Zoological Letters最新文献

Absorption spectra of opsin-based pigments are tuned from the UV to the red regions by interactions of the chromophore with surrounding amino acid residues. Both vertebrates and invertebrates possess long-wavelength-sensitive (LWS) opsins, which underlie color vision involving "red" sensing. The LWS opsins have independently evolved in each lineage, which suggests the existence of diverse mechanisms in spectral tuning. In vertebrate LWS opsins, the mechanisms underlying spectral tuning have been well characterized by spectroscopic analyses with recombinant pigments of wild type (WT) and mutant opsins. However in invertebrate LWS opsins including insect ones, the mechanisms are largely unknown due to the difficulty in obtaining recombinant pigments. Here we have overcome the problem by analyzing heterologous action spectra based on light-dependent changes in the second messenger in opsin-expressing cultured cells. We found that WTs of two LWS opsins of the butterfly, Papilio xuthus, PxRh3 and PxRh1 have the wavelengths of the absorption maxima at around 570 nm and 540 nm, respectively. Analysis of a series of chimeric mutants showed that helix III is crucial to generating a difference of about 15 nm in the wavelength of absorption maxima of these LWS opsins. Further site-directed mutations in helix III revealed that amino acid residues at position 116 and 120 (bovine rhodopsin numbering system) are involved in the spectral tuning of PxRh1 and PxRh3, suggesting a different spectral tuning mechanism from that of primate LWS opsins.

Bryozoa is a large phylum of colonial aquatic suspension feeders. The boring ctenostome Hypophorella expansa is unique and inhabits parchment-like polychaete tubes. Morphological studies date back to the nineteenth century, but distinct adaptations to this specific habitat have not been properly analysed, which prompted us to reexamine the morphology of this recently encountered species. The colony of H. expansa is composed of elongated stolonal kenozooids with a distal capsule-like expansion. A median transversal muscle is present in the latter, and one autozooid is laterally attached to the capsule. Unique stolonal wrinkles are embedded in the thin parts of the stolons. Single autozooids are attached in an alternating right-left succession on subsequent stolons. Polypide morphology including digestive tract, muscular system and most parts of the nervous system are similar to other ctenostomes. The most obvious apomorphic features of Hypophorella are space balloons and the gnawing apparatus. The former are two fronto-lateral spherical structures on autozooids, which provide space inside the tube. The latter perforates layers of the polychaete tube wall and consists of two rows of cuticular teeth that, together with the entire vestibular wall, are introvertable during the protrusion-retraction process. The apertural muscles are in association with this gnawing apparatus heavily modified and show bilateral symmetry. Adaptations to the unique lifestyle of this species are thus evident in stolonal wrinkles, autozooidal space balloons and the gnawing apparatus. The growth pattern of the colony of H. expansa may aid in rapid colonization of the polychaete tube layers.

Background: CatSper is a sperm-specific calcium ion (Ca²⁺) channel, which regulates sperm flagellar beating by tuning cytoplasmic Ca²⁺ concentrations. Although this Ca²⁺ channel is essential for mammalian fertilization, recent bioinformatics analyses have revealed that genes encoding CatSper are heterogeneously distributed throughout the eukaryotes, including vertebrates. As this channel is activated by cytoplasmic alkalization in mammals and sea urchins, it has been proposed that the sperm-specific Na⁺/H⁺ exchanger (sNHE, a product of the SLC9C gene family) positively regulates its activity. In mouse, sNHE is functionally coupled to soluble adenylyl cyclase (sAC). CatSper, sNHE, and sAC have thus been considered functionally interconnected in the control of sperm motility, at least in mouse and sea urchin.

Results: We carried out a comparative genomic analysis to explore phylogenetic relationships among CatSper, sNHE and sAC in eukaryotes. We found that sNHE occurs only in Metazoa, although sAC occurs widely across eukaryotes. In animals, we found correlated and restricted distribution patterns of the three proteins, suggesting coevolution among them in the Metazoa. Namely, nearly all species in which CatSper is conserved also preserve sNHE and sAC. In contrast, in species without sAC, neither CatSper nor sNHE is conserved. On the other hand, the distribution of another testis-specific NHE (NHA, a product of the SLC9B gene family) does not show any apparent association with that of CatSper.

Conclusions: Our results suggest that CatSper, sNHE and sAC form prototypical machinery that functions in regulating sperm flagellar beating in Metazoa. In non-metazoan species, CatSper may be regulated by other H⁺ transporters, or its activity might be independent of cytoplasmic pH.