Biomaterial investigations in dentistry最新文献

Statement of the problem: Long-term success of ceramic veneers depends on the color stability of resin cement used for their cementation. Color change of cement may be seen through the ceramic and compromise esthetics.

Purpose: This study aimed to compare the color change of two resin cements and their visibility through the ceramic veneers after accelerated artificial aging.

Materials and methods: In this in vitro study, color change (ΔE) was measured in the following groups (n = 10) before and after accelerated artificial aging: group 1, IPS e.max press high translucent ceramic discs; groups 2, Variolink NLC resin cement discs; group 3, Choice 2 resin cement discs; groups 4, Variolink NLC discs bonded to e.max ceramic discs; group 5, Choice 2 disc bonded to e.max ceramic discs. Color change was measured using a spectrophotometer according to International Commission on Illumination Lab (CIELab). Data were analyzed using one-way ANOVA and Tukey's post-hoc test.

Results: Group 2 showed the highest (ΔE = 10.4 ± 0.9) and group 1 the lowest (ΔE = 0.9 ± 0.4) color change. The color change of Variolink was significantly greater than Choice 2 either alone (p < .001) or through the ceramic discs (p < .004). The color change of both cements was lower through the ceramic veneer and this reduction was statistically significant (p < .001).

Conclusion: Noticeable color change may be expected in areas of cement exposure for both cements studied. Also, in case of using Variolink cement, the color change may be seen through the ceramic veneers.

Purpose: This study aimed to compare the risk of orthodontic mini-implant (OMI) failure between maxilla and mandible. A critical analysis of finite-element studies was used to explain the contradiction of the greatest clinical success for OMIs placed in the maxilla, despite the higher quality bone of mandible. Materials and Methods: Four tridimensional FE models were built, simulating an OMI inserted in a low-dense maxilla, control maxilla, control mandible, and high-dense mandible. A horizontal force was applied to simulate an anterior retraction of 2 N (clinical scenario) and 10 N (overloading condition). The intra-bone OMI displacement and the major principal bone strains were used to evaluate the risk of failure due to insufficient primary stability or peri-implant bone resorption. Results: The OMI displacement was far below the 50-100 µm threshold, suggesting that the primary stability would be sufficient in all models. However, the maxilla was more prone to lose its stability due to overload conditions, especially in the low-dense condition, in which major principal bone strains surpassed the pathologic bone resorption threshold of 3000 µstrain. Conclusions: The differences in orthodontic mini-implant failures cannot be explained by maxilla and mandible bone quality in finite-element analysis that does not incorporate the residual stress due to OMI insertion.

The significance of dental materials in dentin-pulp complex tissue engineering is undeniable. The mechanical properties and bioactivity of mineral trioxide aggregate (MTA) make it a promising biomaterial for future stem cell-based endodontic therapies. There are numerous in vitro studies suggesting the low cytotoxicity of MTA towards various types of cells. Moreover, it has been shown that MTA can enhance mesenchymal stem cells' (MSCs) osteo/odontogenic ability. According to the preferred reporting items for systematic reviews and meta-analyses (PRISMA), a literature review was conducted in the Medline, PubMed, and Scopus databases. Among the identified records, the cytotoxicity and osteo/odontoblastic potential of MTA or its extract on stem cells were investigated. Previous studies have discovered the differentiation-inducing potential of MTA on MSCs, providing a background for dentin-pulp complex cell therapies using the MTA, however, animal trials are needed before moving into clinical trials. In conclusion, MTA can be a promising candidate dental biomaterial for futuristic stem cell-based endodontic therapies.

Objective: Charcoal-containing dentifrices are gaining popularity, but scientific information on their effect on oral health is scarce. This study investigated properties of dentifrices that may affect dentine abrasivity, as well as their ability to adsorb fluoride, their pH and the presence of harmful substances.

Materials and methods: The dentifrices NAO and COCO were subjected to the following analyses: abrasivity, expressed as mean abraded depth and relative dentin abrasivity (RDA), and surface roughness of extracted human molars (n = 30) after simulated brushing; fluoride adsorption measured as concentration change; pH measurements; detection of polycyclic aromatic hydrocarbons by gas chromatography-mass spectrometry. The products were compared to a reference dentifrice (Colgate^® MaxWhite), positive controls (ISO dentifrice slurry, activated charcoal for laboratory use) and a negative control (distilled water).

Results: The mean abraded depths of NAO and COCO were not different (p > .05), but higher than the reference dentifrice and the negative control (p < .05). The RDA values of NAO, COCO and the ISO dentifrice slurry were higher than the reference dentifrice value (p < .05) by up to 10 times. The dentine surface roughness was higher after brushing with NAO, COCO and ISO dentifrice slurry compared to distilled water (p < .05). No change in mean adsorbed fluoride concentration was observed after 24 h (p > .05). Both NAO and COCO were alkaline (pH > 7). Analysis of NAO revealed the presence of naphthalene (112.8 ± 2.0 ng/mL).

Conclusion: The charcoal-containing dentifrices were abrasive within acceptable limits set by ISO and did not adsorb fluoride. The presence of naphthalene in one product is a cause for concern.

Aim: Resin composite (RC) are commonly used under full crowns. However, independent information is lacking to guide practitioners regarding core RC material selection. This study aimed at comparing the flexural properties of a large selection of commercially-available core build-up RCs (CBU-RC), either light-, self- or dual-cure, to conventional light-cure RCs.

Methods: RCs were injected into a 25 × 2×2mm Teflon mold, and either light-cured during 20 s (materials with claimed light-cure characteristics) or covered by aluminum during 10 min (dual- and self-cure CBU-RCs). They were subjected after a one-week water storage at 37.5 °C to three-point bending, and Flexural modulus (E _flex) and Flexural Strength (σ _f) were calculated (n = 20). Thermogravimetric analysis (n = 3) was performed to determine inorganic filler content (%).

Results: For dual-cure CBU-RCs, both RC (p < .0001) and light-curing (p = .0007) had a significant influence on E _flex, while only RC was significant for σ _f (p < .0001). Between all conventional RCs and CBU-RCs, significant differences were observed (p < .0001), both regarding E _flex and σ _f, with values ranging from 3.9 to 15.5 GPa and from 76 to 130.3 MPa, respectively. Higher E _flex values were observed for light-cure RCs than for self- and dual-cure ones, while no clear trend was noticed regarding σ _f. Good linear correlation was found between inorganic filler content and E _flex (R ²=0.85, p < .0001), but not with σ _f (R ²=0.08, p = .1609).

Conclusion: This work demonstrated a positive influence of light-curing on dual-cure CBU-RC's E _flex. It also highlighted large differences in flexural properties (especially E _flex) among the investigated materials, questioning the use of some CBU-RCs as dentin replacement in case of large tissue loss.

Objectives: To investigate if differences in titanium implant surface topography influence Candida albicans biofilm formation.

Materials and methods: Titanium discs were prepared and characterized using a profilometer: Group A (R _a 0.15 µm, smooth), Group B (R _a 0.64 µm, minimally rough) and Group C (R _a 1.3 µm, moderately rough). Contact angle and surface free energy (SFE) were determined for each group. Non-preconditioned titanium discs were incubated with C. albicans for 24 h. In additional experiments, the titanium discs were initially coated with human saliva, bovine serum albumin or phosphate-buffered saline for 2 h before incubation with C. albicans for 24 h. The amount of fungal biofilm formation was quantified using a colorimetric assay.

Results: C. albicans biofilm formation was significantly lower (p < 0.05) on the minimally rough titanium surface compared to smooth and moderately rough surfaces. The titanium surface displaying the lowest SFE (Group B) was associated with significantly lower (p < 0.05) C. albicans biofilm formation than the other two groups. Salivary coating resulted in greater adherence of C. albicans with increased surface roughness.

Conclusions: The minimally rough titanium discs displayed lowest SFE compared to smooth and moderately rough surfaces and showed the least C. albicans biofilm formation. This study demonstrated that C. albicans biofilm formation increased in a SFE-dependent manner. These findings suggest that SFE might be a more explanatory factor for C. albicans biofilm formation on titanium surfaces than roughness. The presence of a pellicle coating may negate the impact of SFE on C. albicans biofilm formation on titanium surfaces.

Objective: This study investigated the effect of surface nano-patterning on adhesion of an oral early commensal colonizer, Streptococcus mitis and the opportunistic pathogen Staphylococcus aureus and human fibroblasts (HDFa) in a laminar flow cell.

Methods: Nanostructured surfaces were made by functionalizing glass substrates with 40 nm SiO₂ nanoparticles. Gradients in nanoparticle surface coverage were fabricated to study the effect of nanoparticle spacing within a single experiment. Bacterial adhesion was investigated after 5 min of contact time by subjecting surfaces to a flow in a laminar flow cell. In addition, to examine the particles effect on human cells, the establishment of focal adhesion and spreading of primary human dermal fibroblasts (HDFa) were investigated after 4 and 24 h.

Results: Adhesion of both S. aureus and S. mitis decreased on surfaces functionalized with nanoparticles and coincided with higher nanoparticle surface coverage on the surface. Both strains were tested on three separate surfaces. The regression analysis showed that S. mitis was influenced more by surface modification than S. aureus. The establishment of focal adhesions in HDFa cells was delayed on the nanostructured part of the surfaces after both 4 and 24 h of culturing.

Significance: In the current manuscript, we have used a flow cell to investigate the effect of nanotopographies on S. aureus and S. mitis adhesion. The present findings are of relevance for design of future implant and prostheses surfaces in order to reduce adhesion of bacteria.

Purpose: High-translucent dental zirconia has been introduced as a suitable material for anterior monolithic restorations. The material composition differs from traditional 3Y-TZP both with regard to yttria content and grain size. Little is known regarding how these alterations affect other properties than translucency and flexural strength. The aim of this study was to evaluate the crack propagation resistance and hardness of dental zirconias with different yttria content and different manufacturing methods.

Materials and methods: Measurement of hardness (HV2/5) and crack propagation from the indents (damage tolerance) was performed using a hardness tester(Vicker) on a flat polished surface of five crowns from six different commercial dental zirconias; one hard-machined 3Y-TZP, three soft-machined 3-5% yttria-stabilized zirconias and two soft-machined zirconias with ≥5% yttria content.

Results: Damage control varied greatly among dental zirconias with different compositions and fabrication methods. The hard-machined 3Y-TZP had better crack propagation resistance than soft-machined, 3-5% yttria-stabilized zirconias.

Conslusion: The ultra-translucent zirconias with ≥5% yttria content had the lowest crack propagation resistance. Hardness is not a suitable indicator for damage tolerance.

Aim: This study aimed to assess the efficacy of cavity liners with/without the atmospheric cold plasma jet (ACPJ) for dentin remineralization.

Material and methods: The occlusal third of 24 extracted third molars was cut. An occlusal cavity was prepared in the dentin of each tooth with 1 mm depth and 2 mm diameter and demineralized with 37% phosphoric acid for 1 min. The teeth were randomly divided into 8 groups (n = 3). The first control group only underwent demineralization with phosphoric acid. The second control group underwent demineralization and helium plasma jet. Groups 3 to 5 were filled with calcium hydroxide (CH), RetroMTA (MTA) and Biodentine. Groups 6 to 8 were subjected to ACPJ, and all the groups were sealed with polycarboxylate. After 2 weeks of immersion in water, the teeth were longitudinally sectioned and their mineral content was analyzed using energy-dispersive X-ray spectroscopy (EDX).

Results: The interaction effect of ACPJ and type of liner was not significant (p > 0.05). Application of ACPJ in combination with liner had a significant effect on calcium and phosphorous contents (p < .05). The calcium and phosphorous contents in the control groups were significantly lower than other groups (p < .05). The calcium and phosphorous contents in the CH group were higher than the control but significantly lower than the MTA and Biodentine groups. The values in the MTA and Biodentine groups were the same and higher than other groups.

Conlusion: All three cavity liners significantly increased the calcium and phosphorous contents of dentin. This increase was significantly greater by the MTA and Biodentine and enhanced by the ACPJ.