ON THE INNER FRONT COVER: Actin filaments (green) terminating in focal adhesions stained for vinculin (red) in bovine aortic endothelial cells cultured on polydimethylsiloxane. Nuclei are stained using dapi (blue).
ON THE FRONT COVER: Normal human breast epithelial cells expressing GFP-LIMD1 (green) and tained for F-actin (red) and DNA (blue).
Credit: Samriddha Ray, Chamika DeSilva, Natasha Cruz-Calderon and Daniel McCollum. University of Massachusetts Chan Medical School Department of Biochemistry and Molecular Biotechnology.
{"title":"Front Cover Image","authors":"","doi":"10.1002/cm.21937","DOIUrl":"https://doi.org/10.1002/cm.21937","url":null,"abstract":"<p>ON THE FRONT COVER: Normal human breast epithelial cells expressing GFP-LIMD1 (green) and tained for F-actin (red) and DNA (blue).</p><p>Credit: Samriddha Ray, Chamika DeSilva, Natasha Cruz-Calderon and Daniel McCollum. University of Massachusetts Chan Medical School Department of Biochemistry and Molecular Biotechnology.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 9-10","pages":"C1"},"PeriodicalIF":2.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21937","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ON THE INNER BACK COVER: Image of the skeletal muscles showing the striated localization pattern of metastasis associated 1 (MTA1).
Credit: Chundong Gu (The First Affiliated Hospital of Dalian Medical University, Dalian, China) and Ming-Fei Lang (Medical College, Dalian University, Dalian, China)
{"title":"Inner Back Cover Image","authors":"","doi":"10.1002/cm.21939","DOIUrl":"https://doi.org/10.1002/cm.21939","url":null,"abstract":"<p>ON THE INNER BACK COVER: Image of the skeletal muscles showing the striated localization pattern of metastasis associated 1 (MTA1).</p><p>Credit: Chundong Gu (The First Affiliated Hospital of Dalian Medical University, Dalian, China) and Ming-Fei Lang (Medical College, Dalian University, Dalian, China)\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 9-10","pages":"C3"},"PeriodicalIF":2.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cm.21939","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Picture of the month by Chundong Gu and Ming-Fei Lang","authors":"","doi":"10.1002/cm.21931","DOIUrl":"10.1002/cm.21931","url":null,"abstract":"","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 9-10","pages":"506"},"PeriodicalIF":2.4,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Picture of the month by Samriddha Ray, Chamika DeSilva, Natasha Cruz-Calderon, and Daniel McCollum","authors":"","doi":"10.1002/cm.21930","DOIUrl":"10.1002/cm.21930","url":null,"abstract":"","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 9-10","pages":"505"},"PeriodicalIF":2.4,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Remembrance of Robert S. Adelstein: Mr. Nonmuscle Myosin 2","authors":"James R. Sellers","doi":"10.1002/cm.21948","DOIUrl":"10.1002/cm.21948","url":null,"abstract":"","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 12","pages":"718-722"},"PeriodicalIF":2.4,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Author Profile: Paul Griffin","authors":"Paul J. Griffin","doi":"10.1002/cm.21919","DOIUrl":"10.1002/cm.21919","url":null,"abstract":"","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 11","pages":"695-696"},"PeriodicalIF":2.4,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142382612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Remembrance of Edward D. Korn: A Pioneer in Our Field","authors":"John A. Hammer","doi":"10.1002/cm.21942","DOIUrl":"10.1002/cm.21942","url":null,"abstract":"","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 12","pages":"713-717"},"PeriodicalIF":2.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142333683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Picture of the month by Aparna Bhattacharyya and Kenneth Barbee","authors":"","doi":"10.1002/cm.21932","DOIUrl":"10.1002/cm.21932","url":null,"abstract":"","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"81 9-10","pages":"507"},"PeriodicalIF":2.4,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142333682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gülseren Özduman, Faruk Şimşek, Aadil Javed, Kemal Sami Korkmaz
Hematological and neurological expressed 1 (HN1) is homolog of Jupiter protein from Drosophila melanogaster where it functions as a microtubule‐associated protein. However, in mammalian cells, HN1 is associated partially with y‐tubulin in centrosomes, Stathmin for stabilizing microtubules, and Cdh1 for regulating Cyclin B1 for cell cycle regulation. Moreover, HN1 overexpression leads to early mitotic exit as well. Other molecular functions and interactions of HN1 are not clear yet. Here, based on our previous analysis where HN1 was shown to cluster supernumerary centrosomes and maintain mitotic spindle assembly, we further investigated the role of HN1 in centrosome maintenance and mitotic fidelity in PC‐3 prostate and MDA‐MB231 mammary cancer cell lines. The maturation‐associated roles of HN1 during cell division by examining the AuroraA‐PLK1 axis involving a plus end kinesin, Eg5 as well as pericentriolar matrix protein (PCM1) as components of centrosomes were established. We found that HN1 co‐localized to centrioles with Eg5 and Aurora A to suppress aberrant spindle formation to ensure the fidelity of centriole/centrosome duplication when overexpressed. Consistently, depleting the HN1 expression using siRNA or shRNA resulted in an increased number of dysregulated mitotic spindle structures, where Aurora A as well as PLK1 co‐localizations with Eg5 and PCM1 were disrupted. Further, the PLK1 and Aurora A kinase's phosphorylations also decreased, confirming the hypothesis that the cells struggle in mitotic progression, display nuclear and cytokinetic abnormalities with supernumerary but immature mononucleated centrosomes. In summary, we described the role of HN1 in centrosome nucleation/maturation in PLK1‐Eg5 axis and concomitant mitotic spindle formation in human cells.
{"title":"HN1 expression contributes to mitotic fidelity through Aurora A‐PLK1‐Eg5 axis","authors":"Gülseren Özduman, Faruk Şimşek, Aadil Javed, Kemal Sami Korkmaz","doi":"10.1002/cm.21928","DOIUrl":"https://doi.org/10.1002/cm.21928","url":null,"abstract":"Hematological and neurological expressed 1 (HN1) is homolog of Jupiter protein from <jats:italic>Drosophila melanogaster</jats:italic> where it functions as a microtubule‐associated protein. However, in mammalian cells, HN1 is associated partially with y‐tubulin in centrosomes, Stathmin for stabilizing microtubules, and Cdh1 for regulating Cyclin B1 for cell cycle regulation. Moreover, HN1 overexpression leads to early mitotic exit as well. Other molecular functions and interactions of HN1 are not clear yet. Here, based on our previous analysis where HN1 was shown to cluster supernumerary centrosomes and maintain mitotic spindle assembly, we further investigated the role of HN1 in centrosome maintenance and mitotic fidelity in PC‐3 prostate and MDA‐MB231 mammary cancer cell lines. The maturation‐associated roles of HN1 during cell division by examining the AuroraA‐PLK1 axis involving a plus end kinesin, Eg5 as well as pericentriolar matrix protein (PCM1) as components of centrosomes were established. We found that HN1 co‐localized to centrioles with Eg5 and Aurora A to suppress aberrant spindle formation to ensure the fidelity of centriole/centrosome duplication when overexpressed. Consistently, depleting the HN1 expression using siRNA or shRNA resulted in an increased number of dysregulated mitotic spindle structures, where Aurora A as well as PLK1 co‐localizations with Eg5 and PCM1 were disrupted. Further, the PLK1 and Aurora A kinase's phosphorylations also decreased, confirming the hypothesis that the cells struggle in mitotic progression, display nuclear and cytokinetic abnormalities with supernumerary but immature mononucleated centrosomes. In summary, we described the role of HN1 in centrosome nucleation/maturation in PLK1‐Eg5 axis and concomitant mitotic spindle formation in human cells.","PeriodicalId":55186,"journal":{"name":"Cytoskeleton","volume":"52 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142267444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：