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Guideline for the verification of digital PCR methods in analytical GMO testing 转基因生物分析检测中数字 PCR 方法验证指南
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-25 DOI: 10.1007/s00003-024-01516-6
Christopher Weidner, Jakob Frenzel, Daniela Bartsch, Hans-Ulrich Waiblinger, Joachim Mankertz

The international working group “Development of methods for identification of foodstuffs produced by means of genetic engineering techniques” was established pursuant to § 64 of the German Food and Feed Code (LFGB). It has developed a guideline for implementing and verifying digital PCR methods previously validated as real-time PCR techniques. While event-specific methods in testing of genetically modified organisms (GMO) have traditionally relied on real-time PCR, new digital PCR technologies offer significant advantages and therefore, leading to an increased use by control laboratories. This guideline provides practical recommendations for transferring the real-time PCR to digital PCR and for verifying the digital PCR method. The guideline is applicable to analysis of GMO in food, feed and seed and is freely available on the website of the Federal Office of Consumer Protection and Food Safety (BVL).

根据《德国食品和饲料法典》(LFGB)第 64 条,成立了 "基因工程技术食品鉴别方法开发 "国际工作组。该工作组为实施和验证先前作为实时 PCR 技术验证的数字 PCR 方法制定了指导原则。虽然转基因生物 (GMO) 检测中的特定事件检测方法传统上依赖于实时 PCR,但新的数字 PCR 技术具有显著优势,因此控制实验室越来越多地使用这种方法。本指南提供了将实时 PCR 转为数字 PCR 以及验证数字 PCR 方法的实用建议。该指南适用于食品、饲料和种子中转基因生物的分析,可在联邦消费者保护和食品安全办公室 (BVL) 的网站上免费获取。
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引用次数: 0
Comprehensive analysis of 19 cannabinoids in commercial CBD oils: concentrations, profiles, and safety implications 全面分析商用 CBD 油中的 19 种大麻素:浓度、特征和安全影响
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-20 DOI: 10.1007/s00003-024-01513-9
Niklas Lindekamp, Stefan Weigel, Benjamin Sachse, Bernd Schäfer, Sascha Rohn, Nadja Triesch

Nineteen cannabinoids, including Δ9-THC (tetrahydrocannabinol) and CBD (cannabidiol), were quantified in 22 CBD oils, 2 CBG (cannabigerol) oils, and 2 CBN (cannabinol) oils, marketed as food supplements, aroma oils, or cosmetic preparations. Analyses were conducted using high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS). The declared concentrations of CBD (or CBG, CBN) in the oils ranged from 2.5 to 20%. Actual concentrations compared to declared concentrations ranged from 81 to 226%. CBD concentrations of up to 9 mg per drop were found. Δ9-THC was detected in 20 of 26 samples, with concentrations ranging from 5 to 1576 mg/kg (mean = 536 mg/kg). Considering the highest daily intake suggested by one manufacturer (20 drops) and a body weight of 70 kg, the measured Δ9-THC concentration in 50% (n = 13) of the products would exceed the acute reference dose (ARfD) of 1 µg/kg body weight (bw) derived for Δ9-THC by the European Food Safety Authority (EFSA). For 7 samples, only 2 drops of oil would be sufficient to exceed the ARfD of Δ9-THC.

对 22 种 CBD 油、2 种 CBG(大麻酚)油和 2 种 CBN(大麻酚)油中的 19 种大麻素(包括 Δ9-THC(四氢大麻酚)和 CBD(大麻二酚))进行了定量分析,这些油在市场上作为食品补充剂、芳香油或化妆品制剂销售。分析采用高效液相色谱-串联质谱法(HPLC-MS/MS)进行。精油中 CBD(或 CBG、CBN)的申报浓度介于 2.5% 和 20% 之间。与申报浓度相比,实际浓度从 81% 到 226% 不等。发现每滴 CBD 的浓度高达 9 毫克。在 26 个样本中的 20 个样本中检测到了Δ9-四氢大麻酚,浓度从 5 毫克/千克到 1576 毫克/千克不等(平均值 = 536 毫克/千克)。考虑到一家生产商建议的最高日摄入量(20 滴)和 70 千克的体重,50% 的产品(n = 13)中测得的 Δ9-THC 浓度将超过欧洲食品安全局(EFSA)为 Δ9-THC 制定的急性参考剂量(ARfD),即 1 微克/千克体重。在 7 个样本中,只需 2 滴油就足以超过 Δ9-THC 的急性参考剂量。
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引用次数: 0
RNA-based crop protection: potential, challenges, and practical examples 基于 RNA 的作物保护:潜力、挑战和实例
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-12 DOI: 10.1007/s00003-024-01507-7
Aline Koch
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引用次数: 0
Amtliche Untersuchungsergebnisse von Einweg-E-Zigaretten aus dem Jahr 2022 in Deutschland 德国 2022 年起对一次性电子烟的官方测试结果
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-06-09 DOI: 10.1007/s00003-024-01510-y
Frauke Kirsch, Eva-Maria Kröner, Robert Lange, Julia Niemeyer, Miriam Laible, Julia Seidel, Katrin Paulus, Annette Stephani, Sandra Tamosaite, Katja Dünnebier

Gesetzliche Regelungen legen fest, welchen Vorgaben E-Zigaretten entsprechen müssen, damit sie rechtmäßig in Verkehr gebracht werden können. Diese Regeln gelten auch für Einweg-E-Zigaretten, deren Nutzung insbesondere unter Jugendlichen verbreitet ist. Im Rahmen amtlicher Untersuchungen in verschiedenen deutschen Bundesländern wurden im Jahr 2022 bei 250 Einweg-E-Zigaretten einige dieser rechtlichen Vorgaben mit Blick auf die Verkehrsfähigkeit untersucht. Neben der Kennzeichnung (Nikotingehalt, Füllvolumen/Zuganzahl, Nikotinwarnhinweise, Beipackzettel, Gefahrstoffkennzeichnung) wurden auch die Produktsicherheit (Kindersicherung) sowie Aspekte der Täuschung von Verbraucherinnen und Verbrauchern und die Erfüllung der Meldepflicht im einheitlichen elektronischen Portal der EU (EU-CEG) (https://health.ec.europa.eu/eu-common-entry-gate-eu-ceg/providing-information-tobacco-products-e-cigarettes-and-refill-containers-eu-common-entry-gate-eu_de%20%20Zugriff:%2013.05.2024) betrachtet. Keine der untersuchten Einweg-E-Zigaretten erwies sich als verkehrsfähig, da mindestens bei einem beanstandungsfähigen Kriterium erhebliche Mängel festgestellt wurden.

法律法规规定,电子烟必须满足哪些要求才能合法投放市场。这些规定也适用于一次性电子烟,尤其是年轻人广泛使用的一次性电子烟。作为 2022 年德国各联邦州官方调查的一部分,对 250 支一次性电子烟的可销售性进行了审查。除了标签(尼古丁含量、填充量/吸食次数、尼古丁警告、包装插页、有害物质标签)、产品安全(儿童安全)外,还检查了欺骗消费者的问题以及遵守欧盟单一电子门户网站(EU-CEG)(https://health.ec.europa.eu/eu-common-entry-gate-eu-ceg/providing-information-tobacco-products-e-cigarettes-and-refill-containers-eu-common-entry-gate-eu_de%20%20Zugriff:%2013.05.2024)报告义务的情况。所分析的一次性电子烟中没有一种被证明是适销对路的,因为至少在一项令人反感的标准中发现了重大缺陷。
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引用次数: 0
Correction: 121. ALS-Sitzung 更正:第 121 届 ALS 会议
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-20 DOI: 10.1007/s00003-024-01504-w
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引用次数: 0
Upcoming events 即将举行的活动
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-08 DOI: 10.1007/s00003-024-01508-6
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引用次数: 0
Malawi’s ‘anything goes’ food market: weak regulations on nutrition and health claims 马拉维 "随心所欲 "的食品市场:营养和健康声明监管薄弱
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-27 DOI: 10.1007/s00003-024-01509-5
Rutendo Bell, Aggrey Pemba Gama, Limbikani Matumba

Regulations on nutrition and health claims are useful to protect consumers from deceptive practices of unscrupulous food manufacturers. Consumer protection is best achieved when relevant regulations are available and enforced. So far, no study has analyzed the robustness of regulations on nutrition and health claims in Malawi. In the present study, Malawi’s regulation was compared with Codex Alimentarius as well as European, U.S American, and the Republic of South Africa’s (RSA) regulations on nutrition and health claims. The study revealed that Malawi does not have regulations or guidelines on health claims and therefore, any health claim can be made. Likewise, Malawi’s regulation on nutrition claims was found to be the least demanding and less elaborate. Out of 11 nutrition claims related requirements, the Malawi regulation satisfied only 3. The lack of comprehensive nutrition and health claims regulations in Malawi may give an opportunity to dishonest manufacturers to mislead and deceive consumers. False claims can also derail government’s efforts towards addressing some nutrient deficiencies through food fortification programs. Currently, it is difficult to effectively regulate nutrition and health claims in Malawi. Therefore, the findings are a wake-up call for Malawi and other countries that may be in a similar situation to take the necessary remedial actions.

有关营养和健康声明的法规有助于保护消费者免受不良食品制造商欺骗行为的侵害。有了相关法规并加以执行,才能最好地保护消费者。迄今为止,还没有研究分析过马拉维营养与健康声明法规的健全性。在本研究中,马拉维的法规与《食品法典》以及欧洲、美国和南非共和国(RSA)的营养与健康声明法规进行了比较。研究表明,马拉维没有关于健康声称的法规或准则,因此可以提出任何健康声称。同样,马拉维关于营养声称的规定也是要求最低、最不详细的。在 11 项与营养声称有关的要求中,马拉维的法规只满足了 3 项要求。马拉维缺乏全面的营养和健康声称法规可能会给不诚实的制造商提供误导和欺骗消费者的机会。虚假声明还可能破坏政府通过食品营养强化计划解决某些营养素缺乏问题的努力。目前,马拉维很难对营养和健康声明进行有效监管。因此,这些发现为马拉维和其他可能面临类似情况的国家敲响了警钟,要求它们采取必要的补救措施。
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引用次数: 0
Efficiency of working coveralls and chemical resistant gloves in reducing operator exposure to pesticides 工作服和防化手套在减少操作员接触杀虫剂方面的效率
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-25 DOI: 10.1007/s00003-024-01506-8
Christian J. Kuster, Felix M. Kluxen, Edgars Felkers, Neil Morgan, Nicola J. Hewitt, Julien Durand-Reville

Personal Protective Equipment (PPE) is used to reduce exposure when working with chemicals. For pesticides, exposure scenarios comprise mixing and loading (M&L) and application. The exposure prediction model recommended by European regulatory agencies for operator risk assessments—the Agricultural Operator Exposure Model (AOEM) – is based on 48 operator exposure studies involving over 500 professional operators from 10 European countries in typical working situations to assess exposure under realistic field conditions. We used the AOEM data to assess the efficiency of exposure reduction resulting from wearing chemical-resistant nitrile gloves and non-certified working coveralls during M&L (tank and backpack sprayer), applications using groundboom and airblast spraying, and outdoor and indoor (greenhouse) applications using handheld sprayers. All studies indicated consistent and high exposure reduction > 90% for gloves and non-certified coveralls across all investigated scenarios. Exceptions were almost exclusively observed when an operator incorrectly used the PPE. The mean exposure reduction from all operators, including those that used PPE incorrectly, was 95.0% for gloves and 96.4% for polyester/cotton working coveralls during M&L, and 91.1% for gloves and 94.9% for non-certified coveralls during all application scenarios. This analysis confirms the essential role of PPE and non-certified working coveralls in reducing operator exposure to pesticides. Outliers indicate that operators need to be properly trained and adhere to label instructions to apply pesticides according to good agricultural practices.

个人防护设备(PPE)用于减少接触化学品的机会。对于农药来说,暴露情景包括混合和装载(M&L)以及施用。欧洲监管机构推荐用于操作员风险评估的接触预测模型--农业操作员接触模型(AOEM)--是基于 48 项操作员接触研究,涉及来自 10 个欧洲国家的 500 多名专业操作员在典型工作环境下的接触情况,以评估现实田间条件下的接触情况。我们利用 AOEM 数据评估了在使用 M&L(罐式和背负式喷雾器)、地面喷洒和喷气喷洒以及使用手持式喷雾器进行室外和室内(温室)喷洒时,佩戴耐化学品丁腈手套和非认证工作服所能降低的接触效率。所有研究都表明,在所有调查场景中,手套和非认证工作服的暴露减少率一致且较高,均为 90%。几乎只有在操作员错误使用个人防护设备时才会出现例外情况。所有操作人员(包括错误使用个人防护设备的操作人员)的平均接触减少率为:在 M&L 期间,手套为 95.0%,涤纶/棉工作服为 96.4%;在所有应用场景中,手套为 91.1%,非认证工作服为 94.9%。这项分析证实了个人防护设备和非认证工作服在减少操作员接触农药方面的重要作用。异常值表明,操作人员需要接受适当的培训,并遵守标签说明,按照良好农业规范施用农药。
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引用次数: 0
SocialLab: Durch Forschung und Zusammenarbeit hin zu einer nachhaltigen landwirtschaftlichen Nutztierhaltung 社会实验室:通过研究与合作实现可持续畜牧业
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-23 DOI: 10.1007/s00003-024-01484-x
Marie von Meyer-Höfer, Monika Hartmann, Peter Kenning, Jörg Luy, Marcus Mergenthaler, Jutta Roosen, Achim Spiller, Inken Christoph-Schulz

Das Projekt SocialLab, das im Jahr 2012 ins Leben gerufen wurde, zielt darauf ab, die Notwendigkeit von Verbesserungen in landwirtschaftlichen Nutztierhaltungssystemen, sowohl aus wissenschaftlicher als auch aus gesellschaftlicher Sicht anzugehen. Denn falls umfassende Veränderungen hin zu mehr Nachhaltigkeit bei der Produktion tierischer Lebensmittel nicht erreicht werden, besteht die Gefahr für die gesamte Branche, dass die gesellschaftliche Akzeptanz für tierische Lebensmittel verloren geht. Das Projekt verwendet einen multidisziplinären und partizipativen Ansatz und bezieht Verbraucherinnen und Verbraucher, landwirtschaftliche Betriebe, den Einzelhandel und Interessenvertretungen ein, um Erkenntnisse zu sammeln und Empfehlungen für Politik, Praxis und Forschung zu formulieren. Die Ergebnisse von SocialLab II sind in der gesamten landwirtschaftlichen Wertschöpfungskette von Bedeutung: Landwirtschaftliche Betriebe erhalten Einblicke in markt- und gesellschaftliche Anforderungen, was ihr Vertrauen in die Gestaltung neuer Tierhaltungssysteme stärken könnte. Darüber hinaus bietet SocialLab II ein Monitoring-Tool für politische Entscheidungsträger, um Veränderungen in der gesellschaftlichen Wahrnehmung und Akzeptanz der Nutztierhaltung zu messen. Dieses Tool ermöglicht auch eine Anleitung zur Gestaltung des rechtlichen Rahmens für die Nutztierhaltung. Der Einzelhandelssektor kann von den Einblicken zum Einfluss von Preisstrategien und Tierwohl-Labels auf das Kaufverhalten der  Verbraucherinnen und Verbraucher profitieren. Zusammengefasst bietet die Infrastruktur von SocialLab die Gelegenheit für eine evidenzbasierte, partizipative, multidisziplinäre und situationsflexible Beteiligung an der Transformation der Nutztierhaltung in Deutschland.

社会实验室(SocialLab)项目于 2012 年启动,旨在从科学和社会两个角度满足改善畜牧业系统的需求。如果不能在动物性食品生产中实现更加可持续的全面变革,整个行业就有可能失去社会对动物性食品的认可。该项目采用多学科和参与式方法,让消费者、农场、零售商和利益相关组织参与其中,以收集见解并为政策、实践和研究制定建议。社会实验室 II 的成果与整个农业价值链相关:农场深入了解了市场和社会的需求,这可以增强他们设计新畜牧系统的信心。此外,SocialLab II 还为决策者提供了一个监测工具,用于衡量社会对畜牧业的看法和接受程度的变化。该工具还可为畜牧业法律框架的设计提供指导。零售部门可以从定价策略和动物福利标签对消费者购买行为的影响中获益。总之,SocialLab 基础设施为以证据为基础的、参与性的、多学科的和灵活的参与德国畜牧业转型提供了机会。
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引用次数: 0
Arbeitspaket 1: Monitoring der gesellschaftlichen Akzeptanz der Nutztierhaltung 工作包 1:监测社会对畜牧业的接受程度
IF 1.4 3区 农林科学 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-04-22 DOI: 10.1007/s00003-024-01485-w
Inken Christoph-Schulz, Ivica Faletar, Monika Hartmann, Peter Kenning, Jörg Luy, Marcus Mergenthaler, Jutta Roosen, Achim Spiller, Marie von Meyer-Höfer

The aim of the first work package (WP 1) of SocialLab II was to establish a monitoring system for framework conditions that influence the development of social criticism and acceptance of livestock farming. Based on the results of SocialLab I (Christoph-Schulz et al., J Consum Prot Food Saf 13:145–236, 2018), the aim was to establish a long-term longitudinal study. The monitoring was carried out, set up and tested online with a total of 4 survey waves and representative samples of consumers. In addition, farmers and retailers were surveyed. This enabled the development and validation of a survey tool that will allow the German Ministry of Agriculture to conduct surveys at regular intervals on the following topics that are important in the societal debate on the further development of livestock farming.

社会实验室 II 的第一个工作包(WP 1)旨在建立一个监测系统,监测影响社会对畜牧业发展的批评和接受程度的框架条件。基于 SocialLab I 的成果(Christoph-Schulz 等人,J Consum Prot Food Saf 13:145-236,2018 年),目标是建立一个长期纵向研究。监测工作在网上进行、设置和测试,共进行了 4 波调查,并抽取了具有代表性的消费者样本。此外,还对农民和零售商进行了调查。这样就开发并验证了一种调查工具,使德国农业部能够定期就以下对社会讨论畜牧业进一步发展具有重要意义的主题进行调查。
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引用次数: 0
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Journal of Consumer Protection and Food Safety
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