Pub Date : 2020-08-11DOI: 10.31487/j.dobcr.2020.04.01
S. Teramoto
Oral care and oral management are important for preventing aspiration pneumonia (ASP). The main pathology of ASP is microaspiration of oropharyngeal contents during night, swallowing rehabilitation must be necessary for the ASP treatment. However, swallowing rehabilitation cannot totally restore the normal swallowing function. Oral healthcare management should be initiated and continued in parallel with antibiotic treatment of ASP. Oral care helps to prevent aspiration pneumonia by reducing oral bacteria, while oral management helps by improving masticatory and rehabilitating functions. The efficacy of oral care for reducing the incidence of pneumonia has been clearly observed in untreated frail elderly patients, but not in well-cared elderly persons. It has been established that oral care is cyclically significant for the prevention of ASP in the elderly. However, oral problems are not the primary cause of ASP in the elderly. Although there is an amount of evidences of oral care for ASP prevention, there are some controversies of the clinical significance of oral care for ASP treatment.
{"title":"Clinical Significance of Oral Care and Oral Management on the Treatment and Prevention of Aspiration Pneumonia in the Elderly: Evidences and Pitfalls","authors":"S. Teramoto","doi":"10.31487/j.dobcr.2020.04.01","DOIUrl":"https://doi.org/10.31487/j.dobcr.2020.04.01","url":null,"abstract":"Oral care and oral management are important for preventing aspiration pneumonia (ASP). The main\u0000pathology of ASP is microaspiration of oropharyngeal contents during night, swallowing rehabilitation must\u0000be necessary for the ASP treatment. However, swallowing rehabilitation cannot totally restore the normal\u0000swallowing function. Oral healthcare management should be initiated and continued in parallel with\u0000antibiotic treatment of ASP. Oral care helps to prevent aspiration pneumonia by reducing oral bacteria,\u0000while oral management helps by improving masticatory and rehabilitating functions. The efficacy of oral\u0000care for reducing the incidence of pneumonia has been clearly observed in untreated frail elderly patients,\u0000but not in well-cared elderly persons. It has been established that oral care is cyclically significant for the\u0000prevention of ASP in the elderly. However, oral problems are not the primary cause of ASP in the elderly.\u0000Although there is an amount of evidences of oral care for ASP prevention, there are some controversies of\u0000the clinical significance of oral care for ASP treatment.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41712840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-14DOI: 10.1101/2020.07.13.20120428
F. Germano, D. Testi, L. Campagnolo, M. Scimeca, C. Arcuri
Cell-wall-deficient bacteria are those that lack cell walls and live in a pleomorphic state. The genus Mycoplasma and L-form bacteria are both members of this group. The aim of this study was to search cell-wall-deficient bacteria in periodontal biofilm and link their presence to periodontal disease. Eighty-nine individuals were recruited and divided into three groups: periodontally healthy individuals, individuals with chronic periodontitis, and those with aggressive periodontitis. The presence of cell-wall-deficient bacteria was detected in freshly collected biofilm by light microscopy, transmission electron microscopy (TEM) with and without electron microscopy in situ hybridization, atomic force microscopy and DNA stain (Hoechst). A new dichotomic index of classification for prevalence and morphologic variants was developed to classify cell-wall-deficient bacteria in periodontal biofilm. Cell-wall-deficient bacteria were found in periodontal biofilm and classified into Protoplastic, Everted, Filament and Intracellular forms, the last one mainly associated with aggressive periodontitis. We also assessed the prevalence of periodontopathic bacteria by means of polymerase chain-reaction (PCR) and found no clear, statistically significant, correlation among periodontal pathogens tested (except T. denticola) that allowed individuals with chronic periodontitis to be distinguished from those with aggressive periodontitis. Association between cell-wall-deficient bacteria and periodontal condition was: periodontally healthy, 3.3% (1/30); individuals with chronic periodontitis, 30.6% (11/36); and those with aggressive periodontitis, 100% (23/23). Cell-wall-deficient bacteria were detected in periodontal biofilm and linked to aggressive periodontitis.
{"title":"Cell-wall-deficient Bacteria in Oral Biofilm: Association with Periodontitis","authors":"F. Germano, D. Testi, L. Campagnolo, M. Scimeca, C. Arcuri","doi":"10.1101/2020.07.13.20120428","DOIUrl":"https://doi.org/10.1101/2020.07.13.20120428","url":null,"abstract":"Cell-wall-deficient bacteria are those that lack cell walls and live in a pleomorphic state. The genus Mycoplasma and L-form bacteria are both members of this group. The aim of this study was to search cell-wall-deficient bacteria in periodontal biofilm and link their presence to periodontal disease. Eighty-nine individuals were recruited and divided into three groups: periodontally healthy individuals, individuals with chronic periodontitis, and those with aggressive periodontitis. The presence of cell-wall-deficient bacteria was detected in freshly collected biofilm by light microscopy, transmission electron microscopy (TEM) with and without electron microscopy in situ hybridization, atomic force microscopy and DNA stain (Hoechst). A new dichotomic index of classification for prevalence and morphologic variants was developed to classify cell-wall-deficient bacteria in periodontal biofilm. Cell-wall-deficient bacteria were found in periodontal biofilm and classified into Protoplastic, Everted, Filament and Intracellular forms, the last one mainly associated with aggressive periodontitis. We also assessed the prevalence of periodontopathic bacteria by means of polymerase chain-reaction (PCR) and found no clear, statistically significant, correlation among periodontal pathogens tested (except T. denticola) that allowed individuals with chronic periodontitis to be distinguished from those with aggressive periodontitis. Association between cell-wall-deficient bacteria and periodontal condition was: periodontally healthy, 3.3% (1/30); individuals with chronic periodontitis, 30.6% (11/36); and those with aggressive periodontitis, 100% (23/23). Cell-wall-deficient bacteria were detected in periodontal biofilm and linked to aggressive periodontitis.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45377304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.31487/j.dobcr.2020.02.07
Ana Katarine Almeida Rios, Cristina Cangussu, Jakeline Martins Novaes Pedreira, Mércia Sacramento dos Santos, Thais Feitosa Leitão de Oliveira
Introduction: Periodic dental consultations and oral self-examination can increase the chances of an early diagnosis of oral cancer. However, the restricted population access to health services in Brazil added to the greater scope of the internet has increased the use of this resource as a source of information on signs, symptoms and treatments of several diseases, including oral cancer. Objective: To assess the reliability of the information on self-examination and oral cancer available on the web in Portuguese for Brazilian population. Methodology: In December 2018, independent searches were performed for the terms "mouth cancer", "oral cancer", "oral self- examination/ self-examination" and "buccal self- examination / self-examination", on Google Search (Portuguese terms). The first fifty pages were selected for each term, and those that included prevention, diagnosis of oral cancer or described verbally and visually the oral self-examination were included in the study. The quality of the information was assessed according to the criteria of the American Medical Association and Health on the Net. Of the 108 sites evaluated, 87% did not inform the author's training, 24.1% did not inform the complementary nature of the information and 64.8% provided guidance consult a doctor or dentist. Scientific references were not present in 39.8% of the sites and the “accurate and complete” content was observed in only 35.2% of the evaluated pages. Conclusion: The low quality of the information available on the evaluated pages, suggests low reliability of the content about oral cancer and oral self-examination available on the web, indicating that access to this content may bring risk to the health of the Brazilian population.
{"title":"Reliability of Online Information on Self-Examination and Oral Cancer","authors":"Ana Katarine Almeida Rios, Cristina Cangussu, Jakeline Martins Novaes Pedreira, Mércia Sacramento dos Santos, Thais Feitosa Leitão de Oliveira","doi":"10.31487/j.dobcr.2020.02.07","DOIUrl":"https://doi.org/10.31487/j.dobcr.2020.02.07","url":null,"abstract":"Introduction: Periodic dental consultations and oral self-examination can increase the chances of an early\u0000diagnosis of oral cancer. However, the restricted population access to health services in Brazil added to the\u0000greater scope of the internet has increased the use of this resource as a source of information on signs,\u0000symptoms and treatments of several diseases, including oral cancer.\u0000Objective: To assess the reliability of the information on self-examination and oral cancer available on the\u0000web in Portuguese for Brazilian population.\u0000Methodology: In December 2018, independent searches were performed for the terms \"mouth cancer\",\u0000\"oral cancer\", \"oral self- examination/ self-examination\" and \"buccal self- examination / self-examination\",\u0000on Google Search (Portuguese terms). The first fifty pages were selected for each term, and those that\u0000included prevention, diagnosis of oral cancer or described verbally and visually the oral self-examination\u0000were included in the study. The quality of the information was assessed according to the criteria of the\u0000American Medical Association and Health on the Net. Of the 108 sites evaluated, 87% did not inform the\u0000author's training, 24.1% did not inform the complementary nature of the information and 64.8% provided\u0000guidance consult a doctor or dentist. Scientific references were not present in 39.8% of the sites and the\u0000“accurate and complete” content was observed in only 35.2% of the evaluated pages.\u0000Conclusion: The low quality of the information available on the evaluated pages, suggests low reliability\u0000of the content about oral cancer and oral self-examination available on the web, indicating that access to\u0000this content may bring risk to the health of the Brazilian population.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47717100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.31487/j.dobcr.2020.03.02
E. Delgado, Isabel Dias
Periodontal disease is a high prevalent and multi-factorial oral disease in dogs and ultrasonic scaling is used to remove dental plaque and calculus, releasing contaminated aerosols, which may represent a hazard to animal and human health. This study aimed to identify the microorganisms present in aerosols produced during dental scaling of canine patients. A random sample of 15 dogs with periodontal disease was included, and aerosol samples were collected, incubated, and isolated bacteria were identified. Dogs without previous antibiotic treatment (n=4) and dogs that received systemic antibiotics up to two weeks before to dental scaling and polishing, either amoxicillin and clavulanic acid (n=3), or a combination of metronidazole and spiramycin (n=8), were included in the study. The highest percentage of pathogenic bacteria present corresponded to Pseudomonas spp. (20%), followed by Staphylococcus spp. (13%) and Escherichia coli (10%). The most prevalent bacteria identified in the non-treated group was Pseudomonas spp. Within the group subject to prior antibiotherapy, the predominant bacterial species was also Pseudomonas spp. Followed by Staphylococcus spp. In spite of previous antibiotherapy, strong bacterial contamination was still present, suggesting that this is not a warranty of less contamination of the released aerosols. The bacteria identified in this study represent a serious hazard to public and animal health, so strict hygiene and prevention measures during ultrasonic scaling in dogs are mandatory.
{"title":"Bacterial Aerosols Released During Dental Ultrasonic Scaling in Dogs","authors":"E. Delgado, Isabel Dias","doi":"10.31487/j.dobcr.2020.03.02","DOIUrl":"https://doi.org/10.31487/j.dobcr.2020.03.02","url":null,"abstract":"Periodontal disease is a high prevalent and multi-factorial oral disease in dogs and ultrasonic scaling is used\u0000to remove dental plaque and calculus, releasing contaminated aerosols, which may represent a hazard to\u0000animal and human health. This study aimed to identify the microorganisms present in aerosols produced\u0000during dental scaling of canine patients. A random sample of 15 dogs with periodontal disease was included,\u0000and aerosol samples were collected, incubated, and isolated bacteria were identified. Dogs without previous\u0000antibiotic treatment (n=4) and dogs that received systemic antibiotics up to two weeks before to dental\u0000scaling and polishing, either amoxicillin and clavulanic acid (n=3), or a combination of metronidazole and\u0000spiramycin (n=8), were included in the study. The highest percentage of pathogenic bacteria present\u0000corresponded to Pseudomonas spp. (20%), followed by Staphylococcus spp. (13%) and Escherichia coli\u0000(10%). The most prevalent bacteria identified in the non-treated group was Pseudomonas spp. Within the\u0000group subject to prior antibiotherapy, the predominant bacterial species was also Pseudomonas spp.\u0000Followed by Staphylococcus spp. In spite of previous antibiotherapy, strong bacterial contamination was\u0000still present, suggesting that this is not a warranty of less contamination of the released aerosols. The bacteria\u0000identified in this study represent a serious hazard to public and animal health, so strict hygiene and\u0000prevention measures during ultrasonic scaling in dogs are mandatory.\u0000","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41735731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-19DOI: 10.31487/j.dobcr.2020.02.09
E. Marques, Raquel Silva, R. Hsieh
Lupus Erythematosus (LE) is a chronic autoimmune inflammatory disease, characterized by a wide spectrum of manifestations of variable evolution. Among the various organs involved, the skin is one of the most frequent site, sometimes being the first manifestation of the disease. The frequency of oral mucosal manifestations is variable, between 9 to 45% of SLE and 3 to 20% of Chronic Cutaneous Lupus Erythematosus. Oral mucosal manifestation of LE can present themselves in different ways and their description is varied in different studies. Oral Lupus Erythematosus is histopathologically characterized as an interface mucositis with hyperkeratosis, alternating epithelial hyperplasia with atrophy, changes in epithelial maturation, vacuolization of the basal layer, thickening of the basement membrane by Schiff's periodic acid staining and superficial and deep lymph-histiocytic infiltrate in the lamina propria, which can show focal, interstitial, perivascular location or in a tight epithelial band. The etiopathogenesis of LE is the result of a complex interaction between various pathogenic factors and remains unclear. Several mechanisms are known to participate in the process, such as: genetic, hormonal, environmental, autoantibodies and cellular components of the skin and the immune system. The treatment of LE, including oral mucosal manifestation, is a complex and multidisciplinary process, which is based on preventive measures, inflammatory process modulation and control, organ injury prevention, relief of the symptoms. The oral mucosal manifestation of LE is a rare condition, it could be observed and diagnosed by many health professionals, mainly by dentist, stomatologist, dermatologist and otolaryngologist, knowledge and depth study of OLE is needed by these professionals, thus, the sooner the disease is diagnosed, the better prognosis and quality of life can be given to the patient.
{"title":"Oral Mucosal Manifestation of Lupus Erythematosus: A Short Review","authors":"E. Marques, Raquel Silva, R. Hsieh","doi":"10.31487/j.dobcr.2020.02.09","DOIUrl":"https://doi.org/10.31487/j.dobcr.2020.02.09","url":null,"abstract":"Lupus Erythematosus (LE) is a chronic autoimmune inflammatory disease, characterized by a wide\u0000spectrum of manifestations of variable evolution. Among the various organs involved, the skin is one of the\u0000most frequent site, sometimes being the first manifestation of the disease. The frequency of oral mucosal\u0000manifestations is variable, between 9 to 45% of SLE and 3 to 20% of Chronic Cutaneous Lupus\u0000Erythematosus. Oral mucosal manifestation of LE can present themselves in different ways and their\u0000description is varied in different studies. Oral Lupus Erythematosus is histopathologically characterized as\u0000an interface mucositis with hyperkeratosis, alternating epithelial hyperplasia with atrophy, changes in\u0000epithelial maturation, vacuolization of the basal layer, thickening of the basement membrane by Schiff's\u0000periodic acid staining and superficial and deep lymph-histiocytic infiltrate in the lamina propria, which can\u0000show focal, interstitial, perivascular location or in a tight epithelial band. The etiopathogenesis of LE is the\u0000result of a complex interaction between various pathogenic factors and remains unclear. Several\u0000mechanisms are known to participate in the process, such as: genetic, hormonal, environmental,\u0000autoantibodies and cellular components of the skin and the immune system. The treatment of LE, including\u0000oral mucosal manifestation, is a complex and multidisciplinary process, which is based on preventive\u0000measures, inflammatory process modulation and control, organ injury prevention, relief of the symptoms.\u0000The oral mucosal manifestation of LE is a rare condition, it could be observed and diagnosed by many health\u0000professionals, mainly by dentist, stomatologist, dermatologist and otolaryngologist, knowledge and depth\u0000study of OLE is needed by these professionals, thus, the sooner the disease is diagnosed, the better prognosis\u0000and quality of life can be given to the patient.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42500819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-18DOI: 10.31487/j.dobcr.2020.03.01
J. Hatton, K. Kumar, D. Gillam
Aim: The purpose of the present questionnaire-based study was to evaluate the knowledge and understanding of UK based dental undergraduates and qualified dentists in treating Dentine Hypersensitivity (DH). Methods: 120 questionnaires were handed out to 4th and 5th year dental students and Staff at the Dental Hospital in London UK as part of a collaborative study with the Faculdade de Odontologia de Bauru, University of Sao Paulo, Brazil. The questionnaire consisted of 22 questions, which included both open and closed questions. Results: 91 questionnaires (75.8% response rate) were returned; of the 91 respondents (38M; 52F, 1 missing value) 53 were dentists (61%) and 34 were dental students (39%) (4 missing values). 37.5% of students indicated that 10% of patients suffered from DH whereas 18.9% of dentists indicated that 25% of patients suffered from DH. Both dentists (22.6%) and 27.5% of students indicated that DH lasted >12 weeks. 18.9% of dentists considered that DH was a serious problem for patients although 32.5% of students were not sure. Dentists (66%) and students (62.5%) indicated that DH had a major impact on the quality of life (QOL) with 51.1% (dentists) and 56.3% (students) indicating that it was moderate in nature. Conclusion: The results of the present pilot study would suggest that in terms of knowledge and understanding of DH (e.g., hydrodynamic theory) both dentists (90.5%) and students (76.9%) were comparable although in the assessment and subsequent management of DH the results indicated that dentists were more confident than the students.
{"title":"Knowledge of UK Dental Undergraduates and Dentists in Treating Dentine Hypersensitivity","authors":"J. Hatton, K. Kumar, D. Gillam","doi":"10.31487/j.dobcr.2020.03.01","DOIUrl":"https://doi.org/10.31487/j.dobcr.2020.03.01","url":null,"abstract":"Aim: The purpose of the present questionnaire-based study was to evaluate the knowledge and\u0000understanding of UK based dental undergraduates and qualified dentists in treating Dentine Hypersensitivity\u0000(DH).\u0000Methods: 120 questionnaires were handed out to 4th and 5th year dental students and Staff at the Dental\u0000Hospital in London UK as part of a collaborative study with the Faculdade de Odontologia de Bauru,\u0000University of Sao Paulo, Brazil. The questionnaire consisted of 22 questions, which included both open and\u0000closed questions.\u0000Results: 91 questionnaires (75.8% response rate) were returned; of the 91 respondents (38M; 52F, 1 missing\u0000value) 53 were dentists (61%) and 34 were dental students (39%) (4 missing values). 37.5% of students\u0000indicated that 10% of patients suffered from DH whereas 18.9% of dentists indicated that 25% of patients\u0000suffered from DH. Both dentists (22.6%) and 27.5% of students indicated that DH lasted >12 weeks. 18.9%\u0000of dentists considered that DH was a serious problem for patients although 32.5% of students were not sure.\u0000Dentists (66%) and students (62.5%) indicated that DH had a major impact on the quality of life (QOL)\u0000with 51.1% (dentists) and 56.3% (students) indicating that it was moderate in nature.\u0000Conclusion: The results of the present pilot study would suggest that in terms of knowledge and\u0000understanding of DH (e.g., hydrodynamic theory) both dentists (90.5%) and students (76.9%) were\u0000comparable although in the assessment and subsequent management of DH the results indicated that dentists\u0000were more confident than the students.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42276042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-15DOI: 10.31487/j.dobcr.2020.03.04
F. F. E. Torres, J. C. Pinto, J. Guerreiro-Tanomaru, M. C. Pereira, M. T. Filho, Pedro H. F. de Souza
Aim: To evaluate flow, filling ability, and apical extrusion of three calcium silicate-based sealers, Neo MTA Plus (Avalon, USA), Bio-C Sealer (Angelus, Brazil) and Sealer Plus BC (MK Life, Brazil), in comparation with the gold standard based on epoxy resin, AH Plus (Dentsply, Germany). Methodology: The flow was evaluated based on ISO 6876/2012 standard (mm) and in area (mm2 ). For assessment of filling ability and apical extrusion of the sealers, curved artificial canals were prepared up to size 25.06, and filled with the sealers by the single cone technique. The samples were scanned in microcomputed tomography (SkyScan 1272. Bruker, Belgium) after preparation and after filling of the root canals. The percentage of voids throughout the entire extension of the root canals and in the apical third, besides the volume of apical extrusion of each sealer were calculated. Data were statistically analyzed using ANOVA/Tukey tests (α = 0.05). Results: Bio-C Sealer showed the highest flow and NeoMTA Plus had the lowest value (p < 0.05). Bio-C Sealer had lower percentage of total voids than NeoMTA Plus (p < 0.05). AH Plus had greater percentage of voids in the apical third than Bio-C Sealer and Sealer Plus BC (p < 0.05). Bio-C Sealer showed higher volume of extrusion (p < 0.05) than AH Plus and NeoMTA Plus. Conclusion: Bio-C Sealer and Sealer Plus BC had greater flow and proper filling ability in the apical third. However, these sealers presented high volume of apical extrusion. NeoMTA Plus provided less sealer extrusion, low flow, and more presence of voids.
目的:比较三种硅酸钙基密封剂Neo MTAPlus (Avalon，美国)、Bio-C Sealer (Angelus，巴西)和Sealer Plus BC (MK Life，巴西)与环氧树脂AH Plus (Dentsply，德国)的金标准密封剂的流动性、填充能力和根顶挤出性。方法:根据ISO 6876/2012标准(mm)和面积(mm2)对流量进行评估。为了评价封闭体的充填能力和根尖挤压能力，我们制备了直径为25.06的弯曲人工管，并采用单锥技术填充封闭体。样品在微计算机断层扫描(SkyScan 1272)中进行扫描。Bruker，比利时)准备和填充根管后。除计算每个封闭器的根尖挤压体积外，还计算整个根管延伸部分和根尖三分之一的空隙百分比。资料采用anova /Tukey检验进行统计学分析(α = 0.05)。结果:Bio-C Sealer的流量最高，NeoMTA Plus的流量最低(p < 0.05)。Bio-CSealer的总空隙率低于NeoMTA Plus (p < 0.05)。AH Plus在根尖三分之一的空隙率高于Bio-C Sealer和Sealer Plus BC (p < 0.05)。Bio-C Sealer的挤压体积高于AH Plus和NeoMTA Plus (p < 0.05)。结论:Bio-C Sealer和Sealer Plus BC在根尖三分之一处具有更大的流量和适当的填充能力。然而，这些封口器表现出高容量的根尖挤压。NeoMTA Plus提供了更少的密封性挤压，低流量和更多的空隙。
{"title":"Flow, Filling Ability and Apical Extrusion of New Calcium Silicate-Based Sealers: A Micro-Computed Tomographic Study","authors":"F. F. E. Torres, J. C. Pinto, J. Guerreiro-Tanomaru, M. C. Pereira, M. T. Filho, Pedro H. F. de Souza","doi":"10.31487/j.dobcr.2020.03.04","DOIUrl":"https://doi.org/10.31487/j.dobcr.2020.03.04","url":null,"abstract":"Aim: To evaluate flow, filling ability, and apical extrusion of three calcium silicate-based sealers, Neo MTA Plus (Avalon, USA), Bio-C Sealer (Angelus, Brazil) and Sealer Plus BC (MK Life, Brazil), in comparation with the gold standard based on epoxy resin, AH Plus (Dentsply, Germany). Methodology: The flow was evaluated based on ISO 6876/2012 standard (mm) and in area (mm2 ). For assessment of filling ability and apical extrusion of the sealers, curved artificial canals were prepared up to size 25.06, and filled with the sealers by the single cone technique. The samples were scanned in microcomputed tomography (SkyScan 1272. Bruker, Belgium) after preparation and after filling of the root canals. The percentage of voids throughout the entire extension of the root canals and in the apical third, besides the volume of apical extrusion of each sealer were calculated. Data were statistically analyzed using ANOVA/Tukey tests (α = 0.05). Results: Bio-C Sealer showed the highest flow and NeoMTA Plus had the lowest value (p < 0.05). Bio-C Sealer had lower percentage of total voids than NeoMTA Plus (p < 0.05). AH Plus had greater percentage of voids in the apical third than Bio-C Sealer and Sealer Plus BC (p < 0.05). Bio-C Sealer showed higher volume of extrusion (p < 0.05) than AH Plus and NeoMTA Plus. Conclusion: Bio-C Sealer and Sealer Plus BC had greater flow and proper filling ability in the apical third. However, these sealers presented high volume of apical extrusion. NeoMTA Plus provided less sealer extrusion, low flow, and more presence of voids.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45067088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-29DOI: 10.31487/j.dobcr.2020.02.06
Ann Smith, D. Williams, D. Bradshaw, P. Milward, Sarah Al Kutubi, W. Rowe
Objectives: In the UK, 19% of adults wear dentures. Failure to keep a denture clean can lead to staining from foods, along with subsequent colonisation of the denture and associated mucosa by microorganisms, particularly Candida albicans. This colonisation can potentially lead to chronic erythematous candidosis and other oral infections. This study investigated the association between staining of denture acrylics by different food types and subsequent C. albicans colonisation. Materials and Methods: Chemically polymerised acrylic specimens were produced and stained for 14 days with six different combinations of food stains. The level of acrylic staining was determined spectrophotometrically. Specimens were then incubated in Sabouraud-dextrose broth (SAB) or SAB inoculated with Candida albicans. Confocal laser scanning microscopy coupled with propidium iodide staining of C. albicans was used to determine the extent of C. albicans colonisation to these acrylics. Results analysed descriptively and by one-way analysis of variance (ANOVA), one sample student t-test, and Dunnett’s test. Results: Acrylics in Group 4 (stained with spices, tomato puree, acai berry juice and sunflower oil) exhibited highest staining but had low C. albicans colonisation. Highest C. albicans colonisation occurred with Group 5 (sunflower oil) stained acrylics. The unstained control acrylic group had lowest colonisation. Conclusion: This study demonstrated that staining acrylics with certain foods promoted C. albicans colonisation, but this was not associated with level of visual staining. Further research is required to determine the precise mechanism(s) by which residual food stains promote candidal colonisation on denture acrylics. This knowledge may then be used by dental professionals to advise patients on improving denture hygiene to improve not only denture aesthetics but also minimise Candida biofilms.
{"title":"The Effect of Residual Food Stain on Candida Albicans Colonisation of Denture Acrylics","authors":"Ann Smith, D. Williams, D. Bradshaw, P. Milward, Sarah Al Kutubi, W. Rowe","doi":"10.31487/j.dobcr.2020.02.06","DOIUrl":"https://doi.org/10.31487/j.dobcr.2020.02.06","url":null,"abstract":"Objectives: In the UK, 19% of adults wear dentures. Failure to keep a denture clean can lead to staining\u0000from foods, along with subsequent colonisation of the denture and associated mucosa by microorganisms,\u0000particularly Candida albicans. This colonisation can potentially lead to chronic erythematous candidosis\u0000and other oral infections. This study investigated the association between staining of denture acrylics by\u0000different food types and subsequent C. albicans colonisation.\u0000Materials and Methods: Chemically polymerised acrylic specimens were produced and stained for 14 days\u0000with six different combinations of food stains. The level of acrylic staining was determined\u0000spectrophotometrically. Specimens were then incubated in Sabouraud-dextrose broth (SAB) or SAB\u0000inoculated with Candida albicans. Confocal laser scanning microscopy coupled with propidium iodide\u0000staining of C. albicans was used to determine the extent of C. albicans colonisation to these acrylics. Results\u0000analysed descriptively and by one-way analysis of variance (ANOVA), one sample student t-test, and\u0000Dunnett’s test.\u0000Results: Acrylics in Group 4 (stained with spices, tomato puree, acai berry juice and sunflower oil)\u0000exhibited highest staining but had low C. albicans colonisation. Highest C. albicans colonisation occurred\u0000with Group 5 (sunflower oil) stained acrylics. The unstained control acrylic group had lowest colonisation.\u0000Conclusion: This study demonstrated that staining acrylics with certain foods promoted C. albicans\u0000colonisation, but this was not associated with level of visual staining. Further research is required to\u0000determine the precise mechanism(s) by which residual food stains promote candidal colonisation on denture\u0000acrylics. This knowledge may then be used by dental professionals to advise patients on improving denture\u0000hygiene to improve not only denture aesthetics but also minimise Candida biofilms.","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69831804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-25DOI: 10.31487/j.dobcr.2020.02.10
J. Castillo, M. Sánchez, M. Herrera, M. Ruiz, P. Anguita, V. Bonilla
There are no recent studies that have evaluated the epidemiological relationship between dental manipulations the etiology of IE by these microorganisms, basically Streptococcus viridans. Our objective is to analyse the relationship between these variables and the possible therapeutic implications. For this, we have analysed a prospective single-center series of IE in patients not addicted to parenteral drugs collected and followed between 1987 and 2018. 403 cases of native and late prosthetic IE were diagnosed and followed up in our center. Of them, 91 were produced by oral streptococci (22.6%). The percentage of this microorganism has remained constant throughout the 30 years (24.8% of the cases from 1987-1997, 25% of those from 1998-2007 and 19.1% from 2008-2018. Although there was a history of dental manipulation in a greater proportion in cases of IE due to Streptococcus viridans (24% vs. 6.5%, p <0.001), in most cases due to Streptococcus viridans (76% of them) there was no previous dental manipulation. In these cases, the infection may be due to the usual daily manipulations of the mouth (brushing, dental floss) or periodontal disease. Given this low sensitivity, it cannot be ruled out that IE is caused by this microorganism in the absence of a history of visiting the dentist, with the implications for empirical antibiotic treatment that this entails
{"title":"Infective Endocarditis Caused by Oral Microorganisms and Entrance Door and Previous Dental Procedures: Does a Relationship Exists?","authors":"J. Castillo, M. Sánchez, M. Herrera, M. Ruiz, P. Anguita, V. Bonilla","doi":"10.31487/j.dobcr.2020.02.10","DOIUrl":"https://doi.org/10.31487/j.dobcr.2020.02.10","url":null,"abstract":"There are no recent studies that have evaluated the epidemiological relationship between dental manipulations the etiology of IE by these microorganisms, basically Streptococcus viridans. Our objective is to analyse the relationship between these variables and the possible therapeutic implications. For this, we have analysed a prospective single-center series of IE in patients not addicted to parenteral drugs collected and followed between 1987 and 2018. 403 cases of native and late prosthetic IE were diagnosed and followed up in our center. Of them, 91 were produced by oral streptococci (22.6%). The percentage of this microorganism has remained constant throughout the 30 years (24.8% of the cases from 1987-1997, 25% of those from 1998-2007 and 19.1% from 2008-2018. Although there was a history of dental manipulation in a greater proportion in cases of IE due to Streptococcus viridans (24% vs. 6.5%, p <0.001), in most cases due to Streptococcus viridans (76% of them) there was no previous dental manipulation. In these cases, the infection may be due to the usual daily manipulations of the mouth (brushing, dental floss) or periodontal disease. Given this low sensitivity, it cannot be ruled out that IE is caused by this microorganism in the absence of a history of visiting the dentist, with the implications for empirical antibiotic treatment that this entails","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47380459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-22DOI: 10.31487/j.dobcr.2020.02.01
C. Bacci, Cerrato Alessia, M. Boccuto, R. Ragona, Zanette Gastone
Purpose: To describe the management of orosinusal pathology by combined transnasal and oral endoscopy. Methods: The 54-year-old patient underwent a dental and otolaryngological evaluation for left odontogenic maxillary sinusitis in relation to plausible dental follicular cyst of ectopic element 2.8. The symptoms reported at the time of access to the hospital were nasal obstruction and nocturnal rhonchopathy. In the ENT evaluation by videorhinoscopy with rigid optics, complex deviation of the nasal septum was revealed, without any evident formation or pathological secretion. On inspection of the oral cavity, the mucous membranes appeared unscathed. The previously extracted aleveolus of 1.6 was evident. The radiological examination, facial CT, revealed the left maxillary sinus almost completely occupied by a cystic appearance, with thin calcified walls and homogeneous content that had a dental element, probably the 2.8, which fenestrates the vestibular cortex of the lateral wall of the maxillary sinus. This lesion erodes the medial wall of the sinus, obliterating the ostiomeatal complex and imprinting the ipsilateral ethmoidal cells. Biohumoral tests showed normal coagulation parameters, indices of renal function, liver and ionemia. The patient under general anesthesia and oral intubation with a combined intervention of the left anterior FESS, intrasulcular flap from dental elements 2.7 to 2.3 with mesial releasing incision, moderate osteotomy, ectopic 2.8 extraction and enucleation of the cystic lesion with simultaneous closure of the orosinusal communication with advancement of the Bichat fat pad and closure by first intention. In the same session, the ENT moment is carried out transnasally for total left uncinectomy, medium antrostomy with the union of the natural ostium and the accessory ostium. Bilateral lower turbinoplasty with bipolar forceps. The patient was then controlled after 15 days and then six months, showing good healing and no signs of recurrence at the rhinoscopic check on the physical examination of the oral cavity. Results: Based on the clinical and radiological aspect, the diagnosis of a follicular dentigerous cyst (WHO 2017) covered by a multi-layered non-keratinized paving epithelium, with moderate chronic inflammation, including gigantocellular and cholesteric crystals, is reached from the microbiological and histological examination. Necrotic amorphous material coexists including rare hyphae and fungal spores, with mycotic and actinomycotic superinfection. Conclusions: The combined oral and nasal intervention, allowed by the collaboration between the oral surgeon and ENT, has made it possible to shorten the healing time and resolve the pathology without recurrence.
{"title":"Maxillary Sinusitis of Odontogenic Origin in Relation to Ectopic 2.8 Associated with Follicular Dentigerous Cyst – Combined Transnasal and Oral Endoscopic Approach: A Case Report","authors":"C. Bacci, Cerrato Alessia, M. Boccuto, R. Ragona, Zanette Gastone","doi":"10.31487/j.dobcr.2020.02.01","DOIUrl":"https://doi.org/10.31487/j.dobcr.2020.02.01","url":null,"abstract":"Purpose: To describe the management of orosinusal pathology by combined transnasal and oral endoscopy.\u0000Methods: The 54-year-old patient underwent a dental and otolaryngological evaluation for left odontogenic\u0000maxillary sinusitis in relation to plausible dental follicular cyst of ectopic element 2.8. The symptoms\u0000reported at the time of access to the hospital were nasal obstruction and nocturnal rhonchopathy. In the ENT\u0000evaluation by videorhinoscopy with rigid optics, complex deviation of the nasal septum was revealed,\u0000without any evident formation or pathological secretion. On inspection of the oral cavity, the mucous\u0000membranes appeared unscathed. The previously extracted aleveolus of 1.6 was evident. The radiological\u0000examination, facial CT, revealed the left maxillary sinus almost completely occupied by a cystic appearance,\u0000with thin calcified walls and homogeneous content that had a dental element, probably the 2.8, which\u0000fenestrates the vestibular cortex of the lateral wall of the maxillary sinus. This lesion erodes the medial wall\u0000of the sinus, obliterating the ostiomeatal complex and imprinting the ipsilateral ethmoidal cells. Biohumoral\u0000tests showed normal coagulation parameters, indices of renal function, liver and ionemia. The patient under\u0000general anesthesia and oral intubation with a combined intervention of the left anterior FESS, intrasulcular\u0000flap from dental elements 2.7 to 2.3 with mesial releasing incision, moderate osteotomy, ectopic 2.8\u0000extraction and enucleation of the cystic lesion with simultaneous closure of the orosinusal communication\u0000with advancement of the Bichat fat pad and closure by first intention. In the same session, the ENT moment\u0000is carried out transnasally for total left uncinectomy, medium antrostomy with the union of the natural\u0000ostium and the accessory ostium. Bilateral lower turbinoplasty with bipolar forceps. The patient was then\u0000controlled after 15 days and then six months, showing good healing and no signs of recurrence at the\u0000rhinoscopic check on the physical examination of the oral cavity.\u0000Results: Based on the clinical and radiological aspect, the diagnosis of a follicular dentigerous cyst (WHO\u00002017) covered by a multi-layered non-keratinized paving epithelium, with moderate chronic inflammation,\u0000including gigantocellular and cholesteric crystals, is reached from the microbiological and histological\u0000examination. Necrotic amorphous material coexists including rare hyphae and fungal spores, with mycotic\u0000and actinomycotic superinfection.\u0000Conclusions: The combined oral and nasal intervention, allowed by the collaboration between the oral\u0000surgeon and ENT, has made it possible to shorten the healing time and resolve the pathology without\u0000recurrence.\u0000","PeriodicalId":72781,"journal":{"name":"Dental Oral Biology and Craniofacial Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46330386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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