Biomaterial investigations in dentistry最新文献

Purpose: High-translucent dental zirconia has been introduced as a suitable material for anterior monolithic restorations. The material composition differs from traditional 3Y-TZP both with regard to yttria content and grain size. Little is known regarding how these alterations affect other properties than translucency and flexural strength. The aim of this study was to evaluate the crack propagation resistance and hardness of dental zirconias with different yttria content and different manufacturing methods.

Materials and methods: Measurement of hardness (HV2/5) and crack propagation from the indents (damage tolerance) was performed using a hardness tester(Vicker) on a flat polished surface of five crowns from six different commercial dental zirconias; one hard-machined 3Y-TZP, three soft-machined 3-5% yttria-stabilized zirconias and two soft-machined zirconias with ≥5% yttria content.

Results: Damage control varied greatly among dental zirconias with different compositions and fabrication methods. The hard-machined 3Y-TZP had better crack propagation resistance than soft-machined, 3-5% yttria-stabilized zirconias.

Conslusion: The ultra-translucent zirconias with ≥5% yttria content had the lowest crack propagation resistance. Hardness is not a suitable indicator for damage tolerance.

Aim: This study aimed to assess the efficacy of cavity liners with/without the atmospheric cold plasma jet (ACPJ) for dentin remineralization.

Material and methods: The occlusal third of 24 extracted third molars was cut. An occlusal cavity was prepared in the dentin of each tooth with 1 mm depth and 2 mm diameter and demineralized with 37% phosphoric acid for 1 min. The teeth were randomly divided into 8 groups (n = 3). The first control group only underwent demineralization with phosphoric acid. The second control group underwent demineralization and helium plasma jet. Groups 3 to 5 were filled with calcium hydroxide (CH), RetroMTA (MTA) and Biodentine. Groups 6 to 8 were subjected to ACPJ, and all the groups were sealed with polycarboxylate. After 2 weeks of immersion in water, the teeth were longitudinally sectioned and their mineral content was analyzed using energy-dispersive X-ray spectroscopy (EDX).

Results: The interaction effect of ACPJ and type of liner was not significant (p > 0.05). Application of ACPJ in combination with liner had a significant effect on calcium and phosphorous contents (p < .05). The calcium and phosphorous contents in the control groups were significantly lower than other groups (p < .05). The calcium and phosphorous contents in the CH group were higher than the control but significantly lower than the MTA and Biodentine groups. The values in the MTA and Biodentine groups were the same and higher than other groups.

Conlusion: All three cavity liners significantly increased the calcium and phosphorous contents of dentin. This increase was significantly greater by the MTA and Biodentine and enhanced by the ACPJ.

Purpose: The purpose of this study was to assess fracture resistance, failure mode and stress concentration of a modified endocrown preparation design, under axial and lateral forces.

Materials and methods: Forty lower molars were divided into two groups (n = 20) and were restored with lithium disilicate glass-ceramic endocrowns following 2 preparation designs: Conventional, with circumferential butt margin 2 mm above the cemento-enamel junction; and Modified, by adding 2 grooves on the mesial side of the vestibular dentinal wall and on the distal side of the lingual dentinal wall. After cementation and thermomechanical cycling loading, half of the samples (n = 10) from each group were loaded axially and the other half (n = 10) was loaded laterally. Fracture resistance and failure modes were observed and the finite element analysis (FEA) was used to identify the stress concentration. Two-way ANOVA and Chi-square tests (α = 0.05) were used for in vitro data analyzes.

Results: Fracture resistance showed a statistically significant difference between conventional and modified preparations (p < .001), and between axial and lateral loadings (p < .001). Conventional preparation recorded 2914 N under axial loading and 1516 N under lateral loading, while modified preparation recorded 3329 N under axial loading and 1871 N under lateral loading. FEA showed that retention grooves have reduced the stress concentration under both loads for the tooth and the restoration.

Conclusion: Modified endocrown design showed higher fracture resistance than conventional endocrown. Lateral loading displayed a high percentage of severe fracture but under higher load to failure than the values reported for normal masticatory forces.

Aim: To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus (S. aureus).Methodology: The antimicrobial efficacy of the silver nanoparticles was determined by the standard methods of Clinical and Laboratory Standards Institute (CLSI). Different concentrations of silver nanoparticles were prepared, and MIC was calculated by tube macro-dilution method. The MBC was determined by the lowest concentration that kills 99.9% of the initial bacterial population. The data were analyzed by ANOVA and Tukey's post hoc test using SPSS software.

Results: The MIC and MBC of silver nanoparticles against S. aureus was found to be 0.625 mg/ml.

Conclusion: The result obtained from this study shows that silver nanoparticles have potential bactericidal effects against S. aureus at a concentration of 0.625 mg/ml. Silver nanoparticles can be incorporated in the root canal medicaments, sealers and irrigants as it possess potent antimicrobial efficacy against S. aureus.

Aims: The aims of this study were to present a novel method to analyse dentin bond strength and to evaluate the bond strength of combining adhesive systems and resin cement from different manufacturers.

Methods: Human wisdom teeth were ground flat to the dentin on parallel surfaces and axially cut into two parts. Dentin cylinders (Ø 3 mm) were drilled from one half of each tooth. The other half from each tooth was embedded in epoxy resin with the dentin surface exposed. The specimens were ground with silicone carbide paper and the dentin cylinders were cemented onto the dentin surface of the other half of the same tooth.Materials: Resin cement and adhesive systems from three different manufacturers were used in various combinations (n = 8 per group). Cement and adhesive from the same manufacturer served as control. Shear bond strength (SBS) was measured and fracture modes were registered.Results and conclusions: The highest median SBS value was found in a bonding combination between cement and a non-corresponding adhesive (33.1 MPa) and one of the lowest values was found in one of the controls (15.3 MPa). Cohesive fractures were most frequent. The results indicated that combining adhesive and cement from different manufacturers did not compromise the dentin bonding. The novel test method is recommended for evaluating dentin bonding.

Objective: To assess whether composite polymer resin delivered in compules include pores and the possible effect on the amount of porosity in dental restorations.

Method and materials: Original compules containing unpolymerised composite polymer resin (CPR) were scanned in a micro-CT. Four products were examined, which comprised universal composites (Herculite XRV Ultra, Ceram.X Universal, Tetric Evo Ceram) and a flowable bulk-fill composite (SDR) (n = 10 per group). The pore size distribution and amount of porosity (vol.%) were estimated for the unpolymerized and polymerized material used to restore a standardised cavity in a typodont tooth. Manufacturers' instructions were followed regarding material handling, and polymerisation by use of a calibrated light-curing unit. The pore characteristics and their size distribution, and the amount of porosity in the dental restoration were contrasted with the values measured in the compule. Non-parametric tests were used to analyse differences between the four products.

Results: All the composite polymer resin compules contained unpolymerised material that included pores. The universal composite compules included pores predominantly in the sub-100 µm sizes. In contrast, the flowable bulk-fill compules included a few pores with a diameter >100 µm, which were assumed to be air-bubbles. The unpolymerised material within the compule included consistently more pores compared to the extruded portion from the compule tip, and in the final restoration (p < .001). The amount of porosity in the restorations differed amongst the tested materials, with the flowable bulk-fill composite showing the lowest amount of porosity (p < .01).

Purpose: By combining the increased toughness of a resin composite reinforced with discontinuous fibers and the surface wear resistance of a particulate filler composite (PFC), a bilayered composite technique was recently introduced. This study aimed to evaluate the effect of the thickness of the overlaying PFC placed over a fiber-reinforced composite (FRC) core, on the fracture-behavior of direct crown restorations. Methods: Six groups of posterior crown restorations (n = 8/group) were fabricated having a discontinuous FRC-core (everX Flow) and a layer of surface PFC (Essentia U) with various thicknesses (0.5, 1.0, 1.5, 2.0 mm), with the remaining thickness of the bilayered being 6 mm. Control groups were only made of PFC or FRC materials. Restorations were statically loaded until fracture. Failure-modes were visually examined. Data were analyzed using ANOVA (p = .05) and regression analysis. Results: The regression analysis showed that by decreasing the thickness of PFC layer, the load bearing capacity of restorations increased linearly (R²=0.7909). ANOVA revealed that crown restorations made only from everX Flow composite had significantly higher load-bearing capacities (3990 ± 331 N) (p < .05) among all the groups tested. With regard to the failure-mode analysis, crowns that had a FRC core material of everX Flow revealed delamination of the PFC surface composite from the core. Crowns which were made only of PFC i.e. with no fiber reinforcement, showed a crushing-like fracture pattern. Conclusions: Restorations combining a thick FRC-core and a thin surface layer of PFC (0.5-1 mm), displayed promising performance related to fracture-behavior and load-bearing capacity.

Objective: The aims of this in vitro study were to assess if dynamic loading increases the metal ion release of selected dental alloys and to evaluate the cytotoxicity of the released metal ions. Materials and methods: One Pd-Ag alloy (Aurolite 2B) and two Co-Cr alloys (Wirobond 280 and d.Sign 30) were investigated. Two different corrosion immersion tests were used: a standardized static test (ISO 22674: 2016) and an experimental dynamic test. Both tests involved immersion of the specimens in a lactic acidic solution (pH = 2.3). Inductively coupled plasma mass spectrometry was used to identify and quantify released elements. A human monocyte cell-line (THP-1) was exposed to serially diluted solutions containing the selected metal ions. Cell viability was measured using the methyl-thiazolyl-tetrazolium assay. Results: According to the threshold defined in ISO 22674, only low concentrations of released elements were observed for both corrosion tests. No increase in metal ion release from the dynamic test compared with the static test was observed. Of the released elements, only Zn(II) and Co(II) showed a cytotoxic effect on THP-1 cells at 250 µM and higher concentrations. No increased viability loss was observed when adding other released elements to the exposure mixture. Conclusions: The tested alloys showed low levels of metal ion release from both static and dynamic corrosion testing. Dynamic loading did not increase the metal ion release compared to the static corrosion test. Concentrations of 250 µM and above of Zn(II) and Co(II) showed a cytotoxic effect on THP-1 cells.

Objective: Ceramic inlay-retained fixed partial denture (IRFPD) is a conservative prosthetic option but the mechanical durability of new high strength zirconia reinforced glass ceramic FPDs is not investigated. The purpose of this study was to compare fracture load of 3-unit ceramic FPDs. Materials and methods: Extracted premolars and molars (N = 64) were used to create three test groups (IRFPDs) and one control group (full coverage FPD) (n = 8). The teeth were embedded in PMMA resin with a mesiodistal distance of 6 mm. Premolars had a distal and molars had a mesial inlay preparation (width: 3 mm; height: 4 mm) in the test groups. IRFPDs were made from a zirconia reinforced lithium silicate (VS) or a monolithic zirconia. Zirconia IRFPDs received 2 types of surface treatments: sandblasting (Zr-IRFPD) or internal coating with feldspathic porcelain (ZrC-IRFPD). Control group was made from monolithic zirconia with the same connector size and zirconia surfaces were sandblasted (Zr-FPD). All restorations were cemented using a resin luting cement. After 5000 thermo-cycles, fracture load values (N) were determined with a universal testing machine at a crosshead speed of 0.75 mm/min. Data were analyzed using 1-way ANOVA and Tukey`s post hoc test (p ˂ .05). Result: Fracture load (mean ± SD) of Zr-FPDs, Zr-IRFPDs and ZrC-IRFPDs were 672 ± 183, 672 ± 123 and 638 ± 59, respectively, being not statistically different (p > .05). VS-IRFPD exhibited statically lower values (391 ± 136). The predominant mode of failure was fracture at the connector area in all groups. Conclusion: The fracture load of 3-unit IRFPD was significantly affected by types of ceramics but the retainer design and surface treatment in Zr groups did not show a significant effect.