Pub Date : 2019-12-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.06.004
Wang‐gang Zhang
Objective To detect the expression of ACSL4 in human gastric cancer tissue and to analyze its clinical significance. Methods The bioinformatics method was used to analyze the mRNA level of ACSL4 in gastric cancer tissues and normal tissues, and to analyze the relationship between its expression and disease-free survival rate of gastric cancer patients. The clinical and pathological data of 62 patients with gastric cancer who underwent surgical treatment were retrospectively analyzed. Immunohistochemistry was used to detect the expression of ACSL4 protein in gastric cancer tissues and adjacent tissues, and to analyze its relationship with clinicopathological characteristics of gastric cancer patients. Results The results of bioinformatics analysis showed that the mRNA of ACSL4 was significantly overexpressed in gastric cancer tissues, and was significantly related to the disease-free survival rate of patients. Immunohistochemical results showed that ACSL4 was mainly cytoplasmic and highly expressed in gastric cancer tissues, while low or no expression in adjacent tissues. The protein expression level of ACSL4 was related to tumor size and pTNM (all P 0.05). Conclusions The expression of ACSL4 in gastric cancer tissues is abnormally increased, and it is related to tumor size and pTNM stage. The results of this study suggest that the expression of ACSL4 is related to the prognosis of patients. Key words: Gastric cancer; Immunohistochemistry; ACSL4; pTNM staging; Prognosis
{"title":"Expression of ACSL4 in gastric cancer tissues and its correlation with prognosis","authors":"Wang‐gang Zhang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.06.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.06.004","url":null,"abstract":"Objective \u0000To detect the expression of ACSL4 in human gastric cancer tissue and to analyze its clinical significance. \u0000 \u0000 \u0000Methods \u0000The bioinformatics method was used to analyze the mRNA level of ACSL4 in gastric cancer tissues and normal tissues, and to analyze the relationship between its expression and disease-free survival rate of gastric cancer patients. The clinical and pathological data of 62 patients with gastric cancer who underwent surgical treatment were retrospectively analyzed. Immunohistochemistry was used to detect the expression of ACSL4 protein in gastric cancer tissues and adjacent tissues, and to analyze its relationship with clinicopathological characteristics of gastric cancer patients. \u0000 \u0000 \u0000Results \u0000The results of bioinformatics analysis showed that the mRNA of ACSL4 was significantly overexpressed in gastric cancer tissues, and was significantly related to the disease-free survival rate of patients. Immunohistochemical results showed that ACSL4 was mainly cytoplasmic and highly expressed in gastric cancer tissues, while low or no expression in adjacent tissues. The protein expression level of ACSL4 was related to tumor size and pTNM (all P 0.05). \u0000 \u0000 \u0000Conclusions \u0000The expression of ACSL4 in gastric cancer tissues is abnormally increased, and it is related to tumor size and pTNM stage. The results of this study suggest that the expression of ACSL4 is related to the prognosis of patients. \u0000 \u0000 \u0000Key words: \u0000Gastric cancer; Immunohistochemistry; ACSL4; pTNM staging; Prognosis","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47996394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.06.015
Yan Rong-guo, Wei Ruofan, Shen Jiake
Clinically, routine urinalysis includes more than 10 items, such as urine protein, urinary pH, urinary bilirubin and so on. The above items are usually analyzed by dry chemical methods. The principle is that after the urine infiltrates the reagent strips, the color changes can be observed in each strip. The color depth is related to the component concentration, and the darker the color, the greater the measured component concentration, and vice versa. In this paper, four color discrimination methods in urinary dry chemical analysis were introduced, i.e. the method based on integrating sphere photoelectric conversion, the method based on charge-coupled device (CCD) image sensor, the method based on color sensor, and the method based on the combination of color sensor and fiber bundle. Besides, the four methods were compared and analyzed. Finally, the recent research progress in urinalysis was reviewed. Key words: Urine; Dry chemical method; Color; Urine reagent strip
{"title":"Research progress on color determination methods of reagent strips in routine urinalysis","authors":"Yan Rong-guo, Wei Ruofan, Shen Jiake","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.06.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.06.015","url":null,"abstract":"Clinically, routine urinalysis includes more than 10 items, such as urine protein, urinary pH, urinary bilirubin and so on. The above items are usually analyzed by dry chemical methods. The principle is that after the urine infiltrates the reagent strips, the color changes can be observed in each strip. The color depth is related to the component concentration, and the darker the color, the greater the measured component concentration, and vice versa. In this paper, four color discrimination methods in urinary dry chemical analysis were introduced, i.e. the method based on integrating sphere photoelectric conversion, the method based on charge-coupled device (CCD) image sensor, the method based on color sensor, and the method based on the combination of color sensor and fiber bundle. Besides, the four methods were compared and analyzed. Finally, the recent research progress in urinalysis was reviewed. \u0000 \u0000 \u0000Key words: \u0000Urine; Dry chemical method; Color; Urine reagent strip","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48470919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Skin is a kind of tissue that surrounds the surface of body, it is the first barrier for animals to resist mechanical, chemical and pathogenic microorganisms. Skin wound is one of the most common surgical diseases. The process of wound healing can be summarized as three stages: inflammation stage, fibrous tissue proliferation stage, and scar formation and repair stage. Incomplete repair of the wound leads to skin scarring, which causes the tissue to lose its normal structure and function, and seriously affects the aesthetic appearance. Traditional treatment methods can not restore the normal function of the skin and have obvious adverse reactions, which can not meet people's needs. Stem cell therapy, especially adipose derived stem cells (ADSCs) plays a essential rule in the process of wound healing making it a research hotspot in recent years. ADSCs can secrete a variety of growth factors during wound healing to reduce wound inflammatory response, promote wound regeneration epithelialization and vascular reconstruction, thereby promoting wound healing. In this paper, the wound healing process and its regulation mechanism were summarized, and the role of ADSCs in wound healing at home and abroad and its clinical application progress were reviewed. Key words: Adipose derived stem cells; Skin; Wound healing
{"title":"Research progress of adipose derived stem cells in wound healing","authors":"Yusu Wang, Wenhui Chu, Qi Wang, Chunyi Li, Quanmin Zhao","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.06.014","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.06.014","url":null,"abstract":"Skin is a kind of tissue that surrounds the surface of body, it is the first barrier for animals to resist mechanical, chemical and pathogenic microorganisms. Skin wound is one of the most common surgical diseases. The process of wound healing can be summarized as three stages: inflammation stage, fibrous tissue proliferation stage, and scar formation and repair stage. Incomplete repair of the wound leads to skin scarring, which causes the tissue to lose its normal structure and function, and seriously affects the aesthetic appearance. Traditional treatment methods can not restore the normal function of the skin and have obvious adverse reactions, which can not meet people's needs. Stem cell therapy, especially adipose derived stem cells (ADSCs) plays a essential rule in the process of wound healing making it a research hotspot in recent years. ADSCs can secrete a variety of growth factors during wound healing to reduce wound inflammatory response, promote wound regeneration epithelialization and vascular reconstruction, thereby promoting wound healing. In this paper, the wound healing process and its regulation mechanism were summarized, and the role of ADSCs in wound healing at home and abroad and its clinical application progress were reviewed. \u0000 \u0000 \u0000Key words: \u0000Adipose derived stem cells; Skin; Wound healing","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47228961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.06.007
Yingzi Cui, Ruonan Wang, Lihong Jiang
Objective To study the effects of ShenhongTongluo Granules on blood lipids, inflammatory related factors and oxidative stress-related indexes in rabbit atherosclerosis model, and to explore the mechanism of Shenhongtongluo Granules on atherosclerosis. Methods 70 healthy male New Zealand white rabbits were randomly divided into blank control group, model group, aspirin combined with simvastatin group, aspirin group, simvastatin group, Shenhongtongluo Granule prevention group, and Shenhongtongluo Granule treatment group. The rabbit atherosclerosis model was established by lipidforage diet combined with immune stimulation, and the blank group was fed with commonforage. The Shenhongtongluo Granule prevention group was given Shenhongtongluo Granule at the beginning of modeling, and the other intervention groups were given the corresponding drugs after the successful modeling. Before and after modeling, lipid changes were checked in each group, including total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), superoxide dismutase activity (SOD) and malondialdehyde (MDA) in the serum. The aorta of the model group was separated and oil red O staining was performed to observe the pathological and morphological changes of the aorta. Western blotting and reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of Nox4 and p22phox. Western blotting was used to detect the expression of TLR4 and NF-κB protein in aorta. Results Twelve weeks after modeling, atheromatous plaques were obviously observed on the aorta stained with oil red O, indicating successful atherosclerosis modeling. Compared with the blank control group, TC, TG, and LDL-C in the serum of the atherosclerosis model group were significantly increased (all P<0.05). Compared with the model group, the atherosclerotic plaques in the Shenhongtongluo Granule prevention and the treatment groups were significantly reduced, the levels of TC, TG, LDL-C in serum were decreased, and the levels of SOD were increased, and MDA levels were reduced (all P<0.05). The expressions of TLR4, NF-κB, Nox4, and p22phox in the aorta of Shenhongtongluo Granule prevention group were significantly reduced (all P<0.05). Conclusions Shenhongtongluo Granules can inhibit the formation of atheromatous plaques, have a good effect on regulating blood lipids, and can improve the ability of anti-oxidative stress, and have a good preventive and therapeutic effect. Key words: Atherosclerosis; Oxidative stress; Inflammatory response; Shenhongtongluo Granules
{"title":"Intervention of Shenhongtongluo Granules on inflammatory response and oxidative stress of atherosclerosisin rabbit","authors":"Yingzi Cui, Ruonan Wang, Lihong Jiang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.06.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.06.007","url":null,"abstract":"Objective \u0000To study the effects of ShenhongTongluo Granules on blood lipids, inflammatory related factors and oxidative stress-related indexes in rabbit atherosclerosis model, and to explore the mechanism of Shenhongtongluo Granules on atherosclerosis. \u0000 \u0000 \u0000Methods \u000070 healthy male New Zealand white rabbits were randomly divided into blank control group, model group, aspirin combined with simvastatin group, aspirin group, simvastatin group, Shenhongtongluo Granule prevention group, and Shenhongtongluo Granule treatment group. The rabbit atherosclerosis model was established by lipidforage diet combined with immune stimulation, and the blank group was fed with commonforage. The Shenhongtongluo Granule prevention group was given Shenhongtongluo Granule at the beginning of modeling, and the other intervention groups were given the corresponding drugs after the successful modeling. Before and after modeling, lipid changes were checked in each group, including total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), superoxide dismutase activity (SOD) and malondialdehyde (MDA) in the serum. The aorta of the model group was separated and oil red O staining was performed to observe the pathological and morphological changes of the aorta. Western blotting and reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of Nox4 and p22phox. Western blotting was used to detect the expression of TLR4 and NF-κB protein in aorta. \u0000 \u0000 \u0000Results \u0000Twelve weeks after modeling, atheromatous plaques were obviously observed on the aorta stained with oil red O, indicating successful atherosclerosis modeling. Compared with the blank control group, TC, TG, and LDL-C in the serum of the atherosclerosis model group were significantly increased (all P<0.05). Compared with the model group, the atherosclerotic plaques in the Shenhongtongluo Granule prevention and the treatment groups were significantly reduced, the levels of TC, TG, LDL-C in serum were decreased, and the levels of SOD were increased, and MDA levels were reduced (all P<0.05). The expressions of TLR4, NF-κB, Nox4, and p22phox in the aorta of Shenhongtongluo Granule prevention group were significantly reduced (all P<0.05). \u0000 \u0000 \u0000Conclusions \u0000Shenhongtongluo Granules can inhibit the formation of atheromatous plaques, have a good effect on regulating blood lipids, and can improve the ability of anti-oxidative stress, and have a good preventive and therapeutic effect. \u0000 \u0000 \u0000Key words: \u0000Atherosclerosis; Oxidative stress; Inflammatory response; Shenhongtongluo Granules","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47463140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.06.002
Hai-Rong Zhao, Jifeng Liu, Chunmei Liu
Objective To study the effect of astragalus polysaccharides combined with hUSCs transplantation on type 2 diabetic rats. Methods Twenty-five SD rats were randomly selected into the normal control group, and the remaining 105 SD rats were used to establish type 2 diabetes model. The 100 rats successfully modeled were randomly divided into the diabetes group, astragalus polysaccharide treatment group, hUSCs treatment group, and astragalus polysaccharide + hUSCs treatment group, with 25 rats in each group. After 2 weeks of treatment, the FBG concentration, insulin and C-peptide concentrations, and body weight changes were measured in each group. The distribution and survival of PKH-26-labeled hUSCs in rat pancreatic tissue were observed by fluorescence microscopy. TUNEL method was used to detect the apoptosis of rat islet cells. Real-time quantitative PCR and Western Blot were used to detect the expression of TGF-β/Smad signaling pathway-related genes in rat pancreatic tissue. Results The FBG concentration of rats in the astragalus polysaccharide treatment group, hUSCs treatment group and astragalus polysaccharide+hUSCs treatment group were significantly decreased, and that in the combination treatment group was significantly lower those in the astragalus polysaccharide group and hUSCs group, and the differences were statistically significant(all P<0.05). Compared with the diabetic group, the insulin concentration, C-peptide concentration and body weight in the astragalus polysaccharide treatment group, hUSCs treatment group and combination treatment group rats were significantly increased, and those in the combination treatment group was significantly higher than those in the astragalus polysaccharide treatment group and in the hUSCs treatment group, the differences were statistically significant (all P<0.05). The results of fluorescence microscopy showed that the number of PKH-26 positive hUSCs in the combined treatment group was 74.64±9.75 in each high power field, which was significantly higher than that in the hUSCs treatment group (43.64±5.83), the difference was statistically significant (P<0.05). Compared with the diabetic group, the apoptotic rates of islet cells in the astragalus polysaccharide treatment group and the hUSCs treatment group were reduced, and the relative expressions levels of mRNA and protein of TGF-β1, Smad3, and Smad7 in the pancreatic tissue were also significantly reduced(all P<0.05). The reduction was more significant in the combination treatment group, and the differences were statistically significant (all P<0.05). Conclusions Astragalus polysaccharide combined with hUSCs transplantation can effectively reduce the FBG concentration, increase the concentration of insulin, C-peptide and body weight, reduce the apoptosis of pancreatic islet tissue, which may be related to the reduction of TGF-β/Smad in pancreatic tissue. Signaling pathways are involved in suppressing the inflammatory response. Ke
{"title":"Effect of astragalus polysaccharide combined with urine stem cell transplantation on type 2 diabetes in rats","authors":"Hai-Rong Zhao, Jifeng Liu, Chunmei Liu","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.06.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.06.002","url":null,"abstract":"Objective \u0000To study the effect of astragalus polysaccharides combined with hUSCs transplantation on type 2 diabetic rats. \u0000 \u0000 \u0000Methods \u0000Twenty-five SD rats were randomly selected into the normal control group, and the remaining 105 SD rats were used to establish type 2 diabetes model. The 100 rats successfully modeled were randomly divided into the diabetes group, astragalus polysaccharide treatment group, hUSCs treatment group, and astragalus polysaccharide + hUSCs treatment group, with 25 rats in each group. After 2 weeks of treatment, the FBG concentration, insulin and C-peptide concentrations, and body weight changes were measured in each group. The distribution and survival of PKH-26-labeled hUSCs in rat pancreatic tissue were observed by fluorescence microscopy. TUNEL method was used to detect the apoptosis of rat islet cells. Real-time quantitative PCR and Western Blot were used to detect the expression of TGF-β/Smad signaling pathway-related genes in rat pancreatic tissue. \u0000 \u0000 \u0000Results \u0000The FBG concentration of rats in the astragalus polysaccharide treatment group, hUSCs treatment group and astragalus polysaccharide+hUSCs treatment group were significantly decreased, and that in the combination treatment group was significantly lower those in the astragalus polysaccharide group and hUSCs group, and the differences were statistically significant(all P<0.05). Compared with the diabetic group, the insulin concentration, C-peptide concentration and body weight in the astragalus polysaccharide treatment group, hUSCs treatment group and combination treatment group rats were significantly increased, and those in the combination treatment group was significantly higher than those in the astragalus polysaccharide treatment group and in the hUSCs treatment group, the differences were statistically significant (all P<0.05). The results of fluorescence microscopy showed that the number of PKH-26 positive hUSCs in the combined treatment group was 74.64±9.75 in each high power field, which was significantly higher than that in the hUSCs treatment group (43.64±5.83), the difference was statistically significant (P<0.05). Compared with the diabetic group, the apoptotic rates of islet cells in the astragalus polysaccharide treatment group and the hUSCs treatment group were reduced, and the relative expressions levels of mRNA and protein of TGF-β1, Smad3, and Smad7 in the pancreatic tissue were also significantly reduced(all P<0.05). The reduction was more significant in the combination treatment group, and the differences were statistically significant (all P<0.05). \u0000 \u0000 \u0000Conclusions \u0000Astragalus polysaccharide combined with hUSCs transplantation can effectively reduce the FBG concentration, increase the concentration of insulin, C-peptide and body weight, reduce the apoptosis of pancreatic islet tissue, which may be related to the reduction of TGF-β/Smad in pancreatic tissue. Signaling pathways are involved in suppressing the inflammatory response. \u0000 \u0000 \u0000Ke","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69894623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.06.001
Lei Wang, Fuchun Zhang
Objective To investigate the effects of ethyl acetate phase of Pleurotus ferulatus triterpenoid component (PFTP-E), oleanolic acid (OA) and paclitaxel (PTX) on growth inhibition and possible regulation mechanisms of colon cancer cells HCT116 and CT26. Methods Ultraviolet and infrared spectroscopy was used to analyze the component of PFTP-E. MTT assay was used to detect the proliferation of HCT116 and CT26 cells by PFTP-E, OA and PTX in vitro. Apoptosis was observed by Hoechst 33258 staining. Flow cytometry was used to detect apoptosis, cell cycle, mitochondrial membrane potential and intracellular ROS. Western Blot were used to detect the expressions of apoptosis-related proteins B cell lymphoma-2 (Bcl-2), BCL2 associated X protein (Bax), poly(adenosine diphosphate-ribose) polymerase (PARP), Caspase-3, Caspase-9, cell cycle related proteinB1 (Cyclin B1), endoplasmic reticulum stress-related proteins glucose-regulated protein 78 (GRP78), protein kinase R-like ER kinase (PERK), eukaryotic initiation factor 2α (eIF2α), C/EBP-homologous protein (CHOP), and autophagy microtubule associated protein light chain 3A/B (LC3A/B). Results PFTP-E is mainly composed of steroids and triterpenoids. PFTP-E, OA and PTX can inhibit the proliferation of HCT116 and CT26 cells in a time- and dose-dependent manner, induce apoptosis, and block the cell cycle at G0/G1 and G2/M phases. PFTP-E can collapse mitochondrial membrane potential of HCT116 and CT26 cells and increase the content of intracellular reactive oxygen species, which is similar to the effect of PTX. PFTP-E and PTX can up-regulate CytC, Bax, GRP78, p-PERK, p-eIF2α and CHOP, down-regulate Bcl-2, significantly increase the content of cleaved PARP, Caspase3 and Caspase9, and induce endoplasmic reticulum stress response. OA can significantly increase the expression of LC3A/B in HCT116 cells and tend to induce autophagy and apoptosis. Conclusions PFTP-E and PTX have a similar effect on apoptosis, and that is related to mitochondrial injury pathway, cycle arrest, and endoplasmic reticulum stress response. The side effects of PFTP-E are significantly lower than PTX, which provides a meaningful reference for the screening and research of PFTP-E as an anticancer drug. Key words: Triterpenoid; Colonic neoplasms; Apoptosis; Pleurotus ferulatus; Endoplasmic reticulum stress
{"title":"In vitro effect of Pleurotus ferulatus total triterpenoids, oleanolic acid and paclitaxel on colon cancer","authors":"Lei Wang, Fuchun Zhang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.06.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.06.001","url":null,"abstract":"Objective \u0000To investigate the effects of ethyl acetate phase of Pleurotus ferulatus triterpenoid component (PFTP-E), oleanolic acid (OA) and paclitaxel (PTX) on growth inhibition and possible regulation mechanisms of colon cancer cells HCT116 and CT26. \u0000 \u0000 \u0000Methods \u0000Ultraviolet and infrared spectroscopy was used to analyze the component of PFTP-E. MTT assay was used to detect the proliferation of HCT116 and CT26 cells by PFTP-E, OA and PTX in vitro. Apoptosis was observed by Hoechst 33258 staining. Flow cytometry was used to detect apoptosis, cell cycle, mitochondrial membrane potential and intracellular ROS. Western Blot were used to detect the expressions of apoptosis-related proteins B cell lymphoma-2 (Bcl-2), BCL2 associated X protein (Bax), poly(adenosine diphosphate-ribose) polymerase (PARP), Caspase-3, Caspase-9, cell cycle related proteinB1 (Cyclin B1), endoplasmic reticulum stress-related proteins glucose-regulated protein 78 (GRP78), protein kinase R-like ER kinase (PERK), eukaryotic initiation factor 2α (eIF2α), C/EBP-homologous protein (CHOP), and autophagy microtubule associated protein light chain 3A/B (LC3A/B). \u0000 \u0000 \u0000Results \u0000PFTP-E is mainly composed of steroids and triterpenoids. PFTP-E, OA and PTX can inhibit the proliferation of HCT116 and CT26 cells in a time- and dose-dependent manner, induce apoptosis, and block the cell cycle at G0/G1 and G2/M phases. PFTP-E can collapse mitochondrial membrane potential of HCT116 and CT26 cells and increase the content of intracellular reactive oxygen species, which is similar to the effect of PTX. PFTP-E and PTX can up-regulate CytC, Bax, GRP78, p-PERK, p-eIF2α and CHOP, down-regulate Bcl-2, significantly increase the content of cleaved PARP, Caspase3 and Caspase9, and induce endoplasmic reticulum stress response. OA can significantly increase the expression of LC3A/B in HCT116 cells and tend to induce autophagy and apoptosis. \u0000 \u0000 \u0000Conclusions \u0000PFTP-E and PTX have a similar effect on apoptosis, and that is related to mitochondrial injury pathway, cycle arrest, and endoplasmic reticulum stress response. The side effects of PFTP-E are significantly lower than PTX, which provides a meaningful reference for the screening and research of PFTP-E as an anticancer drug. \u0000 \u0000 \u0000Key words: \u0000Triterpenoid; Colonic neoplasms; Apoptosis; Pleurotus ferulatus; Endoplasmic reticulum stress","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41421299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.06.010
Xiujuan Han
Objective To evaluate the clinical effect of infrared polarized light irradiation in the treatment of frozen shoulder. Methods A total of 150 patients with frozen shoulder who were treated in the rehabilitation department of Tianjin People's Hospital from October 2015 to December 2018 were randomly divided into a control group (75 cases) and a treatment group (75 cases) using a random number table. Finally, 120 patients who underwent the complete treatment were enrolled including 67 in the treatment group and 53 in the control group. The patients in the treatment group was treated with infrared polarized light irradiation combined with shoulder joint mobilization, and in the control group was treated with shoulder mobilization alone. The whole course is 2 months, and the treatment was 5 times a week. In each treatment, the duration of infrared polarized light irradiation is 10 min and the duration of joint mobilization is 15 to 20 min. Rehabilitation assessment of pain (visual analogue scale, VAS), range of motion (ROM), muscle strength and self-rating anxiety scale (SAS) were performed before the first treatment and after 2 weeks of treatment. Results Before the treatment, there were no statistically significant differences in VAS, ROM, muscle strength and SAS scores between the two groups (all P>0.05). Compared with that before treatment, VAS and SAS scores were significantly decreased, ROM and muscle strength scores were significantly increased in the two groups after 2 weeks treatment (all P 0.05). The improvements of the indicators in the treatment group was greater than that of the control group. Conclusions Infrared polarized light irradiation can significantly improve the treatment effect of frozen shoulder. Key words: Frozen shoulder; Infrared polarized light; Rehabilitation assessment
{"title":"Clinical efficacy of infrared polarized light in the treatment of frozen shoulder","authors":"Xiujuan Han","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.06.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.06.010","url":null,"abstract":"Objective \u0000To evaluate the clinical effect of infrared polarized light irradiation in the treatment of frozen shoulder. \u0000 \u0000 \u0000Methods \u0000A total of 150 patients with frozen shoulder who were treated in the rehabilitation department of Tianjin People's Hospital from October 2015 to December 2018 were randomly divided into a control group (75 cases) and a treatment group (75 cases) using a random number table. Finally, 120 patients who underwent the complete treatment were enrolled including 67 in the treatment group and 53 in the control group. The patients in the treatment group was treated with infrared polarized light irradiation combined with shoulder joint mobilization, and in the control group was treated with shoulder mobilization alone. The whole course is 2 months, and the treatment was 5 times a week. In each treatment, the duration of infrared polarized light irradiation is 10 min and the duration of joint mobilization is 15 to 20 min. Rehabilitation assessment of pain (visual analogue scale, VAS), range of motion (ROM), muscle strength and self-rating anxiety scale (SAS) were performed before the first treatment and after 2 weeks of treatment. \u0000 \u0000 \u0000Results \u0000Before the treatment, there were no statistically significant differences in VAS, ROM, muscle strength and SAS scores between the two groups (all P>0.05). Compared with that before treatment, VAS and SAS scores were significantly decreased, ROM and muscle strength scores were significantly increased in the two groups after 2 weeks treatment (all P 0.05). The improvements of the indicators in the treatment group was greater than that of the control group. \u0000 \u0000 \u0000Conclusions \u0000Infrared polarized light irradiation can significantly improve the treatment effect of frozen shoulder. \u0000 \u0000 \u0000Key words: \u0000Frozen shoulder; Infrared polarized light; Rehabilitation assessment","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45516897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.05.005
Shusheng Hu, Xu Wang, Ruifang Yang, Xiaoyan Wang
Objective To detect the levels of D-dimer (D-D) and fibrinogen (Fbg) in plasma of patients with malignant tumors, and to evaluate the diagnostic value of combined detection of D-D and Fbg. Methods The clinical data of 99 patients with malignant tumors were retrospectively analyzed, including 47 patients in DVT group and 52 patients in control group. DVT diagnosis was based on color Doppler or venography. The ROC curve was used to evaluate the diagnostic value of D-D, Fbg and the combination of D-D and Fbg for the malignant tumor patients with DVT. Results The D-D levels in the DVT group and the control group were 7.17 μg/ml and 4.25 μg/ml, respectively, and the Fbg levels were 4.01 mg/ml and 2.02 mg/ml, respectively, and the differences were statistically significant (all P<0.01). For the area under the ROC curve (AUC), specificity and sensitivity for diagnosing malignant tumors with DVT, D-D were 0.728, 62.50%, and 72.50%, respectively, and Fbg was 0.642, 65.50%, and 58.80%, respectively, and D-D+Fbg was 0.764, 83.30% and 59.00% respectively. Conclusions D-D and Fbg has a significantly high level in malignant tumor patients with DVT. The combined detection of D-D and Fbg has potential application value in the early diagnosis, early intervention and better prognosis for the malignant tumors patients with DVT. Key words: D-dimer; Fibrinogen; Deep venous thrombosis; Tumors
{"title":"Diagnostic value of combined detection of D-dimer and fibrinogen for malignant tumor with deep vein thrombosis","authors":"Shusheng Hu, Xu Wang, Ruifang Yang, Xiaoyan Wang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.05.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.05.005","url":null,"abstract":"Objective \u0000To detect the levels of D-dimer (D-D) and fibrinogen (Fbg) in plasma of patients with malignant tumors, and to evaluate the diagnostic value of combined detection of D-D and Fbg. \u0000 \u0000 \u0000Methods \u0000The clinical data of 99 patients with malignant tumors were retrospectively analyzed, including 47 patients in DVT group and 52 patients in control group. DVT diagnosis was based on color Doppler or venography. The ROC curve was used to evaluate the diagnostic value of D-D, Fbg and the combination of D-D and Fbg for the malignant tumor patients with DVT. \u0000 \u0000 \u0000Results \u0000The D-D levels in the DVT group and the control group were 7.17 μg/ml and 4.25 μg/ml, respectively, and the Fbg levels were 4.01 mg/ml and 2.02 mg/ml, respectively, and the differences were statistically significant (all P<0.01). For the area under the ROC curve (AUC), specificity and sensitivity for diagnosing malignant tumors with DVT, D-D were 0.728, 62.50%, and 72.50%, respectively, and Fbg was 0.642, 65.50%, and 58.80%, respectively, and D-D+Fbg was 0.764, 83.30% and 59.00% respectively. \u0000 \u0000 \u0000Conclusions \u0000D-D and Fbg has a significantly high level in malignant tumor patients with DVT. The combined detection of D-D and Fbg has potential application value in the early diagnosis, early intervention and better prognosis for the malignant tumors patients with DVT. \u0000 \u0000 \u0000Key words: \u0000D-dimer; Fibrinogen; Deep venous thrombosis; Tumors","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41780986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.05.016
Licheng Lu, Xuelian Gu, Jian-Chao Han
Mechanical thrombectomy is a minimally invasive interventional method. Embolization device is too close to the blood vessel wall and venous valve in clinical applications, and it can cause blood vessel damage when it is sucked into the lumen. Embolization device is easy to cause hemolysis. Optimal design can reduce the damage to red blood cells, but it cannot be completely avoided; Shedding emboli can complicate the pulmonary artery. The clinical application studies of mechanical thrombectomy devices in the world was reviewed, including 74 experimental studies and 166 clinical application studies. The current status and causes of vascular injury, hemolysis and pulmonary embolism caused by mechanical thrombectomy were analyzed. This paper aims to make recommendations for the optimization of mechanical thrombectomy devices. Key words: Deep venous thrombosis; Mechanical thrombectomy device; Optimization
{"title":"Research progress in mechanical thrombectomy device for deep venous thrombosis treatment","authors":"Licheng Lu, Xuelian Gu, Jian-Chao Han","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.05.016","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.05.016","url":null,"abstract":"Mechanical thrombectomy is a minimally invasive interventional method. Embolization device is too close to the blood vessel wall and venous valve in clinical applications, and it can cause blood vessel damage when it is sucked into the lumen. Embolization device is easy to cause hemolysis. Optimal design can reduce the damage to red blood cells, but it cannot be completely avoided; Shedding emboli can complicate the pulmonary artery. The clinical application studies of mechanical thrombectomy devices in the world was reviewed, including 74 experimental studies and 166 clinical application studies. The current status and causes of vascular injury, hemolysis and pulmonary embolism caused by mechanical thrombectomy were analyzed. This paper aims to make recommendations for the optimization of mechanical thrombectomy devices. \u0000 \u0000 \u0000Key words: \u0000Deep venous thrombosis; Mechanical thrombectomy device; Optimization","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45004280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.05.015
Yichen Li, Juanjuan Liu, Hong-xiang Guo
Endotoxemia is a pathophysiological manifestation caused by the release of large amounts of endotoxin from bacteria in the blood or in the lesion. It can cause multiple organ failure, irreversible shock, and even death. The mortality rate of endotoxemiaIt is high. Bacterial endotoxin is the main cause of endotoxemia. At present, there is no safe and effective drug to treat endotoxemia in clinic. Research shows that blood purification can effectively reduce endotoxin level in the blood, then achieve the goal of treatment of endotoxemia. In this paper, the pathogenesis of endotoxemia, the development of hemoperfusion therapy technology, the mechanism and research status of endotoxin adsorption by different hemoperfusion resin were discussed, and the performance and safety requirements of hemoperfusion adsorbent materials for endotoxemia treatment were studied, so as to provide theoretical support for the synthesis of new hemoperfusion adsorption materials for the treatment of endotoxemia. Key words: Hemoperfusion; Endotoxemia; Asorption mechanism
{"title":"Mechanism and research status of hemoperfusion in endotoxemia treatment","authors":"Yichen Li, Juanjuan Liu, Hong-xiang Guo","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.05.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.05.015","url":null,"abstract":"Endotoxemia is a pathophysiological manifestation caused by the release of large amounts of endotoxin from bacteria in the blood or in the lesion. It can cause multiple organ failure, irreversible shock, and even death. The mortality rate of endotoxemiaIt is high. Bacterial endotoxin is the main cause of endotoxemia. At present, there is no safe and effective drug to treat endotoxemia in clinic. Research shows that blood purification can effectively reduce endotoxin level in the blood, then achieve the goal of treatment of endotoxemia. In this paper, the pathogenesis of endotoxemia, the development of hemoperfusion therapy technology, the mechanism and research status of endotoxin adsorption by different hemoperfusion resin were discussed, and the performance and safety requirements of hemoperfusion adsorbent materials for endotoxemia treatment were studied, so as to provide theoretical support for the synthesis of new hemoperfusion adsorption materials for the treatment of endotoxemia. \u0000 \u0000 \u0000Key words: \u0000Hemoperfusion; Endotoxemia; Asorption mechanism","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47167602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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