T. Popović, Jasmina Debeljak Martačić, Biljana Pokimica, Branko Ravić, Slavica Ranković, M. Glibetić, P. Stepanović
Abstract Intake of long-chain n-3 polyunsaturated fatty acids (PUFA) benefits human and animal health. Our study aimed to analyze the long-chain n-3 PUFA content of two types of food and their effect on plasma and erythrocyte phospholipids of Belgian Shepherd dogs. A total of 10 dogs were fed commercial granulated food (Food 1), and another 10 were provided commercial Premium granulated food of high quality (Food 2). All the analyses were performed using gas-liquid chromatography. Our results showed that Food 1 contained more n-3 PUFA than Food 2, which was reflected in higher n-3 PUFA in plasma and erythrocyte phospholipids. Because long-chain n-3 PUFA in phospholipids are precursors for antioxidative molecules, further studies should investigate the effects of the analyzed commercial granulated food rich in n-3 on oxidative stress parameters in dogs.
{"title":"Phospholipid Fatty Acid Profiles of Plasma and Erythrocyte Membranes in Dogs Fed with Commercial Granulated Food","authors":"T. Popović, Jasmina Debeljak Martačić, Biljana Pokimica, Branko Ravić, Slavica Ranković, M. Glibetić, P. Stepanović","doi":"10.2478/acve-2023-0009","DOIUrl":"https://doi.org/10.2478/acve-2023-0009","url":null,"abstract":"Abstract Intake of long-chain n-3 polyunsaturated fatty acids (PUFA) benefits human and animal health. Our study aimed to analyze the long-chain n-3 PUFA content of two types of food and their effect on plasma and erythrocyte phospholipids of Belgian Shepherd dogs. A total of 10 dogs were fed commercial granulated food (Food 1), and another 10 were provided commercial Premium granulated food of high quality (Food 2). All the analyses were performed using gas-liquid chromatography. Our results showed that Food 1 contained more n-3 PUFA than Food 2, which was reflected in higher n-3 PUFA in plasma and erythrocyte phospholipids. Because long-chain n-3 PUFA in phospholipids are precursors for antioxidative molecules, further studies should investigate the effects of the analyzed commercial granulated food rich in n-3 on oxidative stress parameters in dogs.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46422130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dušan Bošnjaković, D. Kirovski, R. Prodanović, I. Vujanac, Sveta Arsić, Milica Stojković, Slavica Dražić, Sreten Nedić, Ljubomir Jovanović
Abstract Ruminant husbandry contributes to global methane (CH4) emissions and beside its negative impact on the environment, enteric CH4 emissions cause a loss of gross energy intake in cows. The study is aimed to estimate CH4 emission and metabolic status in dairy cows via the methane concentration profile as a tool for analyzing the CH4 production pattern. The study included eighteen cows whose enteric CH4 emission was measured during three consecutive days in three periods: 2 hours before (P1), 2–4 hours (P2) and 6–8 hours (P3) after the morning feeding. Based on CH4 enteric emissions, cows were divided into two groups (n=6, respectively): HM (average CH4 concentration: 5430.08 ± 365.92 ppm) and LM (average CH4 concentration: 1351.85 ± 205.20 ppm). Following CH4 measurement, on day 3, venous blood was sampled to determine the indicators of the metabolic status. HM cows had significantly higher average CH4 concentrations, maximum and average CH4 peak amplitude than LM cows in all measuring periods (P1-P3), while the number of CH4 peaks tended to be higher in HM than in LM cows in P2. There were no differences in the maximum and average CH4 peak width and average distance among two CH4 peaks between examined groups of cows. HM cows had significantly higher total protein concentrations and significantly lower total bilirubin and NEFA concentrations than LM cows. In conclusion, HM cows have a greater number of eructations and release more CH4 per eructation than LM cows, hence the differences in metabolic status are most likely related to the differences in their liver function.
{"title":"Methane Emission and Metabolic Status in Peak Lactating Dairy Cows and Their Assessment Via Methane Concentration Profile","authors":"Dušan Bošnjaković, D. Kirovski, R. Prodanović, I. Vujanac, Sveta Arsić, Milica Stojković, Slavica Dražić, Sreten Nedić, Ljubomir Jovanović","doi":"10.2478/acve-2023-0006","DOIUrl":"https://doi.org/10.2478/acve-2023-0006","url":null,"abstract":"Abstract Ruminant husbandry contributes to global methane (CH4) emissions and beside its negative impact on the environment, enteric CH4 emissions cause a loss of gross energy intake in cows. The study is aimed to estimate CH4 emission and metabolic status in dairy cows via the methane concentration profile as a tool for analyzing the CH4 production pattern. The study included eighteen cows whose enteric CH4 emission was measured during three consecutive days in three periods: 2 hours before (P1), 2–4 hours (P2) and 6–8 hours (P3) after the morning feeding. Based on CH4 enteric emissions, cows were divided into two groups (n=6, respectively): HM (average CH4 concentration: 5430.08 ± 365.92 ppm) and LM (average CH4 concentration: 1351.85 ± 205.20 ppm). Following CH4 measurement, on day 3, venous blood was sampled to determine the indicators of the metabolic status. HM cows had significantly higher average CH4 concentrations, maximum and average CH4 peak amplitude than LM cows in all measuring periods (P1-P3), while the number of CH4 peaks tended to be higher in HM than in LM cows in P2. There were no differences in the maximum and average CH4 peak width and average distance among two CH4 peaks between examined groups of cows. HM cows had significantly higher total protein concentrations and significantly lower total bilirubin and NEFA concentrations than LM cows. In conclusion, HM cows have a greater number of eructations and release more CH4 per eructation than LM cows, hence the differences in metabolic status are most likely related to the differences in their liver function.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46044547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jernej Šmigoc, Katarina Pavšič Vrtač, Breda Jakovac Strajn, Mateja Stvarnik, J. Mrkun
Abstract The effects of stress on processes in the body are becoming an increasingly relevant research subject. The reproductive ability of bovine animals largely depends on these effects, whilst embryo transfer is increasingly being used as a reproduction method. In this study, we established the differences in the implantation ability of heifers that were treated (N=17) with selenium (Se) and vitamins AD3E, and non-treated heifers. Upon transfer, we took blood samples from both groups and used the total antioxidant status (TAS) value to analyze the presence of reactive oxygen species (ROS), the levels of non-esterified fatty acids (NEFA) and the levels of vitamin E and Se in blood plasma. In the study, we were able to demonstrate that preventive measures in the form of supplementation of vitamin E and Se, mitigate the effects of oxidative stress, strengthen the ability of an organism to improve the dynamic relationship between free radicals and antioxidants, improve the energy status of cattle, positively impact reproductive parameters and increase the success rate of embryo transfer. The difference in the number of successful embryo implantations between the control and treated group was statistically significant, with 64.7% of treated heifers being pregnant after embryo transfer and giving birth to healthy calves. In the control group, the implantation success rate was 41.2%. The supplementation of antioxidants in the form of a combination of vitamin AD3E and Se, proved to be a good method for strengthening the defense of an organism and an effective mean of preventive clinical approach for improving fertility parameters.
{"title":"Preventive Supplementation of Vitamin E and Selenium as a Factor in Improving the Success Rate of Embryo Transfer in Cattle","authors":"Jernej Šmigoc, Katarina Pavšič Vrtač, Breda Jakovac Strajn, Mateja Stvarnik, J. Mrkun","doi":"10.2478/acve-2023-0007","DOIUrl":"https://doi.org/10.2478/acve-2023-0007","url":null,"abstract":"Abstract The effects of stress on processes in the body are becoming an increasingly relevant research subject. The reproductive ability of bovine animals largely depends on these effects, whilst embryo transfer is increasingly being used as a reproduction method. In this study, we established the differences in the implantation ability of heifers that were treated (N=17) with selenium (Se) and vitamins AD3E, and non-treated heifers. Upon transfer, we took blood samples from both groups and used the total antioxidant status (TAS) value to analyze the presence of reactive oxygen species (ROS), the levels of non-esterified fatty acids (NEFA) and the levels of vitamin E and Se in blood plasma. In the study, we were able to demonstrate that preventive measures in the form of supplementation of vitamin E and Se, mitigate the effects of oxidative stress, strengthen the ability of an organism to improve the dynamic relationship between free radicals and antioxidants, improve the energy status of cattle, positively impact reproductive parameters and increase the success rate of embryo transfer. The difference in the number of successful embryo implantations between the control and treated group was statistically significant, with 64.7% of treated heifers being pregnant after embryo transfer and giving birth to healthy calves. In the control group, the implantation success rate was 41.2%. The supplementation of antioxidants in the form of a combination of vitamin AD3E and Se, proved to be a good method for strengthening the defense of an organism and an effective mean of preventive clinical approach for improving fertility parameters.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48637519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Sprygin, A. Nesterov, I. Shumilova, P. Prutnikov, N. Vasković, O. Byadovskaya
Abstract In this study we report the testing of blood-feeding and synanthropic flies captured near animals affected by lumpy skin disease virus (LSDV) in Russia during the outbreaks in the Kurgan region in 2018 and Saratov region in 2019. The insects of interest were the stable fly Stomoxys calcitrans, Tabanidae horse flies, Culicoides midges and the house fly Musca domestica examined as individuals or pools. The obtained findings demonstrate that viral DNA was found in pools of S.calcitrans and M. domestica and in the head and abdomen of stable flies. This is the first report of LSDV DNA detection in Tabanidae flies from the field. The presented data are envisaged to help further guide the search for putative vectors of LSDV in different climatic regions and interpret laboratory-controlled experiments on vector-borne transmission of LSDV.
{"title":"Evidence of Lumpy Skin Virus DNA in Blood-Feeding Flies During Outbreaks in Russia in 2018-2019","authors":"A. Sprygin, A. Nesterov, I. Shumilova, P. Prutnikov, N. Vasković, O. Byadovskaya","doi":"10.2478/acve-2022-0039","DOIUrl":"https://doi.org/10.2478/acve-2022-0039","url":null,"abstract":"Abstract In this study we report the testing of blood-feeding and synanthropic flies captured near animals affected by lumpy skin disease virus (LSDV) in Russia during the outbreaks in the Kurgan region in 2018 and Saratov region in 2019. The insects of interest were the stable fly Stomoxys calcitrans, Tabanidae horse flies, Culicoides midges and the house fly Musca domestica examined as individuals or pools. The obtained findings demonstrate that viral DNA was found in pools of S.calcitrans and M. domestica and in the head and abdomen of stable flies. This is the first report of LSDV DNA detection in Tabanidae flies from the field. The presented data are envisaged to help further guide the search for putative vectors of LSDV in different climatic regions and interpret laboratory-controlled experiments on vector-borne transmission of LSDV.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46518239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Rabbit hemorrhagic disease (RHD) is a highly contagious viral disease that causes fatal acute hepatitis in domestic and wild lagomorphs. It has taken on major economic importance in countries like Morocco. In addition to the classical virus (RHDV), a novel emerged genotype (RHDV2) is circulating, especially in the north shore of the Mediterranean basin since 2010. Many small animal farmers reported clinical cases from several rabbitries in Agadir (Morocco) despite systematic vaccination against the RHDV. The main objective was to characterize the current RHDV strains circulating in the studied area to help to choose an adequate vaccine. For that, we extracted viral RNA from rabbit livers, carried out the PCR analyses, and we sequenced the viral structural capsid protein (VP60) of the RHDV. The phylogenetic analysis results allowed us to state that the novel genotype (RHDV2) is circulating in the studied geographical area, and to characterize the isolated sequences. As a conclusion, we recommend updating RHD epidemiological relating data and reviewing the vaccine protocols by both targeting RHDV (GI.1) and RHDV2 (GI.2) in any future preventive program.
{"title":"Confirmation of the Rabbit Hemorrhagic Disease Virus Type 2 (GI.2) Circulation in North Africa","authors":"Aissa Saidi, Abdelouahed Aboutaib","doi":"10.2478/acve-2022-0035","DOIUrl":"https://doi.org/10.2478/acve-2022-0035","url":null,"abstract":"Abstract Rabbit hemorrhagic disease (RHD) is a highly contagious viral disease that causes fatal acute hepatitis in domestic and wild lagomorphs. It has taken on major economic importance in countries like Morocco. In addition to the classical virus (RHDV), a novel emerged genotype (RHDV2) is circulating, especially in the north shore of the Mediterranean basin since 2010. Many small animal farmers reported clinical cases from several rabbitries in Agadir (Morocco) despite systematic vaccination against the RHDV. The main objective was to characterize the current RHDV strains circulating in the studied area to help to choose an adequate vaccine. For that, we extracted viral RNA from rabbit livers, carried out the PCR analyses, and we sequenced the viral structural capsid protein (VP60) of the RHDV. The phylogenetic analysis results allowed us to state that the novel genotype (RHDV2) is circulating in the studied geographical area, and to characterize the isolated sequences. As a conclusion, we recommend updating RHD epidemiological relating data and reviewing the vaccine protocols by both targeting RHDV (GI.1) and RHDV2 (GI.2) in any future preventive program.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42345030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Battilani, Elisa Kaehler, Alessandro Tirolo, A. Balboni, F. Dondi
Abstract This retrospective study aimed to evaluate the clinicopathological changes in a population of cats tested for feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV), in an Italian Veterinary University Hospital, in the period between January 2002 and May 2016. During the period of 14 years, 1834 cats were tested, and of these 241/1834 (13.1%) were positive for FIV antibodies and 92/1834 (5%) cats were positive for FeLV antigen. These data confirm the presence of a high prevalence of these viruses on Italian territory. To the authors’ knowledge, this study describes findings that have never been evaluated before, such as iron status in retrovirus-infected cats and urinalysis in FeLV-positive cats. In this study, FIV-positive cats were more likely to have higher serum protein concentration and lower albumin-globulin ratio than other groups of cats. Lower urine specific gravity and higher urine protein to creatinine ratio were also detected for FIV-positive cats when compared with negative and healthy cats. FeLV-positive cats were more likely to have cytopenia, decreased haemoglobin, haematocrit and RBC compared with other groups of cats. The data obtained underline the importance of considering retroviral infections in the presence of a broad spectrum of risk factors and laboratory anomalies.
{"title":"Clinicopathological Findings in Cats Tested for Feline Immunodeficiency Virus (FIV) and Feline Leukaemia Virus (FeLV)","authors":"M. Battilani, Elisa Kaehler, Alessandro Tirolo, A. Balboni, F. Dondi","doi":"10.2478/acve-2022-0034","DOIUrl":"https://doi.org/10.2478/acve-2022-0034","url":null,"abstract":"Abstract This retrospective study aimed to evaluate the clinicopathological changes in a population of cats tested for feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV), in an Italian Veterinary University Hospital, in the period between January 2002 and May 2016. During the period of 14 years, 1834 cats were tested, and of these 241/1834 (13.1%) were positive for FIV antibodies and 92/1834 (5%) cats were positive for FeLV antigen. These data confirm the presence of a high prevalence of these viruses on Italian territory. To the authors’ knowledge, this study describes findings that have never been evaluated before, such as iron status in retrovirus-infected cats and urinalysis in FeLV-positive cats. In this study, FIV-positive cats were more likely to have higher serum protein concentration and lower albumin-globulin ratio than other groups of cats. Lower urine specific gravity and higher urine protein to creatinine ratio were also detected for FIV-positive cats when compared with negative and healthy cats. FeLV-positive cats were more likely to have cytopenia, decreased haemoglobin, haematocrit and RBC compared with other groups of cats. The data obtained underline the importance of considering retroviral infections in the presence of a broad spectrum of risk factors and laboratory anomalies.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46008444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seoung-Woo Lee, Su-Min Baek, Young-Jin Lee, Jin-Kyu Park
Abstract 70-weeks-old female C57BL6 senescence marker protein 30 knock out mice exhibited anorexia, lethargy and enlarged abdomen because of an intraperitoneal mass. On necropsy, the mouse revealed a large brown-whitish mass located on the mesentery. The mass also exhibited systemic metastasis and spread over in various organs. On microscopic findings, the neoplastic masses were mainly composed of neoplastic round cells characterized by severe anisokaryosis, narrow cytoplasm, round nuclei, prominent nucleoli, and numerous mitotic figures (13-15 in a 400X field). Consequently, the present case was diagnosed as a metastatic lymphoma arising from a mesenteric lymph node, the tumor spread to other organs such as the intestine, kidney and thoracic cavity. According to previous studies, SMP30 plays an important role in inhibiting cancer in both human and mouse. Taken together, it seems that the present case can be used as a valuable asset for evaluating the potential risks of SMP30 depletion in developing lymphoma.
{"title":"Spontaneous Lymphoma in a SMP30 Knock-Out C57BL/6 Mouse","authors":"Seoung-Woo Lee, Su-Min Baek, Young-Jin Lee, Jin-Kyu Park","doi":"10.2478/acve-2022-0040","DOIUrl":"https://doi.org/10.2478/acve-2022-0040","url":null,"abstract":"Abstract 70-weeks-old female C57BL6 senescence marker protein 30 knock out mice exhibited anorexia, lethargy and enlarged abdomen because of an intraperitoneal mass. On necropsy, the mouse revealed a large brown-whitish mass located on the mesentery. The mass also exhibited systemic metastasis and spread over in various organs. On microscopic findings, the neoplastic masses were mainly composed of neoplastic round cells characterized by severe anisokaryosis, narrow cytoplasm, round nuclei, prominent nucleoli, and numerous mitotic figures (13-15 in a 400X field). Consequently, the present case was diagnosed as a metastatic lymphoma arising from a mesenteric lymph node, the tumor spread to other organs such as the intestine, kidney and thoracic cavity. According to previous studies, SMP30 plays an important role in inhibiting cancer in both human and mouse. Taken together, it seems that the present case can be used as a valuable asset for evaluating the potential risks of SMP30 depletion in developing lymphoma.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47498129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Risto Dučić, B. Prokic, M. Hadži-Milić, N. Krstić, Vera Todorović, Nikola Radmanović, Maja Đorđević, M. Daković, Farah Hamzagić, S. Pajić
Abstract The period of bone healing after trauma goes through certain physiological processes. In situations where a bone fracture occurs, our intention is to surgically position in an appropriate morpho-anatomical position. For osteosynthesis we used vipla wire and micro plates with corresponding screws. The aim of our research was to evaluate the optimal time for the removal of the osteosynthetic material, fracture site biomechanical stability, and measurement of the callus thickness. For this research ten 4 months old rabbits with right leg femur fracture were used. Osteosynthesis was done with titanium micro plates, corresponding screws and vipla wire. Experimental animals were observed through the post-operative period at the first and seventh postoperative day and there on at 2, 4, 6, 9, 12 weeks. Further additional fixation was needed in one of the rabbits in the second week. The postoperative period provided us information about the optimal moment for the removal of titanium micro plates. It showed that over a period of six weeks, the bone had almost all normal biomechanical properties. Significantly greater strength and rigidity of the recovered femur were obtained after 12 weeks. Micro plates that were removed after 4, 6 or 9 weeks, did not need furhter fixation and therefore fixation was not applied, compared to those placed during the 12 weeks. X Ray evaluation provided us with follow up results of fracture healing. The results suggest that it is acceptable to remove titanium micro plates after a period of healing, that is, after the formation of the callus and bridging of the fracture gap and diastasis with newly formed bone.
{"title":"Correlation of Bone Strength in an Animal Model (Rabbit) After Fracture and During the Period of Fixation with a Titanium Micro Plate","authors":"Risto Dučić, B. Prokic, M. Hadži-Milić, N. Krstić, Vera Todorović, Nikola Radmanović, Maja Đorđević, M. Daković, Farah Hamzagić, S. Pajić","doi":"10.2478/acve-2022-0036","DOIUrl":"https://doi.org/10.2478/acve-2022-0036","url":null,"abstract":"Abstract The period of bone healing after trauma goes through certain physiological processes. In situations where a bone fracture occurs, our intention is to surgically position in an appropriate morpho-anatomical position. For osteosynthesis we used vipla wire and micro plates with corresponding screws. The aim of our research was to evaluate the optimal time for the removal of the osteosynthetic material, fracture site biomechanical stability, and measurement of the callus thickness. For this research ten 4 months old rabbits with right leg femur fracture were used. Osteosynthesis was done with titanium micro plates, corresponding screws and vipla wire. Experimental animals were observed through the post-operative period at the first and seventh postoperative day and there on at 2, 4, 6, 9, 12 weeks. Further additional fixation was needed in one of the rabbits in the second week. The postoperative period provided us information about the optimal moment for the removal of titanium micro plates. It showed that over a period of six weeks, the bone had almost all normal biomechanical properties. Significantly greater strength and rigidity of the recovered femur were obtained after 12 weeks. Micro plates that were removed after 4, 6 or 9 weeks, did not need furhter fixation and therefore fixation was not applied, compared to those placed during the 12 weeks. X Ray evaluation provided us with follow up results of fracture healing. The results suggest that it is acceptable to remove titanium micro plates after a period of healing, that is, after the formation of the callus and bridging of the fracture gap and diastasis with newly formed bone.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49019019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Enrico Stefanizzi, A. Valenčáková, Katarína Schmiesterová, M. Figurová, Ľ. Horňáková
Abstract Doppler ultrasonography is method suitable for observing and measuring the blood supply of many organs. Its principle is the change in the frequency of wave blood that moves with respect to the source of the wave (probe). In a testicular examination, the use of Doppler can also be very useful for detecting abnormalities or pathological conditions. The aim is just to characterize the flow in arteria testicularis in clinically healthy dogs aged 1-5 years. Twelve dogs were included in the study, of which 6 were rough collies weighing approximately 23 kg and 6 were standard dachshunds weighing approximately 9 kg. The pulsatility index (PI) and resistance index (RI) of a.testicularis, as well as the dimensions of the testicles - height and depth, were assessed by ultrasonographic examination using the ALOKA ProSound Alpha 6 ultrasonographic device (Hitachi-Aloka, Japan). The statistical evaluation of the results shows that the differences between the averages of both measurements of PI and RI indices are highly significant, so the weight of the individual had a high effect on the measured values of PI and RI indices. The depth of the testicles was also directly related to the increasing values of PI and RI indices and to the weight of the dogs, in contrast to their height, which did not show any statistical significance in this respect. The measured values and findings may in the future also serve as a basis for determining reference values in testicular ultrasonography according to the weight of dogs, which may be beneficial in clinical status assessment, diagnosis of various pathological conditions, or predicting spermatogenesis and fertility of the individuals.
{"title":"The Use of Doppler Ultrasonography in the Examination of Testicles in Dogs","authors":"Enrico Stefanizzi, A. Valenčáková, Katarína Schmiesterová, M. Figurová, Ľ. Horňáková","doi":"10.2478/acve-2022-0037","DOIUrl":"https://doi.org/10.2478/acve-2022-0037","url":null,"abstract":"Abstract Doppler ultrasonography is method suitable for observing and measuring the blood supply of many organs. Its principle is the change in the frequency of wave blood that moves with respect to the source of the wave (probe). In a testicular examination, the use of Doppler can also be very useful for detecting abnormalities or pathological conditions. The aim is just to characterize the flow in arteria testicularis in clinically healthy dogs aged 1-5 years. Twelve dogs were included in the study, of which 6 were rough collies weighing approximately 23 kg and 6 were standard dachshunds weighing approximately 9 kg. The pulsatility index (PI) and resistance index (RI) of a.testicularis, as well as the dimensions of the testicles - height and depth, were assessed by ultrasonographic examination using the ALOKA ProSound Alpha 6 ultrasonographic device (Hitachi-Aloka, Japan). The statistical evaluation of the results shows that the differences between the averages of both measurements of PI and RI indices are highly significant, so the weight of the individual had a high effect on the measured values of PI and RI indices. The depth of the testicles was also directly related to the increasing values of PI and RI indices and to the weight of the dogs, in contrast to their height, which did not show any statistical significance in this respect. The measured values and findings may in the future also serve as a basis for determining reference values in testicular ultrasonography according to the weight of dogs, which may be beneficial in clinical status assessment, diagnosis of various pathological conditions, or predicting spermatogenesis and fertility of the individuals.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44869985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sveta Arsić, I. Vujanac, J. Bojkovski, D. Kirovski, Sreten Nedić, R. Prodanović
Abstract This study aimed to evaluate the effects of two different treatment options for the correction of left displaced abomasum (LDA) on milk yield, hematological, electrolyte parameters, lactate and cortisol concentrations in primiparous cows. Twenty four Holstein cows were randomly assigned into three groups: cows treated with one-step laparoscopic abomasopexy (LPS, n=8), cows treated by left paralumbar omentopexy (LPT, n=8) and healthy cows (CON, n=8), matched by parity and days in milk. Blood samples were collected before (D0) and after (D0’) surgery, and 1 (D1), 3 (D3), 10 (D3) and 30 (D30) days following surgery. LPS and LPT cows at D0 as well as LPT cows at 30 d following surgery had lower milk yield than CON cows (P<0.05), while the service period was higher in LPT than in CON (P<0.05). WBC was lower at D0 as well as Hb and Ht at D0 and D0’ in CON group than those of LPS and LPT (P<0.05). Hyponatremia, hypochloremia and hypokalemia at D0 and D0’ were observed in LPS and LPT. In addition, LPT cows had lower Na and Cl at D1 and D3 and lower K at D1 than CON (P<0.05). Impaired hydration in LPS and LPT cows was accompanied by higher concentrations of lactate at D0, D0’, D1 and D3 (P<0.01) and cortisol at D0 and D0’ (P<0.01) compared with CON group, while LPT had higher cortisol at D0’ than LPS (P<0.05). These results indicated that LPS has the potential to improve the convalescence period of LDA in primiparous cows.
{"title":"Milk Yield, Hematological and Electrolyte Parameters in Primiparous Dairy Cows After Laparotomic Omentopexy and One-Step Laparoscopic Abomasopexy Treatments of Left Displaced Abomasum","authors":"Sveta Arsić, I. Vujanac, J. Bojkovski, D. Kirovski, Sreten Nedić, R. Prodanović","doi":"10.2478/acve-2022-0038","DOIUrl":"https://doi.org/10.2478/acve-2022-0038","url":null,"abstract":"Abstract This study aimed to evaluate the effects of two different treatment options for the correction of left displaced abomasum (LDA) on milk yield, hematological, electrolyte parameters, lactate and cortisol concentrations in primiparous cows. Twenty four Holstein cows were randomly assigned into three groups: cows treated with one-step laparoscopic abomasopexy (LPS, n=8), cows treated by left paralumbar omentopexy (LPT, n=8) and healthy cows (CON, n=8), matched by parity and days in milk. Blood samples were collected before (D0) and after (D0’) surgery, and 1 (D1), 3 (D3), 10 (D3) and 30 (D30) days following surgery. LPS and LPT cows at D0 as well as LPT cows at 30 d following surgery had lower milk yield than CON cows (P<0.05), while the service period was higher in LPT than in CON (P<0.05). WBC was lower at D0 as well as Hb and Ht at D0 and D0’ in CON group than those of LPS and LPT (P<0.05). Hyponatremia, hypochloremia and hypokalemia at D0 and D0’ were observed in LPS and LPT. In addition, LPT cows had lower Na and Cl at D1 and D3 and lower K at D1 than CON (P<0.05). Impaired hydration in LPS and LPT cows was accompanied by higher concentrations of lactate at D0, D0’, D1 and D3 (P<0.01) and cortisol at D0 and D0’ (P<0.01) compared with CON group, while LPT had higher cortisol at D0’ than LPS (P<0.05). These results indicated that LPS has the potential to improve the convalescence period of LDA in primiparous cows.","PeriodicalId":55559,"journal":{"name":"Acta Veterinaria-Beograd","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43038620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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