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Influence of duration of storage and pre-sowing electromagnetic treatment on the development of the primary root system of cotton seeds 贮藏期和播前电磁处理对棉花种子主根系统发育的影响
Pub Date : 2021-12-01 DOI: 10.15547/ast.2021.04.064
M. Koleva, M. Radevska
Abstract. Seeds of five Bulgarian cotton varieties, stored for one and two years, were subjected to five pre-sowing electromagnetic treatments with different intensity and duration of exposure. It was found that the selected values of controllable factors had stimulating effect on the total length and total mass of sprout and root. The total length of sprout and root increased by 7.5-16.4% (p<0.05), the total mass of sprout and root increased by 7.8-12.7% (p<0.05). The best treatment options were 1[U=(8…5)kV, τ =(15…35)s] and 4[U=(6…3)kV, τ =(5…25)s]. Electromagnetic treatments had stronger positive effect on seeds stored for one year. Compared to the untreated control, corresponding to each storage period, the electromagnetic impact had stimulating effect for both storage periods: for the total length of sprout and root the increase was by 7.6-21.1% and 2.3-11.0% at option 4; for the total mass of sprout and root it was by 10.8-16.5% at option 1 and 4.5-9.1% at option 4, respectively, at one-year and two-year storage of seeds. In comparison with the control Chirpan-539 variety, untreated seeds, one-year storage, higher values for both studied characteristics were found only for seeds stored for one-year: for the total length of root and sprout at the varieties Natalia, treatment options 1 and 4, Nelina and Helius, options 2 and 4 and Chirpan-539, option 1; for the total mass of root and sprout at the varieties Nelina, options 1, 2 and 3 and Chirpan-539, option 5. Compared to the untreated control corresponding to each variety, the stimulating effect of the pre-sowing electromagnetic treatment for the two characteristics was found for all varieties, for both storage periods. The Helius variety was the most responsive to the electromagnetic impact, with the strongest stimulating effect for both characteristics, at the one-year storage of seeds the total length of sprout and root increased most strongly by 34.8-43.9% at options 1, 4 and 2[U=(6…3)kV, τ =(15…35)s], and the total mass of sprout and root increased by 48.5% at options 1.
摘要对储存一年和两年的五个保加利亚棉花品种的种子进行了五次不同强度和暴露时间的播种前电磁处理。结果表明,可控因子的选择值对芽和根的总长度和总质量有刺激作用。芽和根的总长度增加了7.5-16.4%(p<0.05),芽和根总质量增加了7.8-12.7%(p>0.05)。电磁处理对贮藏一年的种子有较强的正效应。与未处理的对照相比,对应于每个储存期,电磁冲击对两个储存期都有刺激作用:选择4时,芽和根的总长度分别增加了7.6-21.1%和2.3-11.0%;在种子贮藏一年和两年时,选择1和选择4的芽和根总质量分别提高了10.8-16.5%和4.5-9.1%。与对照Chirpan-539品种相比,未经处理的种子,一年储存,仅在储存一年的种子中发现两个研究特征的值更高:对于Natalia品种的根和芽的总长度,处理方案1和4,Nelina和Helius,方案2和4,以及Chirpan-593,方案1;对于品种Nelina(选项1、2和3)和Chirpan-539(选项5)的根和芽的总质量。与每个品种对应的未处理对照相比,在两个贮藏期内,播种前电磁处理对所有品种的两个特性都有刺激作用。Helius品种对电磁冲击的反应最为强烈,对这两个特性的刺激作用最强,在种子储存一年时,在选项1、4和2[U=(6…3)kV,τ=(15…35)s],芽和根的总长度增加了34.8-43.9%,而在选项1,芽和根部的总质量增加了48.5%。
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引用次数: 0
Somatic cells count in milk and its relation with productive traits in dairy cows 奶牛乳汁中体细胞计数及其与生产性状的关系
Pub Date : 2021-12-01 DOI: 10.15547/ast.2021.04.067
I. Marinov, D. Dimov, T. Penev
Abstract. The aim of the study was to determine the dependence between Test Day (TD) milk productivity traits and somatic cells count (SCC) in milk and also the influence of the factors: farm, parity and TD recording season of Holstein cows. The survey included a total of 484 lactating cows from 8 cattle farms in Bulgaria. A total of 3473 TD records including data on TD milk yield, fat %, protein % and SCC in milk were used. A statistically significant effect of the farm, recording season, and SCC on TD milk yield, fat % and protein % was reported. The highest TD milk yield was reported in spring (22.42 kg), followed by winter (21.95 kg). In winter and autumn were reported higher mean values for TD fat (3.77 and 3.84%) and TD protein (3.38 and 3.40%) content in milk. At the highest SCC – above 999 000 cells/ml, the highest average daily milk yield – 24.1 kg and the lowest fat (3.48%) was reported. With the highest and positive statistically significant value was the phenotypic correlation between TD milk yield and SCC (0.21). The correlation with TD fat % was statistically significant, with negative value (- 0.07).
摘要本研究旨在探讨荷斯坦奶牛产奶量性状与乳中体细胞数的相关性,以及乳场、胎次和产奶量记录季节等因素对产奶量的影响。该调查包括来自保加利亚8个养牛场的484头泌乳奶牛。共使用3473条TD记录,包括TD产奶量、乳中脂肪%、蛋白质%和SCC数据。据报道,农场、记录季节和SCC对TD产奶量、脂肪%和蛋白质%有统计学显著影响。产乳量在春季最高(22.42 kg),冬季次之(21.95 kg)。冬、秋季乳中脂肪(3.77)和蛋白质(3.38)含量平均值较高,分别为3.84%和3.84%。最高SCC高于999000细胞/ml时,平均日产奶量最高为24.1 kg,脂肪含量最低(3.48%)。TD产奶量与SCC的表型相关性最高(0.21),且具有正统计学意义。与TD脂肪%的相关性有统计学意义,为负值(- 0.07)。
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引用次数: 0
Efficacy of local Bacillus thuringiensis isolates against tomato leaf miner (Tuta absoluta) larvae on tomato plants under screenhouse conditions 在温室条件下,苏云金芽孢杆菌地方分离株对番茄潜叶虫幼虫的防治效果
Pub Date : 2021-12-01 DOI: 10.15547/ast.2021.04.066
E. O. Akinyelure, D. Machido, H. Atta
Abstract. The negative impact of chemical pesticides on the environment and the increased resistance of tomato leafminer (Tuta absoluta) field populations to chemical pesticides have promoted research on alternative control measures. Biological control with Bacillus thuringiensis (Bt) may be an alternative, especially against larval instars of T. absoluta. A total of five B. thuringiensis strains were isolated from soil sampled from two different Cow range lands in Zaria, Nigeria; and they were screened for the presence of the cry1 genes using polymerase chain reaction. Of the five isolates, two (40%) showed the presence of the cry1 genes. Results of the bioassay conducted against 2nd instar larvae of T. absoluta at 28±2°C indicated that each of the concentrations (25, 50, 75 and 100 ppm) of the spore crystal mixtures derived from the isolates harbouring cry1 genes caused significant mortality to larvae of T. absoluta after 72 hours in comparison to the control (0 ppm). Probit analysis was used to determine the LC50 and LT50 values. When the treatments were assessed at 48 and 72 hours, LC50 values against larvae were 74.1 and 25.3 ppm for isolate F3, while the LT50 values of that same isolate F3 at 100 ppm and 75 ppm were 36.3 and 42.7 hours, respectively. B. thuringiensis strain F2 achieved 68.7% reduction in T. absoluta damage on tomato plants, while B. thuringiensis isolate F3 achieved 71.3% reduction. Therefore, the spore crystal mixture derived from indigenous Bt strains is the candidate to be used for foliar application against T. absoluta and it is recommended into integrated pest control strategies for the management of T. absoluta.
摘要化学农药对环境的负面影响以及番茄潜叶蛾(Tuta absoluta)田间种群对化学农药的抗性增加,促进了对替代控制措施的研究。苏云金芽孢杆菌(Bt)的生物防治可能是一种替代方法,尤其是对绝对T.absoluta幼虫的防治。从尼日利亚扎里亚两个不同奶牛场的土壤样本中共分离出五株苏云金芽孢杆菌菌株;并使用聚合酶链式反应对它们进行cry1基因的存在筛选。在五个分离株中,有两个(40%)显示出cry1基因的存在。在28±2°C下对绝对T.absoluta 2龄幼虫进行的生物测定结果表明,与对照(0ppm)相比,来自携带cry1基因的分离株的孢子晶体混合物的每种浓度(25、50、75和100ppm)在72小时后对绝对T.bsoluta幼虫造成显著的死亡率。Probit分析用于确定LC50和LT50值。当在48和72小时评估处理时,分离物F3对幼虫的LC50值为74.1和25.3ppm,而相同分离物F3在100ppm和75ppm的LT50值分别为36.3和42.7小时。苏云金芽孢杆菌菌株F2对番茄植株的绝对T.absoluta损伤减少了68.7%,而苏云金杆菌分离株F3减少了71.3%。因此,来源于本土Bt菌株的孢子晶体混合物是用于叶面施用抗绝对T.absoluta的候选者,并被推荐为综合害虫防治策略来管理绝对T.abstrata。
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引用次数: 0
Response of durum wheat lines to the cause agent of leaf rust Puccinia triticina 硬粒小麦品系对叶锈病病原的反应
Pub Date : 2021-12-01 DOI: 10.15547/ast.2021.04.054
V. Ivanova, T. Alexandrov
Аbstract. The response of 1262 durum wheat lines to the cause agent of leaf rust Puccinia triticina was studied. The investigation was carried under infection field conditions during 2013-2015 at Dobrudzha Agricultural Institute (DAI) – General Toshevo, Bulgaria. Over the years of investigation, a maximum high infection background was developed by the cause agent of the disease, including all pathogen pathotypes identified during this period. The race variability in the population of the pathogen during the period was determined at the Plant Pathology Laboratory of DAI according to a validated methodology for working with rusts. Seventy-one phenotypically different virulent pathotypes were established. Fifteen new races of P. triticina were also identified, which have not been present during the previous 15 years in Bulgaria. Seventeen lines and one cultivar with a certain degree of resistance to the pathogen were selected. The selected lines can be involved in the breeding programs for developing initial material with resistance to leaf rust P. triticina.
Аbstract。研究了1262个硬粒小麦品系对叶锈病病原菌的反应。调查于2013-2015年在保加利亚托舍沃将军Dobrudzha农业研究所(DAI)的感染现场条件下进行。在多年的调查中,该疾病的病原形成了最高的高感染背景,包括在此期间确定的所有病原体病理类型。在此期间,在DAI植物病理学实验室根据一种有效的锈病处理方法确定了病原体种群的种族变异性。建立了71种表型不同的毒力型。还发现了15个新的小种,这些小种在保加利亚过去15年中没有出现过。选育了具有一定抗性的17个品系和1个品种。所选品系可参与抗小麦叶锈病初始材料的选育计划。
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引用次数: 0
Possibilities for integrated control of the cotton bollworm (Helicoverpa armigera Hübner, 1808) in the growing tomato in greenhouses 综合防治温室番茄中棉铃虫(Helicoverpa armigera Hübner,1808)的可能性
Pub Date : 2021-12-01 DOI: 10.15547/ast.2021.04.063
R. Mineva, V. Yankova, N. Valchev
Abstract. In recent years, there has been a significant increase in the number of cotton bollworm (Helicoverpa armigera Hb., 1808) due to changing climatic conditions, the covert life cycle of caterpillars and the formation of resistance in the populations to some of the commonly used insecticides. Experiments with tomato variety Pink Rock F1 grown in greenhouses to determine the biological activity of some products for plant protection against cotton bollworm were conducted at the “Maritsa” Vegetable Crops Research Institute – Plovdiv. The bioproducts Rapax, Dipel 2X, Helicovex, Neem Azal T/S and Sineis 480 SC have good effectiveness >77% towards cotton bollworm in the interval 7-14 days after treatment. The tested insecticides Lanate 25 WP, Coragen 20 SC 200 ml/ha, Exalt 25 SC, Voliam Targo 063 SC, Ampligo 150 ZC and Affirm 095 SG show very good biological activity (E>84%) 7-14 days after treatment.
摘要近年来,由于气候条件的变化、毛毛虫的隐蔽生命周期以及种群对一些常用杀虫剂形成耐药性,棉铃虫(Helicoverpa armigera Hb.,1808)的数量显著增加。普罗夫迪夫“Maritsa”蔬菜作物研究所对温室中种植的番茄品种Pink Rock F1进行了实验,以确定一些产品对植物保护免受棉铃虫侵害的生物活性。生物制品Rapax、Dipel 2X、Helicovex、Neem Azal T/S和Sineis 480 SC在处理后7-14天内对棉铃虫的有效性>77%。试验杀虫剂Lanate 25 WP、Coragen 20 SC 200 ml/ha、Exalt 25 SC、Voliam Targo 063 SC、Ampligo 150 ZC和Affirm 095 SG在处理后7-14天显示出非常好的生物活性(E>84%)。
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引用次数: 0
Allele frequencies and genotypes of kappa casein (CSN3) and their association with chemical composition and coagulation properties of milk in Brown cattle 褐牛kappa酪蛋白(CSN3)等位基因频率、基因型及其与牛奶化学成分和凝固特性的关系
Pub Date : 2021-12-01 DOI: 10.15547/ast.2021.04.057
T. Angelova, J. Krastanov, D. Yordanova
Abstract. The aim of the present study was to evaluate allele frequencies and genotypes of kappa casein (CSN3) and their association with milk quality and coagulation properties in Brown cattle. Milk proteins’ polymorphism was found out in 155 tissue samples from cows reared at 4 farms. The analysis of milk composition was done in the lab of the Agriculture Institute – Stara Zagora on Lactoscan ultrasound milk analyzer, whereas coagulation properties of individual milk samples were evaluated on a Computerized Renneting Metter – Polo Trade, Italy. Milk samples were obtained by milk meters. The milk was analysed within 3 hours after sample collection. Naturen Plus 215/0.8L chymosin was used, with milk coagulation activity of 215 IMCU/ml. During the study, the following parameters were studied: milk fat and protein contents (%), rennet coagulation time (RCT, min), curd firmness (а30, mm) and curd firming time (k20, min). Kappa casein (CSN3) is characterised by five genotypes – АА, АВ, ВВ, АН and ВН, the frequency of which varied within various ranges. The milk of cows with genotype AB was characterized by the highest content of fat and protein: 4.85% and 5.00%, respectively. The milk of heterozygous cows from genotype AB demonstrated the longest rennet coagulation time – 18.04 min. The animals carrying the H allele produced milk with the highest curd firmness – 37.00 mm.
摘要本研究的目的是研究kappa酪蛋白(CSN3)等位基因频率和基因型及其与棕色牛牛奶品质和凝血特性的关系。在4个农场饲养的155个奶牛组织样本中发现了乳蛋白的多态性。牛奶成分的分析是在农业研究所- Stara Zagora实验室的乳扫描超声牛奶分析仪上完成的,而单个牛奶样品的凝血特性是在计算机凝血仪上评估的- Polo贸易,意大利。牛奶样品由牛奶计量仪获得。样品采集后3小时内对牛奶进行分析。采用Naturen Plus 215/0.8 l凝乳酶,凝乳活性为215 IMCU/ml。在研究过程中,研究了以下参数:乳脂和蛋白质含量(%)、凝乳酶凝固时间(RCT, min)、凝乳硬度(30,mm)和凝乳凝固时间(k20, min)。Kappa酪蛋白(CSN3)具有АА、АВ、ВВ、АН和ВН五种基因型,其频率在不同范围内变化。AB型奶牛的乳汁脂肪和蛋白质含量最高,分别为4.85%和5.00%。AB型杂合奶牛的凝乳凝固时间最长,为18.04 min,携带H等位基因的奶牛的凝乳硬度最高,为37.00 mm。
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引用次数: 0
Differentiation of the indigenous goat populations in Ethiopia based on morphometric features and zoometric indices: The primary step for conservation 基于形态计量特征和动物计量指数的埃塞俄比亚土著山羊种群分化:保护的首要步骤
Pub Date : 2021-12-01 DOI: 10.15547/ast.2021.04.056
B. Tade, A. Melesse, S. Betsha
Abstract. This study was conducted to describe the indigenous goat populations of South Gondar, Ethiopia based on morphometric traits and body indices. To this effect, morphometric measurements were taken from 512 goats (153 male and 359 female) of both sexes drawn from three districts (Farta, Fogera and Libokemkem). Twenty structural indices were computed from morphometric measurements. Results indicated that age at first kidding and kidding intervals was 13.6 and 8.39 months, respectively, with an average litter size of 1.54 kids. Sex affected (p<0.001) all quantitative traits except ear length (EL), rump width (RW) and fore canon circumference (FCC) being higher in bucks than in does. The effect of age was significant (p<0.001) for all morphometric traits except for EL resulting in a linear increase with advancing age in both sexes. Except for EL and scrotal circumferences (SC), all quantitative traits were (p<0.01) affected by district of the goats rearing. Accordingly, Fogera goats had higher live weight (LW), heart girth (HG), height at withers (HW), body length (BL), chest width (CW), rump height (RH), rump length (RL) and teat length (TL) than those of Libokemkem. Fogera goats had also the highest LW and HG compared with those of Farta. Conversely, the Farta goats had higher RW, BL, TL, RL, CW, chest depth (CD), and paunch girth (PG) than those of Libokemkem. Libokemkem goats were inferior in most of the studied morphometric traits. Both HG and HW variables were identified as best predictors of LW in both sexes. Structural indices indicated that the goat populations could be characterized as meat phenotype with short legs being well adapted to midland altitudes. In conclusion, goats reared in the region could have a genetic potential for meat production with very good prolificacy. The Fogera goats were particularly better in most morphometric traits indicating their suitability for commercial meat production. However, further on-station research is recommended to verify their potentials as meat animals for enhanced food security in the region.
摘要本研究基于形态特征和身体指数对埃塞俄比亚南贡达尔地区的山羊种群进行了描述。为此,从三个地区(Farta、Fogera和Libokemkem)抽取的512只雌雄山羊(153只公山羊和359只母山羊)进行了形态计量学测量。从形态测量中计算了20个结构指数。结果表明,幼犬首次开玩笑年龄为13.6个月,开玩笑间隔时间为8.39个月,平均产仔数为1.54只。雄鹿除耳长(EL)、臀宽(RW)和前臀围(FCC)外,其他性状均受性别影响(p<0.001)。年龄对所有形态计量性状的影响均显著(p<0.001),但EL随年龄增长呈线性增加。除EL和阴囊周长(SC)外,其他数量性状均受饲养地区的影响(p<0.01)。因此,福格拉山羊的活重(LW)、胸围(HG)、肩高(HW)、体长(BL)、胸宽(CW)、臀高(RH)、臀长(RL)和乳长(TL)均高于利博凯肯山羊。与法尔塔山羊相比,福格拉山羊的LW和HG最高。相反,法尔塔山羊的RW、BL、TL、RL、CW、胸深(CD)和腹围(PG)均高于利博肯姆山羊。利博肯姆山羊的大部分形态计量性状都较差。HG和HW变量均被确定为两性LW的最佳预测因子。结构指标表明,山羊种群具有肉型表型,腿短,适应中部海拔高度。综上所述,该地区饲养的山羊具有良好的遗传潜力,具有良好的繁殖能力。福格拉山羊在大多数形态计量学性状上表现得特别好,表明它们适合商业肉类生产。但是,建议进行进一步的实地研究,以验证它们作为肉类动物的潜力,以加强该区域的粮食安全。
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引用次数: 0
Growth and yield of pomato under the Syrian coastal mountains conditions 叙利亚沿海山区条件下pomato的生长和产量
Pub Date : 2021-12-01 DOI: 10.15547/ast.2021.04.062
Alaa Suhiel Ibrahim
Abstract. The world has always been striving to increase and intensify agricultural production, and there are several attempts to acheive that, such as grafting tomato on potato to obtain two crops from one plant. This investigation was conducted during 2020 in open field in Nahl village, Baniyas, Tartous Governorate, Syria. The vegetative and generative growth and yield have been studied for pomato (grafted tomato on potato) and compared to each tomato and potato separately. Tomato plants were significantly superior in plant height to pomato plants after 23 days of planting until the end of the experiment. On the other hand, there were significant differences between tomato plants and pomato plants in the leaf area after 23, 51 and 65 days of planting. Also, tomato plants significantly outperformed pomato plants in the number of flowers and fruits per plant and the tomato fruit set percent. Anyway, tomato plants were significantly superior in the average yield of tomato fruits per plant (1657 g.plant-1) to pomato plants (185 g.plant-1), while there were insignificant differences in the average yield of potato tubers per plant between pomato which failed to produce tubers and potato (48.25 g.plant-1). Studying the tomato fruit fresh weight showed a significant superiority of tomato plants (54.37 g.fruit-1) to pomato plants (35.97 g.fruit-1), while there were insignificant differences in the other physical (tomato fruit height, diameter and shape index) and chemical (tomato fruit content of total acids, total soluble solids and dry matter, %) properties.
摘要世界上一直在努力增加和加强农业生产,并有几种尝试来实现这一目标,例如将番茄嫁接到马铃薯上,从一株植物获得两种作物。该调查于2020年期间在叙利亚塔尔图斯省巴尼亚斯Nahl村的露天场地进行。研究了马铃薯接枝番茄的营养生长、生殖生长和产量,并分别对番茄和马铃薯进行了比较。种植23 d至试验结束,番茄植株株高显著优于番茄植株。另一方面,种植23、51和65 d后,番茄植株与番茄植株的叶面积差异显著。此外,番茄植株在每株花和果实的数量以及番茄结实率方面明显优于番茄植株。结果表明,番茄单株平均果实产量(1657 g.plant-1)显著高于番茄单株平均产量(185 g.plant-1),而马铃薯块茎产量(48.25 g.plant-1)与不产块茎的番茄单株差异不显著。番茄果实鲜重研究表明,番茄植株(54.37 g.fruit-1)显著优于番茄植株(35.97 g.fruit-1),而其他物理性状(番茄果实高、直径和形状指标)和化学性状(番茄果实总酸、总可溶性固形物和干物质含量,%)差异不显著。
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引用次数: 0
Biochemical reference intervals in broiler chickens according to age and strain 肉鸡不同年龄和品系的生化参考区间
Pub Date : 2021-12-01 DOI: 10.15547/ast.2021.04.058
N. Arzour-Lakehal, A. Boudebza
Abstract. The objective of the research was to study the physiological pattern of biochemical variables and to obtain reference intervals for young (less than 1 month) and adult (more than 1 month) broiler chickens of 2 strains. From the jugular vein, blood for analysis was collected, separated, and then immediately analyzed. The influence of age was significant for the majority of the investigated variables in the 2 groups. In Isa15 strain, a significant age-related decrease in plasma uric acid, glucose, cholesterol, triglycerides, phosphorus, potassium and iron was established. Also, a significant age-related increase in plasma calcium and ASAT was obtained in the same broiler strain. In Arbor Acres Plus strain, a significant age-related decrease in plasma glucose, cholesterol, triglycerides and phosphorus was observed. A significant age-related increase in plasma total protein, calcium and ASAT has also been reported in this broiler strain. A significant difference between the two strains was reported for plasma uric acid, glucose, cholesterol, phosphorus, magnesium and iron (p<0.05). For most estimated parameters in the 2 strains, calculation of separate reference intervals for young and adult animals was appropriate. Except for potassium in young Isa15 strain, and phosphorus in young Arbor Acres Plus strain, all variables did not follow a normal distribution. Reference intervals are presented for both ages using non-parametric or robust method. 90% confidence intervals for both groups were calculated non-parametrically, or by the bootstraping method. The established reference intervals will be a useful guide for interpreting plasma biochemical variables in different strains of broiler chickens raised in Algeria, and reared in a mild Mediterranean climate.
摘要本研究旨在研究2个品系肉鸡仔鸡(小于1月龄)和成鸡(大于1月龄)的生化指标生理规律,并获得参考区间。从颈静脉中采集血液,分离,然后立即进行分析。在两组中,年龄对大多数被调查变量的影响是显著的。在Isa15菌株中,血浆尿酸、葡萄糖、胆固醇、甘油三酯、磷、钾和铁的下降与年龄显著相关。此外,在同一肉鸡品系中,血浆钙和ASAT均与年龄显著相关。在Arbor Acres Plus菌株中,观察到血浆葡萄糖、胆固醇、甘油三酯和磷的显著下降与年龄相关。在这一肉鸡品系中,血浆总蛋白、钙和ASAT也有与年龄相关的显著增加。两株血清尿酸、葡萄糖、胆固醇、磷、镁、铁含量差异有统计学意义(p<0.05)。对于2种菌株的大多数估计参数,分别计算幼畜和成年动物的参考区间是合适的。除Isa15幼株中钾元素和Arbor Acres Plus幼株中磷元素外,其余变量均不服从正态分布。采用非参数或鲁棒方法给出了两种年龄的参考区间。两组的90%置信区间均采用非参数或自举方法计算。建立的参考区间将为解释在阿尔及利亚饲养并在温和的地中海气候下饲养的不同品种肉鸡的血浆生化变量提供有用的指导。
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引用次数: 2
Evaluation of the antimicrobial and anti-Varroa destructor L. activity of the essential oil of clove (Syzygium aromaticum L. Myrtaceae) 丁香精油的抑菌活性及抗瓦螨活性评价
Pub Date : 2021-12-01 DOI: 10.15547/ast.2021.04.065
K. Mekademi, D. Kebour, N. Ouchene-khelifi, N. Ouchene
Abstract. Antimicrobial and anti-Varroa destructor L. activity of the essential oil (EO) of clove (Syzygium aromaticum L. Myrtaceae) was evaluated in this study. Antimicrobial activity concerned 9 bacterial strains (Bacillus cereus, Escherichia coli ATCC 25911, Staphylococcus aureus 29213 ATCC, Proteus mirabilis, Serratia marcescens, Enterobacter sakazakii, Pseudomonas aeruginosa ATCC, Escherichia coli and Acinetobacter sp.), a fungal strain (Penicillium sp.) and a yeast species (Candida albicans). The extraction of the EO was carried out by the method of hydrodistillation. Results showed that EO has no toxicity on bees Apis mellifera. EO demonstrated effective and stable anti-V. destructor activity, indicating the absence of possible resistance, in contrast to what was observed for Amitraz. The inhibitory activity of EO revealed an inhibition zone of diameter varying between 20 mm and 42 mm for bacteria. However, no inhibition zones were observed for Pseudomonas aeruginosa ATCC. The inhibitory activity of EO on Penicillium sp and Candida albicans revealed an inhibition zone of 39 mm in diameter. This study shows that the EO of cloves constitutes a simple and natural treatment, without inconveniences, with a high activity antimicrobial and anti-Varroa destructor which merits it to be proposed as a means to fight against varroosis and the tested pathogens.
摘要本研究评价了丁香(Syzygium aromaticum L.Myrtaceae)精油的抗菌和抗Varroa destructor L.活性。抗菌活性涉及9种细菌菌株(蜡样芽孢杆菌、大肠杆菌ATCC 25911、金黄色葡萄球菌29213 ATCC、奇异变形杆菌、粘质沙雷氏菌、阪崎肠杆菌、铜绿假单胞菌ATCC、大肠杆菌和不动杆菌。)、一种真菌菌株(青霉属)和一种酵母(白色念珠菌)。采用加氢蒸馏法提取EO。结果表明,EO对蜜蜂无毒害作用。EO显示出有效且稳定的抗-V。破坏活性,表明没有可能的耐药性,与阿米特拉兹的观察结果相反。EO的抑制活性揭示了对细菌直径在20mm和42mm之间变化的抑制区。然而,没有观察到铜绿假单胞菌ATCC的抑制区。EO对青霉和白色念珠菌的抑制活性显示直径为39mm的抑制区。这项研究表明,丁香的EO是一种简单自然的治疗方法,没有任何不便,具有高活性的抗菌和抗Varroa破坏剂,值得作为对抗varroosis和测试病原体的一种手段。
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Agricultural Science and Technology
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