Biomedical and Environmental Sciences最新文献_第10页

Clinical Evaluation of a Vancomycin Dosage Strategy Based on a Serum Trough Concentration Model in Elderly Patients with Severe Pneumonia 基于血清谷浓度模型的老年重症肺炎万古霉素剂量策略的临床评价

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-05-01 Epub Date: 2023-06-05 DOI: 10.3967/bes2023.049

YAN Wei , SUN Xiao Yan , WANG Meng, ZHAO Fei Fan, ZHOU Qing Tao

Objective

This study aimed to evaluate the clinical benefits of a vancomycin dosage strategy based on a serum trough concentration model in elderly patients.

Methods

This prospective single-center, open-label, randomized controlled trial categorized 66 elderly patients with severe pneumonia into study and control groups. The control group received vancomycin using a regimen decided by the attending physician. Meanwhile, the study group received individualized vancomycin therapy with a dosing strategy based on a serum trough concentration model. The primary endpoint was the proportion of patients with serum trough concentrations reaching the target values. The secondary endpoints were clinical response, vancomycin treatment duration, and vancomycin-associated acute kidney injury (VA-AKI) occurrence.

Results

All patients were at least 60 years old (median age = 81 years). The proportion of patients with target trough concentration achievement (≥ 15 mg/L) with the initial vancomycin regimen was significantly higher in the study group compared to the control group (75.8% vs. 42.4%, P = 0.006). Forty-five patients (68.2%) achieved clinical success, the median duration of vancomycin therapy was 10.0 days, and VA-AKI occurred in eight patients (12.1%). However, there were no significant differences in these parameters between the two groups. The model for predicting vancomycin trough concentrations was upgraded to: serum trough concentration (mg/L) = 17.194 − 0.104 × creatinine clearance rate (mL/min) + 0.313 × vancomycin daily dose [(mg/(kg·d)].

Conclusion

A vancomycin dosage strategy based on a serum trough concentration model can improve the proportion of patients achieving target trough concentrations in elderly patients with severe pneumonia.

目的本研究旨在评估基于血清谷浓度模型的万古霉素剂量策略对老年患者的临床益处。方法本前瞻性单中心、开放标签、随机对照试验将66例老年重症肺炎患者分为研究组和对照组。对照组采用主治医师决定的方案接受万古霉素治疗。同时，研究组接受了基于血清谷浓度模型的个体化万古霉素治疗。主要终点是血清谷浓度达到目标值的患者比例。次要终点是临床反应、万古霉素治疗持续时间和万古霉素相关急性肾损伤（VA-AKI）的发生率。结果所有患者年龄均在60岁以上（中位年龄=81岁）。与对照组相比，研究组首次万古霉素方案达到目标谷浓度（≥15 mg/L）的患者比例显著更高（75.8%对42.4%，P=0.006）。45名患者（68.2%）取得了临床成功，万古霉素治疗的中位持续时间为10.0天，8名患者（12.1%）发生VA-AKI。然而，两组之间的这些参数没有显著差异。预测万古霉素谷浓度的模型升级为：血清谷浓度（mg/L）=17.194−0.104×肌酐清除率（mL/min）+0.313×万古霉素日剂量[（mg/（kg·d）]严重肺炎。

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引用次数: 0

Traditional Chinese Medicine Treatment, Gua Sha, can Induce Subtle Molecular Changes in Gene Expression 中药治疗，瓜沙，可以诱导基因表达的细微分子变化

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-05-01 Epub Date: 2023-06-05 DOI: 10.3967/bes2023.053

QI Fei , CAI Ye , CHEN Jun Jie , CHEN Chun Li , HAN Xue Er , XIA Qiu , KAPRANOV Philipp

Objective

Here, we explored molecular changes that could potentially mediate healing effects of Gua Sha — a method employed by the Chinese traditional medicine with proven track records of safe and efficient applications dating back to ancient times as well as support from randomized controlled trials performed by modern medical studies — yet remaining almost entirely unexplored by the modern-day high-throughput methods of the -omics sciences.

Methods

We investigated transcriptome changes occurring shortly after Gua Sha treatment in the whole blood of healthy volunteers using bulk RNA-seq analysis. We applied various analytical tools to identify genes with consistent expression changes in multiple individuals in response to Gua Sha and their networks.

Results

We found that while the changes were very subtle and individual-specific, we could identify consistent upregulation of three histone genes. Further analysis of the potential regulatory networks of these histone genes revealed the enrichment of functions involved in the immune response and inflammation.

Conclusion

The significance of these results in the context of potential effects of Gua Sha and the next steps in exploring the molecular mechanisms of action of this technique are discussed.

ObjectiveHere，我们探索了可能介导刮痧治疗效果的分子变化。刮痧是中国传统医学使用的一种方法，其安全有效的应用记录可以追溯到古代，并得到了现代医学研究进行的随机对照试验的支持，但现代医学几乎完全没有探索过组学的高通量方法。方法采用批量RNA-seq分析法，研究刮痧治疗后健康志愿者全血中转录组的变化。我们应用各种分析工具来鉴定多个个体对刮痧及其网络的反应中具有一致表达变化的基因。结果我们发现，虽然这些变化非常微妙且具有个体特异性，但我们可以确定三个组蛋白基因的一致上调。对这些组蛋白基因的潜在调控网络的进一步分析揭示了参与免疫反应和炎症的功能的富集。结论这些结果对刮痧潜在作用的意义以及探索该技术作用的分子机制的下一步工作进行了讨论。

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引用次数: 0

Impact of the Early Stage of the COVID-19 Pandemic on Smoking Cessation in Beijing 新冠肺炎疫情早期对北京市戒烟的影响

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-05-01 Epub Date: 2023-06-05 DOI: 10.3967/bes2023.054

WANG Yao, LI Xing Ming

引用次数: 1

Genotyping Characteristics of Human Fecal Escherichia coli and Their Association with Multidrug Resistance in Miyun District, Beijing 北京市密云地区人粪便大肠杆菌基因分型特征及其与多药耐药的关系

IF 3.5 3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-05-01 Epub Date: 2023-06-05 DOI: 10.3967/bes2023.050

ZHANG Wei Wei , ZHU Xiao Lin , DENG Le Le , HAN Ya Jun , LI Zhuo Wei , WANG Jin Long , CHEN Yong Liang , WANG Ao Lin , TIAN Er Li , CHENG Bin , XU Lin Hua , CHEN Yi Cong , TIAN Li Li , HE Guang Xue

Objective

To explore the genotyping characteristics of human fecal Escherichia coli (E. coli) and the relationships between antibiotic resistance genes (ARGs) and multidrug resistance (MDR) of E. coli in Miyun District, Beijing, an area with high incidence of infectious diarrheal cases but no related data.

Methods

Over a period of 3 years, 94 E. coli strains were isolated from fecal samples collected from Miyun District Hospital, a surveillance hospital of the National Pathogen Identification Network. The antibiotic susceptibility of the isolates was determined by the broth microdilution method. ARGs, multilocus sequence typing (MLST), and polymorphism trees were analyzed using whole-genome sequencing data (WGS).

Results

This study revealed that 68.09% of the isolates had MDR, prevalent and distributed in different clades, with a relatively high rate and low pathogenicity. There was no difference in MDR between the diarrheal (49/70) and healthy groups (15/24).

Conclusion

We developed a random forest (RF) prediction model of TEM.1 + baeR + mphA + mphB + QnrS1 + AAC.3-IId to identify MDR status, highlighting its potential for early resistance identification. The causes of MDR are likely mobile units transmitting the ARGs. In the future, we will continue to strengthen the monitoring of ARGs and MDR, and increase the number of strains to further verify the accuracy of the MDR markers.

目的探讨北京市密云区人粪便大肠杆菌（E.coli）的基因分型特征及其与多药耐药性（MDR）的关系。方法在3年的时间里，从国家病原菌鉴定网监测医院密云区医院采集的粪便样本中分离出94株大肠杆菌。采用肉汤微量稀释法测定分离株对抗生素的敏感性。利用全基因组测序数据（WGS）对ARGs、MLST和多态树进行分析。结论我们建立了TEM.1+baeR+mphA+mphB+QnrS1+AAC.3-IId的随机森林（RF）预测模型来识别MDR状态，突出了其早期耐药性鉴定的潜力。MDR的原因可能是传输ARG的移动单元。未来，我们将继续加强对ARGs和MDR的监测，并增加菌株数量，以进一步验证MDR标志物的准确性。

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引用次数: 0

HIV-1 Transmission among Injecting Drug Users is Principally Derived from Local Circulating Strains in Guangxi, China HIV-1在注射吸毒者中的传播主要来源于中国广西当地的流行毒株

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-05-01 Epub Date: 2023-06-05 DOI: 10.3967/bes2023.051

CEN Ping , LIANG Hua Yue , YANG Yuan , ZHANG Fei , YANG Shi Xiong , MO Ju Cong , FENG Yi , HUANG Jie Gang , NING Chuan Yi , HUANG Chun Yuan , YANG Yao , LIANG Na , LIANG Bing Yu , YE Li , LIANG Hao

Objective

The mode of human immunodeficiency virus (HIV) transmission via injection drug use (IDU) still exists, and the recent shift in IDU-related transmission of HIV infection is largely unknown. The purpose of this study was to analyze the spatiotemporal sources and dynamics of HIV-1 transmission through IDU in Guangxi.

Methods

We performed a molecular epidemiological investigation of infections across Guangxi from 2009 to 2019. Phylogenetic and Bayesian time-geographic analyses of HIV-1 sequences were performed to confirm the characteristics of transmission between IDUs in combination with epidemiological data.

Results

Among the 535 subjects, CRF08_BC (57.4%), CRF01_AE (28.4%), and CRF07_BC (10.7%) were the top 3 HIV strains; 72.6% of infections were linked to other provinces in the transmission network; 93.6% of sequence-transmitted strains were locally endemic, with the rest coming from other provinces, predominantly Guangdong and Yunnan; 92.1% of the HIV transmission among people who inject drugs tended to be transmitted between HIV-positive IDUs.

Conclusion

HIV recombinants were high diversity, and circulating local strains were the transmission sources among IDUs in Guangxi. However, there were still cases of IDUs linked to other provinces. Coverage of traditional prevention strategies should be expanded, and inter-provincial collaboration between Guangxi, Yunnan, and Guangdong provinces should be strengthened.

目的人类免疫缺陷病毒（HIV）通过注射吸毒（IDU）传播的模式仍然存在，并且最近与注射吸毒相关的HIV感染传播的转变在很大程度上是未知的。本研究旨在分析广西地区HIV-1通过注射吸毒传播的时空来源和动态。方法对广西2009～2019年感染者进行分子流行病学调查。对HIV-1序列进行系统发育和贝叶斯时间地理分析，结合流行病学数据确认注射吸毒者之间的传播特征。结果535名受试者中，CRF08_BC（57.4%）、CRF01_AE（28.4%）和CRF07_BC（10.7%）为前3名；72.6%的感染与传播网络中的其他省份有关；93.6%的序列传播毒株为本地特有毒株，其余来自其他省份，主要是广东和云南；注射吸毒人群中92.1%的HIV传播倾向于在HIV阳性的注射吸毒者之间传播。结论HIV重组体具有高度的多样性，流行的本地毒株是广西注射吸毒者的传播源。然而，仍有注射吸毒者病例与其他省份有关。应扩大传统预防策略的覆盖范围，并加强广西、云南和广东省之间的省际合作。

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引用次数: 0

A Nested Case-Control Study to Explore the Association between Immunoglobulin G N-glycans and Ischemic Stroke 一项探讨免疫球蛋白G - n -聚糖与缺血性脑卒中关系的巢式病例对照研究

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-05-01 Epub Date: 2023-06-05 DOI: 10.3967/bes2023.048

WANG Bi Yan , SONG Man Shu , ZHANG Jie , MENG Xiao Ni , XING Wei Jia , WANG You Xin

Objective

This study prospectively investigates the association between immunoglobulin G (IgG) N-glycan traits and ischemic stroke (IS) risk.

Methods

A nested case-control study was conducted in the China suboptimal health cohort study, which recruited 4,313 individuals in 2013–2014. Cases were identified as patients diagnosed with IS, and controls were 1:1 matched by age and sex with cases. IgG N-glycans in baseline plasma samples were analyzed.

Results

A total of 99 IS cases and 99 controls were included, and 24 directly measured glycan peaks (GPs) were separated from IgG N-glycans. In directly measured GPs, GP4, GP9, GP21, GP22, GP23, and GP24 were associated with the risk of IS in men after adjusting for age, waist and hip circumference, obesity, diabetes, hypertension, and dyslipidemia. Derived glycan traits representing decreased galactosylation and sialylation were associated with IS in men (FBG2S2/(FBG2 + FBG2S1 + FBG2S2): odds ratio (OR) = 0.92, 95% confidence interval (CI): 0.87–0.97; G1ⁿ: OR = 0.74, 95% CI: 0.63–0.87; G0ⁿ: OR = 1.12, 95% CI: 1.03–1.22). However, these associations were not found among women.

Conclusion

This study validated that altered IgG N-glycan traits were associated with incident IS in men, suggesting that sex discrepancies might exist in these associations.

目的本研究前瞻性地探讨免疫球蛋白G（IgG）N-聚糖特征与缺血性脑卒中（IS）风险之间的关系。方法在中国次优健康队列研究中进行嵌套病例对照研究，该研究在2013-2014年招募了4313名个体。病例被确定为诊断为IS的患者，对照组按年龄和性别与病例1:1匹配。分析基线血浆样品中的IgG N-聚糖。结果共纳入99例IS病例和99例对照组，从IgG N-聚糖中分离出24个直接测量的聚糖峰（GP）。在直接测量的全科医生中，在调整了年龄、腰围和臀围、肥胖、糖尿病、高血压和血脂异常后，GP4、GP9、GP21、GP22、GP23和GP24与男性IS风险相关。代表半乳糖基化和唾液酸化减少的衍生聚糖特征与男性IS相关（FBG2S2/（FBG2+FBG2S1+FBG2S2）：比值比（OR）=0.92，95%置信区间（CI）=0.87–0.97；G1n:OR=0.74，95%置信区间：0.63–0.87；G0n:OR=1.12，95%CI:1.03-1.22）。然而，在女性中没有发现这些关联。结论本研究证实了IgG N-聚糖特征的改变与男性发生IS有关，表明这些相关性中可能存在性别差异。

{"title":"A Nested Case-Control Study to Explore the Association between Immunoglobulin G N-glycans and Ischemic Stroke","authors":"WANG Bi Yan , SONG Man Shu , ZHANG Jie , MENG Xiao Ni , XING Wei Jia , WANG You Xin","doi":"10.3967/bes2023.048","DOIUrl":"10.3967/bes2023.048","url":null,"abstract":"<div><h3>Objective</h3><p>This study prospectively investigates the association between immunoglobulin G (IgG) N-glycan traits and ischemic stroke (IS) risk.</p></div><div><h3>Methods</h3><p>A nested case-control study was conducted in the China suboptimal health cohort study, which recruited 4,313 individuals in 2013–2014. Cases were identified as patients diagnosed with IS, and controls were 1:1 matched by age and sex with cases. IgG N-glycans in baseline plasma samples were analyzed.</p></div><div><h3>Results</h3><p>A total of 99 IS cases and 99 controls were included, and 24 directly measured glycan peaks (GPs) were separated from IgG N-glycans. In directly measured GPs, GP4, GP9, GP21, GP22, GP23, and GP24 were associated with the risk of IS in men after adjusting for age, waist and hip circumference, obesity, diabetes, hypertension, and dyslipidemia. Derived glycan traits representing decreased galactosylation and sialylation were associated with IS in men (FBG2S2/(FBG2 + FBG2S1 + FBG2S2): odds ratio (<em>OR</em>) = 0.92, 95% confidence interval (<em>CI</em>): 0.87–0.97; G1<sup>n</sup>: <em>OR</em> = 0.74, 95% <em>CI</em>: 0.63–0.87; G0<sup>n</sup>: <em>OR</em> = 1.12, 95% <em>CI</em>: 1.03–1.22). However, these associations were not found among women.</p></div><div><h3>Conclusion</h3><p>This study validated that altered IgG N-glycan traits were associated with incident IS in men, suggesting that sex discrepancies might exist in these associations.</p></div>","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 5","pages":"Pages 389-396"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9552731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated Classification of Inherited Retinal Diseases in Optical Coherence Tomography Images Using Few-shot Learning 光学相干断层扫描图像中遗传性视网膜疾病的自动分类

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-05-01 Epub Date: 2023-06-05 DOI: 10.3967/bes2023.052

ZHAO Qi , MAI Si Wei , LI Qian , HUANG Guan Chong , GAO Ming Chen , YANG Wen Li , WANG Ge , MA Ya , LI Lei , PENG Xiao Yan

Objective

To develop a few-shot learning (FSL) approach for classifying optical coherence tomography (OCT) images in patients with inherited retinal disorders (IRDs).

Methods

In this study, an FSL model based on a student–teacher learning framework was designed to classify images. 2,317 images from 189 participants were included. Of these, 1,126 images revealed IRDs, 533 were normal samples, and 658 were control samples.

Results

The FSL model achieved a total accuracy of 0.974–0.983, total sensitivity of 0.934–0.957, total specificity of 0.984–0.990, and total F1 score of 0.935–0.957, which were superior to the total accuracy of the baseline model of 0.943–0.954, total sensitivity of 0.866–0.886, total specificity of 0.962–0.971, and total F1 score of 0.859–0.885. The performance of most subclassifications also exhibited advantages. Moreover, the FSL model had a higher area under curves (AUC) of the receiver operating characteristic (ROC) curves in most subclassifications.

Conclusion

This study demonstrates the effective use of the FSL model for the classification of OCT images from patients with IRDs, normal, and control participants with a smaller volume of data. The general principle and similar network architectures can also be applied to other retinal diseases with a low prevalence.

目的开发一种用于遗传性视网膜疾病（IRD）患者光学相干断层扫描（OCT）图像分类的少数镜头学习（FSL）方法。方法在本研究中，设计了一个基于师生学习框架的FSL模型来对图像进行分类。来自189名参与者的2317张照片被纳入研究。其中1126张图像显示IRD，533张为正常样本，658张为对照样本。结果FSL模型的总准确度为0.974–0.983，总灵敏度为0.934–0.957，总特异度为0.984–0.990，F1总分为0.935–0.957。优于基线模型的总准确性0.943–0.954，总灵敏度0.866–0.886，总特异性0.962–0.971，F1总分0.859–0.885。大多数子类的性能也显示出优势。此外，在大多数子类中，FSL模型的受试者工作特性（ROC）曲线下面积（AUC）更高。结论本研究证明了FSL模型在对IRD患者、正常人和数据量较小的对照参与者的OCT图像进行分类方面的有效应用。一般原理和类似的网络架构也可以应用于其他患病率较低的视网膜疾病。

{"title":"Automated Classification of Inherited Retinal Diseases in Optical Coherence Tomography Images Using Few-shot Learning","authors":"ZHAO Qi , MAI Si Wei , LI Qian , HUANG Guan Chong , GAO Ming Chen , YANG Wen Li , WANG Ge , MA Ya , LI Lei , PENG Xiao Yan","doi":"10.3967/bes2023.052","DOIUrl":"10.3967/bes2023.052","url":null,"abstract":"<div><h3>Objective</h3><p>To develop a few-shot learning (FSL) approach for classifying optical coherence tomography (OCT) images in patients with inherited retinal disorders (IRDs).</p></div><div><h3>Methods</h3><p>In this study, an FSL model based on a student–teacher learning framework was designed to classify images. 2,317 images from 189 participants were included. Of these, 1,126 images revealed IRDs, 533 were normal samples, and 658 were control samples.</p></div><div><h3>Results</h3><p>The FSL model achieved a total accuracy of 0.974–0.983, total sensitivity of 0.934–0.957, total specificity of 0.984–0.990, and total F1 score of 0.935–0.957, which were superior to the total accuracy of the baseline model of 0.943–0.954, total sensitivity of 0.866–0.886, total specificity of 0.962–0.971, and total F1 score of 0.859–0.885. The performance of most subclassifications also exhibited advantages. Moreover, the FSL model had a higher area under curves (AUC) of the receiver operating characteristic (ROC) curves in most subclassifications.</p></div><div><h3>Conclusion</h3><p>This study demonstrates the effective use of the FSL model for the classification of OCT images from patients with IRDs, normal, and control participants with a smaller volume of data. The general principle and similar network architectures can also be applied to other retinal diseases with a low prevalence.</p></div>","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 5","pages":"Pages 431-440"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9552730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seminal Plasma Biochemical Components Mediate the Inverse Association of Microcystin Exposure with Sperm Motility: A Mediation Analysis in Chinese Men 精液生化成分介导微囊藻毒素暴露与精子活力的负相关:中国男性的中介分析

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-05-01 Epub Date: 2023-06-05 DOI: 10.3967/bes2023.056

XU Di Hui , MA Yu Han , YU Wen , ZHOU Xue , LUO Yang , CHEN Ya Bing , HAN Xiao Dong

引用次数: 0

Genotyping Characteristics of Human Fecal Escherichia coli and Their Association with Multidrug Resistance in Miyun District, Beijing 北京市密云区人粪便大肠杆菌的基因分型特征及其与多药耐药性的关系

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-05-01 DOI: 10.3967/bes2023.050

ZHANG Wei Wei , ZHU Xiao Lin , DENG Le Le , HAN Ya Jun , LI Zhuo Wei , WANG Jin Long , CHEN Yong Liang , WANG Ao Lin , TIAN Er Li , CHENG Bin , XU Lin Hua , CHEN Yi Cong , TIAN Li Li , HE Guang Xue

Objective

To explore the genotyping characteristics of human fecal Escherichia coli (E. coli) and the relationships between antibiotic resistance genes (ARGs) and multidrug resistance (MDR) of E. coli in Miyun District, Beijing, an area with high incidence of infectious diarrheal cases but no related data.

Methods

Over a period of 3 years, 94 E. coli strains were isolated from fecal samples collected from Miyun District Hospital, a surveillance hospital of the National Pathogen Identification Network. The antibiotic susceptibility of the isolates was determined by the broth microdilution method. ARGs, multilocus sequence typing (MLST), and polymorphism trees were analyzed using whole-genome sequencing data (WGS).

Results

This study revealed that 68.09% of the isolates had MDR, prevalent and distributed in different clades, with a relatively high rate and low pathogenicity. There was no difference in MDR between the diarrheal (49/70) and healthy groups (15/24).

Conclusion

We developed a random forest (RF) prediction model of TEM.1 + baeR + mphA + mphB + QnrS1 + AAC.3-IId to identify MDR status, highlighting its potential for early resistance identification. The causes of MDR are likely mobile units transmitting the ARGs. In the future, we will continue to strengthen the monitoring of ARGs and MDR, and increase the number of strains to further verify the accuracy of the MDR markers.

目的探讨北京市密云区人粪便大肠杆菌（E.coli）的基因分型特征及其与多药耐药性（MDR）的关系。方法在3年的时间里，从国家病原菌鉴定网监测医院密云区医院采集的粪便样本中分离出94株大肠杆菌。采用肉汤微量稀释法测定分离株对抗生素的敏感性。利用全基因组测序数据（WGS）对ARGs、MLST和多态树进行分析。结论我们建立了TEM.1+baeR+mphA+mphB+QnrS1+AAC.3-IId的随机森林（RF）预测模型来识别MDR状态，突出了其早期耐药性鉴定的潜力。MDR的原因可能是传输ARG的移动单元。未来，我们将继续加强对ARGs和MDR的监测，并增加菌株数量，以进一步验证MDR标志物的准确性。

{"title":"Genotyping Characteristics of Human Fecal Escherichia coli and Their Association with Multidrug Resistance in Miyun District, Beijing","authors":"ZHANG Wei Wei , ZHU Xiao Lin , DENG Le Le , HAN Ya Jun , LI Zhuo Wei , WANG Jin Long , CHEN Yong Liang , WANG Ao Lin , TIAN Er Li , CHENG Bin , XU Lin Hua , CHEN Yi Cong , TIAN Li Li , HE Guang Xue","doi":"10.3967/bes2023.050","DOIUrl":"https://doi.org/10.3967/bes2023.050","url":null,"abstract":"<div><h3>Objective</h3><p>To explore the genotyping characteristics of human fecal <em>Escherichia coli</em> (<em>E. coli</em>) and the relationships between antibiotic resistance genes (ARGs) and multidrug resistance (MDR) of <em>E. coli</em> in Miyun District, Beijing, an area with high incidence of infectious diarrheal cases but no related data.</p></div><div><h3>Methods</h3><p>Over a period of 3 years, 94 <em>E. coli</em> strains were isolated from fecal samples collected from Miyun District Hospital, a surveillance hospital of the National Pathogen Identification Network. The antibiotic susceptibility of the isolates was determined by the broth microdilution method. ARGs, multilocus sequence typing (MLST), and polymorphism trees were analyzed using whole-genome sequencing data (WGS).</p></div><div><h3>Results</h3><p>This study revealed that 68.09% of the isolates had MDR, prevalent and distributed in different clades, with a relatively high rate and low pathogenicity. There was no difference in MDR between the diarrheal (49/70) and healthy groups (15/24).</p></div><div><h3>Conclusion</h3><p>We developed a random forest (RF) prediction model of <em>TEM.1 + baeR + mphA + mphB + QnrS1 + AAC.3-IId</em> to identify MDR status, highlighting its potential for early resistance identification. The causes of MDR are likely mobile units transmitting the ARGs. In the future, we will continue to strengthen the monitoring of ARGs and MDR, and increase the number of strains to further verify the accuracy of the MDR markers.</p></div>","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 5","pages":"Pages 406-417"},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49843900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fine Particulate Matter Exposure Induces Toxicity by Regulating Nuclear Factor-κB/toll-like Receptor 4/myeloid Differentiation Primary Response Signaling Pathways in RAW264.7 Cells 细颗粒物暴露通过调节核因子-κB/toll样受体4/髓样分化初级反应信号通路诱导RAW264.7细胞毒性

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-05-01 Epub Date: 2023-06-05 DOI: 10.3967/bes2023.055

ZHENG Mei Zhu , LU Yao , LU Ting Ting , QIN Peng , LI Yu Qiu , SHI Dong Fang

引用次数: 0