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New codend designs to improve the size selectivity of fyke net for narrow-clawed crayfish (Pontastacus leptodactylus) 提高窄爪螯虾网尺寸选择性的新编码设计
IF 1.5 4区 农林科学 Q3 FISHERIES Pub Date : 2022-03-08 DOI: 10.1051/alr/2022014
Verena Trenkel (Handling Editor), Mehmet Cilbiz , Celalettin Aydın , Bent Herrmann
The narrow-clawed crayfish (Pontastacus leptodactylus) is one of the most ecologically and economically important freshwater species in Turkey. However, the harvest of this species has declined from 7937 t in 1984 to 696 t in 2019. One of primary reasons for this decrease in stock is the use of fishing gears with poor size selectivity. In this study, new codend designs were investigated to improve the size selectivity of fyke nets for P. leptodactylus. Seven codends of three different designs were tested: (1) a commercially used standard codend with 34 mm mesh size (Com34); (2) sorting grids with 20 (SG20), 25 (SG25) and 30 mm (SG30) bar spacing attached to the last circle of the net; and (3) a stiff rigged net, the last part of the fyke nets (codend) with 34 (SRN34), 42 (SRN42) and 50 mm (SRN50) mesh size. The average length at 50% retention probability (L50) and selection range (SR) (L75L25) values of Com34 were 9.4 cm and 3.1 cm, respectively. For the SG20, SG25 and SG30 grids, the L50 values were 11.3 cm, 11.6 cm and 12.0 cm, while the SR values were 1.4 cm, 1.3 cm and 0.6 cm, respectively. For the SRN34, SRN42 and SRN50 codends, the L50 values were 10.9 cm, 11.6 cm and 11.6 cm, while the SR values were 2.1 cm, 2.1cm and 1.1 cm, respectively. Overall, the commercial codend resulted in lower L50 values when the minimum conservation reference length of 10 cm was considered. However, all tested new codend designs showed improved selectivity compared with the standard, with optimum results obtained with SG20. SG20 grid decreased discarding by 15.7% compared to the classic commercial fyke net; thus, this is a very important result for the sustainability of natural P. leptodactylus stocks.
窄爪小龙虾(Pontastacus leptodactylus)是土耳其最重要的生态和经济淡水物种之一。然而,该物种的收获量从1984年的7937吨下降到2019年的696吨。鱼类数量减少的主要原因之一是使用了尺寸选择性差的渔具。本研究探讨了新的码尾设计,以提高对细趾假单足虫网具的大小选择性。测试了三种不同设计的七种码头:(1)商用标准码头,网目尺寸为34 mm (Com34);(2)在网的最后一圈附着20 (SG20)、25 (SG25)和30 mm (SG30)棒距的分选网格;(3)硬式吊网,最后一部分的网目尺寸分别为34 (SRN34)、42 (SRN42)和50 mm (SRN50)。Com34在50%保留概率下的平均长度(L50)和选择范围(SR) (l75 ~ l25)分别为9.4 cm和3.1 cm。对于SG20、SG25和SG30栅格,L50值分别为11.3、11.6和12.0 cm, SR值分别为1.4、1.3和0.6 cm。SRN34、SRN42和SRN50的L50值分别为10.9 cm、11.6 cm和11.6 cm, SR值分别为2.1cm、2.1cm和1.1 cm。总体而言,当考虑最小保护参考长度为10 cm时,商业规范导致L50值较低。然而,与标准相比,所有测试的新编码端设计都显示出更高的选择性,其中SG20获得了最佳结果。与传统的商业电网相比,SG20电网的丢弃量减少了15.7%;因此,这是一个非常重要的结果,对自然的纤毛霉种群的可持续性。
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引用次数: 0
Metazoan parasite community as a potential biological indicator in juveniles of the starry smooth-hound Mustelus asterias Cloquet, 1819 (Carcharhiniformes Triakidae) 星滑犬幼犬后生动物寄生群落的潜在生物学指示
IF 1.5 4区 农林科学 Q3 FISHERIES Pub Date : 2022-03-08 DOI: 10.1051/alr/2022002
Claudia Gérard , Maxime R. Hervé , Héloïse Hamel , Mélanie Gay , Michel Barbier , Thomas Barreau , Ryan Carnegie
The starry smooth-hound Mustelus asterias is a near-threatened coastal shark in Europe, whose parasitofauna is largely unknown. We studied metazoan parasites of 20 immature sharks (13 males and seven females) from the English Channel and we examined their relationships with host condition and their use as host bioindicators. All the sharks were parasitized by one to six metazoan taxa among the twelve recorded in the whole sampling (one trematode, six cestodes and two nematodes trophically-transmitted; one monogenean, one copepod and one myxosporean on gills), with a mean abundance of 30.5 ± 21.4 parasites per fish (myxosporeans not included). The three major taxa were in decreasing order: the nematode Acanthocheilus rotundatus (prevalence: 75%, Confidence Interval 53–89%), the cestode Eutetrarhynchus sp. (70%, CI 48–85%), and the monogenean Erpocotyle laevis (60%, CI 39–78%). The gill copepod Kroyeria lineata and the gut nematode Proleptus obtusus were identified as significant pathogens. Parasite community differed between males and females despite their immature stage, suggesting early spatial sex-segregation, with E. laevis, Eutetrarhynchus sp. and Anthobothrium sp. proposed as tags. We discuss results in terms of host fitness loss and information given by parasites on diet ecology and stock discrimination. We recommend incorporating parasitology in further research to improve shark conservation and management.
星滑猎犬Mustelus asterias是一种濒临灭绝的欧洲沿海鲨鱼,其寄生动物在很大程度上是未知的。我们研究了来自英吉利海峡的20只未成熟鲨鱼(13只雄性和7只雌性)的后生动物寄生虫,并研究了它们与宿主状况的关系以及它们作为宿主生物指标的用途。所有鲨鱼均寄生于12个后生动物类群中的1 ~ 6个(吸虫1个、囊虫6个、线虫2个);1条单系、1条桡足类和1条粘孢子虫(鳃上),平均每条鱼的寄生虫丰度为30.5±21.4(不包括粘孢子虫)。3个主要分类群依次为圆形棘虫(Acanthocheilus rotundatus)(患病率75%,置信区间53 ~ 89%)、全四鼻虫(70%,置信区间48 ~ 85%)和单系足尾虫(60%,置信区间39 ~ 78%)。鳃类桡足动物克罗伊亚(Kroyeria lineata)和肠道线虫钝尾拟龙(Proleptus obtusus)被确定为重要病原体。在未成熟阶段,雌雄寄生蜂的群落结构就存在差异,提示存在较早的空间性别分离,以E. laevis、Eutetrarhynchus sp.和Anthobothrium sp.为标记。我们讨论了宿主适合度损失的结果和寄生虫提供的关于饲料生态和种群识别的信息。我们建议将寄生虫学纳入进一步的研究,以改善鲨鱼的保护和管理。
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引用次数: 0
Interacting climate change effects on mussels (Mytilus edulis and M. galloprovincialis) and oysters (Crassostrea gigas and Ostrea edulis): experiments for bivalve individual growth models 气候变化对贻贝(Mytilus edulis和M. galloprovincialis)和牡蛎(Crassostrea gigas和Ostrea edulis)的相互影响:双壳类个体生长模型实验
IF 1.5 4区 农林科学 Q3 FISHERIES Pub Date : 2022-03-08 DOI: 10.1051/alr/2022001
Pauline Kamermans , Camille Saurel , Pierre Boudry
The physiological response of two species of mussels (Mytilus edulis and M. galloprovincialis) and two species of oysters (Crassostrea gigas and Ostrea edulis) to temperature, oxygen levels and food concentration, factors likely to vary as a result of climate change, was determined experimentally. Bivalves of similar size from different origins were exposed to six temperatures (3, 8, 15, 20, 25 and 30 °C) at two food regimes (2 and 10 μg Chl a L−1) for 6 weeks. In a parallel running experiment M. edulis from the same batches were exposed to three different temperatures (15, 20 and 25 °C) and three different oxygen levels (30, 50 and 100%) at two food regimes (2 and >8 μg Chl a L−1) for 3–4 weeks. Survival during the experiment ranged from 93% to 100% except for the mussels exposed to 30 °C which showed 100% mortality after three to 32 days. Higher food conditions showed higher optimal temperatures for growth of mussels and oysters. In addition, at the high food treatment, reduced O2 saturation resulted in lower growth of mussels. At the low food treatment there were no differences in growth among the different O2 levels at the same temperature. At high food concentration treatment, M. edulis growth was higher with low temperature and high oxygen level. Condition index was higher at higher food concentrations and decreased with increasing temperature. In addition, condition was lower at low oxygen saturation. Lower clearance rates were observed at high food concentrations. At 100% saturation of oxygen, mussel clearance rate increased with temperature at High food regime, but not at Low food regime. Mussel clearance rates were significantly reduced with low oxygen concentrations together with high temperature. Oxygen consumption significantly increased with temperature. Oxygen saturation was the main factor affecting mussel clearance rate. High temperature and low oxygen concentration combined significantly reduced clearance rate and increased oxygen consumption. These response curves can be used to improve parameterisation of individual shellfish growth models taking into consideration factors in the context of climate change: temperature, food concentration, oxygen concentration and their interactions. The observation that abiotic factors interact in affecting mussels and oysters is an important result to take into account.
实验研究了两种贻贝(Mytilus edulis和M. galloprovincialis)和两种牡蛎(Crassostrea gigas和Ostrea edulis)对温度、氧气水平和食物浓度等可能因气候变化而变化的因素的生理反应。研究人员将来自不同来源的大小相似的双壳贝在6个温度(3、8、15、20、25和30°C)和两种食物体系(2和10 μg Chl a L−1)下暴露6周。在平行运行的实验中,同一批次的毛竹芽孢杆菌在两种食物体系(2和8 μg Chl a L−1)下暴露于三种不同的温度(15、20和25°C)和三种不同的氧气水平(30、50和100%),持续3-4周。实验期间的存活率为93%至100%,但暴露于30°C的贻贝在3至32天后死亡率为100%。较高的食物条件表明贻贝和牡蛎生长的最佳温度较高。此外,在高食物处理下,氧饱和度降低导致贻贝生长减慢。在低食处理下,相同温度下不同氧含量的植株生长无差异。在高食物浓度处理下,毛竹芽孢杆菌在低温和高氧条件下生长更快。条件指数随食物浓度的升高而升高,随温度的升高而降低。此外,低氧饱和度条件较低。在高浓度食物中观察到较低的清除率。在100%氧饱和度下,高摄食条件下贻贝清除率随温度升高而升高,而低摄食条件下则不升高。低氧和高温条件下贻贝清除率显著降低。耗氧量随温度的升高而显著增加。氧饱和度是影响贻贝清除率的主要因素。高温和低氧浓度的结合显著降低了清除率,增加了耗氧量。这些响应曲线可用于改善单个贝类生长模型的参数化,考虑气候变化背景下的因素:温度、食物浓度、氧气浓度及其相互作用。观察到非生物因素在影响贻贝和牡蛎时相互作用,这是一个值得考虑的重要结果。
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引用次数: 0
Molecular identification, life cycle characterization, and hatchery seed production of dwarf oysters from the Ebro Delta (Spain) 西班牙埃布罗三角洲矮生牡蛎的分子鉴定、生命周期特征及孵化种生产
IF 1.5 4区 农林科学 Q3 FISHERIES Pub Date : 2022-03-08 DOI: 10.1051/alr/2022005
Patricia Prado , Margarita Fernández , David Cordero , Carlos Saavedra , Francesca Carella , Carles Alcaraz , Ignasi Gairin , Pauline Kamermans
Dwarf oysters in the Ebro Delta are only observed growing on local pen shells, Pinna nobilis L., currently under extinction risk. The species identification of these populations is uncertain, given recent genetic classifications of dwarf oysters of Ostrea stentina species complex. Hence, the first objective of this study was to confirm the identity of dwarf oysters associated to P. nobilis in the Ebro Delta. Then, we aimed to assess the viability of hatchery and nursery production of O. stentina for potential conservation programs using diets based on a mix of live microalgae species or the commercial Shellfish Diet 1800®. Our results showed that COI sequences obtained were specific to O. stentina. Significant differences in the growth and survival of larvae between diet treatments were observed in 3 days. For the live diet, the larval period lasted from 15 to 22 days (299.2 ± 4 µm) whereas it took up to 36 days using the Shellfish Diet 1800® (280 ± 7.2 µm), with an overall mortality of 72.3 vs. 99.3%, respectively for each diet. Besides, no seed survival was observed for the commercial diet after a 10-day-fixation attempt. In contrast, ca. 16% of the individuals on the live diet that were allowed to settle reached the juvenile stage and could be released to the environment. Histological examination of obtained seed did not present any sign of disease and showed gonadal development for both sexes at ca. 6 months of age (16.1–19.1 mm). The growth curve obtained was fitted to a Schnute-Richards growth model which returned ages of up to 10 years for maximum local sizes of 45 mm. We conclude that although the Shellfish Diet 1800® is not an adequate diet for O. stentina, its hatchery production is feasible and might provide support to future conservation actions.
在埃布罗三角洲,人们只观察到在当地的笔壳上生长的矮牡蛎,Pinna nobilis L.,目前正面临灭绝的危险。鉴于Ostrea stentina种复合体的矮牡蛎最近的遗传分类,这些种群的物种鉴定是不确定的。因此,本研究的第一个目的是确认在埃布罗三角洲与P. nobilis相关的矮牡蛎的身份。然后,我们的目标是评估O. stentina的孵化场和苗圃生产的可行性,以潜在的保护计划为基础,使用基于活微藻物种的混合饲料或商业贝类饲料1800®。结果表明,所获得的COI序列对大叶豆具有特异性。3 d后,不同日粮处理的幼虫生长和存活率均有显著差异。活体饲料的幼虫期为15 ~ 22 d(299.2±4µm),而贝类饲料1800®(280±7.2µm)的幼虫期为36 d,两种饲料的总死亡率分别为72.3和99.3%。此外,在固定10天后,未观察到商业饲料的种子存活。相比之下,被允许定居的活食个体中约有16%达到了幼鱼阶段,可以被释放到环境中。对获得的种子进行组织学检查未发现任何疾病迹象,并在大约6个月大(16.1-19.1 mm)时显示两性性腺发育。所得的生长曲线拟合到Schnute-Richards生长模型中,该模型对最大局部尺寸为45 mm的情况返回了长达10年的年龄。我们得出结论,尽管贝类饲料1800®不适合大纹贝,但其孵化场生产是可行的,并可能为未来的保护行动提供支持。
{"title":"Molecular identification, life cycle characterization, and hatchery seed production of dwarf oysters from the Ebro Delta (Spain)","authors":"Patricia Prado ,&nbsp;Margarita Fernández ,&nbsp;David Cordero ,&nbsp;Carlos Saavedra ,&nbsp;Francesca Carella ,&nbsp;Carles Alcaraz ,&nbsp;Ignasi Gairin ,&nbsp;Pauline Kamermans","doi":"10.1051/alr/2022005","DOIUrl":"10.1051/alr/2022005","url":null,"abstract":"<div><div>Dwarf oysters in the Ebro Delta are only observed growing on local pen shells, <em>Pinna nobilis</em> L., currently under extinction risk. The species identification of these populations is uncertain, given recent genetic classifications of dwarf oysters of <em>Ostrea stentina</em> species complex. Hence, the first objective of this study was to confirm the identity of dwarf oysters associated to <em>P. nobilis</em> in the Ebro Delta. Then, we aimed to assess the viability of hatchery and nursery production of <em>O. stentina</em> for potential conservation programs using diets based on a mix of live microalgae species or the commercial Shellfish Diet 1800<sup>®</sup>. Our results showed that COI sequences obtained were specific to <em>O. stentina</em>. Significant differences in the growth and survival of larvae between diet treatments were observed in 3 days. For the live diet, the larval period lasted from 15 to 22 days (299.2 ± 4 µm) whereas it took up to 36 days using the Shellfish Diet 1800<sup>®</sup> (280 ± 7.2 µm), with an overall mortality of 72.3 vs. 99.3%, respectively for each diet. Besides, no seed survival was observed for the commercial diet after a 10-day-fixation attempt. In contrast, ca. 16% of the individuals on the live diet that were allowed to settle reached the juvenile stage and could be released to the environment. Histological examination of obtained seed did not present any sign of disease and showed gonadal development for both sexes at ca. 6 months of age (16.1–19.1 mm). The growth curve obtained was fitted to a Schnute-Richards growth model which returned ages of up to 10 years for maximum local sizes of 45 mm. We conclude that although the Shellfish Diet 1800<sup>®</sup> is not an adequate diet for <em>O. stentina</em>, its hatchery production is feasible and might provide support to future conservation actions.</div></div>","PeriodicalId":55491,"journal":{"name":"Aquatic Living Resources","volume":"35 ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143331936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lactic acid bacteria as probiotics in sustainable development of aquaculture 乳酸菌作为益生菌在水产养殖可持续发展中的作用
IF 1.5 4区 农林科学 Q3 FISHERIES Pub Date : 2022-03-08 DOI: 10.1051/alr/2022011
Antonio Figueras, Anna Chizhayeva , Alma Amangeldi , Yelena Oleinikova , Aigul Alybaeva , Amankeldy Sadanov
Industrial aquaculture is a dynamic area capable of solving problems of healthy nutrition and food security. Increase of organic pollution, number of opportunistic microorganisms in the aquatic environment of fish farms and the global contamination of feed by mycotoxigenic fungi are serious problems of industrial fish cultivation. The results are weakening of the general condition of fish, immunosuppression, the occurrence of various diseases complicated by drug resistance, the accumulation of antibiotics and chemical compounds in tissues. Probiotics can be an alternative to antibiotics. The use of probiotics is also one of the biological methods for maintaining and restoring the normal physiological state of fish and increasing their productivity. The aim of this review is the scientific justification of the use of lactic acid bacteria as the safest microorganisms in the development of probiotics for aquaculture. The review presented provides criteria for selecting candidate strains for effective probiotics development. The advantages of lactic acid bacteria for the prevention or control of infectious diseases in cultured fish are considered. Lactic acid bacteria are representatives of the fish microbiota, they have antagonistic activity against opportunistic pathogens, fungi and viruses that cause microbiological spoilage of feed, pollute water bodies, and cause diseases of aquatic animals. The review provides information on various researches in which lactic acid bacteria or products derived from them have been used to assess their potential in aquaculture. Numerous scientific studies prove the value of this vast group of microorganisms for the prevention and treatment of fish diseases, for increasing the resistance of aquatic animals to infectious diseases and various stresses, for improving their survival and productivity, and for improving water sanitation in fish reservoirs. Increased use of effective probiotic lactic acid bacteria in aquaculture can make the fish sector safer, more productive and friendly to the environment and human well-being, and will contribute to the sustainable development of aquaculture.
工业水产养殖是一个充满活力的领域,能够解决健康营养和粮食安全问题。有机污染的增加、养鱼场水生环境中机会微生物数量的增加以及全球范围内产毒真菌对饲料的污染是工业养鱼面临的严重问题。结果是鱼的一般状况减弱,免疫抑制,发生各种疾病并伴有耐药性,抗生素和化合物在组织中积累。益生菌可以替代抗生素。益生菌的使用也是维持和恢复鱼类正常生理状态,提高其生产力的生物学方法之一。这篇综述的目的是科学地证明在水产养殖益生菌的开发中使用乳酸菌作为最安全的微生物。本文的综述为开发有效益生菌的候选菌株提供了标准。讨论了乳酸菌在养殖鱼类传染病防治中的优势。乳酸菌是鱼类微生物群的代表,对引起饲料微生物变质、水体污染和水生动物疾病的条件致病菌、真菌和病毒具有拮抗活性。本综述提供了利用乳酸菌或其衍生产品评估其在水产养殖中的潜力的各种研究的信息。大量的科学研究证明了这一大群微生物在预防和治疗鱼类疾病、提高水生动物对传染病和各种压力的抵抗力、提高它们的存活率和生产力以及改善养鱼池的水卫生方面的价值。在水产养殖中增加使用有效的益生菌乳酸菌可以使鱼类部门更安全、更高产、对环境和人类福祉更友好,并将有助于水产养殖的可持续发展。
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引用次数: 0
Age, growth and maturity of frigate tuna (Auxis thazard Lacepède, 1800) in the Southeastern Brazilian coast 巴西东南海岸护卫舰金枪鱼的年龄、生长和成熟(Auxis thazard lacep<e:1>, 1800)
IF 1.5 4区 农林科学 Q3 FISHERIES Pub Date : 2022-03-08 DOI: 10.1051/alr/2022010
Flavia Lucena Fredou, Juliana M.S. Vieira , Paulo A.S. Costa , Adriana C. Braga , Ricardo R.B. São-Clemente , Carlos E.L. Ferreira , Jodir P. Silva
Age, growth and maturity of frigate tuna (Auxis thazard) were studied for the first time in Southeast Brazil. A total of 650 fish (265–494 mm Fork Length) were sampled from beach-seine landings between March 2018 and February 2019. Sectioned fin spines from 548 specimens were processed and age was determined by counting and measuring of increments. Growth parameters were fitted to length-at-age data using the von Bertalanffy growth function and were compared to the literature using the growth performance index (Phi). Macro and microscopic characterization of gonads and physiological indexes were used for the analysis of the reproductive cycle. The length distribution did not differ by sex. Assigned ages were between 0+ and 4 years, with annual increments formed during winter. Coefficient of variation between readings was 11%. Growth differed by sex, and the von Bertalanffy growth parameters were L = 471 mm; k = 0.47 year−1; and t0 = –1.46 year−1 for females; L = 498 mm; k = 0.35 year−1; and t0 = –2.01 year−1 for males. Phi was 5.0 for both sexes. Estimated growth rate is among the lowest recorded for the species, possibly related to the method used for age and growth assessment. Gonadosomatic index peaked in December, associated with the upwelling of the South Atlantic Central Waters, at a temperature of approximately 16 °C. Spawning activity in colder waters has never been reported for this species and may be influenced by a richer habitat caused by the intrusion of more nutritive rich waters during spring-summer, which allows larval development. Length-at-first-maturity (L50) was 345.4 mm for females and 329.8 mm for males.
在巴西东南部首次对护卫舰金枪鱼(Auxis thazard)的年龄、生长和成熟度进行了研究。在2018年3月至2019年2月期间,共从海滩围网上岸的650条鱼(叉长265-494毫米)取样。对548个标本的鳍刺切片进行了处理,并通过计数和测量增量来确定年龄。使用von Bertalanffy生长函数拟合生长参数与年龄长度数据,并使用生长性能指数(Phi)与文献进行比较。利用性腺的宏观和微观特征及生理指标分析其生殖周期。长度分布没有性别差异。分配年龄在0 ~ 4岁之间,在冬季形成年度增量。读数之间的变异系数为11%。生长存在性别差异,von Bertalanffy生长参数为L∞= 471 mm;K = 0.47 year−1;女性t0 = -1.46 year - 1;L∞= 498 mm;K = 0.35 year−1;男性t0 = -2.01 year - 1。男女的Phi值都是5.0。估计的生长率是该物种的最低记录之一,可能与用于年龄和生长评估的方法有关。促性腺指数在12月达到峰值,与南大西洋中部水域上升流有关,温度约为16°C。该物种在较冷水域的产卵活动从未报道过,可能受到春夏期间营养丰富的水域入侵造成的更丰富栖息地的影响,这有利于幼虫的发育。初熟体长(L50)雌性为345.4 mm,雄性为329.8 mm。
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引用次数: 0
Correction Notice: Age, growth and maturity of frigate tuna (Auxis thazard Lacepède, 1800) in the Southeastern Brazilian coast 更正通知:巴西东南海岸护卫舰金枪鱼(Auxis thazard lacep<e:1>, 1800)的年龄、生长和成熟度
IF 1.5 4区 农林科学 Q3 FISHERIES Pub Date : 2022-01-01
Juliana M.S. Vieira , Paulo A.S. Costa , Adriana C. Braga , Ricardo R.B. São-Clemente , Carlos E.L. Ferreira , Jodir P. Silva
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引用次数: 0
Direct multiplex PCR-NALFIA to inform marine conservation: Use of an innovative diagnostic tool for the detection of Ostrea edulis larvae 直接多重PCR-NALFIA为海洋保护提供信息:使用一种创新的诊断工具来检测毛竹Ostrea edulis幼虫
IF 1.5 4区 农林科学 Q3 FISHERIES Pub Date : 2021-04-29 DOI: 10.1051/alr/2021023
Andreas Benkens , Claudia Buchholz , Bernadette Pogoda , Carsten Georg Harms , Carlos Saavedra
The European oyster Ostrea edulis played a key role in the North Sea by providing several ecosystem functions and services. Today, O. edulis is classified as severely degraded or functionally extinct in Europe. Marine conservation is focusing on biogenic reef restoration, namely the restoration of O. edulis in Natura 2000 sites of the North Sea. The identification of oyster larvae related to natural spatfalls of restored reefs and monitoring of larval drift is a key aspect of marine protected area management. Morphological identification and distinction from other abundant bivalve larvae using microscopy is difficult. Existing molecular biological methods are expensive and bound to stationary laboratory equipment, or are inadequate in the visualization. In this study, we identified nucleic acid lateral flow immunoassay (NALFIA), a well-established tool in human pathogen diagnostics, as an efficient approach for point-of-care (POC) testing in marine monitoring. Based on the genetic sequence of the mitochondrial cytochrome b of O. edulis, forward and reverse primers were developed. The reverse primer was labelled with fluorescent dye FITC, forward primer with biotin. Reaction on the lateral flow stripe could be realized with a single O. edulis larva in direct PCR with multiplex primers in a portable PCR-cycler. The established NALFIA system can distinguish O. edulis larvae from Crassostrea gigas and Mytilus edulis larvae, respectively. This method offers new approaches in POC testing in marine research and monitoring. It gives quick and clear results, is inexpensive, and could be easily adapted to other species of interest.
欧洲牡蛎(Ostrea edulis)通过提供多种生态系统功能和服务,在北海发挥了关键作用。今天,在欧洲,毛竹被归类为严重退化或功能性灭绝。海洋保育的重点是生物礁的恢复,即在北海的Natura 2000地点恢复O. edulis。与恢复珊瑚礁自然落落有关的牡蛎幼体鉴定和幼体漂移监测是海洋保护区管理的一个重要方面。形态学鉴定和从其他丰富的双壳类幼虫用显微镜区分是困难的。现有的分子生物学方法是昂贵的,并且受限于固定的实验室设备,或者在可视化方面是不够的。在这项研究中,我们确定了核酸侧流免疫分析法(NALFIA),一种完善的人类病原体诊断工具,作为海洋监测中即时检测(POC)的有效方法。根据毛竹线粒体细胞色素b的基因序列,开发了正、反向引物。反向引物用荧光染料FITC标记,正向引物用生物素标记。在便携式PCR仪上,用多引物直接PCR,可以实现单株毛豆幼虫在横向流动条带上的反应。所建立的NALFIA系统可以分别将O. edulis幼虫与长牡蛎(creasstrea gigas)和Mytilus edulis幼虫区分开来。该方法为海洋研究和监测中的POC测试提供了新的途径。它给出快速和清晰的结果,价格低廉,并且可以很容易地适应其他感兴趣的物种。
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引用次数: 0
Histology of Tritia mutabilis gonads: using reproductive biology to support sustainable fishery management 变异小偃麦草性腺组织学:利用生殖生物学支持可持续渔业管理
IF 1.5 4区 农林科学 Q3 FISHERIES Pub Date : 2021-04-29 DOI: 10.1051/alr/2021006
Alicia Mallet , Jean-Yves Jouvenel , Morgane Broyon , Nelly Pirot , Benjamin Geffroy , Ellen Kenchington
The mutable nassa, Tritia mutabilis, a marine gastropod that is widely exploited on the Adriatic coast is an important source of income for small-scale fishermen in the Mediterranean Sea, particularly in the Gulf of Lion. However, the lack of knowledge on the ecology and biology of this species limits our capacities to propose and produce an effective management plan. As a result, stocks are currently declining, especially in Italy. In order to optimize a management plan for this fishery, we designed a study to better characterize the reproductive biology of T. mutabilis, using gonad histology and performing a regular monitoring of population size frequency. The average shell height of individuals during the breeding period was 24 ± 2.7 mm for males and 30 ± 3.7 mm for females. The presence of small females (10 mm) and large males (32 mm) in the whole sample challenged previous assumptions regarding protandry (sex change from male to female). The size at first maturity was estimated for males and females at 17.5 mm and 24.4 mm shell height, respectively. In Italy, current management measures include a minimum conservation reference size of 20 mm in shell height. Therefore, it is likely that many individuals that did not reproduce are being caught, which could partly explain the decline observed, despite conservation measures introduced more than ten years ago. Overall, our study provides some baseline information to establish, in consultation with fishermen, management measures for this small-scale fishery in France.
在亚得里亚海沿岸被广泛开发的一种海洋腹足类动物,是地中海,特别是狮子湾小规模渔民的重要收入来源。然而,由于对这一物种的生态学和生物学知识的缺乏,限制了我们提出和制定有效管理计划的能力。因此,库存目前正在下降,尤其是在意大利。为了优化该渔业的管理计划,我们设计了一项研究,利用性腺组织学和对种群大小频率进行定期监测,以更好地表征突变T.的生殖生物学。繁殖期个体平均壳高雄性为24±2.7 mm,雌性为30±3.7 mm。在整个样本中,小雌性(10毫米)和大雄性(32毫米)的存在挑战了之前关于原雄制(从雄性到雌性的性别变化)的假设。雄性和雌性初成熟时的壳高分别为17.5 mm和24.4 mm。在意大利,目前的管理措施包括贝壳高度的最小保护参考尺寸为20毫米。因此,很可能许多没有繁殖的个体被捕获,这可以部分解释观察到的下降,尽管十多年前引入了保护措施。总的来说,我们的研究提供了一些基线信息,在与渔民协商的情况下,为法国这种小规模渔业制定管理措施。
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引用次数: 0
Do efficient small-scale fishers stay active in eras of introducing individual transferable quotas? Evidence from Denmark 在引入个人可转让配额的时代,高效的小规模渔民还能保持活跃吗?来自丹麦的证据
IF 1.5 4区 农林科学 Q3 FISHERIES Pub Date : 2021-04-29 DOI: 10.1051/alr/2021014
Ayoe Hoff , Max Nielsen , Rasmus Nielsen , Satoshi Yamazaki
Theory suggests the use of individual transferable quotas (ITQs) as a solution to overcapacity and to keep efficient fishers active. While the reduction of overcapacity under ITQ implementation is well documented, empirical evidence on the role of capacity utilisation in adjusting the labour force is scarce. This article analyses whether the capacity utilisation of the vessels that fishers own/work on influences their probability of continuing fishing or whether factors such as fishing income and pension are more important. Danish small-scale fisheries with vessels less than 17 m in length, in which ITQs were introduced in 2007, are studied using a multinomial logit regression based on a unique dataset of individual income and socioeconomic characteristics of Danish fishers in the period 2002-2012 as well as individual vessel data. Together with other relevant socioeconomic variables, vessel capacity utilisation is included in the regression. The latter is identified in a productivity analysis of all commercial active vessels using Data Envelopment Analysis. It is found that increasing vessel capacity utilisation both significantly and positively influences the decision to stay in a small-scale fishery. Increasing income from fisheries also significantly influences the probability of staying in the fishery business. The Danish results provide evidence that the most efficient fishers are those who remain active when ITQs are implemented.
理论建议使用个人可转让配额(ITQs)作为解决产能过剩和保持高效渔民活跃的解决方案。虽然在实施创新技术q的过程中,产能过剩的减少得到了充分的证明,但关于产能利用在调整劳动力方面的作用的经验证据却很少。本文分析了渔民拥有/工作的船只的容量利用率是否会影响他们继续捕鱼的可能性,或者捕鱼收入和养老金等因素是否更重要。在2007年引入ITQs的丹麦小型渔业中,基于2002-2012年期间丹麦渔民的个人收入和社会经济特征的独特数据集以及个人船只数据,使用多项logit回归研究了长度小于17米的丹麦小型渔业。与其他相关的社会经济变量一起,船舶容量利用率被纳入回归。后者是在使用数据包络分析对所有商业活跃船只的生产力分析中确定的。研究发现,增加船舶容量利用率对小规模渔业的决定有显著和积极的影响。渔业收入的增加也显著影响了留在渔业行业的可能性。丹麦的研究结果提供了证据,证明效率最高的渔民是那些在实施itq时仍保持活跃的渔民。
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引用次数: 0
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Aquatic Living Resources
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