Pub Date : 2024-03-18DOI: 10.1007/s00003-024-01489-6
Nadine R. Gier-Reinartz, Regina Harms, Peter Kenning
As significant economic stakeholders, consumers also influence the economic development of agricultural livestock farming. Through their purchasing behavior, they collectively play a crucial role in financing potential transformation costs. One aspect influencing consumer behavior is their immediate and concrete decision context at the point of sale, which includes pricing and product availability. Many studies tend to neglect this specific context. This neglect may contribute to the often discussed discrepancy between expressed attitude and observable buying behavior. This reinforces the impression that consumer often demand higher standards for agricultural livestock farming, but do not consistently purchase corresponding products. To bridge this gap between attitude and behavior, it appears sensible to collaborate with all relevant stakeholders to develop and test communication and information strategies at the point of sale. This opportunity is provided by living labs (i.e. “RealLabor”). Against this background, this paper outlines the “RealLabor” as a method for transformation research and the systematic development and prototypical testing of innovative approaches in the market for sustainable consumption.
{"title":"Das RealLabor als Methode der Transformation zur systematischen Entwicklung und prototypischen Erprobung innovativer Marktleistungen für nachhaltigeren Konsum − ein Zwischenbericht","authors":"Nadine R. Gier-Reinartz, Regina Harms, Peter Kenning","doi":"10.1007/s00003-024-01489-6","DOIUrl":"10.1007/s00003-024-01489-6","url":null,"abstract":"<div><p>As significant economic stakeholders, consumers also influence the economic development of agricultural livestock farming. Through their purchasing behavior, they collectively play a crucial role in financing potential transformation costs. One aspect influencing consumer behavior is their immediate and concrete decision context at the point of sale, which includes pricing and product availability. Many studies tend to neglect this specific context. This neglect may contribute to the often discussed discrepancy between expressed attitude and observable buying behavior. This reinforces the impression that consumer often demand higher standards for agricultural livestock farming, but do not consistently purchase corresponding products. To bridge this gap between attitude and behavior, it appears sensible to collaborate with all relevant stakeholders to develop and test communication and information strategies at the point of sale. This opportunity is provided by living labs (i.e. “RealLabor”). Against this background, this paper outlines the “RealLabor” as a method for transformation research and the systematic development and prototypical testing of innovative approaches in the market for sustainable consumption.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 1 supplement","pages":"125 - 136"},"PeriodicalIF":1.4,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01489-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140167496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-12DOI: 10.1007/s00003-024-01500-0
{"title":"92. Arbeitstagung des ALTS","authors":"","doi":"10.1007/s00003-024-01500-0","DOIUrl":"10.1007/s00003-024-01500-0","url":null,"abstract":"","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 3","pages":"365 - 368"},"PeriodicalIF":1.4,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140248856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In 2019, Germany introduced a uniform, voluntary labeling system for housing conditions of animal products, in order to enhance transparency in this regard. However, these labels are often overlooked. In this context, the present experimental study conducted a virtual supermarket and investigated whether a stronger emphasis on housing condition would lead to an increase in sales of meat products with higher animal welfare standards. To increase consumer attention, various interventions were tested. Participants were randomly assigned to one of three groups, distinguished by the design of the virtual supermarket as follows: (Group 1) Placement of additional banners above the shelves with housing condition labeling cues; (Group 2) Placement of banners as in Group 1 plus additional positioning of housing condition labels next to the price tag; (Group 3) No specific measures. The developed virtual supermarket provided a realistic research infrastructure for data collection, allowing to test the effectiveness of interventions without the need for direct integration into the operations of a real supermarket. Participants showed a very high level of acceptance for both interventions, which resulted in a slight but non-significant increase in the purchase of meat products with higher housing standards. Most participants found the virtual supermarket highly realistic and user-friendly. Future studies can build upon these findings and further enhance the user-friendliness of the virtual supermarket infrastructure, as well as develop the nature of interventions implemented in virtual supermarkets.
{"title":"Der virtuelle Supermarkt als innovative Forschungsinfrastruktur: Experiment zur Erhöhung der Salienz für Fleischprodukte mit höherem Haltungsstandard","authors":"Leonie Bach, Nina Weingarten, Kathrin Barbara Meyer, Ching-Hua Yeh, Irina Dolgopolova, Wen-Xiu Wang, Jutta Roosen, Monika Hartmann","doi":"10.1007/s00003-024-01488-7","DOIUrl":"10.1007/s00003-024-01488-7","url":null,"abstract":"<div><p>In 2019, Germany introduced a uniform, voluntary labeling system for housing conditions of animal products, in order to enhance transparency in this regard. However, these labels are often overlooked. In this context, the present experimental study conducted a virtual supermarket and investigated whether a stronger emphasis on housing condition would lead to an increase in sales of meat products with higher animal welfare standards. To increase consumer attention, various interventions were tested. Participants were randomly assigned to one of three groups, distinguished by the design of the virtual supermarket as follows: (Group 1) Placement of additional banners above the shelves with housing condition labeling cues; (Group 2) Placement of banners as in Group 1 plus additional positioning of housing condition labels next to the price tag; (Group 3) No specific measures. The developed virtual supermarket provided a realistic research infrastructure for data collection, allowing to test the effectiveness of interventions without the need for direct integration into the operations of a real supermarket. Participants showed a very high level of acceptance for both interventions, which resulted in a slight but non-significant increase in the purchase of meat products with higher housing standards. Most participants found the virtual supermarket highly realistic and user-friendly. Future studies can build upon these findings and further enhance the user-friendliness of the virtual supermarket infrastructure, as well as develop the nature of interventions implemented in virtual supermarkets.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 1 supplement","pages":"111 - 123"},"PeriodicalIF":1.4,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01488-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140116084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Die landwirtschaftliche Nutztierhaltung steht seit Jahren in der öffentlichen Kritik. Insbesondere die vorherrschenden intensiven Haltungsbedingungen werden von weiten Teilen der Gesellschaft kritisiert und sind auch aus tierwissenschaftlicher Sicht stark verbesserungsbedürftig. Mittlerweile ist aber auch hinreichend bekannt, dass die landwirtschaftliche Nutztierhaltung nicht nur negative Auswirkungen auf das Tierwohl, sondern auch auf Umwelt, Klima oder die menschliche Gesundheit hat. Vor diesem Hintergrund ergeben sich unterschiedliche Zielkonflikte, die eine nachhaltige und zukunftsorientierte Transformation des Sektors erschweren. Gerade die Verbesserung des Tierwohls stellt eine komplexe Aufgabe dar, bei der mögliche Zielkonflikte auch aus gesellschaftlicher Sicht identifiziert und bewertet werden müssen, um dem zunehmenden Akzeptanzverlust entgegenzuwirken. Daher hat die vorliegende Studie in einer Online-Befragung mit Hilfe der Methoden des Best-Worst Scaling (BWS) und Rankings untersucht, wie wichtig den Menschen in Deutschland verschiedene relevante Aspekte in der Nutztierhaltung im Konfliktfall sind. Dabei wurde nicht nur die Bewertung von Aspekten verschiedener Zielkategorien wie Tier-, Umwelt- und Klimaschutz oder menschliche Gesundheit betrachtet, sondern auch einzelne Teilaspekte innerhalb des Tierschutzes bzw. Tierwohls für Mastschweine, Milchkühe, Masthähnchen und Legehennen. Die Ergebnisse zeigen, dass die Befragten unabhängig von der Tierart oder der verwendeten Methode dem Tierschutz und der menschlichen Gesundheit eine höhere Priorität einräumen als anderen Nachhaltigkeitsaspekten und insbesondere Produkteigenschaften. Hinsichtlich des Tierwohls wurden bei allen Tierarten die Aspekte gute Haltungsbedingungen, gute Versorgung mit Futter und Wasser und gute Tiergesundheit am höchsten gewichtet. Die Ergebnisse leisten einen wertvollen Beitrag zur Entwicklung politischer Rahmenbedingungen, die auf eine nachhaltige und gesellschaftlich akzeptierte Transformation der derzeitigen landwirtschaftlichen Nutztierhaltung abzielen.
{"title":"Tierschutz first? Wie wichtig sind unterschiedliche Nachhaltigkeitsaspekte in der Nutztierhaltung: Ein systematischer Vergleich","authors":"Aurelia Schütz, Ivica Faletar, Winnie Isabel Sonntag, Inken Christoph-Schulz, Achim Spiller","doi":"10.1007/s00003-024-01480-1","DOIUrl":"10.1007/s00003-024-01480-1","url":null,"abstract":"<p>Die landwirtschaftliche Nutztierhaltung steht seit Jahren in der öffentlichen Kritik. Insbesondere die vorherrschenden intensiven Haltungsbedingungen werden von weiten Teilen der Gesellschaft kritisiert und sind auch aus tierwissenschaftlicher Sicht stark verbesserungsbedürftig. Mittlerweile ist aber auch hinreichend bekannt, dass die landwirtschaftliche Nutztierhaltung nicht nur negative Auswirkungen auf das Tierwohl, sondern auch auf Umwelt, Klima oder die menschliche Gesundheit hat. Vor diesem Hintergrund ergeben sich unterschiedliche Zielkonflikte, die eine nachhaltige und zukunftsorientierte Transformation des Sektors erschweren. Gerade die Verbesserung des Tierwohls stellt eine komplexe Aufgabe dar, bei der mögliche Zielkonflikte auch aus gesellschaftlicher Sicht identifiziert und bewertet werden müssen, um dem zunehmenden Akzeptanzverlust entgegenzuwirken. Daher hat die vorliegende Studie in einer Online-Befragung mit Hilfe der Methoden des Best-Worst Scaling (BWS) und Rankings untersucht, wie wichtig den Menschen in Deutschland verschiedene relevante Aspekte in der Nutztierhaltung im Konfliktfall sind. Dabei wurde nicht nur die Bewertung von Aspekten verschiedener Zielkategorien wie Tier-, Umwelt- und Klimaschutz oder menschliche Gesundheit betrachtet, sondern auch einzelne Teilaspekte innerhalb des Tierschutzes bzw. Tierwohls für Mastschweine, Milchkühe, Masthähnchen und Legehennen. Die Ergebnisse zeigen, dass die Befragten unabhängig von der Tierart oder der verwendeten Methode dem Tierschutz und der menschlichen Gesundheit eine höhere Priorität einräumen als anderen Nachhaltigkeitsaspekten und insbesondere Produkteigenschaften. Hinsichtlich des Tierwohls wurden bei allen Tierarten die Aspekte gute Haltungsbedingungen, gute Versorgung mit Futter und Wasser und gute Tiergesundheit am höchsten gewichtet. Die Ergebnisse leisten einen wertvollen Beitrag zur Entwicklung politischer Rahmenbedingungen, die auf eine nachhaltige und gesellschaftlich akzeptierte Transformation der derzeitigen landwirtschaftlichen Nutztierhaltung abzielen.</p>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 1 supplement","pages":"21 - 27"},"PeriodicalIF":1.4,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01480-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140116080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-12DOI: 10.1007/s00003-024-01481-0
Bea Bardusch, Anna Schulze Walgern, Inken Christoph-Schulz, Marcus Mergenthaler
Die Berichterstattung der Medien über die landwirtschaftliche Nutztierhaltung erfüllt eine doppelte Funktion: Einerseits fungiert sie als wichtige Verbindung zwischen der landwirtschaftlichen Nutztierhaltung und der Öffentlichkeit. Andererseits wird die Medienberichterstattung als potenzielle Ursache für wachsende Unterschiede und als Einflussfaktor für kritische Haltungen in der Bevölkerung angesehen. Im Jahr 2022 wurde daher eine repräsentative Umfrage unter Bürgerinnen und Bürgern in Deutschland durchgeführt, um zu untersuchen, wie verschiedene demografische Gruppen die Medienberichterstattung über die landwirtschaftliche Nutztierhaltung wahrnehmen und bewerten. Die Ergebnisse zeigten signifikante Zusammenhänge zwischen soziodemografischen Merkmalen und der Bewertung der Medienberichte. Insbesondere Frauen und jüngere Personen tendieren dazu, die Medienberichterstattung und die landwirtschaftliche Nutztierhaltung kritischer zu bewerten. Im Gegensatz dazu nehmen Männer und ältere Personen die landwirtschaftliche Nutztierhaltung tendenziell positiver wahr, als sie in den Medien dargestellt wird.
{"title":"Medienberichterstattung über Nutztierhaltung: Wahrnehmungen und Bewertung in der deutschen Bevölkerung","authors":"Bea Bardusch, Anna Schulze Walgern, Inken Christoph-Schulz, Marcus Mergenthaler","doi":"10.1007/s00003-024-01481-0","DOIUrl":"10.1007/s00003-024-01481-0","url":null,"abstract":"<p>Die Berichterstattung der Medien über die landwirtschaftliche Nutztierhaltung erfüllt eine doppelte Funktion: Einerseits fungiert sie als wichtige Verbindung zwischen der landwirtschaftlichen Nutztierhaltung und der Öffentlichkeit. Andererseits wird die Medienberichterstattung als potenzielle Ursache für wachsende Unterschiede und als Einflussfaktor für kritische Haltungen in der Bevölkerung angesehen. Im Jahr 2022 wurde daher eine repräsentative Umfrage unter Bürgerinnen und Bürgern in Deutschland durchgeführt, um zu untersuchen, wie verschiedene demografische Gruppen die Medienberichterstattung über die landwirtschaftliche Nutztierhaltung wahrnehmen und bewerten. Die Ergebnisse zeigten signifikante Zusammenhänge zwischen soziodemografischen Merkmalen und der Bewertung der Medienberichte. Insbesondere Frauen und jüngere Personen tendieren dazu, die Medienberichterstattung und die landwirtschaftliche Nutztierhaltung kritischer zu bewerten. Im Gegensatz dazu nehmen Männer und ältere Personen die landwirtschaftliche Nutztierhaltung tendenziell positiver wahr, als sie in den Medien dargestellt wird.</p>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 1 supplement","pages":"91 - 100"},"PeriodicalIF":1.4,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01481-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140116095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-12DOI: 10.1007/s00003-024-01491-y
Laura Schmitz, Miriam Kemnade, Marcus Mergenthaler
Several studies have shown that farmers' perceptions of livestock production are influenced by a variety of factors. This paper examines farmers' perceptions of livestock production and animal welfare based on survey data. It was found that farmers see themselves as having the greatest responsibility for ensuring animal welfare, for which they would like to see a stable political framework and support from society. Furthermore, organic livestock production (except for dairy and suckler cows) got better ratings than conventional livestock production. The aspects perceived as the most important for animal welfare varied according to species and type of farming. This survey design approach indents to serve as a long-term tool for the Federal Ministry of Agriculture, in order to monitor the situation in livestock production.
{"title":"Ausgewählte Sichtweisen von Landwirtinnen und Landwirten auf die Nutztierhaltung in Deutschland","authors":"Laura Schmitz, Miriam Kemnade, Marcus Mergenthaler","doi":"10.1007/s00003-024-01491-y","DOIUrl":"10.1007/s00003-024-01491-y","url":null,"abstract":"<div><p>Several studies have shown that farmers' perceptions of livestock production are influenced by a variety of factors. This paper examines farmers' perceptions of livestock production and animal welfare based on survey data. It was found that farmers see themselves as having the greatest responsibility for ensuring animal welfare, for which they would like to see a stable political framework and support from society. Furthermore, organic livestock production (except for dairy and suckler cows) got better ratings than conventional livestock production. The aspects perceived as the most important for animal welfare varied according to species and type of farming. This survey design approach indents to serve as a long-term tool for the Federal Ministry of Agriculture, in order to monitor the situation in livestock production.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 1 supplement","pages":"69 - 74"},"PeriodicalIF":1.4,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01491-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140115876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-05DOI: 10.1007/s00003-024-01498-5
Annika Ehmke, Jens Karl Wegener, Andreas Melfsen, Eberhard Hartung
When approving plant protection products, exposure data are required for risk analysis. Exposure data can be collected for various exposure pathways, such as dermal, inhalation or ground sediment. For measuring exposures, pyranine, a fluorescent dye, and a collector can be used. However, the choice of collector material depends on the specific exposure pathway. This study aims to determine the most suitable collector, in combination with the tracer pyranine, for recording exposure through different pathways in practical trials.
Seven different collectors (Tyvek®, labels, plastic patches, paper patches, nylon filters, fibreglass filters, petri dishes) were subjected to laboratory and field tests to assess various quality parameters. Blank values, recovery rates, storability, and fluorescence degradation under UV-radiation were measured. Based on the results, a matrix was created summarizing which collector might be best suited to capture each exposure pathway. Almost all collectors demonstrated high recovery rates (Tyvek® 100%; labels 100%; plastic patches 100%; paper patches 100%; nylon filters 95%; fiberglass filters 60.9%) as well as good storability. Furthermore, all plastic-based collectors (labels, Tyvek®, plastic patches, petri dishes) showed a very good recovery rate above 95% when exposed to UV-radiation. However, nylon filters were not suitable for utilization under field conditions due to the rapid degradation of fluorescence under UV-radiation (recovery rate: 20–56%). Nevertheless, nylon filters showed stable recoveries under protected conditions and can be used to assess inhalation exposures under these conditions. Fibreglass filters, with recovery rates of 84–86%, were well-suited for testing inhalative exposures outdoors when a correction factor was applied. Tyvek® was the most suitable material for detecting total dermal exposure under field conditions. This is due to its quality characteristics and availability in a variety of sizes. Finally, petri dishes were ideal for collecting ground sediments.
{"title":"Optimizing exposure data collection for plant protection products: identifying ideal collectors with the fluorescent dye pyranine","authors":"Annika Ehmke, Jens Karl Wegener, Andreas Melfsen, Eberhard Hartung","doi":"10.1007/s00003-024-01498-5","DOIUrl":"10.1007/s00003-024-01498-5","url":null,"abstract":"<div><p>When approving plant protection products, exposure data are required for risk analysis. Exposure data can be collected for various exposure pathways, such as dermal, inhalation or ground sediment. For measuring exposures, pyranine, a fluorescent dye, and a collector can be used. However, the choice of collector material depends on the specific exposure pathway. This study aims to determine the most suitable collector, in combination with the tracer pyranine, for recording exposure through different pathways in practical trials.</p><p>Seven different collectors (Tyvek<sup>®</sup>, labels, plastic patches, paper patches, nylon filters, fibreglass filters, petri dishes) were subjected to laboratory and field tests to assess various quality parameters. Blank values, recovery rates, storability, and fluorescence degradation under UV-radiation were measured. Based on the results, a matrix was created summarizing which collector might be best suited to capture each exposure pathway. Almost all collectors demonstrated high recovery rates (Tyvek<sup>®</sup> 100%; labels 100%; plastic patches 100%; paper patches 100%; nylon filters 95%; fiberglass filters 60.9%) as well as good storability. Furthermore, all plastic-based collectors (labels, Tyvek<sup>®</sup>, plastic patches, petri dishes) showed a very good recovery rate above 95% when exposed to UV-radiation. However, nylon filters were not suitable for utilization under field conditions due to the rapid degradation of fluorescence under UV-radiation (recovery rate: 20–56%). Nevertheless, nylon filters showed stable recoveries under protected conditions and can be used to assess inhalation exposures under these conditions. Fibreglass filters, with recovery rates of 84–86%, were well-suited for testing inhalative exposures outdoors when a correction factor was applied. Tyvek<sup>®</sup> was the most suitable material for detecting total dermal exposure under field conditions. This is due to its quality characteristics and availability in a variety of sizes. Finally, petri dishes were ideal for collecting ground sediments.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 2","pages":"245 - 253"},"PeriodicalIF":1.4,"publicationDate":"2024-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01498-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140037726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-24DOI: 10.1007/s00003-024-01499-4
Christopher Weidner, René Köppel, Regina Freyer, Petra Richl, Kathrin Lieske, Joachim Mankertz, Hans-Ulrich Waiblinger
The diversity of genetically modified plants used for food and feed is increasing worldwide. For the detection and control of these products, efficient and reliable analytical tools are a prerequisite. This can be done by screening for specific DNA-elements and constructs characteristic of transgenic plants. In the past, numerous methods have already been published. However, several genetically modified plants are not covered by common screening methods. Here, a new qualitative triplex real-time polymerase chain reaction (PCR) method is presented, detecting two transgene flanking sequences and the transition between the Cassava Vein Mosaic Virus Promotor (P-CsVMV) and the phosphinothricin-N-acetyltransferase (pat) gene. These sequences are present in several transgenic plants and therefore, the described triplex method can be used as a screening tool to guide further analysis and increase the efficiency of the analysis strategy for GMO detection. The method is characterized by high specificity, sensitivity and robustness and is provided as a ring-trial validated method in the Official Collection of Methods according to the German Food and Feed Act.
全球范围内用于食品和饲料的转基因植物日益多样化。要检测和控制这些产品,高效可靠的分析工具是先决条件。这可以通过筛选转基因植物特有的 DNA 元素和构建体来实现。过去,已经有许多方法发表。然而,一些转基因植物并不在常用筛选方法的范围内。本文介绍了一种新的定性三重实时聚合酶链反应(PCR)方法,该方法可检测两个转基因侧翼序列以及木薯花叶病毒启动子(P-CsVMV)和膦丝菌素-N-乙酰转移酶(pat)基因之间的过渡。这些序列存在于几种转基因植物中,因此,所述三重法可用作筛选工具,指导进一步的分析,并提高转基因生物检测分析策略的效率。该方法具有特异性高、灵敏度高和稳健性强的特点,已作为环试验证方法列入《德国食品和饲料法官方方法集》。
{"title":"Development and validation of a multiplex real-time PCR method for screening genetically modified plants","authors":"Christopher Weidner, René Köppel, Regina Freyer, Petra Richl, Kathrin Lieske, Joachim Mankertz, Hans-Ulrich Waiblinger","doi":"10.1007/s00003-024-01499-4","DOIUrl":"10.1007/s00003-024-01499-4","url":null,"abstract":"<div><p>The diversity of genetically modified plants used for food and feed is increasing worldwide. For the detection and control of these products, efficient and reliable analytical tools are a prerequisite. This can be done by screening for specific DNA-elements and constructs characteristic of transgenic plants. In the past, numerous methods have already been published. However, several genetically modified plants are not covered by common screening methods. Here, a new qualitative triplex real-time polymerase chain reaction (PCR) method is presented, detecting two transgene flanking sequences and the transition between the Cassava Vein Mosaic Virus Promotor (P-CsVMV) and the phosphinothricin-N-acetyltransferase (pat) gene. These sequences are present in several transgenic plants and therefore, the described triplex method can be used as a screening tool to guide further analysis and increase the efficiency of the analysis strategy for GMO detection. The method is characterized by high specificity, sensitivity and robustness and is provided as a ring-trial validated method in the Official Collection of Methods according to the German Food and Feed Act.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 2","pages":"165 - 174"},"PeriodicalIF":1.4,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01499-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139951884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-16DOI: 10.1007/s00003-024-01478-9
Sophie Hedges, Ludovic Pelligand, Liwei Chen, Kelyn Seow, Thuy Thi Hoang, Huong Quynh Luu, Son Thi Thanh Dang, Ngoc Thi Pham, Hoa Thi Thanh Pham, Yeong Cheng Cheah, Yulan Wang, Dominique Hurtaud-Pessel, Anne Conan, Guillaume Fournié, Damer Blake, Fiona Tomley, Patricia L. Conway
Antimicrobials used in chicken farming for therapeutic and/or prophylactic purposes may result in unacceptable levels of edible residues, if withdrawal periods are not respected. To evaluate the risk in Vietnam, we validated an analytical method to detect antimicrobial residues from chicken meat samples and carried out a pilot cross-sectional study to identify optimal sampling strategies. A total of 45 raw meat samples were collected from 4 markets, 1 slaughterhouse and 4 farms (5 per site) in Northern Vietnam, between March and April 2021. Farmers were asked about antimicrobials used during sampled production cycles (5 chickens sampled per batch). Samples were analysed using liquid chromatography-tandem mass spectrometry for the presence of 68 antimicrobials at a pre-defined validation concentration. 7 compounds were identified from 4 classes (tetracyclines, sulphonamides, macrolides, and fluoroquinolones). In markets, where the source of sampled chickens was unknown, a diverse pool of residual antimicrobials was detected in 20% (4/20) of the meat samples. No residues were detected in samples from the slaughterhouse. No residues were detected in chickens from the one farm that reported using antimicrobials, whereas sulfadimethoxine, doxycycline and tilmicosin residues were identified from the other 3 farms reporting no antimicrobial use. The probability of detecting antimicrobial residues present in a flock based on sampling a single chicken was estimated at 0.93 (highest density interval 0.735–0.997). The preliminary results suggest a disparity between farmers’ reports on antimicrobial drug use and actual usage, and that the analysis of a single sample per farm has a high probability of detecting antimicrobial residues, if present.
{"title":"Antimicrobial residues in meat from chickens in Northeast Vietnam: analytical validation and pilot study for sampling optimisation","authors":"Sophie Hedges, Ludovic Pelligand, Liwei Chen, Kelyn Seow, Thuy Thi Hoang, Huong Quynh Luu, Son Thi Thanh Dang, Ngoc Thi Pham, Hoa Thi Thanh Pham, Yeong Cheng Cheah, Yulan Wang, Dominique Hurtaud-Pessel, Anne Conan, Guillaume Fournié, Damer Blake, Fiona Tomley, Patricia L. Conway","doi":"10.1007/s00003-024-01478-9","DOIUrl":"10.1007/s00003-024-01478-9","url":null,"abstract":"<div><p>Antimicrobials used in chicken farming for therapeutic and/or prophylactic purposes may result in unacceptable levels of edible residues, if withdrawal periods are not respected. To evaluate the risk in Vietnam, we validated an analytical method to detect antimicrobial residues from chicken meat samples and carried out a pilot cross-sectional study to identify optimal sampling strategies. A total of 45 raw meat samples were collected from 4 markets, 1 slaughterhouse and 4 farms (5 per site) in Northern Vietnam, between March and April 2021. Farmers were asked about antimicrobials used during sampled production cycles (5 chickens sampled per batch). Samples were analysed using liquid chromatography-tandem mass spectrometry for the presence of 68 antimicrobials at a pre-defined validation concentration. 7 compounds were identified from 4 classes (tetracyclines, sulphonamides, macrolides, and fluoroquinolones). In markets, where the source of sampled chickens was unknown, a diverse pool of residual antimicrobials was detected in 20% (4/20) of the meat samples. No residues were detected in samples from the slaughterhouse. No residues were detected in chickens from the one farm that reported using antimicrobials, whereas sulfadimethoxine, doxycycline and tilmicosin residues were identified from the other 3 farms reporting no antimicrobial use. The probability of detecting antimicrobial residues present in a flock based on sampling a single chicken was estimated at 0.93 (highest density interval 0.735–0.997). The preliminary results suggest a disparity between farmers’ reports on antimicrobial drug use and actual usage, and that the analysis of a single sample per farm has a high probability of detecting antimicrobial residues, if present.</p></div>","PeriodicalId":622,"journal":{"name":"Journal of Consumer Protection and Food Safety","volume":"19 2","pages":"225 - 234"},"PeriodicalIF":1.4,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00003-024-01478-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139755507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}