Cai Hong YIN , Zhan Yun SONG , Xing Xing LIU , Xiao Mu WANG , Ying WANG , Cheng Cheng GAO , Xiu Ling SONG , Xin FENG
{"title":"Comparison of Microdroplet Digital PCR Assays with Real-time Fluorescence Quantitative PCR for Clostridioides difficile Detection","authors":"Cai Hong YIN , Zhan Yun SONG , Xing Xing LIU , Xiao Mu WANG , Ying WANG , Cheng Cheng GAO , Xiu Ling SONG , Xin FENG","doi":"10.3967/bes2023.094","DOIUrl":"https://doi.org/10.3967/bes2023.094","url":null,"abstract":"","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 7","pages":"Pages 653-657"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49838908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiao Li CHEN , Hao LIANG , Peng Bo GUO , Yi Xin GU , Jia Qi WANG , Hai Rui WANG , Gui Lan ZHOU , Zhu Jun SHAO , Jian Zhong ZHANG , Mao Jun ZHANG
Objective
Campylobacter jejuni NCTC11168 is commonly used as a standard strain for flagellar biosynthesis research. In this report, two distinguished phenotypic isolates (CJ1Z, flhA mutant strain, lawn; CJ2S, flhA complemented strain, normal colony) appeared during laboratory passages for NCTC11168.
Methods
Phenotypic assessments, including motility plates, transmission electron microscopy, biofilm formation assay, autoagglutination assay, and genome re-sequencing for these two isolates (CJ1Z, flhA mutant strain; CJ2S, flhA complemented strain) were carried out in this study.
Results
Transmission electron microscopy revealed that the flagellum was lost in CJ1Z. Phenotypic assessments and genome sequencing of the two isolates were performed in this study. The capacity for biofilm formation, colony auto-agglutination, and isolate motility was reduced in the mutant CJ1Z. Comparative genomic analysis indicated a unique native nucleotide insertion in flhA (nt, 2154) that caused the I719Y and I720Y mutations and early truncation in flhA.
Conclusion
FlhA has been found to influence the expression of flagella in C. jejuni. To the best of our knowledge, this is the first study to describe the function of the C-terminal of this protein.
{"title":"Genetic and Phenotypic Variation of Campylobacter jejuni NCTC11168 Caused by flhA Mutation during Laboratory Passage","authors":"Xiao Li CHEN , Hao LIANG , Peng Bo GUO , Yi Xin GU , Jia Qi WANG , Hai Rui WANG , Gui Lan ZHOU , Zhu Jun SHAO , Jian Zhong ZHANG , Mao Jun ZHANG","doi":"10.3967/bes2023.088","DOIUrl":"https://doi.org/10.3967/bes2023.088","url":null,"abstract":"<div><h3>Objective</h3><p><em>Campylobacter jejuni</em> NCTC11168 is commonly used as a standard strain for flagellar biosynthesis research. In this report, two distinguished phenotypic isolates (CJ1Z, <em>flhA</em> mutant strain, lawn; CJ2S, <em>flhA</em> complemented strain, normal colony) appeared during laboratory passages for NCTC11168.</p></div><div><h3>Methods</h3><p>Phenotypic assessments, including motility plates, transmission electron microscopy, biofilm formation assay, autoagglutination assay, and genome re-sequencing for these two isolates (CJ1Z, <em>flhA</em> mutant strain; CJ2S, <em>flhA</em> complemented strain) were carried out in this study.</p></div><div><h3>Results</h3><p>Transmission electron microscopy revealed that the flagellum was lost in CJ1Z. Phenotypic assessments and genome sequencing of the two isolates were performed in this study. The capacity for biofilm formation, colony auto-agglutination, and isolate motility was reduced in the mutant CJ1Z. Comparative genomic analysis indicated a unique native nucleotide insertion in flhA (nt, 2154) that caused the I719Y and I720Y mutations and early truncation in flhA.</p></div><div><h3>Conclusion</h3><p>FlhA has been found to influence the expression of flagella in <em>C. jejuni</em>. To the best of our knowledge, this is the first study to describe the function of the C-terminal of this protein.</p></div>","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 7","pages":"Pages 604-613"},"PeriodicalIF":3.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49727465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yi Ru CHEN , Yue Ting LI , Yi Fei CHEN , Jia Yu GONG , Jing Yu YANG , Hai Tao YU , Wen Hui XU , Chun Ji PIAO , Lin XIE
{"title":"Pre-pregnant Overweight Interacts with ADIPOQ Genetic Variants to Influence Polyunsaturated Fatty Acids in Human Milk","authors":"Yi Ru CHEN , Yue Ting LI , Yi Fei CHEN , Jia Yu GONG , Jing Yu YANG , Hai Tao YU , Wen Hui XU , Chun Ji PIAO , Lin XIE","doi":"10.3967/bes2023.090","DOIUrl":"https://doi.org/10.3967/bes2023.090","url":null,"abstract":"","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 7","pages":"Pages 635-638"},"PeriodicalIF":3.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49727466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ying TIAN , Hong Peng CAO , Yu Ping HUAN , Jia Wei GONG , Kai Hua YUAN , Wen Zhuo CHEN , Jing HU , Yu Fei SHI
Objective
To determine the thermic effect of food (TEF) in a Chinese mixed diet in young people.
Methods
During the study, the participants were weighed and examined for body composition every morning. The total energy expenditure (TEE) of the participants was measured by the doubly labelled water method for 7 days, and during this period, basal energy expenditure was measured by indirect calorimetry and physical activity energy expenditure was measured by an accelerometer. The value obtained by subtracting basal energy expenditure and physical activity energy expenditure from TEE was used to calculate TEF.
Results
Twenty healthy young students (18–30 years; 10 male) participated in the study. The energy intake of the participants was not significantly different from the Chinese Dietary Reference Intake of energy (P > 0.05). The percentage of energy from protein, fat and carbohydrate were all in the normal range. The intakes of fruits, milk and dietary fiber of the participants were significantly lower than those in the Chinese Dietary Guidelines (P < 0.05). There was no significant difference in the body weight of the participants during the experiment (P > 0.05). When adjusted for body weight, there was no significant difference in either TEE or basal energy expenditure between the male and female participants (P > 0.05). In addition, there was no significant difference in physical activity energy expenditure and TEF between the male and female participants (P > 0.05). The percentage of TEF in TEE was 8.73%.
Conclusion
The percentage of TEF in TEE in a Chinese mixed diet in young people was significantly lower than 10% (P < 0.001). A value of 10% is usually considered to be the TEF in mixed diets as a percentage of TEE.
{"title":"Measurement of the Thermic Effect of Food in a Chinese Mixed Diet in Young People","authors":"Ying TIAN , Hong Peng CAO , Yu Ping HUAN , Jia Wei GONG , Kai Hua YUAN , Wen Zhuo CHEN , Jing HU , Yu Fei SHI","doi":"10.3967/bes2023.086","DOIUrl":"10.3967/bes2023.086","url":null,"abstract":"<div><h3>Objective</h3><p>To determine the thermic effect of food (TEF) in a Chinese mixed diet in young people.</p></div><div><h3>Methods</h3><p>During the study, the participants were weighed and examined for body composition every morning. The total energy expenditure (TEE) of the participants was measured by the doubly labelled water method for 7 days, and during this period, basal energy expenditure was measured by indirect calorimetry and physical activity energy expenditure was measured by an accelerometer. The value obtained by subtracting basal energy expenditure and physical activity energy expenditure from TEE was used to calculate TEF.</p></div><div><h3>Results</h3><p>Twenty healthy young students (18–30 years; 10 male) participated in the study. The energy intake of the participants was not significantly different from the Chinese Dietary Reference Intake of energy (<em>P</em> > 0.05). The percentage of energy from protein, fat and carbohydrate were all in the normal range. The intakes of fruits, milk and dietary fiber of the participants were significantly lower than those in the Chinese Dietary Guidelines (<em>P</em> < 0.05). There was no significant difference in the body weight of the participants during the experiment (<em>P</em> > 0.05). When adjusted for body weight, there was no significant difference in either TEE or basal energy expenditure between the male and female participants (<em>P</em> > 0.05). In addition, there was no significant difference in physical activity energy expenditure and TEF between the male and female participants (<em>P</em> > 0.05). The percentage of TEF in TEE was 8.73%.</p></div><div><h3>Conclusion</h3><p>The percentage of TEF in TEE in a Chinese mixed diet in young people was significantly lower than 10% (<em>P</em> < 0.001). A value of 10% is usually considered to be the TEF in mixed diets as a percentage of TEE.</p></div>","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 7","pages":"Pages 585-594"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9923851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hui LIU , Xin WANG , Peng WANG , Yu Qi WANG , Yun Yun YI , Xin LI
{"title":"Novel Protein C6ORF120 Promotes Apoptosis through Mitochondria-dependent Pathway in CD4+T Lymphocytes","authors":"Hui LIU , Xin WANG , Peng WANG , Yu Qi WANG , Yun Yun YI , Xin LI","doi":"10.3967/bes2023.091","DOIUrl":"https://doi.org/10.3967/bes2023.091","url":null,"abstract":"","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 7","pages":"Pages 639-643"},"PeriodicalIF":3.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49767404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Growth Standard for Children Under 7 Years of Age","authors":"","doi":"10.3967/bes2023.096","DOIUrl":"10.3967/bes2023.096","url":null,"abstract":"","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 7","pages":"Pages 663-664"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9923858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Standard for Healthy Chinese Older Adults","authors":"","doi":"10.3967/bes2023.098","DOIUrl":"10.3967/bes2023.098","url":null,"abstract":"","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 7","pages":"Pages 666-667"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9923857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Rapid Trace Analysis of GBS using the Specific Spectral Absorption of Chromogenic Culture Media","authors":"Yang CHEN , Yan Yan ZHANG , Yao Gen SHU","doi":"10.3967/bes2023.093","DOIUrl":"10.3967/bes2023.093","url":null,"abstract":"","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 7","pages":"Pages 649-652"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9923862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiao Li CHEN , Hao LIANG , Peng Bo GUO , Yi Xin GU , Jia Qi WANG , Hai Rui WANG , Gui Lan ZHOU , Zhu Jun SHAO , Jian Zhong ZHANG , Mao Jun ZHANG
Objective
Campylobacter jejuni NCTC11168 is commonly used as a standard strain for flagellar biosynthesis research. In this report, two distinguished phenotypic isolates (CJ1Z, flhA mutant strain, lawn; CJ2S, flhA complemented strain, normal colony) appeared during laboratory passages for NCTC11168.
Methods
Phenotypic assessments, including motility plates, transmission electron microscopy, biofilm formation assay, autoagglutination assay, and genome re-sequencing for these two isolates (CJ1Z, flhA mutant strain; CJ2S, flhA complemented strain) were carried out in this study.
Results
Transmission electron microscopy revealed that the flagellum was lost in CJ1Z. Phenotypic assessments and genome sequencing of the two isolates were performed in this study. The capacity for biofilm formation, colony auto-agglutination, and isolate motility was reduced in the mutant CJ1Z. Comparative genomic analysis indicated a unique native nucleotide insertion in flhA (nt, 2154) that caused the I719Y and I720Y mutations and early truncation in flhA.
Conclusion
FlhA has been found to influence the expression of flagella in C. jejuni. To the best of our knowledge, this is the first study to describe the function of the C-terminal of this protein.
{"title":"Genetic and Phenotypic Variation of Campylobacter jejuni NCTC11168 Caused by flhA Mutation during Laboratory Passage","authors":"Xiao Li CHEN , Hao LIANG , Peng Bo GUO , Yi Xin GU , Jia Qi WANG , Hai Rui WANG , Gui Lan ZHOU , Zhu Jun SHAO , Jian Zhong ZHANG , Mao Jun ZHANG","doi":"10.3967/bes2023.088","DOIUrl":"https://doi.org/10.3967/bes2023.088","url":null,"abstract":"<div><h3>Objective</h3><p><em>Campylobacter jejuni</em> NCTC11168 is commonly used as a standard strain for flagellar biosynthesis research. In this report, two distinguished phenotypic isolates (CJ1Z, <em>flhA</em> mutant strain, lawn; CJ2S, <em>flhA</em> complemented strain, normal colony) appeared during laboratory passages for NCTC11168.</p></div><div><h3>Methods</h3><p>Phenotypic assessments, including motility plates, transmission electron microscopy, biofilm formation assay, autoagglutination assay, and genome re-sequencing for these two isolates (CJ1Z, <em>flhA</em> mutant strain; CJ2S, <em>flhA</em> complemented strain) were carried out in this study.</p></div><div><h3>Results</h3><p>Transmission electron microscopy revealed that the flagellum was lost in CJ1Z. Phenotypic assessments and genome sequencing of the two isolates were performed in this study. The capacity for biofilm formation, colony auto-agglutination, and isolate motility was reduced in the mutant CJ1Z. Comparative genomic analysis indicated a unique native nucleotide insertion in flhA (nt, 2154) that caused the I719Y and I720Y mutations and early truncation in flhA.</p></div><div><h3>Conclusion</h3><p>FlhA has been found to influence the expression of flagella in <em>C. jejuni</em>. To the best of our knowledge, this is the first study to describe the function of the C-terminal of this protein.</p></div>","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 7","pages":"Pages 604-613"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49838907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhi Chang ZHENG , Wei YUAN , Nian WANG , Bo JIANG , Chun Peng MA , Hui AI , Xiao WANG , Shao Ping NIE
Objective
We aimed to assess the feasibility and superiority of machine learning (ML) methods to predict the risk of Major Adverse Cardiovascular Events (MACEs) in chest pain patients with NSTE-ACS.
Methods
Enrolled chest pain patients were from two centers, Beijing Anzhen Emergency Chest Pain Center Beijing Bo’ai Hospital, China Rehabilitation Research Center. Five classifiers were used to develop ML models. Accuracy, Precision, Recall, F-Measure and AUC were used to assess the model performance and prediction effect compared with HEART risk scoring system. Ultimately, ML model constructed by Naïve Bayes was employed to predict the occurrence of MACEs.
Results
According to learning metrics, ML models constructed by different classifiers were superior over HEART (History, ECG, Age, Risk factors, & Troponin) scoring system when predicting acute myocardial infarction (AMI) and all-cause death. However, according to ROC curves and AUC, ML model constructed by different classifiers performed better than HEART scoring system only in prediction for AMI. Among the five ML algorithms, Linear support vector machine (SVC), Naïve Bayes and Logistic regression classifiers stood out with all Accuracy, Precision, Recall and F-Measure from 0.8 to 1.0 for predicting any event, AMI, revascularization and all-cause death (vs. HEART ≤ 0.78), with AUC from 0.88 to 0.98 for predicting any event, AMI and revascularization (vs. HEART ≤ 0.85). ML model developed by Naïve Bayes predicted that suspected acute coronary syndrome (ACS), abnormal electrocardiogram (ECG), elevated hs-cTn I, sex and smoking were risk factors of MACEs.
Conclusion
Compared with HEART risk scoring system, the superiority of ML method was demonstrated when employing Linear SVC classifier, Naïve Bayes and Logistic. ML method could be a promising method to predict MACEs in chest pain patients with NSTE-ACS.
{"title":"Exploring the Feasibility of Machine Learning to Predict Risk Stratification Within 3 Months in Chest Pain Patients with Suspected NSTE-ACS","authors":"Zhi Chang ZHENG , Wei YUAN , Nian WANG , Bo JIANG , Chun Peng MA , Hui AI , Xiao WANG , Shao Ping NIE","doi":"10.3967/bes2023.089","DOIUrl":"10.3967/bes2023.089","url":null,"abstract":"<div><h3>Objective</h3><p>We aimed to assess the feasibility and superiority of machine learning (ML) methods to predict the risk of Major Adverse Cardiovascular Events (MACEs) in chest pain patients with NSTE-ACS.</p></div><div><h3>Methods</h3><p>Enrolled chest pain patients were from two centers, Beijing Anzhen Emergency Chest Pain Center Beijing Bo’ai Hospital, China Rehabilitation Research Center. Five classifiers were used to develop ML models. Accuracy, Precision, Recall, F-Measure and AUC were used to assess the model performance and prediction effect compared with HEART risk scoring system. Ultimately, ML model constructed by Naïve Bayes was employed to predict the occurrence of MACEs.</p></div><div><h3>Results</h3><p>According to learning metrics, ML models constructed by different classifiers were superior over HEART (History, ECG, Age, Risk factors, & Troponin) scoring system when predicting acute myocardial infarction (AMI) and all-cause death. However, according to ROC curves and AUC, ML model constructed by different classifiers performed better than HEART scoring system only in prediction for AMI. Among the five ML algorithms, Linear support vector machine (SVC), Naïve Bayes and Logistic regression classifiers stood out with all Accuracy, Precision, Recall and F-Measure from 0.8 to 1.0 for predicting any event, AMI, revascularization and all-cause death (<em>vs.</em> HEART ≤ 0.78), with AUC from 0.88 to 0.98 for predicting any event, AMI and revascularization (<em>vs.</em> HEART ≤ 0.85). ML model developed by Naïve Bayes predicted that suspected acute coronary syndrome (ACS), abnormal electrocardiogram (ECG), elevated hs-cTn I, sex and smoking were risk factors of MACEs.</p></div><div><h3>Conclusion</h3><p>Compared with HEART risk scoring system, the superiority of ML method was demonstrated when employing Linear SVC classifier, Naïve Bayes and Logistic. ML method could be a promising method to predict MACEs in chest pain patients with NSTE-ACS.</p></div>","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 7","pages":"Pages 625-634"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9929826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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