微生物学(英文)最新文献

{"title":"Secalonic Acid and Benzoic Acid Analogues Exhibiting Cyto toxicity against Cancer Cells Isolated from <i>Claviceps yanagawaensis</i>","authors":"Yuji Doi, Daigo Wakana, S. Kitaoka, Hisashi Takeda, E. Tanaka, T. Hosoe","doi":"10.4236/aim.2022.1212045","DOIUrl":"https://doi.org/10.4236/aim.2022.1212045","url":null,"abstract":"","PeriodicalId":56408,"journal":{"name":"微生物学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70486567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-CoV-2 Infection Is Associated with Vitamin D Deficiency in Côte d’Ivoire","authors":"L. Boyvin, Y. G. Yayé, Gnogbo Alexis Bahi, Aya Jeanne Armande Aké, K. Séri, D. Sévédé, S. Eholie, M. Dosso, A. Djaman","doi":"10.4236/aim.2022.122004","DOIUrl":"https://doi.org/10.4236/aim.2022.122004","url":null,"abstract":"","PeriodicalId":56408,"journal":{"name":"微生物学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70486621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research status and prospect of the antibacterial effect of equol","authors":"奥 孙","doi":"10.12677/amb.2022.114024","DOIUrl":"https://doi.org/10.12677/amb.2022.114024","url":null,"abstract":"Equol, is a kind of phytoestrogenic compound, which synthesized by soybean isoflavones by intestinal microbiota. It has high affinity for estrogen receptor and higher biological activity than soybean isoflavones, so it is one of the widely studied small molecule substances. Equol has many biological effects, such as hormone like effect, antioxidation, immune regulation, etc. In this paper, the biological activity and antibacterial effect of equol were reviewed, in order to provide new ideas for the antibacterial treatment of equol.","PeriodicalId":56408,"journal":{"name":"微生物学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66128266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Summary and Prospect of Microbial Degradation of Roxarsone","authors":"祯 王","doi":"10.12677/amb.2022.114028","DOIUrl":"https://doi.org/10.12677/amb.2022.114028","url":null,"abstract":"Present, Roxarsone is the most economical organic arsenarsone preparation, which is widely used in the breeding industry as a feed additive. However, due to the low absorption and conversion efficiency of Roxarsone in livestock and poultry, a large amount of Roxarsone flows into the external environment along with excrement. Simple composting and conventional sewage treatment can‘t be completely removed, but discharged into water and soil, resulting in arsenic compound pollution. In addition, microorganisms or light in the environment can degrade Roxarsone into a variety of arsenic compounds, such as 4-hydroxy-3-aminobenzene arsenic acid (HAPA), which can cause serious harm to the environment and human body. At present, in addition to physical and chemical methods, microbial method also has a good degradation effect of Roxarsone. This paper reviewed the research progress on the non-biodegradation and biodegradation transformation of Roxarsone and its related products. The main conclusions are: the non-biodegradation and bio-degradation transformation of Roxarsone is greatly affected by environmental factors, and it is easy to produce more toxic pollutants. Biodegradation and transformation: the irreplaceable role of microorganisms is mainly reflected in: (1) Microbial adsorption and accumulation, including reversible and irreversible processes; (2) Bacterial degradation of Roxarsone; (3) Degradation of Roxarsone by fungi; (4) Degradation of Roxarsone by yeast. Finally, the prospect of applying microbial method to Roxarsone pollution remediation was discussed.","PeriodicalId":56408,"journal":{"name":"微生物学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66128346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amplification of Fluorescent Cy5DNA and Separation-Detection of Digestion Product","authors":"园霞 郑","doi":"10.12677/amb.2022.114030","DOIUrl":"https://doi.org/10.12677/amb.2022.114030","url":null,"abstract":"[Objective] Amplified with dNTPs added with a specific ratio of fluorescent Cy5-dATP, fluorescent Cy5DNA can assay exonuclease such as T5 DNA exonuclease (T5exo), key is separation-detection of digestion product. [Method] Amplified with dNTPs added with 1/1000 Cy5-dATP, a 5851 bp fluorescent Cy5DNA pET28a-xyn served for T5exo digestion. Basing on DNA-absorbing principle of DNA-clean column, a 2-flod buffer P3 (2×P3) that of DNA solution was determined as the least quantity of a column to absorb DNA. Next, a DNA-clean column with 2×P3 served to separate a so-lution containing Cy5DNAs or equal amount of Cy5DNAs digested by T5exo to collect filtrate and elute, respectively.[Result] Assayed by a microreader SpectraMax ® i3x, the filtrate and elute from 120 ng Cy5DNAs-T5exo solution had a fluorescence value of 7573 and 0, respectively. The elute and filtrate from 120 ng Cy5DNA solution had a fluorescence value of 5824 and 0, respectively. Assayed by a con-focal microscope, the filtrate from Cy5DNA-T5exo solution exhibited Cy5-dATP fluorescence, while the elute did not. The elute from Cy5DNA solution exhibited Cy5 fluorescence, while the filtrate did not. Both the quantity and the quality assay were in agreement with the principle and usage of DNA-clean column. Additionally, correlation was analyzed for concentration of Cy5DNA and Cy5-dATP with fluorescence intensity. [Conclusion] Cy5DNA pET28a-xyn was amplified successfully, and its T5exo digestion product Cy5-dATP was separated by a DNA-clean column with a 2×P3 buffer. The study provided a basis for fluorescent DNA amplification and usage in as-saying DNA-cutting enzyme.","PeriodicalId":56408,"journal":{"name":"微生物学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66128448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, Identification and Screening of Lactic Acid Bacteria with Potential Vaginal Health Care","authors":"佳琳 江","doi":"10.12677/amb.2022.111004","DOIUrl":"https://doi.org/10.12677/amb.2022.111004","url":null,"abstract":"","PeriodicalId":56408,"journal":{"name":"微生物学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66128557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discussion on the Treatment of Acne Vulgaris","authors":"诗航 唐","doi":"10.12677/amb.2022.112010","DOIUrl":"https://doi.org/10.12677/amb.2022.112010","url":null,"abstract":"","PeriodicalId":56408,"journal":{"name":"微生物学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66128235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress on the Infection of Intestinal Protozoa in HIV/AIDS Population","authors":"厉雨婷 周","doi":"10.12677/amb.2022.112014","DOIUrl":"https://doi.org/10.12677/amb.2022.112014","url":null,"abstract":"","PeriodicalId":56408,"journal":{"name":"微生物学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66128369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research Progress of SARS-CoV-2 Omicron Variant","authors":"康泓 王","doi":"10.12677/amb.2022.112006","DOIUrl":"https://doi.org/10.12677/amb.2022.112006","url":null,"abstract":"","PeriodicalId":56408,"journal":{"name":"微生物学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66128607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic Biology Construct of Ebola Virus in Bacteria Surrogate Is Stable and Safe for Rapid Detection Studies in a BSL-2 Laboratory Setting","authors":"N. Esiobu, D. Holmes, Chad T Coarsey, W. Asghar, Bodhi Stone","doi":"10.4236/aim.2022.121003","DOIUrl":"https://doi.org/10.4236/aim.2022.121003","url":null,"abstract":"Rapid detection of virulent pathogens during an outbreak is critical for public health advisories and control of the disease in a population. While many molecular techniques for point of care and clinical diagnosis abound, the US experience with the COVID-19 testing in the early stages of the pandemic underscores the critical importance of determining the appropriate target gene(s) with in-built controls that reliably detect pathogens with high sensitivity and specificity. Assays and research for diagnostics and therapy could be slowed during an epidemic because access to the required BSL-3 and BSL-4 laboratories are limited. So, during the 2014 West Africa Ebola outbreak, we tested the hypothesis that using synthetic cDNA of Ebolavirus in a bacteria surrogate (fit for all lab settings), would remain unmutated and safe after several generations, serving as an effective positive control in research settings, self test and point-of-care detection platforms. Primers were designed for the detection and quantification of the nucleoprotein (NP) gene of the 2014 Makona Ebola strain (KR781608.1, 733 1332 bp). To test the stability of artificially inserted translation arrest in the Orf of the model gene, it was edited to include three STOP codons in the RNA transcript using SNAP GENE. The segment was then spliced into a high copy number plasmid, cloned into One Shot TOP10 Escherichia coli (Invitrogen), and tested for stability and safety by periodic subculture, extraction and sequencing. Unlike COVID-19, rapid detection of blood-borne etiologies like Ebola requires optimized protocols for blood matrix. Using real-time PCR and newly designed primer pairs, the EBOV surrogate was detected and enumerated in human blood and regular broth and buffers. Based on aligned sequence analysis, the EBOV synthetic NP gene was stable (>99.9999% similarity coefficient) for at least 3 months. Detection sensitivity in broth and blood was at least 100 cells/ml or about 5.8 How to cite this paper: Esiobu, N., Holmes, D., Coarsey, C., Asghar, W. and Stone, B. (2022) Synthetic Biology Construct of Ebola Virus in Bacteria Surrogate Is Stable and Safe for Rapid Detection Studies in a BSL-2 Laboratory Setting. Advances in Microbiology, 12, 25-41. https://doi.org/10.4236/aim.2022.121003 Received: November 24, 2021 Accepted: January 25, 2022 Published: January 28, 2022 Copyright © 2022 by author(s) and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/","PeriodicalId":56408,"journal":{"name":"微生物学(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70486405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}