Pub Date : 2010-03-30DOI: 10.3724/SP.J.1143.2009.09090
Wei Kai-fa, Jia Wensuo
{"title":"Analysis of gene expression and source-sink dynamics in transportation regulation of ABA signal accumulation.","authors":"Wei Kai-fa, Jia Wensuo","doi":"10.3724/SP.J.1143.2009.09090","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09090","url":null,"abstract":"","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"48 1","pages":"344-352"},"PeriodicalIF":0.0,"publicationDate":"2010-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76352214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-30DOI: 10.3724/SP.J.1143.2009.09035
Lin Meizhen, Deng Hua, Wei Dongmei, Tian HuiQiao
The nuclear DNA content of male and female gametes of tobacco(Nicotiana tabacum) was measured using DAPI stain and microspectrofluorimetric measurement. Tobacco pollen is bicellular at anthesis,containing a vegetative cell and a generative cell,which divides to form two sperm cells in a pollen tube. The nuclear DNA content of generative cell in a pollen tube was at 2C level and that of two sperm cells in a pollen tube,which elongated in the style,at 1C level. Two sperm cells began to synthesize DNA after both were released in the degenerated synergid,and the quantity of nuclear DNA in both sperm cells approached 2C level before both fusing with egg and central cells. During this process,the nuclear DNA content of egg cell also began to increase and approached 2C level before fusing with sperm cell. After male and female gamete fused,the nuclear DNA content of zygote reached 4C level. Therefore,the fusion of male and female gametes of tobacco was at G2 of cell cycle,and this fusion of fertilization belonged to G2 type. This result displayed the fertilization multiformity in angiosperms.
{"title":"Change of DNA Content in Male and Female Gametes of Tobacco(Nicotiana tabacum)","authors":"Lin Meizhen, Deng Hua, Wei Dongmei, Tian HuiQiao","doi":"10.3724/SP.J.1143.2009.09035","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09035","url":null,"abstract":"The nuclear DNA content of male and female gametes of tobacco(Nicotiana tabacum) was measured using DAPI stain and microspectrofluorimetric measurement. Tobacco pollen is bicellular at anthesis,containing a vegetative cell and a generative cell,which divides to form two sperm cells in a pollen tube. The nuclear DNA content of generative cell in a pollen tube was at 2C level and that of two sperm cells in a pollen tube,which elongated in the style,at 1C level. Two sperm cells began to synthesize DNA after both were released in the degenerated synergid,and the quantity of nuclear DNA in both sperm cells approached 2C level before both fusing with egg and central cells. During this process,the nuclear DNA content of egg cell also began to increase and approached 2C level before fusing with sperm cell. After male and female gamete fused,the nuclear DNA content of zygote reached 4C level. Therefore,the fusion of male and female gametes of tobacco was at G2 of cell cycle,and this fusion of fertilization belonged to G2 type. This result displayed the fertilization multiformity in angiosperms.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"109 1","pages":"303-308"},"PeriodicalIF":0.0,"publicationDate":"2010-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77452575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-30DOI: 10.3724/SP.J.1143.2009.09093
L. Du, N. Tan, Wenyan Xu, Lili Lou
Plant cyclopeptides are a large group of small molecular weight natural products, typically with 4-10 amino acids, isolated from the leaves, stem barks, roots, and seeds of a wide variety of plant species throughout the world. The peptides are present in many Chinese medicinal plants, and their potentials have not been well exploited because of the lack of knowledge in their biosynthetic origin and mechanism. The cyclopeptides often have complex chemical structures distinct from common polypeptides (proteins) that are non-cyclic and gene-coded. This review discussed the potential origin of the cyclopeptides and their possible biosynthetic mechanisms.
{"title":"Plant cyclopeptides and possible biosynthetic mechanisms.","authors":"L. Du, N. Tan, Wenyan Xu, Lili Lou","doi":"10.3724/SP.J.1143.2009.09093","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09093","url":null,"abstract":"Plant cyclopeptides are a large group of small molecular weight natural products, typically with 4-10 amino acids, isolated from the leaves, stem barks, roots, and seeds of a wide variety of plant species throughout the world. The peptides are present in many Chinese medicinal plants, and their potentials have not been well exploited because of the lack of knowledge in their biosynthetic origin and mechanism. The cyclopeptides often have complex chemical structures distinct from common polypeptides (proteins) that are non-cyclic and gene-coded. This review discussed the potential origin of the cyclopeptides and their possible biosynthetic mechanisms.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"34 1","pages":"374-382"},"PeriodicalIF":0.0,"publicationDate":"2010-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86433145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-30DOI: 10.3724/SP.J.1143.2009.09030
Chen Yan, Xie Zheng-sheng, Zheng Xiaolin, Wang Wencui
The soil heavy metal element absorption and enrichment of Carambola(Averrhoa carambola) were analied through the heavy metal elements content such as Cd,Pb,Cu,Hg,As,Cr of different types,different soil samples and different age trees of carambola in lianjiang green food base were investigated. The results showed that ① the soil pH≤5.50 which is acid soils and the heavy metal content of carambola orchard soil and fresh fruits were in conformity with the quality requirements of the production of green food;② Heavy metal absorption carambola in the soil were different with the the different heavy metal element and different kinds;③ The enrichment of Cd was the most than other elements of heavy metals and the concentration coefficient was 0.947. According to the sequence as enrichment coefficients,the order was Cd(0.947) Hg(0.098) Cu(0.023) Pb(0.003) As(0.001) = Cr(0.001) .
通过对连江绿色食品基地不同类型、不同土壤样品和不同树龄杨桃的Cd、Pb、Cu、Hg、as、Cr等重金属元素含量的调查，分析了杨桃(Averrhoa Carambola)对土壤重金属元素的吸收和富集。结果表明:①土壤pH≤5.50为酸性土壤，杨桃果园土壤和新鲜水果的重金属含量符合绿色食品生产的质量要求;②杨桃在土壤中的重金属吸收量随重金属元素的不同和种类的不同而不同;③重金属元素中Cd的富集量最大，浓度系数为0.947。从富集系数的顺序来看，富集系数为Cd(0.947) Hg(0.098) Cu(0.023) Pb(0.003) as (0.001) = Cr(0.001)。
{"title":"Effects of Carambola (Averrhoa carambola) plantation on heavy metal content in soil and heavy metal accumulation in fruits.","authors":"Chen Yan, Xie Zheng-sheng, Zheng Xiaolin, Wang Wencui","doi":"10.3724/SP.J.1143.2009.09030","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09030","url":null,"abstract":"The soil heavy metal element absorption and enrichment of Carambola(Averrhoa carambola) were analied through the heavy metal elements content such as Cd,Pb,Cu,Hg,As,Cr of different types,different soil samples and different age trees of carambola in lianjiang green food base were investigated. The results showed that ① the soil pH≤5.50 which is acid soils and the heavy metal content of carambola orchard soil and fresh fruits were in conformity with the quality requirements of the production of green food;② Heavy metal absorption carambola in the soil were different with the the different heavy metal element and different kinds;③ The enrichment of Cd was the most than other elements of heavy metals and the concentration coefficient was 0.947. According to the sequence as enrichment coefficients,the order was Cd(0.947) Hg(0.098) Cu(0.023) Pb(0.003) As(0.001) = Cr(0.001) .","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"17 1","pages":"369-373"},"PeriodicalIF":0.0,"publicationDate":"2010-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73670599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-30DOI: 10.3724/SP.J.1143.2009.09013
Zhou Guoyan, Yang Zhengan, Zhang Yinhua, Guo Fenggen, Zhou Xiaogang, Zhang Shaosong, Sun Mao-lin, Wu Shaoyun, Ding Yuemei
Cabbage(Brassica oleracea var.capitata) is one of the most popular and widely cultivated vegetable crops in the world. In this paper,BADH(betaine aldehyde dehydrogenase) gene derived from spinach was transformed into the genome of cabbage line 03079 mediated by Agrobacterium tumefaciens. To establish the ideal transformation platform,the main factors which affect the transformation efficiency were optimized through the orthogonal design of L9(34) ,including the type of infection medium,concentration of acetosyringone,the period of infection time and co-culture time. The results indicated that No. 9 was the best treatment combination,i.e,AA liquid medium was the optimal infection medium for the cabbage transformation,and co-culturing for 2 days after infection(20 min) would be favorable for the transformation. If supplemented with 200 μmol L-1 acetosyringone in infection medium,the transformation efficiency would be improved and the transformed plant regenaration ratio reached at 54.26%. Based on this optimal transformtion system,we obtained many transgenic plants,and PCR analysis using BADH gene primers and Southern blot analysis indicated that the BADH gene had been integrated into genome of cabbage. The BADH enzymes activity of transgenic plants were tested after treated with NaCl,drought-tolerance and PEG stress. The results showed that the average values of the activity of BADH enzymes,which varied from 2.1 U to 3.6 U per mg in transgenic plants,were 1 to 3 times higher than that of un-transgenic plants,and there was a significant difference between transgenic and un-transgenic plants using Duncan′s Multiple Range Test. Furthermore,the average value of relative electronic conductivity(varying from 16.2% to 32.6%) of transgenic plants were significantly lower than that of un-transgenic plants,which indicated that the protection ability of membrane penetration was enhanced when BADH activity increasing and the stress resistance of the transgenic plants was improved by introduction BADH gene into cabbage. At the same time,most of the transgenic cabbage plants were vigrously growing under drought 、salt and polyethylene glycol(PEG) stresses. The average height increasing percentage of transgenic plants were significantly higher than that of un-transgenic plants. Those results showed that the traits of drought or salt tolerance of transgenic cabbage plants were improved after transformed with BADH gene,and our transgenic plants would be used as fundamental germplasm for stress-tolerant breeding of cabbage.
白菜(Brassica oleracea var.capitata)是世界上最流行和广泛种植的蔬菜作物之一。本文利用根癌农杆菌介导，将源自菠菜的BADH(betaine aldehyde dehydrogenase，甜菜醛脱氢酶)基因转入白菜品系03079的基因组中。为建立理想的转化平台，通过L9(34)正交设计，对影响转化效率的主要因素:感染培养基类型、乙酰丁香酮浓度、感染时间、共培养时间进行优化。结果表明，9号为最佳处理组合;e、AA液体培养基是大白菜转化的最佳侵染培养基，侵染后共培养2 d (20 min)有利于转化。在侵染培养基中添加200 μmol L-1乙酰丁香酮可提高转化效率，转化植株再生率可达54.26%。基于该优化转化体系，获得了多株转基因植株，利用BADH基因引物进行PCR分析和Southern blot分析表明，BADH基因已被整合到白菜基因组中。对转基因植株在NaCl、抗旱和PEG胁迫下的BADH酶活性进行了检测。结果表明，转基因植株的BADH酶活性平均值为2.1 ~ 3.6 U / mg，是未转基因植株的1 ~ 3倍，且采用Duncan’s多重极差检验，转基因植株与未转基因植株之间存在显著性差异。此外，转基因植株的平均相对电导率(16.2% ~ 32.6%)显著低于非转基因植株，说明BADH活性增加时，转基因植株的穿膜保护能力增强，将BADH基因导入白菜后，转基因植株的抗逆性得到了提高。同时，大多数转基因白菜在干旱、盐和聚乙二醇(PEG)胁迫下生长旺盛。转基因植株的平均长高率显著高于非转基因植株。结果表明，转BADH基因后，转基因白菜的耐旱、耐盐性状得到改善，可作为白菜抗逆性育种的基础种质。
{"title":"Agrobacterium tumefaciens-mediated transformation of cabbage with betaine aldehyde dehydrogenase gene.","authors":"Zhou Guoyan, Yang Zhengan, Zhang Yinhua, Guo Fenggen, Zhou Xiaogang, Zhang Shaosong, Sun Mao-lin, Wu Shaoyun, Ding Yuemei","doi":"10.3724/SP.J.1143.2009.09013","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09013","url":null,"abstract":"Cabbage(Brassica oleracea var.capitata) is one of the most popular and widely cultivated vegetable crops in the world. In this paper,BADH(betaine aldehyde dehydrogenase) gene derived from spinach was transformed into the genome of cabbage line 03079 mediated by Agrobacterium tumefaciens. To establish the ideal transformation platform,the main factors which affect the transformation efficiency were optimized through the orthogonal design of L9(34) ,including the type of infection medium,concentration of acetosyringone,the period of infection time and co-culture time. The results indicated that No. 9 was the best treatment combination,i.e,AA liquid medium was the optimal infection medium for the cabbage transformation,and co-culturing for 2 days after infection(20 min) would be favorable for the transformation. If supplemented with 200 μmol L-1 acetosyringone in infection medium,the transformation efficiency would be improved and the transformed plant regenaration ratio reached at 54.26%. Based on this optimal transformtion system,we obtained many transgenic plants,and PCR analysis using BADH gene primers and Southern blot analysis indicated that the BADH gene had been integrated into genome of cabbage. The BADH enzymes activity of transgenic plants were tested after treated with NaCl,drought-tolerance and PEG stress. The results showed that the average values of the activity of BADH enzymes,which varied from 2.1 U to 3.6 U per mg in transgenic plants,were 1 to 3 times higher than that of un-transgenic plants,and there was a significant difference between transgenic and un-transgenic plants using Duncan′s Multiple Range Test. Furthermore,the average value of relative electronic conductivity(varying from 16.2% to 32.6%) of transgenic plants were significantly lower than that of un-transgenic plants,which indicated that the protection ability of membrane penetration was enhanced when BADH activity increasing and the stress resistance of the transgenic plants was improved by introduction BADH gene into cabbage. At the same time,most of the transgenic cabbage plants were vigrously growing under drought 、salt and polyethylene glycol(PEG) stresses. The average height increasing percentage of transgenic plants were significantly higher than that of un-transgenic plants. Those results showed that the traits of drought or salt tolerance of transgenic cabbage plants were improved after transformed with BADH gene,and our transgenic plants would be used as fundamental germplasm for stress-tolerant breeding of cabbage.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"150 1","pages":"335-343"},"PeriodicalIF":0.0,"publicationDate":"2010-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87982064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-30DOI: 10.3724/SP.J.1143.2009.09043
Yang Jun, Yang Yunqiang, Y. Yongping, Hu Xiangyang
We have established the extraction method of the total proteins of Antiaris toxicaria seed and the appropriate conditions for the two-dimensional eiectrophoresis(2-DE) of Antiaris toxicaria seed proteome. Through integrating and optimizing various conditions,we also established the 2-DE system including the sample preparation method with Tris-HCL based on TCA-actone,and the first-dimensional electrophoresis with immobilized pH gradients isoelectric focusing and the second dimensional electrophoresis with vertical electrophoresis of SDS-PAGE. We have gained a good 2-DE map in the analysis of sample preparation、sample resolution、isoelectric focusing(IEF) 、SDS-PAGE and gel staining. In order to explore the extraction method of proteome and technique of 2-DE,we compared the extraction method between the TCA-actone and the Tris-HCl,and improved the key steps of 2-DE. The Tris-HCl extraction was the most appropriate for Antiaris toxicaria seed proteome analysis because of the highest resolution and more informative 2-DE map. Those were the solid basis for the later research of studying on the discrepancy expression proteomics of Antiaris toxicaria Seed.
{"title":"The Sample Preparation and the Improvement Method of Two-Dimensional Electrophoresis of Proteins from Antiaris toxicaria (Moraceae) Seed","authors":"Yang Jun, Yang Yunqiang, Y. Yongping, Hu Xiangyang","doi":"10.3724/SP.J.1143.2009.09043","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09043","url":null,"abstract":"We have established the extraction method of the total proteins of Antiaris toxicaria seed and the appropriate conditions for the two-dimensional eiectrophoresis(2-DE) of Antiaris toxicaria seed proteome. Through integrating and optimizing various conditions,we also established the 2-DE system including the sample preparation method with Tris-HCL based on TCA-actone,and the first-dimensional electrophoresis with immobilized pH gradients isoelectric focusing and the second dimensional electrophoresis with vertical electrophoresis of SDS-PAGE. We have gained a good 2-DE map in the analysis of sample preparation、sample resolution、isoelectric focusing(IEF) 、SDS-PAGE and gel staining. In order to explore the extraction method of proteome and technique of 2-DE,we compared the extraction method between the TCA-actone and the Tris-HCl,and improved the key steps of 2-DE. The Tris-HCl extraction was the most appropriate for Antiaris toxicaria seed proteome analysis because of the highest resolution and more informative 2-DE map. Those were the solid basis for the later research of studying on the discrepancy expression proteomics of Antiaris toxicaria Seed.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"41 1","pages":"357-362"},"PeriodicalIF":0.0,"publicationDate":"2010-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85844060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-30DOI: 10.3724/SP.J.1143.2009.09066
Li Jing, Z. Wenhua
{"title":"PLDα1 modulated ABA-mediated main root elongation and regulated tip growth of root hair in Arabidopsis.","authors":"Li Jing, Z. Wenhua","doi":"10.3724/SP.J.1143.2009.09066","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09066","url":null,"abstract":"","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"50 1","pages":"309-316"},"PeriodicalIF":0.0,"publicationDate":"2010-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73767540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2010-03-30DOI: 10.3724/SP.J.1143.2009.09062
R. Liu, Hsieh Kun-Lung, Jikai Liu
A new guaiane-type sesquiterpene lactone, named 3β, 8α, 11α, 13-tetrahydroxy-10 (14)-guaien-1α, 4β, 5α, 6βH-6α, 12-olide (1), together with a known sesquiterpene lactone, cynarinin A (2), were isolated from the leaves of Cynara scolymus (Artichoke). The structure of 1 was elucidated on the basis of MS, IR, 1D, and 2D NMR.
{"title":"A New Sesquiterpene Lactone from the Leaves of Cynara scolymus (Compositae)","authors":"R. Liu, Hsieh Kun-Lung, Jikai Liu","doi":"10.3724/SP.J.1143.2009.09062","DOIUrl":"https://doi.org/10.3724/SP.J.1143.2009.09062","url":null,"abstract":"A new guaiane-type sesquiterpene lactone, named 3β, 8α, 11α, 13-tetrahydroxy-10 (14)-guaien-1α, 4β, 5α, 6βH-6α, 12-olide (1), together with a known sesquiterpene lactone, cynarinin A (2), were isolated from the leaves of Cynara scolymus (Artichoke). The structure of 1 was elucidated on the basis of MS, IR, 1D, and 2D NMR.","PeriodicalId":7106,"journal":{"name":"Acta Botanica Yunnanica","volume":"23 1","pages":"383-385"},"PeriodicalIF":0.0,"publicationDate":"2010-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74416971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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