Abstract Foot and mouth disease (FMD) is one of the most contagious diseases of livestock with a significant economic impact affecting most countries in the world over the years. In Turkey, FMD is endemic, but there have not been national studies conducted to analyze spatiotemporal pattern of FMD yet. This study was carried out to identify the spatial and temporal distribution of FMD outbreaks in Turkey from January 2010 to December 2019, to guide the eradication following development of control programs against the disease. Thematic maps were produced to determine FMD sensitive regions and Box-Jenkins time series approach was used to analyze the temporal pattern of FMD. Between these dates, 6698 outbreaks and 246341 cases were reported in Turkey, FMD was recorded multiple times in 96.3% of the provinces (n = 78), and the average incidence of FMD outbreaks at the provincial level was calculated as 8.27/province year. As result of the spatial pattern of FMD, East and Central Anatolia were determined as the regions where the disease was observed intensely. The time series plot of the data showed a general not very regular trend although there was a downward trend with irregular variations. Although, there was no seasonal effect detected by the decomposition of time series, seasonal peaks in the outbreaks were observed, in the spring (n = 2087, 31.16%). In conclusion, the evaluation of spatial and temporal pattern based on FMD outbreaks that are common in Turkey will contribute to eradication of the disease.
{"title":"Spatiotemporal Distributions of Foot and Mouth Disease Between 2010-2019 in Turkey","authors":"T. Bayir, I. S. Gürcan","doi":"10.2478/acve-2022-0027","DOIUrl":"https://doi.org/10.2478/acve-2022-0027","url":null,"abstract":"Abstract Foot and mouth disease (FMD) is one of the most contagious diseases of livestock with a significant economic impact affecting most countries in the world over the years. In Turkey, FMD is endemic, but there have not been national studies conducted to analyze spatiotemporal pattern of FMD yet. This study was carried out to identify the spatial and temporal distribution of FMD outbreaks in Turkey from January 2010 to December 2019, to guide the eradication following development of control programs against the disease. Thematic maps were produced to determine FMD sensitive regions and Box-Jenkins time series approach was used to analyze the temporal pattern of FMD. Between these dates, 6698 outbreaks and 246341 cases were reported in Turkey, FMD was recorded multiple times in 96.3% of the provinces (n = 78), and the average incidence of FMD outbreaks at the provincial level was calculated as 8.27/province year. As result of the spatial pattern of FMD, East and Central Anatolia were determined as the regions where the disease was observed intensely. The time series plot of the data showed a general not very regular trend although there was a downward trend with irregular variations. Although, there was no seasonal effect detected by the decomposition of time series, seasonal peaks in the outbreaks were observed, in the spring (n = 2087, 31.16%). In conclusion, the evaluation of spatial and temporal pattern based on FMD outbreaks that are common in Turkey will contribute to eradication of the disease.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42537728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Eroksuz, M. Timurkan, B. Karabulut, C. A. Incili, E. Baydar, A. Çevik, H. Eroksuz
Abstract Caprine Arthritis-Encephalitis Virus Infection was presented with clinical, pathological, and molecular findings in dairy goats from a breeding unit in Turkey. According to history, 50 of 700 goats and kids showed the clinical signs including anorexia, mastitis, swelling of the joints, lameness, dyspnea and head pressing in the past 2 months. Fifteen and 6 animals were examined clinically and pathologically, respectively. Clinical examination revealed depression, weakness, pneumonia, ataxia and paresis in 15 goats. Histological lesions were composed by a varying degree of interstitial pneumonia, nonsuppurative leukoencephalomyelitis, interstitial nephritis and interstitial myocarditis. Immunohistochemical examinations showed the presence of viral antigen in tubular epithelium, in glial cells and macrophages. In conclusion, this is the first report documenting pathological and molecular changes of Caprine Arthritis-Encephalitis Virus Infection in Turkey in goats.
{"title":"Clinical, Pathological and Molecular Findings in Caprine Arthritis – Encephalitis Virus Infection in Damascus Goats","authors":"Y. Eroksuz, M. Timurkan, B. Karabulut, C. A. Incili, E. Baydar, A. Çevik, H. Eroksuz","doi":"10.2478/acve-2022-0026","DOIUrl":"https://doi.org/10.2478/acve-2022-0026","url":null,"abstract":"Abstract Caprine Arthritis-Encephalitis Virus Infection was presented with clinical, pathological, and molecular findings in dairy goats from a breeding unit in Turkey. According to history, 50 of 700 goats and kids showed the clinical signs including anorexia, mastitis, swelling of the joints, lameness, dyspnea and head pressing in the past 2 months. Fifteen and 6 animals were examined clinically and pathologically, respectively. Clinical examination revealed depression, weakness, pneumonia, ataxia and paresis in 15 goats. Histological lesions were composed by a varying degree of interstitial pneumonia, nonsuppurative leukoencephalomyelitis, interstitial nephritis and interstitial myocarditis. Immunohistochemical examinations showed the presence of viral antigen in tubular epithelium, in glial cells and macrophages. In conclusion, this is the first report documenting pathological and molecular changes of Caprine Arthritis-Encephalitis Virus Infection in Turkey in goats.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41665537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soong-Hee Youn, Ahmed K. Efladl, Myung-Jin Chung, Eun Jung, Ki-Yong Shin, Hyeon-Joo Shin, Joon-Young Yang, Kwang-Seok Heo, Dongmin Chung, Jae-Hyuk Yim, J. Son, Eun-Joo Lee, I. Hong, K. Jeong
Abstract Chronic renal failure is one of the leading causes of death in African lions, cheetahs, and tigers. Conventional methods to measure renal dysfunction include measuring serum creatinine and blood urea nitrogen (BUN). Symmetric dimethylarginine (SDMA) measurement is a reliable predictor of renal dysfunction in the domestic cat because SDMA serum levels increase early when the kidneys are damaged. Serum SDMA levels were assessed and correlated with creatinine as well as BUN from healthy captive Bengal tigers and lions at the Everland Zoo in Korea. Serum SDMA concentrations were increasingly associated with increased age in lions. However, SDMA concentrations were higher in some young Bengal tigers than in older ones, which may allow for earlier renal dysfunction detection in these young cats than would be BUN and creatinine alone. In Bengal tigers, the correlation between the SDMA and BUN was slightly higher than that between SDMA and creatinine. In lions, SDMA correlated better with creatinine than with BUN concentration. These results show that serum SDMA concentration can be used as a biomarker for age-associated renal dysfunction. SDMA measurement may be an essential preventive management method in zoos.
{"title":"Symmetric Dimethylarginine is a Promising Biomarker for the Early Detection of Age-Related Kidney Dysfunction in Zoo Felids","authors":"Soong-Hee Youn, Ahmed K. Efladl, Myung-Jin Chung, Eun Jung, Ki-Yong Shin, Hyeon-Joo Shin, Joon-Young Yang, Kwang-Seok Heo, Dongmin Chung, Jae-Hyuk Yim, J. Son, Eun-Joo Lee, I. Hong, K. Jeong","doi":"10.2478/acve-2022-0033","DOIUrl":"https://doi.org/10.2478/acve-2022-0033","url":null,"abstract":"Abstract Chronic renal failure is one of the leading causes of death in African lions, cheetahs, and tigers. Conventional methods to measure renal dysfunction include measuring serum creatinine and blood urea nitrogen (BUN). Symmetric dimethylarginine (SDMA) measurement is a reliable predictor of renal dysfunction in the domestic cat because SDMA serum levels increase early when the kidneys are damaged. Serum SDMA levels were assessed and correlated with creatinine as well as BUN from healthy captive Bengal tigers and lions at the Everland Zoo in Korea. Serum SDMA concentrations were increasingly associated with increased age in lions. However, SDMA concentrations were higher in some young Bengal tigers than in older ones, which may allow for earlier renal dysfunction detection in these young cats than would be BUN and creatinine alone. In Bengal tigers, the correlation between the SDMA and BUN was slightly higher than that between SDMA and creatinine. In lions, SDMA correlated better with creatinine than with BUN concentration. These results show that serum SDMA concentration can be used as a biomarker for age-associated renal dysfunction. SDMA measurement may be an essential preventive management method in zoos.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47810663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Suárez-Cabrera, M. Encinoso, A. Caraballo, M. Morales, J. A. Corbera, Francisco Rodríguez-Guisado, C. Melián, J. R. Jáber
Abstract A one-year-old European shorthair feral cat with signs of heat was presented at the Veterinary Teaching Hospital of Las Palmas de Gran Canaria University. After the physical exam, histology, hormonal analysis, ultrasound and computed tomography (CT) studies were performed. Examination of the external genitalia revealed the absence of one of the testes in the scrotal sac and the presence of a structure whose appearance could suggest an enlarged penis-like clitoris with small spines. The ultrasound study showed compatible images with the left ovary and intra-abdominal testis and tubular structures, which closely resembled the uterine horns. Similar structures were confirmed in the CT study. Exploratory celiotomy revealed the presence of the uterus and undescended testis attached to the uterine horn. Histological examination revealed immature testicular tissue in both gonads and the presence of Persistent Müllerian Duct (PMD). Though a karyotype was not performed, it was presumed to be the standard 38 XY found in pseudohermaphrodites. To date, this type of disorder of sexual development (DSD) has been scarcely reported in cats. Further studies are on the way to knowing the genetic mechanism of this disease.
{"title":"Morphological and Imaging Features of Male Pseudohermaphroditism in a Feral Cat","authors":"F. Suárez-Cabrera, M. Encinoso, A. Caraballo, M. Morales, J. A. Corbera, Francisco Rodríguez-Guisado, C. Melián, J. R. Jáber","doi":"10.2478/acve-2022-0032","DOIUrl":"https://doi.org/10.2478/acve-2022-0032","url":null,"abstract":"Abstract A one-year-old European shorthair feral cat with signs of heat was presented at the Veterinary Teaching Hospital of Las Palmas de Gran Canaria University. After the physical exam, histology, hormonal analysis, ultrasound and computed tomography (CT) studies were performed. Examination of the external genitalia revealed the absence of one of the testes in the scrotal sac and the presence of a structure whose appearance could suggest an enlarged penis-like clitoris with small spines. The ultrasound study showed compatible images with the left ovary and intra-abdominal testis and tubular structures, which closely resembled the uterine horns. Similar structures were confirmed in the CT study. Exploratory celiotomy revealed the presence of the uterus and undescended testis attached to the uterine horn. Histological examination revealed immature testicular tissue in both gonads and the presence of Persistent Müllerian Duct (PMD). Though a karyotype was not performed, it was presumed to be the standard 38 XY found in pseudohermaphrodites. To date, this type of disorder of sexual development (DSD) has been scarcely reported in cats. Further studies are on the way to knowing the genetic mechanism of this disease.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44701245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Winter is the season that poses the greatest challenges for honey bee colonies. Therefore, the main approach in beekeeping practice is aimed mainly at providing sufficient quality food supplies for bee colonies in early autumn. We conducted the present study to test the influence of the natural plant extract IMМUNOSTART HERB on population strength, stored pollen area, capped worker brood area, and honey yield. The experimental groups were supplied with IMМUNOSTART HERB 4 times at 7-day intervals, whereas sugar syrup was given to the control groups. The obtained results showed that the applied supplemental diet affected all investigated biological parameters, with the most noticeable effect after the second application. In all measurements, the honey bee colony parameters in the treated groups showed higher values in comparison to the control groups. These results highlight the potential of herbal supplements to effectively improve bee colonies’ development during the period of scarce bee forage, as well as to provide suitable conditions for successful overwintering.
冬季是蜜蜂群体面临最大挑战的季节。因此，养蜂实践的主要方法主要是在初秋为蜂群提供充足的优质食物供应。本研究考察了天然植物提取物IMМUNOSTART HERB对蜂群强度、花粉储存面积、工蜂顶巢面积和蜂蜜产量的影响。试验组饲喂IMМUNOSTART HERB 4次，每7 d一次，对照组饲喂糖浆。结果表明，添加饲粮对所研究的各项生物学参数均有影响，以第二次添加后影响最为显著。在所有测量中，治疗组的蜂群参数显示出比对照组更高的值。这些结果表明，在蜜蜂饲料稀缺时期，草药补充剂可以有效地改善蜂群的发育，并为成功越冬提供适宜的条件。
{"title":"Impact of the Plant-Based Natural Supplement Imмunostart Herb on Honey Bee Colony Performance","authors":"R. Shumkova, R. Balkanska, D. Salkova, P. Hristov","doi":"10.2478/acve-2022-0028","DOIUrl":"https://doi.org/10.2478/acve-2022-0028","url":null,"abstract":"Abstract Winter is the season that poses the greatest challenges for honey bee colonies. Therefore, the main approach in beekeeping practice is aimed mainly at providing sufficient quality food supplies for bee colonies in early autumn. We conducted the present study to test the influence of the natural plant extract IMМUNOSTART HERB on population strength, stored pollen area, capped worker brood area, and honey yield. The experimental groups were supplied with IMМUNOSTART HERB 4 times at 7-day intervals, whereas sugar syrup was given to the control groups. The obtained results showed that the applied supplemental diet affected all investigated biological parameters, with the most noticeable effect after the second application. In all measurements, the honey bee colony parameters in the treated groups showed higher values in comparison to the control groups. These results highlight the potential of herbal supplements to effectively improve bee colonies’ development during the period of scarce bee forage, as well as to provide suitable conditions for successful overwintering.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43650416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Platelet-rich plasma ( PRP), an autologous platelet concentrated in plasma, is a source of diverse growth factors and is extensively utilized to promote tissue healing. Most of the clinical and laboratory investigations in veterinary medicine have focused on horses and dogs. Consequently, the types and detailed conditions of the PRP preparation method are based on those species. However, in bovine medicine, only a few studies have investigated the species-specific characteristics of bovine platelets. The aim of this study was to optimize the conditions of a two-step centrifugation method for bovine PRP. Whole blood samples were obtained from eight healthy lactating Holstein-Friesian cows. Eight running conditions for the first and second centrifugations each were evaluated based on the platelet recovery rate, the concentration of growth factors, and the adenosine diphosphate (ADP)-induced aggregation response, which reflects the premature platelet activation caused by the centrifugation process. The results of this study showed that for the first and second centrifugations, 900×g for 10 minutes and 1250×g for 15 minutes, respectively, were the most suitable conditions for the platelet recovery rate, and the concentration of growth factors was highest under these conditions without significant activation of premature platelet aggregation. Thus, we established an optimal two-step centrifugation protocol for bovine PRP that should provide a better understanding of bovine platelets.
{"title":"Optimization of a Two-Step Centrifugation Protocol for Bovine Platelet-Rich Plasma","authors":"Mu-Young Kim, Hyun-Jung Han","doi":"10.2478/acve-2022-0030","DOIUrl":"https://doi.org/10.2478/acve-2022-0030","url":null,"abstract":"Abstract Platelet-rich plasma ( PRP), an autologous platelet concentrated in plasma, is a source of diverse growth factors and is extensively utilized to promote tissue healing. Most of the clinical and laboratory investigations in veterinary medicine have focused on horses and dogs. Consequently, the types and detailed conditions of the PRP preparation method are based on those species. However, in bovine medicine, only a few studies have investigated the species-specific characteristics of bovine platelets. The aim of this study was to optimize the conditions of a two-step centrifugation method for bovine PRP. Whole blood samples were obtained from eight healthy lactating Holstein-Friesian cows. Eight running conditions for the first and second centrifugations each were evaluated based on the platelet recovery rate, the concentration of growth factors, and the adenosine diphosphate (ADP)-induced aggregation response, which reflects the premature platelet activation caused by the centrifugation process. The results of this study showed that for the first and second centrifugations, 900×g for 10 minutes and 1250×g for 15 minutes, respectively, were the most suitable conditions for the platelet recovery rate, and the concentration of growth factors was highest under these conditions without significant activation of premature platelet aggregation. Thus, we established an optimal two-step centrifugation protocol for bovine PRP that should provide a better understanding of bovine platelets.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47572301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Mammary gland tumor is rare and only a few cases have been reported in wild animals. Moreover, most etiologies of the known cases in veterinary medicine are related to age, diet, obesity, and excessive sex hormones in domestic animals while few etiologies are reported in wild animals. An unknown-aged female wild boar was presented to the Department of Veterinary Pathology, Kyungpook National University with a well-demarcated, and firm-to-elastic mammary gland mass. The cut-surface of the mass was pink-reddish, and homogeneous. Microscopically, the mass was mainly composed of well-differentiated neoplastic glandular epithelial cells characterized by a single-layer, columnar to cuboidal shapes, and small and central nuclei and nucleoli. Any evidence of invasiveness or metastases of the neoplastic cells were not observed. Interestingly, infiltration of chronic inflammatory cells such as plasma cells and macrophages was observed along with a large quantity of gram-negative and positive bacterial colonies in the mammary glands. Moreover, accompanied fibrosis of stroma was observed, as well. Based on the gross and microscopic findings, the present case was diagnosed as mammary simple adenoma caused by chronic mastitis progressing to fibrotic condition. To the authors’ knowledge, this is the first study describing the histopathological aspects of mammary gland tumors associated with chronic mastitis accompanied by fibrosis in wild animals.
{"title":"Mammary Adenoma Associated with Chronic Mastitis in a Wild Boar (Sus Scrofa)","authors":"Su-Min Baek, Seoung-Woo Lee, Jin-Kyu Park","doi":"10.2478/acve-2022-0031","DOIUrl":"https://doi.org/10.2478/acve-2022-0031","url":null,"abstract":"Abstract Mammary gland tumor is rare and only a few cases have been reported in wild animals. Moreover, most etiologies of the known cases in veterinary medicine are related to age, diet, obesity, and excessive sex hormones in domestic animals while few etiologies are reported in wild animals. An unknown-aged female wild boar was presented to the Department of Veterinary Pathology, Kyungpook National University with a well-demarcated, and firm-to-elastic mammary gland mass. The cut-surface of the mass was pink-reddish, and homogeneous. Microscopically, the mass was mainly composed of well-differentiated neoplastic glandular epithelial cells characterized by a single-layer, columnar to cuboidal shapes, and small and central nuclei and nucleoli. Any evidence of invasiveness or metastases of the neoplastic cells were not observed. Interestingly, infiltration of chronic inflammatory cells such as plasma cells and macrophages was observed along with a large quantity of gram-negative and positive bacterial colonies in the mammary glands. Moreover, accompanied fibrosis of stroma was observed, as well. Based on the gross and microscopic findings, the present case was diagnosed as mammary simple adenoma caused by chronic mastitis progressing to fibrotic condition. To the authors’ knowledge, this is the first study describing the histopathological aspects of mammary gland tumors associated with chronic mastitis accompanied by fibrosis in wild animals.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44067124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Đorđević, J. Blagojević, M. Radinović, M. Cincović, J. Maletić, M. Kreszinger, Milan Maletic
Abstract The reproductive efficiency of the cows was monitored after two resynchronization protocols: Ovsynch (OVS) and Double Ovsynch (DOS). The research initially included 70 HF cows who entered the first synchronization protocol – Presynch. Cows that did not conceive after the first synchronization were divided into two groups and introduced to two resynchronization protocols. In the first group of cows (n=35), the DOS protocol began with the application of GnRH on day 22 after the Presynch TAI (Timed Artificial Insemination), and seven days later pregnancy check-up was done and PGF2α was applied only to non-pregnant cows (n=23), which remained in the study. In the second group of cows, the OVS protocol started on day 32 after Presynch TAI only in non-pregnant animals (n=20). Progesterone (P4) concentration was determined at the time of application of GnRH1, PGF2α and GnRH2 in both groups of cows, and then 30 days after Resynch TAI, ultrasound pregnancy diagnosis was done. A higher percentage of pregnant cows were recorded in the OVS group compared to the DOS group (45% and 35%, respectively). The concentration of P4 in the serum of cows in the DOS group during the first measurement (GnRH1) was significantly higher than the value in cows that did not conceive (p<0.05), while in the third measurement (GnRH2) the average concentration of P4 in conceiving cows was significantly lower (p<0.001) compared to cows that did not coincive. The open days period was significantly longer in pregnant cows that were resynchronized using the DOS protocol compared to cows from the OVS protocol. In conclusion, the OVS protocol of estrus resynchronization in dairy cows proved to be more successful than the DOS protocol. However, considering the advantages the OVS, it is needed to determine which day of the sexual cycle is the best to start resynchronization.
{"title":"Efficiency of Two Protocols of Resynchronization of Estrus and Ovulation in High-Producing Dairy Cows at Peak Lactation","authors":"M. Đorđević, J. Blagojević, M. Radinović, M. Cincović, J. Maletić, M. Kreszinger, Milan Maletic","doi":"10.2478/acve-2022-0029","DOIUrl":"https://doi.org/10.2478/acve-2022-0029","url":null,"abstract":"Abstract The reproductive efficiency of the cows was monitored after two resynchronization protocols: Ovsynch (OVS) and Double Ovsynch (DOS). The research initially included 70 HF cows who entered the first synchronization protocol – Presynch. Cows that did not conceive after the first synchronization were divided into two groups and introduced to two resynchronization protocols. In the first group of cows (n=35), the DOS protocol began with the application of GnRH on day 22 after the Presynch TAI (Timed Artificial Insemination), and seven days later pregnancy check-up was done and PGF2α was applied only to non-pregnant cows (n=23), which remained in the study. In the second group of cows, the OVS protocol started on day 32 after Presynch TAI only in non-pregnant animals (n=20). Progesterone (P4) concentration was determined at the time of application of GnRH1, PGF2α and GnRH2 in both groups of cows, and then 30 days after Resynch TAI, ultrasound pregnancy diagnosis was done. A higher percentage of pregnant cows were recorded in the OVS group compared to the DOS group (45% and 35%, respectively). The concentration of P4 in the serum of cows in the DOS group during the first measurement (GnRH1) was significantly higher than the value in cows that did not conceive (p<0.05), while in the third measurement (GnRH2) the average concentration of P4 in conceiving cows was significantly lower (p<0.001) compared to cows that did not coincive. The open days period was significantly longer in pregnant cows that were resynchronized using the DOS protocol compared to cows from the OVS protocol. In conclusion, the OVS protocol of estrus resynchronization in dairy cows proved to be more successful than the DOS protocol. However, considering the advantages the OVS, it is needed to determine which day of the sexual cycle is the best to start resynchronization.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46884208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Tamburro, A. Pratesi, Fabio Carli, F. Collivignarelli, Amanda Bianchi, Andrea Paolini, Ilaria Falerno, Andrea De Bonis, M. Vignoli
Abstract The aim of this retrospective study was to evaluate the clinical and radiographic outcomes of 21 cats that underwent coxofemoral toggle stabilization using the Mini TightRope® Fixation System (mTR®). Data were compiled from client-owned cats with traumatic hip luxation. Each cat underwent the same protocol including orthopedic assessment, preoperative radiographic evaluation, surgical procedure, postoperative management and clinical re-examination at 14 days, 8 weeks and 12 months following surgery. Age, body weight, implants and osteoarthritis progression were recorded. A lameness score 0-4 was assigned to each cat. Pain upon hip extension was not quantified but as pain reaction or no pain reaction. Major complications and osteoarthritic (OA) changes were recorded. Osteoarthritic changes were classified on a 0-4 scale. Twenty-one cats were included in the study. Coxofemoral luxations were stabilized using a mTR®. At 8 weeks, weight bearing was considered normal (grade 0) in all cats and no pain reaction was elicited during hip extension. One year after surgery, orthopedic examination was within the normal limits and according to the owners, all animals exhibited a normal physical activity. No complications were recorded. Evidence of slight OA changes were observed in 5 out of 21 cats. The final outcome was considered excellent in 16/21 patients and good in 5/21 cats. The application of the mTR® system can be considered a suitable surgical treatment in cats affected by traumatic hip luxation.
{"title":"Traumatic Coxofemoral Luxation in Cats Treated with Hip-Toggle Stabilization Using the Mini Tightrope® Fixation System","authors":"R. Tamburro, A. Pratesi, Fabio Carli, F. Collivignarelli, Amanda Bianchi, Andrea Paolini, Ilaria Falerno, Andrea De Bonis, M. Vignoli","doi":"10.2478/acve-2022-0024","DOIUrl":"https://doi.org/10.2478/acve-2022-0024","url":null,"abstract":"Abstract The aim of this retrospective study was to evaluate the clinical and radiographic outcomes of 21 cats that underwent coxofemoral toggle stabilization using the Mini TightRope® Fixation System (mTR®). Data were compiled from client-owned cats with traumatic hip luxation. Each cat underwent the same protocol including orthopedic assessment, preoperative radiographic evaluation, surgical procedure, postoperative management and clinical re-examination at 14 days, 8 weeks and 12 months following surgery. Age, body weight, implants and osteoarthritis progression were recorded. A lameness score 0-4 was assigned to each cat. Pain upon hip extension was not quantified but as pain reaction or no pain reaction. Major complications and osteoarthritic (OA) changes were recorded. Osteoarthritic changes were classified on a 0-4 scale. Twenty-one cats were included in the study. Coxofemoral luxations were stabilized using a mTR®. At 8 weeks, weight bearing was considered normal (grade 0) in all cats and no pain reaction was elicited during hip extension. One year after surgery, orthopedic examination was within the normal limits and according to the owners, all animals exhibited a normal physical activity. No complications were recorded. Evidence of slight OA changes were observed in 5 out of 21 cats. The final outcome was considered excellent in 16/21 patients and good in 5/21 cats. The application of the mTR® system can be considered a suitable surgical treatment in cats affected by traumatic hip luxation.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48962396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Milutin Antić, A. Beletić, M. Radaković, Kristina Spariosu, S. Stanković, D. Trailović, M. Kovačević Filipović
Abstract Progressive tissue injury in canine leishmaniosis (CL) is related to the deposition of immune complexes, which induces vasculitis and leads to endothelial dysfunction. Homocysteine (Hcy) increase may worsen endothelial dysfunction, but data concerning its concentration in different CL stages and links to the acute phase response and oxidative stress are missing. We compared Hcy levels between dogs with mild (N=24) and moderate CL without treatment (N=17) and treated with anti-Leishmania drugs and vitamin B supplements (N=9). Dogs with moderate CL, regardless of therapy administration, had more distinct clinical signs, lower erythron values, and a higher level of acute-phase proteins (APPs), IgG against Leishmania spp., urea and creatinine, than dogs with mild CL. Hcy values did not differ between stages, but treated dogs had the lowest levels of Hcy. An inverse relationship existed between Hcy and the CL stage, therapy, levels of IgG, and clinical pathology data. The only positive relationship existed between Hcy and the erythron state. The disease stage and therapeutic intervention were not related to the oxidative stress level, except in the case of paraoxonase-1/Hcy ratio, indicating favorable conditions for antioxidative defense in treated dogs. In conclusion, changes in Hcy levels indicated its possible involvement with endothelial dysfunction and inverse relationship to tissue injury evaluated by APPs. Finally, Hcy might be an early marker of favorable conditions for endothelium recovery in CL.
{"title":"Homocysteine Concentration in the Serum of Dogs Naturally Infected with Leishmania Spp. – Association with the Stage of the Disease, Therapy, and Clinical Pathology Data","authors":"Milutin Antić, A. Beletić, M. Radaković, Kristina Spariosu, S. Stanković, D. Trailović, M. Kovačević Filipović","doi":"10.2478/acve-2022-0025","DOIUrl":"https://doi.org/10.2478/acve-2022-0025","url":null,"abstract":"Abstract Progressive tissue injury in canine leishmaniosis (CL) is related to the deposition of immune complexes, which induces vasculitis and leads to endothelial dysfunction. Homocysteine (Hcy) increase may worsen endothelial dysfunction, but data concerning its concentration in different CL stages and links to the acute phase response and oxidative stress are missing. We compared Hcy levels between dogs with mild (N=24) and moderate CL without treatment (N=17) and treated with anti-Leishmania drugs and vitamin B supplements (N=9). Dogs with moderate CL, regardless of therapy administration, had more distinct clinical signs, lower erythron values, and a higher level of acute-phase proteins (APPs), IgG against Leishmania spp., urea and creatinine, than dogs with mild CL. Hcy values did not differ between stages, but treated dogs had the lowest levels of Hcy. An inverse relationship existed between Hcy and the CL stage, therapy, levels of IgG, and clinical pathology data. The only positive relationship existed between Hcy and the erythron state. The disease stage and therapeutic intervention were not related to the oxidative stress level, except in the case of paraoxonase-1/Hcy ratio, indicating favorable conditions for antioxidative defense in treated dogs. In conclusion, changes in Hcy levels indicated its possible involvement with endothelial dysfunction and inverse relationship to tissue injury evaluated by APPs. Finally, Hcy might be an early marker of favorable conditions for endothelium recovery in CL.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43982249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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