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Sustainable protein production through genetic engineering of cyanobacteria and use of atmospheric N2 gas 通过蓝藻基因工程和利用大气中的 N2 气体实现可持续蛋白质生产
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-18 DOI: 10.1002/fes3.536
Taufiq Nawaz, Liping Gu, Shah Fahad, Shah Saud, Matthew Tom Harrison, Ruanbao Zhou

This review explores the potential of genetically engineering cyanobacteria with the aim of synthesizing high-value protein directly from atmospheric nitrogen. The article examines numerous techniques that may enhance protein synthesis in cyanobacteria, and discusses advantages, barriers, and opportunities for this strategy going forward. Genetic manipulation of cyanobacteria shows promise in sustainably raising protein production via reduced greenhouse gas emissions and lower dependence on synthetic fertilizers, but also potentially fewer environmental implications traditionally caused by conventional protein production methods. The article uncovers many difficulties in genetically modifying cyanobacteria for protein production. For example, genetically modified organisms (GMOs) have legal and regulatory ramifications that must be accounted for if ethical, moral and secure use of these technologies is to be ensured. Economic viability, too, must be evaluated, taking into consideration production costs, scalability, market demand and future market potential. We suggest that processing of cyanobacterial proteins in downstream stages need further development. Effective and economical methods are needed for protein extraction, purification, and formulation into commercially viable products. For successful application of cyanobacterial protein production at scale, such obstacles must be overcome. We conclude that genetic engineering of cyanobacteria for protein synthesis has a great deal of potential to offer a resource-effective and sustainable replacement for the synthesis of high-value proteins, so promoting a more sustainable and environmentally conscious future.

本综述探讨了蓝藻基因工程的潜力,目的是直接利用大气中的氮合成高价值蛋白质。文章探讨了可提高蓝藻蛋白质合成的多种技术,并讨论了这一战略的优势、障碍和未来机遇。蓝藻的遗传操作有望通过减少温室气体排放和降低对合成肥料的依赖来可持续地提高蛋白质产量,同时还可能减少传统蛋白质生产方法对环境造成的影响。文章揭示了转基因蓝藻生产蛋白质的许多困难。例如,转基因生物(GMOs)具有法律和监管方面的影响,如果要确保在伦理、道德和安全的前提下使用这些技术,就必须考虑到这些影响。此外,还必须对经济可行性进行评估,同时考虑生产成本、可扩展性、市场需求和未来市场潜力。我们建议,蓝藻蛋白质下游阶段的加工需要进一步开发。蛋白质的提取、纯化和配制成商业上可行的产品需要有效而经济的方法。要成功实现蓝藻蛋白质的规模化生产,必须克服这些障碍。我们的结论是,用于合成蛋白质的蓝藻基因工程具有巨大的潜力,可为高价值蛋白质的合成提供一种资源有效且可持续的替代方法,从而促进更具可持续性和环保意识的未来。
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引用次数: 0
The role of husk traits in maize susceptibility to Fusarium verticillioides: A multi-location study in northern Italy 外皮性状在玉米对疣镰刀菌的易感性中的作用:意大利北部多地研究
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-14 DOI: 10.1002/fes3.537
Andrea Magarini, Federico Colombo, Elena Cassani, Martina Ghidoli, Michela Landoni, Stefano Sangiorgio, Roberto Pilu

Fusarium disease and the consequent mycotoxin accumulation pose significant problem in maize cultivation, with fumonisins produced by Fusarium verticillioides posing a global health concern. To address this issue, a range of preventive measures (e.g. crop management techniques) can be implemented to minimize fungal infections. A promising strategy to counteract this issue involves the selection of genotypes with greater resistance to fungal pathogens. This approach has the potential to reduce the reliance on chemical inputs for controlling fungus growth or indirect infection vectors. Leveraging genetic approaches can help improve the economic sustainability of agriculture in the face of climate change challenges. In the present work, we assessed the importance of two husk leaf traits (coverage and number), their association with F. verticillioides infection, fumonisin content, and their potential influence on crop yield. The study was conducted in three locations in the North of Italy and 38 hybrids with varying resistance to F. Verticillioides were compared. The results obtained showed that husk coverage has a pivotal role not only in protecting maize ears from Fusarium infection but have also a significant impact on crop yield: a significant positive correlation was found between husk coverage and yield in all three locations (r = 0.33185; r = 0.51327 and r = 0.51207, respectively). Furthermore, in the field of Vicenza, a significant negative correlation was found between husk coverage and Fusarium severity (r = −0.41492). Husk coverage emerges as an important trait that merits inclusion in maize breeding programs, given its protective role against fungal infections and its favourable influence on both yield and grain quality.

镰刀菌病以及随之而来的霉菌毒素积累给玉米种植带来了严重问题,其中由疣镰刀菌产生的伏马菌素已成为全球关注的健康问题。为解决这一问题,可采取一系列预防措施(如作物管理技术)来减少真菌感染。应对这一问题的一个有前途的策略是选择对真菌病原体具有更强抵抗力的基因型。这种方法有可能减少对控制真菌生长或间接感染载体的化学投入的依赖。面对气候变化的挑战,利用遗传方法有助于提高农业的经济可持续性。在本研究中,我们评估了两种外皮叶片性状(覆盖率和数量)的重要性、它们与轮纹霉菌感染的关系、伏马菌素含量以及它们对作物产量的潜在影响。研究在意大利北部的三个地方进行,比较了 38 种对轮纹霉菌具有不同抗性的杂交种。研究结果表明,谷壳覆盖率不仅在保护玉米穗免受镰刀菌感染方面起着关键作用,而且对作物产量也有显著影响:在所有三个地点,谷壳覆盖率与产量之间都存在显著的正相关关系(r = 0.33185;r = 0.51327 和 r = 0.51207)。此外,在维琴察田间,稻壳覆盖率与镰刀菌严重程度之间存在显著的负相关(r = -0.41492)。谷壳覆盖率是值得纳入玉米育种计划的一个重要性状,因为它对真菌感染具有保护作用,而且对产量和谷物品质都有有利影响。
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引用次数: 0
Effect of agricultural intensification on the genetic diversity of Helicoverpa armigera 农业集约化对 Helicoverpa armigera 遗传多样性的影响
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-12 DOI: 10.1002/fes3.525
Hongying Cui, Zhaoke Dong, Yingying Song, Wenxiu Guo, Yi Yu, Lili Li, Suhong Lv, Xingyuan Men

Agricultural intensification affects natural and crop ecosystems, and increases the risk of agricultural pests in agroecosystems. Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) is an important pest that damages a wide range of crops. However, the effect of agricultural intensification on the genetic diversity of this pest is still unclear. In this study, we investigated the effects of the composition and configuration of the landscape on the genetic diversity of the agricultural pest H. armigera based on cytochrome oxidase subunit I (COI) analyses. In total, 10 haplotypes were found in 2016 and 15 haplotypes in 2021 based on COI genes. The haplotype diversity and nucleotide diversity were the highest in the Anqiu (AQ) region during 2016 and in the Bincheng (BC) region during 2021. Haplotype 2 and haplotype 3 (Hap2 and Hap3) were the dominant haplotypes in the H. armigera population. Agricultural intensification had no effect on the genetic diversity of H. armigera between 2016 and 2021. Our study highlights the effect of agricultural intensification on the genetic diversity of H. armigera. Understanding the genetic consequences of agricultural intensification is essential for the green control of agricultural pests and the sustainable development of agriculture.

农业集约化会影响自然和作物生态系统,并增加农业生态系统中农业害虫的风险。Helicoverpa armigera Hübner(鳞翅目:夜蛾科)是一种危害多种作物的重要害虫。然而,农业集约化对该害虫遗传多样性的影响仍不清楚。在本研究中,我们基于细胞色素氧化酶亚单位 I(COI)分析,研究了景观的组成和配置对农业害虫 H. armigera 遗传多样性的影响。基于 COI 基因,2016 年共发现 10 个单倍型,2021 年发现 15 个单倍型。2016年安丘(AQ)地区的单倍型多样性和核苷酸多样性最高,2021年滨城(BC)地区的单倍型多样性和核苷酸多样性最高。单倍型2和单倍型3(Hap2和Hap3)是H. armigera种群的优势单倍型。在 2016 年至 2021 年期间,农业集约化对 H. armigera 的遗传多样性没有影响。我们的研究强调了农业集约化对 H. armigera 遗传多样性的影响。了解农业集约化的遗传后果对于农业害虫的绿色防控和农业的可持续发展至关重要。
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引用次数: 0
Building forests for the future 为未来建设森林
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-06 DOI: 10.1002/fes3.518
A. Robert MacKenzie, Sami Ullah, Christine H. Foyer

Many governments have set ambitious targets for tree planting and increased woodland cover as a key part of actions to reach net-zero carbon emissions by 2050. However, many uncertainties remain concerning how and where to expand tree cover, what species to plant, and how best to manage new plantations. Much contemporary forestry has been based on even-aged monocultures, largely because of perceived advantages for timber production. However, in order to play a key role in climate change mitigation future forests will have to achieve timber production (and wider ecosystem service provision) alongside resilience to biotic and abiotic challenge. It is therefore crucial that appropriate informed decisions are made with regard to the structure, composition, and planning of future forests, in order to provide sustainable solutions that provide environmental, economic, and health benefits to society. Genetically diverse, mixed, and irregular forests, with their higher biodiversity and niche complementarity, are promising new forest configurations for regulating the water cycle, storing carbon, and delivering other goods and services. In the following discussion, we have used UK information to illustrate the benefits of mixed woodland versus monocultures and highlighted current issues related to government initiatives and policies for current and future forests. However, similar issues and problems are encountered globally.

作为到 2050 年实现碳净零排放行动的重要组成部分,许多国家的政府都制定了植树造林和增加林地覆盖率的宏伟目标。然而,在如何扩大树木覆盖面积、在何处扩大树木覆盖面积、种植什么树种以及如何最好地管理新的植树造林方面,仍然存在许多不确定因素。当代林业大多以均匀树龄的单一树种为基础,这主要是因为人们认为单一树种在木材生产方面具有优势。然而,为了在减缓气候变化方面发挥关键作用,未来的森林在实现木材生产(以及提供更广泛的生态系统服务)的同时,还必须具备抵御生物和非生物挑战的能力。因此,就未来森林的结构、组成和规划做出适当的知情决策至关重要,以便提供可持续的解决方案,为社会带来环境、经济和健康方面的益处。基因多样化、混交和不规则森林具有更高的生物多样性和生态位互补性,是调节水循环、储存碳和提供其他产品和服务的有前途的新森林配置。在下面的讨论中,我们利用英国的资料来说明混交林地与单一林地的好处,并强调了与政府对当前和未来森林的倡议和政策有关的当前问题。然而,全球都会遇到类似的问题。
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引用次数: 0
Impact of urban productive safety net programme on poverty reduction and food security: Evidence from major cities of Eastern Ethiopia (Dire Dawa, Harar and Jigjiga) 城市生产安全网计划对减贫和粮食安全的影响:埃塞俄比亚东部主要城市(德雷达瓦、哈拉尔和吉吉加)的证据
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-04 DOI: 10.1002/fes3.535
Salah Mohammed Abdulahi, Habtamu Legese Feyisa, Dereje Degu Ayen

Different social protection programs are designed in low-income countries to eradicate poverty and improve the food security of poor people. Currently, the Urban Productive Safety Net Program (UPSNP) is designed to support those who are living in poverty and face food insecurity with predictable and reliable support through food, cash, or vouchers. However, limited empirical evidence has been presented about the significant impact of the program on the well-being of households who participated in the program and the factors that affect the households' decision to participate in the program. Hence, this study aims to evaluate the impact of participation in UPSNP on well-being using household survey data gathered from three main cities of Ethiopia: Dire Dawa, Harar, and Jigjiga in 2022. This study employed both propensity score matching (PSM) and endogenous switching regression (ESR) models to assess the impact of the UPSNP. The result shows that the probability of a household's participation decision is determined by the age of the household head, number of children, savings, house ownership, employment status of the household head, and shock. Furthermore, we found a consistently positive impact across models, indicating that participation in UPSNP reduces poverty and increases food security of households.

低收入国家制定了不同的社会保护计划,以消除贫困和改善贫困人口的食品安全。目前,"城市生产安全网计划"(UPSNP)旨在通过粮食、现金或代金券等方式,为那些生活贫困且面临粮食不安全问题的人提供可预测的可靠支持。然而,关于该计划对参与该计划的家庭福祉的重大影响,以及影响家庭决定参与该计划的因素,所提供的经验证据十分有限。因此,本研究旨在利用从埃塞俄比亚三个主要城市收集到的家庭调查数据,评估参与 UPSNP 对福利的影响:本研究采用了倾向性得分匹配和倾向性得分匹配两种方法。本研究采用倾向得分匹配(PSM)和内生转换回归(ESR)模型来评估 UPSNP 的影响。结果表明,户主年龄、子女数量、储蓄、房屋所有权、户主就业状况和冲击决定了家庭参与决策的概率。此外,我们还发现,不同的模型都产生了一致的积极影响,表明参与《国家粮食保障计划》可以减少贫困,提高家庭的粮食安全。
{"title":"Impact of urban productive safety net programme on poverty reduction and food security: Evidence from major cities of Eastern Ethiopia (Dire Dawa, Harar and Jigjiga)","authors":"Salah Mohammed Abdulahi,&nbsp;Habtamu Legese Feyisa,&nbsp;Dereje Degu Ayen","doi":"10.1002/fes3.535","DOIUrl":"https://doi.org/10.1002/fes3.535","url":null,"abstract":"<p>Different social protection programs are designed in low-income countries to eradicate poverty and improve the food security of poor people. Currently, the Urban Productive Safety Net Program (UPSNP) is designed to support those who are living in poverty and face food insecurity with predictable and reliable support through food, cash, or vouchers. However, limited empirical evidence has been presented about the significant impact of the program on the well-being of households who participated in the program and the factors that affect the households' decision to participate in the program. Hence, this study aims to evaluate the impact of participation in UPSNP on well-being using household survey data gathered from three main cities of Ethiopia: Dire Dawa, Harar, and Jigjiga in 2022. This study employed both propensity score matching (PSM) and endogenous switching regression (ESR) models to assess the impact of the UPSNP. The result shows that the probability of a household's participation decision is determined by the age of the household head, number of children, savings, house ownership, employment status of the household head, and shock. Furthermore, we found a consistently positive impact across models, indicating that participation in UPSNP reduces poverty and increases food security of households.</p>","PeriodicalId":54283,"journal":{"name":"Food and Energy Security","volume":"13 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fes3.535","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140024763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Beyond hunger: Unveiling the rights to food in sub-Saharan Africa 超越饥饿:揭示撒哈拉以南非洲的食物权
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-14 DOI: 10.1002/fes3.530
Helen Onyeaka, Adedola S. Adeboye, Oluwaseun P. Bamidele, Isiguzoro Onyeoziri, Oluwafemi A. Adebo, Maria M. Adeyemi, Susan N. Thera-Sekgweng

This article explores the fundamental right to food, examining its legal framework, its relationship with other human rights, and the efforts being made globally to improve this right. It begins by looking at international and national laws that protect the right to food and examining their importance in ensuring food security and sovereignty. It then explores how food rights intersect with other rights, such as health, education, and labor, revealing the interrelated web of human rights. The article further highlights how the right to food is closely tied to the objectives of reducing poverty and promoting social justice, which are crucial components in the collective effort to achieve food security for all. Additionally, it evaluates the effectiveness of current policies and strategies in promoting the right to food, particularly in sub-Saharan Africa. The novelty of the article is that it brings into perspective, the encumbrance of food sovereignty in sub-Saharan Africa and concludes with potential solutions.

本文探讨基本食物权,研究其法律框架、与其他人权的关系以及全球为改善这一权利所做的努力。文章首先介绍了保护食物权的国际和国内法律,并探讨了这些法律在确保粮食安全和主权方面的重要性。然后,文章探讨了食物权如何与健康权、受教育权和劳动权等其他权利相互交叉,揭示了人权之间相互关联的网络。文章进一步强调了食物权是如何与减少贫困和促进社会公正的目标紧密联系在一起的,这些目标是实现人人享有粮食安全的集体努力的重要组成部分。此外,文章还评估了促进食物权的现行政策和战略的有效性,特别是在撒哈拉以南非洲地区。这篇文章的新颖之处在于,它透视了撒哈拉以南非洲粮食主权的障碍,并在最后提出了潜在的解决方案。
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引用次数: 0
The regulatory landscape of genetically engineered crops in Pakistan 巴基斯坦对转基因作物的监管情况
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-13 DOI: 10.1002/fes3.494
Muhammad Awais, Abdul Qayyum Rao, Sahar Sadaqat, Amina Yaqoob, Muhammad Saad Bhutta, Sana Shakoor, Aneela Yasmeen, Sara Ajmal, Hamed A. Ghramh, Khalid Ali Khan, Allah Bakhsh

Genome editing technology has become part and parcel of biotechnological approach to understand the genetic basis of different biological processes in an organism and for its application in different fields. The basic need of world food production demands a highly variable population of plants with maximum diversity of agronomic traits of interest. Genome editing is unique in the sense that it provides a direct approach to generate targeted variability. The worldwide availability of genome-edited crop varieties in the global market is approaching, whereas the decision to deal with genome-edited (GE) crops differently from genetically transformed crop varieties is under discussion all around the world. GE may add allochthonous or autochthonous sequences into the genome much like traditional transgenesis or cisgenesis. Considering this fact, the United States declared genome-edited crops as conventional. Many countries such as Japan, UK, China, and Brazil have already legislated their GE products and their products are near to get entry to the market. In Pakistan, academic and research-based scientific institutions have been working on genome-edited crops by using CRISPR (clustered regularly interspaced short palindromic repeats) Cas technology. As being a signatory of the Cartagena protocol, every case of GM (Genetically modified) or GE (Genetically Engineered) should be passed through the Technical Advisory Committee which will be decided on case-to-case bases depending upon its nature of random or targeted mutation. Pakistan Environmental Protection Act, Intellectual Property Organization of Pakistan Act, Seed amendment act, and PBR (The Plant Breeders' Rights Act) are the major constitutional acts employed and working for the regulations of GM alongside GE crops. Now, discussions of scientists, academicians and officials from regulatory body are in process for the design of GE crop commercialization policy in Pakistan. The CRISPR-Cas9 system is now the most in-demand piece of technology and has become an indispensable component of research and development in the field of life sciences.

基因组编辑技术已成为生物技术方法的重要组成部分,以了解生物体内不同生物过程的遗传基础,并将其应用于不同领域。世界粮食生产的基本需求要求植物群体具有高度的可变性,并具有最大限度的农艺性状多样性。基因组编辑的独特之处在于,它提供了一种直接生成目标变异性的方法。经过基因组编辑的作物品种即将在全球市场上市,而如何区别对待经过基因组编辑(GE)的作物和经过基因转化的作物品种,则是全世界都在讨论的问题。GE 可能会像传统的转基因或顺基因一样,在基因组中添加同源或自源序列。考虑到这一事实,美国宣布基因组编辑作物为常规作物。许多国家,如日本、英国、中国和巴西,已经对其基因工程产品进行了立法,其产品即将进入市场。在巴基斯坦,以学术和研究为基础的科研机构一直在利用 CRISPR(簇状规则间隔短回文重复序列)Cas 技术研究基因组编辑作物。作为《卡塔赫纳议定书》的签署国,每一例转基因(GM)或 GE(Genetically Engineered)都应通过技术咨询委员会,该委员会将根据随机突变或定向突变的性质逐案做出决定。巴基斯坦环境保护法》、《巴基斯坦知识产权组织法》、《种子修正法》和《植物育种者权利法》是用于管理转基因和基因工程作物的主要宪法法案。目前,科学家、院士和监管机构的官员正在讨论制定巴基斯坦的转基因作物商业化政策。CRISPR-Cas9 系统是目前最炙手可热的技术,已成为生命科学领域研发工作不可或缺的组成部分。
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引用次数: 0
Recruiting grape-isolated antagonistic yeasts for the sustainable bio-management of Botrytis cinerea on grapes 招募葡萄分离拮抗酵母,对葡萄上的灰葡萄孢菌进行可持续生物管理
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-09 DOI: 10.1002/fes3.528
Sebahat Oztekin, Funda Karbancioglu-Guler

Botrytis cinerea is the causative agent of grey mould disease in grapes, which was linked to significant postharvest losses. This study examined three grape-isolated yeasts (Metschnikowia aff. fructicola, Metschnikowia pulcherrima, and Hansenispora uvarum) through in vitro and in vivo tests on detached grape berries against grey mould, as well as the elucidation of their possible mechanisms of action. The antifungal mechanism of action of yeasts was determined by the lytic enzyme activity, inhibition of spore germination, biofilm activity, iron depletion, diffusible metabolites, wound-site colonisation, mycocin, and volatile organic compounds (VOCs) production. The highest in vitro efficacy (83.13%) was observed on M. aff. fructicola, followed by M. pulcherrima (82.10%) and H. uvarum (71.66%). Metschnikowia yeasts exhibited comparable enzyme activities, including protease, β-1,3 glucanase, gelatinase chitinase, and cellulase, while H. uvarum had a poor enzymatic activity with chitinase and gelatinase. M. aff. fructicola showed relatively higher iron depletion activity than M. pulcherrima, while M. pulcherrima outperformed via diffusible metabolites. All yeast cultures significantly reduced spore germination by at least 86%. Overall, M. aff. fructicola exhibited the highest biocontrol activity with its iron depletion, inhibition of conidial germination, biofilm formation, VOCs, and well colonisation on grape berries. M. aff. fructicola 1-UDM outperformed all other yeasts by significantly reducing disease incidence and lesion diameter values (93.4% and 94.3%, respectively). Remarkably, H. uvarum VOCs demonstrated potential as a biofumigant for suppressing grey mould. All yeasts are well adapted to their ecological niche to bio-protect grapes from grey mould disease.

灰葡萄孢菌是葡萄灰霉病的致病菌,与葡萄采后的重大损失有关。本研究通过对分离的葡萄果实进行抗灰霉病的体内外试验,研究了三种葡萄分离酵母菌(Metschnikowia aff. fructicola、Metschnikowia pulcherrima 和 Hansenispora uvarum),并阐明了它们可能的作用机制。酵母菌的抗真菌作用机制是通过裂解酶活性、孢子萌发抑制、生物膜活性、铁耗竭、可扩散代谢物、伤口定植、霉菌素和挥发性有机化合物(VOCs)的产生来确定的。对 M. aff. fructicola 的体外效力最高(83.13%),其次是 M. pulcherrima(82.10%)和 H. uvarum(71.66%)。Metschnikowia 酵母菌的酶活性相当,包括蛋白酶、β-1,3 葡聚糖酶、明胶酶、几丁质酶和纤维素酶,而 H. uvarum 的几丁质酶和明胶酶活性较差。M. aff. fructicola 比 M. pulcherrima 表现出更高的铁耗竭活性,而 M. pulcherrima 通过可扩散代谢物表现更佳。所有酵母培养物都能明显减少至少 86% 的孢子萌发。总体而言,果蝇酵母菌的生物控制活性最高,它能消耗铁、抑制分生孢子萌发、生物膜形成、挥发性有机化合物以及在葡萄果实上的良好定殖。M. aff. fructicola 1-UDM 的表现优于所有其他酵母菌,可显著降低病害发生率和病害直径值(分别为 93.4% 和 94.3%)。值得注意的是,H. uvarum VOCs 具有作为生物熏蒸剂抑制灰霉病的潜力。所有酵母菌都能很好地适应其生态位,为葡萄灰霉病提供生物保护。
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引用次数: 0
Impact of salicylic acid and sodium hydrosulfide applied singly or in combination on drought tolerance and grain yield in wheat plants 单独或联合使用水杨酸和硫氢化钠对小麦植物耐旱性和谷物产量的影响
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-09 DOI: 10.1002/fes3.532
Sabri Akin, Cengiz Kaya

Climate change-induced drought stress (DS) poses a significant threat to crop production, emphasizing the need for innovative strategies to mitigate its adverse effects. Prior studies have demonstrated the distinct capacities of salicylic acid (SA) and sodium hydrosulfide (NaHS) to augment plant resilience against drought-related stressors. However, little is known about how they work together or the specific processes by which they increase DS tolerance. The purpose of this research was to determine how SA and NaHS affected the performance of wheat plants during the growing seasons of 2021–2022 and 2022–2023, when there was a drought. The research employed a block-randomized experimental layout with split plots, where the primary factors included two irrigation levels: full irrigation (IW1, 100% of water requirement) and deficit irrigation (IW2, 50% of water requirement). Secondary factors included the application of mock control, 0.5 mM SA, and 0.3 mM NaHS, an H2S donor, either individually or in combination, administered before the onset of DS. The application of SA, NaHS, or their combination significantly enhanced wheat plant resistance to DS. Significant increases in a number of physiological markers, including proline content, relative water content (RWC), Fv/Fm, chlorophyll content, and antioxidant enzyme activity, demonstrated this improvement. Furthermore, in drought-stressed wheat plants, SA and NaHS treatments decreased the amounts of hydrogen peroxide (H2O2), malondialdehyde (MDA) content, and electrolyte leakage (EL). In conclusion, our study highlights the possibility of SA and NaHS, whether applied individually or in combination, to improve drought resistance in wheat plants, presenting a viable approach to lessen the effects of climate change on agricultural yield.

气候变化引起的干旱胁迫(DS)对作物生产构成了重大威胁,因此需要采取创新战略来减轻其不利影响。先前的研究已经证明,水杨酸(SA)和硫氢化钠(NaHS)具有不同的能力来增强植物对干旱相关胁迫的恢复力。然而,人们对这两种物质如何协同作用或它们提高 DS 耐受力的具体过程知之甚少。本研究旨在确定 SA 和 NaHS 在 2021-2022 年和 2022-2023 年干旱生长季节如何影响小麦植株的表现。研究采用了分块随机试验布局,主要因素包括两种灌溉水平:充分灌溉(IW1,需水量的 100%)和亏缺灌溉(IW2,需水量的 50%)。次要因素包括在 DS 开始前单独或联合施用模拟对照、0.5 mM SA 和 0.3 mM NaHS(一种 H2S 供体)。施用 SA、NaHS 或它们的组合能显著增强小麦植株对 DS 的抗性。脯氨酸含量、相对含水量 (RWC)、Fv/Fm、叶绿素含量和抗氧化酶活性等一系列生理指标的显著增加证明了这种改善。此外,在干旱胁迫的小麦植株中,SA 和 NaHS 处理降低了过氧化氢(H2O2)含量、丙二醛(MDA)含量和电解质渗漏(EL)。总之,我们的研究强调了单独或联合应用 SA 和 NaHS 提高小麦植株抗旱性的可能性,为减轻气候变化对农业产量的影响提供了一种可行的方法。
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引用次数: 0
Quantitative trait locus (QTL) mapping and transcriptome profiling identify QTLs and candidate genes associated with heat stress response during reproductive development in Camelina sativa 定量性状位点(QTL)绘图和转录组分析确定了与荠菜生殖发育过程中热胁迫响应相关的 QTL 和候选基因
IF 5 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-09 DOI: 10.1002/fes3.531
Brian E. Smith, Sunny Kemmer, Samuel Decker, Chaofu Lu

Camelina sativa (L.) Crantz is a low-input oilseed crop that has great potential in providing sustainable feedstock for biofuels and bioproducts. Climate change is threatening production of camelina with rising global temperatures. Elucidating the genetic response to high temperatures is essential for successful breeding of heat-tolerant camelina varieties. Here, we report a combinatorial approach to identifying candidate genes associated with heat stress by quantitative trait locus (QTL) mapping and comparative transcriptome profiling. A population of recombinant inbred lines (RILs) was grown in a controlled growth chamber under the high-temperature regimes for 14 days beginning at the onset of the reproductive stage. Several traits related to seed production were evaluated at maturity. The QTL analysis identified several regions with co-located traits on chromosomes 8, 10, and 12. Two RILs with contrasting phenotypic responses to heat stress were chosen for gene expression profiling via RNA sequencing. Multiple pathways and genes were found to be strongly affected by heat stress, and many genes expressed differently between the two RILs. Several genes identified within the QTL regions were considered strong candidates that may control heat tolerance during reproduction in camelina. These studies provide resources for future studies that may assist in improving the heat tolerance of camelina.

荠菜(L. Camelina sativa (L.) Crantz)是一种低投入的油籽作物,在为生物燃料和生物产品提供可持续原料方面具有巨大潜力。随着全球气温的不断升高,气候变化正威胁着荠菜的生产。阐明基因对高温的反应对于成功培育耐热荠菜品种至关重要。在此,我们报告了一种通过定量性状位点(QTL)绘图和比较转录组图谱确定与热胁迫相关的候选基因的组合方法。重组近交系(RIL)群体从生殖期开始在高温条件下在受控生长室中生长了 14 天。在成熟期对与种子生产相关的几个性状进行了评估。QTL 分析确定了第 8、10 和 12 号染色体上具有共定位性状的几个区域。通过 RNA 测序,选择了两个对热胁迫的表型反应截然不同的 RIL 进行基因表达谱分析。研究发现,多种途径和基因受到热胁迫的强烈影响,许多基因在两个 RIL 之间表达不同。在 QTL 区域内发现的几个基因被认为是可能控制荠菜繁殖期间耐热性的有力候选基因。这些研究为今后的研究提供了资源,可能有助于提高荠菜的耐热性。
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Food and Energy Security
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