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Research on the Technological and Morpho-Anatomic Particularities on Forcing Bulbs of Iris Reticulata 网纹鸢尾催芽技术及形态解剖特点的研究
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0048
F. Toma, M. Georgescu, S. Petra, C. Mãnescu, E. Ailincai, D. C. Potor
Abstract Iris reticulata is one of the most appreciated flower species, being found in both field crops and greenhouse cultures. Our research has as a major objective the study of the influence of particularities of thermic regime from cold period upon the quality of the bulbs flowering. For planting we used bulbs distributed in three equal batches, for each batch, differentiating the period of cold necessary for the rooting of the bulbs. The cold period was provided in three variants: in the refrigerator (5°C), in a shade which climate similar to the outside (5°C), in the cellar (10°C). The introduction of bulbs planted in the greenhouse for forcing was performed for each of the three batches after 4, 6 and 8 weeks of cold storage. The microscopic preparations show that on begin of cold period all the floral elements are formed inside in the bulb. The best quality of the bulbs flowering was obtained on the variants which cold period occurred on the shade, at °5 C temperature.
网纹鸢尾(Iris reticulata)是一种最受欢迎的花卉,在大田作物和温室栽培中都有发现。本研究的主要目的是研究冷期热状态的特殊性对球茎开花质量的影响。种植时,我们将球茎均匀地分成三批,每批区分球茎生根所需的低温期。冷期有三种形式:在冰箱里(5°C),在与外界气候相似的阴凉处(5°C),在地窖里(10°C)。在冷藏4周、6周和8周后,三个批次的球茎都被引入温室进行强制种植。显微观察表明,在寒冷期开始时,所有的花元素都在球茎内部形成。在阴凉处发生冷期、温度为°5℃的变异球茎开花质量最好。
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引用次数: 0
The Romanian Agrotourism Over the Last Two Decades: Good Old Places or New Attractions? 过去二十年的罗马尼亚农业旅游:好地方还是新景点?
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0027
Beatris Tenie, G. Fîntîneru, D. Smedescu, A. Fîntîneru
Abstract Tourism represents an important economic activity for most of the countries all around the world. For Romania also, tourism plays an extremely important role in the economic development, contributing with 5.2% to GDP and with more than 500,000 for total employment, according to the 2017 WTTC report. Starting with the year 2000, the Romanian rural tourism and agrotourism, have seen a significant development in terms of infrastructure and services quality, political attention, legislative climate and qualified staff. In order to highlight the dynamics of Romanian agrotourism and to deliver insights about the greatest challenges of this activity in rural areas, the paper investigated the main offer and demand regional indicators of agro-tourist activity in Romania during the period 2000-2016, using the empirical data provided by the National Institute of Statistics.
对世界上大多数国家来说,旅游业是一项重要的经济活动。根据2017年WTTC的报告,旅游业在罗马尼亚的经济发展中也发挥着极其重要的作用,对GDP的贡献为5.2%,总就业人数超过50万人。从2000年开始,罗马尼亚农村旅游和农业旅游在基础设施和服务质量、政治注意、立法气氛和合格工作人员方面取得了重大发展。为了突出罗马尼亚农业旅游的动态,并提供有关该活动在农村地区面临的最大挑战的见解,本文使用国家统计局提供的经验数据,调查了2000-2016年期间罗马尼亚农业旅游活动的主要提供和需求区域指标。
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引用次数: 1
Optimizing the Operation of Fermenters 优化发酵罐的运行
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0056
D. Drăcea, A. Tronac, Sebastian Mustata
Abstract World energy systems development is based on the use of renewable sources, their valorisation benefits of technical and economical stimulating actions. The main disadvantage is related to the low efficiency caused by low energy density and high value investments. The cost of renewable energy is higher than energy coming from conventional sources. Reducing the costs of producing unconventional energy can be achieved by integrating economic and social activities into the system, providing futher benefits. From the potential point of view, it is estimated that biomass occupies the first place in a ranking of unconventional energies. Biomass boosted by anaerobic fermentation with biogas production and cogeneration can provide "peak" energy production and can balance energy systems. Waste utilized by these processes involves reducing environmental impacts by diminushing managed volumes and efficient control. In the present paper are characterized by calculations aspects of the exploitation and elements regarding the optimization of some high-capacity fermenters of a wastewater treatment system.
世界能源系统的发展是基于可再生能源的使用,其增值效益的技术和经济刺激行动。主要的缺点是低能量密度和高价值投资导致的低效率。可再生能源的成本高于传统能源。通过将经济和社会活动整合到系统中,可以降低非常规能源的生产成本,从而带来进一步的效益。从潜力的角度来看,估计生物质能在非常规能源的排名中占据首位。由厌氧发酵与沼气生产和热电联产推动的生物质可以提供“峰值”能源生产,并可以平衡能源系统。这些过程利用的废物涉及通过减少管理的数量和有效控制来减少对环境的影响。本文的特点是计算方面的开发和要素有关的一些大容量发酵罐的废水处理系统的优化。
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引用次数: 0
Quantifying Bisphenol from Food Packaging and Assessment of its Cytotoxic Potential 食品包装中双酚的定量测定及其细胞毒性评估
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0078
Elena Ungureanu, G. Mustățea, Loredana Stanca, Iren Şerban
Abstract Bisphenol A (BPA) is a compound that is released from PET packaging during storage bottled water and can have a harmful effect on human health. This study aimed to investigate the cytotoxic potential of BPA at the Caco-2 cell line. Bottled water samples coming from two companies were analyzed starting from 1 month to 12 months of storage Quantification of BPA in water stored in PET containers was carried out by a spectrometric method. Calibration was performed for BPA concentrations ranging between 0.01 and 0.09 mg/L. Caco-2 cell cultures were treated for 24 hours with different BPA concentrations (from 0.1 mM to 1 mM). Viability and cell cytotoxicity analysis was performed by MTT assay. BPA concentration in one water sample ranged from 0.09 to 0.92 mg/L while for second water sample BPA concentration ranged from 0.03 to 0.73 mg/L. After 10 months from packing, BPA concentration began to exceed the 0.6 mg/L limit stated by Regulation (EU) no. 10/2011. Cell viability decreased in a dose dependent manner and reached the minimum of 3.8% for the 1 mM BPA concentration.
摘要双酚A (BPA)是瓶装水在储存过程中从PET包装中释放出来的一种对人体健康有害的化合物。本研究旨在探讨BPA对Caco-2细胞系的细胞毒性。来自两家公司的瓶装水样品从储存1个月到12个月开始进行分析,用光谱法对储存在PET容器中的水中的BPA进行定量分析。校正BPA浓度范围为0.01 ~ 0.09 mg/L。不同BPA浓度(0.1 mM ~ 1mm)处理Caco-2细胞培养24小时。MTT法测定细胞活力和细胞毒性。一个水样的BPA浓度范围为0.09 ~ 0.92 mg/L,另一个水样的BPA浓度范围为0.03 ~ 0.73 mg/L。包装10个月后,BPA浓度开始超过法规(EU) no. 6规定的0.6 mg/L限值。10/2011。细胞活力呈剂量依赖性下降,当BPA浓度为1 mM时,细胞活力最低为3.8%。
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引用次数: 1
Observations on Xylazine-Ketamine-Isoflurane Anesthesia in Constrictor Snakes 噻嗪-氯胺酮-异氟醚麻醉对巨蟒的疗效观察
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0074
L. Schuszler, D. Popović, C. Zaha, B. Sicoe, D. Bumb, C. Igna
Abstract Anesthetic management of the reptilian patient, including the design of the anesthetic protocol, is a challenge due to multiple anatomical and physiological features. Usually, anesthetic and analgesia protocols, including monitoring techniques, some of which are of low value, established in domestic animals are also applied to these patients. In the study were included five constrictor snakes in which, under xylazine-ketamine-isoflurane anesthesia, various surgical interventions lasting up to 50 minutes were performed. The severity of CNS inhibition as well as the length of different anesthetic times was monitored. The association used ensured the achievement of the surgical anesthesia depth and immobility with optimal conditions for performing surgical interventions in the oral cavity, cutaneous or amputation of hemipenis. The length of induction, surgical anesthesia and recovery fall within the limits recorded in the specialty literature for reptile.
爬行动物患者的麻醉管理,包括麻醉方案的设计,由于多种解剖和生理特征,是一个挑战。通常,麻醉和镇痛方案,包括监测技术,其中一些是低价值的,建立在家畜也适用于这些患者。在这项研究中包括五条蟒蛇,在二甲肼-氯胺酮-异氟醚麻醉下,进行了持续50分钟的各种手术干预。观察不同麻醉时间对中枢神经系统抑制程度的影响。该关联确保了在口腔、皮肤或半阴茎截肢手术中实现手术麻醉深度和固定化的最佳条件。诱导、手术麻醉和恢复的时间长度在爬行动物专业文献中记录的范围内。
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引用次数: 0
Effect of Trifluralin, Zero-Valent Iron and Magnetite Nanoparticles on Growth of Micromycetes 氟乐灵、零价铁和纳米磁铁矿对微霉菌生长的影响
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0085
V. Josan, I. Rastimesina, O. Postolachi, T. Gutul
Abstract Nowadays many reports confirmed the effect of different nanoparticles (NPs) on the growth and secondary metabolite production in various microorganisms. Some of them, NPs like Ag, Au and oxides of Al, Ti, Si and Zn have harmful effect on the cells of microorganisms. Iron NPs are expected to be nontoxic, due to using Fe atom in several pathways of cell metabolism and, therefore, low iron toxicity. The use of iron NPs in technologies for remedying polluted environment was caused by their efficiency in reduction reactions, mobility, and high reactivity, due to the high surface area. The present study aims to determine the effect of magnetite (Fe3O4), zero-valent iron Fe(0) NPs, and fluorinated dinitroaniline herbicide trifluralin on growth of mycelial fungi. Fungal strains were isolated from soil long-term polluted with obsolete pesticides, DDT and trifluralin. The inhibition activity of iron NPs and trifluralin was evaluated using express-method. Each fungal strain had an individual reaction to the solutions of iron nanoparticles. At the same time, Fe(0) NPs, as well as magnetite NPs, had a stimulating effect on the formation and maturation of spores of micromycetes. Addition of trifluralin to the culture medium had a growth inhibition effect on micromycetes, but this effect was reduced, when trifluralin was mixed and incubated with iron NPs for 1 hour before.
近年来,许多报道证实了不同纳米颗粒(NPs)对各种微生物生长和次生代谢物产生的影响。其中一些NPs如Ag、Au和Al、Ti、Si、Zn的氧化物对微生物细胞有有害影响。铁NPs预计是无毒的,因为在细胞代谢的几个途径中使用铁原子,因此具有低铁毒性。铁NPs在污染环境修复技术中的应用主要是由于其高表面积的还原反应效率、迁移率和高反应活性。本研究旨在确定磁铁矿(Fe3O4)、零价铁(Fe (0) NPs)和氟化二硝基苯胺除草剂三氟灵对菌丝真菌生长的影响。从长期被废农药、滴滴涕和氟虫灵污染的土壤中分离到真菌菌株。用表达法对铁NPs和氟乐灵的抑制活性进行评价。每种真菌菌株对铁纳米颗粒溶液都有单独的反应。同时,Fe(0) NPs和磁铁矿NPs对微菌孢子的形成和成熟都有刺激作用。在培养基中加入三氟乐林对微菌有抑制生长的作用,但将三氟乐林与铁NPs混合培养1小时后,这种抑制作用减弱。
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引用次数: 0
Experimental Rearing of the Golden Gray Mullet Liza Aurata (Risso, 1810) in a Recirculating System at the Black Sea 金灰色鲻鱼Liza Aurata (Risso, 1810)在黑海循环系统中的实验饲养
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0022
V. Niță, M. Nenciu, C. Nicolae
Abstract Recirculating Aquaculture Systems (RAS) used in aquatic research and production are a viable alternative to traditional open-sea mariculture. The rearing of marine living resources in controlled environments is one of NIMRD’s constant concerns. Golden gray mullet Liza aurata (Risso, 1810) is spread in the Mediterranean, Black and Caspian Seas, as well as along the Atlantic coast, and culture in extensive and semi-intensive enclosures has been practiced worldwide for centuries, especially in the Far East and the Mediterranean, reaching a global production of 134,329 tons in 2010. The species’ potential for aquaculture is enhanced by its eurihalyne and eurithermal adaptability, allowing it to grow in a variety of ecosystems. The biological material used for the experiment was represented by golden gray mullet fingerlings fished using the beach seine from the Black Sea. They were monitored for health state, growth rythm (mean length and weight determinations), behavior under RAS conditions, survival rate and overall adaptability. The growth of juveniles after 90 days was 89.2% for total length and 246% for mean biomass. Concerning overall adaptability, after approximately one month, the golden gray mullet individuals fully adapted to rearing in the RAS tanks and handling during measurements. Moreover, the food administered (Skretting Classic K) was easily accepted, active feeding behavior being observed even in the presence of NIMRD’s staff. The fish’s health state was good, with no mortalities caused by infections or parasites. The survival rate upon completion of the two monitoring months was 76.66% (23 of the 30 individuals included in the experiment). The preliminary results obtained showed a good adaptability and a normal growth rate, which makes Liza aurata a potential candidate for aquaculture targeting the national and regional market.
循环水养殖系统(RAS)用于水产研究和生产是传统远洋海水养殖的可行替代方案。在受控环境中培育海洋生物资源是NIMRD一直关注的问题之一。金灰色鲻鱼Liza aurata (Risso, 1810)分布在地中海、黑海和里海,以及大西洋沿岸,几个世纪以来,在世界范围内进行了广泛和半密集的圈养,特别是在远东和地中海,2010年全球产量达到134,329吨。该物种在水产养殖方面的潜力因其全盐和全热适应性而增强,使其能够在各种生态系统中生长。实验使用的生物材料是用黑海海滩围网捕捞的金灰色鲻鱼鱼种。监测它们的健康状况、生长节律(平均长度和体重测定)、RAS条件下的行为、存活率和整体适应性。90 d后,幼鱼长高89.2%,平均生物量长高246%。在整体适应性方面,大约一个月后,金灰鲻鱼个体完全适应了RAS水箱中的饲养和测量期间的处理。此外,喂食的食物(Skretting Classic K)很容易被接受,即使在NIMRD的工作人员在场的情况下,也能观察到积极的进食行为。这条鱼的健康状况良好,没有因感染或寄生虫导致死亡。2个监测月后存活率为76.66%(纳入实验的30只中有23只)。初步结果表明,该品种具有良好的适应性和正常的生长速度,是面向全国和区域市场的潜在养殖候选品种。
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引用次数: 5
A Retrospective Radiographic Study Regarding Diffuse Idiopathic Skeletal Hyperostosis (Dish) in Dogs 犬弥漫性特发性骨骼肥厚症(Dish)的回顾性影像学研究
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0077
N. Tudor
Abstract Diffuse idiopathic skeletal hyperostosis (DISH) is a systemic non-inflammatory disease of unknown aetiology affecting the axial and appendicular skeleton. The disease is characterised by calcification of soft tissues including tendons, joint capsules, and ligamentous attachments to bone. A retrospective radiographic study was conducted to investigate the presence and distribution of the DISH in dogs. For this goal were revised medical records from the radiology service of Faculty of Veterinary Medicine Bucharest for dogs that had a radiographic report that described the presence of DISH at spine level. The signalments of all dogs were taken from their medical records. From 531 dogs of 19 breeds, 11 dogs (Cross breed = 3; Rottweiler = 3; Boxer = 2; Golden retriever = 2; German shepherd = 1) were diagnosed with DISH, representing 2.07%. Of these, 5 were females and 6 males, aged between 6 and 15 years (an average of 9.81 years). Following the radiographic evaluation, a total of 74 vertebral segments affected by DISH were identified, and the number of vertebral segments affected per dogs varied between 4 and 10. In all cases, the DISH was accompanied by spondylosis deformans. Although the presence of flowing calcification in the ventral aspects of adjacent vertebral bodies is conclusive for classification as DISH, however it is recommended obtaining supplementation data through modern imaging exams on the vertebral structures.
弥漫性特发性骨骼肥厚症(DISH)是一种病因不明的全身性非炎性疾病,主要影响中轴和尾骨。该疾病的特征是软组织钙化,包括肌腱、关节囊和韧带附着骨。回顾性放射学研究进行了调查的存在和分布的狗DISH。为此,我们对布加勒斯特兽医学院放射科的狗的医疗记录进行了修订,这些狗的放射报告描述了脊柱水平存在DISH。所有狗的信号都取自它们的医疗记录。从19个品种的531只狗中,11只(杂交品种= 3;罗威纳犬= 3;拳击手= 2;金毛= 2;德国牧羊犬1只,占2.07%。其中女性5例,男性6例,年龄6 ~ 15岁(平均9.81岁)。放射学评估后,共鉴定出74个受DISH影响的椎节,每只狗受影响的椎节数在4到10之间。在所有病例中,DISH都伴有脊柱畸形。虽然相邻椎体腹侧出现流动钙化是诊断为DISH的决定性因素,但建议通过对椎体结构的现代影像学检查获得补充数据。
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引用次数: 0
Еpidemiological Data on Lead Tissue Concentration in Game Birds Induced by Lead Pellets Еpidemiological铅丸致猎禽铅组织浓度数据
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0075
P. Stamberov, C. Zhelev, T. Todorov, S. Ivanova, T. Mehmedov, I. Manev, E. Taneva
Abstract The paper presents the level of lead in the liver, gizzard, breast muscles and humerus of game birds, shot during the hunting season of 2016 - 2017 in Bulgaria. In quail gizzards (n=10) radiographic examination showed ingested pellets. In turtle doves (n=10), lead levels in the liver had higher values of 2,501 ± 1,404 mg/kg, compared to the maximum levels of <2 mg/kg. The content of lead in the humerus of partridges (n=10) showed a very high concentrations of 54,241 ± 36,731 mg/kg compared to the base level of 10<20 mg/kg. The high levels of lead in the tissues of the game birds, induced by lead shot exposure, are a significant risk to predators and scavengers.
摘要:本文介绍了2016 - 2017年保加利亚狩猎季节拍摄的猎禽肝脏、砂囊、胸肌和肱骨中的铅水平。在鹌鹑砂囊(n=10)中,x线检查显示摄入颗粒。在斑鸠(n=10)中,肝脏中的铅水平较高,为2,501±1,404 mg/kg,而最高水平为<2 mg/kg。与10<20 mg/kg的基准水平相比,10只鹌鹑肱骨铅含量高达54,241±36,731 mg/kg。由于接触铅弹,猎禽组织中的铅含量很高,这对食肉动物和食腐动物来说是一个重大风险。
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引用次数: 1
The Influence of Nitrogen Fertilization and Fungicides Treatments on Yield and Quality Obtained from Various Premium Wheat Varieties 氮肥和杀菌剂处理对不同优质小麦品种产量和品质的影响
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0007
C. Cioineag, R. Horoias, S. Cristea
Abstract This study analyzes the results obtained during the agricultural year 2015-2016 on the PHD thesis research field and was carried out on a series of 6 wheat varieties (Triticum aestivum L.), of which 5 Premium wheat varieties and one Class A variety. The purpose of this research is to stimulate the expansion of Premium wheat varieties surfaces in Romania. Due to the superior quality of the grains (protein> 14.5%) their use in bakery, will reduce the use of synthesis enhancers. The location of the experimental field was carried out in Draganesti-Vlasca area, at SCDA Teleorman. The aim was to establish optimal technology to maximize the quantity and quality of the yield. Given the crop rotation after peas, the behavior of the wheat varieties was observed on different nitrogen doses. There were applied 3 fertilization fractions with nitrogen (N1-N3) and 3 treatments with fungicides (V1-V3). Determinations of the main foliar and spike diseases were made. The correlation between fertilizer and fungicide treatments showed the differences between yield potential and disease tolerance of the analyzed varieties.
本研究对2015-2016农业年度博士论文研究领域取得的成果进行了分析,以6个小麦品种(Triticum aestivum L.)为研究对象,其中5个为优质小麦品种,1个为a类小麦品种。本研究的目的是促进优质小麦品种在罗马尼亚的推广。由于谷物的优良品质(蛋白质含量为14.5%),它们在烘焙中的使用将减少合成增强剂的使用。试验田的位置在draganestii - vlasca地区,位于SCDA Teleorman。目的是建立最佳工艺,以最大限度地提高产量的数量和质量。在豌豆后轮作的条件下,观察了不同施氮量对小麦品种的影响。施氮3个处理(n1 ~ n3),杀真菌剂3个处理(v1 ~ v3)。对主要叶面和穗部病害进行了测定。肥料和杀菌剂处理的相关性表明,所分析品种的产量潜力和抗病性存在差异。
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引用次数: 0
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