Pub Date : 2019-08-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.04.014
Shuwei Gong, Aifeng Liu, Mubin Wei, J. He
Knee osteoarthritis (KOA) is the main cause of knee pain and disability in the elderly. KOA is a complex disease that is controlled by a variety of pathogenic factors, including general physical factors such as age, gender, obesity, family history, and mechanical factors, and unfavorable mechanical factors to the knee joint, such as trauma, sports injuries, and daily routines. The prevalence of KOA is related to genetic factors. Currently, conservative treatment has limited effects to this disease, and knee joint replacement is usually associated with many complications. Mesenchymal stem cells(MSCs) were first discovered in the bone marrow and subsequently found in peripheral blood, cord blood, skeletal muscle, heart and adipose tissue, which have high plasticity and pluripotency. The discovery of MSCs provides a new method for the treatment of KOA. Due to its powerful cartilage repair and regeneration function, researchers have conducted a large number of experimental studies on the efficacy, safety and mechanism of MSCs in the treatment of KOA in recent years. In this paper, the above studies presented were systematically reviewed. Key words: Mesenchymal stem cells; Knee osteoarthritis; Application
{"title":"Research progress in the application of mesenchymal stem cells in the treatment of knee osteoarthritis","authors":"Shuwei Gong, Aifeng Liu, Mubin Wei, J. He","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.04.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.04.014","url":null,"abstract":"Knee osteoarthritis (KOA) is the main cause of knee pain and disability in the elderly. KOA is a complex disease that is controlled by a variety of pathogenic factors, including general physical factors such as age, gender, obesity, family history, and mechanical factors, and unfavorable mechanical factors to the knee joint, such as trauma, sports injuries, and daily routines. The prevalence of KOA is related to genetic factors. Currently, conservative treatment has limited effects to this disease, and knee joint replacement is usually associated with many complications. Mesenchymal stem cells(MSCs) were first discovered in the bone marrow and subsequently found in peripheral blood, cord blood, skeletal muscle, heart and adipose tissue, which have high plasticity and pluripotency. The discovery of MSCs provides a new method for the treatment of KOA. Due to its powerful cartilage repair and regeneration function, researchers have conducted a large number of experimental studies on the efficacy, safety and mechanism of MSCs in the treatment of KOA in recent years. In this paper, the above studies presented were systematically reviewed. \u0000 \u0000 \u0000Key words: \u0000Mesenchymal stem cells; Knee osteoarthritis; Application","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44828085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.04.007
J. Hou, Xi Peng, Peng Wang
Objective To compare and analyze the high risk factors of liver injury, and to explore the application of thermal texture maps (TTM) technology in the diagnosis and screening of liver injury. Methods A total of 3 582 medical examiners were selected as subjects, and their general clinical data, the related physical and chemical test results and TTM results were collected. The relationship between TTM results and general clinical data and related physical and chemical results was statistically analyzed. The general clinical data and physical and chemical results of subjects with TTM negative and TTM positive liver injury were compared from the sample value statistics and the sample group comparison. Results The differences in the degree of liver damage and the main related indicators between the two groups were statistically significant (all P<0.05), except for the body mass and low-density lipoprotein cholesterol level. When using aminotransferase as the standard, the diagnostic sensitivity of TTM for liver injury was 93.18%, the specificity was 68.34%, the positive predictive value was 54.34%, and the negative predictive value was 90.32%. Conclusions TTM examination can not only reflect the state of liver injury, but also reflect the degree of injury. TTM is an important index and necessary supplement for evaluating liver injury. Key words: Thermal texture maps; Liver; Function; Injury; Metabolism
{"title":"Application of thermal tomography imaging in detection of liver injury","authors":"J. Hou, Xi Peng, Peng Wang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.04.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.04.007","url":null,"abstract":"Objective \u0000To compare and analyze the high risk factors of liver injury, and to explore the application of thermal texture maps (TTM) technology in the diagnosis and screening of liver injury. \u0000 \u0000 \u0000Methods \u0000A total of 3 582 medical examiners were selected as subjects, and their general clinical data, the related physical and chemical test results and TTM results were collected. The relationship between TTM results and general clinical data and related physical and chemical results was statistically analyzed. The general clinical data and physical and chemical results of subjects with TTM negative and TTM positive liver injury were compared from the sample value statistics and the sample group comparison. \u0000 \u0000 \u0000Results \u0000The differences in the degree of liver damage and the main related indicators between the two groups were statistically significant (all P<0.05), except for the body mass and low-density lipoprotein cholesterol level. When using aminotransferase as the standard, the diagnostic sensitivity of TTM for liver injury was 93.18%, the specificity was 68.34%, the positive predictive value was 54.34%, and the negative predictive value was 90.32%. \u0000 \u0000 \u0000Conclusions \u0000TTM examination can not only reflect the state of liver injury, but also reflect the degree of injury. TTM is an important index and necessary supplement for evaluating liver injury. \u0000 \u0000 \u0000Key words: \u0000Thermal texture maps; Liver; Function; Injury; Metabolism","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45552360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To study the clinical efficacy of Nd:YAP laser in the treatment of acute localized pericardial periodontitis. Methods A total of 120 patients with acute localized pericardial periodontitis were randomly divided into 3 groups, including Nd:YAP laser group, minocycline group and control group (iodine glycerol group). All patients were underwent pretreatment of intracrevicular washing with 3.0% hydrogen peroxide and normal saline alternately. After the pretreatment, the patients in the Nd:YAP laser group were given 3 min local Nb:YAP laser irradiation (1 time/d for 3 times), in the minocycline group were injected with minocycline hydrochloride ointment (1 time), and in the control group were treated with 2% iodine glycerol (1 time/d for 4 times) in gingival sulcus. One day after the treatment, the gingival index (GI), pain visual score (VAS), and opening degree of all patients were recorded, and the therapeutic effect was observed 5 days after the treatment. Results Compared with the minocycline group and the control group (iodine glycerol group), the GI value and VAS value of the Nd:YAP laser group decreased and the openmouthed size increased (all P 0.05). Conclusions The Nd:YAP laser treatment inacute wisdom tooth pericoronitis can significantly reduce pain and improve openmouthed size, and has a good clinical efficacy. Key words: Nd:YAG laser; Minocycline hydrochloride ointment; Wisdom tooth pericoronitis
{"title":"Clinical efficacy of Nd:YAP laser in the treatment of acute wisdom tooth pericoronitis","authors":"Wanning Xu, Xin Huang, Zhaosong Meng, Dongwang Zhu","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.04.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.04.010","url":null,"abstract":"Objective \u0000To study the clinical efficacy of Nd:YAP laser in the treatment of acute localized pericardial periodontitis. \u0000 \u0000 \u0000Methods \u0000A total of 120 patients with acute localized pericardial periodontitis were randomly divided into 3 groups, including Nd:YAP laser group, minocycline group and control group (iodine glycerol group). All patients were underwent pretreatment of intracrevicular washing with 3.0% hydrogen peroxide and normal saline alternately. After the pretreatment, the patients in the Nd:YAP laser group were given 3 min local Nb:YAP laser irradiation (1 time/d for 3 times), in the minocycline group were injected with minocycline hydrochloride ointment (1 time), and in the control group were treated with 2% iodine glycerol (1 time/d for 4 times) in gingival sulcus. One day after the treatment, the gingival index (GI), pain visual score (VAS), and opening degree of all patients were recorded, and the therapeutic effect was observed 5 days after the treatment. \u0000 \u0000 \u0000Results \u0000Compared with the minocycline group and the control group (iodine glycerol group), the GI value and VAS value of the Nd:YAP laser group decreased and the openmouthed size increased (all P 0.05). \u0000 \u0000 \u0000Conclusions \u0000The Nd:YAP laser treatment inacute wisdom tooth pericoronitis can significantly reduce pain and improve openmouthed size, and has a good clinical efficacy. \u0000 \u0000 \u0000Key words: \u0000Nd:YAG laser; Minocycline hydrochloride ointment; Wisdom tooth pericoronitis","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45832278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.04.011
Yang Zhao, Xiaojie Wang, Lei Ma, Dangguo Shao, Y. Xiang, Xin Xiong, L. Zhang
Objective To predict the 5-year survival of patients with non-small cell lung cancer (NSCLC) by machine learning, and to improve the prediction efficiency and prediction accuracy. Methods The experiments were performed using NSCLC data from the SEER database. According to the imbalance of patient data, the Borderline-SMOTE method was used for data sampling. The perturbation-based feature selection (PFS) method and decision tree (DT) algorithm were used to screen the features and construct the postoperative survival prediction model. Results The patient data was balanced, and seven prognostic variables were screened, including primary site, stage group, surgical primary site, international classification of diseases, race and grade. Compared with LASSO, Tree-based, PFS-SVM and PFS-kNN models, the model constructed using PFS-DT has the best predictive effect. Conclusions The patient survival prediction model based on PFS-DT can effectively improve the accuracy of postoperative survival prediction in patients with NSCLC, and can provide a reference for doctors to provide treatment and improve prognosis. Key words: Non-small cell lung cancer; Imbalance; Feature selection; Survival prediction
{"title":"Constructing non-small cell lung cancer survival prediction model based on Borderline-SMOTE and PFS","authors":"Yang Zhao, Xiaojie Wang, Lei Ma, Dangguo Shao, Y. Xiang, Xin Xiong, L. Zhang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.04.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.04.011","url":null,"abstract":"Objective \u0000To predict the 5-year survival of patients with non-small cell lung cancer (NSCLC) by machine learning, and to improve the prediction efficiency and prediction accuracy. \u0000 \u0000 \u0000Methods \u0000The experiments were performed using NSCLC data from the SEER database. According to the imbalance of patient data, the Borderline-SMOTE method was used for data sampling. The perturbation-based feature selection (PFS) method and decision tree (DT) algorithm were used to screen the features and construct the postoperative survival prediction model. \u0000 \u0000 \u0000Results \u0000The patient data was balanced, and seven prognostic variables were screened, including primary site, stage group, surgical primary site, international classification of diseases, race and grade. Compared with LASSO, Tree-based, PFS-SVM and PFS-kNN models, the model constructed using PFS-DT has the best predictive effect. \u0000 \u0000 \u0000Conclusions \u0000The patient survival prediction model based on PFS-DT can effectively improve the accuracy of postoperative survival prediction in patients with NSCLC, and can provide a reference for doctors to provide treatment and improve prognosis. \u0000 \u0000 \u0000Key words: \u0000Non-small cell lung cancer; Imbalance; Feature selection; Survival prediction","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44356089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.04.003
Xiangfeng Wu, Tao Wang, Tao Liu, Fu-yu Xu, Qian Zhang
Objective To investigate the effects and the mechanism of thrombospondin 4 (Thbs4) gene-edited bone marrow mesenchymal stem cells (BMSCs) transplantation on vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) in diabetic rats with hind limb ischemia. Methods Thirty Sprague-Dawley rats were randomly divided into the model group, BMSCs treatment group and Thbs4-BMSCs treatment group on average. After constructing the type Ⅱ diabetic rat model with hind limb ischemia, 100 μl normal saline, BMSCs suspension and Thbs4-BMSCs suspension (cell number: 2×106) were locally injected into the ischemic injury area of rats for the model group, BMSCs group and Thbs4-BMSCs group, respectively. The rats were sacrificed on the 14th day after stem cell transplantation, and the muscle tissues near the ischemic area were collected. The relative expression of VEGF and p-Smad2/3 protein was detected by Western Blot. The Ang-1 protein expression was detected by immunofluorescence staining. The levels of related genes were detected by qRT-PCR, and the von Willebrand Factor (vWF) protein expression was detected by immunohistochemistry staining. Results The relative expression levels of VEGF, Ang-1 and vWF protein in the Thbs4-BMSCs group were significantly higher than those in the model group and BMSCs group (VEGF protein: P<0.01 and P<0.05). The mRNA expression of VEGF and Ang-1 were significantly up-regulated, the differences were statistically significant(VEGF mRNA: all P<0.01; Ang-1: P<0.01 and P<0.05). The expression of p-Smad2/3 protein in the Thbs4-BMSCs group was significantly higher than that in the model group and the BMSCs treatment group (all P<0.01). The expression of p-Smad2/3 protein was significantly decreased after the addition of p-Smad2/3 pathway inhibitor, the differences were statistically significant (P<0.05). Conclusions Thbs4-BMSCs transplantation can effectively promote angiogenesis in diabetic rats with hind limb ischemia, and the effect of angiogenesis may be related to the activation of Smad2/3 signaling pathway. Key words: Diabetes mellitus; Hind limb ischemia; Mesenchymal stem cell transplantation; Thrombospondin 4; Angiogenesis
{"title":"Effect of Thbs4 gene editing BMSCs transplantation on VEGF and Ang-1 in diabetic hind limb ischemia rats and related mechanism","authors":"Xiangfeng Wu, Tao Wang, Tao Liu, Fu-yu Xu, Qian Zhang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.04.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.04.003","url":null,"abstract":"Objective \u0000To investigate the effects and the mechanism of thrombospondin 4 (Thbs4) gene-edited bone marrow mesenchymal stem cells (BMSCs) transplantation on vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) in diabetic rats with hind limb ischemia. \u0000 \u0000 \u0000Methods \u0000Thirty Sprague-Dawley rats were randomly divided into the model group, BMSCs treatment group and Thbs4-BMSCs treatment group on average. After constructing the type Ⅱ diabetic rat model with hind limb ischemia, 100 μl normal saline, BMSCs suspension and Thbs4-BMSCs suspension (cell number: 2×106) were locally injected into the ischemic injury area of rats for the model group, BMSCs group and Thbs4-BMSCs group, respectively. The rats were sacrificed on the 14th day after stem cell transplantation, and the muscle tissues near the ischemic area were collected. The relative expression of VEGF and p-Smad2/3 protein was detected by Western Blot. The Ang-1 protein expression was detected by immunofluorescence staining. The levels of related genes were detected by qRT-PCR, and the von Willebrand Factor (vWF) protein expression was detected by immunohistochemistry staining. \u0000 \u0000 \u0000Results \u0000The relative expression levels of VEGF, Ang-1 and vWF protein in the Thbs4-BMSCs group were significantly higher than those in the model group and BMSCs group (VEGF protein: P<0.01 and P<0.05). The mRNA expression of VEGF and Ang-1 were significantly up-regulated, the differences were statistically significant(VEGF mRNA: all P<0.01; Ang-1: P<0.01 and P<0.05). The expression of p-Smad2/3 protein in the Thbs4-BMSCs group was significantly higher than that in the model group and the BMSCs treatment group (all P<0.01). The expression of p-Smad2/3 protein was significantly decreased after the addition of p-Smad2/3 pathway inhibitor, the differences were statistically significant (P<0.05). \u0000 \u0000 \u0000Conclusions \u0000Thbs4-BMSCs transplantation can effectively promote angiogenesis in diabetic rats with hind limb ischemia, and the effect of angiogenesis may be related to the activation of Smad2/3 signaling pathway. \u0000 \u0000 \u0000Key words: \u0000Diabetes mellitus; Hind limb ischemia; Mesenchymal stem cell transplantation; Thrombospondin 4; Angiogenesis","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45968460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.04.009
Liming Chen, Bo-Ya Zhang, Yuetong Hui, Tao Yang, H. Sha, Huipeng Meng, Yanlong Zhang, Keqiang Wang, Lei Wang, Jian Xu
Objective To investigate the supernatant of umbilical cord-derived mesenchymal stem cells (UCMSCs) on tumor necrosis factor-α (TNF-α) and apoptosis protein caspase-3 in diabetic rats model with skin ulcer. Methods 45 Sprague-Dawley rats were randomly divided into control group (acute wounds group), phosphate buffered saline (PBS) group and UCMSCs supernatant group. The diabetic rat model was constructed by injecting with alloxan by tail vein and feeding with high-fat diet. Diabetic skin ulcer (DSU) rat model was constructed by scratching a wound and infusing suspension of Staphylococcus aureus. In the control group, the diabetic rats (n=15) were scratched to form a wound and treated by tail vein injection of 100 μl PBS. In the PBS group, DSU rats (n=15) were treated by tail vein injection of 100 μl PBS, and then 100 μl PBS was dropped at the ulcer site. In the UCMSCs supernatant group, freeze-dried powder of UCMSCs supernatant was dissolved in 200 μl PBS, 100 μl of which was injected into the tail vein of DSU rats (n=15), and other 100 μl was dropped at the ulcer site. After 5 days of the treatments, the levels of serum TNF-α were detected by radioimmunoassay method, and the expression of TNF-α and caspase-3 in the ulcer tissues of rats was detected by polymerase chain reaction and Western Blot. Results The levels of TNF-α in the PBS group [(35.9±3.7) μg/L] were significantly higher than that of the control group [(11.4±4.9) μg/L] and the UCMSCs group [(14.7±6.6) μg/L] (all P 0.05). Conclusions UCMSCs supernatant treatments can effectively down-regulate the expression of TNF-α and caspase-3 in ulcer tissue of DSU rats, and play an anti-inflammatory and anti-apoptotic effect. Key words: Diabetes mellitus; Skin ulcer; Umbilical cord derived-mesenchymal stem cells; Tumor necrosis factor-α; Caspase-3
{"title":"Effect of supernatant of mesenchymal stem cells on tumor necrosis factor-α and apoptosis protein caspase-3 in diabetic skin ulcer rat model","authors":"Liming Chen, Bo-Ya Zhang, Yuetong Hui, Tao Yang, H. Sha, Huipeng Meng, Yanlong Zhang, Keqiang Wang, Lei Wang, Jian Xu","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.04.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.04.009","url":null,"abstract":"Objective \u0000To investigate the supernatant of umbilical cord-derived mesenchymal stem cells (UCMSCs) on tumor necrosis factor-α (TNF-α) and apoptosis protein caspase-3 in diabetic rats model with skin ulcer. \u0000 \u0000 \u0000Methods \u000045 Sprague-Dawley rats were randomly divided into control group (acute wounds group), phosphate buffered saline (PBS) group and UCMSCs supernatant group. The diabetic rat model was constructed by injecting with alloxan by tail vein and feeding with high-fat diet. Diabetic skin ulcer (DSU) rat model was constructed by scratching a wound and infusing suspension of Staphylococcus aureus. In the control group, the diabetic rats (n=15) were scratched to form a wound and treated by tail vein injection of 100 μl PBS. In the PBS group, DSU rats (n=15) were treated by tail vein injection of 100 μl PBS, and then 100 μl PBS was dropped at the ulcer site. In the UCMSCs supernatant group, freeze-dried powder of UCMSCs supernatant was dissolved in 200 μl PBS, 100 μl of which was injected into the tail vein of DSU rats (n=15), and other 100 μl was dropped at the ulcer site. After 5 days of the treatments, the levels of serum TNF-α were detected by radioimmunoassay method, and the expression of TNF-α and caspase-3 in the ulcer tissues of rats was detected by polymerase chain reaction and Western Blot. \u0000 \u0000 \u0000Results \u0000The levels of TNF-α in the PBS group [(35.9±3.7) μg/L] were significantly higher than that of the control group [(11.4±4.9) μg/L] and the UCMSCs group [(14.7±6.6) μg/L] (all P 0.05). \u0000 \u0000 \u0000Conclusions \u0000UCMSCs supernatant treatments can effectively down-regulate the expression of TNF-α and caspase-3 in ulcer tissue of DSU rats, and play an anti-inflammatory and anti-apoptotic effect. \u0000 \u0000 \u0000Key words: \u0000Diabetes mellitus; Skin ulcer; Umbilical cord derived-mesenchymal stem cells; Tumor necrosis factor-α; Caspase-3","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42129285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.04.012
S. Ruan, Zhixin Zhao, Wen-yi Zhang, Kaijun Su, Xinxin Pang, F. Wang, Yulian Liu, Menghui Huo, Changxin Yan, L. Jiao
Objective To investigate effect of high dose irradiation on the performances of thermolumines- cence detectors (LiF:Mg, Cu, P). Methods The high-dose irradiated thermoluminescence detector was annealed by a thermoluminescence annealing furnace until the annealing was completed, and then the annealed thermoluminescent detector was irradiated 0.5Gy by 137Cs irradiator to verify the accuracy of the thermoluminescentdetector. Results The thermoluminescence detector after high-dose irradiation could not be completely annealed under the temperature condition of 240 °C, and it could be completely annealed at a high temperature as 400 ℃. After 0.5 Gy irradiation by 137Cs irradiator, the measurement results of the annealed thermoluminescence detector were significantly smaller, and the dose response and dispersion of the detector were also changed significantly. Conclusions After a more than 5 Gy irradiation, the crystal structure of the thermoluminescence detector has changed, and a high temperature peak above 240 ℃ has appeared, which leads to the failure to completely anneal at normal temperature. Therefore, the thermoluminescence detector can no longer be used for dosimetry after high-dose irradiation. Key words: Thermoluminescent detector; High dose; Annealing; Dose response
{"title":"Effect of high dose irradiation on thermoluminescence detector","authors":"S. Ruan, Zhixin Zhao, Wen-yi Zhang, Kaijun Su, Xinxin Pang, F. Wang, Yulian Liu, Menghui Huo, Changxin Yan, L. Jiao","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.04.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.04.012","url":null,"abstract":"Objective \u0000To investigate effect of high dose irradiation on the performances of thermolumines- cence detectors (LiF:Mg, Cu, P). \u0000 \u0000 \u0000Methods \u0000The high-dose irradiated thermoluminescence detector was annealed by a thermoluminescence annealing furnace until the annealing was completed, and then the annealed thermoluminescent detector was irradiated 0.5Gy by 137Cs irradiator to verify the accuracy of the thermoluminescentdetector. \u0000 \u0000 \u0000Results \u0000The thermoluminescence detector after high-dose irradiation could not be completely annealed under the temperature condition of 240 °C, and it could be completely annealed at a high temperature as 400 ℃. After 0.5 Gy irradiation by 137Cs irradiator, the measurement results of the annealed thermoluminescence detector were significantly smaller, and the dose response and dispersion of the detector were also changed significantly. \u0000 \u0000 \u0000Conclusions \u0000After a more than 5 Gy irradiation, the crystal structure of the thermoluminescence detector has changed, and a high temperature peak above 240 ℃ has appeared, which leads to the failure to completely anneal at normal temperature. Therefore, the thermoluminescence detector can no longer be used for dosimetry after high-dose irradiation. \u0000 \u0000 \u0000Key words: \u0000Thermoluminescent detector; High dose; Annealing; Dose response","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48251240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.04.013
Fei Tang, Linling Li, Mengying Wei
With the rapid development of neuroimaging technology and related data processing methods, multimodal neuroimaging has been widely used in research fields such as neuroscience and clinical diseases. In this paper, the current development of multimodal neuroimaging fusion algorithm and its application in the diagnosis and treatment of brain diseases were reviewed. The definitions, applications, and advantages of the three levels of multimodal neuroimaging fusion, i.e. early fusion, late fusion, and intermediate fusion, were introduced and analyzed. The commonly used multi-modal neuroimaging algorithm basing on signal source separation method and deep multi-modal learning was introduced. The application of multimodal neuroimaging technology in the diagnosis and treatment of severe brain diseases such as schizophrenia and Alzheimer's disease was further discussed. Finally, the existing challenges and future research directions of multimodal neuroimaging methods and applications were summarized. Key words: Multimodal fusion; Neuroimaging; Magnetic resonance imaging; Deep multimodal learning; Neurological diseases
{"title":"A review of multimodal neuroimaging fusion methods and their clinical applications to brain diseases","authors":"Fei Tang, Linling Li, Mengying Wei","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.04.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.04.013","url":null,"abstract":"With the rapid development of neuroimaging technology and related data processing methods, multimodal neuroimaging has been widely used in research fields such as neuroscience and clinical diseases. In this paper, the current development of multimodal neuroimaging fusion algorithm and its application in the diagnosis and treatment of brain diseases were reviewed. The definitions, applications, and advantages of the three levels of multimodal neuroimaging fusion, i.e. early fusion, late fusion, and intermediate fusion, were introduced and analyzed. The commonly used multi-modal neuroimaging algorithm basing on signal source separation method and deep multi-modal learning was introduced. The application of multimodal neuroimaging technology in the diagnosis and treatment of severe brain diseases such as schizophrenia and Alzheimer's disease was further discussed. Finally, the existing challenges and future research directions of multimodal neuroimaging methods and applications were summarized. \u0000 \u0000 \u0000Key words: \u0000Multimodal fusion; Neuroimaging; Magnetic resonance imaging; Deep multimodal learning; Neurological diseases","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47085561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.04.001
Yingxin Li
The fundamental goal of China's medical insurance is to build a multi-level social security system covering the whole people, urban and rural planning, clear rights and responsibilities, moderate protection, and sustainable development. It is required to unify the medical insurance system, achieve fairness in medical insurance, improve the degree of protection, and increase the protection project, so as to effectively meet the needs of the basic health care services of the national diversification and to comprehensively improve the national health level. It is help to reduce the incidence of diseases, improve the quality of life, reduce the expenditure on medical expenses, and regulate the health habits of the people by emphasizing the importance of active prevention in the medical insurance sector. That is consistent with the ultimate goal of the health system reform. Active health insurance based on the national big data platform for medical and healthcare management can implement effective health interventions through various medical and economic means, helping to block risk factors before disease formation, thereby extending the individual health cycle and achieving the purpose of maintaining health. It can be foreseen that with the implementation of active medical insurance, the awareness rate of major chronic diseases will increase, and the incidence of major chronic diseases and the number of patients will gradually decrease. Therefore, the economic burden of the national medical and health system will gradually be reduced, so as to meet the growing needs of people for health care. Key words: Health care big data; Health system reform; Medical insurance
{"title":"Medical insurance model based on the national big data platform for medical and healthcare management","authors":"Yingxin Li","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.04.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.04.001","url":null,"abstract":"The fundamental goal of China's medical insurance is to build a multi-level social security system covering the whole people, urban and rural planning, clear rights and responsibilities, moderate protection, and sustainable development. It is required to unify the medical insurance system, achieve fairness in medical insurance, improve the degree of protection, and increase the protection project, so as to effectively meet the needs of the basic health care services of the national diversification and to comprehensively improve the national health level. It is help to reduce the incidence of diseases, improve the quality of life, reduce the expenditure on medical expenses, and regulate the health habits of the people by emphasizing the importance of active prevention in the medical insurance sector. That is consistent with the ultimate goal of the health system reform. Active health insurance based on the national big data platform for medical and healthcare management can implement effective health interventions through various medical and economic means, helping to block risk factors before disease formation, thereby extending the individual health cycle and achieving the purpose of maintaining health. It can be foreseen that with the implementation of active medical insurance, the awareness rate of major chronic diseases will increase, and the incidence of major chronic diseases and the number of patients will gradually decrease. Therefore, the economic burden of the national medical and health system will gradually be reduced, so as to meet the growing needs of people for health care. \u0000 \u0000 \u0000Key words: \u0000Health care big data; Health system reform; Medical insurance","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46521812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To research the effect of alkylation of glycerol phosphate synthase(AGPS) in isoproterenol (ISO) induced rat cardiac hypertrophy. Methods The pathological cardiac hypertrophy rat model was constructed by ISO intraperitoneal injection. Twelve healthy Sprague-Dawley rats (120~150 g) were divided into ISO group and control group randomly. In the ISO group, rats were injected with ISO (3 mg/kg) per day for two consecutive weeks. In the control group, rats were injected with normal saline (3 mg/kg) per day for two consecutive weeks. Changes of left ventricular diastolic diameter, left ventricular posterior wall thickness, left ventricular ejection fraction, left ventricular short-axis shortening rate and left ventricular mass were detected by echocardiography. The cross-sectional area of myocardial cells in rats was measured by hematoxylin-eosin staining. The expression of hypertrophic factors [atrial natriuretic peptide (ANP), myosin light chain-2V (MLC-2V), α-myosin heavy chain (α-MHC)] and AGPS were detected by Western Blot and real-time quantitative PCR (qPCR). Results The results of echocardiography showed that the cardiac hypertrophy rat model was successfully constructed. The results of hematoxylin-eosin staining showed that the myocardial cross-sectional area in the ISO group was significantly larger than that of the control group. The Western Blot and qPCR results indicated that the relative expression of protein and mRNA of hypertrophic factor and AGPS in the ISO group were both up-regulated comparing with that of the control group, and the differences were statistical significance (all P<0.05). Conclusions The rat model of pathological cardiac hypertrophy with up-regulated AGPS expression was successfully constructed providing a theoretical basis for further study on the role of AGPS in pathogenesis of pathological cardiac hypertrophy. Key words: Cardiomyopathy, hypertrophic; Isoproterenol; Alkylglycerone phosphate synthase
{"title":"Role of the alkylglycerone phosphate synthase in isoproterenol-induced cardiac hypertrophy","authors":"Yijie Liu, Qiaoman Fei, Bingyan Cao, Manman Qiu, Huan Huang, Jiaxin Song, Bing Yang, Ling Zhang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.04.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.04.004","url":null,"abstract":"Objective \u0000To research the effect of alkylation of glycerol phosphate synthase(AGPS) in isoproterenol (ISO) induced rat cardiac hypertrophy. \u0000 \u0000 \u0000Methods \u0000The pathological cardiac hypertrophy rat model was constructed by ISO intraperitoneal injection. Twelve healthy Sprague-Dawley rats (120~150 g) were divided into ISO group and control group randomly. In the ISO group, rats were injected with ISO (3 mg/kg) per day for two consecutive weeks. In the control group, rats were injected with normal saline (3 mg/kg) per day for two consecutive weeks. Changes of left ventricular diastolic diameter, left ventricular posterior wall thickness, left ventricular ejection fraction, left ventricular short-axis shortening rate and left ventricular mass were detected by echocardiography. The cross-sectional area of myocardial cells in rats was measured by hematoxylin-eosin staining. The expression of hypertrophic factors [atrial natriuretic peptide (ANP), myosin light chain-2V (MLC-2V), α-myosin heavy chain (α-MHC)] and AGPS were detected by Western Blot and real-time quantitative PCR (qPCR). \u0000 \u0000 \u0000Results \u0000The results of echocardiography showed that the cardiac hypertrophy rat model was successfully constructed. The results of hematoxylin-eosin staining showed that the myocardial cross-sectional area in the ISO group was significantly larger than that of the control group. The Western Blot and qPCR results indicated that the relative expression of protein and mRNA of hypertrophic factor and AGPS in the ISO group were both up-regulated comparing with that of the control group, and the differences were statistical significance (all P<0.05). \u0000 \u0000 \u0000Conclusions \u0000The rat model of pathological cardiac hypertrophy with up-regulated AGPS expression was successfully constructed providing a theoretical basis for further study on the role of AGPS in pathogenesis of pathological cardiac hypertrophy. \u0000 \u0000 \u0000Key words: \u0000Cardiomyopathy, hypertrophic; Isoproterenol; Alkylglycerone phosphate synthase","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45375688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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