Bruna Venzke Fischer, Luiz Carlos de Lima Dias-Junior, Mariana Comparotto Minamisako, Cristiane Maria Almeida, Luciano Roberto da Silva, Eduardo Antunes Bortoluzzi, Cleonice da Silveira Teixeira, Lucas da Fonseca Roberti Garcia
This study investigated the effect of the timing of primary endodontic treatment and dosage of radiotherapy on the remaining filling material (RFM) during endodontic reintervention. 60 single-rooted human mandibular premolars were distributed into five groups (n = 12), according to the timing and dosage of radiation (55Gy or 70Gy): NegativeCG-non-irradiated teeth; Endo-pre-RT55/70-obturation before irradiation (55Gy or 70Gy); Endo-post-RT55/70-obturation and reintervention after irradiation (55Gy or 70Gy). Roots were cleaved and analysed under stereomicroscope and Scanning Electron Microscope to quantify (%) the RFM. Experimental groups had a significantly greater amount (p < 0.05) of RFM in the middle and apical thirds than the control group, except for Endo-pre-RT55 in the middle third (p < 0.0001). The apical third had greater amount of RFM (p < 0.05). Radiation therapy, before and after primary endodontic treatment, increased the amount of RFM, regardless of the dose delivered. When necessary, reintervention preferably must be performed before radiation therapy.
{"title":"Effect of the timing of primary endodontic treatment and dosage of radiation therapy on the filling material removal","authors":"Bruna Venzke Fischer, Luiz Carlos de Lima Dias-Junior, Mariana Comparotto Minamisako, Cristiane Maria Almeida, Luciano Roberto da Silva, Eduardo Antunes Bortoluzzi, Cleonice da Silveira Teixeira, Lucas da Fonseca Roberti Garcia","doi":"10.1111/aej.12846","DOIUrl":"10.1111/aej.12846","url":null,"abstract":"<p>This study investigated the effect of the timing of primary endodontic treatment and dosage of radiotherapy on the remaining filling material (RFM) during endodontic reintervention. 60 single-rooted human mandibular premolars were distributed into five groups (<i>n</i> = 12), according to the timing and dosage of radiation (55Gy or 70Gy): NegativeCG-non-irradiated teeth; Endo-pre-RT<sub>55/70</sub>-obturation before irradiation (55Gy or 70Gy); Endo-post-RT<sub>55/70</sub>-obturation and reintervention after irradiation (55Gy or 70Gy). Roots were cleaved and analysed under stereomicroscope and Scanning Electron Microscope to quantify (%) the RFM. Experimental groups had a significantly greater amount (<i>p</i> < 0.05) of RFM in the middle and apical thirds than the control group, except for Endo-pre-RT<sub>55</sub> in the middle third (<i>p</i> < 0.0001). The apical third had greater amount of RFM (<i>p</i> < 0.05). Radiation therapy, before and after primary endodontic treatment, increased the amount of RFM, regardless of the dose delivered. When necessary, reintervention preferably must be performed before radiation therapy.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140588172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of this systematic review and meta-analysis is to conduct a comparative evaluation of partial and full pulpotomy techniques in cariously exposed teeth with symptoms indicative of symptomatic irreversible pulpitis. Databases such as PubMed, EMBASE, Cochrane, and Web of Science were searched. Studies evaluating and/or comparing clinical and/or radiographic success of partial and full pulpotomy in teeth diagnosed with irreversible pulpitis with a minimum of 12 months follow-up were included. The risk of bias (ROB) tool was used for the assessment of ROB. A meta-analysis was conducted to compare the healing outcome of partial and full pulpotomy. Three studies fulfilled the inclusion criteria, there was a low risk of bias in each of the five domains. Full pulpotomy had a higher success rate than partial pulpotomy, according to meta-analysis, but the difference was not statistically significant.
本系统综述和荟萃分析的目的是对具有症状性不可逆牙髓炎迹象的龋坏暴露牙的部分和全部牙髓切除术进行比较评估。本文检索了 PubMed、EMBASE、Cochrane 和 Web of Science 等数据库。纳入的研究评估和/或比较了对确诊为不可逆性牙髓炎的牙齿进行部分和全部牙髓切断术的临床和/或放射学成功率,随访时间至少为 12 个月。偏倚风险(ROB)工具用于评估 ROB。为了比较部分和全部牙髓切断术的疗效,我们进行了一项荟萃分析。有三项研究符合纳入标准,在五个方面的偏倚风险均较低。根据荟萃分析结果,全髓核切除术的成功率高于部分髓核切除术,但差异在统计学上并不显著。
{"title":"Comparative evaluation of full and partial pulpotomy in permanent teeth with irreversible pulpitis: A systematic review and meta-analysis","authors":"Vishal Kumar MDS, Amrita Chawla MDS, Harsh Priya MDS, Akshat Sachdeva MDS, Sidhartha Sharma MDS, Vijay Kumar MDS, Ajay Logani MDS","doi":"10.1111/aej.12844","DOIUrl":"10.1111/aej.12844","url":null,"abstract":"<p>The purpose of this systematic review and meta-analysis is to conduct a comparative evaluation of partial and full pulpotomy techniques in cariously exposed teeth with symptoms indicative of symptomatic irreversible pulpitis. Databases such as PubMed, EMBASE, Cochrane, and Web of Science were searched. Studies evaluating and/or comparing clinical and/or radiographic success of partial and full pulpotomy in teeth diagnosed with irreversible pulpitis with a minimum of 12 months follow-up were included. The risk of bias (ROB) tool was used for the assessment of ROB. A meta-analysis was conducted to compare the healing outcome of partial and full pulpotomy. Three studies fulfilled the inclusion criteria, there was a low risk of bias in each of the five domains. Full pulpotomy had a higher success rate than partial pulpotomy, according to meta-analysis, but the difference was not statistically significant.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140588051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mariam Ahmed Hossam BDS, Alaa Abdelsalam El Baz BDS, MSc, PhD, Sang Won Kwak DDS, MS, PhD, Hyeon-Cheol Kim DDS, MS, PhD, Mohamed Mohsen Abielhassan BDS, MSc, PhD
The aim of this study was to assess the effect of ibuprofen sustained release (SR) oral premedication on the efficacy of buccal infiltration (BI) with intraoperative and postoperative pain after single-visit root canal treatment. Sixty patients diagnosed with symptomatic irreversible pulpitis and apical periodontitis in mandibular molar were divided into two groups. Group SR received ibuprofen SR 800 mg and group PL received placebo capsule 1 h before 3.6 mL articaine BI injection. Pain was recorded using a modified visual analogue scale and postoperatively at intervals 6, 24 and 48 h. Group SR showed a significantly higher anaesthetic success rate (73.3%) compared to group PL (46.7%) (p < 0.05). Intraoperative and postoperative pain was significantly higher in group PL compared to group SR (p < 0.05). Premedication of ibuprofen SR improved the efficacy of primary BI in mandibular molars with symptomatic irreversible pulpitis and decreased postoperative pain at 6 and 48 h.
本研究旨在评估布洛芬缓释剂(SR)口服预处理对单次根管治疗后颊面浸润(BI)术中和术后疼痛疗效的影响。将 60 名被诊断为下颌磨牙无症状不可逆牙髓炎和根尖牙周炎的患者分为两组。SR 组在注射 3.6 毫升青蒿素 BI 前 1 小时服用布洛芬 SR 800 毫克,PL 组服用安慰剂胶囊。使用改良视觉模拟量表记录疼痛情况,并在术后 6、24 和 48 h 间隔时间进行记录。SR 组的麻醉成功率(73.3%)明显高于 PL 组(46.7%)(p
{"title":"The effect of ibuprofen sustained release oral premedication on intraoperative and postoperative pain: A randomised clinical trial","authors":"Mariam Ahmed Hossam BDS, Alaa Abdelsalam El Baz BDS, MSc, PhD, Sang Won Kwak DDS, MS, PhD, Hyeon-Cheol Kim DDS, MS, PhD, Mohamed Mohsen Abielhassan BDS, MSc, PhD","doi":"10.1111/aej.12839","DOIUrl":"10.1111/aej.12839","url":null,"abstract":"<p>The aim of this study was to assess the effect of ibuprofen sustained release (SR) oral premedication on the efficacy of buccal infiltration (BI) with intraoperative and postoperative pain after single-visit root canal treatment. Sixty patients diagnosed with symptomatic irreversible pulpitis and apical periodontitis in mandibular molar were divided into two groups. Group SR received ibuprofen SR 800 mg and group PL received placebo capsule 1 h before 3.6 mL articaine BI injection. Pain was recorded using a modified visual analogue scale and postoperatively at intervals 6, 24 and 48 h. Group SR showed a significantly higher anaesthetic success rate (73.3%) compared to group PL (46.7%) (<i>p</i> < 0.05). Intraoperative and postoperative pain was significantly higher in group PL compared to group SR (<i>p</i> < 0.05). Premedication of ibuprofen SR improved the efficacy of primary BI in mandibular molars with symptomatic irreversible pulpitis and decreased postoperative pain at 6 and 48 h.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140289744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jesús Moreno-Pérez DDS, MSc, David Rubio Flores DDS, MSc, PhD, Carlos Fernández Villares, Venkateshbabu Nagendrababu BDS, MDS, FDS RCPS (Glasg), PhD, Francesc Abella Sans DDS, PhD
The aim of this report is to demonstrate the guided tooth autotransplantation of a palatally impacted maxillary canine into the site of a failed maxillary canine dental implant. A 47-year-old woman visited a dental clinic complaining of loose dental implant in the left maxillary canine site, tooth #11, as well as pain and swelling of the gum around the implant. The clinical examination revealed a mobile implant along with swollen soft tissues with bleeding on probing. A periapical radiograph demonstrated peri-implant marginal bone loss. Cone beam computed tomography sections revealed that tooth #11 was impacted palatally. The implant was removed and replaced with the impacted canine via guided autotransplantation and posterior orthodontic alignment. The patient was recalled at 1, 3, 6, 9, 12, 24 and 48 months after the procedure. During this period, the patient was symptom-free and radiographic examination at 2 years revealed no periapical pathosis or root resorption.
{"title":"Guided tooth autotransplantation of a palatally impacted maxillary canine into the site of a failed maxillary canine implant","authors":"Jesús Moreno-Pérez DDS, MSc, David Rubio Flores DDS, MSc, PhD, Carlos Fernández Villares, Venkateshbabu Nagendrababu BDS, MDS, FDS RCPS (Glasg), PhD, Francesc Abella Sans DDS, PhD","doi":"10.1111/aej.12840","DOIUrl":"10.1111/aej.12840","url":null,"abstract":"<p>The aim of this report is to demonstrate the guided tooth autotransplantation of a palatally impacted maxillary canine into the site of a failed maxillary canine dental implant. A 47-year-old woman visited a dental clinic complaining of loose dental implant in the left maxillary canine site, tooth #11, as well as pain and swelling of the gum around the implant. The clinical examination revealed a mobile implant along with swollen soft tissues with bleeding on probing. A periapical radiograph demonstrated peri-implant marginal bone loss. Cone beam computed tomography sections revealed that tooth #11 was impacted palatally. The implant was removed and replaced with the impacted canine via guided autotransplantation and posterior orthodontic alignment. The patient was recalled at 1, 3, 6, 9, 12, 24 and 48 months after the procedure. During this period, the patient was symptom-free and radiographic examination at 2 years revealed no periapical pathosis or root resorption.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/aej.12840","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140177949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Érika Mayumi Omoto, Anderson Catelan, Paulo Henrique dos Santos, Luciano Tavares Angelo Cintra, Fernanda de Souza e Silva Ramos, Caio César Pavani, André Luiz Fraga Briso, Ticiane Cestari Fagundes
This study aimed to evaluate the interface of universal adhesive (UA) with bulk-fill resin (BF) and dentin, after endodontic irrigation. The pulp chamber of 96 molars was irrigated with chlorhexidine 2% (CHX) or sodium hypochlorite 5.25% (NaOCl) and restored according to three restorative protocols. Microtensile bond strength (μTBS) and nanoleakage tests were performed after 24 h of thermocycling. Kruskal Wallis, Mann–Whitney and Wilcoxon were performed (p < 0.05). Comparing restorative systems, a difference was found only when irrigated with CHX after 24 h. CHX provided higher μTBS than NaOCl after 24 h for two restorative protocols; after thermocycling, NaOCl provided the greatest μTBS than CHX for one group. Decreased μTBS was observed for all restorative systems when CHX was used after thermocycling. NaOCl demonstrated the highest nanoleakage. The composition of UA influenced the immediate adhesion and sealing to BF when CHX was used; ageing has a deteriorating effect on adhesion and sealing for all restorative systems.
{"title":"Adhesion and sealing of different universal adhesive systems associated with bulk-fill resins after using endodontic irrigation solutions: An in vitro study","authors":"Érika Mayumi Omoto, Anderson Catelan, Paulo Henrique dos Santos, Luciano Tavares Angelo Cintra, Fernanda de Souza e Silva Ramos, Caio César Pavani, André Luiz Fraga Briso, Ticiane Cestari Fagundes","doi":"10.1111/aej.12843","DOIUrl":"10.1111/aej.12843","url":null,"abstract":"<p>This study aimed to evaluate the interface of universal adhesive (UA) with bulk-fill resin (BF) and dentin, after endodontic irrigation. The pulp chamber of 96 molars was irrigated with chlorhexidine 2% (CHX) or sodium hypochlorite 5.25% (NaOCl) and restored according to three restorative protocols. Microtensile bond strength (μTBS) and nanoleakage tests were performed after 24 h of thermocycling. Kruskal Wallis, Mann–Whitney and Wilcoxon were performed (<i>p</i> < 0.05). Comparing restorative systems, a difference was found only when irrigated with CHX after 24 h. CHX provided higher μTBS than NaOCl after 24 h for two restorative protocols; after thermocycling, NaOCl provided the greatest μTBS than CHX for one group. Decreased μTBS was observed for all restorative systems when CHX was used after thermocycling. NaOCl demonstrated the highest nanoleakage. The composition of UA influenced the immediate adhesion and sealing to BF when CHX was used; ageing has a deteriorating effect on adhesion and sealing for all restorative systems.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140177946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Michael Arnold Dipl.-Stom., Hany Mohamed Aly Ahmed BDS, HDD (Endo), PhD, FICD, MDTFEd (RCSEd), FPFA, FADI
This report presents the detection, characterisation, instrumentation and filling of complex canal configurations in the mesiobuccal (MB) root of maxillary first molars. Three patients were referred for root canal treatment in first maxillary molars. Medical history, age, sex and clinical findings were recorded. Intraoral periapical radiographs and cone beam computed tomography (CBCT) were used for diagnosis and pre-operative assessment. Using Ahmed et al. (2017) classification system, codes 3MaxM MB3-5-4-2 DB1-2-1 P1 (case 1), 3MaxM MB2-5-3-2 DB1 P1 (case 2) and 3MaxM MB1-4-2-1-2 DB1 P1 (case 3) were identified. Using the dental operating microscope, CBCT (if indicated) and troughing up to 3 mm allowed identification of more than three canals in the MB root of maxillary first molars. The coding system proposed by Ahmed et al. (2017) allows the classification of MB roots with highly complex canal configurations.
{"title":"Detection, characterisation and management of complex root canal configurations in the mesiobuccal roots of maxillary first molars: A case series","authors":"Michael Arnold Dipl.-Stom., Hany Mohamed Aly Ahmed BDS, HDD (Endo), PhD, FICD, MDTFEd (RCSEd), FPFA, FADI","doi":"10.1111/aej.12842","DOIUrl":"10.1111/aej.12842","url":null,"abstract":"<p>This report presents the detection, characterisation, instrumentation and filling of complex canal configurations in the mesiobuccal (MB) root of maxillary first molars. Three patients were referred for root canal treatment in first maxillary molars. Medical history, age, sex and clinical findings were recorded. Intraoral periapical radiographs and cone beam computed tomography (CBCT) were used for diagnosis and pre-operative assessment. Using Ahmed et al. (2017) classification system, codes <sup>3</sup>MaxM MB<sup>3-5-4-2</sup> DB<sup>1-2-1</sup> P<sup>1</sup> (case 1), <sup>3</sup>MaxM MB<sup>2-5-3-2</sup> DB<sup>1</sup> P<sup>1</sup> (case 2) and <sup>3</sup>MaxM MB<sup>1-4-2-1-2</sup> DB<sup>1</sup> P<sup>1</sup> (case 3) were identified. Using the dental operating microscope, CBCT (if indicated) and troughing up to 3 mm allowed identification of more than three canals in the MB root of maxillary first molars. The coding system proposed by Ahmed et al. (2017) allows the classification of MB roots with highly complex canal configurations.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140177947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Kırmızı DDS, PhD, A. Ö. Sehirli PhD, S. Sayiner DVM, K. Orhan DDS, PhD, A. Sebai DDS, PhD, U. Aksoy DDS, PhD
Apical periodontitis is an inflammatory condition resulting from microbial invasion in the root canal system, causing periapical tissue destruction and bone resorption. This study investigated melatonin's effects, known for its antioxidant and anti-inflammatory properties, on experimentally induced apical periodontitis in rats. Three groups of rats were studied: control, apical periodontitis and apical periodontitis with melatonin treatment. Proinflammatory cytokines and enzyme levels in blood serum were measured, and micro-CT analysis assessed bone resorption. Results showed significantly elevated cytokines and enzyme levels in the apical periodontitis group compared to the control. However, in the melatonin-treated group, these levels were significantly reduced (p < 0.01–0.001). Micro-CT analysis indicated decreased periapical resorption cavity volume and surface area with melatonin treatment. This suggests that systemic melatonin administration can mitigate inflammation and reduce bone resorption in experimentally induced apical periodontitis in rats, potentially holding promise for human endodontic disease treatment pending further research.
{"title":"Effects of melatonin against experimentally induced apical periodontitis in rats","authors":"D. Kırmızı DDS, PhD, A. Ö. Sehirli PhD, S. Sayiner DVM, K. Orhan DDS, PhD, A. Sebai DDS, PhD, U. Aksoy DDS, PhD","doi":"10.1111/aej.12841","DOIUrl":"10.1111/aej.12841","url":null,"abstract":"<p>Apical periodontitis is an inflammatory condition resulting from microbial invasion in the root canal system, causing periapical tissue destruction and bone resorption. This study investigated melatonin's effects, known for its antioxidant and anti-inflammatory properties, on experimentally induced apical periodontitis in rats. Three groups of rats were studied: control, apical periodontitis and apical periodontitis with melatonin treatment. Proinflammatory cytokines and enzyme levels in blood serum were measured, and micro-CT analysis assessed bone resorption. Results showed significantly elevated cytokines and enzyme levels in the apical periodontitis group compared to the control. However, in the melatonin-treated group, these levels were significantly reduced (<i>p</i> < 0.01–0.001). Micro-CT analysis indicated decreased periapical resorption cavity volume and surface area with melatonin treatment. This suggests that systemic melatonin administration can mitigate inflammation and reduce bone resorption in experimentally induced apical periodontitis in rats, potentially holding promise for human endodontic disease treatment pending further research.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140177948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to assess the association between genetic polymorphisms in BMP2 (rs1005464 and rs235768), BMP4 (rs17563), SMAD6 (rs2119261 and rs3934908) and RUNX2 (rs59983488 and rs1200425) and pulp stones (PS). A total of 117 participants, consisting of 63 individuals with PS and 54 without PS, were included. Digital radiographs and a demographic/clinical questionnaire were used. Genomic DNA from salivary cells was genotyped via real-time polymerase chain reaction. Statistical analyses, including Chi-Square, Fisher's exact tests, Poisson regression and dimensionality reduction, were conducted. The rs2119261 polymorphism in the SMAD6 gene showed an association with genotype distribution in the recessive model (p = 0.049). The T–T haplotype in the SMAD6 gene (rs2119261 and rs3934908) was more prevalent in the control group and significantly linked with PS (p = 0.029). No associations were found between PS risk and genetic polymorphisms in BMP2, BMP4 and RUNX2. Polymorphisms in the SMAD6 gene were associated with PS.
{"title":"Investigation of polymorphisms in BMP2, BMP4, SMAD6 and RUNX2 genes and pulp stones","authors":"Katherine Azevedo Batistela Rodrigues Thuller DDS, MSc, Lívia Azeredo Alves Antunes DDS, MSc, PhD, Prescila Mota de Oliveira Kublitski DDS, MSc, PhD, João Armando Brancher DDS, MSc, PhD, Flares Baratto-Filho DDS, MSc, PhD, Erika Calvano Küchler DDS, MSc, PhD, Alice Corrêa Silva-Sousa DDS, MSc, Manoel Damião Sousa-Neto DDS, MSc, PhD, Marilisa Carneiro Leão Gabardo DDS, MSc, PhD, Leonardo Santos Antunes DDS, MSc, PhD","doi":"10.1111/aej.12835","DOIUrl":"10.1111/aej.12835","url":null,"abstract":"<p>This study aimed to assess the association between genetic polymorphisms in <i>BMP2</i> (rs1005464 and rs235768), <i>BMP4</i> (rs17563), <i>SMAD6</i> (rs2119261 and rs3934908) and <i>RUNX2</i> (rs59983488 and rs1200425) and pulp stones (PS). A total of 117 participants, consisting of 63 individuals with PS and 54 without PS, were included. Digital radiographs and a demographic/clinical questionnaire were used. Genomic DNA from salivary cells was genotyped via real-time polymerase chain reaction. Statistical analyses, including Chi-Square, Fisher's exact tests, Poisson regression and dimensionality reduction, were conducted. The rs2119261 polymorphism in the <i>SMAD6</i> gene showed an association with genotype distribution in the recessive model (<i>p</i> = 0.049). The T–T haplotype in the <i>SMAD6</i> gene (rs2119261 and rs3934908) was more prevalent in the control group and significantly linked with PS (<i>p</i> = 0.029). No associations were found between PS risk and genetic polymorphisms in <i>BMP2</i>, <i>BMP4</i> and <i>RUNX2</i>. Polymorphisms in the SMAD6 gene were associated with PS.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140095195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}