Pub Date : 2010-03-30DOI: 10.3724/SP.J.1143.2009.09036
Gao Lixia, L. Nian, Huang BangHai
In this study,using a pseudo-cross strategy,we constructed two maps of Hedyhium,Hedychium coronarium and Hedychium forrestii based on SRAP markers. The mapping population consisted of 87 progenies,from a F1 population. A total of 414 primer pairs were screened and 92 pairs were considered. Among 398 loci,237 loci were from the paternal parent and 161 were from the maternal parent. After χ2 test and linkage analysis,two maps were constructed as following:The paternal parent contained 203 loci and spanned 1 386.8 cm,which spread over 23 linkage groups. The maternal parent contained 139 loci and spanned 917.1 cm. These loci were distributed in 18 linkage groups.
{"title":"Linkage Maps of the Genus Hedychium (Zingiberaceae) Based on SRAP","authors":"Gao Lixia, L. Nian, Huang BangHai","doi":"10.3724/SP.J.1143.2009.09036","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09036","url":null,"abstract":"In this study,using a pseudo-cross strategy,we constructed two maps of Hedyhium,Hedychium coronarium and Hedychium forrestii based on SRAP markers. The mapping population consisted of 87 progenies,from a F1 population. A total of 414 primer pairs were screened and 92 pairs were considered. Among 398 loci,237 loci were from the paternal parent and 161 were from the maternal parent. After χ2 test and linkage analysis,two maps were constructed as following:The paternal parent contained 203 loci and spanned 1 386.8 cm,which spread over 23 linkage groups. The maternal parent contained 139 loci and spanned 917.1 cm. These loci were distributed in 18 linkage groups.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"87 1","pages":"317-325"},"PeriodicalIF":0.0,"publicationDate":"2010-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85931907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-30DOI: 10.3724/SP.J.1143.2009.09048
Pi Qiuxia, Y. Ning, H. Hong, Liang Shuyun
{"title":"Flower development and cultivation of Paphiopedilum armeniacum (Orchidaceae).","authors":"Pi Qiuxia, Y. Ning, H. Hong, Liang Shuyun","doi":"10.3724/SP.J.1143.2009.09048","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09048","url":null,"abstract":"","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"25 1","pages":"296-302"},"PeriodicalIF":0.0,"publicationDate":"2010-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85667433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-30DOI: 10.3724/SP.J.1143.2009.09075
Z. Nie, J. Wen, Hang Sun
{"title":"AFLP Analysis of Phryma (Phrymaceae) Disjunct between Eastern Asia and Eastern North America: AFLP Analysis of Phryma (Phrymaceae) Disjunct between Eastern Asia and Eastern North America","authors":"Z. Nie, J. Wen, Hang Sun","doi":"10.3724/SP.J.1143.2009.09075","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09075","url":null,"abstract":"","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"132 4","pages":"289-295"},"PeriodicalIF":0.0,"publicationDate":"2010-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72627418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-30DOI: 10.3724/SP.J.1143.2009.09017
Tian Xuejun, Tao Hong-zheng, Luo Jing, Zhang Xudong, Tao FaQing
{"title":"Physiological effect of heat stress on pea (Pisum sativum) hypocotyls.","authors":"Tian Xuejun, Tao Hong-zheng, Luo Jing, Zhang Xudong, Tao FaQing","doi":"10.3724/SP.J.1143.2009.09017","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09017","url":null,"abstract":"","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"43 1","pages":"363-368"},"PeriodicalIF":0.0,"publicationDate":"2010-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89396428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-01-01DOI: 10.3724/sp.j.1143.2010.00032
Chenfei Lu
The complete gene of cytosolic malate dehydrogenase (cMDH) from Camellia sinensis,called Cs-cMDH,was obtained by RT-PCR and rapid amplification of cDNA ends (GenBank accession number GQ845406). This gene was 1 235 bp in length,encoding a protein of 332 amino acids with the putative molecular weight of 35.5 kD. The E.coli Rosetta (DE3) harboring pGEX-MDH was induced by 0.5 mmol·L-1 IPTG at 32℃ for 3 hours,and a 61.5 kD glutathione Stransferase (GST)-fused MDH was obtained in soluble form. The results of NCBI-BLAST revealed that Cs-cMDH shared 88%-93% of amino acid sequence identity with other cMDH from different higher plants. According to the multiple sequence alignment based on the three-dimensional structure of protein,Cs-cMDH was predicted to be a dimer with thirteen β-sheet and thirteen α-helix of each subunit. Cs-cMDH contains typical fingerprint sequence (G12AAGQIG18) as all MDHs. The amino acid D43 in Cs-cMDH is conserved in all NAD-MDHs. Cs-cMDH also has some conserved sequence units homologous to other NAD-MDHs,such as NAD+ binding sites,catalytic motif and substrate binding sites. Moreover,Cs-cMDH contains six Cys which are highly conserved in all plant NAD-cMDHs. Therefore,Cs-cMDH was inferred to be NAD-dependent cMDH. The present study may provide the fundament for the further functional characterization of Cs-cMDH.
{"title":"Prokaryotic Expression and Bioinformatics Analysis of Cytosolic Malate Dehydrogenase from Camellia sinensis(Theaceae)","authors":"Chenfei Lu","doi":"10.3724/sp.j.1143.2010.00032","DOIUrl":"https://doi.org/10.3724/sp.j.1143.2010.00032","url":null,"abstract":"The complete gene of cytosolic malate dehydrogenase (cMDH) from Camellia sinensis,called Cs-cMDH,was obtained by RT-PCR and rapid amplification of cDNA ends (GenBank accession number GQ845406). This gene was 1 235 bp in length,encoding a protein of 332 amino acids with the putative molecular weight of 35.5 kD. The E.coli Rosetta (DE3) harboring pGEX-MDH was induced by 0.5 mmol·L-1 IPTG at 32℃ for 3 hours,and a 61.5 kD glutathione Stransferase (GST)-fused MDH was obtained in soluble form. The results of NCBI-BLAST revealed that Cs-cMDH shared 88%-93% of amino acid sequence identity with other cMDH from different higher plants. According to the multiple sequence alignment based on the three-dimensional structure of protein,Cs-cMDH was predicted to be a dimer with thirteen β-sheet and thirteen α-helix of each subunit. Cs-cMDH contains typical fingerprint sequence (G12AAGQIG18) as all MDHs. The amino acid D43 in Cs-cMDH is conserved in all NAD-MDHs. Cs-cMDH also has some conserved sequence units homologous to other NAD-MDHs,such as NAD+ binding sites,catalytic motif and substrate binding sites. Moreover,Cs-cMDH contains six Cys which are highly conserved in all plant NAD-cMDHs. Therefore,Cs-cMDH was inferred to be NAD-dependent cMDH. The present study may provide the fundament for the further functional characterization of Cs-cMDH.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78513250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-01-01DOI: 10.3724/sp.j.1143.2010.09234
Z. Li
We compared the performances of the candidate loci for moss DNA barcoding and the primers used in amplifying the loci. Primers for three coded loci (matK,rps4 and rbcL-a) and four non-coded loci (atpB-rbcL,atpF-H,psbK-I and trnH-psbA) of the chloroplast genome,one from the mitochondrial genome (nad5),and one from the nucleus genome (ITS2) were evaluated. Seventy-four samples representing 14 species belonging to five genera of Trachypodoaceae (or Meteoriaceae) were screened. All primers for matK and a pair of primers for trnH-psbA failed. Low successes were encountered with the primers for atpF-H and psbK-I. The primers for psbK-I produced several bands and the PCR products of atpF-H were difficult to sequence. The powers of the remaining six loci were compared using the variability,identification success and the resolutions. It was found that ITS2 is the most promising candidate for DNA barcoding for mosses. Among the chloroplast genes,atpB-rbcL exhibited the highest resolution. Although trnH-psbA is very variable,it is too short to be an ideal barcode alone. Combinations of chloroplast genes were also tried and Ps of both atpB-rbcL+trnH-psbA and rbcL-a++trnH-psbA were 64% using NJ method. More additions of loci did not increase the resolution. No barcoding gap exists for all these loci. Phylogenetic analyses were carried out prior to the DNA barcoding evaluation and some taxonomic problems do exist. This study exemplifies the necessity of correct species delimitation and the adoption of both plastid and nuclear loci in plant DNA barcoding.
{"title":"The Preliminary Study on DNA Barcoding of Mosses——A Case of Part of Genera of Meteoriaceae","authors":"Z. Li","doi":"10.3724/sp.j.1143.2010.09234","DOIUrl":"https://doi.org/10.3724/sp.j.1143.2010.09234","url":null,"abstract":"We compared the performances of the candidate loci for moss DNA barcoding and the primers used in amplifying the loci. Primers for three coded loci (matK,rps4 and rbcL-a) and four non-coded loci (atpB-rbcL,atpF-H,psbK-I and trnH-psbA) of the chloroplast genome,one from the mitochondrial genome (nad5),and one from the nucleus genome (ITS2) were evaluated. Seventy-four samples representing 14 species belonging to five genera of Trachypodoaceae (or Meteoriaceae) were screened. All primers for matK and a pair of primers for trnH-psbA failed. Low successes were encountered with the primers for atpF-H and psbK-I. The primers for psbK-I produced several bands and the PCR products of atpF-H were difficult to sequence. The powers of the remaining six loci were compared using the variability,identification success and the resolutions. It was found that ITS2 is the most promising candidate for DNA barcoding for mosses. Among the chloroplast genes,atpB-rbcL exhibited the highest resolution. Although trnH-psbA is very variable,it is too short to be an ideal barcode alone. Combinations of chloroplast genes were also tried and Ps of both atpB-rbcL+trnH-psbA and rbcL-a++trnH-psbA were 64% using NJ method. More additions of loci did not increase the resolution. No barcoding gap exists for all these loci. Phylogenetic analyses were carried out prior to the DNA barcoding evaluation and some taxonomic problems do exist. This study exemplifies the necessity of correct species delimitation and the adoption of both plastid and nuclear loci in plant DNA barcoding.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78548064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Two invasive species,Gymnocoronis spilanthoides and Cyclospermum leptophyllum,as well as two new distribution species,Primula nutantiflora and Allium aciphyllum are recorded for the first time from Yunnan Province. The harmfulness and controlling of Gymnocoronis spilanthoides and Cyclospermum leptophyllum are also disscussed in this paper.
{"title":"New Invasive and New Distribution Species of Spermatophyte in Yunnan","authors":"W. Huan","doi":"10.3724/sp.j.1143.2010.","DOIUrl":"https://doi.org/10.3724/sp.j.1143.2010.","url":null,"abstract":"Two invasive species,Gymnocoronis spilanthoides and Cyclospermum leptophyllum,as well as two new distribution species,Primula nutantiflora and Allium aciphyllum are recorded for the first time from Yunnan Province. The harmfulness and controlling of Gymnocoronis spilanthoides and Cyclospermum leptophyllum are also disscussed in this paper.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75862470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-01-01DOI: 10.3724/sp.j.1143.2010.00001
Y. Jun
We focused on the systematic positions and relationships of three sections of Camellia subgen.Thea of Theaceae,i.e.,sect.Longipedicellata,sect.Chrysantha (golden camellias) and sect.Longissima by using four chloroplast DNA regions (rpl16,psbA-trnH,trnL-F rpl32-trnL). We sampled 28 species representing four subgenus,11 sections in Camellia and two outgroups. Combined analyses of chloroplast DNA sequence data sets are performed with the neighbor-joining,maximum-parsimony and Bayesian inference methods,and the gene trees are constructed. The topologies of gene trees revealed that:1) sect. Chrysantha is paraphyletic or polyphyletic,containing three parallel lineages and Camellia longipedicellata (type of sect.Longipedicellata) nested inside; 2) all four species from Vietnam,together with C. longipedicellata,forming a well-supported monophyletic clade,which implies the close relationship between sect.Longipedicellata and sect.Chrysantha; and 3) sect.Longissima is not monophyletic because C.hekouensis is the sister to the rest of Camellia species. The systematic position of C.longissima and the relationship between sect.Longissima and sect.Longipedicellata are unsolved.
{"title":"Phylogeny of Camellia sects. Longipedicellata,Chrysantha and Longissima(Theaceae) Based on Sequence Data of Four Chloroplast DNA Loci","authors":"Y. Jun","doi":"10.3724/sp.j.1143.2010.00001","DOIUrl":"https://doi.org/10.3724/sp.j.1143.2010.00001","url":null,"abstract":"We focused on the systematic positions and relationships of three sections of Camellia subgen.Thea of Theaceae,i.e.,sect.Longipedicellata,sect.Chrysantha (golden camellias) and sect.Longissima by using four chloroplast DNA regions (rpl16,psbA-trnH,trnL-F rpl32-trnL). We sampled 28 species representing four subgenus,11 sections in Camellia and two outgroups. Combined analyses of chloroplast DNA sequence data sets are performed with the neighbor-joining,maximum-parsimony and Bayesian inference methods,and the gene trees are constructed. The topologies of gene trees revealed that:1) sect. Chrysantha is paraphyletic or polyphyletic,containing three parallel lineages and Camellia longipedicellata (type of sect.Longipedicellata) nested inside; 2) all four species from Vietnam,together with C. longipedicellata,forming a well-supported monophyletic clade,which implies the close relationship between sect.Longipedicellata and sect.Chrysantha; and 3) sect.Longissima is not monophyletic because C.hekouensis is the sister to the rest of Camellia species. The systematic position of C.longissima and the relationship between sect.Longissima and sect.Longipedicellata are unsolved.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74686591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-01-01DOI: 10.3724/sp.j.1143.2010.00087
Tang Gui
A new phenylpropanoid glucoside,caffeyl alcohol-3-O-b-D-glucopyranoside,together with nine known compounds,was isolated from the dry corms of Remusatia vivipara Schott. The structure of the new compound was determined by the spectroscopic method and acidic hydrolysis. The known compounds included three phenylpropanoids (coniferin,caffeyl alcohol and coniferyl alcohol),three neolignans [4,7,9,9'-tetrahydroxy-3,3'-dimethoxy-8-O-4'-neolign-7'-ene,(7R,8S)-D7'-3,3'-dimethoxy-4,7,9,9'-tetrahydroxy-8-O-4'-neolignan-7-O-B-D-glucopyranoside,and dehydrodiconiferyl alcohol-4-B-D-glucoside],an amide [(2E,4E)-N-isobutyl-2,4-decadienamide],a steroid saponin (methyl proto-taccaoside) and a triterpenoid saponin (saxifragifolin B ). All compounds were isolated from the genus Remusatia for the first time.
{"title":"A New Phenylpropanoid Glucoside from Remusatia vivipara(Araceae)","authors":"Tang Gui","doi":"10.3724/sp.j.1143.2010.00087","DOIUrl":"https://doi.org/10.3724/sp.j.1143.2010.00087","url":null,"abstract":"A new phenylpropanoid glucoside,caffeyl alcohol-3-O-b-D-glucopyranoside,together with nine known compounds,was isolated from the dry corms of Remusatia vivipara Schott. The structure of the new compound was determined by the spectroscopic method and acidic hydrolysis. The known compounds included three phenylpropanoids (coniferin,caffeyl alcohol and coniferyl alcohol),three neolignans [4,7,9,9'-tetrahydroxy-3,3'-dimethoxy-8-O-4'-neolign-7'-ene,(7R,8S)-D7'-3,3'-dimethoxy-4,7,9,9'-tetrahydroxy-8-O-4'-neolignan-7-O-B-D-glucopyranoside,and dehydrodiconiferyl alcohol-4-B-D-glucoside],an amide [(2E,4E)-N-isobutyl-2,4-decadienamide],a steroid saponin (methyl proto-taccaoside) and a triterpenoid saponin (saxifragifolin B ). All compounds were isolated from the genus Remusatia for the first time.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"96 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77918211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-10-25DOI: 10.3724/SP.J.1143.2009.09096
Yang Mingzhi, Huang Xingqi, Zhang Hanbo, C. Shan-na, Yang Hongyu, Cheng Zaiquan
{"title":"Cloning and analyses of a dual specific serine/thronine protein kinase gene with high conservative and constitutive expression in Oryza (OsSTK).","authors":"Yang Mingzhi, Huang Xingqi, Zhang Hanbo, C. Shan-na, Yang Hongyu, Cheng Zaiquan","doi":"10.3724/SP.J.1143.2009.09096","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09096","url":null,"abstract":"","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"21 1","pages":"433-438"},"PeriodicalIF":0.0,"publicationDate":"2009-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77877355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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