Nara Sarmento Macedo Signorelli MSc, DDS, Sávio Gabriel Silva Rende DDS, Eugenio Luigi Iorio PhD, Danilo Cassiano Ferraz MSc, DDS, Luiz Renato Paranhos PhD, Camilla Christian Gomes Moura PhD
Oxidative stress (OS) is a ubiquitous process for protecting against microorganisms' challenges. This review maps the most used methods for obtaining samples and analysing reactive oxygen species levels in apical periodontitis, following the PRISMA Extension for Scoping Reviews and is registered in Open Science Framework ([https://doi.org/10.17605/OSF.IO/D5U76]). A systematic search was conducted in electronic databases MEDLINE (PubMed), Embase, Scopus, Web of Science, LILACS, SciElo, OATD and DANS up to 17 July 2023. A total of 18 studies were included, with periapical tissue being the most common sample. Twenty-eight different oxidative stress markers were identified, with inducible nitric oxide synthase being the most prevalent. The use of diverse biomarkers for oxidative stress assessment lacks specificity in identifying particular OS species for evaluating apical periodontitis and potential systemic effects. Studies are necessary to compare results obtained from less invasive methods (such as saliva and crevicular fluid) with those from periapical lesion samples.
氧化应激(OS)是抵御微生物挑战的一个无处不在的过程。本综述按照《范围界定综述的 PRISMA 扩展》(PRISMA Extension for Scoping Reviews)的要求,介绍了牙根尖周炎中获取样本和分析活性氧水平的最常用方法,并在开放科学框架([https://doi.org/10.17605/OSF.IO/D5U76])中进行了注册。截至 2023 年 7 月 17 日,在 MEDLINE (PubMed)、Embase、Scopus、Web of Science、LILACS、SciElo、OATD 和 DANS 等电子数据库中进行了系统检索。共纳入 18 项研究,其中根尖周组织是最常见的样本。研究发现了 28 种不同的氧化应激标记物,其中以诱导型一氧化氮合酶最为普遍。使用不同的生物标记物评估氧化应激缺乏特异性,无法确定特定的 OS 种类来评估根尖牙周炎和潜在的全身影响。有必要进行研究,将侵入性较小的方法(如唾液和缝隙液)与根尖周病变样本的结果进行比较。
{"title":"Identification of oxidative stress biomarkers in apical periodontitis: A scoping review with bibliometric analysis","authors":"Nara Sarmento Macedo Signorelli MSc, DDS, Sávio Gabriel Silva Rende DDS, Eugenio Luigi Iorio PhD, Danilo Cassiano Ferraz MSc, DDS, Luiz Renato Paranhos PhD, Camilla Christian Gomes Moura PhD","doi":"10.1111/aej.12888","DOIUrl":"10.1111/aej.12888","url":null,"abstract":"<p>Oxidative stress (OS) is a ubiquitous process for protecting against microorganisms' challenges. This review maps the most used methods for obtaining samples and analysing reactive oxygen species levels in apical periodontitis, following the PRISMA Extension for Scoping Reviews and is registered in Open Science Framework ([https://doi.org/10.17605/OSF.IO/D5U76]). A systematic search was conducted in electronic databases MEDLINE (PubMed), Embase, Scopus, Web of Science, LILACS, SciElo, OATD and DANS up to 17 July 2023. A total of 18 studies were included, with periapical tissue being the most common sample. Twenty-eight different oxidative stress markers were identified, with inducible nitric oxide synthase being the most prevalent. The use of diverse biomarkers for oxidative stress assessment lacks specificity in identifying particular OS species for evaluating apical periodontitis and potential systemic effects. Studies are necessary to compare results obtained from less invasive methods (such as saliva and crevicular fluid) with those from periapical lesion samples.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"742-760"},"PeriodicalIF":1.3,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142251583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This retrospective analysis reviewed the demographics of patients sustaining dental avulsion injuries at a tertiary dental hospital in Sydney, Australia. Data were extracted from dental records of patients who presented with avulsed permanent anterior teeth and were treated between 1 January 2001 and 30 June 2021. Demographic, clinical and radiographic data from 91 patients with 117 avulsed permanent anterior teeth were available for analysis. The median age of the patients was 12 years (IQR 9.0–17.0). Males accounted for 68.4% of avulsion injuries. Non-organised sports were the most common cause of injury (42.7%). Maxillary central incisors were the most frequently avulsed tooth (83.3%). Peak prevalence of injuries occurred on the weekend. The findings from this study may reflect regional factors such as climate and participation in sport. Anticipatory guidance should be provided to patients at elevated risk of dental avulsion.
{"title":"Permanent tooth avulsions: A retrospective analysis of the demographics and aetiology of cases at a tertiary hospital in Sydney, Australia","authors":"Julia Bradshaw BDS, BScD(Hons), DClinDent(Paed Dent), MRACDS (Paed Dent), Bill Kahler DClinDent (Endo), PhD, Shanika Nanayakkara MBBS, PhD, Neeta Prabhu BDS, MDS (Paed Dent), MPhil, MRACDS (Paed Dent), FRACDS, FICD","doi":"10.1111/aej.12891","DOIUrl":"10.1111/aej.12891","url":null,"abstract":"<p>This retrospective analysis reviewed the demographics of patients sustaining dental avulsion injuries at a tertiary dental hospital in Sydney, Australia. Data were extracted from dental records of patients who presented with avulsed permanent anterior teeth and were treated between 1 January 2001 and 30 June 2021. Demographic, clinical and radiographic data from 91 patients with 117 avulsed permanent anterior teeth were available for analysis. The median age of the patients was 12 years (IQR 9.0–17.0). Males accounted for 68.4% of avulsion injuries. Non-organised sports were the most common cause of injury (42.7%). Maxillary central incisors were the most frequently avulsed tooth (83.3%). Peak prevalence of injuries occurred on the weekend. The findings from this study may reflect regional factors such as climate and participation in sport. Anticipatory guidance should be provided to patients at elevated risk of dental avulsion.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"640-648"},"PeriodicalIF":1.3,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aej.12891","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142251586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Jenisha Post Graduate, K. G. Geeth Deepika MDS, I. Anand Sherwood MDS, PhD, A. Azhagu Abirami MDS
This study aimed to clinically evaluate the survivability of surgically extruded teeth, to assess the success rate and predictable factors pertaining to alterations in both hard and soft tissues adjacent to extruded teeth. A total of 46 complicated crown-root fractured maxillary central and lateral incisors with >75% tooth structure loss relatively due to trauma were included. Preoperative clinical and radiographic variables were recorded. Single operator performed endodontic treatment before surgical extrusion. Patients were recalled for review at 6, 12, 18, 24 and 36 months. Survivability was assessed using Kaplan–Meier survival analysis. With a mean follow-up of 2.8 years, survival rate was 95.7%. Mobility observed in only two cases. The crown-root ratio was favourable (1:2) in all the cases except for five teeth. Only two teeth displayed non-progressive root resorption. Survival of surgically extruded teeth relies on significant predictors like favourability of CRR and implementation of atraumatic extrusion.
{"title":"Survival evaluation of surgical extrusion for the management of complicated crown-root fractured maxillary incisors: A clinical study","authors":"J. Jenisha Post Graduate, K. G. Geeth Deepika MDS, I. Anand Sherwood MDS, PhD, A. Azhagu Abirami MDS","doi":"10.1111/aej.12889","DOIUrl":"10.1111/aej.12889","url":null,"abstract":"<p>This study aimed to clinically evaluate the survivability of surgically extruded teeth, to assess the success rate and predictable factors pertaining to alterations in both hard and soft tissues adjacent to extruded teeth. A total of 46 complicated crown-root fractured maxillary central and lateral incisors with >75% tooth structure loss relatively due to trauma were included. Preoperative clinical and radiographic variables were recorded. Single operator performed endodontic treatment before surgical extrusion. Patients were recalled for review at 6, 12, 18, 24 and 36 months. Survivability was assessed using Kaplan–Meier survival analysis. With a mean follow-up of 2.8 years, survival rate was 95.7%. Mobility observed in only two cases. The crown-root ratio was favourable (1:2) in all the cases except for five teeth. Only two teeth displayed non-progressive root resorption. Survival of surgically extruded teeth relies on significant predictors like favourability of CRR and implementation of atraumatic extrusion.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"629-639"},"PeriodicalIF":1.3,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142194329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to evaluate and compare the shaping abilities and minimum dentin thickness of minimally invasive rotary instruments via micro-computed tomography. Twelve 3D-printed C-shaped canal models from a mandibular molar were divided into two groups, and root canals were prepared with either XP-endo Rise (XR) or TruNatomy (TN) systems. Pre- and post-preparation evaluations included canal volume, prepared area and minimum dentin thickness. No significant differences were found in canal volume change (XR: 22.66 ± 4.28%, TN: 23.02 ± 5.10%), prepared canal area (XR: 31.86 ± 10.72%, TN: 30.26 ± 11.59%) and minimum dentin thickness (XR: 0.30 ± 0.05 mm, TN: 0.28 ± 0.05 mm) between groups (p > 0.05). The canal volume change in the middle third was significantly higher than that in the coronal and apical thirds (p < 0.05) in both groups. In conclusion, XR demonstrated comparable shaping abilities and minimum dentin thickness to TN in preparing 3D-printed C-shaped canals.
{"title":"Micro-computed tomography evaluation of minimally invasive root canal preparation in 3D-printed C-shaped canal","authors":"Nutcha Supavititpattana DDS, Siriwan Suebnukarn DDS, PhD, Panupat Phumpatrakom DDS, MSc, FRCDS, Kamon Budsaba PhD","doi":"10.1111/aej.12890","DOIUrl":"10.1111/aej.12890","url":null,"abstract":"<p>This study aimed to evaluate and compare the shaping abilities and minimum dentin thickness of minimally invasive rotary instruments via micro-computed tomography. Twelve 3D-printed C-shaped canal models from a mandibular molar were divided into two groups, and root canals were prepared with either XP-endo Rise (XR) or TruNatomy (TN) systems. Pre- and post-preparation evaluations included canal volume, prepared area and minimum dentin thickness. No significant differences were found in canal volume change (XR: 22.66 ± 4.28%, TN: 23.02 ± 5.10%), prepared canal area (XR: 31.86 ± 10.72%, TN: 30.26 ± 11.59%) and minimum dentin thickness (XR: 0.30 ± 0.05 mm, TN: 0.28 ± 0.05 mm) between groups (<i>p</i> > 0.05). The canal volume change in the middle third was significantly higher than that in the coronal and apical thirds (<i>p</i> < 0.05) in both groups. In conclusion, XR demonstrated comparable shaping abilities and minimum dentin thickness to TN in preparing 3D-printed C-shaped canals.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"621-628"},"PeriodicalIF":1.3,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142194328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Reham Hassan, Maha Nasr, Hams H. Abdelrahman, Mohamed G. Hassan
The purpose of this study was to analyse the social media activity related to endodontic research over the last 10-years. All research articles published in endodontic journals listed in Scopus (Sc) published in 2012 and 2018 were included in our study. The Altmetric Attention Score (AAS), Twitter, and Facebook mentions were obtained for each article. Citation counts were extracted using two citation metrics: Google Scholar (GS) and Sc. Correlations between the AAS, the number of social media mentions, and citations were analysed using Spearman's rank order correlation coefficient. A multivariable Poisson log-linear regression analysis shows that papers mentioned on social media gain about 35% more citations in GS and 31% more citations in Sc. The academic citations per article on GS and Sc were positively correlated with the AAS. Our data suggest an increasing positive correlation between social media mentions and article citations over the years.
{"title":"Sharing endodontic research on social media platforms: Is it effective? A 10-year timeline analysis","authors":"Reham Hassan, Maha Nasr, Hams H. Abdelrahman, Mohamed G. Hassan","doi":"10.1111/aej.12887","DOIUrl":"10.1111/aej.12887","url":null,"abstract":"<p>The purpose of this study was to analyse the social media activity related to endodontic research over the last 10-years. All research articles published in endodontic journals listed in Scopus (Sc) published in 2012 and 2018 were included in our study. The Altmetric Attention Score (AAS), Twitter, and Facebook mentions were obtained for each article. Citation counts were extracted using two citation metrics: Google Scholar (GS) and Sc. Correlations between the AAS, the number of social media mentions, and citations were analysed using Spearman's rank order correlation coefficient. A multivariable Poisson log-linear regression analysis shows that papers mentioned on social media gain about 35% more citations in GS and 31% more citations in Sc. The academic citations per article on GS and Sc were positively correlated with the AAS. Our data suggest an increasing positive correlation between social media mentions and article citations over the years.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"612-620"},"PeriodicalIF":1.3,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142134589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan Domingo Saavedra Felipe DDS, Virginia Andrea Watson Molina DDS, MSc, Anabell Bologna Escalona DDS, CDT, Rafael Laplana Martínez DDS, Aitzol Areso DDS, MSc, Venkateshbabu Nagendrababu BDS, MDS, FDS RCPS (Glasg), PhD, Francesc Abella Sans DDS, PhD
Managing teeth with open apices can be a challenging scenario for clinicians. This case report describes the 24-year follow-up of a maxillary central incisor that failed to develop after a traumatic injury resulting in a wide-open apex. A 10-year-old girl presented complaining of discomfort in her upper teeth. Tooth #9 had received a traumatic blow several years before and was discoloured with a resin composite restoration on the mesio-incisal edge. The tooth was painful to percussion and palpation. An inadequate root canal filling and incomplete root formation were observed on the initial periapical radiograph. Root canal retreatment was initiated, and the canal filled entirely with mineral trioxide aggregate (MTA) because of the extremely wide canal and open apex. The patient was asymptomatic at the 24-year follow-up with the cone beam computed tomography and periapical radiographs demonstrating the stability of the MTA.
对于临床医生来说,处理牙尖开放的牙齿是一种具有挑战性的情况。本病例报告描述了对一颗上颌中切牙 24 年的随访情况,这颗上颌中切牙在一次外伤后未能发育,导致牙尖开放过大。一名 10 岁女孩主诉上牙不适。她的 9 号牙齿在几年前受到过一次外伤,中切缘的树脂复合修复体已经变色。叩诊和触诊时牙齿疼痛。在最初的根尖周炎 X 光片上可以看到根管充填不充分,牙根形成不完整。由于根管极宽且根尖开放,因此开始了根管再治疗,并用三氧化物矿物质骨料(MTA)完全填充了根管。在 24 年的随访中,患者没有任何症状,锥形束计算机断层扫描和根尖周X光片显示 MTA 很稳定。
{"title":"Successful retreatment of a maxillary central incisor with a wide-open apex using MTA to fill the entire canal: 24-year follow-up","authors":"Juan Domingo Saavedra Felipe DDS, Virginia Andrea Watson Molina DDS, MSc, Anabell Bologna Escalona DDS, CDT, Rafael Laplana Martínez DDS, Aitzol Areso DDS, MSc, Venkateshbabu Nagendrababu BDS, MDS, FDS RCPS (Glasg), PhD, Francesc Abella Sans DDS, PhD","doi":"10.1111/aej.12885","DOIUrl":"10.1111/aej.12885","url":null,"abstract":"<p>Managing teeth with open apices can be a challenging scenario for clinicians. This case report describes the 24-year follow-up of a maxillary central incisor that failed to develop after a traumatic injury resulting in a wide-open apex. A 10-year-old girl presented complaining of discomfort in her upper teeth. Tooth #9 had received a traumatic blow several years before and was discoloured with a resin composite restoration on the mesio-incisal edge. The tooth was painful to percussion and palpation. An inadequate root canal filling and incomplete root formation were observed on the initial periapical radiograph. Root canal retreatment was initiated, and the canal filled entirely with mineral trioxide aggregate (MTA) because of the extremely wide canal and open apex. The patient was asymptomatic at the 24-year follow-up with the cone beam computed tomography and periapical radiographs demonstrating the stability of the MTA.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"700-708"},"PeriodicalIF":1.3,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636065/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lucas Moreira Maia DDS, MSc, PhD, Gil Moreira Júnior DDS, MSc, PhD, Nelson Renato França Alves Silva DDS, MSc, PhD, Vinícius de Carvalho Machado DDS, Larissa Fassarela Marquiore DDS, Emmanuel João Nogueira Leal Silva DDS, MSc, PhD, Antônio Paulino Ribeiro Sobrinho DDS, MSc, PhD, Warley Luciano Fonseca Tavares DDS, MSc, PhD
Endodontic microsurgery guided by navigation systems represents a precise and minimally invasive approach for retreatment of apical periodontitis following failed conventional endodontic therapy. Accurate localization and minimal access to the root apex are paramount for successful outcomes and preservation of anatomical structures. Workflow considerations, such as three-dimensional (3D) virtual planning and endodontic guidance represent new crucial aspects for addressing complex cases. This clinical case report presents the successful reintervention of a complex case using a Zekrya bur, operative microscopy, cone beam computed tomography and biocompatible materials. After a 48-month follow-up, complete healing of the treated area was observed. This minimally invasive technique, employing a simple instrument widely available globally, underscores the potential for efficient and predictable outcomes in complex endodontic microsurgical retreatment.
{"title":"Minimally invasive guided endodontic microsurgery with Zekrya bur: A 48-month follow-up case report","authors":"Lucas Moreira Maia DDS, MSc, PhD, Gil Moreira Júnior DDS, MSc, PhD, Nelson Renato França Alves Silva DDS, MSc, PhD, Vinícius de Carvalho Machado DDS, Larissa Fassarela Marquiore DDS, Emmanuel João Nogueira Leal Silva DDS, MSc, PhD, Antônio Paulino Ribeiro Sobrinho DDS, MSc, PhD, Warley Luciano Fonseca Tavares DDS, MSc, PhD","doi":"10.1111/aej.12884","DOIUrl":"10.1111/aej.12884","url":null,"abstract":"<p>Endodontic microsurgery guided by navigation systems represents a precise and minimally invasive approach for retreatment of apical periodontitis following failed conventional endodontic therapy. Accurate localization and minimal access to the root apex are paramount for successful outcomes and preservation of anatomical structures. Workflow considerations, such as three-dimensional (3D) virtual planning and endodontic guidance represent new crucial aspects for addressing complex cases. This clinical case report presents the successful reintervention of a complex case using a Zekrya bur, operative microscopy, cone beam computed tomography and biocompatible materials. After a 48-month follow-up, complete healing of the treated area was observed. This minimally invasive technique, employing a simple instrument widely available globally, underscores the potential for efficient and predictable outcomes in complex endodontic microsurgical retreatment.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"687-692"},"PeriodicalIF":1.3,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142005938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to evaluate the antibiofilm activity of different concentrations of silver nanoparticles (AgNPs) in combination with calcium hydroxide [Ca(OH)2] against Enterococcus faecalis biofilm. On an E. faecalis biofilm on dentin discs, the following medicaments were applied for 7 days (n = 13/group): 0.005% AgNPs+Ca(OH)2, 0.01% AgNPs+Ca(OH)2, 0.02% AgNPs + Ca(OH)2, Ca(OH)2 and saline/control. Specimens were stained with LIVE/DEAD® BacLight™ dye and analysed with confocal laser scanning microscopy. Proportion of dead bacteria was calculated and analysed. There was a significant reduction in E. faecalis biofilm in all medicament groups (43.5%, 49.1%, 69.1%, 48.7%) respectively, compared with control group (2.54%) (p < 0.001). The 0.02% AgNPs + Ca(OH)2 group demonstrated the most significantly superior antibiofilm effect, with no significant difference between remaining groups. In conclusion, combining 0.02% AgNPs enhanced the antibiofilm effect of Ca(OH)2 on E. faecalis biofilm compared with lower AgNPs concentrations.
{"title":"Antibiofilm effect of different concentrations of silver nanoparticles combined with calcium hydroxide against Enterococcus faecalis biofilm: An ex vivo study","authors":"Sumaya Basudan BDS, MSc, SBE, Alanoud Alqahtani BDS, SBE, Fatemah Alrwais BDS, Reem Almeaither BDS, SBE, Sayed Auda PhD, Hanan A. Balto BDS, MSc","doi":"10.1111/aej.12882","DOIUrl":"10.1111/aej.12882","url":null,"abstract":"<p>This study aimed to evaluate the antibiofilm activity of different concentrations of silver nanoparticles (AgNPs) in combination with calcium hydroxide [Ca(OH)<sub>2</sub>] against <i>Enterococcus faecalis</i> biofilm. On an <i>E. faecalis</i> biofilm on dentin discs, the following medicaments were applied for 7 days (<i>n</i> = 13/group): 0.005% AgNPs+Ca(OH)<sub>2</sub>, 0.01% AgNPs+Ca(OH)<sub>2</sub>, 0.02% AgNPs + Ca(OH)<sub>2</sub>, Ca(OH)<sub>2</sub> and saline/control. Specimens were stained with LIVE/DEAD® <i>Bac</i>Light™ dye and analysed with confocal laser scanning microscopy. Proportion of dead bacteria was calculated and analysed. There was a significant reduction in <i>E. faecalis</i> biofilm in all medicament groups (43.5%, 49.1%, 69.1%, 48.7%) respectively, compared with control group (2.54%) (<i>p</i> < 0.001). The 0.02% AgNPs + Ca(OH)<sub>2</sub> group demonstrated the most significantly superior antibiofilm effect, with no significant difference between remaining groups. In conclusion, combining 0.02% AgNPs enhanced the antibiofilm effect of Ca(OH)<sub>2</sub> on <i>E. faecalis</i> biofilm compared with lower AgNPs concentrations.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"604-611"},"PeriodicalIF":1.3,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141977338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sukainah A. Aldawood BDS, Omar Omar BDS, PhD, Dana Almohazey BSc, MSc, PhD, Amre R. Atmeh BDS, MSc(Endodontics), PhD
This study evaluated the reliability of sealer penetration measurement methods used with confocal laser scanning microscopy in correlation with the percentage of residual root filling and examined the effect of residual root-filling material on dentine penetrability after retreatment. Extracted teeth were randomly divided into different groups according to the obturation sealer used (n = 6); BioRoot RCS; MTA Fillapex; Bio-C and AH Plus. Root-filling material was removed before the secondary chemo-mechanical preparation and obturation using fluorescein labelled AH Plus. Multiple micro-computed tomography scans were obtained followed by confocal laser scanning microscopy to measure the penetration of the labelled sealer into the dentinal tubules using four different methods. Measuring sealer penetration into radicular dentine using the penetration percentage method was found to be the most reliable. Dentine penetrability during retreatment did not seem to be affected by the type of residual root-filling material.
{"title":"Reliability of sealer penetration measurement methods and assessment of dentine penetrability after endodontic retreatment: A correlation in vitro study","authors":"Sukainah A. Aldawood BDS, Omar Omar BDS, PhD, Dana Almohazey BSc, MSc, PhD, Amre R. Atmeh BDS, MSc(Endodontics), PhD","doi":"10.1111/aej.12881","DOIUrl":"10.1111/aej.12881","url":null,"abstract":"<p>This study evaluated the reliability of sealer penetration measurement methods used with confocal laser scanning microscopy in correlation with the percentage of residual root filling and examined the effect of residual root-filling material on dentine penetrability after retreatment. Extracted teeth were randomly divided into different groups according to the obturation sealer used (<i>n</i> = 6); BioRoot RCS; MTA Fillapex; Bio-C and AH Plus. Root-filling material was removed before the secondary chemo-mechanical preparation and obturation using fluorescein labelled AH Plus. Multiple micro-computed tomography scans were obtained followed by confocal laser scanning microscopy to measure the penetration of the labelled sealer into the dentinal tubules using four different methods. Measuring sealer penetration into radicular dentine using the penetration percentage method was found to be the most reliable. Dentine penetrability during retreatment did not seem to be affected by the type of residual root-filling material.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 3","pages":"595-603"},"PeriodicalIF":1.3,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141891115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"APEC News July 2024: Ongoing process for organisation of APEC2025 Dhaka","authors":"Hyeon-Cheol Kim DDS, PhD","doi":"10.1111/aej.12879","DOIUrl":"10.1111/aej.12879","url":null,"abstract":"","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 2","pages":"188-190"},"PeriodicalIF":1.3,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141774216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}