Taufiq Nawaz, Liping Gu, Shah Fahad, Shah Saud, Matthew Tom Harrison, Ruanbao Zhou
This review explores the potential of genetically engineering cyanobacteria with the aim of synthesizing high-value protein directly from atmospheric nitrogen. The article examines numerous techniques that may enhance protein synthesis in cyanobacteria, and discusses advantages, barriers, and opportunities for this strategy going forward. Genetic manipulation of cyanobacteria shows promise in sustainably raising protein production via reduced greenhouse gas emissions and lower dependence on synthetic fertilizers, but also potentially fewer environmental implications traditionally caused by conventional protein production methods. The article uncovers many difficulties in genetically modifying cyanobacteria for protein production. For example, genetically modified organisms (GMOs) have legal and regulatory ramifications that must be accounted for if ethical, moral and secure use of these technologies is to be ensured. Economic viability, too, must be evaluated, taking into consideration production costs, scalability, market demand and future market potential. We suggest that processing of cyanobacterial proteins in downstream stages need further development. Effective and economical methods are needed for protein extraction, purification, and formulation into commercially viable products. For successful application of cyanobacterial protein production at scale, such obstacles must be overcome. We conclude that genetic engineering of cyanobacteria for protein synthesis has a great deal of potential to offer a resource-effective and sustainable replacement for the synthesis of high-value proteins, so promoting a more sustainable and environmentally conscious future.
{"title":"Sustainable protein production through genetic engineering of cyanobacteria and use of atmospheric N2 gas","authors":"Taufiq Nawaz, Liping Gu, Shah Fahad, Shah Saud, Matthew Tom Harrison, Ruanbao Zhou","doi":"10.1002/fes3.536","DOIUrl":"https://doi.org/10.1002/fes3.536","url":null,"abstract":"<p>This review explores the potential of genetically engineering cyanobacteria with the aim of synthesizing high-value protein directly from atmospheric nitrogen. The article examines numerous techniques that may enhance protein synthesis in cyanobacteria, and discusses advantages, barriers, and opportunities for this strategy going forward. Genetic manipulation of cyanobacteria shows promise in sustainably raising protein production via reduced greenhouse gas emissions and lower dependence on synthetic fertilizers, but also potentially fewer environmental implications traditionally caused by conventional protein production methods. The article uncovers many difficulties in genetically modifying cyanobacteria for protein production. For example, genetically modified organisms (GMOs) have legal and regulatory ramifications that must be accounted for if ethical, moral and secure use of these technologies is to be ensured. Economic viability, too, must be evaluated, taking into consideration production costs, scalability, market demand and future market potential. We suggest that processing of cyanobacterial proteins in downstream stages need further development. Effective and economical methods are needed for protein extraction, purification, and formulation into commercially viable products. For successful application of cyanobacterial protein production at scale, such obstacles must be overcome. We conclude that genetic engineering of cyanobacteria for protein synthesis has a great deal of potential to offer a resource-effective and sustainable replacement for the synthesis of high-value proteins, so promoting a more sustainable and environmentally conscious future.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":"13 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.536","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140145687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andrea Magarini, Federico Colombo, Elena Cassani, Martina Ghidoli, Michela Landoni, Stefano Sangiorgio, Roberto Pilu
Fusarium disease and the consequent mycotoxin accumulation pose significant problem in maize cultivation, with fumonisins produced by Fusarium verticillioides posing a global health concern. To address this issue, a range of preventive measures (e.g. crop management techniques) can be implemented to minimize fungal infections. A promising strategy to counteract this issue involves the selection of genotypes with greater resistance to fungal pathogens. This approach has the potential to reduce the reliance on chemical inputs for controlling fungus growth or indirect infection vectors. Leveraging genetic approaches can help improve the economic sustainability of agriculture in the face of climate change challenges. In the present work, we assessed the importance of two husk leaf traits (coverage and number), their association with F. verticillioides infection, fumonisin content, and their potential influence on crop yield. The study was conducted in three locations in the North of Italy and 38 hybrids with varying resistance to F. Verticillioides were compared. The results obtained showed that husk coverage has a pivotal role not only in protecting maize ears from Fusarium infection but have also a significant impact on crop yield: a significant positive correlation was found between husk coverage and yield in all three locations (r = 0.33185; r = 0.51327 and r = 0.51207, respectively). Furthermore, in the field of Vicenza, a significant negative correlation was found between husk coverage and Fusarium severity (r = −0.41492). Husk coverage emerges as an important trait that merits inclusion in maize breeding programs, given its protective role against fungal infections and its favourable influence on both yield and grain quality.
{"title":"The role of husk traits in maize susceptibility to Fusarium verticillioides: A multi-location study in northern Italy","authors":"Andrea Magarini, Federico Colombo, Elena Cassani, Martina Ghidoli, Michela Landoni, Stefano Sangiorgio, Roberto Pilu","doi":"10.1002/fes3.537","DOIUrl":"https://doi.org/10.1002/fes3.537","url":null,"abstract":"<p>Fusarium disease and the consequent mycotoxin accumulation pose significant problem in maize cultivation, with fumonisins produced by <i>Fusarium verticillioides</i> posing a global health concern. To address this issue, a range of preventive measures (e.g. crop management techniques) can be implemented to minimize fungal infections. A promising strategy to counteract this issue involves the selection of genotypes with greater resistance to fungal pathogens. This approach has the potential to reduce the reliance on chemical inputs for controlling fungus growth or indirect infection vectors. Leveraging genetic approaches can help improve the economic sustainability of agriculture in the face of climate change challenges. In the present work, we assessed the importance of two husk leaf traits (coverage and number), their association with <i>F. verticillioides</i> infection, fumonisin content, and their potential influence on crop yield. The study was conducted in three locations in the North of Italy and 38 hybrids with varying resistance to <i>F. Verticillioides</i> were compared. The results obtained showed that husk coverage has a pivotal role not only in protecting maize ears from <i>Fusarium</i> infection but have also a significant impact on crop yield: a significant positive correlation was found between husk coverage and yield in all three locations (<i>r</i> = 0.33185; <i>r</i> = 0.51327 and <i>r</i> = 0.51207, respectively). Furthermore, in the field of Vicenza, a significant negative correlation was found between husk coverage and Fusarium severity (<i>r</i> = −0.41492). Husk coverage emerges as an important trait that merits inclusion in maize breeding programs, given its protective role against fungal infections and its favourable influence on both yield and grain quality.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":"13 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.537","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140123696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hongying Cui, Zhaoke Dong, Yingying Song, Wenxiu Guo, Yi Yu, Lili Li, Suhong Lv, Xingyuan Men
Agricultural intensification affects natural and crop ecosystems, and increases the risk of agricultural pests in agroecosystems. Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) is an important pest that damages a wide range of crops. However, the effect of agricultural intensification on the genetic diversity of this pest is still unclear. In this study, we investigated the effects of the composition and configuration of the landscape on the genetic diversity of the agricultural pest H. armigera based on cytochrome oxidase subunit I (COI) analyses. In total, 10 haplotypes were found in 2016 and 15 haplotypes in 2021 based on COI genes. The haplotype diversity and nucleotide diversity were the highest in the Anqiu (AQ) region during 2016 and in the Bincheng (BC) region during 2021. Haplotype 2 and haplotype 3 (Hap2 and Hap3) were the dominant haplotypes in the H. armigera population. Agricultural intensification had no effect on the genetic diversity of H. armigera between 2016 and 2021. Our study highlights the effect of agricultural intensification on the genetic diversity of H. armigera. Understanding the genetic consequences of agricultural intensification is essential for the green control of agricultural pests and the sustainable development of agriculture.
农业集约化会影响自然和作物生态系统,并增加农业生态系统中农业害虫的风险。Helicoverpa armigera Hübner(鳞翅目:夜蛾科)是一种危害多种作物的重要害虫。然而,农业集约化对该害虫遗传多样性的影响仍不清楚。在本研究中,我们基于细胞色素氧化酶亚单位 I(COI)分析,研究了景观的组成和配置对农业害虫 H. armigera 遗传多样性的影响。基于 COI 基因,2016 年共发现 10 个单倍型,2021 年发现 15 个单倍型。2016年安丘(AQ)地区的单倍型多样性和核苷酸多样性最高,2021年滨城(BC)地区的单倍型多样性和核苷酸多样性最高。单倍型2和单倍型3(Hap2和Hap3)是H. armigera种群的优势单倍型。在 2016 年至 2021 年期间,农业集约化对 H. armigera 的遗传多样性没有影响。我们的研究强调了农业集约化对 H. armigera 遗传多样性的影响。了解农业集约化的遗传后果对于农业害虫的绿色防控和农业的可持续发展至关重要。
{"title":"Effect of agricultural intensification on the genetic diversity of Helicoverpa armigera","authors":"Hongying Cui, Zhaoke Dong, Yingying Song, Wenxiu Guo, Yi Yu, Lili Li, Suhong Lv, Xingyuan Men","doi":"10.1002/fes3.525","DOIUrl":"https://doi.org/10.1002/fes3.525","url":null,"abstract":"<p>Agricultural intensification affects natural and crop ecosystems, and increases the risk of agricultural pests in agroecosystems. <i>Helicoverpa armigera</i> Hübner (Lepidoptera: Noctuidae) is an important pest that damages a wide range of crops. However, the effect of agricultural intensification on the genetic diversity of this pest is still unclear. In this study, we investigated the effects of the composition and configuration of the landscape on the genetic diversity of the agricultural pest <i>H. armigera</i> based on cytochrome oxidase subunit I (COI) analyses. In total, 10 haplotypes were found in 2016 and 15 haplotypes in 2021 based on COI genes. The haplotype diversity and nucleotide diversity were the highest in the Anqiu (AQ) region during 2016 and in the Bincheng (BC) region during 2021. Haplotype 2 and haplotype 3 (Hap2 and Hap3) were the dominant haplotypes in the <i>H. armigera</i> population. Agricultural intensification had no effect on the genetic diversity of <i>H. armigera</i> between 2016 and 2021. Our study highlights the effect of agricultural intensification on the genetic diversity of <i>H. armigera</i>. Understanding the genetic consequences of agricultural intensification is essential for the green control of agricultural pests and the sustainable development of agriculture.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":"13 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.525","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140114232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Robert MacKenzie, Sami Ullah, Christine H. Foyer
Many governments have set ambitious targets for tree planting and increased woodland cover as a key part of actions to reach net-zero carbon emissions by 2050. However, many uncertainties remain concerning how and where to expand tree cover, what species to plant, and how best to manage new plantations. Much contemporary forestry has been based on even-aged monocultures, largely because of perceived advantages for timber production. However, in order to play a key role in climate change mitigation future forests will have to achieve timber production (and wider ecosystem service provision) alongside resilience to biotic and abiotic challenge. It is therefore crucial that appropriate informed decisions are made with regard to the structure, composition, and planning of future forests, in order to provide sustainable solutions that provide environmental, economic, and health benefits to society. Genetically diverse, mixed, and irregular forests, with their higher biodiversity and niche complementarity, are promising new forest configurations for regulating the water cycle, storing carbon, and delivering other goods and services. In the following discussion, we have used UK information to illustrate the benefits of mixed woodland versus monocultures and highlighted current issues related to government initiatives and policies for current and future forests. However, similar issues and problems are encountered globally.
{"title":"Building forests for the future","authors":"A. Robert MacKenzie, Sami Ullah, Christine H. Foyer","doi":"10.1002/fes3.518","DOIUrl":"https://doi.org/10.1002/fes3.518","url":null,"abstract":"<p>Many governments have set ambitious targets for tree planting and increased woodland cover as a key part of actions to reach net-zero carbon emissions by 2050. However, many uncertainties remain concerning how and where to expand tree cover, what species to plant, and how best to manage new plantations. Much contemporary forestry has been based on even-aged monocultures, largely because of perceived advantages for timber production. However, in order to play a key role in climate change mitigation future forests will have to achieve timber production (and wider ecosystem service provision) alongside resilience to biotic and abiotic challenge. It is therefore crucial that appropriate informed decisions are made with regard to the structure, composition, and planning of future forests, in order to provide sustainable solutions that provide environmental, economic, and health benefits to society. Genetically diverse, mixed, and irregular forests, with their higher biodiversity and niche complementarity, are promising new forest configurations for regulating the water cycle, storing carbon, and delivering other goods and services. In the following discussion, we have used UK information to illustrate the benefits of mixed woodland versus monocultures and highlighted current issues related to government initiatives and policies for current and future forests. However, similar issues and problems are encountered globally.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":"13 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.518","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140043180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Salah Mohammed Abdulahi, Habtamu Legese Feyisa, Dereje Degu Ayen
Different social protection programs are designed in low-income countries to eradicate poverty and improve the food security of poor people. Currently, the Urban Productive Safety Net Program (UPSNP) is designed to support those who are living in poverty and face food insecurity with predictable and reliable support through food, cash, or vouchers. However, limited empirical evidence has been presented about the significant impact of the program on the well-being of households who participated in the program and the factors that affect the households' decision to participate in the program. Hence, this study aims to evaluate the impact of participation in UPSNP on well-being using household survey data gathered from three main cities of Ethiopia: Dire Dawa, Harar, and Jigjiga in 2022. This study employed both propensity score matching (PSM) and endogenous switching regression (ESR) models to assess the impact of the UPSNP. The result shows that the probability of a household's participation decision is determined by the age of the household head, number of children, savings, house ownership, employment status of the household head, and shock. Furthermore, we found a consistently positive impact across models, indicating that participation in UPSNP reduces poverty and increases food security of households.
{"title":"Impact of urban productive safety net programme on poverty reduction and food security: Evidence from major cities of Eastern Ethiopia (Dire Dawa, Harar and Jigjiga)","authors":"Salah Mohammed Abdulahi, Habtamu Legese Feyisa, Dereje Degu Ayen","doi":"10.1002/fes3.535","DOIUrl":"https://doi.org/10.1002/fes3.535","url":null,"abstract":"<p>Different social protection programs are designed in low-income countries to eradicate poverty and improve the food security of poor people. Currently, the Urban Productive Safety Net Program (UPSNP) is designed to support those who are living in poverty and face food insecurity with predictable and reliable support through food, cash, or vouchers. However, limited empirical evidence has been presented about the significant impact of the program on the well-being of households who participated in the program and the factors that affect the households' decision to participate in the program. Hence, this study aims to evaluate the impact of participation in UPSNP on well-being using household survey data gathered from three main cities of Ethiopia: Dire Dawa, Harar, and Jigjiga in 2022. This study employed both propensity score matching (PSM) and endogenous switching regression (ESR) models to assess the impact of the UPSNP. The result shows that the probability of a household's participation decision is determined by the age of the household head, number of children, savings, house ownership, employment status of the household head, and shock. Furthermore, we found a consistently positive impact across models, indicating that participation in UPSNP reduces poverty and increases food security of households.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":"13 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.535","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140024763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Helen Onyeaka, Adedola S. Adeboye, Oluwaseun P. Bamidele, Isiguzoro Onyeoziri, Oluwafemi A. Adebo, Maria M. Adeyemi, Susan N. Thera-Sekgweng
This article explores the fundamental right to food, examining its legal framework, its relationship with other human rights, and the efforts being made globally to improve this right. It begins by looking at international and national laws that protect the right to food and examining their importance in ensuring food security and sovereignty. It then explores how food rights intersect with other rights, such as health, education, and labor, revealing the interrelated web of human rights. The article further highlights how the right to food is closely tied to the objectives of reducing poverty and promoting social justice, which are crucial components in the collective effort to achieve food security for all. Additionally, it evaluates the effectiveness of current policies and strategies in promoting the right to food, particularly in sub-Saharan Africa. The novelty of the article is that it brings into perspective, the encumbrance of food sovereignty in sub-Saharan Africa and concludes with potential solutions.
{"title":"Beyond hunger: Unveiling the rights to food in sub-Saharan Africa","authors":"Helen Onyeaka, Adedola S. Adeboye, Oluwaseun P. Bamidele, Isiguzoro Onyeoziri, Oluwafemi A. Adebo, Maria M. Adeyemi, Susan N. Thera-Sekgweng","doi":"10.1002/fes3.530","DOIUrl":"https://doi.org/10.1002/fes3.530","url":null,"abstract":"<p>This article explores the fundamental right to food, examining its legal framework, its relationship with other human rights, and the efforts being made globally to improve this right. It begins by looking at international and national laws that protect the right to food and examining their importance in ensuring food security and sovereignty. It then explores how food rights intersect with other rights, such as health, education, and labor, revealing the interrelated web of human rights. The article further highlights how the right to food is closely tied to the objectives of reducing poverty and promoting social justice, which are crucial components in the collective effort to achieve food security for all. Additionally, it evaluates the effectiveness of current policies and strategies in promoting the right to food, particularly in sub-Saharan Africa. The novelty of the article is that it brings into perspective, the encumbrance of food sovereignty in sub-Saharan Africa and concludes with potential solutions.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":"13 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.530","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139739127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Awais, Abdul Qayyum Rao, Sahar Sadaqat, Amina Yaqoob, Muhammad Saad Bhutta, Sana Shakoor, Aneela Yasmeen, Sara Ajmal, Hamed A. Ghramh, Khalid Ali Khan, Allah Bakhsh
Genome editing technology has become part and parcel of biotechnological approach to understand the genetic basis of different biological processes in an organism and for its application in different fields. The basic need of world food production demands a highly variable population of plants with maximum diversity of agronomic traits of interest. Genome editing is unique in the sense that it provides a direct approach to generate targeted variability. The worldwide availability of genome-edited crop varieties in the global market is approaching, whereas the decision to deal with genome-edited (GE) crops differently from genetically transformed crop varieties is under discussion all around the world. GE may add allochthonous or autochthonous sequences into the genome much like traditional transgenesis or cisgenesis. Considering this fact, the United States declared genome-edited crops as conventional. Many countries such as Japan, UK, China, and Brazil have already legislated their GE products and their products are near to get entry to the market. In Pakistan, academic and research-based scientific institutions have been working on genome-edited crops by using CRISPR (clustered regularly interspaced short palindromic repeats) Cas technology. As being a signatory of the Cartagena protocol, every case of GM (Genetically modified) or GE (Genetically Engineered) should be passed through the Technical Advisory Committee which will be decided on case-to-case bases depending upon its nature of random or targeted mutation. Pakistan Environmental Protection Act, Intellectual Property Organization of Pakistan Act, Seed amendment act, and PBR (The Plant Breeders' Rights Act) are the major constitutional acts employed and working for the regulations of GM alongside GE crops. Now, discussions of scientists, academicians and officials from regulatory body are in process for the design of GE crop commercialization policy in Pakistan. The CRISPR-Cas9 system is now the most in-demand piece of technology and has become an indispensable component of research and development in the field of life sciences.
{"title":"The regulatory landscape of genetically engineered crops in Pakistan","authors":"Muhammad Awais, Abdul Qayyum Rao, Sahar Sadaqat, Amina Yaqoob, Muhammad Saad Bhutta, Sana Shakoor, Aneela Yasmeen, Sara Ajmal, Hamed A. Ghramh, Khalid Ali Khan, Allah Bakhsh","doi":"10.1002/fes3.494","DOIUrl":"https://doi.org/10.1002/fes3.494","url":null,"abstract":"<p>Genome editing technology has become part and parcel of biotechnological approach to understand the genetic basis of different biological processes in an organism and for its application in different fields. The basic need of world food production demands a highly variable population of plants with maximum diversity of agronomic traits of interest. Genome editing is unique in the sense that it provides a direct approach to generate targeted variability. The worldwide availability of genome-edited crop varieties in the global market is approaching, whereas the decision to deal with genome-edited (GE) crops differently from genetically transformed crop varieties is under discussion all around the world. GE may add allochthonous or autochthonous sequences into the genome much like traditional transgenesis or cisgenesis. Considering this fact, the United States declared genome-edited crops as conventional. Many countries such as Japan, UK, China, and Brazil have already legislated their GE products and their products are near to get entry to the market. In Pakistan, academic and research-based scientific institutions have been working on genome-edited crops by using CRISPR (clustered regularly interspaced short palindromic repeats) Cas technology. As being a signatory of the Cartagena protocol, every case of GM (Genetically modified) or GE (Genetically Engineered) should be passed through the Technical Advisory Committee which will be decided on case-to-case bases depending upon its nature of random or targeted mutation. Pakistan Environmental Protection Act, Intellectual Property Organization of Pakistan Act, Seed amendment act, and PBR (The Plant Breeders' Rights Act) are the major constitutional acts employed and working for the regulations of GM alongside GE crops. Now, discussions of scientists, academicians and officials from regulatory body are in process for the design of GE crop commercialization policy in Pakistan. The CRISPR-Cas9 system is now the most in-demand piece of technology and has become an indispensable component of research and development in the field of life sciences.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":"13 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139727690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Botrytis cinerea is the causative agent of grey mould disease in grapes, which was linked to significant postharvest losses. This study examined three grape-isolated yeasts (Metschnikowia aff. fructicola, Metschnikowia pulcherrima, and Hansenispora uvarum) through in vitro and in vivo tests on detached grape berries against grey mould, as well as the elucidation of their possible mechanisms of action. The antifungal mechanism of action of yeasts was determined by the lytic enzyme activity, inhibition of spore germination, biofilm activity, iron depletion, diffusible metabolites, wound-site colonisation, mycocin, and volatile organic compounds (VOCs) production. The highest in vitro efficacy (83.13%) was observed on M. aff. fructicola, followed by M. pulcherrima (82.10%) and H. uvarum (71.66%). Metschnikowia yeasts exhibited comparable enzyme activities, including protease, β-1,3 glucanase, gelatinase chitinase, and cellulase, while H. uvarum had a poor enzymatic activity with chitinase and gelatinase. M. aff. fructicola showed relatively higher iron depletion activity than M. pulcherrima, while M. pulcherrima outperformed via diffusible metabolites. All yeast cultures significantly reduced spore germination by at least 86%. Overall, M. aff. fructicola exhibited the highest biocontrol activity with its iron depletion, inhibition of conidial germination, biofilm formation, VOCs, and well colonisation on grape berries. M. aff. fructicola 1-UDM outperformed all other yeasts by significantly reducing disease incidence and lesion diameter values (93.4% and 94.3%, respectively). Remarkably, H. uvarum VOCs demonstrated potential as a biofumigant for suppressing grey mould. All yeasts are well adapted to their ecological niche to bio-protect grapes from grey mould disease.
灰葡萄孢菌是葡萄灰霉病的致病菌,与葡萄采后的重大损失有关。本研究通过对分离的葡萄果实进行抗灰霉病的体内外试验,研究了三种葡萄分离酵母菌(Metschnikowia aff. fructicola、Metschnikowia pulcherrima 和 Hansenispora uvarum),并阐明了它们可能的作用机制。酵母菌的抗真菌作用机制是通过裂解酶活性、孢子萌发抑制、生物膜活性、铁耗竭、可扩散代谢物、伤口定植、霉菌素和挥发性有机化合物(VOCs)的产生来确定的。对 M. aff. fructicola 的体外效力最高(83.13%),其次是 M. pulcherrima(82.10%)和 H. uvarum(71.66%)。Metschnikowia 酵母菌的酶活性相当,包括蛋白酶、β-1,3 葡聚糖酶、明胶酶、几丁质酶和纤维素酶,而 H. uvarum 的几丁质酶和明胶酶活性较差。M. aff. fructicola 比 M. pulcherrima 表现出更高的铁耗竭活性,而 M. pulcherrima 通过可扩散代谢物表现更佳。所有酵母培养物都能明显减少至少 86% 的孢子萌发。总体而言,果蝇酵母菌的生物控制活性最高,它能消耗铁、抑制分生孢子萌发、生物膜形成、挥发性有机化合物以及在葡萄果实上的良好定殖。M. aff. fructicola 1-UDM 的表现优于所有其他酵母菌,可显著降低病害发生率和病害直径值(分别为 93.4% 和 94.3%)。值得注意的是,H. uvarum VOCs 具有作为生物熏蒸剂抑制灰霉病的潜力。所有酵母菌都能很好地适应其生态位,为葡萄灰霉病提供生物保护。
{"title":"Recruiting grape-isolated antagonistic yeasts for the sustainable bio-management of Botrytis cinerea on grapes","authors":"Sebahat Oztekin, Funda Karbancioglu-Guler","doi":"10.1002/fes3.528","DOIUrl":"https://doi.org/10.1002/fes3.528","url":null,"abstract":"<p><i>Botrytis cinerea</i> is the causative agent of grey mould disease in grapes, which was linked to significant postharvest losses. This study examined three grape-isolated yeasts (<i>Metschnikowia</i> aff. <i>fructicola</i>, <i>Metschnikowia pulcherrima</i>, and <i>Hansenispora uvarum</i>) through <i>in vitro</i> and <i>in vivo</i> tests on detached grape berries against grey mould, as well as the elucidation of their possible mechanisms of action. The antifungal mechanism of action of yeasts was determined by the lytic enzyme activity, inhibition of spore germination, biofilm activity, iron depletion, diffusible metabolites, wound-site colonisation, mycocin, and volatile organic compounds (VOCs) production. The highest <i>in vitro</i> efficacy (83.13%) was observed on <i>M.</i> aff. <i>fructicola</i>, followed by <i>M. pulcherrima</i> (82.10%) and <i>H. uvarum</i> (71.66%). <i>Metschnikowia</i> yeasts exhibited comparable enzyme activities, including protease, β-1,3 glucanase, gelatinase chitinase, and cellulase, while <i>H. uvarum</i> had a poor enzymatic activity with chitinase and gelatinase. <i>M.</i> aff. <i>fructicola</i> showed relatively higher iron depletion activity than <i>M. pulcherrima</i>, while <i>M. pulcherrima</i> outperformed via diffusible metabolites. All yeast cultures significantly reduced spore germination by at least 86%. Overall, <i>M.</i> aff. <i>fructicola</i> exhibited the highest biocontrol activity with its iron depletion, inhibition of conidial germination, biofilm formation, VOCs, and well colonisation on grape berries. <i>M.</i> aff. <i>fructicola</i> 1-UDM outperformed all other yeasts by significantly reducing disease incidence and lesion diameter values (93.4% and 94.3%, respectively). Remarkably, <i>H. uvarum</i> VOCs demonstrated potential as a biofumigant for suppressing grey mould. All yeasts are well adapted to their ecological niche to bio-protect grapes from grey mould disease.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":"13 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.528","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139716823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Climate change-induced drought stress (DS) poses a significant threat to crop production, emphasizing the need for innovative strategies to mitigate its adverse effects. Prior studies have demonstrated the distinct capacities of salicylic acid (SA) and sodium hydrosulfide (NaHS) to augment plant resilience against drought-related stressors. However, little is known about how they work together or the specific processes by which they increase DS tolerance. The purpose of this research was to determine how SA and NaHS affected the performance of wheat plants during the growing seasons of 2021–2022 and 2022–2023, when there was a drought. The research employed a block-randomized experimental layout with split plots, where the primary factors included two irrigation levels: full irrigation (IW1, 100% of water requirement) and deficit irrigation (IW2, 50% of water requirement). Secondary factors included the application of mock control, 0.5 mM SA, and 0.3 mM NaHS, an H2S donor, either individually or in combination, administered before the onset of DS. The application of SA, NaHS, or their combination significantly enhanced wheat plant resistance to DS. Significant increases in a number of physiological markers, including proline content, relative water content (RWC), Fv/Fm, chlorophyll content, and antioxidant enzyme activity, demonstrated this improvement. Furthermore, in drought-stressed wheat plants, SA and NaHS treatments decreased the amounts of hydrogen peroxide (H2O2), malondialdehyde (MDA) content, and electrolyte leakage (EL). In conclusion, our study highlights the possibility of SA and NaHS, whether applied individually or in combination, to improve drought resistance in wheat plants, presenting a viable approach to lessen the effects of climate change on agricultural yield.
气候变化引起的干旱胁迫(DS)对作物生产构成了重大威胁,因此需要采取创新战略来减轻其不利影响。先前的研究已经证明,水杨酸(SA)和硫氢化钠(NaHS)具有不同的能力来增强植物对干旱相关胁迫的恢复力。然而,人们对这两种物质如何协同作用或它们提高 DS 耐受力的具体过程知之甚少。本研究旨在确定 SA 和 NaHS 在 2021-2022 年和 2022-2023 年干旱生长季节如何影响小麦植株的表现。研究采用了分块随机试验布局,主要因素包括两种灌溉水平:充分灌溉(IW1,需水量的 100%)和亏缺灌溉(IW2,需水量的 50%)。次要因素包括在 DS 开始前单独或联合施用模拟对照、0.5 mM SA 和 0.3 mM NaHS(一种 H2S 供体)。施用 SA、NaHS 或它们的组合能显著增强小麦植株对 DS 的抗性。脯氨酸含量、相对含水量 (RWC)、Fv/Fm、叶绿素含量和抗氧化酶活性等一系列生理指标的显著增加证明了这种改善。此外,在干旱胁迫的小麦植株中,SA 和 NaHS 处理降低了过氧化氢(H2O2)含量、丙二醛(MDA)含量和电解质渗漏(EL)。总之,我们的研究强调了单独或联合应用 SA 和 NaHS 提高小麦植株抗旱性的可能性,为减轻气候变化对农业产量的影响提供了一种可行的方法。
{"title":"Impact of salicylic acid and sodium hydrosulfide applied singly or in combination on drought tolerance and grain yield in wheat plants","authors":"Sabri Akin, Cengiz Kaya","doi":"10.1002/fes3.532","DOIUrl":"https://doi.org/10.1002/fes3.532","url":null,"abstract":"<p>Climate change-induced drought stress (DS) poses a significant threat to crop production, emphasizing the need for innovative strategies to mitigate its adverse effects. Prior studies have demonstrated the distinct capacities of salicylic acid (SA) and sodium hydrosulfide (NaHS) to augment plant resilience against drought-related stressors. However, little is known about how they work together or the specific processes by which they increase DS tolerance. The purpose of this research was to determine how SA and NaHS affected the performance of wheat plants during the growing seasons of 2021–2022 and 2022–2023, when there was a drought. The research employed a block-randomized experimental layout with split plots, where the primary factors included two irrigation levels: full irrigation (IW1, 100% of water requirement) and deficit irrigation (IW2, 50% of water requirement). Secondary factors included the application of mock control, 0.5 mM SA, and 0.3 mM NaHS, an H<sub>2</sub>S donor, either individually or in combination, administered before the onset of DS. The application of SA, NaHS, or their combination significantly enhanced wheat plant resistance to DS. Significant increases in a number of physiological markers, including proline content, relative water content (RWC), Fv/Fm, chlorophyll content, and antioxidant enzyme activity, demonstrated this improvement. Furthermore, in drought-stressed wheat plants, SA and NaHS treatments decreased the amounts of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), malondialdehyde (MDA) content, and electrolyte leakage (EL). In conclusion, our study highlights the possibility of SA and NaHS, whether applied individually or in combination, to improve drought resistance in wheat plants, presenting a viable approach to lessen the effects of climate change on agricultural yield.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":"13 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.532","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139716826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brian E. Smith, Sunny Kemmer, Samuel Decker, Chaofu Lu
Camelina sativa (L.) Crantz is a low-input oilseed crop that has great potential in providing sustainable feedstock for biofuels and bioproducts. Climate change is threatening production of camelina with rising global temperatures. Elucidating the genetic response to high temperatures is essential for successful breeding of heat-tolerant camelina varieties. Here, we report a combinatorial approach to identifying candidate genes associated with heat stress by quantitative trait locus (QTL) mapping and comparative transcriptome profiling. A population of recombinant inbred lines (RILs) was grown in a controlled growth chamber under the high-temperature regimes for 14 days beginning at the onset of the reproductive stage. Several traits related to seed production were evaluated at maturity. The QTL analysis identified several regions with co-located traits on chromosomes 8, 10, and 12. Two RILs with contrasting phenotypic responses to heat stress were chosen for gene expression profiling via RNA sequencing. Multiple pathways and genes were found to be strongly affected by heat stress, and many genes expressed differently between the two RILs. Several genes identified within the QTL regions were considered strong candidates that may control heat tolerance during reproduction in camelina. These studies provide resources for future studies that may assist in improving the heat tolerance of camelina.
{"title":"Quantitative trait locus (QTL) mapping and transcriptome profiling identify QTLs and candidate genes associated with heat stress response during reproductive development in Camelina sativa","authors":"Brian E. Smith, Sunny Kemmer, Samuel Decker, Chaofu Lu","doi":"10.1002/fes3.531","DOIUrl":"https://doi.org/10.1002/fes3.531","url":null,"abstract":"<p><i>Camelina sativa</i> (L.) Crantz is a low-input oilseed crop that has great potential in providing sustainable feedstock for biofuels and bioproducts. Climate change is threatening production of camelina with rising global temperatures. Elucidating the genetic response to high temperatures is essential for successful breeding of heat-tolerant camelina varieties. Here, we report a combinatorial approach to identifying candidate genes associated with heat stress by quantitative trait locus (QTL) mapping and comparative transcriptome profiling. A population of recombinant inbred lines (RILs) was grown in a controlled growth chamber under the high-temperature regimes for 14 days beginning at the onset of the reproductive stage. Several traits related to seed production were evaluated at maturity. The QTL analysis identified several regions with co-located traits on chromosomes 8, 10, and 12. Two RILs with contrasting phenotypic responses to heat stress were chosen for gene expression profiling via RNA sequencing. Multiple pathways and genes were found to be strongly affected by heat stress, and many genes expressed differently between the two RILs. Several genes identified within the QTL regions were considered strong candidates that may control heat tolerance during reproduction in camelina. These studies provide resources for future studies that may assist in improving the heat tolerance of camelina.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":"13 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.531","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139716822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}