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Beyond hunger: Unveiling the rights to food in sub-Saharan Africa 超越饥饿:揭示撒哈拉以南非洲的食物权
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-14 DOI: 10.1002/fes3.530
Helen Onyeaka, Adedola S. Adeboye, Oluwaseun P. Bamidele, Isiguzoro Onyeoziri, Oluwafemi A. Adebo, Maria M. Adeyemi, Susan N. Thera-Sekgweng

This article explores the fundamental right to food, examining its legal framework, its relationship with other human rights, and the efforts being made globally to improve this right. It begins by looking at international and national laws that protect the right to food and examining their importance in ensuring food security and sovereignty. It then explores how food rights intersect with other rights, such as health, education, and labor, revealing the interrelated web of human rights. The article further highlights how the right to food is closely tied to the objectives of reducing poverty and promoting social justice, which are crucial components in the collective effort to achieve food security for all. Additionally, it evaluates the effectiveness of current policies and strategies in promoting the right to food, particularly in sub-Saharan Africa. The novelty of the article is that it brings into perspective, the encumbrance of food sovereignty in sub-Saharan Africa and concludes with potential solutions.

本文探讨基本食物权,研究其法律框架、与其他人权的关系以及全球为改善这一权利所做的努力。文章首先介绍了保护食物权的国际和国内法律,并探讨了这些法律在确保粮食安全和主权方面的重要性。然后,文章探讨了食物权如何与健康权、受教育权和劳动权等其他权利相互交叉,揭示了人权之间相互关联的网络。文章进一步强调了食物权是如何与减少贫困和促进社会公正的目标紧密联系在一起的,这些目标是实现人人享有粮食安全的集体努力的重要组成部分。此外,文章还评估了促进食物权的现行政策和战略的有效性,特别是在撒哈拉以南非洲地区。这篇文章的新颖之处在于,它透视了撒哈拉以南非洲粮食主权的障碍,并在最后提出了潜在的解决方案。
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引用次数: 0
The regulatory landscape of genetically engineered crops in Pakistan 巴基斯坦对转基因作物的监管情况
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-13 DOI: 10.1002/fes3.494
Muhammad Awais, Abdul Qayyum Rao, Sahar Sadaqat, Amina Yaqoob, Muhammad Saad Bhutta, Sana Shakoor, Aneela Yasmeen, Sara Ajmal, Hamed A. Ghramh, Khalid Ali Khan, Allah Bakhsh

Genome editing technology has become part and parcel of biotechnological approach to understand the genetic basis of different biological processes in an organism and for its application in different fields. The basic need of world food production demands a highly variable population of plants with maximum diversity of agronomic traits of interest. Genome editing is unique in the sense that it provides a direct approach to generate targeted variability. The worldwide availability of genome-edited crop varieties in the global market is approaching, whereas the decision to deal with genome-edited (GE) crops differently from genetically transformed crop varieties is under discussion all around the world. GE may add allochthonous or autochthonous sequences into the genome much like traditional transgenesis or cisgenesis. Considering this fact, the United States declared genome-edited crops as conventional. Many countries such as Japan, UK, China, and Brazil have already legislated their GE products and their products are near to get entry to the market. In Pakistan, academic and research-based scientific institutions have been working on genome-edited crops by using CRISPR (clustered regularly interspaced short palindromic repeats) Cas technology. As being a signatory of the Cartagena protocol, every case of GM (Genetically modified) or GE (Genetically Engineered) should be passed through the Technical Advisory Committee which will be decided on case-to-case bases depending upon its nature of random or targeted mutation. Pakistan Environmental Protection Act, Intellectual Property Organization of Pakistan Act, Seed amendment act, and PBR (The Plant Breeders' Rights Act) are the major constitutional acts employed and working for the regulations of GM alongside GE crops. Now, discussions of scientists, academicians and officials from regulatory body are in process for the design of GE crop commercialization policy in Pakistan. The CRISPR-Cas9 system is now the most in-demand piece of technology and has become an indispensable component of research and development in the field of life sciences.

基因组编辑技术已成为生物技术方法的重要组成部分,以了解生物体内不同生物过程的遗传基础,并将其应用于不同领域。世界粮食生产的基本需求要求植物群体具有高度的可变性,并具有最大限度的农艺性状多样性。基因组编辑的独特之处在于,它提供了一种直接生成目标变异性的方法。经过基因组编辑的作物品种即将在全球市场上市,而如何区别对待经过基因组编辑(GE)的作物和经过基因转化的作物品种,则是全世界都在讨论的问题。GE 可能会像传统的转基因或顺基因一样,在基因组中添加同源或自源序列。考虑到这一事实,美国宣布基因组编辑作物为常规作物。许多国家,如日本、英国、中国和巴西,已经对其基因工程产品进行了立法,其产品即将进入市场。在巴基斯坦,以学术和研究为基础的科研机构一直在利用 CRISPR(簇状规则间隔短回文重复序列)Cas 技术研究基因组编辑作物。作为《卡塔赫纳议定书》的签署国,每一例转基因(GM)或 GE(Genetically Engineered)都应通过技术咨询委员会,该委员会将根据随机突变或定向突变的性质逐案做出决定。巴基斯坦环境保护法》、《巴基斯坦知识产权组织法》、《种子修正法》和《植物育种者权利法》是用于管理转基因和基因工程作物的主要宪法法案。目前,科学家、院士和监管机构的官员正在讨论制定巴基斯坦的转基因作物商业化政策。CRISPR-Cas9 系统是目前最炙手可热的技术,已成为生命科学领域研发工作不可或缺的组成部分。
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引用次数: 0
Recruiting grape-isolated antagonistic yeasts for the sustainable bio-management of Botrytis cinerea on grapes 招募葡萄分离拮抗酵母,对葡萄上的灰葡萄孢菌进行可持续生物管理
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-09 DOI: 10.1002/fes3.528
Sebahat Oztekin, Funda Karbancioglu-Guler

Botrytis cinerea is the causative agent of grey mould disease in grapes, which was linked to significant postharvest losses. This study examined three grape-isolated yeasts (Metschnikowia aff. fructicola, Metschnikowia pulcherrima, and Hansenispora uvarum) through in vitro and in vivo tests on detached grape berries against grey mould, as well as the elucidation of their possible mechanisms of action. The antifungal mechanism of action of yeasts was determined by the lytic enzyme activity, inhibition of spore germination, biofilm activity, iron depletion, diffusible metabolites, wound-site colonisation, mycocin, and volatile organic compounds (VOCs) production. The highest in vitro efficacy (83.13%) was observed on M. aff. fructicola, followed by M. pulcherrima (82.10%) and H. uvarum (71.66%). Metschnikowia yeasts exhibited comparable enzyme activities, including protease, β-1,3 glucanase, gelatinase chitinase, and cellulase, while H. uvarum had a poor enzymatic activity with chitinase and gelatinase. M. aff. fructicola showed relatively higher iron depletion activity than M. pulcherrima, while M. pulcherrima outperformed via diffusible metabolites. All yeast cultures significantly reduced spore germination by at least 86%. Overall, M. aff. fructicola exhibited the highest biocontrol activity with its iron depletion, inhibition of conidial germination, biofilm formation, VOCs, and well colonisation on grape berries. M. aff. fructicola 1-UDM outperformed all other yeasts by significantly reducing disease incidence and lesion diameter values (93.4% and 94.3%, respectively). Remarkably, H. uvarum VOCs demonstrated potential as a biofumigant for suppressing grey mould. All yeasts are well adapted to their ecological niche to bio-protect grapes from grey mould disease.

灰葡萄孢菌是葡萄灰霉病的致病菌,与葡萄采后的重大损失有关。本研究通过对分离的葡萄果实进行抗灰霉病的体内外试验,研究了三种葡萄分离酵母菌(Metschnikowia aff. fructicola、Metschnikowia pulcherrima 和 Hansenispora uvarum),并阐明了它们可能的作用机制。酵母菌的抗真菌作用机制是通过裂解酶活性、孢子萌发抑制、生物膜活性、铁耗竭、可扩散代谢物、伤口定植、霉菌素和挥发性有机化合物(VOCs)的产生来确定的。对 M. aff. fructicola 的体外效力最高(83.13%),其次是 M. pulcherrima(82.10%)和 H. uvarum(71.66%)。Metschnikowia 酵母菌的酶活性相当,包括蛋白酶、β-1,3 葡聚糖酶、明胶酶、几丁质酶和纤维素酶,而 H. uvarum 的几丁质酶和明胶酶活性较差。M. aff. fructicola 比 M. pulcherrima 表现出更高的铁耗竭活性,而 M. pulcherrima 通过可扩散代谢物表现更佳。所有酵母培养物都能明显减少至少 86% 的孢子萌发。总体而言,果蝇酵母菌的生物控制活性最高,它能消耗铁、抑制分生孢子萌发、生物膜形成、挥发性有机化合物以及在葡萄果实上的良好定殖。M. aff. fructicola 1-UDM 的表现优于所有其他酵母菌,可显著降低病害发生率和病害直径值(分别为 93.4% 和 94.3%)。值得注意的是,H. uvarum VOCs 具有作为生物熏蒸剂抑制灰霉病的潜力。所有酵母菌都能很好地适应其生态位,为葡萄灰霉病提供生物保护。
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引用次数: 0
Impact of salicylic acid and sodium hydrosulfide applied singly or in combination on drought tolerance and grain yield in wheat plants 单独或联合使用水杨酸和硫氢化钠对小麦植物耐旱性和谷物产量的影响
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-09 DOI: 10.1002/fes3.532
Sabri Akin, Cengiz Kaya

Climate change-induced drought stress (DS) poses a significant threat to crop production, emphasizing the need for innovative strategies to mitigate its adverse effects. Prior studies have demonstrated the distinct capacities of salicylic acid (SA) and sodium hydrosulfide (NaHS) to augment plant resilience against drought-related stressors. However, little is known about how they work together or the specific processes by which they increase DS tolerance. The purpose of this research was to determine how SA and NaHS affected the performance of wheat plants during the growing seasons of 2021–2022 and 2022–2023, when there was a drought. The research employed a block-randomized experimental layout with split plots, where the primary factors included two irrigation levels: full irrigation (IW1, 100% of water requirement) and deficit irrigation (IW2, 50% of water requirement). Secondary factors included the application of mock control, 0.5 mM SA, and 0.3 mM NaHS, an H2S donor, either individually or in combination, administered before the onset of DS. The application of SA, NaHS, or their combination significantly enhanced wheat plant resistance to DS. Significant increases in a number of physiological markers, including proline content, relative water content (RWC), Fv/Fm, chlorophyll content, and antioxidant enzyme activity, demonstrated this improvement. Furthermore, in drought-stressed wheat plants, SA and NaHS treatments decreased the amounts of hydrogen peroxide (H2O2), malondialdehyde (MDA) content, and electrolyte leakage (EL). In conclusion, our study highlights the possibility of SA and NaHS, whether applied individually or in combination, to improve drought resistance in wheat plants, presenting a viable approach to lessen the effects of climate change on agricultural yield.

气候变化引起的干旱胁迫(DS)对作物生产构成了重大威胁,因此需要采取创新战略来减轻其不利影响。先前的研究已经证明,水杨酸(SA)和硫氢化钠(NaHS)具有不同的能力来增强植物对干旱相关胁迫的恢复力。然而,人们对这两种物质如何协同作用或它们提高 DS 耐受力的具体过程知之甚少。本研究旨在确定 SA 和 NaHS 在 2021-2022 年和 2022-2023 年干旱生长季节如何影响小麦植株的表现。研究采用了分块随机试验布局,主要因素包括两种灌溉水平:充分灌溉(IW1,需水量的 100%)和亏缺灌溉(IW2,需水量的 50%)。次要因素包括在 DS 开始前单独或联合施用模拟对照、0.5 mM SA 和 0.3 mM NaHS(一种 H2S 供体)。施用 SA、NaHS 或它们的组合能显著增强小麦植株对 DS 的抗性。脯氨酸含量、相对含水量 (RWC)、Fv/Fm、叶绿素含量和抗氧化酶活性等一系列生理指标的显著增加证明了这种改善。此外,在干旱胁迫的小麦植株中,SA 和 NaHS 处理降低了过氧化氢(H2O2)含量、丙二醛(MDA)含量和电解质渗漏(EL)。总之,我们的研究强调了单独或联合应用 SA 和 NaHS 提高小麦植株抗旱性的可能性,为减轻气候变化对农业产量的影响提供了一种可行的方法。
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引用次数: 0
Quantitative trait locus (QTL) mapping and transcriptome profiling identify QTLs and candidate genes associated with heat stress response during reproductive development in Camelina sativa 定量性状位点(QTL)绘图和转录组分析确定了与荠菜生殖发育过程中热胁迫响应相关的 QTL 和候选基因
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-09 DOI: 10.1002/fes3.531
Brian E. Smith, Sunny Kemmer, Samuel Decker, Chaofu Lu

Camelina sativa (L.) Crantz is a low-input oilseed crop that has great potential in providing sustainable feedstock for biofuels and bioproducts. Climate change is threatening production of camelina with rising global temperatures. Elucidating the genetic response to high temperatures is essential for successful breeding of heat-tolerant camelina varieties. Here, we report a combinatorial approach to identifying candidate genes associated with heat stress by quantitative trait locus (QTL) mapping and comparative transcriptome profiling. A population of recombinant inbred lines (RILs) was grown in a controlled growth chamber under the high-temperature regimes for 14 days beginning at the onset of the reproductive stage. Several traits related to seed production were evaluated at maturity. The QTL analysis identified several regions with co-located traits on chromosomes 8, 10, and 12. Two RILs with contrasting phenotypic responses to heat stress were chosen for gene expression profiling via RNA sequencing. Multiple pathways and genes were found to be strongly affected by heat stress, and many genes expressed differently between the two RILs. Several genes identified within the QTL regions were considered strong candidates that may control heat tolerance during reproduction in camelina. These studies provide resources for future studies that may assist in improving the heat tolerance of camelina.

荠菜(L. Camelina sativa (L.) Crantz)是一种低投入的油籽作物,在为生物燃料和生物产品提供可持续原料方面具有巨大潜力。随着全球气温的不断升高,气候变化正威胁着荠菜的生产。阐明基因对高温的反应对于成功培育耐热荠菜品种至关重要。在此,我们报告了一种通过定量性状位点(QTL)绘图和比较转录组图谱确定与热胁迫相关的候选基因的组合方法。重组近交系(RIL)群体从生殖期开始在高温条件下在受控生长室中生长了 14 天。在成熟期对与种子生产相关的几个性状进行了评估。QTL 分析确定了第 8、10 和 12 号染色体上具有共定位性状的几个区域。通过 RNA 测序,选择了两个对热胁迫的表型反应截然不同的 RIL 进行基因表达谱分析。研究发现,多种途径和基因受到热胁迫的强烈影响,许多基因在两个 RIL 之间表达不同。在 QTL 区域内发现的几个基因被认为是可能控制荠菜繁殖期间耐热性的有力候选基因。这些研究为今后的研究提供了资源,可能有助于提高荠菜的耐热性。
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引用次数: 0
Climate change and livestock production in Sub-Saharan Africa: Effects and transmission channels 气候变化与撒哈拉以南非洲的畜牧业生产:影响和传播渠道
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-09 DOI: 10.1002/fes3.521
Henri Aurélien Ateba Boyomo, Bruno Emmanuel Ongo Nkoa, Lucien Armel Awah Manga

This article assesses the effect of climate change on livestock production in Sub-Saharan Africa, for a sample of 45 countries over the period 2000–2021. Using a two-factor fixed effects panel data model, our results obtained by the two-way fixed effects estimator show that (i) climate change negatively influences livestock production through high temperatures, while abundant rainfall is beneficial. (ii) Through transmission channels, we find that maize price volatility exacerbates the negative effect of rising temperatures on livestock production, while it reduces the beneficial effect of abundant rainfall. Furthermore, we find that water availability mitigates the adverse effect of rising temperatures on livestock, while enhancing the beneficial effect of rainfall on livestock. Finally, we concede that conflicts reduce the beneficial effect of rainfall on livestock production. To increase livestock production in Sub-Saharan Africa, we recommend: the practice of pastoralism, based on the production of plants and fodder adapted to climate change, the improvement of animal nutrition, and the inclusion of breeders in the decision-making process in the cattle industry.

本文以撒哈拉以南非洲 45 个国家为样本,评估了 2000-2021 年间气候变化对畜牧业生产的影响。利用双因素固定效应面板数据模型,我们通过双向固定效应估计器得出的结果表明:(i) 气候变化通过高温对畜牧业生产产生负面影响,而充沛的降雨则对畜牧业生产有利。(ii) 通过传导渠道,我们发现玉米价格波动加剧了气温升高对畜牧业生产的负面影响,同时降低了充沛降雨的有利影响。此外,我们还发现,水的供应会减轻气温上升对牲畜的不利影响,同时增强降雨对牲畜的有利影响。最后,我们承认冲突降低了降雨对畜牧业生产的有利影响。为了提高撒哈拉以南非洲地区的牲畜产量,我们建议:在生产适应气候变化的植物和饲料的基础上实行畜牧业,改善动物营养,并让饲养者参与养牛业的决策过程。
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引用次数: 0
Effects of pesticide treatment against nematode disease on soil bacterial community structure and sweet potato yield and quality 防治线虫病的农药处理对土壤细菌群落结构以及甘薯产量和质量的影响
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-08 DOI: 10.1002/fes3.533
Kai Luo, Jie Zhu, Boyu Xia, Jie Zhang, Xiulin Su, Haixia Wen, Yuqi Li

Sweet potato stem nematode disease is a devastating disease, which seriously affects the yield and quality of sweet potato (Ipomoea batatas (L.) Lam.). At present, soil treatment with pesticides is mainly used to prevent sweet potato stem nematode disease. In this study, Illumina MiSeq high-throughput sequencing technology was used to analyze the diversity of soil bacterial and fungal communities treated with different pesticides. At the same time, high performance liquid chromatography was used to determine the pesticide residues in soil and sweet potato, and the impact on sweet potato yield was investigated. Kruskal–Wallis test was used to analyze the four α-diversity indexes of soil under different pesticide treatments, and the differences were not significant (p > 0.05), indicating that there was no significant difference in the diversity and abundance of bacterial and fungal communities in soil under different pesticide treatments. The results of principal coordinate analysis showed that there was no significant difference in bacterial and fungal community structure among different pesticide treatments (p > 0.05). Pesticide residue analysis and yield statistics of sweet potato showed that the residual amount of three kinds of pesticides in sweet potato did not exceed the maximum limit stipulated by the current National Standards for Food safety (GB2763-2021). When 5% chlorpyrifos phoxim particles were applied at 15.18 kg/acre, sweet potato was unaffected by pests and diseases basically, and the yield was the highest.

甘薯茎线虫病是一种毁灭性病害,严重影响甘薯(Ipomoea batatas (L.) Lam.)的产量和品质。目前,主要使用农药进行土壤处理来预防甘薯茎线虫病。本研究利用 Illumina MiSeq 高通量测序技术分析了使用不同农药处理的土壤细菌和真菌群落的多样性。同时,采用高效液相色谱法测定了土壤和甘薯中的农药残留量,并研究了其对甘薯产量的影响。采用 Kruskal-Wallis 检验分析了不同农药处理下土壤的四个 α 多样性指标,差异不显著(p > 0.05),表明不同农药处理下土壤中细菌和真菌群落的多样性和丰度无显著差异。主坐标分析结果表明,不同农药处理下细菌和真菌群落结构无显著差异(p > 0.05)。红薯农药残留分析和产量统计结果表明,红薯中 3 种农药的残留量均未超过现行《食品安全国家标准》(GB2763-2021)规定的最高限量。当 5%毒死蜱辛硫磷颗粒剂施用量为 15.18 公斤/亩时,甘薯基本不受病虫害影响,产量最高;当 5%毒死蜱辛硫磷颗粒剂施用量为 15.18 公斤/亩时,甘薯基本不受病虫害影响,产量最高。
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引用次数: 0
The integrated farming system is an environmentally friendly and cost-effective approach to the sustainability of agri-food systems in the modern era of the changing climate: A comprehensive review 在气候不断变化的现代,综合耕作制度是实现农业食品系统可持续性的一种环境友好型和具有成本效益的方法:全面回顾
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-08 DOI: 10.1002/fes3.534
Rakshit Bhagat, Sohan Singh Walia, Kartik Sharma, Rajbir Singh, Gurshaminder Singh, Akbar Hossain

The integrated farming system (IFS) is a holistic farming approach specially designed for small/marginal farmers to enhance the system productivity, profitability, and employment generation of their farm, ultimately ensuring their food and nutrition security for their livelihood. The mechanism of IFS offers various ecosystem services making farms environment-friendly, sustainable, and climate-resilient. IFS is characterized by the allocation of different agricultural components systematically in a single farm performing synergistically among themselves making the farm more productive, healthy, biodiversity enriched, and eco-friendly than simplified farms. Therefore, this review paper represents an extensive analysis of existing literature on IFS exploring its concept, components, and need, and emphasizing its potential for higher system productivity, profitability, and employment generation in a sustainable manner. Moreover, it develops a conceptualization of the relationship among different agricultural components in a single farm exploring its role in ecosystem services (reducing greenhouse gases, carbon sequestration, nutrient recycling, improving soil health, and biodiversity conservation).

综合耕作系统(IFS)是一种专门为小型/边缘农户设计的整体耕作方法,旨在提高其农场的系统生产力、盈利能力和创造就业机会,最终确保其生计所需的粮食和营养安全。综合农作法的机制提供了各种生态系统服务,使农场成为环境友好型、可持续发展型和气候适应型农场。综合农作战略的特点是在一个农场中系统地分配不同的农业组成部分,这些组成部分之间发挥协同作用,使农场比简化的农场更具生产力、更健康、生物多样性更丰富、更生态友好。因此,本综述文件对有关综合农作系统的现有文献进行了广泛分析,探讨了综合农作系统的概念、组成部分和需求,并强调了综合农作系统以可持续的方式提高系统生产力、盈利能力和创造就业机会的潜力。此外,本文还对单一农场中不同农业组成部分之间的关系进行了概念化,探讨了其在生态系统服务(减少温室气体、碳固存、养分循环、改善土壤健康和生物多样性保护)中的作用。
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引用次数: 0
Genome-wide association studies for rice seed germination under drought stress using 3VmrMLM 利用 3VmrMLM 进行干旱胁迫下水稻种子萌发的全基因组关联研究
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-06 DOI: 10.1002/fes3.529
Bin Yang, Shaona Chen, Zhao Zheng, Jiali Zeng, Jiping Liu, Hua Zhao, Yixiong Zheng

Improving seed germination under drought stress has the potential to increase crop yield in dry direct-seeded rice. In this study, a genome-wide association study (GWAS) was conducted to determine the genetic basis of seed germination under drought stress in a panel of 165 rice accessions using a multi-locus compressed variance-component mixed model, 3VmrMLM. A total of 33 quantitative trait nucleotides (QTNs) were identified in association with drought tolerance during seed germination. Around these QTNs, eight were found to be co-localized with known drought-related genes. Furthermore, a causal candidate gene, OsGA2ox5, which encodes a gibberellin (GA) 2-oxidase, was identified for QTN-Chr07_242906 by integrating linkage disequilibrium (LD) analysis and gene expression analysis. Knockout mutation of OsGA2ox5 resulted in delayed seed germination and retarded seedling growth under drought stress. RNA-seq analysis revealed that OsGA2ox5 regulates drought tolerance during seed germination mainly by modulating carbohydrate metabolism. Additionally, a non-synonymous coding variant in OsGA2ox5, SNP-Chr07_218893, was found to be strongly associated with drought tolerance level during seed germination. These findings provide valuable information for further exploration of novel drought-related genes and genetic improvement of rice drought tolerance in the future.

提高干旱胁迫下的种子萌发率有望增加旱直播水稻的产量。本研究采用多焦点压缩变异成分混合模型(3VmrMLM)进行了全基因组关联研究(GWAS),以确定 165 个水稻品种在干旱胁迫下种子萌发的遗传基础。共鉴定出 33 个与种子萌发期间耐旱性相关的数量性状核苷酸(QTN)。在这些 QTNs 中,有 8 个与已知的干旱相关基因共定位。此外,通过整合连锁不平衡(LD)分析和基因表达分析,为 QTN-Chr07_242906 确定了一个因果候选基因 OsGA2ox5,该基因编码赤霉素(GA)2-氧化酶。OsGA2ox5的基因敲除突变导致种子萌发延迟和干旱胁迫下幼苗生长迟缓。RNA-seq分析表明,OsGA2ox5主要通过调节碳水化合物代谢来调控种子萌发过程中的耐旱性。此外,研究还发现OsGA2ox5的一个非同义编码变异SNP-Chr07_218893与种子萌发过程中的耐旱性水平密切相关。这些发现为今后进一步探索新型干旱相关基因和遗传改良水稻耐旱性提供了宝贵的信息。
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引用次数: 0
The estimation of wheat yield combined with UAV canopy spectral and volumetric data 结合无人机冠层光谱和体积数据估算小麦产量
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-05 DOI: 10.1002/fes3.527
Tao Liu, Fei Wu, Nana Mou, Shaolong Zhu, Tianle Yang, Weijun Zhang, Hui Wang, Wei Wu, Yuanyuan Zhao, Chengming Sun, Zhaosheng Yao

Estimating wheat yield accurately is crucial for efficient agricultural management. While canopy spectral information is widely used for this purpose, the incorporation of canopy volumetric features (CVFs) remains underexplored. This study bridges this gap by utilizing unmanned aerial vehicle (UAV) multispectral imaging to capture images and elevation data of wheat at key developmental stages—gestation and flowering stages. We innovatively leveraged the elevation differences between these stages to calculate canopy height, develop a novel CVF, and refine the wheat yield prediction model across various wheat varieties, nitrogen fertilizer levels, and planting densities. The integration of canopy volume information significantly enhanced the accuracy of our yield prediction model, as evidenced by an R2 of 0.8380, an RMSE of 313.3 kg/ha, and an nRMSE of 11.33%. This approach not only yielded more precise estimates than models relying solely on spectral data but also introduced a novel dimension to wheat yield estimation methodologies. Our findings suggest that incorporating canopy volume characteristics can substantially optimize wheat yield prediction models, presenting a groundbreaking perspective for agricultural yield estimation.

准确估算小麦产量对高效农业管理至关重要。虽然冠层光谱信息已被广泛应用于这一目的,但冠层体积特征(CVF)仍未得到充分探索。本研究利用无人机(UAV)多光谱成像技术捕捉小麦关键发育阶段--孕穗期和开花期--的图像和高程数据,弥补了这一空白。我们创新性地利用这些阶段之间的海拔高度差异来计算冠层高度、开发新型 CVF,并完善不同小麦品种、氮肥水平和种植密度下的小麦产量预测模型。集成冠层体积信息大大提高了产量预测模型的准确性,R2 为 0.8380,RMSE 为 313.3 千克/公顷,nRMSE 为 11.33%。与仅依赖光谱数据的模型相比,这种方法不仅能获得更精确的估测结果,还为小麦产量估测方法引入了一个新的维度。我们的研究结果表明,结合冠层体积特征可以大大优化小麦产量预测模型,为农业产量估算提供了一个开创性的视角。
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Food and Energy Security
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