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Screen-Printed Silver/Silver Chloride Electrodes Inhibit Alcohol Oxidase Activity. 丝网印刷银/氯化银电极抑制酒精氧化酶活性
Pub Date : 2023-09-01 DOI: 10.1149/2754-2726/ace5a9
Bob M Lansdorp, Peter Lamberg, Rashad Hamid

Silver/silver chloride (Ag/AgCl) is ubiquitous in the field of electrochemical biosensing due to its suitability as a reference electrode material. However, we recently discovered that screen-printed Ag/AgCl ink has a detrimental effect on Alcohol Oxidase enzyme stability. We performed an optical absorbance assay to isolate the interaction of enzyme and electrode to discover a surprisingly strong inhibition effect. The halftime of enzymatic activity was reduced from nearly 1 week in buffer to 10 h in the presence of the Ag/AgCl electrode. We expect this discovery to have broad implications on enzymatic biosensors that use Ag/AgCl as reference electrode material.

银/氯化银(Ag/AgCl)作为一种合适的参比电极材料,在电化学生物传感领域有着广泛的应用。然而,我们最近发现丝网印刷的Ag/AgCl油墨对酒精氧化酶的稳定性有不利影响。我们进行了光学吸光度测定,以分离酶和电极的相互作用,发现了令人惊讶的强抑制作用。在Ag/AgCl电极存在下,酶活性的半衰期从缓冲液中的近1周减少到10小时。我们希望这一发现对使用Ag/AgCl作为参比电极材料的酶促生物传感器具有广泛的意义。
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引用次数: 0
Reliable Gamma-Interconnection Network for Data Analysis in Sensor Networks: Design and Performance Evaluation 传感器网络中用于数据分析的可靠Gamma互连网络:设计和性能评估
Pub Date : 2023-08-23 DOI: 10.1149/2754-2726/acf328
Shilpa Gupta
In today’s era of high speed 5G internet all electronic sensor networks are connected through IoT. Bank transactions are digitized, people can access any data through their mobile phones, organizations and companies handle their projects through online meetings etc. Military and medical surveillance, navy navigation, weapon controlling, weather forecasting etc. involve big data analysis collected from sensors, that too at a very high speed with reliable results. This requires large number of parallel processors connected with huge Bank of memory modules to store big data. Reliable interconnection network is needed to connect these large number of parallel processors and memory modules efficiently hence Multistage Interconnection Networks (MINs) come into play, as they provide highly reliable communication for big data transfer between processors and memory modules whenever required. In this manuscript a new network named Reliable Gamma-interconnection Network (RGN) is introduced which possesses multiple paths between processors and memory modules with two totally disjoint path availability. It provides high reliability and minimum path distance between source node to destination node than other gamma networks known, with the minimum hardware complexity. Reliability estimation and evaluation of RGN has been presented in this paper and comparison of results achieved with other gamma networks has been done for validation purpose.
在当今高速5G互联网时代,所有电子传感器网络都通过物联网连接。银行交易是数字化的,人们可以通过手机访问任何数据,组织和公司通过在线会议处理项目等。军事和医疗监控、海军导航、武器控制、天气预报等都涉及从传感器收集的大数据分析,而且速度非常快,结果可靠。这需要大量的并行处理器与庞大的内存模块相连来存储大数据。需要可靠的互连网络来有效地连接这些大量的并行处理器和存储器模块,因此多级互连网络(MIN)开始发挥作用,因为它们在需要时为处理器和存储器模件之间的大数据传输提供高度可靠的通信。本文介绍了一种新的网络,称为可靠伽玛互连网络(RGN),它在处理器和存储器模块之间具有多条路径,具有两条完全不相交的路径可用性。与已知的其他伽马网络相比,它提供了高可靠性和源节点到目的节点之间的最小路径距离,并具有最小的硬件复杂性。本文介绍了RGN的可靠性估计和评估,并将所获得的结果与其他伽马网络进行了比较,以进行验证。
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引用次数: 0
Enhancing Sensitivity of Manganese Detection in Drinking Water Using Nanomaterial AuNPs/GP 纳米材料AuNPs/GP提高饮用水中锰的检测灵敏度
Pub Date : 2023-08-01 DOI: 10.1149/2754-2726/acec59
Kirsten Macdonald, Y. Pei, Adekunle Omoboye, Nicholas Lamothe, Yichun Shi, Kevin McEleney, S. J. Payne, Zhe She
Manganese (Mn) was previously considered a mere aesthetic concern that causes colored water and stained surfaces; however, recent epidemiological research found that excessive exposure to Mn has neurotoxic effects on humans, especially in children. In response to the health concerns, Health Canada and the World Health Organization moved towards stricter standards on Mn to protect public health. Currently, the standard analytical methods for Mn2+ are spectroscopic. Although they are highly sensitive, they are not cost effective or portable for high frequency analysis in the field. In this article, the sensitivity of electrochemical techniques, chronoamperometry (CA) and cathodic stripping voltammetry (CSV), are compared as well as the sensitivity of a non-modified glassy carbon screen-printed electrode (GCE SPE) vs a gold nanoparticle modified graphene (AuNPs/GP) coated GCE SPE for Mn2+ detection and quantification. Regarding the coating of the GCE SPE, detection performed with AuNPs/GP modified GCE SPE shows a wider linear range from 0–520 μM and an improved LOD of 0.75 μM. Application of the sensors was tested using drinking water samples returning high recovery rates from 92.9 to 106.8% depending on material and method used for Mn2+ detection and quantification.
锰(Mn)以前被认为只是一种美学问题,会导致水着色和表面污染;然而,最近的流行病学研究发现,过量接触锰对人类有神经毒性影响,尤其是对儿童。为了应对健康问题,加拿大卫生部和世界卫生组织采取了更严格的锰标准,以保护公众健康。目前,Mn2+的标准分析方法是光谱法。尽管它们是高度敏感的,但对于该领域的高频分析来说,它们不是成本有效的或便携式的。本文比较了电化学技术的灵敏度,计时电流法(CA)和阴极溶出伏安法(CSV),以及非修饰玻碳丝网印刷电极(GCE SPE)与金纳米粒子修饰石墨烯(AuNPs/GP)涂层的GCE SPE对Mn2+检测和定量的灵敏度。关于GCE SPE的涂层,用AuNPs/GP修饰的GCE SPE进行的检测显示出0–520μM的更宽线性范围和0.75μM的改进LOD。使用饮用水样品测试了传感器的应用,根据用于Mn2+检测和定量的材料和方法,返回了92.9%至106.8%的高回收率。
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引用次数: 0
L-Alanine Supported Autogenous Eruption Combustion Synthesis of Ni/NiO@RuO2 Heterostructure for Electrochemical Glucose and pH Sensor l -丙氨酸负载的自喷发燃烧合成Ni/NiO@RuO2异质结构电化学葡萄糖和pH传感器
Pub Date : 2023-07-21 DOI: 10.1149/2754-2726/ace983
N. Padmanathan, R. Sasikumar, V. Thayanithi, K. Razeeb
Safety and quality control are important for long-term storage and preservation of food. Glucose and food pH are the two most common markers for evaluating food quality. Herein, we constructed a Ni/NiO@RuO2 heterostructure-based two-way sensor via a novel eruption combustion pattern (ECP) using non-conventional amino acid as a propellant. This approach has the unique points of interests of in situ doping of oxides and the formation of heterojunctions, providing well-developed pores and high surface areas to enhance the material performance. The Ni/NiO@RuO2 heterostructures have been tested as a bi-functional catalyst for glucose and pH sensing. The sensor exhibits a fast response time of <0.1 ± 0.02 s, a sensitivity of 641.95 ± 0.5 μA mM−1 cm−2 towards glucose with a 0.4 ± 0.08 μM detection limit and a linear response of 0.1 to 5 mM. As a pH sensor, it exhibits an acceptable sensitivity of −41.6 mV pH−1 with a response time of <50 s over a pH range of 2–12. Moreover, this bi-functional sensor based on Ni/NiO@RuO2 performs well when applied to a selection of beverage samples. This study provides a new scalable and low-cost approach to fabricating hetero-oxide nanostructures with controllable heterojunctions for various sensor applications.
安全和质量控制对于食品的长期储存和保存非常重要。葡萄糖和食品pH值是评价食品质量的两个最常见的指标。在这里,我们构建了一个基于Ni/NiO@RuO2异质结构的双向传感器,通过一种新的喷发燃烧模式(ECP),使用非常规氨基酸作为推进剂。该方法具有氧化物原位掺杂和异质结形成的独特兴趣点,提供了发育良好的孔隙和高表面积,以提高材料的性能。Ni/NiO@RuO2异质结构已被测试为葡萄糖和pH感应的双功能催化剂。该传感器对葡萄糖的响应时间<0.1±0.02 s,灵敏度为641.95±0.5 μA mM−1 cm−2,检测限为0.4±0.08 μM,线性响应范围为0.1 ~ 5mm。作为pH传感器,在2 ~ 12的pH范围内,灵敏度为- 41.6 mV pH−1,响应时间<50 s。此外,这种基于Ni/NiO@RuO2的双功能传感器在应用于选定的饮料样品时表现良好。该研究提供了一种新的可扩展和低成本的方法来制造具有可控异质结的异质氧化物纳米结构,用于各种传感器应用。
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引用次数: 1
Review—Reaction-Based Microcantilever Sensors 综述——基于反应的微悬臂梁传感器
Pub Date : 2023-07-21 DOI: 10.1149/2754-2726/ace982
Y. Lam, Disha Patel, Ariel Vaknin, Lee W. Hoffman, T. Thundat, Hai-Feng Ji
Microcantilevers (MCLs) have proven to be a cost-effective, label-free, and portable analytical technique for the detection of chemical and biological species. The MCL method offers significant benefits, primarily owing to its high sensitivity, which enables the detection of cantilever motion with sub-nanometer precision. Additionally, this method is well-suited for fabrication into a multi-element sensor array, further enhancing its capabilities. Most of the sensors are based on adsorption-induced frequency or surface stress changes of MCLs. Multiple review articles on this concept have been published, but no review has been published summarizing the MCL sensors with a focus on reactions. Other than detecting chemical species, another unique application of MCLs is their ability to characterize the morphology and mechanical properties of materials on a solid-liquid or solid-gas interface during a reaction process. We will review the reaction-based MCL sensors and also their potential applications in monitoring reactions in this short review article.
微抗体(MCL)已被证明是一种成本效益高、无标签、便携式的化学和生物物种检测分析技术。MCL方法具有显著的优势,主要是由于其高灵敏度,能够以亚纳米精度检测悬臂运动。此外,这种方法非常适合制造成多元件传感器阵列,进一步增强了其能力。大多数传感器基于MCL的吸附诱导频率或表面应力变化。已经发表了多篇关于这一概念的综述文章,但没有发表任何综述来总结MCL传感器,重点是反应。除了检测化学物质外,MCL的另一个独特应用是它们能够在反应过程中表征固液或固气界面上材料的形态和机械性能。在这篇简短的综述文章中,我们将回顾基于反应的MCL传感器及其在监测反应中的潜在应用。
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引用次数: 0
Exosomes, the Key Players in Cell-To-Cell Communication as the Universal Nano-Sized Disease Sensors of the Future 外泌体是细胞间通信的关键参与者,是未来的通用纳米级疾病传感器
Pub Date : 2023-07-21 DOI: 10.1149/2754-2726/ace981
K. Mohammadi, S. Badilescu, Anirban Ghosh, M. Packirisamy
Exosomes are nanovesicles shed by cells into the extracellular space. From an engineering point of view, exosomes can be assimilated to a complex system, that is, an arrangement of parts that together exhibit a behavior that the individual constituents do not. The authors argue that the unique composition of exosomes as well as their involvement in cell-to-cell communication and monitoring a multitude of diseases, justify entirely looking at exosomes, in the future, as universal sensors of diseases. In this paper, after the short background information on exosomes, the authors show how they are involved in the progress of different diseases.
外泌体是由细胞脱落到细胞外空间的纳米囊泡。从工程学的角度来看,外泌体可以被同化成一个复杂的系统,也就是说,一个部分的排列,共同表现出个体成分所没有的行为。作者认为,外泌体的独特组成以及它们参与细胞间通信和监测多种疾病,证明在未来外泌体完全可以作为疾病的通用传感器。在本文中,在简要介绍外泌体的背景信息后,作者展示了它们如何参与不同疾病的进展。
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引用次数: 0
Engineering Biosensors and Biomedical Detection Devices from 3D-Printed Technology 来自3d打印技术的工程生物传感器和生物医学检测设备
Pub Date : 2023-07-10 DOI: 10.1149/2754-2726/ace5bd
Minghui Liang, Xiang Liu, Yuying Chong, Ziyun Ye, Lei Zhao, Qiang Yu, Kai Tang, Anqi Geng, Bo Hu, Guanqun Ge, Shifang Yuan
Limitation of 3D construction ability, complex preparation processes and developing customer demands have promoted people to find low-cost, rapid prototyping, and simple operation methods to produce novel functional devices in the near future. Among various techniques, 3D-printed technology is a promising candidate for the fabrication of biosensors and biomedical detection devices with a wide variety of potential applications. This review offers four important 3D printing techniques for biosensors and biomedical detection devices and their applications. The principle and printing process of 3D-printed technologies will be generalized, and the printing performance of many 3D printers will be compared. Despite the resolution restrictions of 3D-printed, these technologies have already shown promising applications in many biosensors and biomedical detection devices, such as 3D-printed microfluidic devices, 3D-printed optical devices, 3D-printed electrochemical devices, and 3D-printed integrated devices. Some of the most representative examples will also be discussed here, demonstrating that 3D-printed technology can rationally design biosensors and biomedical detection devices and achieve important applications in microfluidic, optical, electrochemical, and integrated devices.
3D构建能力的局限性、复杂的制备过程和不断发展的客户需求促使人们在不久的将来找到低成本、快速成型和简单操作的方法来生产新型功能器件。在各种技术中,3D打印技术是制造生物传感器和生物医学检测设备的一种很有前途的候选技术,具有广泛的潜在应用。这篇综述提供了四种重要的生物传感器和生物医学检测设备的3D打印技术及其应用。将概括3D打印技术的原理和打印过程,并比较许多3D打印机的打印性能。尽管3D打印的分辨率受到限制,但这些技术已经在许多生物传感器和生物医学检测设备中显示出了很有前景的应用,例如3D打印的微流体设备、3D打印的光学设备、3D印刷的电化学设备和3D打印的集成设备。这里还将讨论一些最具代表性的例子,证明3D打印技术可以合理设计生物传感器和生物医学检测设备,并在微流体、光学、电化学和集成设备中实现重要应用。
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引用次数: 0
Editors’ Choice—Luminescent Oxygen Sensors: Valuable Tools for Spatiotemporal Exploration of Metabolism in In Vitro Systems 编辑的选择-发光氧传感器:在体外系统代谢的时空探索有价值的工具
Pub Date : 2023-07-05 DOI: 10.1149/2754-2726/ace202
Tyler Z Sodia, K. Cash
A common biological theme on Earth is the importance of oxygen, regardless of an organism’s metabolic capabilities. This commonality makes the quantification of O2 essential in understanding life as we know it. There are many sensing methods that enable researchers to measure this important analyte, but not all sensors are compatible with every system. This perspective highlights common O2 sensing formats (and recent innovations) with the goal of guiding the reader towards a sensor choice for their desired application. We emphasize the importance of exploring unfamiliar metabolic processes, commercializing new sensors, and establishing collaborations for maximizing innovation and accelerating discovery.
地球上一个常见的生物学主题是氧气的重要性,而与生物体的代谢能力无关。这种共性使得O2的量化对于理解我们所知的生命至关重要。有许多传感方法使研究人员能够测量这种重要的分析物,但并非所有传感器都与每个系统兼容。这一观点强调了常见的O2传感格式(以及最近的创新),目的是引导读者选择适合其所需应用的传感器。我们强调探索不熟悉的代谢过程、将新传感器商业化以及建立合作关系以最大限度地提高创新和加速发现的重要性。
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引用次数: 0
Cephalopods as a Natural Sensor-Display Feedback System Inspiring Adaptive Technologies 头足类作为一种自然传感器显示反馈系统的自适应技术
Pub Date : 2023-06-01 DOI: 10.1149/2754-2726/acdbdc
Lasanthi Sumathirathne, Taehwan Kim, Duncan Q. Bower, L. Deravi
Nature is full of exemplary species that have evolved personalized sensors and actuating systems that interface with and adapt to the world around them. Among them, cephalopods are unique. They employ fast-sensing systems that trigger structural changes to impart color changes through biochemical and optoelectronic controls. These changes occur using specialized optical organs that receive and respond to signals (light, temperature, fragrances, sound, and textures) in their environments. We describe features that enable these functions, highlight engineered systems that mimic them, and discuss strategies to consider for future cephalopod-inspired sensor technologies.
自然界充满了典型的物种,它们进化出了个性化的传感器和驱动系统,与周围的世界对接并适应。其中头足类动物是独一无二的。他们采用快速传感系统,通过生物化学和光电控制来触发结构变化,从而赋予颜色变化。这些变化是通过特殊的光学器官发生的,这些器官接收并响应环境中的信号(光、温度、香味、声音和纹理)。我们描述了实现这些功能的特征,强调了模仿它们的工程系统,并讨论了未来头足类传感器技术的考虑策略。
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引用次数: 0
Review—Environmental Biosensors for Agro-Safety Based on Electrochemical Sensing Mechanism with an Emphasis on Pesticide Screening 综述——以农药筛选为重点的基于电化学传感机制的农业安全环境生物传感器
Pub Date : 2023-06-01 DOI: 10.1149/2754-2726/acde5d
V. N. Dhamu, Durgasha C. Poudyal, Manish Samson, A. Paul, S. Muthukumar, Shalini Prasad
Climate change directly affects all aspects of the environment and accounts for changes in our surroundings seen or unseen. With the growing need for accountability in the agricultural commercial and regulatory spheres, there is a widely accepted consensus that there needs to be quantitative information on the different environmental entities to understand and solve challenges posed towards food production and agricultural activities. The main purpose of the creation of this article is to propagate an era of quantitative metrics to better assess environmental health which can adversely impact human health. This review assesses 3 different environmental vectors prone to pollution and in high contact with human entities. This review also discusses different electrochemical sensing platforms used as biosensors for the detection of a wide myriad of pollutants with an adverse effect on human health. Furthermore, the breakdown of each section includes a survey of the evolution and design of various sensor platforms that are aimed towards a comprehensive monitoring platform for pollutant screening with an emphasis on agro-safety and environmental health. Detailed pesticide screening case-studies are also explored for a better understanding of the current electrochemical biosensors that exist for the sole purpose of environmental monitoring.
气候变化直接影响到环境的各个方面,并解释了我们周围可见或不可见的变化。随着对农业、商业和监管领域问责制的日益需要,人们普遍认为,需要有关于不同环境实体的定量信息,以了解和解决对粮食生产和农业活动构成的挑战。创建这篇文章的主要目的是宣传一个量化指标的时代,以更好地评估可能对人类健康产生不利影响的环境健康。本综述评估了3种不同的易受污染和与人类实体高度接触的环境媒介。这篇综述还讨论了不同的电化学传感平台作为生物传感器,用于检测对人类健康有不利影响的各种污染物。此外,每一节的细分还包括对各种传感器平台的演变和设计的调查,这些传感器平台旨在建立一个综合监测平台,以筛选污染物,重点是农业安全和环境健康。还探讨了详细的农药筛选案例研究,以便更好地了解目前仅用于环境监测的电化学生物传感器。
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引用次数: 1
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