Biomedical and Environmental Sciences最新文献

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Machine-learning-assisted Investigation into the Relationship between the Built Environment, Behavior, and Physical Health of the Elderly in China 机器学习辅助调查中国老年人的建筑环境、行为和身体健康之间的关系。

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-10-01 DOI: 10.3967/bes2023.125

Xiao Ping WANG , Ze Yan LI , Meng ZHANG , Hong Yong LIU

引用次数: 0

Role of Prognostic Marker PRR11 in Immune Infiltration for Facilitating Lung Adenocarcinoma Progression 预后标志物PRR11在免疫浸润促进肺腺癌进展中的作用

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-09-01 DOI: 10.3967/bes2023.111

Wen Hao WANG , Chang Geng MA , Yun Shang CUI , Bing Yu BAI , Zhi Mei SHENG , Jin LIU , Ao LI , Bao Gang ZHANG

引用次数: 0

The Link between Exposure to Phthalates and Type 2 Diabetes Mellitus: A Study Based on NHANES Data and Bioinformatic Analysis 邻苯二甲酸盐暴露与2型糖尿病之间的联系：基于NHANES数据和生物信息学分析的研究。

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-09-01 DOI: 10.3967/bes2023.115

Xue Kui LIU , Shan Wen SI , Yan YE , Jia Yi LI , He He LYU , Ya Mei MA , Cai Yan ZOU , Hao Jie SUN , Lei XUE , Wei XU , Hou Fa GENG , Jun LIANG

Insulin resistance is closely associated with metabolic disorders and the pathogenesis of type 2 diabetes mellitus (T2DM). Accumulating evidence suggests that excess fatty acids due to inappropriate lipolysis increase the risk of insulin resistance, which, in turn, stimulates hyperinsulinemia, worsens metabolism

引用次数: 1

Establishment and Modification of Ninety-seven Pneumococcal Serotyping Assays Based on Quantitative Real-time Polymerase Chain Reaction 基于定量实时聚合酶链反应的97种肺炎球菌血清学分型方法的建立和改进。

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-09-01 DOI: 10.3967/bes2023.078

Jie CHE , Bo Han CHEN , Li XU , Yuan GAO , Meng Meng YUE , Zi Man CHEN , Mao Jun ZHANG , Zhu Jun SHAO

Objective

To establish and modify quantitative real-time polymerase chain reaction (qPCR)-based serotyping assays to distinguish 97 pneumococcal serotypes.

Methods

A database of capsular polysaccharide (cps) loci sequences was generated, covering 97 pneumococcal serotypes. Bioinformatics analyses were performed to identify the cps loci structure and target genes related to different pneumococcal serotypes with specific SNPs. A total of 27 novel qPCR serotyping assay primers and probes were established based on qPCR, while 27 recombinant plasmids containing serotype-specific DNA sequence fragments were constructed as reference target sequences to examine the specificity and sensitivity of the qPCR assay. A panel of pneumococcal reference strains was employed to evaluate the capability of pneumococcal serotyping.

Results

A total of 97 pneumococcal serotyping assays based on qPCR were established and modified, which included 64 serotypes previously reported as well as an additional 33 serotypes. Twenty-seven novel qPCR serotyping target sequences were implemented in the pneumococcal qPCR serotyping system. A total of 97 pneumococcal serotypes, which included 52 individual serotypes and 45 serotypes belonging to 20 serogroups, could not be identified as individual serotypes. The sensitivity of qPCR assays based on 27 target sequences was 1–100 copies/μL. The specificity of the qPCR assays was 100%, which were tested by a panel of 90 serotypes of the pneumococcal reference strains.

Conclusion

A total of 27 novel qPCR assays were established and modified to analyze 97 pneumococcal serotypes.

目的：建立和改进基于定量实时聚合酶链式反应（qPCR）的血清分型方法，以区分97种肺炎球菌血清型。方法：建立97种肺炎球菌血清型荚膜多糖（cps）基因座序列数据库。进行生物信息学分析，以确定与具有特定SNPs的不同肺炎球菌血清型相关的cps基因座结构和靶基因。在qPCR的基础上，共建立了27个新的qPCR血清分型检测引物和探针，并构建了27个含有血清型特异性DNA序列片段的重组质粒作为参考靶序列，以检测qPCR检测的特异性和敏感性。采用一组肺炎球菌参考菌株来评估肺炎球菌血清分型的能力。结果：共建立并改进了97种基于qPCR的肺炎球菌血清分型方法，其中包括先前报道的64种血清型以及另外33种血清型。在肺炎球菌qPCR血清学分型系统中实现了27个新的qPCR血清学靶序列。共有97种肺炎球菌血清型，包括52种单独血清型和属于20个血清群的45种血清型，无法确定为单独血清型。基于27个靶序列的qPCR测定的灵敏度为1-100拷贝/µL。qPCR检测的特异性为100%，由90种血清型肺炎球菌参考菌株组成的小组进行了测试。结论：共建立并改进了27种新的qPCR检测方法，可分析97种肺炎球菌血清型。

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引用次数: 0

Role of Prognostic Marker PRR11 in Immune Infiltration for Facilitating Lung Adenocarcinoma Progression 预后标志物PRR11在免疫浸润促进肺腺癌进展中的作用

IF 3.5 3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-09-01 DOI: 10.3967/bes2023.111

Wen Hao WANG , Chang Geng MA , Yun Shang CUI , Bing Yu BAI , Zhi Mei SHENG , Jin LIU , Ao LI , Bao Gang ZHANG

The PRR11 gene (Proline Rich 11) has been implicated in lung cancer; however, relationship between PRR11 and immune infiltration is not clearly understood. In this study, we used The Cancer Genome Atlas (TCGA) data to analyze the lung adenocarcinoma patients; PRR11 gene expression, clinicopathological findings, enrichment, and immune infiltration were also studied. PRR11 immune response expression assays in lung adenocarcinoma (LUAD) were performed using TIMER, and statistical analysis and visualization were conducted using R software. All data were verified using Gene Expression Profiling Interactive Analysis (GEPIA), and the Human Protein Atlas (HPA). We found that PRR11 was an important prognostic factor in patients with LUAD. PRR11 expression was correlated with tumor stage and progression. Gene Set Enrichment Analysis (GSEA) showed that PRR11 was enriched in the cell cycle regulatory pathways. Immune infiltration analysis revealed that the number of T helper 2 (Th2) cells increased when PRR11 was overexpressed. These results confirm the role of PRR11 as a prognostic marker of lung adenocarcinoma by controlling the cell cycle and influencing the immune system to facilitate lung cancer progression.

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引用次数: 0

Nutrition Guidelines of Complementary Feeding for Infants and Toddlers (WS/T 678—2020) WS-T 678-2020《婴幼儿补充喂养营养指南》。

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-09-01 DOI: 10.3967/bes2023.117

引用次数: 2

Epidemiological and Clinical Characteristics of Non-neonatal Tetanus Patients in Guangxi, China: An 11-year Retrospective Study (2011–2021) 广西非新生儿破伤风患者的流行病学和临床特征：11年回顾性研究（2011-2021）。

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-09-01 DOI: 10.3967/bes2023.113

Yi Wen KANG , Guo Feng MAI , Xiao Ling ZHU , Shang Qin DENG , Shi Xiong YANG , Hong Li TENG , Zong Xiang YUAN , Chu Ye MO , Jian Yan LIN , Li YE , Hua Min TANG

引用次数: 0

Characterization of Mutations in Genes Related to Rifampicin and Isoniazid Resistance in Multidrug-resistant Mycobacterium tuberculosis Strains from Hangzhou, China 杭州地区多药耐药结核分枝杆菌耐利福平和异烟肼相关基因突变的研究

3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-09-01 DOI: 10.3967/bes2023.112

Yin Yan HUANG, Li XIE, Yi Fei WU, Qing Jun JIA, Qing Lin CHENG, Qing Chun LI, Li Yun AI, Xue Xin BAI

引用次数: 0

Characterization of Mutations in Genes Related to Rifampicin and Isoniazid Resistance in Multidrug-resistant Mycobacterium tuberculosis Strains from Hangzhou, China 杭州耐多药结核分枝杆菌利福平和异烟肼耐药性相关基因突变特征

IF 3.5 3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-09-01 DOI: 10.3967/bes2023.112

Yin Yan HUANG, Li XIE, Yi Fei WU, Qing Jun JIA, Qing Lin CHENG, Qing Chun LI, Li Yun AI, Xue Xin BAI

引用次数: 0

An Investigation of the Effects of B7-H4 Gene rs10754339 and miR-125a Gene rs12976445 on Cancer Susceptibility B7-H4基因rs10754339和miR-125a基因rs12976445对肿瘤易感性影响的研究

IF 3.5 3区医学 Q2 ENVIRONMENTAL SCIENCES

Biomedical and Environmental Sciences

Pub Date : 2023-09-01 DOI: 10.3967/bes2023.074

Yu Chen JIN , Li Juan DONG , Qin Yue YANG , Wei Ning XIONG , Wei Yi WANG , Xian Hong FENG , Wei YU , Wei HUANG , Bi Feng CHEN

Objective

To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.

Methods

A total of 1,490 cancer patients (lung/gastric/liver/: 550/460/480) and 800 controls were recruited in this case-control study. The meta-analysis was performed by pooling the data from previous related studies and the present study.

Results

The results of this study showed that in the Hubei Han Chinese population, the rs10754339 gene was significantly associated with the risk of lung and gastric cancer but not liver cancer, and the rs12976445 gene was significantly associated with the risk of lung cancer but not liver or gastric cancer. The meta-analysis results indicated that rs10754339 and rs12976445 contributed to cancer susceptibility in the Chinese population and also revealed a significant association between rs10754339 and breast cancer risk, as well as between rs12976445 and lung cancer risk.

Conclusion

The B7-H4 gene rs10754339 and miR-125a gene rs12976445 may be the potential genetic markers for cancer susceptibility in the Chinese population, which should be validated in future studies with larger sample sizes in other ethnic populations.

目的通过病例对照和荟萃分析研究B7-H4基因rs10754339和miR-125a基因rs12976445对癌症易感性的影响。方法对1490例癌症患者（肺/胃/肝/：550/460/480）和800例对照者进行病例对照研究。荟萃分析是通过汇集先前相关研究和本研究的数据进行的。结果在湖北汉族人群中，rs10754339基因与肺癌和胃癌癌症风险显著相关，但与癌症风险无关，rs12976445基因与肺癌或胃癌风险显著相关。荟萃分析结果表明，rs10754339和rs12976445对中国人群癌症易感性有贡献，并揭示了rs10754338与癌症风险以及rs1297645与癌症风险之间的显著关联。结论B7-H4基因rs10754339和miR-125a基因rs12976445可能是中国人群癌症易感性的潜在遗传标志物，这一点应在未来的研究中得到验证，在其他民族人群中进行更大的样本量。

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引用次数: 0

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