Pub Date : 2024-12-14DOI: 10.1134/S1061934824701326
Fengxia Cui, Ying Zhang, Yutong Liu, Hao Li
Misuse of chloramphenicol (CAP) could lead to its residue in animal-sourced food and subsequently harm human health through the food chain. Therefore, it is urgently needed to effectively determine CAP residues in animal-sourced food. Here, an easily operated target recycling strategy for the determination of CAP was developed based on a CAP-specific aptamer. The CAP-specific aptamer was hybridized with a complementary short chain modified with fluorescent and quenching groups at both ends. Upon addition of CAP, the aptamer would be separated from its complementary probe and bind with CAP. The fluorescent and quenching groups labeled on the complementary probe were separated by exonuclease I (Exo I) digestion to release a fluorescent signal. Meanwhile, Exo I also digested the aptamer, and CAP was released from the complex, triggering the determination signal amplification based on CAP recycling. Factors (i.e., NaCl concentration and Exo I digestion time) that would affect the determination performance of this sensor system were optimized. Under the optimized conditions, the developed sensor system exhibited high determination sensitivity toward CAP with a limit of detection of 0.08 ng/mL (linear range from 0.1 to 5 ng/mL). Moreover, this sensor system was applied to determine CAP in real spiked milk, and the recovery rates ranged from 97.2 to 100.0%. Meanwhile, this sensor system also suggested cost efficiency with a determination cost of only $0.29 per sample. Results presented here and the high specificity of the aptamer suggested by the previous work indicated the application potential of this developed aptamer-based sensor system for the determination of CAP residue in animal-sourced food.
{"title":"An Easily Operated Signal Amplification Sensor System for the Determination of Chloramphenicol Residues","authors":"Fengxia Cui, Ying Zhang, Yutong Liu, Hao Li","doi":"10.1134/S1061934824701326","DOIUrl":"10.1134/S1061934824701326","url":null,"abstract":"<p>Misuse of chloramphenicol (<b>CAP</b>) could lead to its residue in animal-sourced food and subsequently harm human health through the food chain. Therefore, it is urgently needed to effectively determine CAP residues in animal-sourced food. Here, an easily operated target recycling strategy for the determination of CAP was developed based on a CAP-specific aptamer. The CAP-specific aptamer was hybridized with a complementary short chain modified with fluorescent and quenching groups at both ends. Upon addition of CAP, the aptamer would be separated from its complementary probe and bind with CAP. The fluorescent and quenching groups labeled on the complementary probe were separated by exonuclease I (<b>Exo I</b>) digestion to release a fluorescent signal. Meanwhile, Exo I also digested the aptamer, and CAP was released from the complex, triggering the determination signal amplification based on CAP recycling. Factors (i.e., NaCl concentration and Exo I digestion time) that would affect the determination performance of this sensor system were optimized. Under the optimized conditions, the developed sensor system exhibited high determination sensitivity toward CAP with a limit of detection of 0.08 ng/mL (linear range from 0.1 to 5 ng/mL). Moreover, this sensor system was applied to determine CAP in real spiked milk, and the recovery rates ranged from 97.2 to 100.0%. Meanwhile, this sensor system also suggested cost efficiency with a determination cost of only $0.29 per sample. Results presented here and the high specificity of the aptamer suggested by the previous work indicated the application potential of this developed aptamer-based sensor system for the determination of CAP residue in animal-sourced food.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"79 12","pages":"1757 - 1763"},"PeriodicalIF":1.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Several techniques are available to determine an analyte, but the most preferred technique is fluorescence-based sensing owing to its sensitivity and selectivity. One of the main components of fluorescent-based sensors is a filter that allows a specific range of wavelengths to pass through and reach the sensing element. Existing filters still lack certain aspects such as durability, interference, cost, integration with a sensing element, biocompatibility, etc. This research aims to design and develop a polyvinyl acetate (PVAc)-benzophenone-based emission filter by spin coating and integrating it with a complementary metal-oxide semiconductor imaging sensor for use as a portable biochemical sensing device. In addition, the fabricated filter is intended to allow the conjugation of recognition elements necessary for biosensing using plasma oxidation. In this study, PVAc and benzophenone concentrations were optimized to fabricate an emission filter (EF) with precise thickness and uniformity. Subsequently, the fabricated EF underwent analysis using a single-beam UV-Vis spectrophotometer. Additionally, plasma treatment was administered to the EF, enabling further examination using fluorescein isothiocyanate dye under a fluorescent microscope. These examinations collectively suggest that the prepared filter possesses dual properties, i.e., it not only serves as an emission filter but also facilitates surface modification through plasma oxidation for the conjugation of chemical groups that can selectively bind to target analytes for biosensing purposes.
{"title":"Optimizing Fabrication Method and Surface Modification of Polyvinyl Acetate-Benzophenone Emission Filters for Complementary Metal-Oxide-Semiconductor Imager Chips towards Biosensing Applications","authors":"Narayan Yadav, Pooja Kumari, Snehlata Yadav, Sushmitha Paulraj, Parul Chaurasia, Aftab Anwar, Sanjeev Kumar Mahto","doi":"10.1134/S1061934824701363","DOIUrl":"10.1134/S1061934824701363","url":null,"abstract":"<p>Several techniques are available to determine an analyte, but the most preferred technique is fluorescence-based sensing owing to its sensitivity and selectivity. One of the main components of fluorescent-based sensors is a filter that allows a specific range of wavelengths to pass through and reach the sensing element. Existing filters still lack certain aspects such as durability, interference, cost, integration with a sensing element, biocompatibility, etc. This research aims to design and develop a polyvinyl acetate (<b>PVAc</b>)-benzophenone-based emission filter by spin coating and integrating it with a complementary metal-oxide semiconductor imaging sensor for use as a portable biochemical sensing device. In addition, the fabricated filter is intended to allow the conjugation of recognition elements necessary for biosensing using plasma oxidation. In this study, PVAc and benzophenone concentrations were optimized to fabricate an emission filter (<b>EF</b>) with precise thickness and uniformity. Subsequently, the fabricated EF underwent analysis using a single-beam UV-Vis spectrophotometer. Additionally, plasma treatment was administered to the EF, enabling further examination using fluorescein isothiocyanate dye under a fluorescent microscope. These examinations collectively suggest that the prepared filter possesses dual properties, i.e., it not only serves as an emission filter but also facilitates surface modification through plasma oxidation for the conjugation of chemical groups that can selectively bind to target analytes for biosensing purposes.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"79 12","pages":"1790 - 1799"},"PeriodicalIF":1.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-14DOI: 10.1134/S1061934824120013
A. V. Gorbovskaia, A. A. Timichev, A. V. Chernobrovkina, A. S. Uzhel, O. A. Shpigun
{"title":"Erratum to: Novel Highly Hydrophilic Resins with Attached Polymer Layers for Liquid Chromatography","authors":"A. V. Gorbovskaia, A. A. Timichev, A. V. Chernobrovkina, A. S. Uzhel, O. A. Shpigun","doi":"10.1134/S1061934824120013","DOIUrl":"10.1134/S1061934824120013","url":null,"abstract":"","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"79 12","pages":"1898 - 1898"},"PeriodicalIF":1.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-14DOI: 10.1134/S1061934824701272
Mohammad Rezaee
A new, fast, and environmentally friendly method based on ultrasound-assisted extraction combined with dispersive liquid-liquid microextraction using a low-density solvent was developed and optimized for assessing the levels of fenvalerate in strawberry samples. Variables affecting the performance of both steps were thoroughly investigated. Under the optimum conditions, relative recoveries for strawberry samples were in the range of 88.0–98.0%. The calibration graph was linear in the range of 10–1000 µg/kg, and the limits of detection and quantification were 3 and 10 µg/kg, respectively. The relative standard deviation for 40.0 µg/kg of fenvalerate in strawberry samples was 8.1% (n = 5). The obtained results show that the proposed method is a fast and simple method for the determination of fenvalerate in strawberry samples.
{"title":"Ultrasound-Assisted Extraction Combined with Dispersive Liquid-Liquid Microextraction Using Low-Density Solvent—a Novel Approach to Determine Fenvalerate in Strawberry Samples","authors":"Mohammad Rezaee","doi":"10.1134/S1061934824701272","DOIUrl":"10.1134/S1061934824701272","url":null,"abstract":"<p>A new, fast, and environmentally friendly method based on ultrasound-assisted extraction combined with dispersive liquid-liquid microextraction using a low-density solvent was developed and optimized for assessing the levels of fenvalerate in strawberry samples. Variables affecting the performance of both steps were thoroughly investigated. Under the optimum conditions, relative recoveries for strawberry samples were in the range of 88.0–98.0%. The calibration graph was linear in the range of 10–1000 µg/kg, and the limits of detection and quantification were 3 and 10 µg/kg, respectively. The relative standard deviation for 40.0 µg/kg of fenvalerate in strawberry samples was 8.1% (<i>n</i> = 5). The obtained results show that the proposed method is a fast and simple method for the determination of fenvalerate in strawberry samples.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"79 12","pages":"1710 - 1716"},"PeriodicalIF":1.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-14DOI: 10.1134/S1061934824701351
V. R. Khabibullin, N. N. Shevchenko, M. A. Proskurnin
Polymer micro- and nanoparticles, commonly known as microplastics, are significant environmental pollutants. Even low concentrations of microplastics can affect ecosystems and human health, underscoring the urgent need in detecting these particles in low concentrations while simultaneously assessing the physicochemical parameters of the studied systems. Thermal lens spectrometry (TLS) has effectively detected polystyrene particles measuring 65 and 80 nm within a concentration range of 0.0005–0.15 mg/L in their aqueous dispersions. The comprehensive TLS data also facilitated the evaluation of the thermal diffusivity of these solutions. An increase in the concentration of polystyrene nanoparticles in water results in a nonlinear change in thermal diffusivity.
{"title":"Determination of Polystyrene Nanoparticles in Aqueous Solutions by Dual-Beam Thermal Lens Spectrometry","authors":"V. R. Khabibullin, N. N. Shevchenko, M. A. Proskurnin","doi":"10.1134/S1061934824701351","DOIUrl":"10.1134/S1061934824701351","url":null,"abstract":"<div><p>Polymer micro- and nanoparticles, commonly known as microplastics, are significant environmental pollutants. Even low concentrations of microplastics can affect ecosystems and human health, underscoring the urgent need in detecting these particles in low concentrations while simultaneously assessing the physicochemical parameters of the studied systems. Thermal lens spectrometry (TLS) has effectively detected polystyrene particles measuring 65 and 80 nm within a concentration range of 0.0005–0.15 mg/L in their aqueous dispersions. The comprehensive TLS data also facilitated the evaluation of the thermal diffusivity of these solutions. An increase in the concentration of polystyrene nanoparticles in water results in a nonlinear change in thermal diffusivity.</p></div>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"79 12","pages":"1779 - 1789"},"PeriodicalIF":1.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A micelle-mediated cloud point extraction for carbendazim determination in vegetable and environmental samples using UV-Vis spectrophotometry was developed. Parameters of the proposed method (i.e., pH, extraction time, concentration of surfactant, concentration of ferricyanide, concentration of ferric chloride, extraction temperature, and sample volume) were optimized by UV-Vis spectrophotometry. In this study, the developed method was based on the oxidation reaction of carbendazim by the ferric ion (Fe3+) under acidic conditions, combined with potassium ferricyanide (K3[Fe(CN)6]) to form potassium hexacyanoferrate complexes or the Turnbull’s blue product quantitatively. The final products were solubilized into surfactant micelles, and carbendazim was determined by UV-Vis spectrophotometry at 685 nm after cloud point extraction. The analytical performance of the proposed method was obtained under optimal conditions. The limits of detection and quantification were found to be 0.12 and 0.41 mg/L, respectively. Precision factors were expressed as intra-day (1.6%) and inter-day (7.2%) variations over three replications. Furthermore, the proposed method was applied to the determination of carbendazim in environmental samples such as tap water, underground water, surface water, and in vegetable samples such as garlic, shallot, dried chili, celery, and peanuts. Recovery was obtained in the range of 83–113%.
{"title":"Cloud Point Extraction and Spectrophotometric Determination of Carbendazim Fungicide in Vegetable and Environmental Samples","authors":"Naruesorn Samanpong, Yuthapong Udnan, Ajchara lmkum Putkum, Wipharat Chuachuad Chaiyasith, Richmond Jerry Ampiah-Bonney","doi":"10.1134/S1061934824701260","DOIUrl":"10.1134/S1061934824701260","url":null,"abstract":"<p>A micelle-mediated cloud point extraction for carbendazim determination in vegetable and environmental samples using UV-Vis spectrophotometry was developed. Parameters of the proposed method (i.e., pH, extraction time, concentration of surfactant, concentration of ferricyanide, concentration of ferric chloride, extraction temperature, and sample volume) were optimized by UV-Vis spectrophotometry. In this study, the developed method was based on the oxidation reaction of carbendazim by the ferric ion (Fe<sup>3+</sup>) under acidic conditions, combined with potassium ferricyanide (K<sub>3</sub>[Fe(CN)<sub>6</sub>]) to form potassium hexacyanoferrate complexes or the Turnbull’s blue product quantitatively. The final products were solubilized into surfactant micelles, and carbendazim was determined by UV-Vis spectrophotometry at 685 nm after cloud point extraction. The analytical performance of the proposed method was obtained under optimal conditions. The limits of detection and quantification were found to be 0.12 and 0.41 mg/L, respectively. Precision factors were expressed as intra-day (1.6%) and inter-day (7.2%) variations over three replications. Furthermore, the proposed method was applied to the determination of carbendazim in environmental samples such as tap water, underground water, surface water, and in vegetable samples such as garlic, shallot, dried chili, celery, and peanuts. Recovery was obtained in the range of 83–113%.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"79 12","pages":"1701 - 1709"},"PeriodicalIF":1.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-14DOI: 10.1134/S1061934824701296
K. O. Kazimirova, S. N. Shtykov
Magnetic nanoparticles of magnetite (MNPs) with surfaces modified by biocompatible cationic polyelectrolytes polyethyleneimine (PEI) and chitosan (CS) are synthesized by the chemical coprecipitation method. The magnetic nanoparticles were characterized by transmission electron microscopy and measurements of zeta (ζ) potential. The initial MNPs exhibit shapes close to spherical, with an average size of 10 ± 3 nm. The immobilization of a polyelectrolyte on the surface of MNPs results in the formation of aggregates with an interconnected porous network (shell) around individual particles with average sizes of 12 ± 2 nm for Fe3O4@PEI and 15 ± 2 nm for Fe3O4@CS, respectively. The effect of various experimental parameters, such as pH, extraction time, amount of adsorbent, and initial concentration of the dye, on the adsorption and desorption of food azo dyes Allura Red AC (E129) and Black Brilliant BN (E151) has been thoroughly investigated. The results demonstrate that, under optimal conditions, the recovery of these dyes from aqueous solutions reaches 96–100%, at a concentration factor of 2.7 × 103 and adsorption capacities of 56 and 94 mg/g for Fe3O4@PEI, and 46 and 69 mg/g for Fe3O4@CS for E129 and E151, respectively. A comparison of the adsorption isotherms and kinetics of the process indicates that the Langmuir model and pseudo-second-order kinetics are preferable for describing the dye adsorption process. In the acidic and neutral pH ranges, electrostatic interactions primarily drive the adsorption process, while in the alkaline region, hydrogen bonding and hydrophobic interactions also play significant roles. The proposed adsorbents can be utilized for both the adsorption and preconcentration of the dyes in chemical analysis and the treatment of wastewaters for the dye removal. The preferred material for use is polyethyleneimine-modified nanomagnetite, which enables the adsorption and preconcentration of dyes across a wide pH range of 6–9.
采用化学共沉淀法合成了表面由生物相容性阳离子聚电解质聚乙烯亚胺(PEI)和壳聚糖(CS)修饰的磁铁矿磁性纳米颗粒(MNPs)。通过透射电子显微镜和ζ (ζ)电位测量对磁性纳米颗粒进行了表征。初始MNPs的形状接近球形,平均尺寸为10±3 nm。将聚电解质固定在MNPs表面,在单个颗粒周围形成具有相互连接的多孔网络(壳)的聚集体,其平均尺寸分别为Fe3O4@PEI(12±2 nm)和Fe3O4@CS(15±2 nm)。研究了pH、萃取时间、吸附剂用量、染料初始浓度等实验参数对食品偶氮染料Allura Red AC (E129)和Black Brilliant BN (E151)吸附和解吸的影响。结果表明,在最佳条件下,E129和E151对Fe3O4@PEI的吸附量分别为56和94 mg/g, Fe3O4@CS的吸附量分别为46和69 mg/g,对E129和E151的回收率为96 ~ 100%。吸附等温线和动力学比较表明,Langmuir模型和拟二级动力学更适合于描述染料吸附过程。在酸性和中性pH范围内,静电相互作用主要驱动吸附过程,而在碱性区域,氢键和疏水相互作用也起重要作用。所述吸附剂既可用于化学分析中染料的吸附和预富集,也可用于废水的脱色处理。首选材料是聚乙烯亚胺修饰的纳米磁铁矿,它可以在6-9的宽pH范围内吸附和预富集染料。
{"title":"Adsorption and Preconcentration of Anionic Azo Dyes on Nanomagnetite Modified with Cationic Polyelectrolytes","authors":"K. O. Kazimirova, S. N. Shtykov","doi":"10.1134/S1061934824701296","DOIUrl":"10.1134/S1061934824701296","url":null,"abstract":"<p>Magnetic nanoparticles of magnetite (MNPs) with surfaces modified by biocompatible cationic polyelectrolytes polyethyleneimine (PEI) and chitosan (CS) are synthesized by the chemical coprecipitation method. The magnetic nanoparticles were characterized by transmission electron microscopy and measurements of zeta (ζ) potential. The initial MNPs exhibit shapes close to spherical, with an average size of 10 ± 3 nm. The immobilization of a polyelectrolyte on the surface of MNPs results in the formation of aggregates with an interconnected porous network (shell) around individual particles with average sizes of 12 ± 2 nm for Fe<sub>3</sub>O<sub>4</sub>@PEI and 15 ± 2 nm for Fe<sub>3</sub>O<sub>4</sub>@CS, respectively. The effect of various experimental parameters, such as pH, extraction time, amount of adsorbent, and initial concentration of the dye, on the adsorption and desorption of food azo dyes Allura Red AC (E129) and Black Brilliant BN (E151) has been thoroughly investigated. The results demonstrate that, under optimal conditions, the recovery of these dyes from aqueous solutions reaches 96–100%, at a concentration factor of 2.7 × 10<sup>3</sup> and adsorption capacities of 56 and 94 mg/g for Fe<sub>3</sub>O<sub>4</sub>@PEI, and 46 and 69 mg/g for Fe<sub>3</sub>O<sub>4</sub>@CS for E129 and E151, respectively. A comparison of the adsorption isotherms and kinetics of the process indicates that the Langmuir model and pseudo-second-order kinetics are preferable for describing the dye adsorption process. In the acidic and neutral pH ranges, electrostatic interactions primarily drive the adsorption process, while in the alkaline region, hydrogen bonding and hydrophobic interactions also play significant roles. The proposed adsorbents can be utilized for both the adsorption and preconcentration of the dyes in chemical analysis and the treatment of wastewaters for the dye removal. The preferred material for use is polyethyleneimine-modified nanomagnetite, which enables the adsorption and preconcentration of dyes across a wide pH range of 6–9.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"79 12","pages":"1724 - 1732"},"PeriodicalIF":1.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-14DOI: 10.1134/S1061934824701375
P. A. Godunov, A. Yu. Shishov, A. V. Bulatov
A rapid and an environmentally friendly method is developed for the microextraction of furan derivatives from transformer oil for their determination by high-performance liquid chromatography with ultraviolet spectrophotometric detection. Various hydrophilic eutectic solvents were studied as extractants to implement vortex-assisted dispersive liquid–liquid microextraction. The results indicate that a three-component eutectic solvent composed of choline chloride, acetic acid, and water achieves the highest recoveries, ranging from 85 to 96%. The rapid spontaneous phase separation eliminated the need in centrifugation. Limits of detection (3σ) were achieved between 1 and 5 µg/L.
{"title":"Microextraction-Chromatographic Determination of Furan Derivatives in Transformer Oil","authors":"P. A. Godunov, A. Yu. Shishov, A. V. Bulatov","doi":"10.1134/S1061934824701375","DOIUrl":"10.1134/S1061934824701375","url":null,"abstract":"<p>A rapid and an environmentally friendly method is developed for the microextraction of furan derivatives from transformer oil for their determination by high-performance liquid chromatography with ultraviolet spectrophotometric detection. Various hydrophilic eutectic solvents were studied as extractants to implement vortex-assisted dispersive liquid–liquid microextraction. The results indicate that a three-component eutectic solvent composed of choline chloride, acetic acid, and water achieves the highest recoveries, ranging from 85 to 96%. The rapid spontaneous phase separation eliminated the need in centrifugation. Limits of detection (3σ) were achieved between 1 and 5 µg/L.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"79 12","pages":"1800 - 1807"},"PeriodicalIF":1.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-14DOI: 10.1134/S1061934824701466
A. V. Porfireva, Z. F. Khusnutdinova, G. A. Evtyugin
A voltammetric DNA sensor has been developed to detect DNA damage from calf thymus by measuring changes in redox signals in cyclic voltammograms of poly(Acridine Orange) (PAO) coatings. This polymer was synthesized on a carbon printed electrode from deep eutectic solvents—reline and glyceline—and a phosphate buffer solution. The optimal conditions for immobilizing DNA on each of the polymer coatings have been found. The effect of the polymerization medium on the electrochemical characteristics of the polyacridine dye layer and the polymer sensitivity to the thermal and oxidative damage of DNA has been identified. With the optimal composition of the surface layer the DNA sensor based on poly(Acridine Orange) synthesized from aqueous media (PAO1) reliably detected only chemical DNA oxidation. In contrast, the PAO synthesized from reline (PAO2) and glyceline (PAO3) demonstrated increased sensitivity to the incorporation of calf thymus DNA, which allows to discriminate between native, thermally denatured, and chemically oxidized DNA.
通过测量聚(吖啶橙)(PAO)涂层循环伏安图中氧化还原信号的变化,开发出了一种伏安 DNA 传感器,用于检测小牛胸腺的 DNA 损伤。这种聚合物是用深共晶溶剂--line 和甘氨酸--以及磷酸盐缓冲溶液在碳印刷电极上合成的。在每种聚合物涂层上固定 DNA 的最佳条件已经找到。聚合介质对聚吖啶染料层的电化学特性以及聚合物对 DNA 的热损伤和氧化损伤的敏感性的影响已经确定。在表层成分最佳的情况下,基于水介质合成的聚(吖啶橙)DNA 传感器(PAO1)只能可靠地检测 DNA 的化学氧化。与此相反,由reline(PAO2)和glyceline(PAO3)合成的聚吖啶橙(PAO)对加入小牛胸腺 DNA 的灵敏度更高,可以区分原生 DNA、热变性 DNA 和化学氧化 DNA。
{"title":"Voltammetric DNA Sensors for the DNA Damage Detection Using Poly(Acridine Orange) Coatings Deposited from Reline and Glyceline","authors":"A. V. Porfireva, Z. F. Khusnutdinova, G. A. Evtyugin","doi":"10.1134/S1061934824701466","DOIUrl":"10.1134/S1061934824701466","url":null,"abstract":"<p>A voltammetric DNA sensor has been developed to detect DNA damage from calf thymus by measuring changes in redox signals in cyclic voltammograms of poly(Acridine Orange) (PAO) coatings. This polymer was synthesized on a carbon printed electrode from deep eutectic solvents—reline and glyceline—and a phosphate buffer solution. The optimal conditions for immobilizing DNA on each of the polymer coatings have been found. The effect of the polymerization medium on the electrochemical characteristics of the polyacridine dye layer and the polymer sensitivity to the thermal and oxidative damage of DNA has been identified. With the optimal composition of the surface layer the DNA sensor based on poly(Acridine Orange) synthesized from aqueous media (PAO1) reliably detected only chemical DNA oxidation. In contrast, the PAO synthesized from reline (PAO2) and glyceline (PAO3) demonstrated increased sensitivity to the incorporation of calf thymus DNA, which allows to discriminate between native, thermally denatured, and chemically oxidized DNA.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"79 12","pages":"1887 - 1897"},"PeriodicalIF":1.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-14DOI: 10.1134/S1061934824701259
A. S. Zavalyueva, S. I. Karpov, V. F. Selemenev
The application of nanostructured adsorbents in sample preparation (extraction and preconcentration) of multicomponent mixtures in the solid-phase extraction of organic compounds is gaining significant interest. This study examines the adsorption properties of ordered mesoporous analogues of SBA-15, synthesized in the presence of quercetin as a potential adsorbate. The work explores the specific features of the adsorption-based recovery and preconcentration of quercetin, dihydroquercetin, naringin, and rutin under dynamic conditions from acetonitrile solutions using the breakthrough curve method. The use of the generalized optimization criterion of adsorption preconcentration CE, within the framework of the Venitsianov–Tsysin model of dynamic adsorption preconcentration, taking into account the limiting (mixed-diffusion) stage of adsorption kinetics, enabled the evaluation of the efficiency of the adsorption preconcentration of flavonoids on the studied adsorbents. The results demonstrate that the use of ordered silica synthesized in the presence of a potential adsorbate significantly enhances concentration efficiency compared to nonstructured silicas or unmodified SBA-15 analogue samples.
{"title":"Adsorption Preconcentration of Flavonoids on Ordered Mesoporous Silica Synthesized in the Presence of a Potential Adsorbate","authors":"A. S. Zavalyueva, S. I. Karpov, V. F. Selemenev","doi":"10.1134/S1061934824701259","DOIUrl":"10.1134/S1061934824701259","url":null,"abstract":"<p>The application of nanostructured adsorbents in sample preparation (extraction and preconcentration) of multicomponent mixtures in the solid-phase extraction of organic compounds is gaining significant interest. This study examines the adsorption properties of ordered mesoporous analogues of SBA-15, synthesized in the presence of quercetin as a potential adsorbate. The work explores the specific features of the adsorption-based recovery and preconcentration of quercetin, dihydroquercetin, naringin, and rutin under dynamic conditions from acetonitrile solutions using the breakthrough curve method. The use of the generalized optimization criterion of adsorption preconcentration <i>CE</i>, within the framework of the Venitsianov–Tsysin model of dynamic adsorption preconcentration, taking into account the limiting (mixed-diffusion) stage of adsorption kinetics, enabled the evaluation of the efficiency of the adsorption preconcentration of flavonoids on the studied adsorbents. The results demonstrate that the use of ordered silica synthesized in the presence of a potential adsorbate significantly enhances concentration efficiency compared to nonstructured silicas or unmodified SBA-15 analogue samples.</p>","PeriodicalId":606,"journal":{"name":"Journal of Analytical Chemistry","volume":"79 12","pages":"1694 - 1700"},"PeriodicalIF":1.0,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}