ~ 57.6 m;平均23.19 7.10 m)。结果表明,睾丸间质细胞瘤存在明显的异细胞增多和异细胞增多,应提示肿瘤为恶性肿瘤,然而,在实践和大量研究中,睾丸间质细胞瘤极少发生转移。
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引用次数: 0
Promising varieties of sour cherry Prunus cerasus L. with a complex of fruit quality traits for growing in the southern Russia 具有复杂果实品质特征的有前途的酸樱桃品种,适合在俄罗斯南部种植
Pub Date : 2022-12-27 DOI: 10.22363/2312-797x-2022-17-4-437-447
R. Zaremuk, T. Kopnina
Southern assortment of cherries does not quite meet the modern requirements of production. Many varieties are low-productive, not resistant to cherry leaf spot ( Coccomyces hiemalis ) and moniliosis ( Monilia cinerea ), small-fruited, with low taste qualities. However, new varieties have been created that have not been studied by the complex of consumer and commodity qualities of fruits. Thus, evaluation of new varieties for these traits is relevant. The aim of the research was to evaluate cherry varieties of different origin for commodity, biochemical, and consumer qualities of fruits. The research was carried out in the Kuban horticultural zone of the Krasnodar territory. Objects of the research were 9 varieties of sour cherry trees. Field and laboratory studies were conducted according to Program and methodology of varietal study of fruit, berry and nut crops (1999), Methodological instructions on chemical and technological variety testing of vegetable, fruit and berry crops for canning industry (1993). Statistical analysis was carried out according to B.A. Dospekhov (2014) and G.F. Lakin (1990). It was found that the average fruit weight of cherry varieties varied not significantly - from 2.72 to 6.45 g, which was confirmed by the coefficient of variation (23.3 %). Indicators of maximum and minimum fruit weight varied significantly in varieties, coefficients of variation were 27.2 and 29.7 %, respectively. The following cherry varieties with large fruits were identified: Timati, Igrushka, Duk Ivanovna, Duk Khodosa, Prizvaniya and Svetlaya. were distinguished. Cherry varieties with high sugar content in fruits were as follows: Feyа, Dzhusi Frut and Igrushka. Feyа, Duk Khodosa, Prizvanie, Svetlaya and Dzhusi Frut had fruits with low acidity; Feyа, Dzhusi Frut and Igrushka fruits were characterized by high content of soluble solids in fruits. Feyа, Igrushka and Duk Khodosa fruits had the highest content of vitamin C; Igrushka, Duk Khodosa, Prizvanie, Svetlaya and Dzhusi Frut were rich in Vitamin P; Duk Khodosa, Prizvanie, Dzhusi Frut and Duk Ivanovna had the largest anthocyanin content. Therefore, Igrushka and Duk Khodosa cherry varieties are recommended for growing in the southern Russia and breeding for improvement of fruit quality.
南方的樱桃品种不太符合现代生产的要求。许多品种产量低,不抗樱桃叶斑病(Coccomyces hiemalis)和念珠菌病(Monilia cinerea),果实小,味道差。然而,新品种已经被创造出来,但这些新品种还没有经过水果消费和商品质量的综合研究。因此,评价这些性状的新品种是有意义的。本研究的目的是评价不同产地樱桃品种的商品品质、生化品质和消费品质。这项研究是在克拉斯诺达尔地区的库班园艺区进行的。以9个酸樱桃品种为研究对象。根据水果、浆果和坚果作物品种研究计划和方法(1999年)、罐头工业蔬菜、水果和浆果作物化学和技术品种试验方法说明(1993年)进行了实地和实验室研究。根据B.A. Dospekhov(2014)和G.F. Lakin(1990)进行统计分析。结果表明,各樱桃品种的平均单果重变化不显著,在2.72 ~ 6.45 g之间,变异系数为23.3%。品种间最大、最小单果重指标差异显著,变异系数分别为27.2%和29.7%。确定了以下果实较大的樱桃品种:Timati、Igrushka、Duk Ivanovna、Duk Khodosa、Prizvaniya和Svetlaya。是杰出的。果实含糖量较高的樱桃品种有:飞亚、珠斯和伊格鲁什卡。feyfa、Duk Khodosa、Prizvanie、Svetlaya和Dzhusi fruit的果实酸度较低;飞果、朱斯果和伊格鲁什卡果的果实中可溶性固形物含量较高。feyafe、Igrushka和Duk Khodosa水果的维生素C含量最高;Igrushka果、Duk Khodosa果、Prizvanie果、Svetlaya果和Dzhusi果富含维生素P;杜克·霍多萨、普里兹瓦尼、朱斯果和杜克·伊万诺夫果的花青素含量最高。因此,Igrushka和Duk Khodosa樱桃品种被推荐在俄罗斯南部种植和育种,以改善果实品质。
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引用次数: 0
Use of Chlorella vulgaris as a dietary supplement for quails bred at private farms 使用小球藻作为私人农场饲养鹌鹑的膳食补充剂
Pub Date : 2022-12-27 DOI: 10.22363/2312-797x-2022-17-4-499-513
L. Medvedeva, Ol’ga V. Zorkina, M. Moskovets
Some aspects of improving efficiency of quail breeding in Russia, in particular in private farms were studied. It is emphasized that in times of crisis, private farms are able to solve several problems: provide employment for rural residents, produce food, and serve as a source of additional income for people. In Russia, consumption of animal protein in poultry meat has increased to 34 kg. The aim of the research was to study the issues of breeding quails on small plots, at private farms; the use of Chlorella vulgaris microalgae in the diet of poultry, which has become intensively used in various sectors of the economy, in particular, in animal husbandry and improvement of natural reservoirs. Methods of analysis, observation, experiment, comparison were used in the course of the study; photographing and timing of resources spent on the maintenance of quail birds was carried out. The Russian and foreign scientific literature was studied, the presented material was compared with the results obtained during the experiment. One aspect of successful quail breeding is to ensure a balanced diet - composition of feeds produced by local producers for quails was studied. The observations and experiments carried out on Moscow White Giant quails at E. Moskovets private farm (Volgograd region) proved the expediency of introducing into the diet a feed additive - a strain of Chlorella vulgaris IFR C.111, which contains protein, carbohydrates, fats, vitamins, mineral salts, trace elements in sufficient quantities. The technology of poultry feeding with Chlorella vulgaris was shown. It resulted in higher survival rate of quails, increase in poultry live weight, and increase in profitability of production by 1.3 %. Data reflecting the conditions of quail keeping, feeding and cage breeding in small areas and/or private farms were shown.
研究了提高俄罗斯鹌鹑养殖效率的一些方面,特别是在私人农场。有人强调,在危机时期,私人农场能够解决几个问题:为农村居民提供就业机会,生产粮食,并成为人们的额外收入来源。在俄罗斯,禽肉中动物蛋白的消耗量已增加到34公斤。研究的目的是研究在私人农场的小块土地上饲养鹌鹑的问题;小球藻微藻在家禽饲料中的应用,已广泛应用于各个经济部门,特别是畜牧业和天然水库的改善。研究采用分析、观察、实验、比较等方法;对鹌鹑鸟的养护所花费的资源进行了拍照和计时。对俄罗斯和国外的科学文献进行了研究,并将所提供的材料与实验结果进行了比较。成功饲养鹌鹑的一个方面是确保均衡的饮食——研究了当地生产商为鹌鹑生产的饲料组成。在E.Moskovets私人农场(伏尔加格勒地区)对莫斯科白巨鹌鹑进行的观察和实验证明了在饮食中添加饲料添加剂的方便性——一种小球藻IFR C.111菌株,它含有足够数量的蛋白质、碳水化合物、脂肪、维生素、矿物盐和微量元素。介绍了用小球藻饲养家禽的技术。它提高了鹌鹑的存活率,增加了家禽的活重,使生产利润率提高了1.3%。显示了反映小面积和/或私人农场鹌鹑饲养、饲养和笼养条件的数据。
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引用次数: 0
Effect of biofertilizers and osmotic stress on morphological parameters of spring barley seedlings 生物肥料和渗透胁迫对春大麦幼苗形态参数的影响
Pub Date : 2022-12-27 DOI: 10.22363/2312-797x-2022-17-4-425-436
Alexander L Panfilov, Rinat R. Abdrashitov
One of ways to increase resistance of agricultural plants to abiotic stresses is the use of biofertilizers with anti-stress and growth-regulating properties. They improve absorption of nitrogen and phosphorus from organic fertilizers and soil. The purpose of the research was to study sowing qualities of seeds, morphological indicators of spring barley seedlings after presowing seed treatment with biofertilizers under conditions of sufficient humidification and osmotic stress. The laboratory experiment was carried out to study the effect of biofertilizers on seedlings of spring barley ( Hordeum vulgare L.) cv. Gubernatorsky under conditions of sufficient and insufficient humidification. The barley seeds were treated once according to the following scheme: 1. control (distilled water) 10 L/t; 2. Gumi 20 M potash (0.4 L/t); 3. Borogum-M complex (0.2 L/t); 4. PEG 6000 (100 g/L); 5. Gumi 20 M (0.4 L/t) + PEG 6000 (100 g/L); 6. Borogum-M complex (0.2 L/t) + PEG 6000 (100 g/L). Treatment of spring barley seeds with biofertilizers increased the germination rate and germination capacity by 35 and 2 %, respectively. When modeling drought using PEG-6000, the sowing qualities of seeds decreased by 4 %. Under conditions of sufficient humidification, the studied biofertilizers had a complex positive effect on shoot and root weight of barley seedlings. In addition, the average length of roots, shoots and the total root length increased. The number of roots did not change significantly. Lack of moisture decreased the effectiveness of Borogum-M complex biofertilizer, while Gumi 20 M potassium biofertilizer had a positive effect on the parameters of barley seedlings under water stress conditions.
提高农业植物对非生物胁迫抗性的方法之一是使用具有抗胁迫和生长调节特性的生物肥料。它们提高了对有机肥料和土壤中氮和磷的吸收。本研究旨在研究在充分湿润和渗透胁迫条件下,用生物肥料预处理春大麦种子后,种子的播种品质、幼苗的形态指标。采用室内试验方法,研究了生物肥料在充分和不充分加湿条件下对春大麦幼苗生长的影响。大麦种子按照以下方案处理一次:1。对照(蒸馏水)10L/t;2.Gumi 20 M钾肥(0.4升/吨);3.Borogum-M复合物(0.2L/t);4.PEG 6000(100g/L);5.Gumi 20M(0.4L/t)+PEG 6000(100g/L);6.Borogum-M复合物(0.2L/t)+PEG6000(100g/L)。用生物肥料处理春大麦种子,发芽率和发芽率分别提高了35%和2%。使用PEG-6000模拟干旱时,种子的播种质量下降了4%。在充分增湿的条件下,所研究的生物肥料对大麦幼苗的地上部和根系重量具有复杂的正效应。此外,根、芽的平均长度和总根长都有所增加。根系数量变化不大。水分缺乏降低了Borogum-M复合生物肥料的有效性,而Gumi 20M钾生物肥料在水分胁迫条件下对大麦幼苗的参数有积极影响。
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引用次数: 1
Introducing Schizandra chinensis into the Southern Urals 将五味子引入乌拉尔南部
Pub Date : 2022-12-27 DOI: 10.22363/2312-797x-2022-17-4-448-454
R. A. Bilalova
Long-term introduction of Schizandra chinensis (Turcz.) Baill. was studied studying. The aim of the research was to assess the introduction resistance of Schisandra chinensis from the collection of lianas of South-Ural Botanical Garden-Institute, Ural Federal Research Center of the Russian Academy of Sciences. The 7 phenological growth stages were studied in 2014-2021. The prospects for introduction were determined according to P.I. Lapin and S.V. Sidneva, introduction resistance was assessed by the method of N.V. Trulevich. The following parameters were assessed: completeness of shoot development cycle, stability of rhythmic processes, vital state, life form, rate of ontogenesis, and renewal. According to the results of the research, lemongrass plants successfully passed the introduction tests. The species stably goes through all phases of seasonal development under cultivation, it is adapted to local climatic conditions and has high winter hardiness. Moreover, Schizandra chinensis is highly decorative throughout the growing season (more than 5 months). In accordance with the integral assessment, the taxon is assigned to the first group of prospects for introduction; Schisandra chinensis is a resistant plant. The crop is promising for use in recreational areas of Ufa and other settlements of the Southern Urals.
五味子(Schizandra chinensis)的长期引进Baill。被研究,被研究。以俄罗斯科学院乌拉尔联邦研究中心南乌拉尔植物园-研究所藤本植物为材料,研究了五味子的引种抗性。对2014-2021年7个物候生长期进行了研究。采用P.I. Lapin和S.V. Sidneva法确定了引种前景,采用N.V. Trulevich法评价了引种抗性。评估了以下参数:芽发育周期的完整性、节奏过程的稳定性、生命状态、生命形式、个体发生速率和更新。根据研究结果,柠檬草植物成功地通过了引种试验。该品种在栽培下稳定地经历了季节发展的各个阶段,适应当地气候条件,具有较高的冬季抗寒性。此外,五味子在整个生长季节(超过5个月)都具有很强的装饰性。根据综合评价,将该分类群划为第一组引种前景;五味子是一种抗性植物。这种作物有望用于乌法的休闲区和乌拉尔南部的其他定居点。
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引用次数: 0
Breeding value of safflower in arid conditions of the Northern Caspian 北里海干旱条件下红花的育种价值
Pub Date : 2022-12-27 DOI: 10.22363/2312-797x-2022-17-4-466-472
N. Zaitseva, E. Yachmeneva, I. Klimova
Low and unstable yields of the main oilseed crops do not create interest for their large-scale cultivation in conditions of the Northern Caspian - A strakhan region. For this reason, gross harvest of oilseeds is reducing significantly creating a vegetable oil shortage. Currently, safflower cultivation is relevant due to high drought resistance and quality indicators of safflower oil. 24 collection samples of safflower were studied on light chestnut soils of the Lower Volga region in 2019-2021. The experiments on safflower cultivars from seed collection of Vavilov Institute of Plant Industry were carried out at the experimental site of Precaspian Agrarian Federal Scientific Center of the Russian Academy of Sciences. Influence of the main abiotic and biotic environmental factors on morpho-biological and economic characteristics of safflower was determined. Based on the results obtained, the following promising samples having breeding value as a starting material for creation of new cultivars were identified: Gila, Shahalli-260, Tsambuli, Shirkas, Talan, Centr 70, Moldir, Nurlan, Aleksandrit, Shifo, Sinaloa-90. Productivity and oil content of the selected cultivars exceeded the indicators of cv. Astrakhan 747 (standard) by 0.431.06 t/ha and 1.753.02 %, respectively.
主要油籽作物产量低且不稳定,在里海北部-斯特拉罕地区的条件下,对大规模种植油籽作物没有兴趣。由于这个原因,油籽的总收成正在显著减少,造成植物油短缺。目前,红花油具有较高的抗旱性和质量指标,与红花栽培相关。2019-2021年在伏尔加河下游地区浅栗色土壤上对24份红花样品进行了研究。对瓦维洛夫植物工业研究所种子收集的红花品种进行试验,在俄罗斯科学院普里海农业联邦科学中心实验场进行。研究了主要的非生物和生物环境因子对红花形态生物学和经济性状的影响。根据所获得的结果,鉴定出以下具有选育价值的样品:Gila、Shahalli-260、Tsambuli、Shirkas、Talan、Centr 70、Moldir、Nurlan、Aleksandrit、Shifo、Sinaloa-90。所选品种的产量和含油量均超过cv指标。阿斯特拉罕747(标准)分别下降0.431.06吨/公顷和1.753.02%。
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引用次数: 0
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RUDN Journal of Agronomy and Animal Industries
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