Biological Bulletin最新文献

AbstractClimate change threatens biodiversity worldwide, and assessing how those changes will impact communities will be critical for conservation. Dominant primary producers can alter local-scale environmental conditions, reducing temperature via shading and mitigating ocean acidification via photosynthesis, which could buffer communities from the impacts of climate change. We conducted two experiments on the coast of southeastern Alaska to assess the effects of a common seaweed species, Neorhodomela oregona, on temperature and pH in field tide pools and tide pool mesocosms. We found that N. oregona was numerically dominant in this system, covering >60% of habitable space in the pools and accounting for >40% of live cover. However, while N. oregona had a density-dependent effect on pH in isolated mesocosms, we did not find a consistent effect of N. oregona on either pH or water temperature in tide pools in the field. These results suggest that the amelioration of climate change impacts in immersed marine ecosystems by primary producers is not universal and likely depends on species' functional attributes, including photosynthetic rate and physical structure, in addition to abundance or dominance.

AbstractIn brittle stars, the coelomic cavities that form during embryogenesis contribute to most of the internal organ systems of the juvenile. In the ancestral mode of development, the coelomic cavities begin with bilateral symmetry and play a minor role in the function of the ophiopluteus larva. However, the coelomic cavities undergo extensive changes during metamorphosis to set up the body systems of the juvenile brittle star. Many lineages of brittle stars have evolved life histories without the ophiopluteus larva. The non-feeding vitellaria larva has rapid development of juvenile structures. This work demonstrates the modifications to the origin and early development of the coelomic cavities in a vitellaria larva. Much of the archenteron forms an unpaired axocoel, hydrocoel, and somatocoel. The posterior-most portion of the archenteron forms the rudiment of the juvenile stomach. The right somatocoel and a portion of the left somatocoel form as invaginations of the lateral ectoderm. Later morphogenesis of the axocoel, the hydrocoel, and the two somatocoels is similar to what has been shown for brittle stars with an ophiopluteus larva. Confocal microscopy and three-dimensional modeling were used to show new details for the later morphogenesis of the coelomic cavities. The stone canal originates as an outgrowth of the hydrocoel between lobes 4 and 5. The hydrocoel lobes have minimal migration after they meet to complete the ring canal. The right somatocoel contributes to a component of the axial complex and perihemal system. A detailed description is given for how the left somatocoel contributes to multiple organ systems.

AbstractThe temperature-size rule is one of the universal rules in ecology and states that ectotherms in warmer waters will grow faster as juveniles, mature at smaller sizes and younger ages, and reach smaller maximum body sizes. Many models have unsuccessfully attempted to reproduce temperature-size rule-consistent life histories by using two-term (anabolism and catabolism) Pütter-type growth models, such as the von Bertalanffy. Here, we present a physiologically structured individual growth model, which incorporates an energy budget and optimizes energy allocation to growth, reproduction, and reserves. Growth, maturation, and reproductive output emerge as a result of life-history optimization to specific physiological rates and mortality conditions. To assess which processes can lead to temperature-size rule-type life histories, we simulate 42 scenarios that differ in temperature and body size dependencies of intake, metabolism, and mortality rates. Results show that the temperature-size rule can emerge in two ways. The first way requires both intake and metabolism to increase with temperature, but the temperature-body size interaction of the two rates must lead to relatively faster intake increase in small individuals and relatively larger metabolism increase in large ones. The second way requires only higher temperature-driven natural mortality and faster intake rates in early life (no change in metabolic rates is needed). This selects for faster life histories with earlier maturation and increased reproductive output. Our model provides a novel mechanistic and evolutionary framework for identifying the conditions necessary for the temperature-size rule. It shows that the temperature-size rule is likely to reflect both physiological changes and life-history optimization and that use of von Bertalanffy-type models, which do not include reproduction processes, can hinder our ability to understand and predict ectotherm responses to climate change.

AbstractDetermining the resilience of a species or population to climate change stressors is an important but difficult task because resilience can be affected both by genetically based variation and by various types of phenotypic plasticity. In addition, most of what is known about organismal responses is for single stressors in isolation, but environmental change involves multiple environmental factors acting in combination. Here, our goal is to summarize what is known about phenotypic plasticity in fishes in response to high temperature and low oxygen (hypoxia) in combination across multiple timescales, to ask how much resilience plasticity may provide in the face of climate change. There are relatively few studies investigating plasticity in response to these environmental stressors in combination; but the available data suggest that although fish have some capacity to adjust their phenotype and compensate for the negative effects of acute exposure to high temperature and hypoxia through acclimation or developmental plasticity, compensation is generally only partial. There is very little known about intergenerational and transgenerational effects, although studies on each stressor in isolation suggest that both positive and negative impacts may occur. Overall, the capacity for phenotypic plasticity in response to these two stressors is highly variable among species and extremely dependent on the specific context of the experiment, including the extent and timing of stressor exposure. This variability in the nature and extent of plasticity suggests that existing phenotypic plasticity is unlikely to adequately buffer fishes against the combined stressors of high temperature and hypoxia as our climate warms.

AbstractPredictions for climate change-to lesser and greater extents-reveal a common scenario in which marine waters are characterized by a deadly trio of stressors: higher temperatures, lower oxygen levels, and acidification. Ectothermic taxa that inhabit coastal waters, such as shellfish, are vulnerable to rapid and prolonged environmental disturbances, such as heatwaves, pollution-induced eutrophication, and dysoxia. Oxygen transport capacity of the hemolymph (blood equivalent) is considered the proximal driver of thermotolerance and respiration in many invertebrates. Moreover, maintaining homeostasis under environmental duress is inextricably linked to the activities of the hemolymph-based oxygen transport or binding proteins. Several protein groups fulfill this role in marine invertebrates: copper-based extracellular hemocyanins, iron-based intracellular hemoglobins and hemerythrins, and giant extracellular hemoglobins. In this brief text, we revisit the distribution and multifunctional properties of oxygen transport proteins, notably hemocyanins, in the context of climate change, and the consequent physiological reprogramming of marine invertebrates.

AbstractThere is a scientific debate whether oxygen concentration may be a factor driving the pattern of size decrease at higher temperature. Central to this debate is the fact that oxygen availability relative to demand for living organisms decreases with increasing temperature. We examined whether rotifers Lecane inermis exposed to hypoxic conditions would evolve smaller sizes than rotifers exposed to normoxic conditions, using experimental evolution with the same fluctuating temperature but differentiated by three regimes of oxygen availability: normoxia, hypoxia throughout the whole thermal range, and hypoxia only at the highest temperature. Immediately after the six-month experiment (more than 90 generations), we tested the plasticity of size responses to temperature in three post-evolution groups, and we related these responses to fitness. The results show that normoxic rotifers had evolved significantly larger sizes than two hypoxic rotifer groups, which were similar in size. All three groups displayed similar plastic body size reductions in response to warming over the range of temperatures they were exposed to during the period of experimental evolution, but they showed different and complex responses at two temperatures below this range. Any type of plastic response to different temperatures resulted in a similar fitness pattern across post-evolution groups. We conclude that (i) these rotifers showed a genetic basis for the pattern of size decrease following evolution under both temperature-dependent and temperature-independent hypoxia; and (ii) plastic body size responds consistently to temperatures that are within the thermal range that the rotifers experienced during their evolutionary history, but responses become more noisy at novel temperatures, suggesting the importance of evolutionary responses to reliable environmental cues.

AbstractGlobal ocean O₂ content has varied significantly across the eons, both shaping and being shaped by the evolutionary history of life on planet Earth. Indeed, past O₂ fluctuations have been associated with major extinctions and the reorganization of marine biota. Moreover, its most recent iteration-now anthropogenically driven-represents one of the most prominent challenges for both marine ecosystems and human societies, with ocean deoxygenation being regarded as one of the main drivers of global biodiversity loss. Yet ocean deoxygenation has received far less attention than concurrent environmental variables of marine climate change, namely, ocean warming and acidification, particularly in the field of experimental marine ecology. Together with the lack of consistent criteria defining gradual and acute changes in O₂ content, a general lack of multifactorial studies featuring all three drivers and their interactions prevents an adequate interpretation of the potential effects of extreme and gradual deoxygenation. We present a comprehensive overview of the interplay between O₂ and marine life across space and time and discuss the current knowledge gaps and future steps for deoxygenation research. This work may also contribute to the ongoing call for an integrative perspective on the combined effects of these three drivers of change for marine organisms and ecosystems worldwide.

AbstractThe frequency, magnitude, and duration of marine heatwaves and deoxygenation events are increasing globally. Recent research suggests that their co-occurrence is more common than previously thought and that their combination can have rapid, dire biological impacts. We used the sea urchin Echinometra lucunter to determine whether mortality occurs faster when deoxygenation events are combined with extreme heating (compound events), compared to deoxygenation events alone. We also tested whether prior exposure to local heatwave conditions accentuates the impacts of compound events. Animals were first exposed for five days to either ambient temperature (28 °C) or a warmer temperature that met the minimum criteria for a local heatwave (30.5 °C). Animals were then exposed to hypoxia, defined as oxygen levels 35% below their average critical oxygen limit, combined with ambient or extreme field temperatures (28 °C, 32 °C). Subsets of animals were removed from the hypoxic treatments every 3 hours for 24 hours to determine how long they could survive. Prior exposure to heatwave conditions did not help or hinder survival under hypoxic conditions, and animals exposed to hypoxia under ambient temperatures experienced little mortality. However, when hypoxia was coupled with extreme temperatures (32 °C), 55% of the animals died within 24 hours. On the reefs at our Panama study site, we found that extreme hypoxic conditions only ever occurred during marine heatwave events, with four compound events occurring in 2018. These results show that short durations (∼1 day) of compound events can be catastrophic and that increases in their duration will severely threaten sea urchin populations.

AbstractPeriodic episodes of low oxygen (hypoxia) and elevated CO₂ (hypercapnia) accompanied by low pH occur naturally in estuarine environments. Under the influence of climate change, the geographic range and intensity of hypoxia and hypercapnic hypoxia are predicted to increase, potentially jeopardizing the survival of economically and ecologically important organisms that use estuaries as habitat and nursery grounds. In this review we synthesize data from published studies that evaluate the impact of hypoxia and hypercapnic hypoxia on the ability of crustaceans and bivalve molluscs to defend themselves against potential microbial pathogens. Available data indicate that hypoxia generally has suppressive effects on host immunity against bacterial pathogens as measured by in vitro and in vivo assays. Few studies have documented the effects of hypercapnic hypoxia on crustaceans or bivalve immune defense, with a range of outcomes suggesting that added CO₂ might have additive, negative, or no interactions with the effects of hypoxia alone. This synthesis points to the need for more partial pressure of O₂ × low pH factorial design experiments and recommends the development of new host∶pathogen challenge models incorporating natural transmission of a wide range of viruses, bacteria, and parasites, along with novel in vivo tracking systems that better quantify how pathogens interact with their hosts in real time under laboratory and field conditions.

AbstractThe copepods of coastal seas are experiencing warming water temperatures, which increase their oxygen demand. In addition, many coastal seas are also losing oxygen because of deoxygenation due to cultural eutrophication. Warming coastal seas have changed copepod species' composition and biogeographic boundaries and, in many cases, resulted in copepod communities that have shifted in size distribution to smaller species. While increases in ambient water temperatures can explain some of these changes, deoxygenation has also been shown to result in reduced copepod growth rates, reduced size at adulthood, and altered species composition. In this review we focus on the interactive effects of temperature and dissolved oxygen on pelagic copepods, which dominate coastal zooplankton communities. The uniformity in ellipsoidal shape, the lack of external oxygen uptake organs, and the pathway of oxygen uptake through the copepod's integument make calanoid copepods ideal candidates for testing the use of an allometric approach to predict copepod size with increasing water temperatures and decreasing oxygen in coastal seas. Considering oxygen and temperature as a combined and interactive driver in coastal ecosystems will provide a unifying approach for future predictions of coastal copepod communities and their impact on fisheries and biogeochemical cycles. Given the prospect of increased oxygen limitation of copepods in warming seas, increased knowledge of the physiological ecology of present-day copepods in coastal deoxygenated zones can provide insights into the copepod communities that will inhabit a future warmer ocean.