Pub Date : 2019-08-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.04.015
Jing-jing Zhang
High mobility group protein B1 (HMGB1) is encoded by HMGB1 gene and is a highly conserved non-histone chromosomal binding protein widely present in the nucleus. It plays an important role in DNA replication and repair of eukaryotic cells. As a delayed inflammatory mediator, HMGB1 secreted or released extracellularly mediates inflammatory response in sepsis, autoimmune diseases, malignant tumors, liver-related diseases, and so on. In recent years, the studies of HMGB1 gene polymorphism have provided a theoretical basis for further clarifying the pathogenesis of diseases. These studies provided early predictions of disease susceptibility for early intervention and provided the basis for precise prevention and treatment of diseases. In this paper, the correlation between HMGB1 and clinical disease susceptibility and prognosis was reviewed. Key words: HMGB1 protein; HMGB1 gene; Single nucleotide polymorphisms; Sepsis; Malignant tumor
{"title":"Research progress of HMGB1 gene polymorphism related to disease","authors":"Jing-jing Zhang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.04.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.04.015","url":null,"abstract":"High mobility group protein B1 (HMGB1) is encoded by HMGB1 gene and is a highly conserved non-histone chromosomal binding protein widely present in the nucleus. It plays an important role in DNA replication and repair of eukaryotic cells. As a delayed inflammatory mediator, HMGB1 secreted or released extracellularly mediates inflammatory response in sepsis, autoimmune diseases, malignant tumors, liver-related diseases, and so on. In recent years, the studies of HMGB1 gene polymorphism have provided a theoretical basis for further clarifying the pathogenesis of diseases. These studies provided early predictions of disease susceptibility for early intervention and provided the basis for precise prevention and treatment of diseases. In this paper, the correlation between HMGB1 and clinical disease susceptibility and prognosis was reviewed. \u0000 \u0000 \u0000Key words: \u0000HMGB1 protein; HMGB1 gene; Single nucleotide polymorphisms; Sepsis; Malignant tumor","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48551719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.03.008
Ji Ma, Haiyun Wang, Junzhang Xiao, H. Mou
Objective To evaluate the effect of dexmedetomidine on pulmonary function in elderly patients with mild obstructive ventilatory dysfunction under protective ventilation during laparoseopic gallbladder surgery. Methods Sixty patients were included who underwent laparoseopic gallbladder surgery with general anesthesia. For the all subjects, the age ranged from 65 to 75 years old, the body mass index range was 18.5~23.9 kg/m2, and the ASA grade was Ⅱ or Ⅲ. In the subject selection, the gender was not limited, and the included subjects were diagnosed with mild obstructive ventilation dysfunction by preoperative pulmonary function tests. All the subjects were randomly divided into 2 groups (n=30), including dexmedetomidine group (group D) and control group (group C). In the group D, dexmedetomidine was intravenously infused at a dose of 1 μg/kg 10 min before and after the general anesthesia induction, and at a rate of 0.4 μg/(kg·h) after tracheal intubation until the end of operation. Group C was given an equal volume of normal saline. Airway peak pressure (Ppeak), mean airway pressure (Pmean), airway plateau pressure (Pplat), and positive end-expiratory pressure(PEEP) were measured at 5 min after intubation (before pneumoperitoneum) (T1), end of pneumoperjtoneum (T2), and 10 min after the end of pneumoperitoneum (T3). The driving pressure (DP) was calculated. The blood samples of the radial artery were collected for blood gas analysis, and PaO2 and PaCO2 were recorded. The oxygenation index (PaO2/FiO2), respiratory index (RI), dead space ventilation rate (VD/VT), and alveolar-arterial oxygen partial pressure difference (A-aDO2) were calculated. The time of removal of the tracheal tube and the occurrences of complications such as hypercapnia and hypoxemia within 48 hours after extubation were recorded. Results Compared with group C, Ppeak, Pmean, DP, RI, VD/VT and A-aDO2 at T1, T2, T3 were decreased in group D, PaO2/FiO2 was increased, postoperative extubation time was shortened, and the incidence of hypoxemia was reduced within 48 h after operation (P<0.05). Compared with T1, Ppeak, Pmean, DP, RI, VD/VT and A-aDO2 at T2 were increased, and PaO2/FiO2 was decreased(P<0.05). Conclusions Dexmedetomidine can improve the pulmonary function in elderly patients with mild obstructive ventilatory dysfunction under protective ventilation during laparoseopic gallbladder surgery. Key words: Dexmedetomidine; Laparoscopic surgery; Pulmonary function
{"title":"Effect of dexmedetomidine on pulmonary function in elderly patients with mild obstructive ventilatory dysfunction under protective ventilation during laparoseopic gallbladder surgery","authors":"Ji Ma, Haiyun Wang, Junzhang Xiao, H. Mou","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.03.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.03.008","url":null,"abstract":"Objective \u0000To evaluate the effect of dexmedetomidine on pulmonary function in elderly patients with mild obstructive ventilatory dysfunction under protective ventilation during laparoseopic gallbladder surgery. \u0000 \u0000 \u0000Methods \u0000Sixty patients were included who underwent laparoseopic gallbladder surgery with general anesthesia. For the all subjects, the age ranged from 65 to 75 years old, the body mass index range was 18.5~23.9 kg/m2, and the ASA grade was Ⅱ or Ⅲ. In the subject selection, the gender was not limited, and the included subjects were diagnosed with mild obstructive ventilation dysfunction by preoperative pulmonary function tests. All the subjects were randomly divided into 2 groups (n=30), including dexmedetomidine group (group D) and control group (group C). In the group D, dexmedetomidine was intravenously infused at a dose of 1 μg/kg 10 min before and after the general anesthesia induction, and at a rate of 0.4 μg/(kg·h) after tracheal intubation until the end of operation. Group C was given an equal volume of normal saline. Airway peak pressure (Ppeak), mean airway pressure (Pmean), airway plateau pressure (Pplat), and positive end-expiratory pressure(PEEP) were measured at 5 min after intubation (before pneumoperitoneum) (T1), end of pneumoperjtoneum (T2), and 10 min after the end of pneumoperitoneum (T3). The driving pressure (DP) was calculated. The blood samples of the radial artery were collected for blood gas analysis, and PaO2 and PaCO2 were recorded. The oxygenation index (PaO2/FiO2), respiratory index (RI), dead space ventilation rate (VD/VT), and alveolar-arterial oxygen partial pressure difference (A-aDO2) were calculated. The time of removal of the tracheal tube and the occurrences of complications such as hypercapnia and hypoxemia within 48 hours after extubation were recorded. \u0000 \u0000 \u0000Results \u0000Compared with group C, Ppeak, Pmean, DP, RI, VD/VT and A-aDO2 at T1, T2, T3 were decreased in group D, PaO2/FiO2 was increased, postoperative extubation time was shortened, and the incidence of hypoxemia was reduced within 48 h after operation (P<0.05). Compared with T1, Ppeak, Pmean, DP, RI, VD/VT and A-aDO2 at T2 were increased, and PaO2/FiO2 was decreased(P<0.05). \u0000 \u0000 \u0000Conclusions \u0000Dexmedetomidine can improve the pulmonary function in elderly patients with mild obstructive ventilatory dysfunction under protective ventilation during laparoseopic gallbladder surgery. \u0000 \u0000 \u0000Key words: \u0000Dexmedetomidine; Laparoscopic surgery; Pulmonary function","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48042777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.03.016
Xin Guo, Chongzhi Sun
Benign prostatic hyperplasia (BPH) is one of the common urinary system diseases in middle-aged and elderly men, which seriously affects the health and quality of life of patients. At present, the pathogenesis of BPH is still unclear, which critically affects the effective treatment of BPH. In recent years, more and more animal experiments and clinical trials have shown that pollen preparations have a significant inhibitory effect on BPH. In this paper, the pathogenesis of BPH and the related researches on the treatment of BPH by pollen preparations were reviewed, and the effective components of pollen preparations were analyzed, so as to discover the mechanism of BPH inhibition by pollen preparations and finally achieve the purpose of comprehensive utilization. Key words: Pollen; Plant preparations; Benign prostatic hyperplasia; Pathogenesis; Mechanism
{"title":"Research progress in pollen preparation for the treatment of benign prostatic hyperplasia","authors":"Xin Guo, Chongzhi Sun","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.03.016","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.03.016","url":null,"abstract":"Benign prostatic hyperplasia (BPH) is one of the common urinary system diseases in middle-aged and elderly men, which seriously affects the health and quality of life of patients. At present, the pathogenesis of BPH is still unclear, which critically affects the effective treatment of BPH. In recent years, more and more animal experiments and clinical trials have shown that pollen preparations have a significant inhibitory effect on BPH. In this paper, the pathogenesis of BPH and the related researches on the treatment of BPH by pollen preparations were reviewed, and the effective components of pollen preparations were analyzed, so as to discover the mechanism of BPH inhibition by pollen preparations and finally achieve the purpose of comprehensive utilization. \u0000 \u0000 \u0000Key words: \u0000Pollen; Plant preparations; Benign prostatic hyperplasia; Pathogenesis; Mechanism","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41311904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.03.007
Dong Yabin, Lei Wang, Linlin Wang, Qian Li, Lin-yan Chai, Yan Wang
Objective To design a portable electroencephalography(EEG) acquisition system to acquire and analysis steady-state visual potentials (SSVEP). Methods The microprocessor MSP432P401 series MCU was used to control the high-performance integrated analog front end ADS1299 to realize the acquisition, amplification and analog-to-digital (AD) conversion of EEG signals. The digital EEG signal is sent to the host computer for processing by WIFI. Spontaneous EEG signals and steady-state visually evoked EEG signals from 3 healthy subjects were collected to verify system performance. Results The collected signal had a clear α-wave rhythm of closed-eye spontaneous EEG signals. The power spectrum density shows that the steady-state visually induced EEG signal frequency and harmonic frequency peak at the corresponding stimulation frequency, indicating that the system works normally and the performance is good. Conclusions The designed portable EEG acquisition system can accurately collect the spontaneous and induced EEG signals of the human body, which provides technical support for the clinical application of SSVEP technology. Key words: Electroencephalography acquisition; Steady-state visual potentials; ADS1299; WIFI
{"title":"Design of a portable SSVEP signal acquisition system","authors":"Dong Yabin, Lei Wang, Linlin Wang, Qian Li, Lin-yan Chai, Yan Wang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.03.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.03.007","url":null,"abstract":"Objective \u0000To design a portable electroencephalography(EEG) acquisition system to acquire and analysis steady-state visual potentials (SSVEP). \u0000 \u0000 \u0000Methods \u0000The microprocessor MSP432P401 series MCU was used to control the high-performance integrated analog front end ADS1299 to realize the acquisition, amplification and analog-to-digital (AD) conversion of EEG signals. The digital EEG signal is sent to the host computer for processing by WIFI. Spontaneous EEG signals and steady-state visually evoked EEG signals from 3 healthy subjects were collected to verify system performance. \u0000 \u0000 \u0000Results \u0000The collected signal had a clear α-wave rhythm of closed-eye spontaneous EEG signals. The power spectrum density shows that the steady-state visually induced EEG signal frequency and harmonic frequency peak at the corresponding stimulation frequency, indicating that the system works normally and the performance is good. \u0000 \u0000 \u0000Conclusions \u0000The designed portable EEG acquisition system can accurately collect the spontaneous and induced EEG signals of the human body, which provides technical support for the clinical application of SSVEP technology. \u0000 \u0000 \u0000Key words: \u0000Electroencephalography acquisition; Steady-state visual potentials; ADS1299; WIFI","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47910847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.03.002
Dayong Liu, L. Lan, Ruiqi Liu
Objective To observe the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and to investigate the epigenetic regulation of EZH2 inhibitor DZNeP on osteogenic differentiation of hPDLSCs. Methods The hPDLSCs were isolated and cultured, and their proliferation under different concentrations of DZNeP (0, 1, 2, 5 and 10 μmol/L) was detected by MTT. The effects of DZNeP on osteogenic differentiation of hPDLSCs were observed by alkaline phosphatase (ALP) staining and alizarin red staining. The effect of DZNeP on the trimethylation of histone H3K27 in hPDLSCs was detected by immunofluorescence staining. Results Compared with the control group, the proliferation of hPDLSCs after 1, 2, 5 and 10 μmol/L DZNeP treatment for 48 h was significantly decreased, respectively (all P<0.05), and it was concentration-dependent. The result of ALP staining and alizarin red staining showed that DZNeP could promote the expression of early osteogenic markers ALP and the formation of advanced calcified nodules of hPDLSCs. The immunofluorescence staining result showed that the trimethylation fluorescence intensity of histone H3K27 was significantly decreased in the DZNeP group compared with the control group. Conclusions As an EZH2 inhibitor, DZNeP can inhibit the proliferation of hPDLSCs and promote the differentiation of hPDLSCs into osteoblasts in vitro, suggesting that DZNeP can be used as a potential small molecule drug for the treatment of periodontitis. Key words: Epigenetic regulation; EZH2; DZNeP; Human periodontal ligament stem cells; Osteogenic differentiation
{"title":"Effects of EZH2 inhibitor DZNeP on osteogenic differentiation of periodontal ligament stem cells","authors":"Dayong Liu, L. Lan, Ruiqi Liu","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.03.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.03.002","url":null,"abstract":"Objective \u0000To observe the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and to investigate the epigenetic regulation of EZH2 inhibitor DZNeP on osteogenic differentiation of hPDLSCs. \u0000 \u0000 \u0000Methods \u0000The hPDLSCs were isolated and cultured, and their proliferation under different concentrations of DZNeP (0, 1, 2, 5 and 10 μmol/L) was detected by MTT. The effects of DZNeP on osteogenic differentiation of hPDLSCs were observed by alkaline phosphatase (ALP) staining and alizarin red staining. The effect of DZNeP on the trimethylation of histone H3K27 in hPDLSCs was detected by immunofluorescence staining. \u0000 \u0000 \u0000Results \u0000Compared with the control group, the proliferation of hPDLSCs after 1, 2, 5 and 10 μmol/L DZNeP treatment for 48 h was significantly decreased, respectively (all P<0.05), and it was concentration-dependent. The result of ALP staining and alizarin red staining showed that DZNeP could promote the expression of early osteogenic markers ALP and the formation of advanced calcified nodules of hPDLSCs. The immunofluorescence staining result showed that the trimethylation fluorescence intensity of histone H3K27 was significantly decreased in the DZNeP group compared with the control group. \u0000 \u0000 \u0000Conclusions \u0000As an EZH2 inhibitor, DZNeP can inhibit the proliferation of hPDLSCs and promote the differentiation of hPDLSCs into osteoblasts in vitro, suggesting that DZNeP can be used as a potential small molecule drug for the treatment of periodontitis. \u0000 \u0000 \u0000Key words: \u0000Epigenetic regulation; EZH2; DZNeP; Human periodontal ligament stem cells; Osteogenic differentiation","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42088300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.03.005
Yuan-dong Li, Jin Su, Jiayu Li, Aifeng Liu, Jun-tao Zhang
Objective To observe the clinical efficacy of pulsed radiofrequency combined with fixed-point lateral flexion and rotation realignment cervical manipulation in the treatment of cervical spondylotic radiculopathy (CSR). Methods Sixty-three patients with CSR were randomly divided into observation group (n=32) and control group (n=31). The observation group was given pulse radiofrequency combined with fixed-point lateral flexion and rotation realignment cervical manipulation, and the control group was given the pulsed radiofrequency treatment. Visual analog scale (VAS) and Japanese Orthopaedic Association (JOA) scores were obtained before the treatment, 7 days and 3 months after the treatment. The temperature differences of bilateral upper limbs infrared thermal imaging were observed before and 3 months after the treatment in the two groups. The clinical efficacy of the two groups was evaluated by using TCM disease and syndrome diagnosis criteria. Results After 7 days and 3 months of the treatment, the VAS scores of the two groups were lower than those before treatments, the VAS scores of the observation group were lower than the control group, and all the differences were statistically significant (all P 0.05]. Three months after the treatment, the temperature difference of infrared thermography in the upper limbs of the observation group was better than that of the control group (P 0.05). Conclusions The pulsed radiofrequency combined with the fixed-point lateral flexion and rotation realignment cervical manipulation in the treatment of radiculopathic cervical spondylosis was effective in improving pain, function and infrared imaging temperature of upper limbs. Key words: Cervical spondylotic radiculopathy; Pulsed radiofrequency surgery; Fixed-point lateral flexion and rotation realignment cervical manipulation; Treatment Outcome
{"title":"Clinical observation on the treatment of cervical spondylotic radiculopathy by the pulsed radiofrequency combined with the fixed-point lateral flexion and rotation realignment cervical manipulation","authors":"Yuan-dong Li, Jin Su, Jiayu Li, Aifeng Liu, Jun-tao Zhang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.03.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.03.005","url":null,"abstract":"Objective \u0000To observe the clinical efficacy of pulsed radiofrequency combined with fixed-point lateral flexion and rotation realignment cervical manipulation in the treatment of cervical spondylotic radiculopathy (CSR). \u0000 \u0000 \u0000Methods \u0000Sixty-three patients with CSR were randomly divided into observation group (n=32) and control group (n=31). The observation group was given pulse radiofrequency combined with fixed-point lateral flexion and rotation realignment cervical manipulation, and the control group was given the pulsed radiofrequency treatment. Visual analog scale (VAS) and Japanese Orthopaedic Association (JOA) scores were obtained before the treatment, 7 days and 3 months after the treatment. The temperature differences of bilateral upper limbs infrared thermal imaging were observed before and 3 months after the treatment in the two groups. The clinical efficacy of the two groups was evaluated by using TCM disease and syndrome diagnosis criteria. \u0000 \u0000 \u0000Results \u0000After 7 days and 3 months of the treatment, the VAS scores of the two groups were lower than those before treatments, the VAS scores of the observation group were lower than the control group, and all the differences were statistically significant (all P 0.05]. Three months after the treatment, the temperature difference of infrared thermography in the upper limbs of the observation group was better than that of the control group (P 0.05). \u0000 \u0000 \u0000Conclusions \u0000The pulsed radiofrequency combined with the fixed-point lateral flexion and rotation realignment cervical manipulation in the treatment of radiculopathic cervical spondylosis was effective in improving pain, function and infrared imaging temperature of upper limbs. \u0000 \u0000 \u0000Key words: \u0000Cervical spondylotic radiculopathy; Pulsed radiofrequency surgery; Fixed-point lateral flexion and rotation realignment cervical manipulation; Treatment Outcome","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42139914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.03.004
D. Peng, Bo-Ya Zhang, Xi Wang, Lei Wang, Huey-Chuan Cheng, Hua-jiang Dong, Sai Zhang
Objective To investigate the effects of umbilical cord mesenchymal stem cells (UCMSCs) on immune microenvironment and angiogenesis in patients with traumatic brain injury. Methods Cerebrospinal fluid (CSF) samples were divided into 4 groups, including normal group (n=6), traumatic brain injury group (n=6), traumatic brain injury+UCMSCs treatment group (n=6) and craniocerebral trauma + conventional treatment group (n=6). The CSF samples were detected by liquid chromatography-mass spectrometry, and data were collected by data independent acquisition (DIA) technology. The differential proteins were screened by bioinformatics processing, and analyzed by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Results A total of 688 proteins were screened in CSF samples and reliably quantified. There were 38 differential proteins in the CSF of patients with traumatic brain injury after treatment with UCMSCs, including 20 up-regulated proteins and 18 down-regulated proteins. The results of GO analysis and KEGG analysis showed that the differential proteins were mainly immunoregulatory function-related proteins, angiogenesis-related proteins, and various connexins. Conclusions The main possible mechanism of UCMSCs in the treatment of traumatic brain injury is to regulate the stability of the immune microenvironment and to promote the regeneration and reconstruction of damaged brain tissue. Key words: Traumatic brain injury; Mesenchymal stem cell transplantation; Umbilical cord; Cerebral revascularization; Immune microenvironment
{"title":"Effect of umbilical cord mesenchymal stem cells on immune microenvironment and vascular reconstruction in traumatic brain injury","authors":"D. Peng, Bo-Ya Zhang, Xi Wang, Lei Wang, Huey-Chuan Cheng, Hua-jiang Dong, Sai Zhang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.03.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.03.004","url":null,"abstract":"Objective \u0000To investigate the effects of umbilical cord mesenchymal stem cells (UCMSCs) on immune microenvironment and angiogenesis in patients with traumatic brain injury. \u0000 \u0000 \u0000Methods \u0000Cerebrospinal fluid (CSF) samples were divided into 4 groups, including normal group (n=6), traumatic brain injury group (n=6), traumatic brain injury+UCMSCs treatment group (n=6) and craniocerebral trauma + conventional treatment group (n=6). The CSF samples were detected by liquid chromatography-mass spectrometry, and data were collected by data independent acquisition (DIA) technology. The differential proteins were screened by bioinformatics processing, and analyzed by Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. \u0000 \u0000 \u0000Results \u0000A total of 688 proteins were screened in CSF samples and reliably quantified. There were 38 differential proteins in the CSF of patients with traumatic brain injury after treatment with UCMSCs, including 20 up-regulated proteins and 18 down-regulated proteins. The results of GO analysis and KEGG analysis showed that the differential proteins were mainly immunoregulatory function-related proteins, angiogenesis-related proteins, and various connexins. \u0000 \u0000 \u0000Conclusions \u0000The main possible mechanism of UCMSCs in the treatment of traumatic brain injury is to regulate the stability of the immune microenvironment and to promote the regeneration and reconstruction of damaged brain tissue. \u0000 \u0000 \u0000Key words: \u0000Traumatic brain injury; Mesenchymal stem cell transplantation; Umbilical cord; Cerebral revascularization; Immune microenvironment","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46951361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.03.012
Fu Yakang, Yuqiang Zhao, J. Weng, Liu Yaowen
The characterization methods in the field of protein adsorption on biological materials in recent years were reviewed from the aspects of protein adsorption amount, adsorption layer thickness, molecular conformational change after protein adsorption, molecular morphology after protein adsorption, and protein molecule adsorption process simulation. These methods include biochemical analysis, surface plasmon resonance (SPR), dissipative quartz crystal microbalance (QCM-D), ellipsometry (ELM), optical interference reflection (RIFS), attenuated total reflection Fourier transformed infrared spectroscopy (ATR-FTIR), circular dichroism (CD), atomic force microscopy (AFM) and computer molecular simulation techniques. In this paper, the basic principles, the advantages and disadvantages of the above characterization methods and related researches were reviewed. This paper provides a comprehensive and reliable basis for the selection of protein experimental characterization methods in protein adsorption, biomaterial design and other research, and provides new ideas for research in the field of protein. Key words: Protein; Adsorption; Biomaterial; Characterization
{"title":"Research progress in the characterization of protein adsorption on biomaterial surface","authors":"Fu Yakang, Yuqiang Zhao, J. Weng, Liu Yaowen","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.03.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.03.012","url":null,"abstract":"The characterization methods in the field of protein adsorption on biological materials in recent years were reviewed from the aspects of protein adsorption amount, adsorption layer thickness, molecular conformational change after protein adsorption, molecular morphology after protein adsorption, and protein molecule adsorption process simulation. These methods include biochemical analysis, surface plasmon resonance (SPR), dissipative quartz crystal microbalance (QCM-D), ellipsometry (ELM), optical interference reflection (RIFS), attenuated total reflection Fourier transformed infrared spectroscopy (ATR-FTIR), circular dichroism (CD), atomic force microscopy (AFM) and computer molecular simulation techniques. In this paper, the basic principles, the advantages and disadvantages of the above characterization methods and related researches were reviewed. This paper provides a comprehensive and reliable basis for the selection of protein experimental characterization methods in protein adsorption, biomaterial design and other research, and provides new ideas for research in the field of protein. \u0000 \u0000 \u0000Key words: \u0000Protein; Adsorption; Biomaterial; Characterization","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47653467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To evaluate the effect of isokinetic muscle training combined with semiconductor laser on acute knee osteoarthritis (KOA). Methods Ninety-eight KOA patients were randomly divided into 4 groups. All patients were treated with conventional rehabilitation treatment and nursing. Based on that treatment, the group 1 received semiconductor laser irradiation, the group 2 received isokinetic muscle training, and the group 3 received laser irradiation combined with isokinetic muscle training. All patients were assessed with WOMAC osteoarthritis rating scale and affected knee extensor and flexor muscles strength measurement including peak torque, peak work, average power, average work and flex/extend before and 4 weeks after the treatment. Results All of the four treatments can significantly alleviate the condition of acute KOA patients and improve the muscle condition around the ipsilateral knee joint. The isokinetic muscle training improves the knee function of KOA patients better than the laser irradiation treatment. Isokinetic strength training combined with laser irradiation can get the most significant improvement of knee joint pain, stiffness, dysfunction, muscle strength of flexors and extensors in KOA patients. Conclusions The combination of isokinetic muscle training and semiconductor laser irradiation has a significant effect on relieving pain, reducing the stiffness, improving the function of knee of the patients with KOA in the acute phase and improving the muscle strength of the affected lower extremities. That methad is superior to drug therapy, physical therapy, or exercise alone, and is better to solve the problem of relieving symptoms and enhancing function simultaneously. Key words: Knee osteoarthritis; Laser; Isokinetic muscle strength exercise; Rehabilitation
{"title":"Rehabilitation effect of isokinetic muscle training combined with semiconductor laser on acute knee osteoarthritis","authors":"J Zhang, Su-yun Chen, Chun-Fang Wang, Yingying Wang, Ying Zhang","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.03.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.03.006","url":null,"abstract":"Objective \u0000To evaluate the effect of isokinetic muscle training combined with semiconductor laser on acute knee osteoarthritis (KOA). \u0000 \u0000 \u0000Methods \u0000Ninety-eight KOA patients were randomly divided into 4 groups. All patients were treated with conventional rehabilitation treatment and nursing. Based on that treatment, the group 1 received semiconductor laser irradiation, the group 2 received isokinetic muscle training, and the group 3 received laser irradiation combined with isokinetic muscle training. All patients were assessed with WOMAC osteoarthritis rating scale and affected knee extensor and flexor muscles strength measurement including peak torque, peak work, average power, average work and flex/extend before and 4 weeks after the treatment. \u0000 \u0000 \u0000Results \u0000All of the four treatments can significantly alleviate the condition of acute KOA patients and improve the muscle condition around the ipsilateral knee joint. The isokinetic muscle training improves the knee function of KOA patients better than the laser irradiation treatment. Isokinetic strength training combined with laser irradiation can get the most significant improvement of knee joint pain, stiffness, dysfunction, muscle strength of flexors and extensors in KOA patients. \u0000 \u0000 \u0000Conclusions \u0000The combination of isokinetic muscle training and semiconductor laser irradiation has a significant effect on relieving pain, reducing the stiffness, improving the function of knee of the patients with KOA in the acute phase and improving the muscle strength of the affected lower extremities. That methad is superior to drug therapy, physical therapy, or exercise alone, and is better to solve the problem of relieving symptoms and enhancing function simultaneously. \u0000 \u0000 \u0000Key words: \u0000Knee osteoarthritis; Laser; Isokinetic muscle strength exercise; Rehabilitation","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44656017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-28DOI: 10.3760/CMA.J.ISSN.1673-4181.2019.03.011
Zhihao Wang, Boyou Zhang, Jun Ma
Tissue engineering is a comprehensive discipline that combines materials science, life sciences, and engineering to repair, and improve and preserve damaged tissues or organs through cell or tissue reconstruction. In recent years, with the rapid development of tissue engineering technology, tissue engineering trachea has gradually become a new approach to tracheal replacement therapy. However, due to the slender and periodic distribution of the blood vessels supplying the trachea, tracheal grafts cannot obtain sufficient blood supply to maintain its demand, making its vascularization problem one of the major obstacles to the development of tissue engineering trachea. In the construction of tissue engineering trachea, the vascularization strategy of seed cells, tracheal scaffold and growth factors have gradually become the focus of research. In this paper, the current researches on tissue engineering tracheal vascularization were reviewed. Key words: Tissue engineering; Vascularization; Tracheal scaffold; Seed cells; Growth factors
{"title":"Research progress in tissue-engineered tracheal vascularization","authors":"Zhihao Wang, Boyou Zhang, Jun Ma","doi":"10.3760/CMA.J.ISSN.1673-4181.2019.03.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1673-4181.2019.03.011","url":null,"abstract":"Tissue engineering is a comprehensive discipline that combines materials science, life sciences, and engineering to repair, and improve and preserve damaged tissues or organs through cell or tissue reconstruction. In recent years, with the rapid development of tissue engineering technology, tissue engineering trachea has gradually become a new approach to tracheal replacement therapy. However, due to the slender and periodic distribution of the blood vessels supplying the trachea, tracheal grafts cannot obtain sufficient blood supply to maintain its demand, making its vascularization problem one of the major obstacles to the development of tissue engineering trachea. In the construction of tissue engineering trachea, the vascularization strategy of seed cells, tracheal scaffold and growth factors have gradually become the focus of research. In this paper, the current researches on tissue engineering tracheal vascularization were reviewed. \u0000 \u0000 \u0000Key words: \u0000Tissue engineering; Vascularization; Tracheal scaffold; Seed cells; Growth factors","PeriodicalId":61751,"journal":{"name":"国际生物医学工程杂志","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43501278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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