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Effect of radiopacifier and liquid in the physicochemical and biological properties of calcium silicate clinker Angelus: A laboratory investigation 放射性奶嘴和液体对硅酸钙熟料Angelus物理化学和生物性能的影响:实验室研究。
IF 1.6 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-10-30 DOI: 10.1111/aej.12808
Mário Tanomaru-Filho DDS, PhD, Bruna Vasconcellos de Oliveira DDS, Karina Ines Medina Carita Tavares DDS, MSc, Elisandra Márcia Rodrigues DDS, PhD, Fernanda Ferrari Esteves Torres DDS, PhD, Juliane Maria Guerreiro-Tanomaru DDS, PhD

The aim of this study was to evaluate the effect of radiopacifier calcium tungstate and manipulation with distilled water (DW) or liquid with additives (LA) on calcium silicate clinker Angelus (CL) properties, compared with MTA (Angelus, Brazil) and MTA Repair HP (MTAHP, Angelus, Brazil). The physicochemical properties, cellular viability and bioactivity were evaluated. ANOVA/Tukey and Bonferroni tests were performed (α = 0.05). There was no difference in material setting time (p > 0.05). MTA and MTAHP were similar (p > 0.05) and had greater radiopacity than CL + DW and CL + LA (p < 0.05). All experimental materials showed mass increase, alkalinisation capacity, besides biocompatibility and bioactivity at 3 and 7 days. The different liquids had no influence in the biological properties and bioactivity of the calcium silicate clinker Angelus. Calcium tungstate provided radiopacity, without changing the setting time, maintaining the mass increase and alkalinisation ability of the calcium silicate materials.

本研究的目的是与MTA(Angelus,Brazil)和MTA Repair HP(MTAHP,Angelus,巴西)相比,评估放射性安抚剂钨酸钙和蒸馏水(DW)或添加添加剂的液体(LA)对硅酸钙熟料Angelus(CL)性能的影响。对其理化性质、细胞活力和生物活性进行了评价。进行ANOVA/Tukey和Bonferroni检验(α = 材料凝固时间差异无统计学意义(p > MTA和MTAHP相似(p > 0.05),并且具有比CL更大的辐射不透性 + DW和CL + LA(p
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引用次数: 0
The impact of long-term storage conditions on the development of experimental dentinal microcracks 长期储存条件对实验性牙本质微裂纹发育的影响。
IF 1.6 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-10-24 DOI: 10.1111/aej.12806
Gustavo De-Deus PhD, Fabio Nakao Arashiro PhD, Erick Miranda Souza PhD, Emmanuel João Nogueira Leal Silva PhD, Tiago de Mello Guimarães MSc, Ana Carolina de Carvalho Maciel PhD, Diogo da Silva Oliveira PhD, Felipe Gonçalves Belladonna PhD, Ricardo Tadeu Lopes PhD, Marco Aurélio Versiani PhD

This study evaluated the impact of long-term storage conditions (medium and time) on the development of experimental dentinal microcracks through micro-computed tomography. Sixty freshly extracted premolars were stored in formalin, water, or dry conditions (n = 20) and scanned after 72 h, 30 days, 6 months and 3 years of extraction. The effect of the storage medium and time on the occurrence of dentinal defects was statistically evaluated. A total of 211 000 images were screened revealing the existence of 11 519 slices with dentinal defects. Dry conditions significantly contributed to the development of new defects in all time points. During the 3-year follow-up period, no new defects were detected in the teeth that were stored in water and, in a single tooth, in the formalin group, after 6 months of storage.

本研究通过微型计算机断层扫描评估了长期储存条件(介质和时间)对实验性牙本质微裂纹发展的影响。将60颗新鲜提取的前磨牙储存在福尔马林、水或干燥条件下(n = 20) 72后扫描 h、 30 天,6 月和3 多年的开采。对储存介质和时间对牙本质缺陷发生的影响进行统计学评估。共211 000张图像被筛选出来,揭示了11 519片牙本质缺损。干燥条件在所有时间点都显著促进了新缺陷的发展。在3年的随访期内,储存在水中的牙齿没有发现新的缺陷,福尔马林组在6 储存数月。
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引用次数: 0
Syringe irrigation in confluent canals: A sequential computational fluid dynamics assessment 合流管中的注射器冲洗:连续计算流体动力学评估。
IF 1.6 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-10-17 DOI: 10.1111/aej.12805
Mário Rito Pereira DDS, MSc, Goncalo Silva PhD, Viriato Semiao MSc, PhD, Jorge N. R. Martins DDS, MSc, PhD, Vania Silverio PhD, Paula Pascoal-Faria PhD, Isabel Duarte MSc, PhD, Nuno Alves PhD, António Ginjeira MD, DMD, PhD

This study aims to assess the influence of root canal preparation, irrigation needle design and its placement depth in the irrigation flow of confluent canals during syringe irrigation. A mandibular molar presenting two confluent canals in its mesial root was sequentially prepared and scanned by micro-computed tomography after mechanical preparation up to ProTaper Next system sizes X2 (25/.06v), X3 (30/.07v) and X4 (40/.06v). In each of the root canal preparation models, a side-vented and an open-ended needle at 5, 3 and 2 mm from the working length were included, and irrigation flow was assessed by a validated computational fluid dynamics model. The results revealed that the irrigant flowed out of the confluent canals mainly through the canal that did not have the needle. Apical penetration and renewal of the irrigant were most efficiently achieved with the use of a 30G open-ended needle and a 30/.07v preparation.

本研究旨在评估根管预备、冲洗针设计及其放置深度对注射器冲洗过程中合流管冲洗流量的影响。在机械制备至ProTaper Next系统尺寸X2(25/.06v)、X3(30/.07v)和X4(40/.06v)后,依次制备在近中根有两个合流管的下颌磨牙,并通过微型计算机断层扫描进行扫描 mm,并通过经验证的计算流体动力学模型评估灌溉流量。结果表明,冲洗剂主要通过没有针头的管道流出汇流管。使用30G开放式针头和30/.07v制剂最有效地实现了冲洗剂的顶端渗透和更新。
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引用次数: 0
Influence of two kinematics on canal transportation and centering ability of WaveOne Gold and One Curve files 两种运动学对WaveOne Gold和One Curve文件的运河运输和定心能力的影响。
IF 1.6 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-10-17 DOI: 10.1111/aej.12804
Raghda A. K. Almazedi BDS, MSc, Iman M. Al-Zaka BDS, MSc

This research examines canal transportation and centering ability of single-file systems when operated in continuous rotation or reciprocation. Sixty J-shaped resin blocks were used. The specimens were divided into two main groups (n = 30). Then, based on the motion used to operate the instruments, each group was subdivided into two subgroups (n = 15). Group A1: One Curve/rotation, Group A2: One Curve/clockwise reciprocation, Group B1: WaveOne Gold/Reciprocation, Group B2: WaveOne Gold/counter-clockwise rotation. Pre- and post-instrumentation images were obtained using digital microscope. Canal transportation was measured at five levels. A non-significant difference (p > 0.05) resulted between two motions at all levels. However, group A1 showed significantly less transportation at levels 3 and 4 (p ≤ 0.05), also more centered preparation at level 4 (p ≤ 0.05) than group B1. Within the limitations of this study, the apical transportation and centering ability of single-file systems are unaffected by different kinematics.

本研究考察了单文件系统在连续旋转或往复运动中运行时的运河运输和居中能力。使用了60个J形树脂块。标本分为两个主要组(n = 30)。然后,根据操作仪器的动作,每组被细分为两个亚组(n = 15) 。A1组:一条曲线/旋转,A2组:一个曲线/顺时针往复,B1组:WaveOne Gold/往复,B2组:Wave One Gold/逆时针旋转。使用数字显微镜获得仪器前后的图像。运河运输在五个层面进行了测量。无显著性差异(p > 0.05)在所有水平的两个运动之间产生。然而,A1组在第3级和第4级的运输量显著减少(p ≤ 0.05),在第4级的准备也更集中(p ≤ 0.05)显著高于B1组。在本研究的限制范围内,单个文件系统的顶端传输和居中能力不受不同运动学的影响。
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引用次数: 0
Cytokine expression of IL-8 before and after root canal treatment in patients with symptomatic apical periodontitis: A clinical trial 症状性根尖周炎患者根管治疗前后IL-8的细胞因子表达:一项临床试验。
IF 1.6 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-10-11 DOI: 10.1111/aej.12803
Sarah Samir Abouelenien MSc, PhD, Olfat Gamil Shaker MSc, PhD, Dina Ahmed Morsy MSc, PhD

This study's objective was to compare the cytokine expression of IL-8 in periapical tissues of single-rooted teeth with symptomatic apical periodontitis (SAP) before and after root canal treatments. As well as, comparing IL-8 levels in peri-apical tissues between vital and necrotic teeth with SAP.

Methodology

Thirty-six patients were allocated according to their pulp status into two experimental groups (n = 18) receiving the same treatment protocol; group 1: Vital pulps with SAP, and group 2: non-vital pulps with SAP. Conventional endodontic treatment was done on two visits; isolation and disinfection of the operative field were undertaken, and two-stage access cavity preparation was implemented. The first pre-instrumentation peri-apical sample (S1) was collected prior to cleaning and shaping procedures. A 2.5% NaOCl irrigation was used to thoroughly irrigate the canal after performing root canal preparation utilising the ProTaper Next (PTN) rotary system. After 1 week, the second post-instrumentation peri-apical sample (S2) was collected. Using an ELISA kit, the quantity of IL-8 was evaluated following the collection of all samples.

Results

In all pre-instrumentation samples, IL-8 was detected (100%). The level of IL-8 expression was significantly decreased from the S1 to S2 of all samples (p < 0.001). The intra-group comparison showed a statistically significant reduction in the level of IL-8 expression between S1 and S2 in both vital and non-vital groups where p < 0.001* in both groups. The inter-group comparison of levels of IL-8 expression (vital and non-vital) revealed a significant difference between both groups regarding the pretreatment sample with the higher levels of IL-8 shown in the non-vital group (p < 0.001). While in the post-treatment sample, both groups showed a significant reduction in the level of IL-8 expression but the difference between them was not statistically significant (p = 0.226).

Conclusion

Root canal instrumentation seems to be efficient in decreasing the levels of anti-inflammatory cytokines, namely IL-8. Further research should clarify how intra-canal medicaments affect inflammatory mediator levels.

本研究的目的是比较根管治疗前后单根牙和症状性根尖周炎(SAP)根尖组织中IL-8的细胞因子表达。方法:36例患者根据牙髓状态分为两组(n = 18) 接收相同的治疗方案;第1组:SAP的重要牙髓,第2组:SAP非重要牙髓。常规根管治疗在两次就诊中进行;对手术区进行了隔离和消毒,并进行了两阶段的进入腔准备。在清洁和整形程序之前采集第一个器械植入前根尖周样本(S1)。在使用ProTaper Next(PTN)旋转系统进行根管预备后,使用2.5%NaOCl灌溉来彻底灌溉根管。1之后 第二周,采集器械植入后的根尖周样本(S2)。使用ELISA试剂盒,在收集所有样品后评估IL-8的量。结果:在所有仪器前样品中,均检测到IL-8(100%)。所有样品的IL-8表达水平从S1到S2都显著降低(p 结论:根管内固定治疗可有效降低抗炎细胞因子IL-8的水平。进一步的研究应该阐明管内药物如何影响炎症介质水平。
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引用次数: 0
Evaluation of the effect of graphene oxide-based nanocomposites on smear layer by a scanning electron microscope: Laboratory investigation 用扫描电子显微镜评价氧化石墨烯基纳米复合材料对涂抹层的影响:实验室研究。
IF 1.6 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-10-06 DOI: 10.1111/aej.12802
Gulbin Sehitoglu DDS, Fatih Cakici DDS, PhD, Saniye Soylemez PhD, Cagatay Dengiz DSc

This study is to examine the effect of graphene oxide (GO) and GO-based nanocomposites (GO_EDTA, GO_CHIT) on the smear layer by scanning electron microscopy (SEM). Sixty human single-rooted anterior teeth were used. Samples were randomly categorised into six groups. Distilled water in group 1, 17% EDTA in group 2, chitosan (CHIT) solution in group 3, GO solution in group 4, GO_EDTA solution in group 5 and GO_CHIT in group 6 were used as irrigation solutions. Photomicrographs were acquired from the coronal, middle and apical parts of the samples by SEM. Statistical analyses were performed using Kruskal-Wallis, Mann–Whitney U and Wilcoxon tests. There was a statistically significant difference among the groups (p < 0.003). Adding GO to CHIT and EDTA did not affect removing the smear layer in all segments (p < 0.003). Incorporating GO into EDTA and CHIT solutions did not change their interaction with the smear layer.

本研究通过扫描电子显微镜(SEM)研究了氧化石墨烯(GO)和GO基纳米复合材料(GO_EDTA,GO_CHIT)对涂抹层的影响。使用了60颗人类单根前牙。样本被随机分为六组。用蒸馏水(第1组)、17%EDTA(第2组)、壳聚糖(CHIT)溶液(第3组)、GO溶液(第4组)、GO_EDTA溶液(第5组)和GO_CHIT(第6组)作为灌溉溶液。通过SEM从样品的冠状、中间和顶端获取显微照片。使用Kruskal-Wallis、Mann-Whitney U和Wilcoxon检验进行统计分析。各组间差异有统计学意义(p
{"title":"Evaluation of the effect of graphene oxide-based nanocomposites on smear layer by a scanning electron microscope: Laboratory investigation","authors":"Gulbin Sehitoglu DDS,&nbsp;Fatih Cakici DDS, PhD,&nbsp;Saniye Soylemez PhD,&nbsp;Cagatay Dengiz DSc","doi":"10.1111/aej.12802","DOIUrl":"10.1111/aej.12802","url":null,"abstract":"<p>This study is to examine the effect of graphene oxide (GO) and GO-based nanocomposites (GO_EDTA, GO_CHIT) on the smear layer by scanning electron microscopy (SEM). Sixty human single-rooted anterior teeth were used. Samples were randomly categorised into six groups. Distilled water in group 1, 17% EDTA in group 2, chitosan (CHIT) solution in group 3, GO solution in group 4, GO_EDTA solution in group 5 and GO_CHIT in group 6 were used as irrigation solutions. Photomicrographs were acquired from the coronal, middle and apical parts of the samples by SEM. Statistical analyses were performed using Kruskal-Wallis, Mann–Whitney U and Wilcoxon tests. There was a statistically significant difference among the groups (<i>p</i> &lt; 0.003). Adding GO to CHIT and EDTA did not affect removing the smear layer in all segments (<i>p</i> &lt; 0.003). Incorporating GO into EDTA and CHIT solutions did not change their interaction with the smear layer.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"50 1","pages":"3-14"},"PeriodicalIF":1.6,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41155169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Endodontic management of an aberrant germinated composite odontome: A case report 异常发芽复合齿组的牙髓治疗:一例报告。
IF 1.6 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-10-04 DOI: 10.1111/aej.12801
Ankit Arora BDS, MDS, Kavina Desai BDS, Sonali Kapoor BDS, MDS, Seema Gajera BDS

To describe the endodontic treatment of a germinated composite odontome with atypical internal anatomy. A 35-year-old male presented with pain in the left mandibular second molar region. Intraoral examination and Cone-Beam Computed Tomography (CBCT) revealed a rare case of a germinated composite odontome with a 3-2-1 Vertucci configuration in the mesial root. Occlusal morphology and mesial root configuration required modifications in methods of isolation and access cavity preparation. Contemporary tools like magnification, ultrasonics and controlled memory rotary files were essential for executing the treatment plan. The tooth was functional at 18 months follow up and radiographs revealed no abnormality.

描述具有非典型内部解剖结构的发芽复合齿体的牙髓治疗。一名35岁男性,左下颌第二磨牙区域疼痛。口腔内检查和锥形束计算机断层扫描(CBCT)显示了一例罕见的近中根有3-2-1 Vertucci结构的发芽复合齿组病例。咬合形态和近中根形态需要改变隔离和进入腔的准备方法。放大、超声波和可控记忆旋转锉等现代工具对执行治疗计划至关重要。这颗牙齿在18岁时就有了功能 随访数月,x线片未见异常。
{"title":"Endodontic management of an aberrant germinated composite odontome: A case report","authors":"Ankit Arora BDS, MDS,&nbsp;Kavina Desai BDS,&nbsp;Sonali Kapoor BDS, MDS,&nbsp;Seema Gajera BDS","doi":"10.1111/aej.12801","DOIUrl":"10.1111/aej.12801","url":null,"abstract":"<p>To describe the endodontic treatment of a germinated composite odontome with atypical internal anatomy. A 35-year-old male presented with pain in the left mandibular second molar region. Intraoral examination and Cone-Beam Computed Tomography (CBCT) revealed a rare case of a germinated composite odontome with a 3-2-1 Vertucci configuration in the mesial root. Occlusal morphology and mesial root configuration required modifications in methods of isolation and access cavity preparation. Contemporary tools like magnification, ultrasonics and controlled memory rotary files were essential for executing the treatment plan. The tooth was functional at 18 months follow up and radiographs revealed no abnormality.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"49 3","pages":"684-689"},"PeriodicalIF":1.6,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41156455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Finite element stress analysis of dental cement application on endocrown and onlay restoration 牙水泥应用于牙冠内嵌修复体的有限元应力分析。
IF 1.6 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-10-03 DOI: 10.1111/aej.12800
Satrio Wicaksono PhD, Wandi Prasetia MSc, Anna Muryani Sp KG, Subsp KE(K), Tatacipta Dirgantara PhD, Andi Isra Mahyuddin PhD

The aim of the study was to evaluate the influence of resin cement material types on tooth with endocrown and onlay restorations. The first molar was scanned using Micro-CT and underwent a modelling process to obtain the 3D model for computational simulation. Eight models were simulated in the current study with two loading conditions (720N vertical load and 200N oblique load), two types of restoration (onlay and endocrown restorations), and two resin cement variants (dual-cure resin cement and light-cure resin cement). The tooth with onlay restoration showed a significant stress reduction (up to 70%) when using light-cure resin cement compared to dual-cure resin cement. In contrast, types of cement did not affect stress distribution in the tooth with endocrown restoration. The current study found that dual-cure resin cement was preferable in Endocrown and Onlay restorations, due to dual-cure resin cement provided better bond strength compared to light-cure resin cement.

本研究的目的是评估树脂水泥材料类型对内冠和内嵌修复体牙齿的影响。使用Micro-CT扫描第一颗臼齿,并进行建模过程以获得用于计算模拟的3D模型。在当前的研究中,模拟了八个模型,分别具有两种载荷条件(720N垂直载荷和200N倾斜载荷)、两种类型的修复体(嵌体和内冠修复体)和两种树脂水泥变体(双固化树脂水泥和光固化树脂水泥)。与双固化树脂水泥相比,当使用光固化树脂水泥时,具有在线修复的牙齿显示出显著的应力降低(高达70%)。相反,骨水泥的类型不影响内冠修复牙齿的应力分布。目前的研究发现,双固化树脂水泥在Endocrown和Onlay修复体中更为可取,因为与光固化树脂水泥相比,双固化的树脂水泥提供了更好的粘结强度。
{"title":"Finite element stress analysis of dental cement application on endocrown and onlay restoration","authors":"Satrio Wicaksono PhD,&nbsp;Wandi Prasetia MSc,&nbsp;Anna Muryani Sp KG, Subsp KE(K),&nbsp;Tatacipta Dirgantara PhD,&nbsp;Andi Isra Mahyuddin PhD","doi":"10.1111/aej.12800","DOIUrl":"10.1111/aej.12800","url":null,"abstract":"<p>The aim of the study was to evaluate the influence of resin cement material types on tooth with endocrown and onlay restorations. The first molar was scanned using Micro-CT and underwent a modelling process to obtain the 3D model for computational simulation. Eight models were simulated in the current study with two loading conditions (720N vertical load and 200N oblique load), two types of restoration (onlay and endocrown restorations), and two resin cement variants (dual-cure resin cement and light-cure resin cement). The tooth with onlay restoration showed a significant stress reduction (up to 70%) when using light-cure resin cement compared to dual-cure resin cement. In contrast, types of cement did not affect stress distribution in the tooth with endocrown restoration. The current study found that dual-cure resin cement was preferable in Endocrown and Onlay restorations, due to dual-cure resin cement provided better bond strength compared to light-cure resin cement.</p>","PeriodicalId":55581,"journal":{"name":"Australian Endodontic Journal","volume":"49 3","pages":"665-674"},"PeriodicalIF":1.6,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41153676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Nd: YAG laser on tumour necrosis factor-alpha, interleukin-1beta and interferon-gamma levels in teeth with apical periodontitis: A clinical study Nd:YAG激光对根尖周炎患者牙齿肿瘤坏死因子-α、白细胞介素-β和干扰素-γ水平的影响:一项临床研究。
IF 1.6 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-09-25 DOI: 10.1111/aej.12799
Sevda Durust Baris DMD, Ali Turkyilmaz DMD, PhD, Mehmet Kursat Derici MD
This study aimed to evaluate the levels of tumour necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β) and interferon-gamma (IFN-γ) with asymptomatic apical periodontitis (AP). A total of 60 participants were randomly divided into two groups: the conventional irrigation (control) and the Nd: YAG laser irradiation. The interstitial fluids were obtained after root canal cleaning (day 0) and 1 week later (day 7). The TNF-α, IL-1β and IFN-γ levels were assayed using the enzyme-linked immunosorbent assay. The Mann-Whitney U, continuity correction chi-square, Pearson chi-square and Fisher exact tests were used. An increased level of cytokines on day 7 in the control group was observed, without statistically significant differences (p > 0.05). All cytokine levels decreased over time in the laser group. Only the IL-1β level showed a significant difference (p < 0.05). Nd: YAG irradiation has a positive effect on decreasing the proinflammatory cytokine level and may help to control infection in teeth with AP.
本研究旨在评估无症状根尖周炎(AP)患者的肿瘤坏死因子-α(TNF-α)、白细胞介素-β(IL-1β)和干扰素-γ(IFN-γ)水平。共有60名参与者被随机分为两组:常规冲洗(对照)和Nd:YAG激光照射。在根管清洁后(第0天)和第1天获得间质液 一周后(第7天)。采用酶联免疫吸附法测定TNF-α、IL-1β和IFN-γ水平。使用Mann-Whitney U、连续性校正卡方、Pearson卡方和Fisher精确检验。对照组在第7天观察到细胞因子水平升高,没有统计学显著差异(p > 0.05)。激光组中所有细胞因子水平随时间降低。只有IL-1β水平有显著差异(p
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引用次数: 0
Single nucleotide polymorphisms in inducible nitric oxide synthase gene are not associated with persistent apical periodontitis 诱导型一氧化氮合酶基因单核苷酸多态性与持续性根尖周炎无关。
IF 1.6 4区 医学 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Pub Date : 2023-09-19 DOI: 10.1111/aej.12796
Sandra Regina Santos Meyfarth DDS, MSc, Lívia Azeredo Alves Antunes DDS, MSc, PhD, Jhenyfer da Silva Tavares BSc, Ludmila da Silva Guimarães DDS, MSc, PhD, Erlange Andrade Borges da Silva DDS, MSc, PhD, Flares Baratto-Filho DDS, MSc, PhD, Erika Calvano Küchler DDS, MSc, PhD, Alice Corrêa Silva-Sousa DDS, MSc, PhD, Manoel Damião Sousa-Neto DDS, MSc, PhD, Leonardo Santos Antunes DDS, MSc, PhD

The aim of this study was to investigate whether there is an association between inducible in single nucleotide polymorphisms in nitric oxide synthase (rs2297518 and rs2779249) and persistent apical periodontitis. A total of 291 Brazilian subjects were included: 125 with signs/symptoms of persistent apical periodontitis and 166 with root canal-treated teeth exhibiting healthy perirradicular tissues. Endodontically treated patients were followed up after 1 year. The two single nucleotide polymorphisms in nitric oxide synthase were analysed using real-time polymerase chain reaction. Chi-square test and odds ratio with 95% confidence intervals were performed to compare genotype distributions between ‘healed’ and ‘persistent apical periodontitis’ groups (p < 0.05). Logistic regression analysis was used to evaluate SNP–SNP interactions. The allele and genotype distributions for the polymorphisms between the persistent apical periodontitis and healed groups were not statistically significant (p > 0.05). In the logistic regression analysis, the polymorphisms were not associated with persistent apical periodontitis and SNP–SNP interactions.

本研究的目的是调查一氧化氮合酶(rs2297518和rs2779249)中可诱导的单核苷酸多态性与持续性根尖周炎之间是否存在关联。共纳入291名巴西受试者:125名有持续性根尖周炎的体征/症状,166名有根管治疗的牙齿显示出健康的眶周组织。接受牙髓治疗的患者在1 年利用实时聚合酶链式反应分析了一氧化氮合酶的两个单核苷酸多态性。卡方检验和95%置信区间的比值比用于比较“治愈”和“持续性根尖周炎”组之间的基因型分布(p  0.05)。在逻辑回归分析中,多态性与持续性根尖周炎和SNP-SNP相互作用无关。
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引用次数: 0
期刊
Australian Endodontic Journal
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