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The Influence of Climatic Variations on the Stability of Wheat Plant Height 气候变化对小麦株高稳定性的影响
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0080
S. Barbu, A. Giura, Daniele Cristina, C. Cornea
Abstract The decrease of variability sources and the genotypes instability as a result of climate changes from the last years were issues addressed in the breeding programs. For breeders the stability of some parameters like plant height is very important in selection process, therefore the influence of climatic variations it is aimed to be reduced by genetic response of the genotypes. Semi-dwarf genotypes are preferred by breeders because in this way is avoided the lodging risk. A set of 307 mutant and mutant/recombinant wheat DH lines, along with parental genotypes, was analyzed in three different years for plant height and the semi-dwarf selected lines were evaluated for stability of this parameter. The material was also analyzed for the presence of Lr34, or genes and 1A/1R translocation. In our study the reduced plant height and its stability was not associated with any of the genetic background
近年来气候变化导致变异源的减少和基因型的不稳定性是育种计划中需要解决的问题。对于育种者来说,株高等参数的稳定性在选择过程中是非常重要的,因此旨在通过基因型的遗传响应来降低气候变化的影响。半矮秆基因型是育种者的首选,因为这样可以避免倒伏风险。对307个突变和突变/重组小麦DH系及其亲本基因型在3个不同年份的株高进行了分析,并对半矮秆选育系的株高稳定性进行了评价。还分析了材料中是否存在Lr34或基因和1A/1R易位。在我们的研究中,株高的降低及其稳定性与任何遗传背景无关
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引用次数: 1
Clinical and Epidemiological Study on Aelurostrongylus Abstrusus Infection in Cats, Romania; Preliminary Data 罗马尼亚猫隐杆线虫感染的临床及流行病学研究初步的数据
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0064
R. Ciopașiu, Elena Florina Berbece, M. Ioniță, I. Mitrea
Abstract The lungworm Aelurostrongylus abstrusus (Nematoda: Angiostrongylidae) is a parasite of domestic and wild felids with worldwide distribution. The parasite lives in bronchioles and alveolar ducts, and its presence is associated with respiratory clinical signs. Cat lungworms are still considered by many clinicians sporadic and rarely are taken into account in the cat pathology. Therefore, in this paper we present a clinical and epidemiological study on cats with natural A. abstrusus infection. For this, 131 cats, with exclusive outdoor or outdoor and indoor access, of different gender (49 males, 82 females) and age (between 2 months and 11 years), from Southern and Center Romania, were included in the study. Of them, individual fresh faecal samples were collected and analyzed to identify first-stage larvae (L1) of lungworms, using a Baermann technique, and eggs/oocysts of other endoparasites, by a flotation method. Subsequently, of the examined samples, 6.10% (8/131) were positive for A. abstrusus. The positive cats aged between 3 months and 2 years. Of them, 3 cats were co-infected: two with Toxocara cati and one with Ancylostoma tubaeforme. Clinically, 75% (6/8) of the infected cats displayed respiratory signs varying from mild (sporadic coughing, sneezing) (n=3) to severe symptoms (mucopurulent nasal discharge, abdominal breathing) (n=3), while 25% (2/8) showed no respiratory signs. The three cats with severe respiratory disease were infected only with A.abstrusus. In conclusion, veterinary practitioners should include Aelurostrongylus infection in the differential diagnosis of catʼs respiratory pathology.
摘要/ Abstract摘要:细孔线虫(线虫纲:管圆线虫科)是一种广泛分布于家养和野生猫科动物的寄生虫。这种寄生虫生活在细支气管和肺泡管中,它的存在与呼吸临床症状有关。许多临床医生仍然认为猫肺虫是散发性的,很少被考虑到猫的病理。因此,本文对猫天然感染的临床和流行病学进行了研究。为此,研究人员将来自罗马尼亚南部和中部的131只猫纳入研究范围,这些猫的性别(49只雄性,82只雌性)和年龄(2个月至11岁)不同,只能在室外或室外和室内活动。收集新鲜粪便样本,采用Baermann法鉴定肺虫的第一阶段幼虫(L1),采用浮选法鉴定其他内寄生虫的卵/卵囊。随后,检出的样品中,有6.10%(8/131)呈阳性。阳性猫的年龄在3个月至2岁之间。其中合并感染猫弓形虫2只,管状钩虫1只。临床上,75%(6/8)的感染猫表现出呼吸体征,症状从轻度(零星咳嗽、打喷嚏)(n=3)到重度(粘液脓性鼻分泌物、腹式呼吸)(n=3)不等,25%(2/8)的感染猫无呼吸体征。3只患严重呼吸道疾病的猫只感染了抽象单胞菌。总之,兽医从业人员应在猫呼吸道病理鉴别诊断中纳入丝轮线虫感染。
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引用次数: 1
Control of Coffee Leaf Rust by Chitosan Oligomers and Propolis 壳聚糖低聚物和蜂胶防治咖啡叶锈病
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0046
Iosody Silva-Castro, R. Barreto, M. C. Rodriguez, P. M. Matei, J. Martín‐Gil
Abstract Broad scale use of chemical pesticides is known to have produced broad scale environmental impact worldwide and, at the same time, discovery and registration of new molecules to be used as insecticides, herbicides and fungicides have slowed significantly along the last decades, reducing the number of options for pest control by the farmers. Searches for novel environmentally friendly products are a recognized priority. In this work, aqueous solutions of chitosan oligomers (COs) and propolis (P), individually or as binary mixtures, were tested against Hemileia vastatrix, the fungus which causes coffee leaf rust, the worst disease of coffee. Germination of H. vastatrix was inhibited up toll 99 % by COs, followed by the mixture of COs-P and P individually (germination inhibition of 96 % and 54 % respectively). Testing those products on detached coffee leaf discs also produced promising results when COs were applied before H. vastatrix inoculation. These results may lead to the discovery of new antifungal products for the control of coffee leaf rust.
众所周知,化学农药的大规模使用在世界范围内产生了广泛的环境影响,同时,在过去的几十年里,用于杀虫剂、除草剂和杀菌剂的新分子的发现和登记显著放缓,减少了农民控制害虫的选择数量。寻找新的环保产品是公认的优先事项。在这项工作中,壳聚糖低聚物(COs)和蜂胶(P)的水溶液分别或作为二元混合物,对引起咖啡叶锈病(咖啡最严重的疾病)的真菌半孢菌进行了试验。COs-P和P混合处理对野田葵萌发的抑制作用最高达99%,其次为COs-P和P单独处理对野田葵萌发的抑制作用分别为96%和54%。在分离的咖啡叶盘上测试这些产品时,在接种天花之前施用COs也产生了有希望的结果。这些结果可能导致新的抗真菌产品的发现,以控制咖啡叶锈病。
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引用次数: 7
Influence of Different Storage Methods on Apples Chemical Proprieties 不同贮藏方法对苹果化学特性的影响
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0047
D. Sumedrea, A. Florea, M. Sumedrea, A. Asănică, R. Coman, M. Militaru, E. Chițu, M. Butac, F. Marin, M. Călinescu
Abstract The objective of preservation is to keep fruit fresh as long as possible after harvesting, without major physical, chemical or biological changes in their composition. The experimental factors underlying it the organization scheme are: A Factor - apple varieties: ‘Idared’, ‘Goldrush’, ‘Florina’, ‘Pinova’, ‘Dalinette’, ‘Golden reinderes‘,‘Golden lassa‘,‘Ariane‘; B factor - storage methods, with three graduations: classical method - low temperature and high humidity (1-4ºC; humidity 85-90%), Janny MT box storage method (1-4 ºC; 95-100% humidity; O2 1-3%; CO2 2-5%), fruit control equipment box-pallets (1- 4ºC; 90-95% humidity; O2 1-3%; CO2 2-5%) and factor C - fruit storage period -at 3, 4 and 5 months after harvest respectively. On the average of the cultivars taken in the study, on observe the tendency to increase the total dry mater and total sugar content, and decrease the total tritrable acidity and vitamin C with the prolongation of the fruit storage period.
保存的目的是在收获后尽可能长时间地保持水果的新鲜,使其成分不发生重大的物理、化学或生物变化。组织方案背后的实验因素是:A因素-苹果品种:' Idared ', ' Goldrush ', ' Florina ', ' Pinova ', ' Dalinette ', ' Golden reinderes ', ' Golden lassa ', ' Ariane ';B因素-储存方法,有三个等级:经典方法-低温高湿(1-4ºC);湿度85-90%),Janny MT箱储存法(1-4℃;95 - 100%的湿度;O2 1 - 3%;二氧化碳2-5%),水果控制设备箱托盘(1- 4ºC;90 - 95%的湿度;O2 1 - 3%;CO2 2-5%)和因子C -果实贮藏期-分别在收获后3、4和5个月。平均来看,随着果实贮藏期的延长,各品种的总干物质和总糖含量呈上升趋势,总酸度和维生素C含量呈下降趋势。
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引用次数: 5
The Effect of Different Dietary Calcium Levels on Calcium Metabolism in Laying Hens 饲粮不同钙水平对蛋鸡钙代谢的影响
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0070
I. Negoiță, F. Bărbuceanu, N. Dojană, R. Bălăceanu
Abstract The experiment was carried out on four groups of Hy-Line var. Brown hens (n = 350 each) fed on diets which provided 2.49, 3.07, 3.78 and, respectively, 4.15 g% calcium (Ca) during the laying period. Consequently, mean daily Ca intake was 2.74, 3.38, 4.16 and 4.60 g/cap., respectively. Ca metabolism was assayed at 20 weeks of age (start of the laying), 36 weeks of age (the peak of the laying) and 68 weeks (the end of the laying period). A significant part of the ingested Ca was not absorbed. The percentage of Ca exonerated by feces was higher at the start of the laying and lowers in the peak of laying and at the end of the laying period. The amount of Ca exonerated as unabsorbed Ca increases as the amount of ingested Ca increases inside of each age level. It was found also an increase of the Ca content of the egg shell according the Ca intake statistically significant (P<0.05) in 36- and 68-wk.-old hens, but not in 20-wk.-old hens. Blood plasma Ca showed significantly higher values in 3.38-, 4.16- and 4.60-g/day-Ca-intake groups when compared by 2.74-g/day-Ca-intake group (P < 0.05) but the level of increasing showed lowest values in hen groups with highest intake of Ca. The daily eggshell Ca export strongly correlates with the amount of daily Ca intake in 36-wk.-old hens and 68-wk.-old hens: r = +0.72 and +0.84, respectively, but no correlation was found in 20-wk.-old hens (r = -0.74, negative correlation). The results showed a limited physiologic capacity of the Ca absorption in hen, the Ca surplus being lost. Dietary Ca level should be closely correlated with the laying level of hens.
摘要本试验选用4组海兰品种褐蛋鸡(n = 350),在产蛋期分别饲喂钙含量为2.49、3.07、3.78和4.15 g的饲粮。平均日钙摄入量分别为2.74、3.38、4.16和4.60 g/头。,分别。分别在20周龄(产蛋初期)、36周龄(产蛋高峰期)和68周龄(产蛋末期)测定钙代谢。摄入的钙有很大一部分没有被吸收。粪钙排出率在产蛋初期较高,产蛋高峰期和产蛋末期较低。作为未吸收的钙的量随着每个年龄段内摄入钙的量的增加而增加。在第36周和第68周,蛋壳钙含量随钙摄入量的增加而增加,差异有统计学意义(P<0.05)。-老母鸡,但不是在20周内。孩子只母鸡。3.38、4.16和4.60 g/d钙摄取量组血浆钙含量显著高于2.74 g/d钙摄取量组(P < 0.05),但钙摄取量最高的组血浆钙含量增幅最低。36周蛋壳日钙排泄量与日钙摄取量呈极显著相关。-老母鸡和68周。-龄母鸡:r分别= +0.72和+0.84,但20周无相关性。-老母鸡(r = -0.74,负相关)。结果表明,母鸡对钙的生理吸收能力有限,多余的钙逐渐流失。饲粮钙水平应与蛋鸡产蛋水平密切相关。
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引用次数: 0
Canine Amniotic Membrane Derived Mesenchymal Stem Cells- Potential Sources for Regenerative Medicine 犬羊膜间充质干细胞-再生医学的潜在来源
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0071
E. Páll, R. Pop, S. Ciupe, M. Cenariu, I. Groza
Abstract Canine mesenchymal stem cells (MSCs) can be defined with self renew potential and specific differentiation capacity. Amiotic membrane represent an important source of MSCs, which can be harvested by minimally invasive methods. The aim of our study was to evaluate the growth characteristics of canine amniotic membrane derived mesenchymal stem cells. The placenta samples were collected after cesarean section from healthy mixed breed dogs. MSCs isolation was performed using enzymatic method. Isolated cells were cultured in propagation medium: Dulbecco’s Modified Eagle’s Medium/F12 (DMEM/F12, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% antibiotic-antimycotic (Sigma-Aldrich). The medium was changed after 4 days. The cell doubling number, cell proliferation capacity, cell doubling time, daily duplication rate and clonogenic efficacy were evaluated. Our study demonstrate the self renew potential of canine amniotic membrane derived mesenchymal stem cells, and can represent a potential source of stem cells for canine regenerative medicine.
犬间充质干细胞(MSCs)具有自我更新潜能和特异性分化能力。Amiotic膜是MSCs的重要来源,可以通过微创方法获得。本研究的目的是评价犬羊膜间充质干细胞的生长特性。选取健康杂交犬剖宫产后的胎盘标本。采用酶法分离MSCs。分离细胞在培养基中培养:Dulbecco 's Modified Eagle 's medium /F12 (DMEM/F12, Gibco)中添加10%胎牛血清(FBS, Gibco)和1%抗生素-抗真菌药(Sigma-Aldrich)。4天后更换培养基。观察细胞倍增数、细胞增殖能力、细胞倍增时间、日复制率和克隆效果。我们的研究证明了犬羊膜间充质干细胞的自我更新潜力,可以代表犬再生医学干细胞的潜在来源。
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引用次数: 1
First Outcomes of Grafting Wolfberry in Romania 罗马尼亚枸杞嫁接的首批成果
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0037
A. Asănică, D. Hoza, A. Iacob
Abstract Grafting berries is a topic untouched by researchers or practitioners till now in Romania due to the convenient and widely spread way of classic propagation methods. Nevertheless, the positive effect of grafted plants in commercial orchards is well known at many other species. Traits like precocity, uniformity, fruit size and yield stability is desired to be achieved in this manner. By grafting berries, we look forward to eliminate the actual shortcomings of the traditional crop systems, mainly generated by bushy crown shape that requires large distance between the plants, difficulties in mowing or weeding process along the rows, pruning and harvest operation in a uncomfortable arch position, discontinuous application of pesticides inside the bush etc. In this regard, different methods and trials have been conducted in the last two years at the University of Agronomic Sciences and Veterinary Medicine Bucharest in order to eliminate these bottlenecks in the berry technology trough grafted plants.
摘要:在罗马尼亚,由于经典的浆果嫁接方法方便且广泛传播,至今仍是研究人员或从业者未触及的话题。然而,嫁接植物在商业果园中的积极作用在许多其他物种中是众所周知的。早熟、均匀性、果实大小和产量稳定性等性状是通过这种方式获得的。通过嫁接浆果,我们希望消除传统作物系统的实际缺点,主要是由于灌木冠状,植株之间需要较大的距离,沿行修剪或除草困难,修剪和收获在一个不舒服的弓形位置,在灌木内不连续地施用农药等。在这方面,过去两年在布加勒斯特农艺科学和兽医大学进行了不同的方法和试验,以便通过嫁接植物消除浆果技术中的这些瓶颈。
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引用次数: 1
MRI Findings in Choroid Plexus Carcinoma in Two Dogs 两只犬脉络膜丛癌的MRI表现
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0073
Mihai Săvescu, A. Neagu, C. Vlăgioiu, N. Tudor, T. Soare, I. Raus, G. Predoi
Abstract Due to technological advances in bio-imaging in the last years, magnetic resonance imaging (MRI) is widely used as a noninvasive and non-irradiant tool for assessment and imaging diagnosis. In this study are presented two dogs with progressive clinical and neurological manifestations, following the discovered signs (head tilt and circling in the first case, respective epileptic seizures and ataxia in the second case), MRI evaluation was recommended. The imaging exams showed the presence of a mass, located in the third ventricle, with heterogeneous signal changes in conventional sequences T1, T2 and FLAIR. Unifying the imaging data with those of the histopathological examination it was possible to establish the diagnosis of choroid plexus carcinoma in both cases.
摘要近年来,由于生物成像技术的进步,磁共振成像(MRI)作为一种无创、无辐射的评估和成像诊断工具被广泛应用。本研究介绍了两只临床和神经系统表现进行性的狗,在发现症状(第一例为头部倾斜和盘旋,第二例为癫痫发作和共济失调)后,建议进行MRI评估。影像学检查显示肿块存在,位于第三脑室,在常规序列T1, T2和FLAIR中具有异质性信号改变。将影像学资料与组织病理学检查相结合,可以确定两例脉络膜丛癌的诊断。
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引用次数: 0
Does a Good Concentration Involve Necessarily a Better Use of the Production Factors? 良好的集中是否必然意味着更好地利用生产要素?
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0034
I. Vlad, E. Toma
Abstract The paper was based on the idea that important changes have been occurred in the last years in the Romanian agriculture. Labour is one of the most important production factor in rural area and when this is linked to the agriculture sector, becomes a strategic issue. Land as one of the most limited resources in agriculture plays also an important role, its degree of concentration determining the structure of the farms system. The purpose of the study was to explore, by means of the Gini coefficient, whether the labour, in different forms (paid, unpaid labour) follows a similar degree of concentration as the Total Utilized Agricultural Area (UAA) and the farm output. The datasets used for the paper were the FADN public database (period 2007-2015) and the Tempo online dataset from Romanian National Institute of Statistics. The findings of this analyse were that paid labour, unpaid labour and the output followed a decreased trend of concentration and become during the analysed period, more equal distributed, while the area tends to have more unequal distribution, so the large farms tends to become larger.
摘要本文是基于的想法,重要的变化已经发生在罗马尼亚农业在过去几年。劳动力是农村地区最重要的生产要素之一,当它与农业部门联系起来时,就成为一个战略问题。土地作为农业中最有限的资源之一,也起着重要的作用,其集中度决定着农业系统的结构。本研究的目的是通过基尼系数探讨不同形式的劳动(有偿劳动和无偿劳动)是否与农业总利用面积(UAA)和农业产出具有相似的集中程度。本文使用的数据集是FADN公共数据库(2007-2015年)和罗马尼亚国家统计局的Tempo在线数据集。分析发现,在分析期间,有偿劳动、无偿劳动和产出集中度呈下降趋势,分布更加均匀,而面积分布更加不均匀,因此大农场趋向于变得更大。
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引用次数: 0
Enzyme Activity as an Indicator of Soil Quality in Sterile Waste Heap 酶活性作为无菌废物堆土壤质量的指标
Pub Date : 2018-07-01 DOI: 10.2478/alife-2018-0054
A. M. Dodocioiu, C. Babeanu, D. Buzatu
Abstract Recent research reports that enzymes accumulated in soil participate in the cycles of elements and contribute to soil fertility. The purpose of this paper is to determine the enzymatic activity and the humus content in the materials from waste heap in order to assess their fertility and to establish possible re-cultivation technologies. The investigations were carried out on materials from the Pesteana North waste heap. In order to achieve this, the activity of catalase, invertase, urease and phosphatase activity was determined in different plots of the heap during the summer and as a control the zonal soil was used. Enzymatic activities were colorimetric assay using appropriate substrates. The obtained results show a large variation in the values of the determined enzymatic activities. In all investigated plots, all enzymes are found to be lower than those determined for the zonal soil. A strong positive correlation is observed between enzymatic activities of catalase and invertase and the accumulation of humus which demonstrates that enzymatic activities has a useful tool for studying the evolution of heap materials to agricultural soils.
近年来的研究表明,土壤中积累的酶参与元素循环,对土壤肥力有贡献。本文的目的是测定废物堆中材料的酶活性和腐殖质含量,以评估其肥力并建立可能的再培养技术。调查是对来自Pesteana North废物堆的材料进行的。为了实现这一目标,在夏季测定了堆中不同地块的过氧化氢酶、转化酶、脲酶和磷酸酶活性,并使用地带性土壤作为对照。采用合适底物比色法测定酶活性。所得结果表明,测定的酶活性值变化很大。在所有调查样地中,发现所有酶都低于地带性土壤的测定值。过氧化氢酶和转化酶活性与腐殖质积累呈显著正相关,表明酶活性是研究堆料向农业土壤演化的有效工具。
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引用次数: 0
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