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Perspective-The Feasibility of Continuous Protein Monitoring in Interstitial Fluid. 透视间质液中蛋白质连续监测的可行性。
Pub Date : 2023-06-01 Epub Date: 2023-04-28 DOI: 10.1149/2754-2726/accd7e
Thomas Young, Vincent Clark, Netzahualcóyotl Arroyo-Currás, Jason Heikenfeld

Real-time continuous monitoring of proteins in-vivo holds great potential for personalized medical applications. Unfortunately, a prominent knowledge gap exists in the fundamental biology regarding protein transfer and correlation between interstitial fluid and blood. Additionally, technological sensing will require affinity-based platforms that cannot be robustly protected in-vivo and will therefore be challenged in sensitivity, longevity, and fouling over multi-day to week timelines. Here we use electrochemical aptamer sensors as a model system to discuss further research necessary to achieve continuous protein sensing.

体内蛋白质的实时连续监测在个性化医疗应用方面具有巨大潜力。不幸的是,在蛋白质转移以及间质液和血液之间的相关性方面,基础生物学存在着显著的知识差距。此外,技术传感将需要基于亲和力的平台,这些平台无法在体内得到强有力的保护,因此在几天到几周的时间内,在灵敏度、寿命和污垢方面将受到挑战。在这里,我们使用电化学适体传感器作为模型系统来讨论实现连续蛋白质传感所需的进一步研究。
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引用次数: 1
Review—Basic and Advanced Inertial Navigation Fluid-Based Technology 综述-基于流体的基本和先进惯性导航技术
Pub Date : 2023-04-27 DOI: 10.1149/2754-2726/acd0c0
R. Elaswad, N. El-Sheimy, A. A. Mohamad
The article reviews most published inertial sensor technologies, including dynamically tuned, optical, MEMS vibratory, mechanical, solid-state, and fluid-based. The working principles of the technologies are elaborated. Also, the advantages and disadvantages of those sensors are laid out. Owing to its excellent overall performance, such as its simple structure, low cost, large measurement range, etc, the current review focuses on the state-of-the-art of fluid-based technology of accelerometers and gyroscopes. The sensing elements of the fluid-based technology that are used in the accelerometer and gyroscope are explained. Moreover, a comparison and analysis of those sensing elements are presented. The comparison shows that the thermal resistor has five orders of magnitude which is the highest dynamic range. However, the porous transducer is higher in bandwidth which is almost 120 Hz. Furthermore, the particle imaging velocimetry gyroscope (PIVG) is reviewed. The PIVG is an innovative technology that is used to measure the angular rate where fluid is used as proof of mass. The review shows that the PIVG is low-cost and almost drift-free. Additionally, compared to commercially available gyroscopes, PIVG provides a superior signal-to-noise ratio (SNR).
本文回顾了大多数已发表的惯性传感器技术,包括动态调谐、光学、MEMS振动、机械、固态和基于流体的。阐述了这些技术的工作原理。同时,分析了这些传感器的优缺点。基于流体技术的加速度计和陀螺仪具有结构简单、成本低、测量范围大等综合性能优异的特点,本文主要介绍流体技术在加速度计和陀螺仪中的研究进展。介绍了加速度计和陀螺仪中采用的基于流体技术的传感元件。此外,还对这些传感元件进行了比较和分析。对比表明,该热敏电阻具有5个数量级的最高动态范围。然而,多孔换能器的带宽更高,几乎为120 Hz。此外,还对粒子成像测速陀螺仪(PIVG)进行了综述。PIVG是一项创新技术,用于测量流体用作质量证明的角速率。回顾表明,PIVG是低成本的,几乎无漂移。此外,与市售陀螺仪相比,PIVG提供了更高的信噪比(SNR)。
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引用次数: 0
Watch-Type Dual-Mode Wearable Health Device 手表型双模可穿戴健康设备
Pub Date : 2023-03-24 DOI: 10.1149/2754-2726/acc74d
Hyusim Park, Liem H. T. Nguyen, Shanthala Lakshminarayana, Yuze Sun, Sungyong Jung
Wearable devices can be found as numerous types of health devices ranging from monitoring hazardous environments to detecting physiological signals. Its utilities have also shown encouraging improvements toward personalized healthcare. Thus, many kinds of wearable health devices (WHD) have been reported and developed. Those reports highlight their efficiency in monitoring harmful environmental factors and various diseases for diagnostic and treatment purposes. This paper proposes a watch-type of dual-mode WHD that can perform gaseous phase detection to monitor dangerous environmental situations such as bad air quality and aqueous phase detection to measure physiological signals from human sweat or blood. The proposed system was fabricated on a printed circuit board and its size is 3 cm × 3 cm which is suitable as a wearable device. It also consumes very low power and has capabilities of wired/wireless charging and wired/wireless communication. Furthermore, customized windows and Android applications have been developed to visualize obtained data in a user’s computer or smartphone. The system was tested both electrically and chemically and showed promising results as the WHD.
可穿戴设备可以作为多种类型的健康设备,从监测危险环境到检测生理信号。它的公用事业也在个性化医疗方面取得了令人鼓舞的进步。因此,许多类型的可穿戴健康设备(WHD)被报道和开发。这些报告强调了它们在监测有害环境因素和为诊断和治疗目的监测各种疾病方面的效率。本文提出了一种手表式的双模式WHD,它可以进行气相检测来监测恶劣空气质量等危险环境情况,也可以进行水相检测来测量人体汗液或血液的生理信号。该系统在印刷电路板上制作,尺寸为3cm × 3cm,适合作为可穿戴设备。它还消耗非常低的功率,并具有有线/无线充电和有线/无线通信的能力。此外,定制的窗口和Android应用程序已经开发出来,可以在用户的计算机或智能手机中可视化获得的数据。该系统进行了电气和化学测试,并显示出令人满意的结果。
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引用次数: 2
Modified Fe3O4 Magnetite Core@Shell Type Nanomaterials for Highly-Responsive LPG Sensing: A Comparative Analysis 用于高响应LPG传感的改性Fe3O4磁铁矿Core@Shell型纳米材料的比较分析
Pub Date : 2023-03-13 DOI: 10.1149/2754-2726/acc3ab
Shakti Singh, A. Dzeranov, L. Bondarenko, K. Kydralieva, G. Dzhardimalieva, A. Babaytsev, G. Kugabaeva, N. Golubeva, B. Yadav
The present work focuses on the synthesis of Fe3O4 magnetite core@shell type nanoparticles modified with three types of ligands: Magnetite with activated carbon (MAC), Magnetite with silica (tetraethoxysilane, TEOS, and 3-aminopropyltriethoxysilane, APTES) (MTA) and Magnetite with silica, APTES and humic acids (MTAH). The MTAH sample shows greater porosity in comparison to MTA, and MAC samples. The band gap of MTAH is 4.08 eV, which is higher than MTA (2.92 eV), and MAC (2.80 eV). Rietveld quantitative phase analysis of all derivatives was performed and compared with all three samples. The LPG sensing at room temperature shows the highest sensor response of 9.42, in comparison to 3.87 sensor response for MAC, and 4.60 for MTA. This approximately double sensor response increment is justified with the help of band gap, porosity, and size of all 3 the samples. The MTAH sample shows the lowest response-recovery time of 9.33 and 10.78 s respectively in comparison to MAC and MTA samples. In conclusion, this manuscript describes the synthesis procedure of different derivatives of Fe3O4 core@shell materials along with the relation of LPG sensing with different parameters of the materials.
本文主要研究了Fe3O4磁铁矿的合成core@shell用三种类型的配体修饰的类型纳米颗粒:磁铁矿与活性炭(MAC)、磁铁矿与二氧化硅(四乙氧基硅烷、TEOS和3-氨基丙基三乙氧基硅烷,APTES)(MTA)和磁铁矿与二氧化硅、APTES和腐殖酸(MTAH)。与MTA和MAC样品相比,MTAH样品显示出更大的孔隙率。MTAH的带隙为4.08eV,高于MTA(2.92eV)和MAC(2.80eV)。对所有衍生物进行了Rietveld定量相分析,并与所有三个样品进行了比较。室温下的LPG传感显示出9.42的最高传感器响应,相比之下,MAC和MTA的传感器响应分别为3.87和4.60。在所有3个样品的带隙、孔隙率和尺寸的帮助下,这种近似两倍的传感器响应增量是合理的。与MAC和MTA样本相比,MTAH样本显示出最低的响应恢复时间,分别为9.33和10.78秒。总之,本文描述了Fe3O4不同衍生物的合成过程core@shell以及LPG传感与不同材料参数的关系。
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引用次数: 2
Progression in Quantum Sensing/Bio-Sensing Technologies for Healthcare 用于医疗保健的量子传感/生物传感技术进展
Pub Date : 2023-03-06 DOI: 10.1149/2754-2726/acc190
Vibhas Chugh, Adreeja Basu, A. Kaushik, A. Basu
The 5th/6th generation bio-sensing technology is an emerging field which connects smart technologies like Artificial Intelligence, Internet of Things and Machine Learning with efficient micro/nano-enabled sensing platform for making point-of-care (POC) devices to investigate health management strategies. Recently, the integration and interfacing between quantum measurement, signaling, and optimized bio-actives has led to investigate the minute biological events with anomalous sensitivity. Such technologies are expected to provide the possibility to measure and record changes at quantum scales with varying pressure, temperature, and electromagnetic fields. Considering current scenarios, this perspective critically highlights state-of-art quantum sensing technology along with their challenges and prospects.
第5/6代生物传感技术是一个新兴领域,它将人工智能、物联网和机器学习等智能技术与高效的微/纳米传感平台连接起来,用于制造护理点(POC)设备,以研究健康管理策略。最近,量子测量、信号传导和优化的生物活性物质之间的集成和接口导致了对具有异常灵敏度的微小生物事件的研究。这些技术有望提供在不同压力、温度和电磁场的量子尺度上测量和记录变化的可能性。考虑到当前的场景,这一观点批判性地突出了量子传感技术的现状及其挑战和前景。
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引用次数: 10
Expanding the Monolayer Scope for Nucleic Acid-Based Electrochemical Sensors Beyond Thiols on Gold: Alkylphosphonic Acids on ITO. 扩大基于核酸的电化学传感器的单层范围,超越金上的硫醇:ITO上的烷基膦酸。
Pub Date : 2023-03-01 Epub Date: 2023-03-29 DOI: 10.1149/2754-2726/acc4d9
Alexander Shaver, Netzahualcóyotl Arroyo-Currás

Electrochemical biosensors are a powerful and rapidly evolving molecular monitoring technology. Evidenced by the success of the continuous glucose monitor in managing Type 1 Diabetes, these sensors are capable of precise, accurate measurements in unprocessed biological environments. Nucleic acid-based electrochemical sensors (NBEs) are a specific type of biosensor that employs the target binding and conformational dynamics of nucleic acids for signal transduction. Currently, the vast majority of NBEs are fabricated via self-assembly of alkylthiols on Au electrodes. However, this architecture is limited in scope, as Au electrodes are not universally deployable for all potential NBE applications. Here, to expand the repertoire of materials on which NBEs can be made, we describe the multistep procedure for creating sensing monolayers of alkylphosphonic acids on a conductive oxide surface. Using such monolayers on indium tin oxide (ITO)-coated glass slides, we couple redox reporter-modified nucleic acids and demonstrate signaling of procaine-binding NBE sensors in buffer and human serum. We investigate the operational stability of these NBE sensors to reveal faster signal loss relative to benchmark thiol-on-gold sensing layers, a result that arises due to poor stability of the underlying ITO. Finally, we discuss future directions to continue expansion of NBE sensor materials and applications.

电化学生物传感器是一种强大且快速发展的分子监测技术。连续血糖监测仪在治疗1型糖尿病方面的成功证明,这些传感器能够在未经处理的生物环境中进行精确、准确的测量。基于核酸的电化学传感器(NBE)是一种特殊类型的生物传感器,它利用核酸的靶标结合和构象动力学进行信号转导。目前,绝大多数NBE是通过烷基硫醇在Au电极上的自组装制备的。然而,这种结构的范围是有限的,因为Au电极并不能普遍用于所有潜在的NBE应用。在这里,为了扩大可以制造NBE的材料库,我们描述了在导电氧化物表面上创建烷基膦酸传感单层的多步骤程序。在涂有氧化铟锡(ITO)的载玻片上使用这种单层,我们偶联了氧化还原报告基因修饰的核酸,并证明了缓冲液和人血清中普鲁卡因结合NBE传感器的信号传导。我们研究了这些NBE传感器的操作稳定性,以揭示相对于金传感层上的基准硫醇更快的信号损失,这是由于底层ITO的稳定性差而产生的结果。最后,我们讨论了NBE传感器材料和应用的未来发展方向。
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引用次数: 0
Comparison of Machine Learning Algorithms for Natural Gas Identification with Mixed Potential Electrochemical Sensor Arrays 混合电位电化学传感器阵列识别天然气的机器学习算法比较
Pub Date : 2023-02-22 DOI: 10.1149/2754-2726/acbe0c
Neal Ma, Sleight Halley, K. Ramaiyan, F. Garzon, L. Tsui
Mixed-potential electrochemical sensor arrays consisting of indium tin oxide (ITO), La0.87Sr0.13CrO3, Au, and Pt electrodes can detect the leaks from natural gas infrastructure. Algorithms are needed to correctly identify natural gas sources from background natural and anthropogenic sources such as wetlands or agriculture. We report for the first time a comparison of several machine learning methods for mixture identification in the context of natural gas emissions monitoring by mixed potential sensor arrays. Random Forest, Artificial Neural Network, and Nearest Neighbor methods successfully classified air mixtures containing only CH4, two types of natural gas simulants, and CH4+NH3 with >98% identification accuracy. The model complexity of these methods were optimized and the degree of robustness against overfitting was determined. Finally, these methods are benchmarked on both desktop PC and single-board computer hardware to simulate their application in a portable internet-of-things sensor package. The combined results show that the random forest method is the preferred method for mixture identification with its high accuracy (>98%), robustness against overfitting with increasing model complexity, and had less than 10 ms training time and less than 0.1 ms inference time on single-board computer hardware.
由氧化铟锡(ITO)、La0.87Sr0.13CrO3、Au和Pt电极组成的混合电位电化学传感器阵列可以检测天然气基础设施的泄漏。需要算法从湿地或农业等背景自然和人为来源中正确识别天然气来源。我们首次报道了在混合电位传感器阵列监测天然气排放的背景下,几种用于混合物识别的机器学习方法的比较。随机森林、人工神经网络和最近邻方法成功地对仅含有CH4、两种类型的天然气模拟物和CH4+NH3的空气混合物进行了分类,识别准确率>98%。对这些方法的模型复杂度进行了优化,并确定了对过拟合的鲁棒性程度。最后,在台式PC和单板计算机硬件上对这些方法进行了测试,以模拟它们在便携式物联网传感器包中的应用。综合结果表明,随机森林方法是混合识别的首选方法,其精度高(>98%),对模型复杂度增加的过拟合具有鲁棒性,在单板计算机硬件上的训练时间小于10ms,推理时间小于0.1ms。
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引用次数: 1
The Era of Biosensors and Diagnostics 生物传感器和诊断的时代
Pub Date : 2023-01-23 DOI: 10.1149/2754-2726/acb3de
N. Arroyo-Currás
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引用次数: 0
AI Enabled Ensemble Deep Learning Method for Automated Sensing and Quantification of DNA Damage in Comet Assay 人工智能集成深度学习方法用于彗星检测中DNA损伤的自动传感和量化
Pub Date : 2023-01-13 DOI: 10.1149/2754-2726/acb2da
Prateek Mehta, Srikanth Namuduri, L. Barbé, Stephanie Lam, Zohreh Faghihmonzavi, Vivek Kamat, S. Finkbeiner, S. Bhansali
Comet assay is a widely used technique to assess and quantify DNA damage in individual cells. Recently, researchers have applied various deep learning techniques to automate the analysis of comet assay. Image analysis using deep learning allows combining multiple parameters of images and performing computation at a pixel level to provide quantifiable information about the comets. The current deep learning analysis algorithms use a single neural network as a standard method, which relies on many comet images and prone to high variance in predictions. Here, we propose a new ensemble model consisting of a collection of deep learning networks with different configurations and different initial random weights trained on the same dataset to calculate one weighted prediction for DNA damage quantification. To develop this model, we curated a trainable comet assay image dataset consisting of1309 images with 9204 extracted features of cell head and tail length, area, etc With the proposed method we could achieve significantly higher accuracy (R2 = 89.3%, compared to 74% with the standard single neural network as reported in data published by M. D. Zeiler and R Fergus (European conference on computer vision, pp. 818–833 2014). Furthermore, deep regression with the proposed architecture produced much more reliable and accurate results than conventional method.
彗星试验是一种广泛使用的技术,用于评估和量化单个细胞中的DNA损伤。最近,研究人员应用了各种深度学习技术来自动化彗星分析。使用深度学习的图像分析允许组合图像的多个参数,并在像素级别执行计算,以提供有关彗星的可量化信息。目前的深度学习分析算法使用单个神经网络作为标准方法,该方法依赖于许多彗星图像,并且预测中容易出现高方差。在这里,我们提出了一个新的集成模型,该模型由一组具有不同配置和在同一数据集上训练的不同初始随机权重的深度学习网络组成,以计算DNA损伤量化的一次加权预测。为了开发这个模型,我们策划了一个可训练的彗星分析图像数据集,该数据集由1309张图像组成,其中9204张提取了细胞头部和尾部长度、面积等特征。根据M.D。Zeiler和R Fergus(欧洲计算机视觉会议,2014年,第818–833页)。此外,与传统方法相比,采用所提出的架构的深度回归产生了更可靠和准确的结果。
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引用次数: 1
Perspective—Supercapacitor-Powered Flexible Wearable Strain Sensors 透视-超级电容器供电的柔性可穿戴应变传感器
Pub Date : 2023-01-12 DOI: 10.1149/2754-2726/acb27a
C. Manjunatha, K. Shwetha, Y. Athreya, S. G. Kumar, M. K. S. Kamath
Currently and also in future, the flexible and wearable strain sensor would be in high demand due to its direct applications in biomedical health monitoring and other engineering applications. The challenge is to make the flexible and wearable sensor to function continuously with no additional wired powered supply system. In line with this, there has been considerable research works towards the integration of supercapacitor into flexible and wearable strain sensors, to make them self-powered and more efficient. This perspective provides more insights on design and fabrication of flexible and wearable strain sensor, working, components, and materials used, integration with supercapacitor, challenges and future scope.
柔性、可穿戴应变传感器直接应用于生物医学、健康监测等工程领域,在当前和未来都将受到广泛的需求。挑战在于使柔性和可穿戴传感器在没有额外的有线供电系统的情况下连续工作。与此相一致,已经有相当多的研究工作将超级电容器集成到柔性和可穿戴的应变传感器中,使其自供电和更高效。这一观点提供了更多关于柔性和可穿戴应变传感器的设计和制造、工作、组件和使用的材料、与超级电容器的集成、挑战和未来范围的见解。
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引用次数: 7
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