Herein, we provide an overview of intra- and interspecies variability of infrared spectra of wood in the fingerprint region (1800 –800 cm− 1). The study is based on > 5000 FTIR spectra of 108 trees comprising 20 tree and shrub species that are common and characterize the landscape in Estonia. A larger share of spectral variability was attributed to differences between annual growth rings, with a smaller contribution from differences between individual trees and growing sites. Although all species have their characteristic spectral features that differentiate them from other species, intraspecies spectral variability strongly exceeds interspecies variability. It was found that, on average, infrared spectra is not affected by sampling direction on the growing site. 25 distinct infrared bands were detected based on first and second derivative spectra. It was possible to distinguish 16 spectral regions - every species had at least one peak in 15 of these and 100% of spectra of softwoods had a peak in the 16th region at around 1265 cm− 1. Principal component analysis revealed that spectral variability is not driven by particular spectral bands, but rather by contributions from a broad range of wavenumbers across the entire analyzed region.