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121. ALS-Sitzung 第 121 次 ALS 会议
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-14 DOI: 10.1007/s00003-024-01502-y
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引用次数: 0
92. Arbeitstagung des ALTS 第 92 期 ALTS 研讨会
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-12 DOI: 10.1007/s00003-024-01500-0
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引用次数: 0
Der virtuelle Supermarkt als innovative Forschungsinfrastruktur: Experiment zur Erhöhung der Salienz für Fleischprodukte mit höherem Haltungsstandard 作为创新研究基础设施的虚拟超市:提高饲养标准较高的肉类产品知名度的实验
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-12 DOI: 10.1007/s00003-024-01488-7
Leonie Bach, Nina Weingarten, Kathrin Barbara Meyer, Ching-Hua Yeh, Irina Dolgopolova, Wen-Xiu Wang, Jutta Roosen, Monika Hartmann

In 2019, Germany introduced a uniform, voluntary labeling system for housing conditions of animal products, in order to enhance transparency in this regard. However, these labels are often overlooked. In this context, the present experimental study conducted a virtual supermarket and investigated whether a stronger emphasis on housing condition would lead to an increase in sales of meat products with higher animal welfare standards. To increase consumer attention, various interventions were tested. Participants were randomly assigned to one of three groups, distinguished by the design of the virtual supermarket as follows: (Group 1) Placement of additional banners above the shelves with housing condition labeling cues; (Group 2) Placement of banners as in Group 1 plus additional positioning of housing condition labels next to the price tag; (Group 3) No specific measures. The developed virtual supermarket provided a realistic research infrastructure for data collection, allowing to test the effectiveness of interventions without the need for direct integration into the operations of a real supermarket. Participants showed a very high level of acceptance for both interventions, which resulted in a slight but non-significant increase in the purchase of meat products with higher housing standards. Most participants found the virtual supermarket highly realistic and user-friendly. Future studies can build upon these findings and further enhance the user-friendliness of the virtual supermarket infrastructure, as well as develop the nature of interventions implemented in virtual supermarkets.

2019 年,德国对动物产品的饲养条件实行了统一的自愿标签制度,以提高这方面的透明度。然而,这些标签往往被忽视。在此背景下,本实验研究开展了一个虚拟超市,调查更加重视饲养条件是否会导致动物福利标准更高的肉类产品销量增加。为了提高消费者的关注度,实验测试了各种干预措施。参与者被随机分配到三组中的一组,虚拟超市的设计区分如下:(第 1 组)在货架上方放置额外的标语,并标明饲养条件;(第 2 组)放置与第 1 组相同的标语,并在价格标签旁额外标明饲养条件;(第 3 组)不采取任何具体措施。所开发的虚拟超市为数据收集提供了一个现实的研究基础设施,可以测试干预措施的有效性,而无需直接融入真实超市的运营中。参与者对这两项干预措施的接受程度都很高,这使得他们购买更高饲养标准肉类产品的数量略有增加,但并不明显。大多数参与者都认为虚拟超市非常逼真,对用户非常友好。今后的研究可以这些研究结果为基础,进一步提高虚拟超市基础设施的用户友好性,并发展虚拟超市中实施的干预措施的性质。
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引用次数: 0
Tierschutz first? Wie wichtig sind unterschiedliche Nachhaltigkeitsaspekte in der Nutztierhaltung: Ein systematischer Vergleich 动物福利第一?畜牧业可持续性的不同方面有多重要:系统比较
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-12 DOI: 10.1007/s00003-024-01480-1
Aurelia Schütz, Ivica Faletar, Winnie Isabel Sonntag, Inken Christoph-Schulz, Achim Spiller

Die landwirtschaftliche Nutztierhaltung steht seit Jahren in der öffentlichen Kritik. Insbesondere die vorherrschenden intensiven Haltungsbedingungen werden von weiten Teilen der Gesellschaft kritisiert und sind auch aus tierwissenschaftlicher Sicht stark verbesserungsbedürftig. Mittlerweile ist aber auch hinreichend bekannt, dass die landwirtschaftliche Nutztierhaltung nicht nur negative Auswirkungen auf das Tierwohl, sondern auch auf Umwelt, Klima oder die menschliche Gesundheit hat. Vor diesem Hintergrund ergeben sich unterschiedliche Zielkonflikte, die eine nachhaltige und zukunftsorientierte Transformation des Sektors erschweren. Gerade die Verbesserung des Tierwohls stellt eine komplexe Aufgabe dar, bei der mögliche Zielkonflikte auch aus gesellschaftlicher Sicht identifiziert und bewertet werden müssen, um dem zunehmenden Akzeptanzverlust entgegenzuwirken. Daher hat die vorliegende Studie in einer Online-Befragung mit Hilfe der Methoden des Best-Worst Scaling (BWS) und Rankings untersucht, wie wichtig den Menschen in Deutschland verschiedene relevante Aspekte in der Nutztierhaltung im Konfliktfall sind. Dabei wurde nicht nur die Bewertung von Aspekten verschiedener Zielkategorien wie Tier-, Umwelt- und Klimaschutz oder menschliche Gesundheit betrachtet, sondern auch einzelne Teilaspekte innerhalb des Tierschutzes bzw. Tierwohls für Mastschweine, Milchkühe, Masthähnchen und Legehennen. Die Ergebnisse zeigen, dass die Befragten unabhängig von der Tierart oder der verwendeten Methode dem Tierschutz und der menschlichen Gesundheit eine höhere Priorität einräumen als anderen Nachhaltigkeitsaspekten und insbesondere Produkteigenschaften. Hinsichtlich des Tierwohls wurden bei allen Tierarten die Aspekte gute Haltungsbedingungen, gute Versorgung mit Futter und Wasser und gute Tiergesundheit am höchsten gewichtet. Die Ergebnisse leisten einen wertvollen Beitrag zur Entwicklung politischer Rahmenbedingungen, die auf eine nachhaltige und gesellschaftlich akzeptierte Transformation der derzeitigen landwirtschaftlichen Nutztierhaltung abzielen.

多年来,农场畜牧业一直是公众批评的对象。尤其是当前的集约化饲养条件受到了社会各界的批评,从动物科学的角度来看也亟待改善。然而,众所周知,畜牧业不仅会对动物福利造成负面影响,还会对环境、气候和人类健康造成负面影响。在此背景下,各种相互冲突的目标使得该行业的可持续和面向未来的转型变得更加困难。特别是,改善动物福利是一项复杂的任务,必须从社会角度识别和评估潜在的目标冲突,以抵消日益丧失的认可度。因此,本研究利用在线调查,借助最佳-最差比例(BWS)和排序方法,分析在发生冲突的情况下,畜牧业的各个相关方面对德国人的重要性。调查不仅考虑了对动物福利、环境和气候保护或人类健康等不同目标类别的评估,还考虑了育肥猪、奶牛、肉鸡和蛋鸡的动物福利中的各个子方面。结果表明,无论动物种类或使用的方法如何,受访者都会优先考虑动物福利和人类健康,而不是其他可持续发展方面,尤其是产品特性。在动物福利方面,良好的饲养条件、充足的饲料和水供应以及良好的动物健康在所有动物种类中都是最优先考虑的方面。研究结果为制定一个政治框架做出了宝贵贡献,该框架旨在对当前的畜牧业进行可持续的、为社会所接受的改革。
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引用次数: 0
Medienberichterstattung über Nutztierhaltung: Wahrnehmungen und Bewertung in der deutschen Bevölkerung 媒体对畜牧业的报道:德国民众的看法和评价
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-12 DOI: 10.1007/s00003-024-01481-0
Bea Bardusch, Anna Schulze Walgern, Inken Christoph-Schulz, Marcus Mergenthaler

Die Berichterstattung der Medien über die landwirtschaftliche Nutztierhaltung erfüllt eine doppelte Funktion: Einerseits fungiert sie als wichtige Verbindung zwischen der landwirtschaftlichen Nutztierhaltung und der Öffentlichkeit. Andererseits wird die Medienberichterstattung als potenzielle Ursache für wachsende Unterschiede und als Einflussfaktor für kritische Haltungen in der Bevölkerung angesehen. Im Jahr 2022 wurde daher eine repräsentative Umfrage unter Bürgerinnen und Bürgern in Deutschland durchgeführt, um zu untersuchen, wie verschiedene demografische Gruppen die Medienberichterstattung über die landwirtschaftliche Nutztierhaltung wahrnehmen und bewerten. Die Ergebnisse zeigten signifikante Zusammenhänge zwischen soziodemografischen Merkmalen und der Bewertung der Medienberichte. Insbesondere Frauen und jüngere Personen tendieren dazu, die Medienberichterstattung und die landwirtschaftliche Nutztierhaltung kritischer zu bewerten. Im Gegensatz dazu nehmen Männer und ältere Personen die landwirtschaftliche Nutztierhaltung tendenziell positiver wahr, als sie in den Medien dargestellt wird.

媒体对畜牧业的报道具有双重功能:一方面,它是畜牧业与公众之间的重要纽带。另一方面,媒体报道也被认为是导致分歧不断扩大的潜在原因,是影响民众批判态度的一个因素。因此,我们在 2022 年对德国公民进行了一次代表性调查,研究不同人口群体如何看待和评价媒体对畜牧业的报道。结果显示,社会人口特征与媒体报道的评价之间存在明显的相关性。女性和年轻人尤其倾向于对媒体报道和农场畜牧业持批评态度。相比之下,男性和老年人对畜牧业的看法往往比媒体报道的更为积极。
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引用次数: 0
Ausgewählte Sichtweisen von Landwirtinnen und Landwirten auf die Nutztierhaltung in Deutschland 德国农民对畜牧业的部分看法
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-12 DOI: 10.1007/s00003-024-01491-y
Laura Schmitz, Miriam Kemnade, Marcus Mergenthaler

Several studies have shown that farmers' perceptions of livestock production are influenced by a variety of factors. This paper examines farmers' perceptions of livestock production and animal welfare based on survey data. It was found that farmers see themselves as having the greatest responsibility for ensuring animal welfare, for which they would like to see a stable political framework and support from society. Furthermore, organic livestock production (except for dairy and suckler cows) got better ratings than conventional livestock production. The aspects perceived as the most important for animal welfare varied according to species and type of farming. This survey design approach indents to serve as a long-term tool for the Federal Ministry of Agriculture, in order to monitor the situation in livestock production.

摘要 根据多项研究,农场对畜牧业的看法受到不同因素的影响。本文利用调查数据分析了畜牧业主对农业畜牧业和动物福利的看法。调查发现,畜牧业主认为自己对确保动物福利负有最大责任,为此他们希望看到一个稳定的政治框架和社会的支持。此外,有机畜牧业(奶牛和乳牛畜牧业除外)比传统条件下的畜牧业更好。认为对动物福利最重要的方面因动物类型和管理而异。所采用的调查设计可作为记录畜牧业状况的长期监测工具。
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引用次数: 0
Optimizing exposure data collection for plant protection products: identifying ideal collectors with the fluorescent dye pyranine 优化植物保护产品的暴露数据收集:利用荧光染料吡喃确定理想的收集器
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-03-05 DOI: 10.1007/s00003-024-01498-5
Annika Ehmke, Jens Karl Wegener, Andreas Melfsen, Eberhard Hartung

When approving plant protection products, exposure data are required for risk analysis. Exposure data can be collected for various exposure pathways, such as dermal, inhalation or ground sediment. For measuring exposures, pyranine, a fluorescent dye, and a collector can be used. However, the choice of collector material depends on the specific exposure pathway. This study aims to determine the most suitable collector, in combination with the tracer pyranine, for recording exposure through different pathways in practical trials.

Seven different collectors (Tyvek®, labels, plastic patches, paper patches, nylon filters, fibreglass filters, petri dishes) were subjected to laboratory and field tests to assess various quality parameters. Blank values, recovery rates, storability, and fluorescence degradation under UV-radiation were measured. Based on the results, a matrix was created summarizing which collector might be best suited to capture each exposure pathway. Almost all collectors demonstrated high recovery rates (Tyvek® 100%; labels 100%; plastic patches 100%; paper patches 100%; nylon filters 95%; fiberglass filters 60.9%) as well as good storability. Furthermore, all plastic-based collectors (labels, Tyvek®, plastic patches, petri dishes) showed a very good recovery rate above 95% when exposed to UV-radiation. However, nylon filters were not suitable for utilization under field conditions due to the rapid degradation of fluorescence under UV-radiation (recovery rate: 20–56%). Nevertheless, nylon filters showed stable recoveries under protected conditions and can be used to assess inhalation exposures under these conditions. Fibreglass filters, with recovery rates of 84–86%, were well-suited for testing inhalative exposures outdoors when a correction factor was applied. Tyvek® was the most suitable material for detecting total dermal exposure under field conditions. This is due to its quality characteristics and availability in a variety of sizes. Finally, petri dishes were ideal for collecting ground sediments.

在批准植物保护产品时,需要接触数据来进行风险分析。可以收集各种暴露途径的暴露数据,如皮肤、吸入或地面沉积物。在测量暴露时,可以使用吡喃染料、荧光染料和收集器。不过,收集器材料的选择取决于具体的暴露途径。七种不同的收集器(特卫强®、标签、塑料贴片、纸贴片、尼龙过滤器、玻璃纤维过滤器、培养皿)经过了实验室和现场测试,以评估各种质量参数。对空白值、回收率、储存性和紫外线照射下的荧光降解进行了测量。根据测试结果,创建了一个矩阵,总结出哪种收集器最适合捕捉每种暴露途径。几乎所有收集器都表现出较高的回收率(特卫强® 100%;标签 100%;塑料贴片 100%;纸贴片 100%;尼龙过滤器 95%;玻璃纤维过滤器 60.9%)和良好的储存性。此外,所有基于塑料的收集器(标签、特卫强®、塑料贴片、培养皿)在紫外线照射下的回收率都超过了 95%。不过,尼龙过滤器在紫外线照射下荧光会迅速衰减(回收率:20-56%),因此不适合在野外条件下使用。不过,尼龙过滤器在受保护的条件下显示出稳定的恢复率,可用于评估在这些条件下的吸入辐照。玻璃纤维过滤器的回收率为 84-86%,在应用校正因子时非常适合用于室外吸入辐照测试。特卫强® 是最适合在野外条件下检测皮肤总暴露量的材料。这归功于其质量特性和各种尺寸的可用性。最后,培养皿是收集地面沉积物的理想材料。
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引用次数: 0
Development and validation of a multiplex real-time PCR method for screening genetically modified plants 开发和验证用于筛选转基因植物的多重实时 PCR 方法
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-24 DOI: 10.1007/s00003-024-01499-4
Christopher Weidner, René Köppel, Regina Freyer, Petra Richl, Kathrin Lieske, Joachim Mankertz, Hans-Ulrich Waiblinger

The diversity of genetically modified plants used for food and feed is increasing worldwide. For the detection and control of these products, efficient and reliable analytical tools are a prerequisite. This can be done by screening for specific DNA-elements and constructs characteristic of transgenic plants. In the past, numerous methods have already been published. However, several genetically modified plants are not covered by common screening methods. Here, a new qualitative triplex real-time polymerase chain reaction (PCR) method is presented, detecting two transgene flanking sequences and the transition between the Cassava Vein Mosaic Virus Promotor (P-CsVMV) and the phosphinothricin-N-acetyltransferase (pat) gene. These sequences are present in several transgenic plants and therefore, the described triplex method can be used as a screening tool to guide further analysis and increase the efficiency of the analysis strategy for GMO detection. The method is characterized by high specificity, sensitivity and robustness and is provided as a ring-trial validated method in the Official Collection of Methods according to the German Food and Feed Act.

全球范围内用于食品和饲料的转基因植物日益多样化。要检测和控制这些产品,高效可靠的分析工具是先决条件。这可以通过筛选转基因植物特有的 DNA 元素和构建体来实现。过去,已经有许多方法发表。然而,一些转基因植物并不在常用筛选方法的范围内。本文介绍了一种新的定性三重实时聚合酶链反应(PCR)方法,该方法可检测两个转基因侧翼序列以及木薯花叶病毒启动子(P-CsVMV)和膦丝菌素-N-乙酰转移酶(pat)基因之间的过渡。这些序列存在于几种转基因植物中,因此,所述三重法可用作筛选工具,指导进一步的分析,并提高转基因生物检测分析策略的效率。该方法具有特异性高、灵敏度高和稳健性强的特点,已作为环试验证方法列入《德国食品和饲料法官方方法集》。
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引用次数: 0
Antimicrobial residues in meat from chickens in Northeast Vietnam: analytical validation and pilot study for sampling optimisation 越南东北部鸡肉中的抗菌剂残留:分析验证和优化采样的试点研究
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-16 DOI: 10.1007/s00003-024-01478-9
Sophie Hedges, Ludovic Pelligand, Liwei Chen, Kelyn Seow, Thuy Thi Hoang, Huong Quynh Luu, Son Thi Thanh Dang, Ngoc Thi Pham, Hoa Thi Thanh Pham, Yeong Cheng Cheah, Yulan Wang, Dominique Hurtaud-Pessel, Anne Conan, Guillaume Fournié, Damer Blake, Fiona Tomley, Patricia L. Conway

Antimicrobials used in chicken farming for therapeutic and/or prophylactic purposes may result in unacceptable levels of edible residues, if withdrawal periods are not respected. To evaluate the risk in Vietnam, we validated an analytical method to detect antimicrobial residues from chicken meat samples and carried out a pilot cross-sectional study to identify optimal sampling strategies. A total of 45 raw meat samples were collected from 4 markets, 1 slaughterhouse and 4 farms (5 per site) in Northern Vietnam, between March and April 2021. Farmers were asked about antimicrobials used during sampled production cycles (5 chickens sampled per batch). Samples were analysed using liquid chromatography-tandem mass spectrometry for the presence of 68 antimicrobials at a pre-defined validation concentration. 7 compounds were identified from 4 classes (tetracyclines, sulphonamides, macrolides, and fluoroquinolones). In markets, where the source of sampled chickens was unknown, a diverse pool of residual antimicrobials was detected in 20% (4/20) of the meat samples. No residues were detected in samples from the slaughterhouse. No residues were detected in chickens from the one farm that reported using antimicrobials, whereas sulfadimethoxine, doxycycline and tilmicosin residues were identified from the other 3 farms reporting no antimicrobial use. The probability of detecting antimicrobial residues present in a flock based on sampling a single chicken was estimated at 0.93 (highest density interval 0.735–0.997). The preliminary results suggest a disparity between farmers’ reports on antimicrobial drug use and actual usage, and that the analysis of a single sample per farm has a high probability of detecting antimicrobial residues, if present.

如果不遵守停药期规定,养鸡业中用于治疗和/或预防目的的抗菌剂可能会导致不可接受的食用残留水平。为了评估越南的风险,我们验证了从鸡肉样本中检测抗菌剂残留的分析方法,并开展了一项试点横断面研究,以确定最佳采样策略。2021 年 3 月至 4 月期间,我们从越南北部的 4 个市场、1 个屠宰场和 4 个农场(每个地点 5 个)共采集了 45 份生肉样本。养殖户被问及在采样生产周期内使用抗菌素的情况(每批采样 5 只鸡)。采用液相色谱-串联质谱法对样本进行分析,以确定68种抗菌剂的存在,并预先设定了验证浓度。从 4 个类别(四环素类、磺胺类、大环内酯类和氟喹诺酮类)中鉴定出 7 种化合物。在鸡肉样本来源不明的市场,20%(4/20)的肉类样本中检测到多种残留抗菌素。来自屠宰场的样本未检测到残留抗菌素。在一个报告使用抗菌素的农场的鸡肉中未检测到残留抗菌素,而在另外三个报告未使用抗菌素的农场的鸡肉中检测到磺胺二甲氧嘧啶、强力霉素和替米考星残留。根据对单只鸡的采样,在鸡群中检测到抗菌素残留的概率估计为 0.93(最高密度区间为 0.735-0.997)。初步结果表明,养殖户对抗菌药物使用情况的报告与实际使用情况之间存在差异,如果存在抗菌药物残留,对每个养殖场的单个样本进行分析,检测出抗菌药物残留的概率很高。
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引用次数: 0
Staphylococcus aureus lytic bacteriophage: isolation and application evaluation 金黄色葡萄球菌溶菌噬菌体:分离与应用评估
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-02-15 DOI: 10.1007/s00003-024-01479-8
Fatmawati Lambuk, Nurzafirah Mazlan, Dalene Lesen, Elexson Nillian, Tze Young Thung

Staphylococcus aureus is a significant pathogen associated with various illnesses, including food poisoning. The development of effective treatments is challenging, necessitating the exploration of novel antimicrobial options. Bacteriophages (phages) have emerged as promising candidates in this regard. In this study, a virulent phage called mSA4 was isolated and characterized. Furthermore, its efficacy in combating S. aureus biofilms and growth in various food products was evaluated. Phage mSA4 demonstrated a broad host range, targeting both S. aureus and methicillin-resistant S. aureus strains. Belonging to the Myoviridae family, it exhibited rapid adsorption (over 50% in 3 min), a short latent period (20 min), and a burst size of 97 phage particles per infected bacterial cell. Furthermore, phage mSA4 displayed stability across a wide range of pH values and temperatures, and effectively degraded established biofilms. Its performance was evaluated in chocolate milk, beef meat, and iceberg lettuce, resulting in significant reductions in bacterial counts (2.1 log CFU/mL, 2.8 log CFU/cm2, and 3.2 log CFU/cm2, respectively). These findings underscore the potential of phage mSA4 as a natural biocontrol agent against S. aureus.

Graphical abstract

金黄色葡萄球菌是与食物中毒等各种疾病相关的重要病原体。开发有效的治疗方法具有挑战性,因此有必要探索新的抗菌方案。在这方面,噬菌体(噬菌体)已成为有希望的候选药物。本研究分离并鉴定了一种名为 mSA4 的毒性噬菌体。此外,还评估了它对金黄色葡萄球菌生物膜和在各种食品中生长的抗菌效果。噬菌体 mSA4 的宿主范围很广,既能针对金黄色葡萄球菌,也能针对耐甲氧西林金黄色葡萄球菌。该噬菌体属于肌病毒科,吸附速度快(3 分钟内吸附超过 50%),潜伏期短(20 分钟),每感染一个细菌细胞可产生 97 个噬菌体颗粒。此外,噬菌体 mSA4 在广泛的 pH 值和温度范围内都表现出稳定性,并能有效降解已形成的生物膜。在巧克力牛奶、牛肉和冰山莴苣中对其性能进行了评估,结果显示细菌数量显著减少(分别为 2.1 log CFU/mL、2.8 log CFU/cm2 和 3.2 log CFU/cm2)。这些发现凸显了噬菌体 mSA4 作为天然生物控制剂对付金黄色葡萄球菌的潜力。
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引用次数: 0
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