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Adaptability of barley varieties in terms of fat content in grain under conditions of Krasnoyarsk forest-steppe 克拉斯诺亚尔斯克森林草原条件下大麦品种籽粒脂肪含量的适应性
Pub Date : 2023-01-01 DOI: 10.22363/2312-797x-2023-18-2-153-162
V. I. Polonsky, A. Sumina, S. A. Gerasimov
The aim of the study was to analyze possible relationship between fat content in grain of barley varieties and level of their stability for this biochemical trait. The work involved 15 varieties of hulled barley, which were grown for three years in conditions of the Krasnoyarsk foreststeppe (Minino farm, Emelyanovsky district, the Krasnoyarsk Territory). Climatic conditions in the research years differed significantly in terms of temperature and precipitation during the growing season. After harvesting plants, fat content in barley grain was measured by the chemical method according to GOST 13496.15-97. For barley varieties, two parameters of ecological plasticity (coefficient of variation; stress resistance index) and four stability parameters (indicator of level and stability of variety; homeostatic parameter; stability factor; index of selection value of variety) were calculated according to the indicated biochemical trait. It was found that Simon and Pallidum 4727 had the lowest value of plasticity and the highest value of stability (and, accordingly, the minimum sum of ranks) in terms of fat content in grain for the conditions of the Krasnoyarsk forest-steppe. G 18619, G 19589 and Dygyn were least valuable in terms of adaptability parameters with the maximum sum of ranks. It was shown that barley varieties with a higher fat content in grain were characterized by increased values of all stability parameters for this trait, and the indicated positive relationship was statistically proven for the Cs parameter. The result obtained shows that through the successful selection of barley for the maximum stability of grain fat content trait, oil content will not decrease.
本研究的目的是分析大麦籽粒脂肪含量与其生化性状稳定性之间的可能关系。这项工作涉及15个品种的去壳大麦,在克拉斯诺亚尔斯克森林草原(克拉斯诺亚尔斯克地区Emelyanovsky区的Minino农场)的条件下种植了三年。研究年份的气候条件在生长季节的温度和降水方面存在显著差异。收获植株后,采用GOST 13496.15-97的化学方法测定大麦籽粒脂肪含量。对于大麦品种,两个生态可塑性参数(变异系数;抗逆性指标)和4个稳定性参数(品种水平和稳定性指标;稳态参数;稳定系数;根据指示生化性状计算品种选择值指数。结果表明,在克拉斯诺亚尔斯克森林草原条件下,西蒙和白头草4727的籽粒脂肪含量可塑性最低,稳定性最高(因此等级总和最小)。G 18619、G 19589和Dygyn的自适应参数值最小,等级总和最大。结果表明,籽粒脂肪含量高的大麦品种,籽粒脂肪含量越高,籽粒脂肪含量的稳定性参数值越高,籽粒脂肪含量的稳定性参数值越高,籽粒脂肪含量的稳定性参数值与籽粒脂肪含量的稳定性参数值呈显著正相关。结果表明,通过对大麦籽粒脂肪含量性状的成功选育,籽粒含油量不会降低。
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引用次数: 0
Nano-Selenium-Mediated Alterations in Lipid Profile, Liver and Renal Functions, and Protein Parameters in Male Lambs: An Experimental Study 纳米硒介导的雄性羔羊脂质谱、肝肾功能和蛋白质参数改变:一项实验研究
Pub Date : 2023-01-01 DOI: 10.22363/2312-797x-2023-18-2-230-241
Karwan Anwar Hassan Aljaf, Marina N. Bolshakova
The impact of nano-selenium supplementation on lipid metabolism, liver enzymes, renal function, and protein levels in male lambs was investigated in this study during a 60-day period. Lambs were divided into two groups - control and experimental, which were administered nanoselenium orally at a dosage of 0.5 mg/ kg of feed once a day, daily. Each group consisted of five male lambs. According to the findings, cholesterol levels dropped significantly at days 45 (P 0.01) and 60 (P 0.05), whereas triglyceride levels significantly rose at days 15 and 30 (P 0.01). Throughout the trial, the levels of uric acid and creatinine were constant. At days 15 and 60, aspartate aminotransferase (AST) levels were significantly decreased (P 0.05), while at days 45 and 60, alkaline phosphatase (ALP) levels declined (P 0.01 and P 0.05, respectively). At days 15 and 30, albumin levels dropped significantly (0.05), but day 60 saw an increase in globulin levels significantly (P 0.05). The drop in AST and ALP levels might be a sign that liver function has possibly improved. However, the drop in albumin levels and rise in globulin levels raise the possibility that taking nano-selenium supplements may affect how proteins are metabolized. The findings of this study concluded that the effects of nano-selenium supplementation on lipid metabolism, liver enzymes, renal function, and protein levels in male lambs were time-dependent. In addition to possible improvements in liver function based on decreased AST and ALP levels and changes in albumin and globulin levels, the study also noticed an initial transient increase in triglyceride levels that was followed by a decline in cholesterol levels. To determine the time-dependent changes in liver function, liver enzymes, as well as renal function in response to nano-selenium supplementation in male lambs was objective of this study.
在为期60天的试验中,研究了纳米硒对雄性羔羊脂质代谢、肝酶、肾功能和蛋白质水平的影响。将羔羊分为对照组和试验组,每只羔羊口服纳米硒,剂量为0.5 mg/ kg,每日1次。每组5只公羊羔。结果表明,第45天和第60天胆固醇水平显著降低(p0.01),第15天和第30天甘油三酯水平显著升高(p0.01)。在整个试验过程中,尿酸和肌酐的水平是恒定的。第15和60天,天冬氨酸转氨酶(AST)水平极显著降低(P 0.05),第45和60天,碱性磷酸酶(ALP)水平分别降低(P 0.01和P 0.05)。第15、30天,白蛋白水平显著下降(0.05),第60天,球蛋白水平显著升高(P 0.05)。AST和ALP水平的下降可能是肝功能改善的信号。然而,白蛋白水平的下降和球蛋白水平的上升增加了服用纳米硒补充剂可能会影响蛋白质代谢的可能性。本研究结果表明,补充纳米硒对公羔羊脂质代谢、肝酶、肾功能和蛋白质水平的影响具有时间依赖性。除了基于AST和ALP水平降低以及白蛋白和球蛋白水平变化的肝功能可能改善之外,该研究还注意到甘油三酯水平最初的短暂升高,随后是胆固醇水平的下降。本研究旨在确定纳米硒对雄性羔羊肝功能、肝酶和肾功能的时间依赖性变化。
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引用次数: 0
Features of the course of hepatocardial syndrome in cats with hypertrophic cardiomyopathy 肥厚型心肌病猫肝心综合征的病程特点
Pub Date : 2023-01-01 DOI: 10.22363/2312-797x-2023-18-2-264-272
E. Sotnikova, Olesya A. Petrukhina, V. Byakhova, Vladimir D. Sibirtsev
The issues of changes in clinical, laboratory and instrumental parameters in cats with hepatocardial syndrome formed against the background of hypertrophic cardiomyopathy were studied. It is known that in high-bred cats with congestive heart failure, secondary hepatopathy can develop and progress. It was shown that hepatocardial syndrome occurs in 33.7 % of cats, out of the total number of patients with hypertrophic cardiomyopathy (n = 83). It has been established that hepatocardial complications in cats are a risk factor for a more severe course of hypertrophic cardiomyopathy. Hepatocardial syndrome in cats with hypertrophic cardiomyopathy is characterized by severe hypothermia, circulatory and respiratory failure. In sick animals, an increase in frequency of breathing during sleep was recorded (33.39.3 versus 17.91.8 times/min; p 0.001). Domestic cats with hepatocardial syndrome had a decrease in mean arterial blood pressure (100.219.3 versus 107.219.1 mm Hg; p 0.05), sinus tachycardia (200.319.6 17.8 times/min; p 0.001), which leads to a significant decrease in PQ intervals (57.99.9 versus 64.99.9 ms; p 0.001) and QT intervals (168.917, 2 vs 157.518.6 ms; p 0.001). Sick cats had a significant increase in the time of refilling of capillaries with blood, slowdown in intraventricular conduction, increase in voltage of ventricular and atrial complex on electrocardiograms, expansion of pulmonary vein, significant dilatation of left atrium, extreme concentric hypertrophy of left ventricle, increase in transverse contractility of myocardium of left ventricle and decrease in longitudinal contractility myocardium of left and right ventricles, cardiomyocyte cytolysis syndrome, cholestasis, and hypoalbuminemia
研究了在肥厚性心肌病背景下形成的肝心综合征猫的临床、实验室和仪器参数的变化问题。众所周知,在患有充血性心力衰竭的高级猫中,继发性肝病可以发展和进展。研究表明,在肥厚性心肌病患者总数(n = 83)中,33.7%的猫发生肝心综合征。已经确定,猫的肝心并发症是肥厚性心肌病更严重病程的危险因素。患有肥厚性心肌病的猫的肝心综合征的特征是严重的体温过低,循环和呼吸衰竭。在患病动物中,睡眠时呼吸频率增加(33.39.3次/分钟vs 17.91.8次/分钟;p 0.001)。患有肝心综合征的家猫平均动脉血压下降(100.219.3 vs 107.219.1 mm Hg;P 0.05),窦性心动过速(200.319.6 17.8次/min;p 0.001),这导致PQ间隔显著降低(57.99.9 ms vs 64.99.9 ms;p 0.001)和QT间期(168.917,2 vs 157.518.6 ms;p 0.001)。病猫毛细血管充血时间明显增加,室内传导减慢,心电图室房复合电压升高,肺静脉扩张,左心房明显扩张,左心室极度同心肥厚,左心室心肌横向收缩力增加,左右心室纵向收缩力下降。心肌细胞溶解综合征,胆汁淤积和低白蛋白血症
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引用次数: 0
Some aspects of testing cotton Gossypium hirsutum L. in conditions of the Astrakhan region 阿斯特拉罕地区棉棉试验的几个方面
Pub Date : 2023-01-01 DOI: 10.22363/2312-797x-2023-18-2-163-173
E. Myagkova
Cotton is a valuable crop for the Russian economy. Creation of the initial material for breeding work, as well as the introduction into production of high-yielding cotton varieties adapted to growing conditions are the main tasks set for Russian breeders. The study of agronomic traits of cotton varieties was carried out at Precaspian Agrarian Federal Scientific Center of the Russian Academy of Sciences, located in the north of the Astrakhan region, in 2017-2019. The research was performed in order to identify productivity potential, as well as to determine the adaptive capabilities to extreme climatic conditions of the Northern Caspian region. Cotton varieties were compared with the standard (AS-1). The results were processed according to methods of mathematical statistics. All varieties studied in the research had good indicators of agronomic traits. UZ-4 and Goliot varieties were characterized by the highest yield values - 3.0 and 2.6 t/ha, respectively.
棉花是俄罗斯经济的重要作物。为育种工作创造初始材料,以及将适应生长条件的高产棉花品种引入生产是俄罗斯育种者的主要任务。2017-2019年,位于阿斯特拉罕地区北部的俄罗斯科学院普里海农业联邦科学中心对棉花品种的农艺性状进行了研究。进行这项研究是为了确定生产力潜力,以及确定北里海地区对极端气候条件的适应能力。将棉花品种与标准(AS-1)进行比较。根据数理统计方法对结果进行了处理。研究品种农艺性状指标均较好。UZ-4和Goliot品种产量最高,分别为3.0 t/ha和2.6 t/ha。
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引用次数: 0
Molecular biological marker BCL-2: testis analysis in prenatal injection of estrogen to white laboratory mice 分子生物学标记BCL-2:白小鼠产前注射雌激素睾丸分析
Pub Date : 2023-01-01 DOI: 10.22363/2312-797x-2023-18-2-273-281
R. T. Sulaimanova, A. Kvochko
Immunohistochemical study is one of the modern methods of disease diagnostics used in practical veterinary practice, as well as in scientific developments in differential diagnostics of animal diseases of tumour and non-tumour nature. Prenatal influence of estrogens results in reproductive system disorders in an adult organism which is accompanied by parallel growth of steroid dependent cancers of the offspring: testicles and ovaries. The aim of the study was to perform immunohistochemical analysis of Bcl-2 marker during prenatal exposure to different doses of the synthetic estrogen analogue Sinestrol in the testes of the offspring of white non-pedigreed laboratory mice. After fertilization, the pregnant females were divided into 3 groups, one intact and two experimental groups. The intact group was unaffected (n = 10). The first experimental group, C-25 (n = 13), was injected with the estrogen drug Sinestrol in the form of a 2 % oil solution at a dose of 25 g/kg. The second experimental group (n = 13) was given the estrogen preparation Sinestrol in the form of 2 % oil solution in a dose of 40 mkg/kg. When the offspring reached sexual maturity, they were removed from the experiment. Immunohistochemical analysis was carried out on sections from paraffin blocks of testes of progeny intended for standard morphological study, the marker of apoptosis inhibitor Bcl-2 was determined on indices of cellular elements of male glands of progeny: spermatogonia, spermatocytes, sperm-tides, spermatozoa and Leydig cells. Expression of Bcl-2 marker upon exposure to the synthetic drug Sinestrol at doses of 25 and 40 g/kg showed that the number of positively stained cells in spermatogonia increased by 8.6 and 9.4 % respectively compared to the intact group. When the intact group was compared with experimental groups C-25 and C-40, the expression of Bcl-2 marker in spermatocyte cells and spermatozoa showed no difference, a slight increase in positively stained cells in spermatids was observed. Bcl-2 marker expression rate in experimental groups C 25 and C-40 decreased in Leydig cells by 56.0 (P 0.05) and 60.0 % (P 0.05), respectively. Administration of the synthetic estrogen analogue Sinestrol during fetal gland initiation resulted in impaired morphology in the testes in adulthood. The expression index of Bcl-2 marker in experimental groups C-25 and C-40 decreased in Leydig cells, resulting in apoptotic cell death, which is responsible for production of male sex hormone testosterone. The results can be used to select optimal doses of the synthetic estrogen analogue Sinestrol in the prenatal period.
免疫组织化学研究是一种现代疾病诊断方法,用于实际兽医实践,以及在动物疾病的肿瘤和非肿瘤性质的鉴别诊断的科学发展。雌激素的产前影响导致成年生物的生殖系统紊乱,并伴随着后代睾丸和卵巢中类固醇依赖性癌症的平行生长。本研究的目的是在产前暴露于不同剂量的合成雌激素类似物Sinestrol时,对白种非纯种实验室小鼠后代睾丸中的Bcl-2标志物进行免疫组织化学分析。受精后,将怀孕雌鼠分为3组,1个完整组和2个实验组。完整组未受影响(n = 10)。第一实验组C-25 (n = 13)以2%油溶液形式注射雌激素药物Sinestrol,剂量为25 g/kg。第二实验组(n = 13)给予雌激素制剂Sinestrol 2%油溶液,剂量为40 mkg/kg。当后代达到性成熟时,他们被从实验中移除。对子代睾丸石蜡切片进行标准形态学研究免疫组化分析,测定子代雄性腺体细胞成分指标:精原细胞、精母细胞、精潮细胞、精子和间质细胞中凋亡抑制剂Bcl-2的标记物。暴露于合成药物Sinestrol 25和40 g/kg剂量时,Bcl-2标记物的表达表明,精原细胞中阳性染色细胞的数量分别比完整组增加了8.6%和9.4%。完整组与实验组C-25、C-40比较,精母细胞和精子中Bcl-2标记物的表达无差异,精母细胞中阳性染色细胞略有增加。Bcl-2标志物在间质细胞中的表达率在实验组c25和c40中分别下降了56.0% (P < 0.05)和60.0% (P < 0.05)。在胎儿腺体起始阶段给予合成雌激素类似物辛雌醇导致成年后睾丸形态受损。实验组C-25和C-40间质细胞Bcl-2标记物表达指数下降,导致细胞凋亡死亡,该细胞负责雄性性激素睾酮的产生。该结果可用于产前选择合成雌激素类似物辛雌醇的最佳剂量。
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引用次数: 0
Macro and micromorphological features of prostate-vesical complex in dogs 犬前列腺膀胱复合体的宏观和微观形态学特征
Pub Date : 2023-01-01 DOI: 10.22363/2312-797x-2023-18-2-212-221
D.A. Golubtsova, N. Slesarenko
The results of macro and micromorphological studies of vesicoprostatic complex in dogs Canis familiaris aimed at establishing normative morphofunctional patterns and features of the urogenital apparatus were described. The morphometric parameters of the organ in non-castrated males, determined by somatotype and breed affiliation, were presented. Data reflecting the variability of the norm structure of the gland are basic in predicting the development of latent pathologies of prostate and in developing effective methods for their therapeutic correction.
本文描述了犬膀胱前列腺复合体的宏观和微观形态学研究结果,旨在建立规范的泌尿生殖器官形态功能模式和特征。在未阉割的雄性中,通过体型和品种归属确定了该器官的形态计量参数。反映腺体正常结构变异性的数据是预测前列腺潜在病变发展和制定有效治疗纠正方法的基础。
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引用次数: 0
Analysis of surgical correction of atlantoaxial instability in dogs 犬寰枢椎不稳的手术矫正分析
Pub Date : 2023-01-01 DOI: 10.22363/2312-797x-2023-18-2-241-249
I. Vilkovysky, I. A. Rusnak, S. A. Yagnikov, Nikolai V. Sakhno, S. B. Seleznev
Effectiveness of ventral surgical approach in correction of atlanto-axial instability in dogs of toy breeds was analyzed in the research. 135 clinical cases of surgical correction, the general concept of surgical access and implant placement, which minimize risks of iatrogenic complications, were studied. Ventral stabilization was carried out by forming an arthrodesis between atlas and epistropheus by inserting screws or spokes through articular structures and vertebral bodies from ventral surface and then fixing them with bone cement. In the studied method, there are also complications in form of failure of metal structures or damage to recurrent laryngeal nerve, but, according to statistics, the incidence of these complications in world practice does not exceed 20 %. Among the possible complications during the operation are death of animal because of sudden respiratory arrest associated with spinal cord injury, migration or breakage of implants, inadequate alignment of spine. In addition, implants may not be placed correctly causing chronic pain or damage to the spinal cord. As a result of the operation, 104 dogs fully recovered, partial preservation of neurological deficit was observed in 13 animals, 18 animals died in the early postoperative period. Complications that did not lead to a deterioration in neurological status and quality of life occurred in 17 animals. Analyzing the work done, the method of ventral stabilization in the treatment of atlanto-axial instability can be recommended as the most reliable and optimal method, since it is technically simple and has good long-term results. Statistical data showed good results, the method is effective and allows to fully return the animal to a quality life in more than 86 % of cases.
本研究分析了腹侧手术入路矫正玩具犬寰枢椎不稳的有效性。本文对135例临床手术矫治、手术通路和植入物的一般概念进行了研究,以减少医源性并发症的风险。腹侧稳定是通过从腹侧面通过关节结构和椎体插入螺钉或辐条形成寰椎与鼻尖间的关节融合术,然后用骨水泥固定。在本研究方法中,也存在金属结构失效或喉返神经损伤等并发症,但据统计,在世界实践中,这些并发症的发生率不超过20%。手术过程中可能出现的并发症包括动物因脊髓损伤引起的呼吸骤停而死亡、植入物移位或断裂、脊柱对齐不到位。此外,植入物可能放置不正确,导致慢性疼痛或脊髓损伤。术后104只狗完全恢复,13只动物神经功能缺损部分保留,18只动物术后早期死亡。17只动物出现并发症,但未导致神经状态和生活质量恶化。分析所做的工作,腹侧固定治疗寰枢椎不稳是最可靠和最佳的方法,因为它技术简单,长期效果好。统计数据显示效果良好,该方法有效,86%以上的病例可以使动物完全恢复到高质量的生活。
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引用次数: 0
Effectiveness of Farnesol for treatment of dog otitis complicated by Malassezia pachydermatis 法尼醇治疗犬中耳炎并发厚皮马拉色菌的疗效观察
Pub Date : 2023-01-01 DOI: 10.22363/2312-797x-2023-18-2-250-263
Ifarajimi R. Olabode, N. Sachivkina, E. Kiseleva, A. I. Shurov
The study involved 30 dogs with otitis complicated by Malassezia pachydermatis . The animals were divided into 2 groups: experimental (n = 15) and control (n = 15). The experimental group was prescribed veterinary ear drops Surolan + a solution of the drug Farnesol; the control group - Surolan. The study showed that when Farnesol is added to the treatment regimen for fungal otitis etiology in dogs, small changes in the clinical composition of the blood are recorded, characterized by an increase in the number of erythrocytes and hemoglobin, a decrease in the number of leukocytes, with a decrease in the indicators of eosinophils and rod-shaped neutrophils in the experiment compared with the control. In addition, the complete absence of Malassezia pachydermatis in the smears of the ear contents after two weeks of therapy with a combined drug was proved. That is, in the experimental group, there were no cases of the presence of yeast-like fungi (YLF) in the smear after combined therapy with Surolan + Farnesol. And in the control group of 15 animals, YLF were observed in two with microscopy of ear exudate smears, but not in high concentration. The use of a combination of Surolan and Farnesol in dogs of the experimental group led to a decrease in hyperemia, itching, edema, ear fetid fluid on the 5th7th days of treatment, and complete clinical recovery of the animals occurred by the 10th14th day of therapy. In the control group, only Surolan was used, and improvement of clinical condition occurred on days 1214, and recovery only after completion of the full course. As a result, both treatment regimens were successful, but the scheme with Farnesol gave faster results due to the wide spectrum of action of this drug: both antimicrobial and anti-inflammatory.
这项研究涉及30只患有中耳炎并患有马拉色菌厚皮炎的狗。将动物分为实验组(n = 15)和对照组(n = 15)。试验组患者给予兽用滴耳液苏洛兰+法尼醇溶液;对照组——苏罗兰。研究表明,在治疗犬真菌性中耳炎的治疗方案中加入法尼醇后,临床血液成分发生微小变化,表现为红细胞和血红蛋白数量增加,白细胞数量减少,嗜酸性粒细胞和杆状中性粒细胞指标较对照组下降。此外,经联合用药治疗两周后,证实耳内容物涂片中完全没有厚皮马拉色菌。即实验组经苏洛兰+法尼醇联合治疗后,涂片中未出现酵母菌样真菌(YLF)。对照组15只,2只耳渗出液涂片镜检可见YLF,但浓度不高。实验组狗在治疗第5 ~ 7天使用苏洛兰和法尼醇联合用药,充血、瘙痒、水肿、耳臭液减少,治疗第10 ~ 14天临床完全恢复。在对照组中,仅使用苏洛兰,临床状况在第1214天出现改善,在完成整个疗程后才恢复。结果,两种治疗方案都取得了成功,但法尼醇方案的效果更快,因为这种药物的作用范围很广:既抗微生物又抗炎。
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引用次数: 0
Effect of microbiological agents on spring barley cultivated in the north of the Astrakhan region 微生物制剂对阿斯特拉罕北部地区春大麦栽培的影响
Pub Date : 2022-12-28 DOI: 10.22363/2312-797x-2022-17-4-455-465
N. Naumova
The aim of the study was to determine effectiveness of the use of microbiological agents in spring barley cultivation. The experiments were conducted in the conditions of light chestnut soils in the north of the Astrakhan region, Chernoyarsky district, Solenoe Zaymishche village in 2018-2020. The use of this cultural method contributed to two-fold increase in the productivity of spring barley (3.5 t/ha), compared with the control (without any seed treatment - 1 .4 t/ha). The pre-sowing inoculation of seeds increased field germination and number of mature plants: by 75 and 26.8 % for Mizorin, 79 and 40.9 % for BisolbiFit, 78 and 37.6 % for Rizoagrin, respectively, in comparison with the control. Application of these microbiological agents in spring barley cultivation can be recommended to both small and large farms, which will help to rise the agriculture of the region to a high level of production of spring barley.
本研究的目的是确定在春大麦种植中使用微生物制剂的有效性。实验于2018-2020年在阿斯特拉罕地区北部Chernoyarsky区Solenoe Zaymishche村的浅栗色土壤条件下进行。与对照相比,使用这种培养方法使春大麦的生产力提高了两倍(3.5吨/公顷)(没有任何种子处理——1.4吨/公顷。播种前接种种子提高了田间发芽率和成熟植株数量:与对照相比,米佐林提高了75%和26.8%,BisolbiFit提高了79%和40.9%,Rizoagrin提高了78%和37.6%。这些微生物制剂在春大麦种植中的应用可以推荐给小型和大型农场,这将有助于将该地区的农业提高到高水平的春大麦生产。
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引用次数: 0
Mycotoxicological monitoring of feed and its role in prevention of animal mycotoxicoses 饲料真菌毒素监测及其在动物真菌毒素预防中的作用
Pub Date : 2022-12-27 DOI: 10.22363/2312-797x-2022-17-4-546-554
V. Dorozhkin, T. Gerunov, I. A. Simonova, L. Gerunova, Yana O. Kryuchek, Anna A. Tarasenko, E. Chigrinski
Mycotoxins can accumulate in raw materials of plant origin at different technological stages of its production. Most often, the producers of mycotoxins are fungi of the genera Aspergillus , Fusarium , Penicillium and some others. The clinical symptoms of mycotoxicoses vary significantly, and lethal outcomes are possible. For this reason, the mycotoxicological study of various types of feed under production conditions is an indispensable component of veterinary support of industrial animal husbandry. As part of this study, a retrospective analysis of the results of a mycotoxicological study of feed for different animal species was carried out in the Omsk region in 2017-2021. All feeds received by the Omsk Regional Veterinary Laboratory for 5 years for the determination of mycotoxins were examined for the presence of ochratoxin A, zearalenone, T-2 toxin, aflatoxin B 1, deoxynivalenol. It was established that almost 70 % of the studied samples contained mycotoxins, including their maximum allowable level was exceeded in 74 samples. Exceeding the permissible levels was noted for the content of T-2 toxin (34 samples), zearalenone (27 samples), ochratoxin A (6 samples), aflatoxin B 1 (4 samples) and deoxynivalenol (3 samples). The largest number of cases of contamination was recorded in the study of feed and feed mixtures. The greatest danger is the multiple contamination of feed with mycotoxins. This increases the risk of developing comorbid conditions and the spread of opportunistic infections.
真菌毒素可在植物原料生产的不同工艺阶段积累。大多数情况下,真菌毒素的生产者是曲霉属、镰刀菌属、青霉属和其他一些属的真菌。真菌中毒的临床症状差别很大,致死的结果是可能的。因此,在生产条件下对各类饲料进行真菌毒理学研究是工业化畜牧业兽医支持不可或缺的组成部分。作为本研究的一部分,2017-2021年在鄂木斯克地区对不同动物物种饲料的真菌毒理学研究结果进行了回顾性分析。对鄂木斯克地区兽医实验室5年来接收的所有饲料进行霉菌毒素检测,检查是否存在赭曲霉毒素A、玉米赤霉烯酮、T-2毒素、黄曲霉毒素b1、脱氧雪腐镰刀菌醇。经确定,近70%的研究样本含有真菌毒素,其中74个样本超过了其最大允许水平。T-2毒素(34份)、玉米赤霉烯酮(27份)、赭曲霉毒素A(6份)、黄曲霉毒素b1(4份)和脱氧雪腐菌醇(3份)含量超标。在对饲料和饲料混合物的研究中记录了最多的污染案例。最大的危险是饲料受到真菌毒素的多重污染。这增加了发生合并症和机会性感染传播的风险。
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RUDN Journal of Agronomy and Animal Industries
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