Pub Date : 2021-09-01DOI: 10.1186/s41476-021-00164-9
Gonca Bilge, Kemal Efe Eseller, Halil Berberoglu, Banu Sezer, Ugur Tamer, Ismail Hakki Boyaci
Laser induced breakdown spectroscopy (LIBS) is a rapid optical spectroscopy technique for elemental determination, which has been used for quantitative analysis in many fields. However, the calibration involving atomic emission intensity and sample concentration, is still a challenge due to physical-chemical matrix effect of samples and fluctuations of experimental parameters. To overcome these problems, various chemometric data analysis techniques have been combined with LIBS technique. In this study, LIBS was used to show its potential as a routine analysis for Na measurements in bakery products. A series of standard bread samples containing various concentrations of NaCl (0.025%–3.5%) was prepared to compare different calibration techniques. Standard calibration curve (SCC), artificial neural network (ANN) and partial least square (PLS) techniques were used as calibration strategies. Among them, PLS was found to be more efficient for predicting the Na concentrations in bakery products with an increase in coefficient of determination value from 0.961 to 0.999 for standard bread samples and from 0.788 to 0.943 for commercial products.
{"title":"Comparison of different calibration techniques of laser induced breakdown spectroscopy in bakery products: on NaCl measurement","authors":"Gonca Bilge, Kemal Efe Eseller, Halil Berberoglu, Banu Sezer, Ugur Tamer, Ismail Hakki Boyaci","doi":"10.1186/s41476-021-00164-9","DOIUrl":"10.1186/s41476-021-00164-9","url":null,"abstract":"<div><p>Laser induced breakdown spectroscopy (LIBS) is a rapid optical spectroscopy technique for elemental determination, which has been used for quantitative analysis in many fields. However, the calibration involving atomic emission intensity and sample concentration, is still a challenge due to physical-chemical matrix effect of samples and fluctuations of experimental parameters. To overcome these problems, various chemometric data analysis techniques have been combined with LIBS technique. In this study, LIBS was used to show its potential as a routine analysis for Na measurements in bakery products. A series of standard bread samples containing various concentrations of NaCl (0.025%–3.5%) was prepared to compare different calibration techniques. Standard calibration curve (SCC), artificial neural network (ANN) and partial least square (PLS) techniques were used as calibration strategies. Among them, PLS was found to be more efficient for predicting the Na concentrations in bakery products with an increase in coefficient of determination value from 0.961 to 0.999 for standard bread samples and from 0.788 to 0.943 for commercial products.</p></div>","PeriodicalId":674,"journal":{"name":"Journal of the European Optical Society-Rapid Publications","volume":"17 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://jeos.springeropen.com/counter/pdf/10.1186/s41476-021-00164-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138514733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-11DOI: 10.1186/s41476-021-00162-x
Feng-Xuan Zhang, Guo-Yang Wang, Hai-Yun Huang, Meng-Han Chen, Si-Jia Zhang, Bo Su, Cun-Lin Zhang
The vibrational energy levels of many biomolecules correspond to the terahertz band; thus, terahertz technology can be used to identify these substances. Moreover, as the biological activity of most biomolecules can be observed only in aqueous solution, the characteristics of such biomolecules must be studied in aqueous solution. In this study, a simple microfluidic chip, a temperature control device and a strong electric field device were designed to study the terahertz absorption characteristics of guar gum for different temperatures, concentrations and electric field exposure durations, thus enabling the use of terahertz technology to analyse the characteristics of guar gum.
{"title":"Terahertz absorption characteristics of guar gum determined via microfluidic technology","authors":"Feng-Xuan Zhang, Guo-Yang Wang, Hai-Yun Huang, Meng-Han Chen, Si-Jia Zhang, Bo Su, Cun-Lin Zhang","doi":"10.1186/s41476-021-00162-x","DOIUrl":"10.1186/s41476-021-00162-x","url":null,"abstract":"<div><p>The vibrational energy levels of many biomolecules correspond to the terahertz band; thus, terahertz technology can be used to identify these substances. Moreover, as the biological activity of most biomolecules can be observed only in aqueous solution, the characteristics of such biomolecules must be studied in aqueous solution. In this study, a simple microfluidic chip, a temperature control device and a strong electric field device were designed to study the terahertz absorption characteristics of guar gum for different temperatures, concentrations and electric field exposure durations, thus enabling the use of terahertz technology to analyse the characteristics of guar gum.</p></div>","PeriodicalId":674,"journal":{"name":"Journal of the European Optical Society-Rapid Publications","volume":"17 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2021-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://jeos.springeropen.com/counter/pdf/10.1186/s41476-021-00162-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43339647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-08-03DOI: 10.1186/s41476-021-00163-w
Xi Peng, Yixin Yan
Two-dimensional materials are widely used in a variety of fields, such as medical treatment, industrial preparation, machining, etc. In this review, we have made a detailed description of the development of fiber lasers as well as the evolution of two-dimensional materials, especially graphene. In addition, we describe the optical properties of graphene and its preparations, for instance, chemical exfoliatio, liquid phase exfoliation, electrochemical technique, chemical vapor deposition, supercritical fluid exfoliation, and thermal exfoliation. Meanwhile, we also summarized several types of graphene saturable absorbers like all fiber, D-shaped, and optical deposition. Furthermore, we summarize the optical applications of fiber lasers based on graphene. Finally, we also take a look at the future perspectives of graphene and discuss the future applications of graphene in the field of optics. It is note worth that future fiber lasers will use more heterostructures or gas-solid mixtures to prepare saturable absorbers.
{"title":"Graphene saturable absorbers applications in fiber lasers","authors":"Xi Peng, Yixin Yan","doi":"10.1186/s41476-021-00163-w","DOIUrl":"10.1186/s41476-021-00163-w","url":null,"abstract":"<div><p>Two-dimensional materials are widely used in a variety of fields, such as medical treatment, industrial preparation, machining, etc. In this review, we have made a detailed description of the development of fiber lasers as well as the evolution of two-dimensional materials, especially graphene. In addition, we describe the optical properties of graphene and its preparations, for instance, chemical exfoliatio, liquid phase exfoliation, electrochemical technique, chemical vapor deposition, supercritical fluid exfoliation, and thermal exfoliation. Meanwhile, we also summarized several types of graphene saturable absorbers like all fiber, D-shaped, and optical deposition. Furthermore, we summarize the optical applications of fiber lasers based on graphene. Finally, we also take a look at the future perspectives of graphene and discuss the future applications of graphene in the field of optics. It is note worth that future fiber lasers will use more heterostructures or gas-solid mixtures to prepare saturable absorbers.</p></div>","PeriodicalId":674,"journal":{"name":"Journal of the European Optical Society-Rapid Publications","volume":"17 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2021-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://jeos.springeropen.com/counter/pdf/10.1186/s41476-021-00163-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138514734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-26DOI: 10.1186/s41476-021-00161-y
Liangxin Yang, Irfan Badar, Christian Hellmann, Frank Wyrowski
In far-field light shaping, one of the design methods is based on a one-to-one map between the irradiance of the source and target. However, an integrability issue may occur in this kind of algorithms, either in the ray mapping method for designing a freeform surface or in those geometric-optics-based methods for achieving a required output phase. We introduce another mapping-type algorithm to tackle the integrability problem, which instead of establishing a mapping between both the source and target irradiance in the space domain, the mapping is assumed on electric fields of a Fourier pair between the space domain and the spatial-frequency domain. By solving the mapping from the Fourier pair, the gradient of the output phase is achieved, that the gradient is equivalent to the obtained mapping function. Moreover, the existence and the characterization of the mapping guarantees the integrability of the gradient so that a smooth output phase can be directly integrated. Based on the obtained smooth output phase, a freeform surface can then be designed for the light-shaping task. Numerical examples are demonstrated for the comparison of the approaches with different mapping assumptions.
{"title":"Integrable solution for light shaping based on a Fourier-pair mapping","authors":"Liangxin Yang, Irfan Badar, Christian Hellmann, Frank Wyrowski","doi":"10.1186/s41476-021-00161-y","DOIUrl":"10.1186/s41476-021-00161-y","url":null,"abstract":"<div><p>In far-field light shaping, one of the design methods is based on a one-to-one map between the irradiance of the source and target. However, an integrability issue may occur in this kind of algorithms, either in the ray mapping method for designing a freeform surface or in those geometric-optics-based methods for achieving a required output phase. We introduce another mapping-type algorithm to tackle the integrability problem, which instead of establishing a mapping between both the source and target irradiance in the space domain, the mapping is assumed on electric fields of a Fourier pair between the space domain and the spatial-frequency domain. By solving the mapping from the Fourier pair, the gradient of the output phase is achieved, that the gradient is equivalent to the obtained mapping function. Moreover, the existence and the characterization of the mapping guarantees the integrability of the gradient so that a smooth output phase can be directly integrated. Based on the obtained smooth output phase, a freeform surface can then be designed for the light-shaping task. Numerical examples are demonstrated for the comparison of the approaches with different mapping assumptions.</p></div>","PeriodicalId":674,"journal":{"name":"Journal of the European Optical Society-Rapid Publications","volume":"17 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2021-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://jeos.springeropen.com/counter/pdf/10.1186/s41476-021-00161-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138514732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-23DOI: 10.21203/RS.3.RS-660206/V1
R. Shurvinton, F. Lemarchand, A. Moreau, J. Lumeau
A precise spectrophotometric method to determine the refractive index of a semitransparent metallic thin film is presented. This method relies on interference enhancement of the measured spectra, employing an opaque substrate with a dielectric spacer layer beneath the absorbing layer of interest to create interference fringes.The resulting spectral oscillations of the stack are highly sensitive to the properties of the top absorbing layer, allowing precise determination of the refractive index via fitting. The performance of this method is verified using simulations in comparison to the typical method of depositing the absorbing thin film directly onto a transparent substrate. An experimental demonstration is made for titanium thin films over the visible range (370-835 nm). The refractive index of these films is extracted from experimental data using a combination of the Modified Drude and Forouhi-Bloomer models. This method showed high repeatability and precision, and is verified for Ti films between 6-70 nm thickness.
{"title":"Precise spectrophotometric method for semitransparent metallic thin-film index determination using interference enhancement","authors":"R. Shurvinton, F. Lemarchand, A. Moreau, J. Lumeau","doi":"10.21203/RS.3.RS-660206/V1","DOIUrl":"https://doi.org/10.21203/RS.3.RS-660206/V1","url":null,"abstract":"A precise spectrophotometric method to determine the refractive index of a semitransparent metallic thin film is presented. This method relies on interference enhancement of the measured spectra, employing an opaque substrate with a dielectric spacer layer beneath the absorbing layer of interest to create interference fringes.The resulting spectral oscillations of the stack are highly sensitive to the properties of the top absorbing layer, allowing precise determination of the refractive index via fitting. The performance of this method is verified using simulations in comparison to the typical method of depositing the absorbing thin film directly onto a transparent substrate. An experimental demonstration is made for titanium thin films over the visible range (370-835 nm). The refractive index of these films is extracted from experimental data using a combination of the Modified Drude and Forouhi-Bloomer models. This method showed high repeatability and precision, and is verified for Ti films between 6-70 nm thickness.","PeriodicalId":674,"journal":{"name":"Journal of the European Optical Society-Rapid Publications","volume":"17 1","pages":"1-14"},"PeriodicalIF":1.5,"publicationDate":"2021-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44389875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-10DOI: 10.1186/s41476-021-00159-6
Xuexing Li, Wenhui Zhang
Binary defocusing technique can effectively break the limitation of hardware speed, which has been widely used in the real-time three-dimensional (3D) reconstruction. In addition, fusion technique can reduce captured images count for a 3D scene, which helps to improve real-time performance. Unfortunately, it is difficult for binary defocusing technique and fusion technique working simultaneously. To this end, our research established a novel system framework consisting of dual projectors and a camera, where the position and posture of the dual projectors are not strictly required. And, the dual projectors can adjust defocusing level independently. Based on this, this paper proposed a complementary decoding method with unconstrained dual projectors. The core idea is that low-resolution information is employed for high-resolution phase unwrapping. For this purpose, we developed the low-resolution depth extraction strategy based on periodic space-time coding patterns and the method from the low-resolution order to high-resolution order of fringe. Finally, experimental results demonstrated the performance of our proposed method, and the proposed method only requires three images for a 3D scene, as well as has strong robustness, expansibility, and implementation.
{"title":"Binary defocusing technique based on complementary decoding with unconstrained dual projectors","authors":"Xuexing Li, Wenhui Zhang","doi":"10.1186/s41476-021-00159-6","DOIUrl":"https://doi.org/10.1186/s41476-021-00159-6","url":null,"abstract":"<p>Binary defocusing technique can effectively break the limitation of hardware speed, which has been widely used in the real-time three-dimensional (3D) reconstruction. In addition, fusion technique can reduce captured images count for a 3D scene, which helps to improve real-time performance. Unfortunately, it is difficult for binary defocusing technique and fusion technique working simultaneously. To this end, our research established a novel system framework consisting of dual projectors and a camera, where the position and posture of the dual projectors are not strictly required. And, the dual projectors can adjust defocusing level independently. Based on this, this paper proposed a complementary decoding method with unconstrained dual projectors. The core idea is that low-resolution information is employed for high-resolution phase unwrapping. For this purpose, we developed the low-resolution depth extraction strategy based on periodic space-time coding patterns and the method from the low-resolution order to high-resolution order of fringe. Finally, experimental results demonstrated the performance of our proposed method, and the proposed method only requires three images for a 3D scene, as well as has strong robustness, expansibility, and implementation.</p>","PeriodicalId":674,"journal":{"name":"Journal of the European Optical Society-Rapid Publications","volume":"17 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4423611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-30DOI: 10.1186/s41476-021-00154-x
S. Coëtmellec, D. Lebrun, M. Brunel, A. J. E. M. Janssen
In this publication, the Luneberg integrals are revisited and the conditions of the using of such integrals have been recalled. Additivity law of Luneberg’s integrals and the link with the Frenel kernel for the propagation are discussed. By means of the definition of the Luneberg’s integrals, the propagation of a vectorial electromagnetic field (Hertz potentials) is developed and a new approach of the computation have been proposed based on Zernike polynomials. With this new approach simulations of holograms is illustrated in the case of the digital in-line holography with an opaque disk.
{"title":"Assessment about Luneberg integrals and application to digital in-line holography","authors":"S. Coëtmellec, D. Lebrun, M. Brunel, A. J. E. M. Janssen","doi":"10.1186/s41476-021-00154-x","DOIUrl":"https://doi.org/10.1186/s41476-021-00154-x","url":null,"abstract":"<p>In this publication, the Luneberg integrals are revisited and the conditions of the using of such integrals have been recalled. Additivity law of Luneberg’s integrals and the link with the Frenel kernel for the propagation are discussed. By means of the definition of the Luneberg’s integrals, the propagation of a vectorial electromagnetic field (Hertz potentials) is developed and a new approach of the computation have been proposed based on Zernike polynomials. With this new approach simulations of holograms is illustrated in the case of the digital in-line holography with an opaque disk.</p>","PeriodicalId":674,"journal":{"name":"Journal of the European Optical Society-Rapid Publications","volume":"17 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41476-021-00154-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5153079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-30DOI: 10.1186/s41476-021-00158-7
Jinren Yan
In this work, a scheme of combining an aspheric reflector and a primary packaging freeform lens in phosphor-converted lighting-emitting diode (pc-LED) based lighting system design was developed. In contrast to most of previous works in which the color and illumination uniformity of the pc-LED lighting systems were improved one by one, the developed scheme can improve both indicators simultaneously. To demonstrate the feasibility of the developed design scheme, an initially designed pc-LED optical model, with an average correlated color temperature (CCT) of about 5000?K, was constructed as the light source of the illumination system. The key ingredient to the color uniformity of the illumination system was investigated at first, and then several instances with the cut-off angle ranging from 40 to 60 degrees were further discussed. Detailed analysis shows: for each case, the CCT variation decreases from over 4000?K to smaller than 300?K and the illumination uniformity increases from a very low level to better than 80% on the target region.
{"title":"Design of a phosphor-converted LED based lighting system considering both the lighting and color uniformity","authors":"Jinren Yan","doi":"10.1186/s41476-021-00158-7","DOIUrl":"https://doi.org/10.1186/s41476-021-00158-7","url":null,"abstract":"<p>In this work, a scheme of combining an aspheric reflector and a primary packaging freeform lens in phosphor-converted lighting-emitting diode (pc-LED) based lighting system design was developed. In contrast to most of previous works in which the color and illumination uniformity of the pc-LED lighting systems were improved one by one, the developed scheme can improve both indicators simultaneously. To demonstrate the feasibility of the developed design scheme, an initially designed pc-LED optical model, with an average correlated color temperature (CCT) of about 5000?K, was constructed as the light source of the illumination system. The key ingredient to the color uniformity of the illumination system was investigated at first, and then several instances with the cut-off angle ranging from 40 to 60 degrees were further discussed. Detailed analysis shows: for each case, the CCT variation decreases from over 4000?K to smaller than 300?K and the illumination uniformity increases from a very low level to better than 80% on the target region.</p>","PeriodicalId":674,"journal":{"name":"Journal of the European Optical Society-Rapid Publications","volume":"17 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41476-021-00158-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5159368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-16DOI: 10.1186/s41476-021-00157-8
M. Al-Rubaiee, A. H. Al-Janabi, S. C. Fleming, A. Argyros
One of the unique properties of metamaterials is the ability to manipulate electromagnetic waves at subwavelength scales, made possible by their structure on these scales. Here, rather than consider effective bulk properties, we consider the properties of microscopic features based on considering resonant unit cells. We used wire array metamaterials to form localized resonant cavities by changing the resonance frequency of one or more unit cells, surrounded by unchanged unit cells that do not support resonance for the propagating mode (i.e. forming a band gap). We validate our approach experimentally with electromagnetic waves in the terahertz range, demonstrating and characterizing subwavelength resonant cavities in this range. These resonant cavities can pave the way for ultra-compact subwavelength waveguides and other optical components.
{"title":"Metamaterials subwavelength terahertz resonant cavities","authors":"M. Al-Rubaiee, A. H. Al-Janabi, S. C. Fleming, A. Argyros","doi":"10.1186/s41476-021-00157-8","DOIUrl":"https://doi.org/10.1186/s41476-021-00157-8","url":null,"abstract":"<p>One of the unique properties of metamaterials is the ability to manipulate electromagnetic waves at subwavelength scales, made possible by their structure on these scales. Here, rather than consider effective bulk properties, we consider the properties of microscopic features based on considering resonant unit cells. We used wire array metamaterials to form localized resonant cavities by changing the resonance frequency of one or more unit cells, surrounded by unchanged unit cells that do not support resonance for the propagating mode (i.e. forming a band gap). We validate our approach experimentally with electromagnetic waves in the terahertz range, demonstrating and characterizing subwavelength resonant cavities in this range. These resonant cavities can pave the way for ultra-compact subwavelength waveguides and other optical components.</p>","PeriodicalId":674,"journal":{"name":"Journal of the European Optical Society-Rapid Publications","volume":"17 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41476-021-00157-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4941719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-16DOI: 10.1186/s41476-021-00155-w
Liron Zada, Bart Fokker, Heather A. Leslie, A. Dick Vethaak, Johannes F. de Boer, Freek Ariese
Two simulation programs of a stimulated Raman scattering microscopy (SRS) imaging system with lock-in amplifier (LIA) detection were developed. SRS is an imaging technique based on the vibrational Raman cross-section as the contrast mechanism and enables fast, label-free imaging. Most SRS implementations are based on LIA detection of a modulated signal. However, building and operating such SRS set-ups still poses a challenge when selecting the LIA parameter settings for optimized acquisition speed or image quality. Moreover, the type of sample, e.g. a sparse sample vs. a densely packed sample, the required resolution as well as the Raman cross-section and the laser powers affect the parameter choice.
A simulation program was used to find these optimal parameters. The focal spot diameters of the individual lasers (pump and Stokes) were used to estimate the effective SRS signal focal spot and the (optical) spatial resolution. By calibrating the signal and noise propagation through an SRS system for a known molecule, we estimated the signal and noise input to the LIA. We used a low pass filter model to simulate the LIA behavior in order to find the optimal parameters (i.e. filter order and time constant).
Optimization was done for either image quality (expressed as contrast to noise ratio) or acquisition time. The targeted object size was first determined as a measure for the required resolution. The simulation output consisted of the LIA parameters, pixel dwell time and contrast to noise ratio.
In a second simulation we evaluated SRS imaging based on the same principles as the optimal setting simulation, i.e. the signals were propagated through an imaging system and LIA detection. The simulated images were compared to experimental SRS images of polystyrene beads.
Finally, the same software was used to simulate multiplexed SRS imaging. In this study we modeled a six-channel frequency-encoded multiplexed SRS system demodulated with six LIA channels. We evaluated the inter-channel crosstalk as a function of chosen LIA parameters, which in multiplex SRS imaging also needs to be considered.
These programs to optimize the contrast to noise ratio, acquisition speed, resolution and crosstalk will be useful for operating stimulated Raman scattering imaging setup, as well as for designing novel setups.
{"title":"Stimulated Raman scattering simulation for imaging optimization","authors":"Liron Zada, Bart Fokker, Heather A. Leslie, A. Dick Vethaak, Johannes F. de Boer, Freek Ariese","doi":"10.1186/s41476-021-00155-w","DOIUrl":"https://doi.org/10.1186/s41476-021-00155-w","url":null,"abstract":"<p>Two simulation programs of a stimulated Raman scattering microscopy (SRS) imaging system with lock-in amplifier (LIA) detection were developed. SRS is an imaging technique based on the vibrational Raman cross-section as the contrast mechanism and enables fast, label-free imaging. Most SRS implementations are based on LIA detection of a modulated signal. However, building and operating such SRS set-ups still poses a challenge when selecting the LIA parameter settings for optimized acquisition speed or image quality. Moreover, the type of sample, e.g. a sparse sample vs. a densely packed sample, the required resolution as well as the Raman cross-section and the laser powers affect the parameter choice.</p><p>A simulation program was used to find these optimal parameters. The focal spot diameters of the individual lasers (pump and Stokes) were used to estimate the effective SRS signal focal spot and the (optical) spatial resolution. By calibrating the signal and noise propagation through an SRS system for a known molecule, we estimated the signal and noise input to the LIA. We used a low pass filter model to simulate the LIA behavior in order to find the optimal parameters (i.e. filter order and time constant).</p><p>Optimization was done for either image quality (expressed as contrast to noise ratio) or acquisition time. The targeted object size was first determined as a measure for the required resolution. The simulation output consisted of the LIA parameters, pixel dwell time and contrast to noise ratio.</p><p>In a second simulation we evaluated SRS imaging based on the same principles as the optimal setting simulation, i.e. the signals were propagated through an imaging system and LIA detection. The simulated images were compared to experimental SRS images of polystyrene beads.</p><p>Finally, the same software was used to simulate multiplexed SRS imaging. In this study we modeled a six-channel frequency-encoded multiplexed SRS system demodulated with six LIA channels. We evaluated the inter-channel crosstalk as a function of chosen LIA parameters, which in multiplex SRS imaging also needs to be considered.</p><p>These programs to optimize the contrast to noise ratio, acquisition speed, resolution and crosstalk will be useful for operating stimulated Raman scattering imaging setup, as well as for designing novel setups.</p>","PeriodicalId":674,"journal":{"name":"Journal of the European Optical Society-Rapid Publications","volume":"17 1","pages":""},"PeriodicalIF":1.5,"publicationDate":"2021-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s41476-021-00155-w","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4941720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}