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Optimizing the Operation of Fermenters 优化发酵罐的运行
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0056
D. Drăcea, A. Tronac, Sebastian Mustata
Abstract World energy systems development is based on the use of renewable sources, their valorisation benefits of technical and economical stimulating actions. The main disadvantage is related to the low efficiency caused by low energy density and high value investments. The cost of renewable energy is higher than energy coming from conventional sources. Reducing the costs of producing unconventional energy can be achieved by integrating economic and social activities into the system, providing futher benefits. From the potential point of view, it is estimated that biomass occupies the first place in a ranking of unconventional energies. Biomass boosted by anaerobic fermentation with biogas production and cogeneration can provide "peak" energy production and can balance energy systems. Waste utilized by these processes involves reducing environmental impacts by diminushing managed volumes and efficient control. In the present paper are characterized by calculations aspects of the exploitation and elements regarding the optimization of some high-capacity fermenters of a wastewater treatment system.
世界能源系统的发展是基于可再生能源的使用,其增值效益的技术和经济刺激行动。主要的缺点是低能量密度和高价值投资导致的低效率。可再生能源的成本高于传统能源。通过将经济和社会活动整合到系统中,可以降低非常规能源的生产成本,从而带来进一步的效益。从潜力的角度来看,估计生物质能在非常规能源的排名中占据首位。由厌氧发酵与沼气生产和热电联产推动的生物质可以提供“峰值”能源生产,并可以平衡能源系统。这些过程利用的废物涉及通过减少管理的数量和有效控制来减少对环境的影响。本文的特点是计算方面的开发和要素有关的一些大容量发酵罐的废水处理系统的优化。
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引用次数: 0
Variation of Some Antioxidants Content in Rosa Canina L. Fruit During Storage 犬蔷薇果实贮藏过程中部分抗氧化剂含量的变化
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0082
Evelina Gherghina, D. Balan, G. Luță, F. Israel-Roming
Abstract Rosa canina L. fruit have been used internally as tea for treatment of viral infections and disorders of the kidneys and urinary tract. Also, it was reported that the R. canina fruit, with its high ascorbic acid, phenolics and flavonoids contents, have antioxidant, antimutagenic and anticarcinogenic effects. Antioxidants neutralize free radicals, which cause oxidative damage to lipids, proteins, and nucleic acids and thus protect the organism. Research studies conducted on this topic have shown that natural antioxidants are involved in protection against many diseases: cancer, cardiovascular diseases and osteoporosis. Different processing and storage methods of the fruit could affect the bioactive nutrients while preserving the antioxidant capacity of them is very important. The aim of this study was to evaluate the effect of storage in a dry or frozen state on the antioxidants content of R. canina fruit. The evaluation involved determination of carotenes, ascorbic acid and total polyphenols using spectrophotometrically methods. Analysis performed on the R. canina fruit showed significant changes in the antioxidants content in fresh, dried and frozen fruit during storage.
摘要犬蔷薇果已被用作茶内服治疗病毒感染和疾病的肾脏和泌尿道。此外,据报道,牛蒡子含有较高的抗坏血酸、酚类物质和类黄酮,具有抗氧化、抗诱变和抗癌作用。抗氧化剂中和自由基,自由基会对脂质、蛋白质和核酸造成氧化损伤,从而保护生物体。关于这一主题的研究表明,天然抗氧化剂可以预防许多疾病:癌症、心血管疾病和骨质疏松症。不同的加工和贮藏方法会影响果实中生物活性营养素的含量,而保持其抗氧化能力是非常重要的。本研究的目的是评价在干燥和冷冻状态下储存对牛蒡果实抗氧化剂含量的影响。评价涉及用分光光度法测定胡萝卜素、抗坏血酸和总多酚。对牛蒡果实的分析表明,在贮藏过程中,新鲜、干燥和冷冻果实的抗氧化剂含量发生了显著变化。
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引用次数: 0
Comparative Assessment of the Analytical Parameters in Ascorbic Acid and Sulphite Assay at a Spectrographic Carbon Working Electrode 碳工作电极上抗坏血酸和亚硫酸盐分析参数的比较研究
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0072
A. Pisoschi, A. Pop
Abstract The aim of this study is the comparative investigation of spectrographic carbon electrode’s viability as working electrode, in ascorbic acid and sulphite asssessment. Cyclic voltammetry involves a linear sweeping of the potential, the analytical signal being represented by the anodic oxidation /cathodic reduction peak of the analyte. For both analytes, the electro-oxidation resulted in an anodic peak, correlable with ascorbic acid / sulphite concentration. The analytical range of linear response corresponded to 0.07 - 10 mM for ascorbic acid and to 15.5 mg/L - 4 g/L for sulphite. The relative standard deviation RSD (%) was 2.71 % for ascorbic acid and 2.88 % for sulphite. The sensitivities, given by the slopes of the calibration graphs were 88.88 μA/mmole/L for ascorbic acid and 477.37 μA/g/L for sulphite.
摘要本研究的目的是比较研究光谱碳电极作为工作电极在抗坏血酸和亚硫酸盐评价中的可行性。循环伏安法涉及电位的线性扫描,分析信号由被分析物的阳极氧化/阴极还原峰表示。对于这两种分析物,电氧化导致阳极峰,与抗坏血酸/亚硫酸盐浓度相关。对抗坏血酸的线性响应范围为0.07 ~ 10 mM,对亚硫酸盐的线性响应范围为15.5 ~ 4 g/L。抗坏血酸和亚硫酸盐的相对标准偏差RSD(%)分别为2.71%和2.88%。校正曲线斜率显示,抗坏血酸的灵敏度为88.88 μA/ mol /L,亚硫酸盐的灵敏度为477.37 μA/g/L。
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引用次数: 1
Bioconversion of Oilseeds into Bioproducts for Cultivated Plants Nutrition and Protection 油籽转化为栽培植物营养与保护生物制品的研究
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0090
M. Popescu, F. Oancea, E. Radu, Mălina Deșliu-Avram, C. Cornea
Abstract Natural by-products from vegetable oil industries and spent edible oils from domestic or public food spaces should be recycled to obtain new added value products. Present paper proposed a technical solution for complete valorisation of inedible oilseeds or spent edible oils into bioproducts for nutrition and protection of plants cultivated in conservative organic agrosystems. Pressed cakes resulted from mechanical cold extraction of mustard oil contain residual oil and bioactive compounds which were released using an enzymatic cocktail 1:1 cellulase with proteases coupled with azeotropic solvents into a single Soxhlet extractor. From mustard meal, a solid fraction with glycerol derivatives of fatty acids (56.23% oleate and 17.47% linoleate) decanted from syrup (41.78% xylopyranoside and 48.48% trilinolein) and from mustard cake (76.44% linoleate) in the supernatant, the same oligosaccharide (29.64%) and proteinates (30.18%) in the solid fraction. The total extract was simultaneously concentrated and converted into a bioactive potassium salt emulsion able to encapsulate insectofungicidal natural compounds as bioproducts with agronomical applications.
植物油工业的天然副产品和家庭或公共食品场所的废食用油应回收利用,以获得新的附加价值产品。本文提出了一种将不可食用油籽或废食用油完全转化为生物制品的技术方案,用于保护保守有机农业系统中栽培的植物的营养和保护。榨油机冷萃取得到的榨油饼含有残油和生物活性化合物,这些化合物是用酶鸡尾酒1:1纤维素酶与蛋白酶和共沸溶剂耦合到单个索氏萃取器中释放的。从榨菜粕中,从糖浆(41.78%木pyrano苷和48.48% trilinolein)和榨菜饼(76.44%亚油酸)中分离出含有脂肪酸(56.23%油酸和17.47%亚油酸)的甘油衍生物的固体馏分,在固体馏分中得到相同的低聚糖(29.64%)和蛋白质(30.18%)。同时将总提取物浓缩并转化为具有生物活性的钾盐乳液,可包封杀虫真菌的天然化合物作为农用生物制品。
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引用次数: 0
Evaluation of Fatty Acids Composition of Some Food Samples by Using GC-MS and NMR Techniques 用气相色谱-质谱和核磁共振技术评价食品中脂肪酸组成
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0086
A. L. Mihai, M. Negoiță, A. Adascălului, V. Ionescu, N. Belc
Abstract The purpose of this paper was to compare the composition (weight % of total identified FA) in saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids from 3 food matrices (sunflower oil, palm oil and lard) by 2 different techniques, gas chromatography - mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR). For GC-MS technique, fatty acid methyl esters (FAMEs) identification in the samples was performed by comparison of the retention times (RT) and the mass/charge (m/z) ratio characteristic of each FAME component in the reference standards used (F.A.M.E. Mix C4 - C24 and SRM®2377). FAMEs quantification from food samples was realized by applying correction factors calculated based on reference standards. NMR spectra were recorded on a Bruker Advance 400 MHz spectrometer, operating at 9.4 Tesla corresponding to the resonance frequency of 400.13 MHz for the 1H nucleus. The NMR spectra was recorded directly on the oil without any sample preparation. The difference between the mean values of the fatty acids content determined by GC-MS and NMR was not more than ± 15% for sunflower oil and lard, and ± 6% for palm oil.
摘要采用气相色谱-质谱联用(GC-MS)和核磁共振(NMR)两种不同的技术,比较了3种食品基质(葵花籽油、棕榈油和猪油)中饱和(SFA)、单不饱和(MUFA)和多不饱和(PUFA)脂肪酸的组成(占总鉴定脂肪酸的重量%)。在GC-MS技术中,通过比较所使用的参考标准品(F.A.M.E. Mix C4 - C24和SRM®2377)中各FAME组分的保留时间(RT)和质量/电荷(m/z)比特征,对样品中的脂肪酸甲基酯(FAMEs)进行鉴定。利用参考标准计算的校正因子,实现了食品样品中各成分的定量。在Bruker Advance 400 MHz谱仪上记录核磁共振谱,工作在9.4特斯拉,对应1H核的共振频率为400.13 MHz。核磁共振光谱直接记录在油上,无需任何样品制备。葵花籽油和猪油的脂肪酸含量平均值和棕榈油的脂肪酸含量平均值相差不超过±15%,棕榈油的脂肪酸含量平均值相差不超过±6%。
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引用次数: 6
Effect of Trifluralin, Zero-Valent Iron and Magnetite Nanoparticles on Growth of Micromycetes 氟乐灵、零价铁和纳米磁铁矿对微霉菌生长的影响
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0085
V. Josan, I. Rastimesina, O. Postolachi, T. Gutul
Abstract Nowadays many reports confirmed the effect of different nanoparticles (NPs) on the growth and secondary metabolite production in various microorganisms. Some of them, NPs like Ag, Au and oxides of Al, Ti, Si and Zn have harmful effect on the cells of microorganisms. Iron NPs are expected to be nontoxic, due to using Fe atom in several pathways of cell metabolism and, therefore, low iron toxicity. The use of iron NPs in technologies for remedying polluted environment was caused by their efficiency in reduction reactions, mobility, and high reactivity, due to the high surface area. The present study aims to determine the effect of magnetite (Fe3O4), zero-valent iron Fe(0) NPs, and fluorinated dinitroaniline herbicide trifluralin on growth of mycelial fungi. Fungal strains were isolated from soil long-term polluted with obsolete pesticides, DDT and trifluralin. The inhibition activity of iron NPs and trifluralin was evaluated using express-method. Each fungal strain had an individual reaction to the solutions of iron nanoparticles. At the same time, Fe(0) NPs, as well as magnetite NPs, had a stimulating effect on the formation and maturation of spores of micromycetes. Addition of trifluralin to the culture medium had a growth inhibition effect on micromycetes, but this effect was reduced, when trifluralin was mixed and incubated with iron NPs for 1 hour before.
近年来,许多报道证实了不同纳米颗粒(NPs)对各种微生物生长和次生代谢物产生的影响。其中一些NPs如Ag、Au和Al、Ti、Si、Zn的氧化物对微生物细胞有有害影响。铁NPs预计是无毒的,因为在细胞代谢的几个途径中使用铁原子,因此具有低铁毒性。铁NPs在污染环境修复技术中的应用主要是由于其高表面积的还原反应效率、迁移率和高反应活性。本研究旨在确定磁铁矿(Fe3O4)、零价铁(Fe (0) NPs)和氟化二硝基苯胺除草剂三氟灵对菌丝真菌生长的影响。从长期被废农药、滴滴涕和氟虫灵污染的土壤中分离到真菌菌株。用表达法对铁NPs和氟乐灵的抑制活性进行评价。每种真菌菌株对铁纳米颗粒溶液都有单独的反应。同时,Fe(0) NPs和磁铁矿NPs对微菌孢子的形成和成熟都有刺激作用。在培养基中加入三氟乐林对微菌有抑制生长的作用,但将三氟乐林与铁NPs混合培养1小时后,这种抑制作用减弱。
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引用次数: 0
Experimental Rearing of the Golden Gray Mullet Liza Aurata (Risso, 1810) in a Recirculating System at the Black Sea 金灰色鲻鱼Liza Aurata (Risso, 1810)在黑海循环系统中的实验饲养
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0022
V. Niță, M. Nenciu, C. Nicolae
Abstract Recirculating Aquaculture Systems (RAS) used in aquatic research and production are a viable alternative to traditional open-sea mariculture. The rearing of marine living resources in controlled environments is one of NIMRD’s constant concerns. Golden gray mullet Liza aurata (Risso, 1810) is spread in the Mediterranean, Black and Caspian Seas, as well as along the Atlantic coast, and culture in extensive and semi-intensive enclosures has been practiced worldwide for centuries, especially in the Far East and the Mediterranean, reaching a global production of 134,329 tons in 2010. The species’ potential for aquaculture is enhanced by its eurihalyne and eurithermal adaptability, allowing it to grow in a variety of ecosystems. The biological material used for the experiment was represented by golden gray mullet fingerlings fished using the beach seine from the Black Sea. They were monitored for health state, growth rythm (mean length and weight determinations), behavior under RAS conditions, survival rate and overall adaptability. The growth of juveniles after 90 days was 89.2% for total length and 246% for mean biomass. Concerning overall adaptability, after approximately one month, the golden gray mullet individuals fully adapted to rearing in the RAS tanks and handling during measurements. Moreover, the food administered (Skretting Classic K) was easily accepted, active feeding behavior being observed even in the presence of NIMRD’s staff. The fish’s health state was good, with no mortalities caused by infections or parasites. The survival rate upon completion of the two monitoring months was 76.66% (23 of the 30 individuals included in the experiment). The preliminary results obtained showed a good adaptability and a normal growth rate, which makes Liza aurata a potential candidate for aquaculture targeting the national and regional market.
循环水养殖系统(RAS)用于水产研究和生产是传统远洋海水养殖的可行替代方案。在受控环境中培育海洋生物资源是NIMRD一直关注的问题之一。金灰色鲻鱼Liza aurata (Risso, 1810)分布在地中海、黑海和里海,以及大西洋沿岸,几个世纪以来,在世界范围内进行了广泛和半密集的圈养,特别是在远东和地中海,2010年全球产量达到134,329吨。该物种在水产养殖方面的潜力因其全盐和全热适应性而增强,使其能够在各种生态系统中生长。实验使用的生物材料是用黑海海滩围网捕捞的金灰色鲻鱼鱼种。监测它们的健康状况、生长节律(平均长度和体重测定)、RAS条件下的行为、存活率和整体适应性。90 d后,幼鱼长高89.2%,平均生物量长高246%。在整体适应性方面,大约一个月后,金灰鲻鱼个体完全适应了RAS水箱中的饲养和测量期间的处理。此外,喂食的食物(Skretting Classic K)很容易被接受,即使在NIMRD的工作人员在场的情况下,也能观察到积极的进食行为。这条鱼的健康状况良好,没有因感染或寄生虫导致死亡。2个监测月后存活率为76.66%(纳入实验的30只中有23只)。初步结果表明,该品种具有良好的适应性和正常的生长速度,是面向全国和区域市场的潜在养殖候选品种。
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引用次数: 5
A Retrospective Radiographic Study Regarding Diffuse Idiopathic Skeletal Hyperostosis (Dish) in Dogs 犬弥漫性特发性骨骼肥厚症(Dish)的回顾性影像学研究
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0077
N. Tudor
Abstract Diffuse idiopathic skeletal hyperostosis (DISH) is a systemic non-inflammatory disease of unknown aetiology affecting the axial and appendicular skeleton. The disease is characterised by calcification of soft tissues including tendons, joint capsules, and ligamentous attachments to bone. A retrospective radiographic study was conducted to investigate the presence and distribution of the DISH in dogs. For this goal were revised medical records from the radiology service of Faculty of Veterinary Medicine Bucharest for dogs that had a radiographic report that described the presence of DISH at spine level. The signalments of all dogs were taken from their medical records. From 531 dogs of 19 breeds, 11 dogs (Cross breed = 3; Rottweiler = 3; Boxer = 2; Golden retriever = 2; German shepherd = 1) were diagnosed with DISH, representing 2.07%. Of these, 5 were females and 6 males, aged between 6 and 15 years (an average of 9.81 years). Following the radiographic evaluation, a total of 74 vertebral segments affected by DISH were identified, and the number of vertebral segments affected per dogs varied between 4 and 10. In all cases, the DISH was accompanied by spondylosis deformans. Although the presence of flowing calcification in the ventral aspects of adjacent vertebral bodies is conclusive for classification as DISH, however it is recommended obtaining supplementation data through modern imaging exams on the vertebral structures.
弥漫性特发性骨骼肥厚症(DISH)是一种病因不明的全身性非炎性疾病,主要影响中轴和尾骨。该疾病的特征是软组织钙化,包括肌腱、关节囊和韧带附着骨。回顾性放射学研究进行了调查的存在和分布的狗DISH。为此,我们对布加勒斯特兽医学院放射科的狗的医疗记录进行了修订,这些狗的放射报告描述了脊柱水平存在DISH。所有狗的信号都取自它们的医疗记录。从19个品种的531只狗中,11只(杂交品种= 3;罗威纳犬= 3;拳击手= 2;金毛= 2;德国牧羊犬1只,占2.07%。其中女性5例,男性6例,年龄6 ~ 15岁(平均9.81岁)。放射学评估后,共鉴定出74个受DISH影响的椎节,每只狗受影响的椎节数在4到10之间。在所有病例中,DISH都伴有脊柱畸形。虽然相邻椎体腹侧出现流动钙化是诊断为DISH的决定性因素,但建议通过对椎体结构的现代影像学检查获得补充数据。
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引用次数: 0
Еpidemiological Data on Lead Tissue Concentration in Game Birds Induced by Lead Pellets Еpidemiological铅丸致猎禽铅组织浓度数据
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0075
P. Stamberov, C. Zhelev, T. Todorov, S. Ivanova, T. Mehmedov, I. Manev, E. Taneva
Abstract The paper presents the level of lead in the liver, gizzard, breast muscles and humerus of game birds, shot during the hunting season of 2016 - 2017 in Bulgaria. In quail gizzards (n=10) radiographic examination showed ingested pellets. In turtle doves (n=10), lead levels in the liver had higher values of 2,501 ± 1,404 mg/kg, compared to the maximum levels of <2 mg/kg. The content of lead in the humerus of partridges (n=10) showed a very high concentrations of 54,241 ± 36,731 mg/kg compared to the base level of 10<20 mg/kg. The high levels of lead in the tissues of the game birds, induced by lead shot exposure, are a significant risk to predators and scavengers.
摘要:本文介绍了2016 - 2017年保加利亚狩猎季节拍摄的猎禽肝脏、砂囊、胸肌和肱骨中的铅水平。在鹌鹑砂囊(n=10)中,x线检查显示摄入颗粒。在斑鸠(n=10)中,肝脏中的铅水平较高,为2,501±1,404 mg/kg,而最高水平为<2 mg/kg。与10<20 mg/kg的基准水平相比,10只鹌鹑肱骨铅含量高达54,241±36,731 mg/kg。由于接触铅弹,猎禽组织中的铅含量很高,这对食肉动物和食腐动物来说是一个重大风险。
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引用次数: 1
The Influence of Nitrogen Fertilization and Fungicides Treatments on Yield and Quality Obtained from Various Premium Wheat Varieties 氮肥和杀菌剂处理对不同优质小麦品种产量和品质的影响
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0007
C. Cioineag, R. Horoias, S. Cristea
Abstract This study analyzes the results obtained during the agricultural year 2015-2016 on the PHD thesis research field and was carried out on a series of 6 wheat varieties (Triticum aestivum L.), of which 5 Premium wheat varieties and one Class A variety. The purpose of this research is to stimulate the expansion of Premium wheat varieties surfaces in Romania. Due to the superior quality of the grains (protein> 14.5%) their use in bakery, will reduce the use of synthesis enhancers. The location of the experimental field was carried out in Draganesti-Vlasca area, at SCDA Teleorman. The aim was to establish optimal technology to maximize the quantity and quality of the yield. Given the crop rotation after peas, the behavior of the wheat varieties was observed on different nitrogen doses. There were applied 3 fertilization fractions with nitrogen (N1-N3) and 3 treatments with fungicides (V1-V3). Determinations of the main foliar and spike diseases were made. The correlation between fertilizer and fungicide treatments showed the differences between yield potential and disease tolerance of the analyzed varieties.
本研究对2015-2016农业年度博士论文研究领域取得的成果进行了分析,以6个小麦品种(Triticum aestivum L.)为研究对象,其中5个为优质小麦品种,1个为a类小麦品种。本研究的目的是促进优质小麦品种在罗马尼亚的推广。由于谷物的优良品质(蛋白质含量为14.5%),它们在烘焙中的使用将减少合成增强剂的使用。试验田的位置在draganestii - vlasca地区,位于SCDA Teleorman。目的是建立最佳工艺,以最大限度地提高产量的数量和质量。在豌豆后轮作的条件下,观察了不同施氮量对小麦品种的影响。施氮3个处理(n1 ~ n3),杀真菌剂3个处理(v1 ~ v3)。对主要叶面和穗部病害进行了测定。肥料和杀菌剂处理的相关性表明,所分析品种的产量潜力和抗病性存在差异。
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引用次数: 0
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