Biopreservation and Biobanking最新文献

Introduction: Biobanks and sample collections are critical resources for advancing biomedical research and public health. However, in Latin America and the Caribbean (LAC), challenges related to infrastructure, governance, and regulatory frameworks hinder the development of sustainable and collaborative biobanking networks. The adoption of FAIR (Findable, Accessible, Interoperable, and Reusable) principles is increasingly recognized as a key strategy to enhance the long-term sustainability and scientific impact of these networks. Objectives: This study explored the political, technical, ethical, and regulatory challenges of establishing a sustainable regional biobank network in LAC, with particular attention to the integration of FAIR principles. Methods: A qualitative-exploratory study was conducted through semi-structured interviews with 14 representatives of biobanks and sample collections from seven LAC countries (Argentina, Brazil, Colombia, Mexico, Peru, Chile, and Puerto Rico). Thematic analysis was applied to identify key challenges and opportunities. Results: Participants' perceptions revealed significant barriers, such as insufficient infrastructure, lack of standardized biobank management systems, regulatory barriers, and limited inter-institutional collaboration. While the interview guide did not explicitly focus on the FAIR principles, many of the issues raised, such as the need for data standardization, interoperable systems, and improved data accessibility, were aligned with FAIR principles. Participants also emphasized the importance of international cooperation to expand technical expertise, ensure ethical oversight, and improve financial sustainability. Key ethical concerns included inconsistencies in informed consent practices, data privacy regulations, and community engagement in biobank-related decision-making. Conclusion: Addressing these barriers requires harmonized policies, investment in biobanking infrastructure, and long-term sustainability strategies. Strengthening capacity-building programs and integrating the FAIR principles can support the development of robust, collaborative biobank networks, enhancing equitable access to biospecimens and maximizing their value for biomedical research and public health in LAC.

The Brazilian Caatinga biome, a hotspot of unique biodiversity, faces escalating threats from habitat loss and climate change. Over the past two decades, significant progress has been made in developing reproductive biotechnologies to preserve the genetic diversity of native species through germplasm biobanking. This review synthesizes pioneering work by the Laboratory of Animal Germplasm Conservation, Federal University of the Semiarid Region, detailing standardized protocols for cryopreserving sperm, ovarian follicles, and gonadal tissues from key Caatinga species, including collared peccaries, agoutis, cavies, jaguars, and rheas. We critically evaluate the successes and limitations of the current biobanking initiatives, emphasizing species-specific challenges in gamete and tissue preservation. Furthermore, we address systemic barriers, such as fragmented public policies, logistical constraints, and the urgent need for scalable infrastructure, to expanding biobanking efforts across this understudied biome. Finally, we propose suggestions for integrating biobanks with assisted reproductive technologies and global conservation networks, highlighting their role as a genetic safeguard for endangered fauna.

The accelerating loss of biodiversity and genetic diversity poses an urgent threat to ecosystems and future generations. This concept article proposes a model centered on biobanks and advanced reproductive biotechnologies as strategic conservation tools, particularly within biodiversity hotspots such as Misiones, Argentina. We outline how institutions like biodiversity institutes, zoos, and biotech firms can collaborate to develop decentralized, scalable cryopreservation capacity. Emphasizing the IMiBio model, we explore how international collaboration, ethical frameworks, and locally grounded governance can support the preservation and future restoration of species. This approach supports both conservation and regeneration, providing a roadmap for hotspot-centered biodiversity recovery.^1-3.

Biobanks provide essential resources for research, yet little is known about their development in Latin America. This study reports the results of a cross-sectional survey conducted by the International Society for Biological and Environmental Repositories among Latin American biobanks between February and June 2021, during the second year of the COVID-19 pandemic. In total, 56 biobanks from seven countries responded to the survey, answering questions that included their institutional affiliations, funding, governance, and access policies. Most biobanks (94%) are linked to public or academic institutions, with human health research, particularly oncology, as the primary focus. Financial sustainability is a major concern, with most biobanks relying on institutional or grant-based funding. While many biobanks report adherence to international standard operating procedures and openness to share samples and associated data, challenges in data standardization and collaboration persist. Strengthening regional integration, harmonizing governance policies, and ensuring sustainable funding strategies are critical for enhancing the role of biobanks in Latin American and global research.

Global measures and restrictions implemented to control the COVID-19 pandemic had a significant impact and created challenges in regular business, commerce, and scientific activities, including biobank operations. The challenges were exacerbated by a dependence on imported supplies and reagents from countries such as the United States, China, and within Europe, as well as restrictions on exporting biological samples. The pandemic has highlighted the pressing need for regional autonomy in biobanking activities. This article aims to enhance the capacity of biobanks to respond effectively to future crises. Recognizing the importance of adaptability and resilience, our study highlights the specific strategies employed by a large biobank in Costa Rica to ensure the continuity of operations during unprecedented times. During the COVID-19 pandemic, biobanking operations encountered a number of critical challenges, which led to the identification, implementation, and integration of targeted mitigation strategies into our contingency framework. Five challenges were identified and subsequently addressed: disruptions and shortfalls in the supply of essential material, continuity of daily biobank operations, storage capacity constraints coupled with export limitations, difficulties associated with consent procedures, and complications surrounding personnel testing. These lessons underscore the critical importance of contingency planning, digitization of biobanking workflows, and increased regional collaboration to strengthen the autonomy and resilience of biobanks in the face of future crises.

Epiphytic yeasts are promising biocontrol agents of plant diseases but preserving and transferring them to the field is challenging. Here, we studied six cost-effective lyophilization protective agents to preserve seven strains of Amazonian yeast species isolated from the phyllosphere of native cacao (Theobroma cacao) in Peru. We evaluated the viability of yeasts at 30 and 90 days post-lyophilization in vitro, and their survival after controlled inoculation on cacao fruits in the field. The best protective agents were maltodextrin, honey + skim milk, and honey. Wickerhamomyces anomalus KLG-014 and Wickerhamomyces sp. EGZ-38 showed higher than 97.3% viability after 30 days when lyophilized with maltodextrin. Additionally, Candida sp. KLG-103 showed a viability greater than 50% after 30 days when lyophilized with honey + skim milk. At 90 days, W. anomalus KLG-014, Hannaella theobromatis KLG-063, and Kwoniella heveanensis EGZ-07 showed a viability greater than 20%, with the latter showing an outstanding 100% viability, when lyophilized with honey + skim milk. Conversely, sodium alginate was the least protective agent, as yeast showed 0% viability. In the field, W. anomalus KLG-014, K. heveanensis EGZ-07, Debaryomyces hansenii EGZ-31, and Wickerhamomyces sp. EGZ-38 were successfully re-isolated from the surface of cacao fruits under all treatments after 30 days, except for sodium alginate. This was corroborated via morphological and molecular evidence. This study demonstrates that maltodextrin, honey, and skim milk are suitable for ensuring the in vitro viability of biocontrol yeasts up to 90 days after lyophilization, and their survival up to 30 days after inoculation on cacao fruits in the field. This is a first step toward the development of a biocontrol alternative to mitigate cacao pathogens using native microorganisms from the Amazon in Peru.

Sampling skin fragments has been an important strategy for genetic studies and ex situ conservation, aiding in the preservation of genetic diversity in Neotropical deer and other wild species. From the moment of collection in the field, transport media must ensure tissue viability by providing the necessary nutrients until laboratory processing for culture or cryopreservation. This study aimed to evaluate the effects of temperature and storage duration on tissue viability and cell growth using two types of skin transport media: Dulbecco's modified Eagle medium (DMEM) supplemented with 15% fetal bovine serum and 0.9% physiological saline solution. Skin fragments were collected from the inguinal region of five captive gray-brocket deer (Subulo gouazoubira) and divided into small samples, which were randomly assigned to each transport medium. The samples were stored at 5°C and 24°C for 24 and 72 hours, followed by cryopreservation and thawing to assess histomorphology, apoptosis (TUNEL test), cell growth, viability (Trypan blue and MTT assay), and mitotic index. The results showed that physiological saline solution is as efficient as DMEM in maintaining tissue viability, with 80% of viable cells observed and no significant difference after storing in different skin transport media (p > 0.05). Cell morphology and apoptosis did not change in response to media, temperature, or storage duration. We recovered metaphases from all skin tissue storing conditions, with a similar mitotic index to those presented in other cell culture studies from deer biopsies. These results showed the feasibility of storing skin tissue samples during 24 and 72 hours at 5°C and 24°C in different transport media guaranteeing the cell growth and viability for genetic studies and reproductive biotechnologies. The study may contribute to sampling collection in places where displacement with large equipment is limited, allowing the establishment of simplified skin transport protocols as an important step to accessing genetic material from individuals inhabiting isolated localities.

The AIDS and Cancer Specimen Resource (ACSR) has developed a global biorepository network to support research on AIDS-defining and non-AIDS-defining cancers. This article details the establishment of a dedicated HIV-associated cancer biorepository in São Paulo, Brazil, a region with a high burden of these malignancies. The repository addresses the need for high-quality, well-annotated biospecimens from Latin American (LATAM) populations to support research on cancer pathogenesis in people with HIV (PWH), viral reservoirs, and clinical outcomes. It systematically collects and links biospecimens with demographic and clinical data, providing a resource for investigators. Developed with international ethics, community engagement, and regulatory standards, the biorepository is modeled after similar efforts in low- and middle-income countries. This article outlines its implementation, including sample acquisition, infrastructure, inventory management, data governance, and research collaboration. By expanding access to biospecimens, the ACSR supports research that can improve outcomes for PWH and cancer, while strengthening research capacity in the LATAM region.

Objectives: This study compared the synthetic polymer (SP) and the antifreeze protein type 3 (AFP3) protocols for the vitrification of bovine cumulus-oocyte complexes (COCs). Methods: Fresh bovine COCs were subjected to in vitro maturation (IVM) for 24 hours, while other COCs were vitrified using the SP or AFP protocols. After vitrification and warming, the COCs were subjected to IVM for 24 hours. Both fresh and vitrified COCs were analyzed for chromatin status, mitochondrial activity, reactive oxygen species levels, integrity of TZPs, DNA damage, and the expression of MPS1, BUB1, MAD1, CX43, and ZP3. Results: The metaphase II (MII) rates of COCs vitrified with SPp (38%) were significantly higher than those vitrified with AFP3p (10%) (p < 0.05). The fluorescence intensity for CM-H₂DCFDA (30 ± 3.2) and nitrite/nitrate levels (10.6 ± 1.6) were higher in AFP3p COCs (p < 0.05). The transzonal projections (TZPs) of SPp COCs were intact and showed less DNA damage (25 ± 1.15) compared with those of AFP3p (43 ± 3.9) COCs (p < 0.05). The expression of the MPS1 (SPp 0.3 ± 0.4; AFP3p 0.07 ± 0.06) and BUB1 (SPp: 0.2 ± 0.4; AFP3p 0.005 ± 0.005) genes was higher in vitrified COCs compared with fresh control COCs (0.001 ± 0.0006; 0.001 ± 4.0) (p < 0.05). On the other hand, the MAD1, CX43, and ZP3 genes were expressed only in fresh oocytes. Conclusion: Under the conditions tested, SPp was the most suitable protocol for vitrifying bovine COCs, guaranteeing good MII rates, maintaining TZP integrity and reducing DNA damage.