Biopreservation and Biobanking最新文献

Epiphytic yeasts are promising biocontrol agents of plant diseases but preserving and transferring them to the field is challenging. Here, we studied six cost-effective lyophilization protective agents to preserve seven strains of Amazonian yeast species isolated from the phyllosphere of native cacao (Theobroma cacao) in Peru. We evaluated the viability of yeasts at 30 and 90 days post-lyophilization in vitro, and their survival after controlled inoculation on cacao fruits in the field. The best protective agents were maltodextrin, honey + skim milk, and honey. Wickerhamomyces anomalus KLG-014 and Wickerhamomyces sp. EGZ-38 showed higher than 97.3% viability after 30 days when lyophilized with maltodextrin. Additionally, Candida sp. KLG-103 showed a viability greater than 50% after 30 days when lyophilized with honey + skim milk. At 90 days, W. anomalus KLG-014, Hannaella theobromatis KLG-063, and Kwoniella heveanensis EGZ-07 showed a viability greater than 20%, with the latter showing an outstanding 100% viability, when lyophilized with honey + skim milk. Conversely, sodium alginate was the least protective agent, as yeast showed 0% viability. In the field, W. anomalus KLG-014, K. heveanensis EGZ-07, Debaryomyces hansenii EGZ-31, and Wickerhamomyces sp. EGZ-38 were successfully re-isolated from the surface of cacao fruits under all treatments after 30 days, except for sodium alginate. This was corroborated via morphological and molecular evidence. This study demonstrates that maltodextrin, honey, and skim milk are suitable for ensuring the in vitro viability of biocontrol yeasts up to 90 days after lyophilization, and their survival up to 30 days after inoculation on cacao fruits in the field. This is a first step toward the development of a biocontrol alternative to mitigate cacao pathogens using native microorganisms from the Amazon in Peru.

Sampling skin fragments has been an important strategy for genetic studies and ex situ conservation, aiding in the preservation of genetic diversity in Neotropical deer and other wild species. From the moment of collection in the field, transport media must ensure tissue viability by providing the necessary nutrients until laboratory processing for culture or cryopreservation. This study aimed to evaluate the effects of temperature and storage duration on tissue viability and cell growth using two types of skin transport media: Dulbecco's modified Eagle medium (DMEM) supplemented with 15% fetal bovine serum and 0.9% physiological saline solution. Skin fragments were collected from the inguinal region of five captive gray-brocket deer (Subulo gouazoubira) and divided into small samples, which were randomly assigned to each transport medium. The samples were stored at 5°C and 24°C for 24 and 72 hours, followed by cryopreservation and thawing to assess histomorphology, apoptosis (TUNEL test), cell growth, viability (Trypan blue and MTT assay), and mitotic index. The results showed that physiological saline solution is as efficient as DMEM in maintaining tissue viability, with 80% of viable cells observed and no significant difference after storing in different skin transport media (p > 0.05). Cell morphology and apoptosis did not change in response to media, temperature, or storage duration. We recovered metaphases from all skin tissue storing conditions, with a similar mitotic index to those presented in other cell culture studies from deer biopsies. These results showed the feasibility of storing skin tissue samples during 24 and 72 hours at 5°C and 24°C in different transport media guaranteeing the cell growth and viability for genetic studies and reproductive biotechnologies. The study may contribute to sampling collection in places where displacement with large equipment is limited, allowing the establishment of simplified skin transport protocols as an important step to accessing genetic material from individuals inhabiting isolated localities.

The AIDS and Cancer Specimen Resource (ACSR) has developed a global biorepository network to support research on AIDS-defining and non-AIDS-defining cancers. This article details the establishment of a dedicated HIV-associated cancer biorepository in São Paulo, Brazil, a region with a high burden of these malignancies. The repository addresses the need for high-quality, well-annotated biospecimens from Latin American (LATAM) populations to support research on cancer pathogenesis in people with HIV (PWH), viral reservoirs, and clinical outcomes. It systematically collects and links biospecimens with demographic and clinical data, providing a resource for investigators. Developed with international ethics, community engagement, and regulatory standards, the biorepository is modeled after similar efforts in low- and middle-income countries. This article outlines its implementation, including sample acquisition, infrastructure, inventory management, data governance, and research collaboration. By expanding access to biospecimens, the ACSR supports research that can improve outcomes for PWH and cancer, while strengthening research capacity in the LATAM region.

Objectives: This study compared the synthetic polymer (SP) and the antifreeze protein type 3 (AFP3) protocols for the vitrification of bovine cumulus-oocyte complexes (COCs). Methods: Fresh bovine COCs were subjected to in vitro maturation (IVM) for 24 hours, while other COCs were vitrified using the SP or AFP protocols. After vitrification and warming, the COCs were subjected to IVM for 24 hours. Both fresh and vitrified COCs were analyzed for chromatin status, mitochondrial activity, reactive oxygen species levels, integrity of TZPs, DNA damage, and the expression of MPS1, BUB1, MAD1, CX43, and ZP3. Results: The metaphase II (MII) rates of COCs vitrified with SPp (38%) were significantly higher than those vitrified with AFP3p (10%) (p < 0.05). The fluorescence intensity for CM-H₂DCFDA (30 ± 3.2) and nitrite/nitrate levels (10.6 ± 1.6) were higher in AFP3p COCs (p < 0.05). The transzonal projections (TZPs) of SPp COCs were intact and showed less DNA damage (25 ± 1.15) compared with those of AFP3p (43 ± 3.9) COCs (p < 0.05). The expression of the MPS1 (SPp 0.3 ± 0.4; AFP3p 0.07 ± 0.06) and BUB1 (SPp: 0.2 ± 0.4; AFP3p 0.005 ± 0.005) genes was higher in vitrified COCs compared with fresh control COCs (0.001 ± 0.0006; 0.001 ± 4.0) (p < 0.05). On the other hand, the MAD1, CX43, and ZP3 genes were expressed only in fresh oocytes. Conclusion: Under the conditions tested, SPp was the most suitable protocol for vitrifying bovine COCs, guaranteeing good MII rates, maintaining TZP integrity and reducing DNA damage.

This article highlights Peru's experience in establishing a national tumor bank network, serving as a model for low- and middle-income countries. Launched in 2005 at the National Institute of Neoplastic Diseases, efforts accelerated under the 2021 National Cancer Act, which formalized the National Tumor Bank and its integration with the National Oncology Network. This initiative connects tumor banks across regional cancer institutes, enabling systematic biological sample collection, particularly from underrepresented populations, such as those with high Amerindian ancestry. Ethical oversight, technical standards, and specialized management software ensure efficient data sharing and genomic research. The network supports cancer research through integration with the Population Cancer Registry, providing unique insights into cancer incidence and outcomes. To date, 5992 cases have been documented. Through international collaboration with Latin American countries, Peru provides a framework for inclusive cancer research, enriching global genomic datasets and strengthening research capacity in diverse and vulnerable populations.

Introduction: Biobanks (BBs) are essential for biomedical research and biodiversity conservation. The Misionero Institute of Biodiversity (IMiBio), located in Misiones, Argentina, is dedicated to preserving the Atlantic Forest through a One Health approach, integrating human, animal, and environmental health. During the COVID-19 pandemic, one of its laboratories was adapted for diagnostic testing, leading to the establishment of a landmark repository of viral extracts of global significance. In addition, IMiBio contributed to the detection of SARS-CoV-2 in wildlife, expanding its BB and strengthening epidemiological surveillance efforts. This growth brought significant challenges in standardization and management. This article examines the institute's evolution, achievements, and post-pandemic perspectives. Materials and Methods: Sample processing is carried out in laboratories corresponding to the specific type of sample received, where they are prepared for entry into the BB. The BB is equipped with -20°C freezers, -80°C ultra-low temperature freezers, and liquid nitrogen tanks to ensure proper preservation of the samples. Results: The BB of IMiBio initially began by storing samples from wild animals obtained through the Güirá Oga Wildlife Rescue Center (GO). Between 2020 and 2024, the BB integrated over 7,696 samples; 43.98% of BB's storage capacity was utilized. The BB now includes RNA from SARS-CoV-2, arboviruses (dengue and chikungunya), respiratory viruses (influenza, respiratory syncytial virus), DNA from human papillomavirus, and tissue samples and microbial isolates from collaborative research. These additions reinforced BB's role in regional epidemiological surveillance but highlighted challenges in maintaining its original biodiversity focus. Conclusions: The IMiBio BB has evolved from a biodiversity repository to include biological samples derived from human diagnostics, particularly SARS-CoV-2, thereby strengthening its role in epidemiological surveillance. However, this expansion necessitates balancing its collections to ensure that its original mission of biodiversity conservation is not compromised. A strategic infrastructure expansion is planned for 2025 to enhance capacity, safety, and services.

Introduction: Incident reporting systems are vital tools for enhancing safety, quality, and continuous improvement in biomedical and health care environments, yet they remain underdeveloped within biobanking, a sector characterized by complexity, high reliability, and multidisciplinary operations. This article addresses the implementation of incident management (IM) and corrective action/preventive action (CAPA) frameworks in biobanks, with a focus on minor- to mid-level incidents and nonconformities. Methods: We conducted a structured literature review using PubMed, Google Scholar, and ResearchGate resources and distinctive keywords or keyword combinations. Relevant articles were screened across biomedical, laboratory safety, and high-reliability domains. In addition, case studies from the literature and operational experiences in biobanks were analyzed, focusing on frequent but underreported incidents. Results: Findings indicate that robust IM and CAPA adoption align with the level of quality management system (QMS) implementation. Case studies highlighted the role of psychosocial factors-such as psychological safety, trust, and nonpunitive reporting-in addition to technical processes like root cause analysis. Effective IM is demonstrated to require more than formal structures; it depends on fostering psychological safety and a trust-based "Restorative Just" culture. Conclusion: We provide the first synthesis of challenges, best practices, and cultural adaptations for IM in biobanking. For the first time, our article provides a thorough synthesis of current challenges, best practices, and cultural adaptations needed to handle incidents, and also a practical toolkit consisting of clear definitions, incident categories, and an implementation guideline to develop efficient nonconformity management in biobanking.

Collaboration between biobanks and research teams is essential for advancing scientific research, particularly in studies involving human subjects; however, various challenges can hinder success. We shed light on key obstacles and challenges encountered during a partnership between a clinical trial and an institutional biobank at a pediatric hospital. In aiming to recruit pediatric biobank participants for a genomic clinical research study, key challenges included low-yield recruitment tactics, independent operations and structures of the biobank and research teams, and transition to a new biobank model resulting in struggles with participant reengagement. This article explores some of the obstacles experienced by the research and biobanks teams, while providing key lessons aimed at guiding others in planning future collaborations for trial recruitment, enrollment, and implementation. We also highlight the significant benefits that can arise when biobanks and research teams work together strategically.

Introduction: Sperm cryopreservation is a vital tool for long-term preservation of genetic material, enabling the maintenance and transfer of genetic traits through assisted reproductive technologies. Objectives: This study aimed to assess the effects of incorporating Lactobacillus plantarum secretions (LS) into the cryopreservation protocol of goat semen. Materials and Methods: LS was added to semen extenders at concentrations of 20, 40, 60, 80, and 100 µL/mL. The control group received no additive. After freezing and thawing, various sperm quality parameters were evaluated. Results: The LS20 group showed significantly higher (p <0.05) total sperm motility compared with LS100, LS80, and the control. Progressive motility and straight-line velocity (VSL) were also improved in LS20 relative to LS100, though not significantly different from the control. LS20 demonstrated significantly higher amplitude of lateral head displacement (ALH) than the control, LS60, LS80, and LS100. LS40 also outperformed LS60-LS100 in ALH. Sperm viability was significantly increased in LS20 and LS40 compared with the control, LS80, and LS100. The sperm chromatin dispersion assay revealed significantly greater halo-to-core ratios in LS20 and LS40. Additionally, malondialdehyde levels, as a marker of oxidative stress, were markedly reduced in LS20 and LS40 compared with all other groups. Conclusions: Lower concentrations of LS, particularly 20 and 40 µL/mL, significantly improve sperm motility, viability, chromatin integrity, and oxidative status after thawing. These findings support the potential application of LS as an effective additive to enhance goat semen cryopreservation outcomes.

Background: The National Cancer Institute's Cancer Moonshot Biobank (CMB) aims to accelerate research on tumor sensitivity and resistance to standard-of-care therapies through collection and distribution of longitudinal biospecimens donated by research participants with cancer. Since low participation among historically underserved populations limits the generalizability of research done with biospecimens, CMB supports local community engagement activities. Objectives: We assessed the factors for enrollment in CMB and related precision oncology trials among rural, low-income adults with cancer who are served by the multi-site MaineHealth Cancer Care Network (MHCCN). We sought to address barriers thus identified. Methods: From October 2021 to May 2022, semi-structured interviews with MHCCN clinical research coordinators (4), oncologists (6), and patients (15) elicited perceived facilitators and barriers to participating in CMB and related trials for low-income rural Mainers. We developed a descriptive model of the steps by which patients become CMB participants based on reports from research coordinators. Factors impacting recruitment were identified at each step. Results: Rural clinics have limited staff to monitor patient lists and collect samples. Many oncologists were skeptical of clinical benefit and correspondingly reluctant to recruit vulnerable patients. Patients were generally open to CMB if recommended by their oncologist but expressed concerns that involvement in CMB or related research would consume limited time, lead to another biopsy, or threaten privacy. Conclusion: Addressing barriers for low-income, rural residents improves access for everyone. To reduce staff burden within a health system, better resourced sites can provide infrastructure and personnel support to sites with fewer resources. Education may correct misunderstandings and improve awareness of the benefits of CMB and related research involvement among research staff, oncologists, and patients.