Journal of Hospital Infection最新文献

英文中文

Antimicrobial efficacy of an experimental UV-C robot in controlled conditions and in a real hospital scenario 在受控条件下和真实医院场景中，实验性紫外线-C 机器人的抗菌功效。

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection

Pub Date : 2025-02-01 DOI: 10.1016/j.jhin.2024.11.010

B. Casini , M. Scarpaci , F. Chiovelli , S. Leonetti , A.L. Costa , M. Baroni , M. Petrillo , F. Cavallo

Background

Among no-touch automated disinfection devices, ultraviolet-C (UV-C) radiation has been proven to be one of the most effective against a broad spectrum of micro-organisms causing healthcare-associated infections.

Aim

To evaluate the antimicrobial efficacy of an experimental UV-C robotic platform, under controlled conditions and in a real hospital scenario, when used to implement the standard cleaning operating protocol (SOP).

Methods

In vitro, following dose calibration tests, bactericidal and virucidal efficacy were tested in accordance with American Society for Testing and Materials International Standard E3135-18. In hospital settings, 12 high-touch surfaces were sampled after healthcare activity (dirty condition), after SOP alone, and after SOP + UV-C treatment, with a total of 180 samples.

Findings

In vitro, <4 mJ/cm² was required to remove Staphylococcus aureus and Pseudomonas aeruginosa completely, 194 mJ/cm² was required to inactivate adenovirus HadV5 completely, and 38.8 mJ/cm² was sufficient to inactivate coronavirus 229E completely. In the real hospital scenario, the mean UV-C dose emitted on the sampled surfaces was 29.31 mJ/cm². A significant difference was found after SOP alone (P=0.022) and after SOP + UV-C treatment (P=0.007) compared with the dirty condition. The average percentage reduction in the total viable count (TVC) was 67% after SOP alone and 96% after SOP + UV-C treatment.

Conclusions

Comparison of the tests conducted in vitro and in the real hospital scenario showed that the efficacy of the UV-C robot was reduced in the hospital setting, as a higher dose was needed to obtain a reduction in the TVC.

背景：目的：评估实验性紫外线-C 机器人平台在受控条件下和实际医院场景中执行标准清洁操作规程（SOP）时的抗菌效果：方法：根据 ASTM E3135-18 标准，在体外进行剂量校准测试后，测试杀菌和杀病毒效果。在医院环境中，对医疗活动后、SOP 或 SOP 和紫外线-C 处理后的 12 个高接触表面进行了取样，共计 180 个样本：在体外，彻底清除金黄色葡萄球菌和绿脓杆菌所需的剂量小于 4 mJ/cm2，彻底灭活腺病毒 HadV5 所需的剂量为 194 mJ/cm2，而彻底灭活冠状病毒 229E 所需的剂量为 38.8 mJ/cm2。在真实场景中，采样表面发射的紫外线-C 剂量平均为 29.31 mJ/cm2。我们发现，经过 SOP 处理（p 值 = 0.022）和紫外线-C 处理（p 值 = 0.007）后获得的数据与脏污条件下获得的数据相比有明显差异。经过 SOP 处理后，总存活计数（TVC）平均减少了 67%，而经过 SOP 和紫外线-C 联合处理后，总存活计数平均减少了 96%：通过比较体外测试和实际场景中的测试，我们发现在医院环境中，紫外线-C 机器人的功效有所降低，因为与体外测试不同的是，必须使用比体外测试更高的剂量，才能使总存活数（TVC）减少，但总存活数甚至没有减少。

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引用次数: 0

Candida auris in Dutch hospitals: are we ready for it? 荷兰医院中的耳念珠菌：我们准备好了吗？

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection

Pub Date : 2025-02-01 DOI: 10.1016/j.jhin.2024.12.004

L.M.L. Dix , D.W. Notermans , C. Schneeberger , K. van Dijk

Background

Candida auris can cause nosocomial outbreaks and provides challenges concerning diagnosis, treatment, eradication and infection prevention. There are no Dutch standards or guidelines for C. auris, and current hospital practices are unknown. Therefore, we assessed whether Dutch hospitals are prepared for C. auris introduction.

Methods

An online questionnaire concerning screening, diagnostics, infection prevention and outbreaks was distributed amongst medical microbiologists and infection prevention practitioners in spring 2024.

Findings

Fifty-two questionnaires were processed comprising 58 hospitals. Most participants (60%) did not screen for C. auris carriership and 51% did not have a protocol describing screening procedures. Healthcare workers were rarely screened. Screening sites and number of swabs varied. All respondents would place a patient with C. auris in isolation, 71% had a protocol describing isolation measures. Most hospitals took extra cleaning precautions after finding C. auris. None of the hospitals ever had a C. auris-outbreak, 29% had an outbreak protocol. Procedures to cease isolation were present in 31%, but 10% never declare a patient C. auris-free. A diagnostic protocol (available in 53%) was primarily based on culture, but the execution differed. Molecular diagnostics were rarely used (12%). The majority did not screen nor did they have a protocol describing multi-drug-resistant candida outbreak coordination.

Conclusions

Screening, diagnostics, infection prevention, control and outbreak management of C. auris vary amongst Dutch hospitals, and most are not fully prepared for C. auris. As inadequate preparation for C. auris is an international concern, guidance documents could aid in fulfilling this need.

背景：耳念珠菌可引起院内暴发，对诊断、治疗、根除和预防感染提出了挑战。荷兰没有针对金黄色葡萄球菌的标准或指导方针，目前医院的做法也不得而知。因此，我们评估了荷兰医院是否为引入C. auris做好了准备。方法：于2024年春季向医学微生物学家和感染预防从业人员发放关于筛查、诊断、感染预防和暴发的在线问卷。结果：共处理问卷52份，涉及58家医院。大多数参与者（60%）没有筛查金黄色葡萄球菌携带者，51%的参与者没有描述筛查程序的方案。卫生保健工作者很少接受筛查。筛查地点和拭子数量各不相同。所有应答者都会将患有金黄色葡萄球菌的患者隔离，71%的应答者有描述隔离措施的方案。大多数医院在发现金黄色葡萄球菌后都采取了额外的清洁措施。没有一家医院爆发过金黄色葡萄球菌，29%的医院有爆发协议。31%的国家有停止隔离的程序，但10%的国家从未宣布患者无耳c菌。诊断方案（53%）主要基于培养，但执行方法不同。很少使用分子诊断（12%）。大多数没有筛查，也没有描述耐多药念珠菌爆发协调的方案。结论：荷兰各医院对金黄色葡萄球菌的筛查、诊断、感染预防、控制和疫情管理各不相同，大多数医院没有为金黄色葡萄球菌做好充分准备。由于对金黄色葡萄球菌的准备不足是一个国际问题，指导文件可以帮助满足这一需求。

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引用次数: 0

Clinical and microbiological effectiveness of pulsed-xenon ultraviolet light disinfection in a neonatal intensive care unit in Japan 脉冲氙气紫外线消毒在日本新生儿重症监护病房的临床和微生物效果。

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection

Pub Date : 2025-02-01 DOI: 10.1016/j.jhin.2024.11.015

S. Komamizu , Y. Yamamoto , K. Morikane , Y. Kuwabara , M. Kondo , K. Tatebayashi , T. Koyama , D. Terazawa

Background

Meticillin-resistant Staphylococcus aureus (MRSA) is a common causative agent of serious healthcare-related infections in neonatal intensive care units (NICUs). In adult ICUs, pulsed-xenon ultraviolet (PX-UV) disinfection of environmental surfaces, along with alcohol-based hand hygiene and terminal cleaning, has been demonstrated to reduce the MRSA acquisition rate.

Aim

To explore the impact of PX-UV use in NICUs on reducing MRSA transmission.

Methods

The incidence of newly detected MRSA cases was recorded for all patients between January 2021 and December 2022. UV irradiation was used in addition to terminal manual cleaning. During the baseline period, irradiation was performed on areas following the use by patients with MRSA; in the intervention period, irradiation was performed after each patient's use.

Findings

Though there was no observed change in the frequency of hand hygiene compliance throughout the study period, the total number of MRSA cases detected per 1000 patient-days decreased significantly.

Conclusion

In NICUs, UV irradiation of environmental surfaces, in addition to hand hygiene practices and conventional environmental maintenance, may be effective in reducing MRSA infection.

背景：耐甲氧西林金黄色葡萄球菌（MRSA）是新生儿重症监护病房（NICUs）严重卫生保健相关感染的常见病原体。在成人icu中，环境表面的脉冲氙气紫外线（PX-UV）消毒，以及基于酒精的手部卫生和终端清洁，已被证明可以降低MRSA的获得率。然而，在nicu中使用PX-UV对减少MRSA传播的影响仍未被探索。方法：记录所有患者在2021年1月至2022年12月期间新检出MRSA病例的发生率。除末端人工清洗外，还采用紫外线照射。在基线期，对MRSA患者使用后的区域进行照射，而在干预期，在每位患者使用后进行照射。结果：在整个研究期间，虽然没有观察到手部卫生依从性的频率发生变化，但每1000个患者日检测到的MRSA病例总数显着下降。结论：在新生儿重症监护病房中，环境表面的紫外线照射，加上手部卫生习惯和常规的环境维护，可能有效减少MRSA感染。

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引用次数: 0

Impact of postpartum hospital length of stay on infant gut microbiota: a comprehensive analysis of vaginal and caesarean birth 产后住院时间对婴儿肠道微生物群的影响：阴道分娩和剖腹产的综合分析。

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection

Pub Date : 2025-02-01 DOI: 10.1016/j.jhin.2024.10.012

S. Bashar , H.M. Tun , J.Y. Ting , M. Hicks , P.J. Mandhane , T.J. Moraes , E. Simons , S.E. Turvey , P. Subbarao , J.A. Scott , A.L. Kozyrskyj

Background

The primary concern with prolonged hospitalization following birth is the risk of acquiring hospital-acquired infections (HAIs) caused by opportunistic bacteria, which can alter the early establishment of gut microbiota.

Objective

To assess the association between postpartum hospital length of stay (LOS) and the composition of gut microbiota at 3 and 12 months of age according to birth mode.

Methods

In total, 1313 Canadian infants from the CHILD Cohort Study were involved in this study. Prolonged LOS was defined as ≥2 days following vaginal delivery (VD) and ≥3 days following caesarean section (CS). The gut microbiota of infants was characterized by Illumina 16S rRNA sequencing of faecal samples at 3–4 months and 12 months of age.

Findings

Following prolonged LOS, VD infants with no exposure to intrapartum antibiotics had a higher abundance of bacteria known to cause HAIs in their gut, including Enterococcus spp. at 3 and 12 months, Citrobacter spp. at 3 months, and Clostridioides difficile at 12 months. Abundance of Enterococcus spp. or Citrobacter spp. at 3 months significantly mediated the association between LOS and low abundance of Bacteroidaceae, or higher Enterococcaeae/Bacteriodaceae or Enterobacterales/Bacteroidaceae abundance ratios at 12 months of age in VD infants without intrapartum antibiotic exposure. HAI-causing Enterobacterales were also more abundant in later infancy in infants with prolonged LOS following CS. In the absence of exclusive breastfeeding at 3 months or any breastfeeding at 12 months, Porphyromonadaceae (of Bacteroidota) were depleted in CS infants with prolonged LOS.

Conclusions

Prolonged hospital stay after birth is associated with infant gut dysbiosis.

背景：产后长期住院的主要问题是有可能感染由机会性细菌引起的医院获得性感染（HAIs），这可能会改变肠道微生物群的早期建立：本研究旨在根据出生方式评估产后住院时间（LOS）与3个月和12个月时肠道微生物群组成之间的关系：本研究共涉及 1313 名加拿大婴儿，他们均来自 CHILD 队列研究（CHILD Cohort Study）。延长住院时间的定义是阴道分娩后超过 2 天和剖腹产后超过 3 天。通过对婴儿 3-4 个月和 12 个月时的粪便样本进行 Illumina 16S rRNA 测序，确定了婴儿肠道微生物群的特征：结果：在住院时间较长的阴道分娩（VD）婴儿中，母体产前未接触抗生素（IAP）的婴儿肠道中已知可导致HAIs的细菌数量较多，包括3个月和12个月时的肠球菌、3个月时的枸橼酸杆菌和12个月时的艰难梭菌。在未接触过 IAP 的 VD 婴儿中，3 个月时的肠球菌或枸橼酸杆菌丰度明显介导了 LOS 与类杆菌科低度丰度或 12 个月时较高的肠球菌科/杆菌科或肠杆菌科/类杆菌科丰度比之间的关系。在 CS 后住院时间较长的婴儿中，致人感染性疾病的肠杆菌科细菌也较多。如果没有进行 3 个月的纯母乳喂养或在 12 个月时进行任何母乳喂养，那么在住院时间较长的 CS 婴儿中，卟啉单胞菌属（类杆菌科）的数量就会减少：结论：出生后住院时间延长与婴儿肠道菌群失调有关。

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引用次数: 0

Serratia marcescens outbreak at a neonatal intensive care unit in an acute care tertiary hospital in Singapore 新加坡一家急诊三级医院的新生儿重症监护室爆发了肉豆蔻沙雷氏菌疫情。

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection

Pub Date : 2025-02-01 DOI: 10.1016/j.jhin.2024.10.002

B. Shaik Ismail , H.X. Toh , J.H. Seah , K.Y. Tan , L.C. Lee , Y.Y. Tay , K.C. Khong , A.W.M. Seet , K.C. Tesalona , A.J.H. Ngeow , S.K.Y. Ho , W.B. Poon , D.C.M. Lai , K.K.K. Ko , M.L. Ling

Background

Serratia marcescens is an aerobic Gram-negative Enterobacterales bacillus that has emerged as a cause of hospital-associated infections.

Aim

To report the epidemiological, diagnostic, and genetic investigation of an outbreak involving five neonatal patients infected or colonized with S. marcescens including the infection control interventions.

Methods

The outbreak occurred in a 28-bedded neonatal unit in an acute care tertiary hospital in Singapore divided into three areas: two negative-pressure airborne infection isolation rooms with a shared anteroom, 10 neonatal intensive care unit (NICU) beds, and 16 high-dependency beds. In-flight patients and their immediate environment were screened for S. marcescens to determine probable environmental sources. Whole-genome sequencing (WGS) analysis of resulting isolates was performed to determine clone relatedness and possible transmission patterns. Implementation of infection control interventions included prompt isolation of cases, enhanced equipment and environmental disinfection, use of alcohol-based hand rub as the preferred hand hygiene mode, enhanced infection prevention orientation for parents, review of practices, audits, and immediate feedback on non-compliance.

Findings

Five neonates infected or colonized with S. marcescens were involved in this outbreak. Four were infection cases and one was identified through contact tracing. Three NICU sinks and the milk preparation room sink were tested positive for S. marcescens. WGS confirmed clonality of strains from two NICU sinks, and milk preparation room sink with that of the five neonates.

Conclusion

A multi-prong strategy was required to contain this outbreak. WGS analysis showed association of biofilms in sinks with the outbreak.

目的报告新生儿患者感染或定植马氏沙雷氏菌（S. marcescens）疫情的流行病学、诊断和遗传学调查，包括感染控制干预措施：疫情调查报告。地点：新加坡一家急症护理三级医院的新生儿科，共设有 28 张病床，分为三个区域：两个负压空气传播感染隔离室和一个共用前厅、10 张新生儿重症监护室病床和 16 张高度依赖病床：此次疫情共涉及五名新生儿：方法：对飞行中的患者及其周围环境进行 S. marcescens 筛查，以确定可能的环境来源，对所分离的菌株进行全基因组测序 (WGS) 分析，以确定克隆相关性和可能的传播模式。感染控制干预措施的实施包括及时隔离病例、加强设备和环境消毒、使用酒精擦手液作为首选的手部卫生方式、加强对家长的感染预防指导、审查做法、审计以及对违规行为的即时反馈：结果：此次疫情共涉及五名感染或定植了马氏菌的新生儿。其中四例为感染病例，一例是通过接触追踪发现的。新生儿重症监护室的三个水槽和配奶室的水槽经检测均对马氏菌呈阳性反应。WGS 证实，新生儿重症监护室的两个水槽和配奶室的水槽中的菌株与五名新生儿的菌株具有克隆性：结论：需要采取多管齐下的策略来控制疫情。WGS 分析表明水槽中的生物膜与疫情有关。

{"title":"Serratia marcescens outbreak at a neonatal intensive care unit in an acute care tertiary hospital in Singapore","authors":"B. Shaik Ismail , H.X. Toh , J.H. Seah , K.Y. Tan , L.C. Lee , Y.Y. Tay , K.C. Khong , A.W.M. Seet , K.C. Tesalona , A.J.H. Ngeow , S.K.Y. Ho , W.B. Poon , D.C.M. Lai , K.K.K. Ko , M.L. Ling","doi":"10.1016/j.jhin.2024.10.002","DOIUrl":"10.1016/j.jhin.2024.10.002","url":null,"abstract":"<div><h3>Background</h3><div><em>Serratia marcescens</em> is an aerobic Gram-negative Enterobacterales bacillus that has emerged as a cause of hospital-associated infections.</div></div><div><h3>Aim</h3><div>To report the epidemiological, diagnostic, and genetic investigation of an outbreak involving five neonatal patients infected or colonized with <em>S. marcescens</em> including the infection control interventions.</div></div><div><h3>Methods</h3><div>The outbreak occurred in a 28-bedded neonatal unit in an acute care tertiary hospital in Singapore divided into three areas: two negative-pressure airborne infection isolation rooms with a shared anteroom, 10 neonatal intensive care unit (NICU) beds, and 16 high-dependency beds. In-flight patients and their immediate environment were screened for <em>S. marcescens</em> to determine probable environmental sources. Whole-genome sequencing (WGS) analysis of resulting isolates was performed to determine clone relatedness and possible transmission patterns. Implementation of infection control interventions included prompt isolation of cases, enhanced equipment and environmental disinfection, use of alcohol-based hand rub as the preferred hand hygiene mode, enhanced infection prevention orientation for parents, review of practices, audits, and immediate feedback on non-compliance.</div></div><div><h3>Findings</h3><div>Five neonates infected or colonized with <em>S. marcescens</em> were involved in this outbreak. Four were infection cases and one was identified through contact tracing. Three NICU sinks and the milk preparation room sink were tested positive for <em>S. marcescens</em>. WGS confirmed clonality of strains from two NICU sinks, and milk preparation room sink with that of the five neonates.</div></div><div><h3>Conclusion</h3><div>A multi-prong strategy was required to contain this outbreak. WGS analysis showed association of biofilms in sinks with the outbreak.</div></div>","PeriodicalId":54806,"journal":{"name":"Journal of Hospital Infection","volume":"156 ","pages":"Pages 21-25"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142513203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaccination of healthcare workers against mpox: a call for action 医护人员接种麻风腮疫苗：行动呼吁。

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection

Pub Date : 2025-02-01 DOI: 10.1016/j.jhin.2024.10.007

G. Lippi , B.M. Henry , J.G. Rizk

引用次数: 0

Multi-locus sequence typing of Candida tropicalis among Candiduria shows an outbreak in azole-susceptible isolates and clonal cluster enriched in azole-resistant isolates 念珠菌间热带念珠菌多位点序列分型显示在唑敏感分离株中爆发，在唑耐药分离株中富集克隆簇。

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection

Pub Date : 2025-02-01 DOI: 10.1016/j.jhin.2024.11.018

L. Zhao , L.F. Xu , G.D. Xiang , Q.C. Zhou , Y. Wang , G.Y. Li

Background

The increasing detection rate of C. tropicalis and its azole resistance have made clinical treatment difficult. The presence of candiduria seems to correlate with invasive candida infection, especially for patients admitted to ICUs. However, the prevalence and antifungal resistance of C. tropicalis isolates in urine samples has not been well studied.

Aim

To retrospectively investigate the clinical features, antifungal resistance, and genetic relatedness of C. tropicalis isolates from urine samples.

Methods

A total of 107 clinical C. tropicalis isolates were retrospectively studied, including phenotypes of isolates and characteristics of patients. The genetic profiles of 107 isolates were genotyped using multi-locus sequence typing (MLST). Phylogenetic analysis was inferred using unweighted pair group method with arithmetic averages. MLST clonal clusters (CCs) were analysed by goeBURST.

Findings

Of the 107 isolates, 27.1% were resistant to fluconazole, and there was a notable increasing trend of fluconazole resistance from 16.1% in 2019 to 40.0% in 2021. Forty-seven diploid sequence types (DSTs) were assigned to ten major CCs. CC1 was the predominant fluconazole-susceptible group; 24 isolates from CC1 belonged to DST333, an outbreak clone in NICU ward. The azole-resistant CC4 contained 19 isolates, accounting for 65.5% of the azole-resistant isolates in this study. CC4 belongs to a prevalent FNS CC1 globally, of which the putative founder genotype was DST225.

Conclusion

This study revealed an outbreak of azole-susceptible C. tropicalis isolates in urine specimens and a high azole resistance rate of C. tropicalis in candiduria, and the MLST type showed clonal aggregation in azole-resistant isolates from urine samples.

背景：热带念珠菌的检出率不断上升，且对唑类药物产生抗药性，给临床治疗带来了困难。念珠菌尿的出现似乎与侵袭性念珠菌感染有关，尤其是对重症监护病房的患者而言。然而，关于尿液样本中分离出的热带念珠菌的流行率和抗真菌耐药性的研究并不多：回顾性研究尿液样本中分离出的热带真菌的临床特征、抗真菌耐药性和遗传相关性。采用多焦点序列分型法（MLST）对107株分离株进行基因分型。采用算术平均非加权成对分组法（UPGMA）进行了系统发育分析。用 goeBURST 分析了 MLST 克隆群（CC）：在107个分离株中，27.1%对氟康唑具有耐药性，氟康唑耐药性呈明显上升趋势，从2019年的16.1%上升到2021年的40.0%。47个二倍体序列类型（DST）被分配给10个主要的CC。CC1是主要的氟康唑耐药群体，CC1中的24个分离株属于DST333，这是NICU病房中的一个爆发性克隆。耐唑的 CC4 包含 19 个分离株，占本研究中耐唑分离株的 65.5%。CC4属于全球流行的FNS CC1，其推测的创始基因型为DST225：我们首次揭示了尿液标本中对唑类敏感的热带念珠菌分离株的爆发和念珠菌尿症中热带念珠菌的高唑耐药率，并且 MLST 类型显示尿液标本中唑耐药分离株的克隆聚集。

{"title":"Multi-locus sequence typing of Candida tropicalis among Candiduria shows an outbreak in azole-susceptible isolates and clonal cluster enriched in azole-resistant isolates","authors":"L. Zhao , L.F. Xu , G.D. Xiang , Q.C. Zhou , Y. Wang , G.Y. Li","doi":"10.1016/j.jhin.2024.11.018","DOIUrl":"10.1016/j.jhin.2024.11.018","url":null,"abstract":"<div><h3>Background</h3><div>The increasing detection rate of <em>C. tropicalis</em> and its azole resistance have made clinical treatment difficult. The presence of candiduria seems to correlate with invasive candida infection, especially for patients admitted to ICUs. However, the prevalence and antifungal resistance of <em>C. tropicalis</em> isolates in urine samples has not been well studied.</div></div><div><h3>Aim</h3><div>To retrospectively investigate the clinical features, antifungal resistance, and genetic relatedness of <em>C. tropicalis</em> isolates from urine samples.</div></div><div><h3>Methods</h3><div>A total of 107 clinical <em>C. tropicalis</em> isolates were retrospectively studied, including phenotypes of isolates and characteristics of patients. The genetic profiles of 107 isolates were genotyped using multi-locus sequence typing (MLST). Phylogenetic analysis was inferred using unweighted pair group method with arithmetic averages. MLST clonal clusters (CCs) were analysed by goeBURST.</div></div><div><h3>Findings</h3><div>Of the 107 isolates, 27.1% were resistant to fluconazole, and there was a notable increasing trend of fluconazole resistance from 16.1% in 2019 to 40.0% in 2021. Forty-seven diploid sequence types (DSTs) were assigned to ten major CCs. CC1 was the predominant fluconazole-susceptible group; 24 isolates from CC1 belonged to DST333, an outbreak clone in NICU ward. The azole-resistant CC4 contained 19 isolates, accounting for 65.5% of the azole-resistant isolates in this study. CC4 belongs to a prevalent FNS CC1 globally, of which the putative founder genotype was DST225.</div></div><div><h3>Conclusion</h3><div>This study revealed an outbreak of azole-susceptible <em>C. tropicalis</em> isolates in urine specimens and a high azole resistance rate of <em>C</em>. <em>tropicalis</em> in candiduria, and the MLST type showed clonal aggregation in azole-resistant isolates from urine samples.</div></div>","PeriodicalId":54806,"journal":{"name":"Journal of Hospital Infection","volume":"156 ","pages":"Pages 96-105"},"PeriodicalIF":3.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of a polyhexanide-based antiseptic skin solution on Candida auris colonization and invasive fungaemia 聚己内酯皮肤消毒液对白色念珠菌定植和侵袭性真菌病的影响

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection

Pub Date : 2025-02-01 DOI: 10.1016/j.jhin.2024.11.004

K. Charisi , I. Galanis , C. Zarras , G. Totikidis , D. Kouroupis , E. Massa , C. Michailidou , S. Goumperi , E. Kosmidou , C. Alektoridou , D. Vlachakis , E. Mouloudi , P. Pateinakis , A. Pyrpasopoulou , C. Antachopoulos

引用次数: 0

Screening for latent tuberculosis infection among healthcare workers at recruitment in a country with a low incidence of tuberculosis: a retrospective study 在一个结核病发病率较低的国家对招聘的医护人员进行潜伏结核感染筛查：一项回顾性研究。

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection

Pub Date : 2025-02-01 DOI: 10.1016/j.jhin.2024.11.007

C. Vieira Gomes , S. Rusca , A. Dumoulin , P-O. Bridevaux , L. Arlettaz , S. Emonet , G. Catho

引用次数: 0

What doesn’t kill biofilm, makes them stronger: critical methodological considerations for endoscope reprocessing 什么不会杀死生物膜，使它们更强：内窥镜再处理的关键方法学考虑。

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection

Pub Date : 2025-02-01 DOI: 10.1016/j.jhin.2024.06.024

R. Kanaujia , A. Sharma , M. Biswal , V. Singh

引用次数: 0

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