Pub Date : 2023-05-18DOI: 10.3897/neobiota.84.104682
H. Jactel, A. Battisti, M. Branco, J. Douma, M. Kenis, C. Orazio, C. Robinet, A. Santini, Anna Sapundzhieva, M. L. Seehausen, P. Stoev
{"title":"Management options for non-native forest pests along their invasion pathways","authors":"H. Jactel, A. Battisti, M. Branco, J. Douma, M. Kenis, C. Orazio, C. Robinet, A. Santini, Anna Sapundzhieva, M. L. Seehausen, P. Stoev","doi":"10.3897/neobiota.84.104682","DOIUrl":"https://doi.org/10.3897/neobiota.84.104682","url":null,"abstract":"<jats:p />","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"1 1","pages":""},"PeriodicalIF":5.1,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41656926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.3897/neobiota.84.95177
F. Colombari, A. Battisti
The serious and growing threat posed by biological invasions to biodiversity and livelihoods means that public engagement in dealing with problems of invasive alien species is ever more urgent and necessary hence a citizen science experiment was carried out in north-eastern Italy. The study aimed i) to raise awareness of invasive alien species threatening trees and forests, and ii) to perform monitoring activities of a group of wood borers as an example, involving teachers and students (aged 11 to 18) of high schools. Students and teachers were given background knowledge and trained about the protocol for collecting data in schoolyards/grounds. Native (Anisandrus dispar (Fabricius, 1792), Xyleborinus saxesenii (Ratzeburg, 1837)) and exotic ambrosia beetles (Anisandrus maiche Kurentsov, Cnestus mutilatus (Blandford), Xylosandrus crassiusculus (Motschulsky, 1866), Xylosandrus germanus (Blandford, 1894)) were used as the target species, as they could be easily detected through accessible and low-cost traps requiring limited effort. The traps were exposed for 24 hours weekly between March and June 2021. The experiment also aimed to strengthen public involvement, connecting environmental education and experiential outdoor learning. The mutual collaboration resulted in a wider knowledge of the potential impact of exotic species. It also led to new geographical citizen-science records of two alien ambrosia beetles considered to be quarantine pests by the European Union: C. mutilatus, new for the European part of the EPPO (European and Mediterranean Plant Protection Organization) region, and A. maiche, which was previously found only in Eastern European EPPO member countries.
{"title":"Citizen science at school increases awareness of biological invasions and contributes to the detection of exotic ambrosia beetles","authors":"F. Colombari, A. Battisti","doi":"10.3897/neobiota.84.95177","DOIUrl":"https://doi.org/10.3897/neobiota.84.95177","url":null,"abstract":"The serious and growing threat posed by biological invasions to biodiversity and livelihoods means that public engagement in dealing with problems of invasive alien species is ever more urgent and necessary hence a citizen science experiment was carried out in north-eastern Italy. The study aimed i) to raise awareness of invasive alien species threatening trees and forests, and ii) to perform monitoring activities of a group of wood borers as an example, involving teachers and students (aged 11 to 18) of high schools. Students and teachers were given background knowledge and trained about the protocol for collecting data in schoolyards/grounds. Native (Anisandrus dispar (Fabricius, 1792), Xyleborinus saxesenii (Ratzeburg, 1837)) and exotic ambrosia beetles (Anisandrus maiche Kurentsov, Cnestus mutilatus (Blandford), Xylosandrus crassiusculus (Motschulsky, 1866), Xylosandrus germanus (Blandford, 1894)) were used as the target species, as they could be easily detected through accessible and low-cost traps requiring limited effort. The traps were exposed for 24 hours weekly between March and June 2021. The experiment also aimed to strengthen public involvement, connecting environmental education and experiential outdoor learning. The mutual collaboration resulted in a wider knowledge of the potential impact of exotic species. It also led to new geographical citizen-science records of two alien ambrosia beetles considered to be quarantine pests by the European Union: C. mutilatus, new for the European part of the EPPO (European and Mediterranean Plant Protection Organization) region, and A. maiche, which was previously found only in Eastern European EPPO member countries.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41753954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.3897/neobiota.84.90843
A. Pepori, N. Luchi, F. Pecori, A. Santini
Dutch elm disease (DED) is a destructive tracheomycosis caused by Ophiostoma novo-ulmi, an ascomycete probably originating in East-Asia that is devastating natural elm populations throughout Europe, North America and Asia. The fungus is mainly spread by elm bark beetles that complete their life cycle between healthy and diseased elms. Recently, it has been highlighted that some fungi of the genus Geosmithia, which are similarly well associated with bark beetles, seem to also play a role in the DED pathosystem acting as mycoparasites of O. novo-ulmi. Although some relationship between the fungi is clear, the biological cycle of Geosmithia spp. within the DED cycle is still partly unclear, as is the role of Geosmithia spp. in association with the bark beetles. In this work, we tried to clarify these aspects by developing a qPCR duplex TaqMan assay to detect and quantify DNA of both fungi. The assay is extremely sensitive showing a limit of detection as low as 2 fg μl–1 for both fungi. We collected woody samples from healthy and infected elm trees throughout the beetle life cycle. All healthy elm samples were negative for both Geosmithia spp. and O. novo-ulmi DNA. Geosmithia spp. are never present in infected, but living trees, while they are present in frass of elm bark beetles (EBB – Scolytus spp.) and at each stage of the EBB life cycle in much higher quantities than O. novo-ulmi. This work provides a better understanding of the role and interactions occurring amongst the main players of the DED pathosystem.
{"title":"Duplex real-time PCR assay for the simultaneous detection of Ophiostoma novo-ulmi and Geosmithia spp. in elm wood and insect vectors","authors":"A. Pepori, N. Luchi, F. Pecori, A. Santini","doi":"10.3897/neobiota.84.90843","DOIUrl":"https://doi.org/10.3897/neobiota.84.90843","url":null,"abstract":"Dutch elm disease (DED) is a destructive tracheomycosis caused by Ophiostoma novo-ulmi, an ascomycete probably originating in East-Asia that is devastating natural elm populations throughout Europe, North America and Asia. The fungus is mainly spread by elm bark beetles that complete their life cycle between healthy and diseased elms. Recently, it has been highlighted that some fungi of the genus Geosmithia, which are similarly well associated with bark beetles, seem to also play a role in the DED pathosystem acting as mycoparasites of O. novo-ulmi. Although some relationship between the fungi is clear, the biological cycle of Geosmithia spp. within the DED cycle is still partly unclear, as is the role of Geosmithia spp. in association with the bark beetles. In this work, we tried to clarify these aspects by developing a qPCR duplex TaqMan assay to detect and quantify DNA of both fungi. The assay is extremely sensitive showing a limit of detection as low as 2 fg μl–1 for both fungi. We collected woody samples from healthy and infected elm trees throughout the beetle life cycle. All healthy elm samples were negative for both Geosmithia spp. and O. novo-ulmi DNA. Geosmithia spp. are never present in infected, but living trees, while they are present in frass of elm bark beetles (EBB – Scolytus spp.) and at each stage of the EBB life cycle in much higher quantities than O. novo-ulmi. This work provides a better understanding of the role and interactions occurring amongst the main players of the DED pathosystem.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46048368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.3897/neobiota.84.90024
Noemi Casarin, Séverine Hasbroucq, Júlia López-Mercadal, Miguel Ángel Miranda, Claude Bragard, Jean-Claude Grégoire
The sentinel plantation concept consists of assessing the impact of exotic factors, such as pests and pathogens, on plants of interest by planting them out of their native range. This tool is a way to enhance knowledge for pest risk analysis (PRA) by guiding decisions on how quarantine organisms should be regulated and where to focus prevention and surveillance efforts for an early detection. In this study, the sentinel method was used in the case of research on Xylella fastidiosa , a plant pathogenic bacterium that has recently been found established in southern Europe, but whose potential impact and possible host range are still poorly documented in northern areas where the bacterium is not known to occur. To improve knowledge on the susceptibility of potential hosts of X. fastidiosa in northern Europe, a sentinel plantation of Prunus domestica cv. Opal, Quercus petraea and Salix alba was established in the X. fastidiosa -infected area of Majorca. In order to assess the circulation of the bacterium in the sentinel plot and around it, surveys of the local flora and insect vectors were carried out, as well as the planting of a network of rosemary “spy plants”. Symptomatic monitoring and molecular analyses were performed on the sentinel plants for four years. During these years, X. fastidiosa was never detected in our sentinel plants most likely because of the low infectivity pressure recorded in the surroundings. This study underlines the complexity of conducting sentinel plantation assays combined with X. fastidiosa research, highlighting the need for long-term investigation and questioning the efficiency of the sentinel tool. However, this study is placed in perspective with other valuable sentinel plantations. It also highlights the complementarity of the tool and proposes elements to improve or re-orientate the implementation of future sentinel projects.
{"title":"Measuring the threat from a distance: insight into the complexity and perspectives for implementing sentinel plantation to test the host range of Xylella fastidiosa","authors":"Noemi Casarin, Séverine Hasbroucq, Júlia López-Mercadal, Miguel Ángel Miranda, Claude Bragard, Jean-Claude Grégoire","doi":"10.3897/neobiota.84.90024","DOIUrl":"https://doi.org/10.3897/neobiota.84.90024","url":null,"abstract":"The sentinel plantation concept consists of assessing the impact of exotic factors, such as pests and pathogens, on plants of interest by planting them out of their native range. This tool is a way to enhance knowledge for pest risk analysis (PRA) by guiding decisions on how quarantine organisms should be regulated and where to focus prevention and surveillance efforts for an early detection. In this study, the sentinel method was used in the case of research on Xylella fastidiosa , a plant pathogenic bacterium that has recently been found established in southern Europe, but whose potential impact and possible host range are still poorly documented in northern areas where the bacterium is not known to occur. To improve knowledge on the susceptibility of potential hosts of X. fastidiosa in northern Europe, a sentinel plantation of Prunus domestica cv. Opal, Quercus petraea and Salix alba was established in the X. fastidiosa -infected area of Majorca. In order to assess the circulation of the bacterium in the sentinel plot and around it, surveys of the local flora and insect vectors were carried out, as well as the planting of a network of rosemary “spy plants”. Symptomatic monitoring and molecular analyses were performed on the sentinel plants for four years. During these years, X. fastidiosa was never detected in our sentinel plants most likely because of the low infectivity pressure recorded in the surroundings. This study underlines the complexity of conducting sentinel plantation assays combined with X. fastidiosa research, highlighting the need for long-term investigation and questioning the efficiency of the sentinel tool. However, this study is placed in perspective with other valuable sentinel plantations. It also highlights the complementarity of the tool and proposes elements to improve or re-orientate the implementation of future sentinel projects.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135674393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.3897/neobiota.84.90981
M. Dvořák, Petr Štoidl, M. Rost
Currently, the ash dieback causal agent Hymenoscyphus fraxineus is an established invasive pathogen in most European countries. Its potential to spread quickly among invaded forests is based on its propagules: airborne inoculum composed mainly of ascospores originated in apothecia growing on leaf litter infected during the previous vegetation season. The spread of the inoculum by air masses to distant areas is probable and depends on the availability of the ascospores in higher levels of air. Our study aimed to detect the inoculum in an infected area at heights of more than 20 meters. Our study was conducted in a municipal locality (Boršov nad Vltavou) with tens of infected ash trees (Fraxinus excelsior) in South Bohemia (SW Czechia). The infected trees surround an agricultural silo where five rotating arm spore traps (rotorods) were mounted for ten consequent 48h samplings during the peak of the sporulating season (17th July to 6th August 2020). The spore traps were mounted 48, 37, 25, 14 and 0,3 meters above ground. Samples were quantified by qPCR. Results clearly proved the ability of the spores to reach a height of 48 meters. Furthermore, H. fraxineus DNA was detected from all five spore traps during all ten samplings. Mostly, the amount of detected spores showed a decreasing trend with height, and varied a lot. During some of the samplings, higher spore concetrations were achieved at the top than at the lower traps, which can be explained by horizontal air transfer of the inoculum from other infected areas. Based on GLM analyses, higher spore concentrations were achieved during days without rain, lower air temperatures, after cloudy, humid and rainy weather without strong winds. A combination of rotorod ROTTRAP 52 with qPCR quantification proved to be an efficient technology for a study focused on the vertical spread of H. fraxineus propagules.
目前，灰死病菌是大多数欧洲国家公认的入侵病原体。它在被入侵的森林中迅速传播的潜力是基于它的繁殖体：空气传播的接种物主要由子囊孢子组成，子囊孢子起源于生长在前一个植被季节感染的落叶上的古猿。接种物通过气团传播到远处是可能的，这取决于子囊孢子在较高水平空气中的可用性。我们的研究旨在检测20米以上高度感染区的接种物。我们的研究是在南波希米亚（捷克西南部）的一个市政地区（Boršov nad Vltavou）进行的，该地区有数十棵受感染的白蜡树（Fraxinus excelsior）。受感染的树木围绕着一个农业筒仓，在孢子形成季节的高峰期（2020年7月17日至8月6日），在那里安装了五个旋转臂孢子捕捉器（旋转棒），随后进行了10次48小时的采样。孢子捕集器分别安装在离地48、37、25、14和0.3米处。通过qPCR对样品进行定量。结果清楚地证明了孢子达到48米高度的能力。此外，在所有10次采样中，从所有5个孢子陷阱中都检测到了黄曲霉的DNA。大多数情况下，检测到的孢子数量随着高度的增加呈下降趋势，并且变化很大。在一些采样过程中，顶部的孢子浓度高于下部的捕集器，这可以通过接种物从其他感染区域的水平空气转移来解释。根据GLM分析，在无雨、气温较低的日子里，在多云、潮湿和下雨且无强风的天气之后，孢子浓度较高。rotorod ROTTRAP 52与qPCR定量的结合被证明是一种有效的技术，用于研究黄曲霉繁殖体的垂直传播。
{"title":"Vertical spread of Hymenoscyphus fraxineus propagules","authors":"M. Dvořák, Petr Štoidl, M. Rost","doi":"10.3897/neobiota.84.90981","DOIUrl":"https://doi.org/10.3897/neobiota.84.90981","url":null,"abstract":"Currently, the ash dieback causal agent Hymenoscyphus fraxineus is an established invasive pathogen in most European countries. Its potential to spread quickly among invaded forests is based on its propagules: airborne inoculum composed mainly of ascospores originated in apothecia growing on leaf litter infected during the previous vegetation season. The spread of the inoculum by air masses to distant areas is probable and depends on the availability of the ascospores in higher levels of air. Our study aimed to detect the inoculum in an infected area at heights of more than 20 meters. Our study was conducted in a municipal locality (Boršov nad Vltavou) with tens of infected ash trees (Fraxinus excelsior) in South Bohemia (SW Czechia). The infected trees surround an agricultural silo where five rotating arm spore traps (rotorods) were mounted for ten consequent 48h samplings during the peak of the sporulating season (17th July to 6th August 2020). The spore traps were mounted 48, 37, 25, 14 and 0,3 meters above ground. Samples were quantified by qPCR. Results clearly proved the ability of the spores to reach a height of 48 meters. Furthermore, H. fraxineus DNA was detected from all five spore traps during all ten samplings. Mostly, the amount of detected spores showed a decreasing trend with height, and varied a lot. During some of the samplings, higher spore concetrations were achieved at the top than at the lower traps, which can be explained by horizontal air transfer of the inoculum from other infected areas. Based on GLM analyses, higher spore concentrations were achieved during days without rain, lower air temperatures, after cloudy, humid and rainy weather without strong winds. A combination of rotorod ROTTRAP 52 with qPCR quantification proved to be an efficient technology for a study focused on the vertical spread of H. fraxineus propagules.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44681606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.3897/neobiota.84.95761
Samantha Green, Katharina Dehnen-Schmutz, Jassy Drakulic, René Eschen, Christophe Orazio, Jacob C. Douma, Karl Lundén, Fernanda Colombari, Hervé Jactel
Emerging and invasive tree pests and pathogens in Europe are increasing in number and range, having impacts on biodiversity, forest services, ecosystems and human well-being. Stakeholders involved in tree and forest management contribute to the detection and management of new and emerging tree pests and pathogens (PnPs). We surveyed different groups of stakeholders in European countries. The stakeholders were mainly researchers, tree health surveyors and forest managers, as well as forest owners, nurseries, policy-makers, advisors, forestry authorities, NGOs and civil society. We investigated which tools they used to detect and manage PnPs, surveyed their current PnP awareness and knowledge and collated the new and emerging PnP species of concern to them. The 237 respondents were based in 15 European countries, with the majority from the United Kingdom, France and the Czech Republic. There was a strong participation of respondents with a work focus on research and surveying, whereas timber traders and plant importers were less represented. Respondents were surveyed on 18 new, emerging PnPs in Europe and listed an additional 37 pest species and 21 pathogen species as potential future threats. We found that species on EPPO’s list of ‘priority pests’ were better known than those not listed. Stakeholders working in urban environments were more aware of PnPs compared to those working in rural areas. Stakeholders’ awareness of PnPs was not related to the number of new, emerging PnP species present in a country. Stakeholders want access to more detection and management tools, including long-term citizen-science monitoring, maps showing spread and range of new PnPs, pest identification smartphone apps, hand-held detection devices, drone monitoring and eDNA metabarcoding. To help facilitate better forest health across Europe, they called for mixed forest development, reduced nursery stock movement, biosecurity and data sharing amongst organisations. These results indicate that stakeholder knowledge of a few key PnP may be good, but given that the large diversity of threats is so large and future risks unknown, we conclude that multiple and varied methods for generic detection, mitigation and management methods, many in development, are needed in the hands of stakeholders surveying and managing trees and woodlands in Europe.
{"title":"Awareness, detection and management of new and emerging tree pests and pathogens in Europe: stakeholders’ perspectives","authors":"Samantha Green, Katharina Dehnen-Schmutz, Jassy Drakulic, René Eschen, Christophe Orazio, Jacob C. Douma, Karl Lundén, Fernanda Colombari, Hervé Jactel","doi":"10.3897/neobiota.84.95761","DOIUrl":"https://doi.org/10.3897/neobiota.84.95761","url":null,"abstract":"Emerging and invasive tree pests and pathogens in Europe are increasing in number and range, having impacts on biodiversity, forest services, ecosystems and human well-being. Stakeholders involved in tree and forest management contribute to the detection and management of new and emerging tree pests and pathogens (PnPs). We surveyed different groups of stakeholders in European countries. The stakeholders were mainly researchers, tree health surveyors and forest managers, as well as forest owners, nurseries, policy-makers, advisors, forestry authorities, NGOs and civil society. We investigated which tools they used to detect and manage PnPs, surveyed their current PnP awareness and knowledge and collated the new and emerging PnP species of concern to them. The 237 respondents were based in 15 European countries, with the majority from the United Kingdom, France and the Czech Republic. There was a strong participation of respondents with a work focus on research and surveying, whereas timber traders and plant importers were less represented. Respondents were surveyed on 18 new, emerging PnPs in Europe and listed an additional 37 pest species and 21 pathogen species as potential future threats. We found that species on EPPO’s list of ‘priority pests’ were better known than those not listed. Stakeholders working in urban environments were more aware of PnPs compared to those working in rural areas. Stakeholders’ awareness of PnPs was not related to the number of new, emerging PnP species present in a country. Stakeholders want access to more detection and management tools, including long-term citizen-science monitoring, maps showing spread and range of new PnPs, pest identification smartphone apps, hand-held detection devices, drone monitoring and eDNA metabarcoding. To help facilitate better forest health across Europe, they called for mixed forest development, reduced nursery stock movement, biosecurity and data sharing amongst organisations. These results indicate that stakeholder knowledge of a few key PnP may be good, but given that the large diversity of threats is so large and future risks unknown, we conclude that multiple and varied methods for generic detection, mitigation and management methods, many in development, are needed in the hands of stakeholders surveying and managing trees and woodlands in Europe.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135718116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.3897/neobiota.84.95823
T. Paap, A. Santini, C. Rodas, G. Granados, F. Pecori, M. Wingfield
Austropuccinia psidii, the causal agent of myrtle rust, has emerged as a significant threat to Myrtaceae in planted and natural woody ecosystems. The first detection of A. puccinia in South Africa was from severely infected ornamental Myrtus communis. This raised concern that M. communis, the sole Myrtaceae species native to Europe and an important component of vegetation in Mediterranean regions, could be threatened by the rust. In light of the potential threat to this unique species, seed was collected from 12 Italian provenances of M. communis, including mainland and island (Sardinia and Sicily) populations. We assessed the susceptibility of these provenances to both the pandemic and South African strains of A. psidii. In Colombia, where the pandemic strain of A. psidii is native, seedlings rapidly became infected by natural inoculum. In South Africa, a preliminary screening of seedlings by artificial inoculation with a single-uredinium isolate produced high levels of disease. Finally, plants of each of the 12 provenances were planted and monitored in Florence, Italy. To date, these showed no signs of disease, but will continue to be monitored. This study highlights the significant threat that both the pandemic and South African strains of A. puccinia pose to M. communis in Europe.
{"title":"Myrtus communis in Europe threatened by the pandemic and South African strains of the myrtle rust pathogen Austropuccinia psidii (Sphaerophragmiaceae, Pucciniales)","authors":"T. Paap, A. Santini, C. Rodas, G. Granados, F. Pecori, M. Wingfield","doi":"10.3897/neobiota.84.95823","DOIUrl":"https://doi.org/10.3897/neobiota.84.95823","url":null,"abstract":"Austropuccinia psidii, the causal agent of myrtle rust, has emerged as a significant threat to Myrtaceae in planted and natural woody ecosystems. The first detection of A. puccinia in South Africa was from severely infected ornamental Myrtus communis. This raised concern that M. communis, the sole Myrtaceae species native to Europe and an important component of vegetation in Mediterranean regions, could be threatened by the rust. In light of the potential threat to this unique species, seed was collected from 12 Italian provenances of M. communis, including mainland and island (Sardinia and Sicily) populations. We assessed the susceptibility of these provenances to both the pandemic and South African strains of A. psidii. In Colombia, where the pandemic strain of A. psidii is native, seedlings rapidly became infected by natural inoculum. In South Africa, a preliminary screening of seedlings by artificial inoculation with a single-uredinium isolate produced high levels of disease. Finally, plants of each of the 12 provenances were planted and monitored in Florence, Italy. To date, these showed no signs of disease, but will continue to be monitored. This study highlights the significant threat that both the pandemic and South African strains of A. puccinia pose to M. communis in Europe.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49384821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-12DOI: 10.3897/neobiota.83.101416
Chika Egawa, Asuka Koyama
Clarifying the temporal trends of alien plant accumulation is increasingly important for informing global and national management efforts to decelerate biological invasions, following the adoption of Target 6 of the Kunming-Montreal Global Biodiversity Framework. However, such trends have not yet been analysed in many countries including Japan, which has the highest number of naturalised alien plant species among islands. To clarify the past and recent trends in the accumulation of alien plant species in Japan, we compiled a dataset of the year of first record for 1,463 alien vascular plant species deliberately and accidentally introduced and analysed the changes in the annual number of first records over time for each overall, intentional and unintentional introductions. We found that, overall, the annual number of first records of alien plant species in Japan began to increase in the late 1800s, and the increase continued until the late 1950s, with an estimated maximum of 15.7 new species per year. The increase then halted by 1960 and began a slow decline; the estimated average records per year between 1991 and 2000 dropped to 13.3 species. Since 1900, the annual number of first records associated with intentional introductions has more than doubled the number linked to unintentional introductions. Additionally, the proportion of invasive species brought through intentional introductions was larger than that brought through unintentional introductions. We highlight that while Japan experienced a rapid accumulation of alien plant species, including invasive species, by the 1950s, the rate of accumulation showed signs of saturation by 1960 and has since been slowly declining. Further deceleration and prevention of the introduction of invasive alien species, as targeted in the Kunming-Montreal Global Biodiversity Framework, may be achieved through increased investment in pathway management, especially management of intentional pathways.
{"title":"Temporal trends in the accumulation of alien vascular plant species through intentional and unintentional introductions in Japan","authors":"Chika Egawa, Asuka Koyama","doi":"10.3897/neobiota.83.101416","DOIUrl":"https://doi.org/10.3897/neobiota.83.101416","url":null,"abstract":"Clarifying the temporal trends of alien plant accumulation is increasingly important for informing global and national management efforts to decelerate biological invasions, following the adoption of Target 6 of the Kunming-Montreal Global Biodiversity Framework. However, such trends have not yet been analysed in many countries including Japan, which has the highest number of naturalised alien plant species among islands. To clarify the past and recent trends in the accumulation of alien plant species in Japan, we compiled a dataset of the year of first record for 1,463 alien vascular plant species deliberately and accidentally introduced and analysed the changes in the annual number of first records over time for each overall, intentional and unintentional introductions. We found that, overall, the annual number of first records of alien plant species in Japan began to increase in the late 1800s, and the increase continued until the late 1950s, with an estimated maximum of 15.7 new species per year. The increase then halted by 1960 and began a slow decline; the estimated average records per year between 1991 and 2000 dropped to 13.3 species. Since 1900, the annual number of first records associated with intentional introductions has more than doubled the number linked to unintentional introductions. Additionally, the proportion of invasive species brought through intentional introductions was larger than that brought through unintentional introductions. We highlight that while Japan experienced a rapid accumulation of alien plant species, including invasive species, by the 1950s, the rate of accumulation showed signs of saturation by 1960 and has since been slowly declining. Further deceleration and prevention of the introduction of invasive alien species, as targeted in the Kunming-Montreal Global Biodiversity Framework, may be achieved through increased investment in pathway management, especially management of intentional pathways.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135337515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-11DOI: 10.3897/neobiota.83.99508
A. Mannino, P. Balistreri, F. Mancuso, Fabio Bozzeda, Maurizio Pinna
The tropical seagrass Halophila stipulacea (Forsskål) Ascherson, 1867 entered in the Mediterranean Sea through the Suez Canal more than 100 years ago. In coastal-marine ecosystems the spatial niche of H. stipulacea is often overlapped with that of native Mediterranean Sea seagrasses and therefore it might out-compete them. Aiming to better understand its invasiveness potential, we monitored a Southern Mediterranean shallow coastal-marine water habitat from August 2010 to August 2011, where H. stipulacea co-occurred with the native seagrass Cymodocea nodosa (Ucria) Ascherson, 1870. Besides, the year-round dynamics of H. stipulacea was also monitored in four periods. To test the hypothesis that the presence/absence of H. stipulacea may have an effect on C. nodosa density, we analyzed the shoot density of C. nodosa in 8 sites, 4 sites where H. stipulacea was present (impacted sites) and 4 where H. stipulacea was absent (control sites). The results showed significant differences in C. nodosa shoot density according to the presence/absence of H. stipulacea, with the lowest values observed in sites where it co-occurred with H. stipulacea. We hypothesize that the dense rhizome-sediment net created by H. stipulacea can interfere with C. nodosa density, pushing down its rhizomes in the anoxic layer. The leaf features of H. stipulacea were generally comparable to those of other Mediterranean populations. In January 2011 a significant decline of H. stipulacea was observed, maybe related to changes in the environmental conditions that have become unfavorable (e.g. hydrodynamics, turbidity) and, unexpectedly, the seagrass disappeared in April 2011. In January, we also observed the occurrence of the green alien alga Caulerpa cylindracea Sonder, 1945 which rapidly invaded the bare substrate left by H. stipulacea.
{"title":"Searching for the competitive ability of the alien seagrass Halophila stipulacea with the autochthonous species Cymodocea nodosa","authors":"A. Mannino, P. Balistreri, F. Mancuso, Fabio Bozzeda, Maurizio Pinna","doi":"10.3897/neobiota.83.99508","DOIUrl":"https://doi.org/10.3897/neobiota.83.99508","url":null,"abstract":"The tropical seagrass Halophila stipulacea (Forsskål) Ascherson, 1867 entered in the Mediterranean Sea through the Suez Canal more than 100 years ago. In coastal-marine ecosystems the spatial niche of H. stipulacea is often overlapped with that of native Mediterranean Sea seagrasses and therefore it might out-compete them. Aiming to better understand its invasiveness potential, we monitored a Southern Mediterranean shallow coastal-marine water habitat from August 2010 to August 2011, where H. stipulacea co-occurred with the native seagrass Cymodocea nodosa (Ucria) Ascherson, 1870. Besides, the year-round dynamics of H. stipulacea was also monitored in four periods. To test the hypothesis that the presence/absence of H. stipulacea may have an effect on C. nodosa density, we analyzed the shoot density of C. nodosa in 8 sites, 4 sites where H. stipulacea was present (impacted sites) and 4 where H. stipulacea was absent (control sites). The results showed significant differences in C. nodosa shoot density according to the presence/absence of H. stipulacea, with the lowest values observed in sites where it co-occurred with H. stipulacea. We hypothesize that the dense rhizome-sediment net created by H. stipulacea can interfere with C. nodosa density, pushing down its rhizomes in the anoxic layer. The leaf features of H. stipulacea were generally comparable to those of other Mediterranean populations. In January 2011 a significant decline of H. stipulacea was observed, maybe related to changes in the environmental conditions that have become unfavorable (e.g. hydrodynamics, turbidity) and, unexpectedly, the seagrass disappeared in April 2011. In January, we also observed the occurrence of the green alien alga Caulerpa cylindracea Sonder, 1945 which rapidly invaded the bare substrate left by H. stipulacea.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44376668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-02DOI: 10.3897/neobiota.83.102975
Essmat Mohammed, Rahma Amen, Hoda M. Abdelwahab, C. Winkelmann
Spinycheek crayfish (Faxonius limosus) and signal crayfish (Pacifastacus leniusculus) are successful North American invasive crayfish species distributed throughout Europe. Both species compete with native benthic fish for shelter. In a laboratory approach, we assessed competition for shelter and antagonistic interactions between these invasive crayfish species and the native benthic fish species, stone loach (Barbatula barbatula) and bullhead (Cottus gobio). This allows for studying the potential impacts of invasive crayfish on native benthic fish. Spinycheek crayfish and signal crayfish were able to gain control of the shelter and could successfully displace both benthic fish species. For stone loach, the presence of crayfish significantly decreased their shelter use and caused several behavioural changes such as reduced activity and increased hiding behaviour outside the shelter. Although the shelter use by bullheads was not reduced, they displayed similar behavioural changes, if less intense. Invasive crayfish species showed remarkable combative interactions against both species of benthic fishes, evidenced by the high number of aggressive interactions, especially concerning stone loach. Our results highlight the pronounced dominance of invasive crayfish over benthic fish in terms of shelter competition and aggressive interactions under laboratory conditions, which consequently might promote the latter’s exposure to predation.
{"title":"Potential impacts of invasive crayfish on native benthic fish: shelter use and agonistic behaviour","authors":"Essmat Mohammed, Rahma Amen, Hoda M. Abdelwahab, C. Winkelmann","doi":"10.3897/neobiota.83.102975","DOIUrl":"https://doi.org/10.3897/neobiota.83.102975","url":null,"abstract":"Spinycheek crayfish (Faxonius limosus) and signal crayfish (Pacifastacus leniusculus) are successful North American invasive crayfish species distributed throughout Europe. Both species compete with native benthic fish for shelter. In a laboratory approach, we assessed competition for shelter and antagonistic interactions between these invasive crayfish species and the native benthic fish species, stone loach (Barbatula barbatula) and bullhead (Cottus gobio). This allows for studying the potential impacts of invasive crayfish on native benthic fish. Spinycheek crayfish and signal crayfish were able to gain control of the shelter and could successfully displace both benthic fish species. For stone loach, the presence of crayfish significantly decreased their shelter use and caused several behavioural changes such as reduced activity and increased hiding behaviour outside the shelter. Although the shelter use by bullheads was not reduced, they displayed similar behavioural changes, if less intense. Invasive crayfish species showed remarkable combative interactions against both species of benthic fishes, evidenced by the high number of aggressive interactions, especially concerning stone loach. Our results highlight the pronounced dominance of invasive crayfish over benthic fish in terms of shelter competition and aggressive interactions under laboratory conditions, which consequently might promote the latter’s exposure to predation.","PeriodicalId":54290,"journal":{"name":"Neobiota","volume":" ","pages":""},"PeriodicalIF":5.1,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45622132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：