Organic volatile chemicals such as amines are often not completely removed by practical manufacturing techniques and consequently their low levels are present in most pharmaceuticals. Based on good manufacturing practices and for the safety of the patients the level of hazardous for human health amines in pharmaceuticals have to be strictly controlled. Moreover, analysis of volatile amines in drug product has become recently an emerging topic of interest for analytical chemists since such residual impurities as: secondary amines, tertiary amine and other amine derivatives (in certain condition) have been reported to be a precursors of carcinogenic N-nitrosamines (NAs). In this study we present five new methods for control of such amines as: tert-butylamine (TBA), pyrrolidine, 4-fluoroaniline (4-FA), diisopropyl-ethylamine (DIPEA) and tetrabutylammonium hydrogensulfate (TBAHS) in pharmaceutical active ingredients (APIs) using GC-MS and LC-MS techniques. The validations results clearly demonstrate that the analytical procedures are suitable for their intended purpose. All revealed validations results meet the requirements of the ICH Q2R1 validation guidelines and the Q3A R2 guideline for residual impurities.
{"title":"Application of GC-MS and LC-MS techniques for direct analysis of amines in pharmaceutical substances","authors":"A. Witkowska, E. Stolarczyk, A. Groman, M. Zezula","doi":"10.32383/appdr/156083","DOIUrl":"https://doi.org/10.32383/appdr/156083","url":null,"abstract":"Organic volatile chemicals such as amines are often not completely removed by practical manufacturing techniques and consequently their low levels are present in most pharmaceuticals. Based on good manufacturing practices and for the safety of the patients the level of hazardous for human health amines in pharmaceuticals have to be strictly controlled. Moreover, analysis of volatile amines in drug product has become recently an emerging topic of interest for analytical chemists since such residual impurities as: secondary amines, tertiary amine and other amine derivatives (in certain condition) have been reported to be a precursors of carcinogenic N-nitrosamines (NAs). In this study we present five new methods for control of such amines as: tert-butylamine (TBA), pyrrolidine, 4-fluoroaniline (4-FA), diisopropyl-ethylamine (DIPEA) and tetrabutylammonium hydrogensulfate (TBAHS) in pharmaceutical active ingredients (APIs) using GC-MS and LC-MS techniques. The validations results clearly demonstrate that the analytical procedures are suitable for their intended purpose. All revealed validations results meet the requirements of the ICH Q2R1 validation guidelines and the Q3A R2 guideline for residual impurities.","PeriodicalId":7147,"journal":{"name":"Acta poloniae pharmaceutica","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46592392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diabetes is closely relevant to muscle dysfunction. Chlorogenic acid (CGA) possesses various therapeutic functions in diabetic complications. However, no research implied the amelioration of CGA on diabetes-associated muscular atrophy. In this study, diabetic model was established by streptozotocin (STZ) to appraise roles of CGA (30 mg/kg/d for 8 weeks) on diabetes-associated muscular atrophy. Our researches demonstrated that CGA enhanced representative myocyte cross-sections, gastrocnemius weight and grip strength. In serum, CGA restricted CK, LDH, MDA, TNF-a and IL-6 levels, while elevated T-ACO, SOD and CAT levels against diabetic-evoked muscle damage. In skeletal muscle, CGA increased BCL-2 expression, while reduced FBXO-32, MURF-1, DDIT-3, GRP-87 and BAX expressions to improve diabetic muscular atrophy. In conclusion, CGA protected from diabetic-associated muscular atrophy through its bioactivities on the regulation of endoplasmic reticulum stress and apoptosis, and could be a powerful remedy approach in the ameliorations of diabetic muscle atrophy.
{"title":"Chlorogenic acid improves diabetes-associated muscular atrophy in mice","authors":"Xianchu Liu, Ming-xue Liu","doi":"10.32383/appdr/158786","DOIUrl":"https://doi.org/10.32383/appdr/158786","url":null,"abstract":"Diabetes is closely relevant to muscle dysfunction. Chlorogenic acid (CGA) possesses various therapeutic functions in diabetic complications. However, no research implied the amelioration of CGA on diabetes-associated muscular atrophy. In this study, diabetic model was established by streptozotocin (STZ) to appraise roles of CGA (30 mg/kg/d for 8 weeks) on diabetes-associated muscular atrophy. Our researches demonstrated that CGA enhanced representative myocyte cross-sections, gastrocnemius weight and grip strength. In serum, CGA restricted CK, LDH, MDA, TNF-a and IL-6 levels, while elevated T-ACO, SOD and CAT levels against diabetic-evoked muscle damage. In skeletal muscle, CGA increased BCL-2 expression, while reduced FBXO-32, MURF-1, DDIT-3, GRP-87 and BAX expressions to improve diabetic muscular atrophy. In conclusion, CGA protected from diabetic-associated muscular atrophy through its bioactivities on the regulation of endoplasmic reticulum stress and apoptosis, and could be a powerful remedy approach in the ameliorations of diabetic muscle atrophy.","PeriodicalId":7147,"journal":{"name":"Acta poloniae pharmaceutica","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49373168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paulina Adamczyk, M. Lemieszek, Ewa Ozimek, M. Pleszczyńska, M. Locatelli, M. Siwulski, A. Sroka-Bartnicka, A. Wiater
One of the important features of prebiotics is their beneficial effect on the health of the host, which consists in the inhibition of the growth of harmful bacteria, including strains causing caries. The aim of the present study was to evaluate the cariogenic potential of a hydrolyzate of (1→3)-α-D-glucan (GOS) isolated from fruiting bodies of Laetiporus sulphureus, consisting of (1→3)-α-D-glucooligosaccharides (85.6%) and glucose (14.4%). The study was conducted in cultures of cariogenic bacteria belonging to the genus Streptococcus (S. mutans CAPM 6067, S. sobrinus DSMZ 20381, S. sobrinus/downei CCUG 21020, and S. sanguis ATCC 10556). The investigation of the cariogenicity of GOS included examination of the influence of the preparation on the growth of the above mentioned bacteria, their ability to synthesize mutan, and the formation of a sucrose-dependent biofilm. GOS significantly inhibited the growth of the investigated streptococci, and the observed beneficial effect was stronger than the changes induced by a commercial prebiotic preparation containing a mixture of fructooligosaccharides (FOS). Furthermore, GOS inhibited the mutan synthesis by the above mentioned bacteria but did not exert an impact on the formation of artificial dental plaque. In conclusion, the studies conducted showed low cariogenicity of GOS and confirmed the safety of its use as a dietary supplement.
益生元的一个重要特征是它们对宿主健康的有益作用，这包括抑制有害细菌的生长，包括引起龋齿的菌株。摘要本研究旨在评价从硫酸Laetiporus suureus子实体中分离得到的(1→3)-α- d -葡聚糖(GOS)水解产物(1→3)-α- d -葡聚糖(GOS)(85.6%)和葡萄糖(14.4%)的致龋性。该研究是在链球菌属的蛀牙细菌(S. mutans CAPM 6067, S. sobrinus DSMZ 20381, S. sobrinus/downei CCUG 21020和S. sanguis ATCC 10556)中进行的。GOS致癌性的研究包括考察GOS制剂对上述细菌生长的影响、对其合成诱变物的能力的影响以及对蔗糖依赖性生物膜形成的影响。GOS显著抑制了所研究链球菌的生长，并且所观察到的有益效果强于含有低聚果糖(FOS)混合物的商业益生元制剂所引起的变化。此外，GOS抑制了上述细菌的突变体合成，但对人工牙菌斑的形成没有影响。综上所述，研究表明GOS的致癌性低，证实了其作为膳食补充剂的安全性。
{"title":"EVALUATION OF THE CARIOGENICITY OF PREBIOTIC (1→3)-α-D-GLUCOOLIGOSACCHARIDES","authors":"Paulina Adamczyk, M. Lemieszek, Ewa Ozimek, M. Pleszczyńska, M. Locatelli, M. Siwulski, A. Sroka-Bartnicka, A. Wiater","doi":"10.32383/appdr/158785","DOIUrl":"https://doi.org/10.32383/appdr/158785","url":null,"abstract":"One of the important features of prebiotics is their beneficial effect on the health of the host, which consists in the inhibition of the growth of harmful bacteria, including strains causing caries. The aim of the present study was to evaluate the cariogenic potential of a hydrolyzate of (1→3)-α-D-glucan (GOS) isolated from fruiting bodies of Laetiporus sulphureus, consisting of (1→3)-α-D-glucooligosaccharides (85.6%) and glucose (14.4%). The study was conducted in cultures of cariogenic bacteria belonging to the genus Streptococcus (S. mutans CAPM 6067, S. sobrinus DSMZ 20381, S. sobrinus/downei CCUG 21020, and S. sanguis ATCC 10556). The investigation of the cariogenicity of GOS included examination of the influence of the preparation on the growth of the above mentioned bacteria, their ability to synthesize mutan, and the formation of a sucrose-dependent biofilm. GOS significantly inhibited the growth of the investigated streptococci, and the observed beneficial effect was stronger than the changes induced by a commercial prebiotic preparation containing a mixture of fructooligosaccharides (FOS). Furthermore, GOS inhibited the mutan synthesis by the above mentioned bacteria but did not exert an impact on the formation of artificial dental plaque. In conclusion, the studies conducted showed low cariogenicity of GOS and confirmed the safety of its use as a dietary supplement.","PeriodicalId":7147,"journal":{"name":"Acta poloniae pharmaceutica","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44854344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Ekinci, B. Suleyman, R. Mammadov, A. Gezer, A. Mendil, Nergis Akbaş, S. Bulut, Ç. Dal, H. Suleyman
Diabetic neuropathies are the most frequent complication of diabetes. While numerous metabolic pathways are disrupted in diabetic neuropathy, oxidative stress has been indicated as a significant reason for this condition. In this study, the effect of carvacrol, which has antioxidant effects, on experimental diabetic neuropathy and neuropathic pain was investigated. Alloxan was used to induce diabetes in the experiment. Diabetes was created by administering 120mg/kg of alloxan intraperitoneally (i.p) once a day for 3 days. Rats with a blood glucose concentration above 250mg/kg in the blood taken from the tail veins at the end of three days were considered diabetic. Rats were categorized under healthy control (HG), alloxan-induced hyperglycemia (AG), and alloxan-induced hyperglycemia + carvacrol-treated (ACG) groups. Carvacrol was i.p injected at 50 mg/kg dose to the ACG (n=6) group of rats with hyperglycemia. The same volume of distilled water as the solvent was applied in the same way to AG (n=6) and HG (n=6) rat groups. This procedure was repeated once a day for three months.Carvacrol showed anti-hyperglycemic effect in diabetic rats, protective effect against lowering pain threshold and analgesic activity in rat paws in rats. Carvacrol prevented the oxidant/antioxidant balance from changing in favor of oxidants. The results supported that carvacrol is an agent against alloxan-induced peripheral diabetic neuropathic pain.
{"title":"The effect of carvacrol upon experımentally ınduced dıabetıc neuropathy and neuropathıc paın ın rats","authors":"B. Ekinci, B. Suleyman, R. Mammadov, A. Gezer, A. Mendil, Nergis Akbaş, S. Bulut, Ç. Dal, H. Suleyman","doi":"10.32383/appdr/155354","DOIUrl":"https://doi.org/10.32383/appdr/155354","url":null,"abstract":"Diabetic neuropathies are the most frequent complication of diabetes. While numerous metabolic pathways are disrupted in diabetic neuropathy, oxidative stress has been indicated as a significant reason for this condition. In this study, the effect of carvacrol, which has antioxidant effects, on experimental diabetic neuropathy and neuropathic pain was investigated. Alloxan was used to induce diabetes in the experiment. Diabetes was created by administering 120mg/kg of alloxan intraperitoneally (i.p) once a day for 3 days. Rats with a blood glucose concentration above 250mg/kg in the blood taken from the tail veins at the end of three days were considered diabetic. Rats were categorized under healthy control (HG), alloxan-induced hyperglycemia (AG), and alloxan-induced hyperglycemia + carvacrol-treated (ACG) groups. Carvacrol was i.p injected at 50 mg/kg dose to the ACG (n=6) group of rats with hyperglycemia. The same volume of distilled water as the solvent was applied in the same way to AG (n=6) and HG (n=6) rat groups. This procedure was repeated once a day for three months.Carvacrol showed anti-hyperglycemic effect in diabetic rats, protective effect against lowering pain threshold and analgesic activity in rat paws in rats. Carvacrol prevented the oxidant/antioxidant balance from changing in favor of oxidants. The results supported that carvacrol is an agent against alloxan-induced peripheral diabetic neuropathic pain.","PeriodicalId":7147,"journal":{"name":"Acta poloniae pharmaceutica","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44381827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Katarzyna Bober-Majnusz, E. Bębenek, E. Chrobak, Monika Kadela-Tomanek
Chemometric analysis is often used in many fields of science. One of the most popular are cluster analysis and principal component analysis. The presented work considers those methods to evaluate the lipophilicity of groups of pentacyclic triterpene derivatives. They are newly synthesized compounds with biological activity, so they can be used in the future as drugs and lipophilicity is an essential parameter. The experimental values of lipophilicity were determined using thin-layer chromatography. The plates were precoated with silica gel, and a mixture of 1,4-dioxane and acetate buffer was applied as the mobile phase. The experimental values of lipophilicity for investigated compounds were correlated with lipophilicity taken from freely available databases (ALOGPs, KowWin, XLOGP2, XLOGP3, miLogP, AClogP, ALOGP, MLOGP, iLOGP, WLOGP and SILICOS-IT). Those experimental values were also correlated with physicochemical properties such as molecular weight, the topological polar surface of the molecule, the number of rotatable bonds, and the number of donor and acceptor sites for hydrogen bonds. Some correlation equations could be formed for the correlation obtained. The cluster and principal component analysis were done based on the data obtained. All the experimental lipophilicity data correlate well with those theoretically calculated based on the structural formula. Almost all dependencies can be described by the correlation equation, with a high correlation coefficient. Thus, it is possible to infer the lipophilicity values of triterpene derivatives without any laboratory work. The physicochemical properties turned out to be less valuable and, apart from the compounds' molar mass, not helpful.
{"title":"Application of chemometric methods for determination the lipophilicity of pentacyclic triterpene derivatives","authors":"Katarzyna Bober-Majnusz, E. Bębenek, E. Chrobak, Monika Kadela-Tomanek","doi":"10.32383/appdr/156084","DOIUrl":"https://doi.org/10.32383/appdr/156084","url":null,"abstract":"Chemometric analysis is often used in many fields of science. One of the most popular are cluster analysis and principal component analysis. The presented work considers those methods to evaluate the lipophilicity of groups of pentacyclic triterpene derivatives. They are newly synthesized compounds with biological activity, so they can be used in the future as drugs and lipophilicity is an essential parameter. The experimental values of lipophilicity were determined using thin-layer chromatography. The plates were precoated with silica gel, and a mixture of 1,4-dioxane and acetate buffer was applied as the mobile phase. The experimental values of lipophilicity for investigated compounds were correlated with lipophilicity taken from freely available databases (ALOGPs, KowWin, XLOGP2, XLOGP3, miLogP, AClogP, ALOGP, MLOGP, iLOGP, WLOGP and SILICOS-IT). Those experimental values were also correlated with physicochemical properties such as molecular weight, the topological polar surface of the molecule, the number of rotatable bonds, and the number of donor and acceptor sites for hydrogen bonds. Some correlation equations could be formed for the correlation obtained. The cluster and principal component analysis were done based on the data obtained. All the experimental lipophilicity data correlate well with those theoretically calculated based on the structural formula. Almost all dependencies can be described by the correlation equation, with a high correlation coefficient. Thus, it is possible to infer the lipophilicity values of triterpene derivatives without any laboratory work. The physicochemical properties turned out to be less valuable and, apart from the compounds' molar mass, not helpful.","PeriodicalId":7147,"journal":{"name":"Acta poloniae pharmaceutica","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46456284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jana Desnica, M. Kostić, S. Jankovic, Sara Mijailovic, K. Parezanović Ilić, O. Milovanović, D. Vulovic
Background/purpose: The incidence of facial fractures has a tendency to rise, which positively correlates with increasing total costs of treating this medical condition. The aim of this study was to identify the total costs of the hospital treatment of facial fractures and to analyze its main determinants. Materials and methods: This retrospective "cost-of-illness" study "from bottom to the top" approach was performed from the perspective of the Republic Health Insurance Fund. This study included 46 patients who were treated due to facial fractures at the Clinic for Maxillofacial Surgery at the Clinical Center Kragujevac in the period from the beginning of December 2017. to January 31, 2019. Results: The total costs of hospital treatment of all types of treated fractures amounted to 20,214.30€, and the average total cost per patient was estimated at 439.44±299.53€. The costs related to the length of hospitalization represented the largest part of the total direct costs, with a contribution of 31% and with a value of 6,329.30±80.18 €. Conclusions: Results of this pilot study pointed out that the total costs of treating facial fractures in the socio-economic sphere of Balkan countries are lower than in other health care systems, mostly due to differences in valuing medical services compared to countries within the European Union. Due to the rising incidence of injuries and obligatory hospital treatment of these conditions, this kind of pharmacoeconomic evaluation could contribute to the introduction of new therapeutic strategies for adequate allocation of resources within health systems.
{"title":"Pharmacoeconomic aspects of hospital treatment of facial fractures - cost of illness study based on data from Balkan country with recent history of social and economic transition","authors":"Jana Desnica, M. Kostić, S. Jankovic, Sara Mijailovic, K. Parezanović Ilić, O. Milovanović, D. Vulovic","doi":"10.32383/appdr/157009","DOIUrl":"https://doi.org/10.32383/appdr/157009","url":null,"abstract":"Background/purpose: The incidence of facial fractures has a tendency to rise, which positively correlates with increasing total costs of treating this medical condition. The aim of this study was to identify the total costs of the hospital treatment of facial fractures and to analyze its main determinants.\u0000Materials and methods: This retrospective \"cost-of-illness\" study \"from bottom to the top\" approach was performed from the perspective of the Republic Health Insurance Fund. This study included 46 patients who were treated due to facial fractures at the Clinic for Maxillofacial Surgery at the Clinical Center Kragujevac in the period from the beginning of December 2017. to January 31, 2019.\u0000Results: The total costs of hospital treatment of all types of treated fractures amounted to 20,214.30€, and the average total cost per patient was estimated at 439.44±299.53€. The costs related to the length of hospitalization represented the largest part of the total direct costs, with a contribution of 31% and with a value of 6,329.30±80.18 €. \u0000Conclusions: Results of this pilot study pointed out that the total costs of treating facial fractures in the socio-economic sphere of Balkan countries are lower than in other health care systems, mostly due to differences in valuing medical services compared to countries within the European Union. Due to the rising incidence of injuries and obligatory hospital treatment of these conditions, this kind of pharmacoeconomic evaluation could contribute to the introduction of new therapeutic strategies for adequate allocation of resources within health systems.","PeriodicalId":7147,"journal":{"name":"Acta poloniae pharmaceutica","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47512222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Marinković, Sladjana V. Trpkovic, Ana D. Sekulic, A. Ilic, A. Pavlovic, Barbara Loboda, V. Grbovic, Ž. Todorovic, S. Simovic, N. Zdravković
Acute pancreatitis (AP) is an inflammatory disease of the pancreas that causes local damage and systemic inflammatory response. Some of the numerical scoring systems used in the intensive care unit for the assessment of critically ill patients such as APACHE II and MEWS score could be used for AP, beside the scoring systems specific to AP (Ranson score, Pancreas score, BISAP). Therefore, the aim of this study was to examine the significance of inflammatory biomarkers and scoring systems in the evaluation of the severity of AP and outcomes. The study was conducted as a cohort prospective study, examining patients with AP, of both sexes. Laboratory analyses, as well as the scoring systems: Ranson, Pancreas score, Bedside Index of Severity in Acute Pancreatitis (BISAP), and Acute Physiology and Chronic Health Evaluation II (APACHE II) were collected on day zero and 48h after admission. The study included 50 patients of whom 8 died. The most reliable score for predicting AP severity was APACHE II0 and 48AUROC (0.753; 0.768) in relation to the inflammatory biomarkers. For initial prediction of the treatment outcome, APACHE II0, BISAP0, and CRP0 AUROC (0.813; 0.807; 0.753) had the highest discrimination rates and after 48h, APACHE II48, Ranson48, BISAP48, and Pancreas48 AUROC (0.917; 0.856; 0.789; 0.729). There was a statistically significant correlation between CRP0 and BISAP0 (p=0.006) and between PCT and all day-zero scores. All tested screening systems showed reliability in predicting AP severity and treatment outcomes. The best predictive power was demonstrated by the APACHE II score.
{"title":"The importance of using inflammatory biomarkers and scoring systems in early assessment of severity and outcome of acute pancreatitis treatment","authors":"O. Marinković, Sladjana V. Trpkovic, Ana D. Sekulic, A. Ilic, A. Pavlovic, Barbara Loboda, V. Grbovic, Ž. Todorovic, S. Simovic, N. Zdravković","doi":"10.32383/appdr/158169","DOIUrl":"https://doi.org/10.32383/appdr/158169","url":null,"abstract":"Acute pancreatitis (AP) is an inflammatory disease of the pancreas that causes local damage and systemic inflammatory response. Some of the numerical scoring systems used in the intensive care unit for the assessment of critically ill patients such as APACHE II and MEWS score could be used for AP, beside the scoring systems specific to AP (Ranson score, Pancreas score, BISAP). Therefore, the aim of this study was to examine the significance of inflammatory biomarkers and scoring systems in the evaluation of the severity of AP and outcomes.\u0000The study was conducted as a cohort prospective study, examining patients with AP, of both sexes. Laboratory analyses, as well as the scoring systems: Ranson, Pancreas score, Bedside Index of Severity in Acute Pancreatitis (BISAP), and Acute Physiology and Chronic Health Evaluation II (APACHE II) were collected on day zero and 48h after admission.\u0000The study included 50 patients of whom 8 died. The most reliable score for predicting AP severity was APACHE II0 and 48AUROC (0.753; 0.768) in relation to the inflammatory biomarkers. For initial prediction of the treatment outcome, APACHE II0, BISAP0, and CRP0 AUROC (0.813; 0.807; 0.753) had the highest discrimination rates and after 48h, APACHE II48, Ranson48, BISAP48, and Pancreas48 AUROC (0.917; 0.856; 0.789; 0.729). There was a statistically significant correlation between CRP0 and BISAP0 (p=0.006) and between PCT and all day-zero scores.\u0000All tested screening systems showed reliability in predicting AP severity and treatment outcomes. The best predictive power was demonstrated by the APACHE II score.","PeriodicalId":7147,"journal":{"name":"Acta poloniae pharmaceutica","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43663950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The problem of drug-food interactions is of particular concern to the elderly, as polypharmacy is most prevalent in this population. The study aimed to assess the awareness and knowledge of drug-food interactions among Polish third-age university listeners before and after training on that topic. A total of 143 participants, with a mean age of 71.5 ± 5.5 years, took part in the study. 123 participants (86%) completed the pre- and post-training questionnaires. 91% of respondents had heard that food can affect the effectiveness of drugs and a similar number stated that they are aware of what time to take their medications. Information about drug-food interactions was most frequently obtained from the physician (34%), the drug leaflet (30%), or the internet article (23%). The mean percentage of correct answers to questions testing knowledge of drug-food interactions before and after training was 32% and 57%, respectively (p < 0.05). To determine the structure of relationships between parameters (or categories of parameters), the results were examined using correspondence analysis (CA). The categories of parameters with the highest quality of representation in the CA model were indicated and the parameters characterized by the highest strength of coexistence were determined. The majority of study participants were aware of drug-food interactions, but their knowledge of the topic was poor, and often incorrect. The training significantly increased knowledge of drug-food interactions. The study indicates the need to educate geriatric patients on the correct use of drugs with food and on the importance of drug-food interactions.
{"title":"Awareness and knowledge of drug-food interactions among polish third-age university listeners before and after training – a questionnaire study","authors":"Agnieszka Wiesner, P. Zagrodzki, P. Paśko","doi":"10.32383/appdr/156248","DOIUrl":"https://doi.org/10.32383/appdr/156248","url":null,"abstract":"The problem of drug-food interactions is of particular concern to the elderly, as polypharmacy is most prevalent in this population. The study aimed to assess the awareness and knowledge of drug-food interactions among Polish third-age university listeners before and after training on that topic. A total of 143 participants, with a mean age of 71.5 ± 5.5 years, took part in the study. 123 participants (86%) completed the pre- and post-training questionnaires. 91% of respondents had heard that food can affect the effectiveness of drugs and a similar number stated that they are aware of what time to take their medications. Information about drug-food interactions was most frequently obtained from the physician (34%), the drug leaflet (30%), or the internet article (23%). The mean percentage of correct answers to questions testing knowledge of drug-food interactions before and after training was 32% and 57%, respectively (p < 0.05). To determine the structure of relationships between parameters (or categories of parameters), the results were examined using correspondence analysis (CA). The categories of parameters with the highest quality of representation in the CA model were indicated and the parameters characterized by the highest strength of coexistence were determined. The majority of study participants were aware of drug-food interactions, but their knowledge of the topic was poor, and often incorrect. The training significantly increased knowledge of drug-food interactions. The study indicates the need to educate geriatric patients on the correct use of drugs with food and on the importance of drug-food interactions.","PeriodicalId":7147,"journal":{"name":"Acta poloniae pharmaceutica","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42768485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zefine Uğraş, F. Tok, E. Şalva, G. Ultav, Bedia Kaymakcıoğlu-Koçyiğit
In this study, a series of novel 2-pyrazoline derivatives were synthesized and their structures were established by using spectral methods. The antiproliferative activities of compounds were investigated against human cell lines A-549 and MCF-7 by MTT assay and L-929 (mouse normal fibroblast) cell cytotoxicity was also examined. Apoptotic effects of the compounds in breast and lung cancer cells were assessed by Annexin V-FITC apoptosis assay using flow cytometry. The antiproliferative effect on lung carcinoma of the synthesized compounds was higher than breast carcinoma. Moreover, it was observed that none of all synthesized compounds have cytotoxic activity in healthy cells. Flow cytometry studies have shown that compounds induced apoptosis at high concentration. Additionally, fluorescence cell imaging studies were performed for the first time in A-549 and MCF-7 cancer cell lines to determine the potential of the biosensor compounds by fluorescence microscopy. Compounds 4b, 4d, 4e and 4f showed fluorescence properties by considering microscopic imaging.
{"title":"Synthesis and structural characterization of novel 2-pyrazoline derivatives: Evaluation of their antiproliferative activity and fluorescence properties","authors":"Zefine Uğraş, F. Tok, E. Şalva, G. Ultav, Bedia Kaymakcıoğlu-Koçyiğit","doi":"10.32383/appdr/157503","DOIUrl":"https://doi.org/10.32383/appdr/157503","url":null,"abstract":"In this study, a series of novel 2-pyrazoline derivatives were synthesized and their structures were established by using spectral methods. The antiproliferative activities of compounds were investigated against human cell lines A-549 and MCF-7 by MTT assay and L-929 (mouse normal fibroblast) cell cytotoxicity was also examined. Apoptotic effects of the compounds in breast and lung cancer cells were assessed by Annexin V-FITC apoptosis assay using flow cytometry. The antiproliferative effect on lung carcinoma of the synthesized compounds was higher than breast carcinoma. Moreover, it was observed that none of all synthesized compounds have cytotoxic activity in healthy cells. Flow cytometry studies have shown that compounds induced apoptosis at high concentration. Additionally, fluorescence cell imaging studies were performed for the first time in A-549 and MCF-7 cancer cell lines to determine the potential of the biosensor compounds by fluorescence microscopy. Compounds 4b, 4d, 4e and 4f showed fluorescence properties by considering microscopic imaging.","PeriodicalId":7147,"journal":{"name":"Acta poloniae pharmaceutica","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47905663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Gulbicka, Dagmara Pietkiewicz, Eliza Matuszewska, Szymon Plewa, Joanna Matysiak, M. Grzymisławski, Z. Kokot
The malabsorption syndrome includes many clinical units that lead to chronic diarrhea and malnutrition. In celiac disease and small intestine bacterial overgrowth making the diagnosis is not always straightforward, and the diagnosis often requires combining blood antibody tests, intestinal biopsies, and specific genetic testing. The study aimed to assess patients' protein/peptide profiles with celiac disease (n=31), small intestinal bacterial overgrowth (n=28), and the control group (n=30). Attempts have also been made to identify potential new diagnostic markers. After appropriate preparation, a proteomic analysis of peptides was performed using the MALDI–TOF mass spectrometer. Identified proteins were fibrinogen alpha-chain, kininogen-1, mucin 3A, complement C3, complement C4A, and inter-alpha-trypsin inhibitor heavy chain H4. Performed analyses indicated some proteins that could be potentially involved in the presence of the disease in question and proved that proteomic profiling might serve as a powerful diagnostic tool. Due to the difficulties in celiac disease and small intestine bacterial overgrowth diagnosis, there is a clear need for further investigation of the biological role of proteins potentially involved in the course of the disease.
{"title":"Evaluation of the proteomic profile in patients with celiac disease and malabsorption syndrome","authors":"P. Gulbicka, Dagmara Pietkiewicz, Eliza Matuszewska, Szymon Plewa, Joanna Matysiak, M. Grzymisławski, Z. Kokot","doi":"10.32383/appdr/157010","DOIUrl":"https://doi.org/10.32383/appdr/157010","url":null,"abstract":"The malabsorption syndrome includes many clinical units that lead to chronic diarrhea and malnutrition. In celiac disease and small intestine bacterial overgrowth making the diagnosis is not always straightforward, and the diagnosis often requires combining blood antibody tests, intestinal biopsies, and specific genetic testing. The study aimed to assess patients' protein/peptide profiles with celiac disease (n=31), small intestinal bacterial overgrowth (n=28), and the control group (n=30). Attempts have also been made to identify potential new diagnostic markers. After appropriate preparation, a proteomic analysis of peptides was performed using the MALDI–TOF mass spectrometer. Identified proteins were fibrinogen alpha-chain, kininogen-1, mucin 3A, complement C3, complement C4A, and inter-alpha-trypsin inhibitor heavy chain H4. Performed analyses indicated some proteins that could be potentially involved in the presence of the disease in question and proved that proteomic profiling might serve as a powerful diagnostic tool. Due to the difficulties in celiac disease and small intestine bacterial overgrowth diagnosis, there is a clear need for further investigation of the biological role of proteins potentially involved in the course of the disease.","PeriodicalId":7147,"journal":{"name":"Acta poloniae pharmaceutica","volume":" ","pages":""},"PeriodicalIF":0.4,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42268259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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