Analysis & sensing最新文献

The cover picture represents selective visualization of hydrogen polysulfides (H₂S_n, n≥2) over other RSS, ROS and RNS by a fluorogenic reaction of 3′-O-(2-nitrobenzenesulfonyl)fluorescein, chosen as a practical H₂S_n probe from the candidate pool of 2- or 4-nitrobenzenesulfonylated fluorescein derivatives that were evaluated. This fluorogenic response occurred by straightforward one-step deprotection of the probe with H₂S_n in a selective and sensitive manner. Cover design by Yuka Shinohara. More information can be found in the Research Article by K. Tsukamoto, H. Maeda, and co-workers.

Invited for this month's cover is the group of Prof. Hatsuo Maeda at Hyogo Medical University, Japan. The cover picture features selective visualization of hydrogen polysulfides (H₂S_n, n ≥ 2) over other related species through a fluorogenic reaction of 3′-O-(2-nitrobenzenesulfonyl)fluorescein, that was screened as the best practical H₂S_n probe among 2- or 4-nitrobenzenesulfonylated fluorescein derivatives. More information can be found in the Research Article by K. Tsukamoto, H. Maeda, and co-workers.

Many DNA aptamers have been reported to target the spike protein (S-protein) of SARS-CoV-2. These aptamers display different affinities or recognize different epitopes of the S-protein. We conducted a comparative study of 9 DNA aptamers for binding to several variants of the S-protein and pseudoviruses using the same testing methods, including dot-blot assays and enzyme-linked aptamer binding assays, to evaluate their affinity ranking and analytical utility. Moreover, the binding sites of these aptamers on the S-protein were examined using aptamer competition assays to understand the effect of S-protein mutations on aptamer affinity and the degree of overlapping of the binding sites by these aptamers.

The cover feature image shows electrochemical sensing using single-atom materials (SAMs) featuring isolated metal sites embedded inside inorganic or organic supports. Thanks to the well-defined active sites, SAMs have demonstrated expectational performance. The current research progress of metal-inorganic/organic SAMs is discussed, providing an introductive outline of the rational sensor design toward higher sensitivity, selectivity, and stability. Cover design by Weiran Zheng. More information can be found in the Review by Weiran Zheng.

The cover feature image shows a cartoon representation of matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), which can produce in-source fragmentation of lipids and cause artificial annotations from biological samples, particularly for phosphatidic acids. Whether these annotations are endogenous or artificial may be assessed by comparison with nanospray desorption electrospray ionization mass spectrometry imaging (nano-DESI-MSI). More information can be found in the Research Article by Christopher Anderton and co-workers.

The cover picture shows a semiconductor-based biosensor with potential to detect the presence of a specific biomarker of human induced pluripotent stem cell (hiPSCs) in a culture medium. More information can be found in the Research Article by S. Hideshima, T. Osaka, and co-workers.

This Perspective provides a concise overview of the field of Luminescence Sensing, which enables highly sensitive, selective, and versatile measurements. The emergence of new luminescent nanomaterials and the prospective of deploying new luminescence-based detection schemes for solving real-world problems, e. g., in medical diagnosis, food safety, and environmental sciences, has led to a continuous growth of the field over recent years. The contributions to the Special Collection on Luminescence Sensing – with a focus on the versatility and advantages of lanthanide complexes for advanced biosensing – serve as an excellent foundation to highlight and discuss recent developments and challenges.

The rapid detection of proteins is very important in the early diagnosis of diseases. Gold nanoparticles (AuNPs) can be engineered to bind biomolecules efficiently and differentially. Cross-reactive sensor arrays have high sensitivity for sensing proteins using differential interactions between sensor elements and bioanalytes. A new sensor array was fabricated using surface-charged AuNPs with dyes supramolecularly encapsulated into the AuNP monolayer. The fluorescence of dyes is partially quenched by the AuNPs and can be restored or further quenched due to the differential interactions between AuNPs with proteins. This sensing system enables the discrimination of proteins in both buffer and human serum, providing a potential tool for real-world disease diagnostics.

Wearable bioelectronics is a promising next-generation technology for its versatility in personalized applications. Measuring lactate is one of the growing trends in wearable biosensing research. To achieve this goal, enzymes capable of catalyzing reactions involving lactate must be coupled with bioelectrode components, creating a variety of biodevices such as biosensors, biofuel cells, and other devices harvesting energy from wearers. This review provides a brief history of noninvasive and minimally invasive enzyme-based lactate biosensors and energy biodevices. We introduce key principles of lactate oxidase and lactate dehydrogenase, together with immobilization strategies for efficient electrical contacts between redox enzymes and electrode supports. Additionally, we discuss recent examples of advanced wearable enzymatic lactate sensors and elaborate on a collection of self-powered wearable energy biodevices (e. g., biofuel cells, triboelectric nanogenerators, and piezoelectric devices). Lastly, we finish this review with discussions on challenges in developing lactate bioelectronics and provide our outlook on the prospects and future directions of this compelling technology.

Differential sensing is a technique that uses an array of cross-reactive receptors to create a unique fingerprint to detect analytes. Over the past two decades significant progress in the field has highlighted the power of this approach, enabling detection with commercially available or synthetically simple sensors, discrimination of structurally similar and challenging analytes, and low concentration detection. In this Concept paper, we briefly review developments in the field and highlight areas for future exploration. We believe there is still much room to grow, particularly in designing biosensor arrays, achieving simultaneous quantification of multiple analytes (i. e., multiplexing), and implementation of more advanced machine learning algorithms in array response analysis.