Geochemical variations in thermal spring compositions are sensitive to changes in regional stress, temperature, and space conditions, which are indicators of seismic and tectonic activities. However, the temporal and spatial variations in water chemistry components and their inducing mechanisms are insufficiently understood. This study aims to evaluate the effective temporal coincidence/lag of seismic events based on the long-term continuous water chemistry monitoring of seismic areas and draw attention to water chemistry changes in thermal waters during earthquake hazard monitoring. We investigated the major elements, trace elements, and hydrogen and oxygen isotopes of 21 thermal springs along the Lancang–Gengma fault (LGF) zone, which is in a highly deformed, seismically active area of the Southeastern margin of the Tibetan Plateau. In the LGF zone, the temperatures of the studied thermal springs range from 44.7°C to 96.2°C. The reservoir temperatures range from 91°C to 195°C and the reservoir depth range from 4–9 km. According to the spatiotemporal chemical variations from two-year regular monitoring of Na+, Cl−, F−, SO42−, Li, B, δD, and δ18O in five thermal springs, the thermal springs in these fault-controlled areas are sensitive and responsive to seismic activity. Different tectonic regions exhibit significant and diverse short-term precursory anomalies in water chemistry before earthquakes with M ≥ 5.0. Their characteristics and mechanisms are region-specific, and the study area is divided into two monitoring capacity range areas. Area 1: The MG, MM and XF monitoring points located on the Baoshan block are highly sensitive to seismic responses in the SW direction of the study area. For example, continuous Na⁺, Cl−, SO42−and F− anomalies were observed in MG, MM and XF before the ML5.0 and ML5.9 Myanmar earthquakes (southwest of the study area). Area 2: The NKL and EL monitoring points located on the Simao block are highly sensitive to seismic responses in the SE direction. For instance, the Na⁺ concentrations in EL and NKL sharply increased above the normal threshold before the ML6.2 Laos earthquake and ML5.0 Honghe earthquake. Notably, significant spatial directional differences in fluid chemical responses to earthquakes were observed. This directional difference may be related to the complexity of the regional stress field in the area and the local characteristics of fault activities. The chemical composition changes of these thermal springs during fluid circulation in the LGF can be a good tracer of seismic activity.
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