Sagar R Barge, Anupam Bhattacharya, Arun Kumar, Sushmita Das, Tulsi Joishy, Ashis K Mukherjee, Maloyjo Joyraj Bhattacharjee, Mojibur R Khan
Functional foods, such as fermented bamboo shoots, have a long history of consumption among the ethnic communities in northeast India. These locally fermented bamboo shoots contain a wealth of beneficial microbes and metabolites that can help combat metabolic syndromes like obesity. However, the precise effects and mechanism behind fermented bamboo shoot products and their anti-obesity properties remain unknown. This study aims to explore the different types of fermented bamboo shoot products to determine their potential anti-obesity effects as well as to analyze their microbial diversity and metabolite profiles. Using both culture-dependent and culture-independent methods, we found a high abundance of lactic acid bacteria from the Firmicutes and Proteobacteria phyla in the sample. Gas chromatography–mass spectrometry (GC–MS) based untargeted metabolite profiling detected several aroma-active compounds, bioactive metabolites, short-chain fatty acids, and essential amino acids in the samples. The water extract derived from a particular type of fermented bamboo shoot, Melye-amiley, was found to significantly reduce intracellular lipid accumulation in cultured 3T3-L1 cells. In addition, this extract increased the expression of lipolytic (hormone-sensitive lipase, lipoprotein lipase, and adipose triglyceride lipase) and browning regulator genes (uncoupling protein [UCP1], PRDM16, and PGC1-alpha). By activating the AMPK signaling pathway, the water extract from Melye-amiley also upregulated thermogenic protein expression and promoted mitochondrial biogenesis and fatty acid β-oxidation. These findings suggest that fermented bamboo shoot extract has promising anti-obesity effects by boosting energy expenditure in white adipocytes. Future research is necessary to identify the active ingredient(s) that may lead to new therapies to treat obesity.
{"title":"A traditional fermented bamboo shoot reduces intracellular fat accumulation and promotes fat browning in differentiated 3T3-L1 adipocyte cells through the activation of the AMPK signaling pathway","authors":"Sagar R Barge, Anupam Bhattacharya, Arun Kumar, Sushmita Das, Tulsi Joishy, Ashis K Mukherjee, Maloyjo Joyraj Bhattacharjee, Mojibur R Khan","doi":"10.1002/fft2.405","DOIUrl":"10.1002/fft2.405","url":null,"abstract":"<p>Functional foods, such as fermented bamboo shoots, have a long history of consumption among the ethnic communities in northeast India. These locally fermented bamboo shoots contain a wealth of beneficial microbes and metabolites that can help combat metabolic syndromes like obesity. However, the precise effects and mechanism behind fermented bamboo shoot products and their anti-obesity properties remain unknown. This study aims to explore the different types of fermented bamboo shoot products to determine their potential anti-obesity effects as well as to analyze their microbial diversity and metabolite profiles. Using both culture-dependent and culture-independent methods, we found a high abundance of lactic acid bacteria from the Firmicutes and Proteobacteria phyla in the sample. Gas chromatography–mass spectrometry (GC–MS) based untargeted metabolite profiling detected several aroma-active compounds, bioactive metabolites, short-chain fatty acids, and essential amino acids in the samples. The water extract derived from a particular type of fermented bamboo shoot, <i>Melye-amiley</i>, was found to significantly reduce intracellular lipid accumulation in cultured 3T3-L1 cells. In addition, this extract increased the expression of lipolytic (hormone-sensitive lipase, lipoprotein lipase, and adipose triglyceride lipase) and browning regulator genes (uncoupling protein [<i>UCP1</i>]<i>, PRDM16</i>, and <i>PGC1-alpha</i>). By activating the AMPK signaling pathway, the water extract from <i>Melye-amiley</i> also upregulated thermogenic protein expression and promoted mitochondrial biogenesis and fatty acid β-oxidation. These findings suggest that fermented bamboo shoot extract has promising anti-obesity effects by boosting energy expenditure in white adipocytes. Future research is necessary to identify the active ingredient(s) that may lead to new therapies to treat obesity.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.405","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141020867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Diksha Chadha, Nazimah Hamid, Nolwenn Poulain, Kevin Kantono, R. Janani, Pey Sze Teo
The study explored the sociocultural influences on sugar consumption in three distinct countries: New Zealand, France, and Singapore. It employed the theory of planned behavior (TPB) alongside consumer perception and acceptance factors, such as sugar consciousness, product labeling, sugar substitution with sweeteners, and perceived flavor, to investigate participants’ behaviors regarding various yogurt types. Data was gathered through web surveys in each country, analyzed through partial least squares (PLS)–confirmatory factor analysis to create country-specific conceptual frameworks, and then validated using PLS-path modeling to assess the correlations between TPB and consumer perception constructs. The results indicated that perceived behavioral control positively influenced intentions to reduce sugar intake in participants from New Zealand and Singapore. Sugar consciousness exhibited a positive correlation with behavioral intentions across all three countries, suggesting heightened awareness of sugar intake motivates individuals to cut back. Notably, the presence of labels and claims showed negative correlation with perceived flavor, among the participants from New Zealand and France, suggesting that food researchers must navigate a delicate balance between labels and flavor to successfully design and market sugar-reduced products. Further analysis, PLS-multigroup analysis revealed significant difference in the impact of subjective norms on attitude between participants from different countries. Singaporeans placed stronger value on others’ approval for consuming less sugar-sweetened yogurt, whereas French and New Zealand participants had a more neutral stance. This study's novelty lies in its comprehensive exploration of sociocultural factors, integration of TPB with consumer perception constructs, and tailoring of analysis to each country's cultural context, thereby enhancing our understanding of multicultural sugar consumption patterns.
{"title":"Beyond borders: A cross-cultural analysis of consumption and purchase behaviour of sweeteners in yoghurts","authors":"Diksha Chadha, Nazimah Hamid, Nolwenn Poulain, Kevin Kantono, R. Janani, Pey Sze Teo","doi":"10.1002/fft2.402","DOIUrl":"10.1002/fft2.402","url":null,"abstract":"<p>The study explored the sociocultural influences on sugar consumption in three distinct countries: New Zealand, France, and Singapore. It employed the theory of planned behavior (TPB) alongside consumer perception and acceptance factors, such as sugar consciousness, product labeling, sugar substitution with sweeteners, and perceived flavor, to investigate participants’ behaviors regarding various yogurt types. Data was gathered through web surveys in each country, analyzed through partial least squares (PLS)–confirmatory factor analysis to create country-specific conceptual frameworks, and then validated using PLS-path modeling to assess the correlations between TPB and consumer perception constructs. The results indicated that perceived behavioral control positively influenced intentions to reduce sugar intake in participants from New Zealand and Singapore. Sugar consciousness exhibited a positive correlation with behavioral intentions across all three countries, suggesting heightened awareness of sugar intake motivates individuals to cut back. Notably, the presence of labels and claims showed negative correlation with perceived flavor, among the participants from New Zealand and France, suggesting that food researchers must navigate a delicate balance between labels and flavor to successfully design and market sugar-reduced products. Further analysis, PLS-multigroup analysis revealed significant difference in the impact of subjective norms on attitude between participants from different countries. Singaporeans placed stronger value on others’ approval for consuming less sugar-sweetened yogurt, whereas French and New Zealand participants had a more neutral stance. This study's novelty lies in its comprehensive exploration of sociocultural factors, integration of TPB with consumer perception constructs, and tailoring of analysis to each country's cultural context, thereby enhancing our understanding of multicultural sugar consumption patterns.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.402","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141027378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
People with diabetes mellitus (DM) and hyperglycemia are linked with cancer risk. Diabetes and cancer have been corroborated by high morbidity and mortality rates. Studies revealed that elevated levels of insulin secretions trigger insulin-like growth factor 1 (IGF-1) production. Moreover, IGF-1 is a key regulator involved in promoting cancer cell progression and is linked with DM. Cancer drug resistance and ototoxic effects can adversely affect the health and lifespan of an individual. However, naturally derived bioactive compounds are gaining attention for their nontoxic properties and specific behavior. Likewise, probiotics have also been regarded as safe and successful alternatives to treat DM-linked cancers. The present review aims to highlight the therapeutic potential and feasible functions of probiotics to mitigate or inhibit DM-associated cancers. Meanwhile, the intracellular signaling cascades involved in promoting DM-linked cancer are enumerated for future prospective research. However, metabolomics interactions and protein–protein interactions are to be discussed for deeper insights into affirmative principles in diabetic-linked cancers. Drug discovery and innovative preclinical evaluation need further adjuvant and immune-enhancement therapies. Furthermore, the results of the in silico assessment could provide scientific excellence of IGF-1 in diabetes and cancer. Overall, this review summarizes the mechanistic insights and therapeutic targets for diabetes-associated cancer.
{"title":"Feasible mechanisms and therapeutic potential of food probiotics to mitigate diabetes-associated cancers: A comprehensive review and in silico validation","authors":"Annadurai Vinothkanna, Xiang Shi-Liang, Durairaj Karthick Rajan, Ragothaman Prathiviraj, Soundarapandian Sekar, Shubing Zhang, Bo Wang, Zhu Liu, Ai-Qun Jia","doi":"10.1002/fft2.406","DOIUrl":"10.1002/fft2.406","url":null,"abstract":"<p>People with diabetes mellitus (DM) and hyperglycemia are linked with cancer risk. Diabetes and cancer have been corroborated by high morbidity and mortality rates. Studies revealed that elevated levels of insulin secretions trigger insulin-like growth factor 1 (IGF-1) production. Moreover, IGF-1 is a key regulator involved in promoting cancer cell progression and is linked with DM. Cancer drug resistance and ototoxic effects can adversely affect the health and lifespan of an individual. However, naturally derived bioactive compounds are gaining attention for their nontoxic properties and specific behavior. Likewise, probiotics have also been regarded as safe and successful alternatives to treat DM-linked cancers. The present review aims to highlight the therapeutic potential and feasible functions of probiotics to mitigate or inhibit DM-associated cancers. Meanwhile, the intracellular signaling cascades involved in promoting DM-linked cancer are enumerated for future prospective research. However, metabolomics interactions and protein–protein interactions are to be discussed for deeper insights into affirmative principles in diabetic-linked cancers. Drug discovery and innovative preclinical evaluation need further adjuvant and immune-enhancement therapies. Furthermore, the results of the in silico assessment could provide scientific excellence of IGF-1 in diabetes and cancer. Overall, this review summarizes the mechanistic insights and therapeutic targets for diabetes-associated cancer.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.406","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141024643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Circadian rhythm disorder (CRD) is closely associated with hair regression and shedding, but whether this risk can be transmitted to the offspring is unknown. Whether Pu-erh tea, with alleviating effects of CRD-mediated syndrome, can act on the transmission of alopecia risk to offspring is also unproven. Here, we obtained CRD parental mice offspring and found that CRD-mediated alopecia risk can be transmitted to offspring, especially male offspring. Parental consumption of Pu-erh tea, especially in females or both parents, reduced the risk of CRD-mediated alopecia transmitted to offspring by inhibiting subcutaneous fat accumulation (downregulation of Rab18, fat-specific protein 27 (Fsp27), and perilipin 1 (Plin1)), reducing oxidative stress and inflammation in skin tissue (NADPH oxidase 4 (NOX4)/ nuclear factor kappa-B (NF-κB)), balancing androgen and hair growth factor release (hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), insulin-like growth factor 1 (IGF-1)), and restoring hair follicle DNA repair function (upregulation of Ku70, 8-Oxoguanine DNA glycosylase 1 (OGG1), and Rad51). Transcriptomic analysis further clarified that the mechanism stemmed from the upregulation of gene expression in pathways such as the Wnt, Hippo, and other signaling pathways.
昼夜节律紊乱(CRD)与毛发退行性脱落密切相关,但这种风险是否会遗传给后代尚不清楚。普洱茶具有缓解昼夜节律紊乱综合征的作用,是否能将脱发风险传递给后代也尚未得到证实。在此,我们获得了 CRD 亲本小鼠的后代,并发现 CRD 介导的脱发风险可传递给后代,尤其是雄性后代。通过抑制皮下脂肪堆积(Rab18、脂肪特异性蛋白27(Fsp27)和过脂素1(Plin1)的下调)、减少皮肤组织的氧化应激和炎症(NADPH氧化酶4(NOX4)/核因子卡巴-B(NF-κB)),父母(尤其是雌性或父母双方)饮用普洱茶可降低CRD介导的脱发遗传给后代的风险、平衡雄激素和毛发生长因子的释放(肝细胞生长因子(HGF)、血管内皮生长因子(VEGF)、胰岛素样生长因子 1(IGF-1)),恢复毛囊 DNA 修复功能(上调 Ku70、8-氧鸟嘌呤 DNA 糖基化酶 1(OGG1)和 Rad51)。转录组分析进一步明确了这一机制源于 Wnt、Hippo 和其他信号通路中基因表达的上调。
{"title":"Pu-erh tea reduces the transmission of CRD-mediated alopecia risk to offspring","authors":"Shanshan Hu, Jie Wang, Zhiyuan Lin, Bowen Zhang, Liyong Luo, Liang Zeng","doi":"10.1002/fft2.409","DOIUrl":"https://doi.org/10.1002/fft2.409","url":null,"abstract":"<p>Circadian rhythm disorder (CRD) is closely associated with hair regression and shedding, but whether this risk can be transmitted to the offspring is unknown. Whether Pu-erh tea, with alleviating effects of CRD-mediated syndrome, can act on the transmission of alopecia risk to offspring is also unproven. Here, we obtained CRD parental mice offspring and found that CRD-mediated alopecia risk can be transmitted to offspring, especially male offspring. Parental consumption of Pu-erh tea, especially in females or both parents, reduced the risk of CRD-mediated alopecia transmitted to offspring by inhibiting subcutaneous fat accumulation (downregulation of Rab18, fat-specific protein 27 (Fsp27), and perilipin 1 (Plin1)), reducing oxidative stress and inflammation in skin tissue (NADPH oxidase 4 (NOX4)/ nuclear factor kappa-B (NF-κB)), balancing androgen and hair growth factor release (hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), insulin-like growth factor 1 (IGF-1)), and restoring hair follicle DNA repair function (upregulation of Ku70, 8-Oxoguanine DNA glycosylase 1 (OGG1), and Rad51). Transcriptomic analysis further clarified that the mechanism stemmed from the upregulation of gene expression in pathways such as the Wnt, Hippo, and other signaling pathways.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.409","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141624200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jinrui Hu, Zhenpeng Li, Yan Sun, Wenyu Shi, Xiaoli Du, Shuang Meng, Zhengfei Yu, Jinyue Liu, Stephen Forsythe, Zhigang Cui, Haijian Zhou
Cronobacter sakazakii clonal complex 4 (CC4) is strongly associated with neonatal meningitis. However, C. sakazakii CC4-specific pathogenicity traits have not been determined. In this study, the comparative genomic analysis of 144 genomes of C. sakazakii CC4 strains and 376 non-CC4 C. sakazakii strains was undertaken. Twenty-four CC4 strains were previously undescribed clinical and nonclinical strains that had been collected from various regions in China (2006–2017). The remaining genomes were of multiple sequence types of C. sakazakii strains, which had been isolated worldwide (1973–2021). The pan-genome of C. sakazakii comprised 32,332 genes, of which 2.58% (832 genes) constituted the core genome. More C. sakazakii CC4 strains have a complete cusABCFR efflux system with a significant difference compared with non-CC4 strains, and the ibeB-homologous cusC gene in the cusABCFR efflux system is probably associated with the invasion of human brain microvascular endothelial cells (BMEC). In addition, the isolates with K2:CA2 capsule or type IV pili-associated genes associated with neonatal meningitis were statistically more present in CC4 strains than non-CC4 strains (p < .0001). The thorough description of VirB/VirD4 gene cluster for the type IV secretion system and the impB/impF and vasL/vasJ gene clusters for the type VI secretion system in C. sakazakii were provided. Overall, the specific and diverse virulence factors and genes may have led to C. sakazakii CC4 clone increasing its ability to invade human BMEC and leading to neonatal meningitis. Our findings should facilitate the development of novel strategies to prevent, diagnose, and treat C. sakazakii CC4 infections.
阪崎肠杆菌克隆复合体 4(CC4)与新生儿脑膜炎密切相关。然而,CC4 的特异性致病特征尚未确定。本研究对 144 株 C. sakazakii CC4 菌株和 376 株非 C. sakazakii CC4 菌株的基因组进行了比较分析。其中24株CC4菌株是以前未被描述过的临床和非临床菌株,它们采集自中国不同地区(2006-2017年)。其余的基因组是在全球范围内(1973-2021 年)分离到的多种序列类型的阪崎肠杆菌菌株。阪崎肠杆菌的泛基因组包括32332个基因,其中2.58%(832个基因)构成核心基因组。与非CC4菌株相比,更多的CC4菌株具有完整的cusABCFR外排系统,而且cusABCFR外排系统中的ibeB同源cusC基因可能与人脑微血管内皮细胞(BMEC)的入侵有关。此外,与新生儿脑膜炎相关的带有 K2:CA2 胶囊或 IV 型纤毛虫相关基因的分离株中,CC4 菌株比非 CC4 菌株在统计学上出现得更多(P < .0001)。此外,还详细描述了阪崎肠杆菌 IV 型分泌系统的 VirB/VirD4 基因簇以及 VI 型分泌系统的 impB/impF 和 vasL/vasJ 基因簇。总之,特异性和多样性的毒力因子和基因可能导致阪崎肠杆菌CC4克隆增强了侵入人类BMEC的能力,并导致新生儿脑膜炎。我们的研究结果将有助于开发预防、诊断和治疗C. sakazakii CC4感染的新策略。
{"title":"Genomic characterization of Cronobacter sakazakii strains in the neonatal meningitis clonal complex 4","authors":"Jinrui Hu, Zhenpeng Li, Yan Sun, Wenyu Shi, Xiaoli Du, Shuang Meng, Zhengfei Yu, Jinyue Liu, Stephen Forsythe, Zhigang Cui, Haijian Zhou","doi":"10.1002/fft2.401","DOIUrl":"10.1002/fft2.401","url":null,"abstract":"<p><i>Cronobacter sakazakii</i> clonal complex 4 (CC4) is strongly associated with neonatal meningitis. However, <i>C. sakazakii</i> CC4-specific pathogenicity traits have not been determined. In this study, the comparative genomic analysis of 144 genomes of <i>C. sakazakii</i> CC4 strains and 376 non-CC4 <i>C. sakazakii</i> strains was undertaken. Twenty-four CC4 strains were previously undescribed clinical and nonclinical strains that had been collected from various regions in China (2006–2017). The remaining genomes were of multiple sequence types of <i>C. sakazakii</i> strains, which had been isolated worldwide (1973–2021). The pan-genome of <i>C. sakazakii</i> comprised 32,332 genes, of which 2.58% (832 genes) constituted the core genome. More <i>C. sakazakii</i> CC4 strains have a complete <i>cusABCFR</i> efflux system with a significant difference compared with non-CC4 strains, and the <i>ibeB</i>-homologous <i>cusC</i> gene in the <i>cusABCFR</i> efflux system is probably associated with the invasion of human brain microvascular endothelial cells (BMEC). In addition, the isolates with K2:CA2 capsule or type IV pili-associated genes associated with neonatal meningitis were statistically more present in CC4 strains than non-CC4 strains (<i>p</i> < .0001). The thorough description of <i>VirB</i>/<i>VirD4</i> gene cluster for the type IV secretion system and the <i>impB</i>/<i>impF</i> and <i>vasL/vasJ</i> gene clusters for the type VI secretion system in <i>C. sakazakii</i> were provided. Overall, the specific and diverse virulence factors and genes may have led to <i>C. sakazakii</i> CC4 clone increasing its ability to invade human BMEC and leading to neonatal meningitis. Our findings should facilitate the development of novel strategies to prevent, diagnose, and treat <i>C. sakazakii</i> CC4 infections.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.401","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140667584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lactiplantibacillus plantarum is selective for carbohydrate utilization, which is primarily regulated by the catabolic control protein A (ccpA). To investigate the impact of carbohydrate metabolism on the in vivo colonization of L. plantarum AR113, we constructed a ccpA knockout strain (AR113ΔccpA). In vitro assays showed that AR113ΔccpA had a 0.34 decrease in maximum biomass, and a 2.63 h increase in hysteresis time compared to AR113. In a single administration, there was no significant difference in the number of AR113 and AR113ΔccpA in the mucus layers, and the number of AR113 was approximately 34-times higher than AR113ΔccpA at 48 h in the intestinal lumen. Notably, the knockout of the ccpA gene did not affect the colonization time of AR113 in the intestine during continuous administration. Therefore, the present work demonstrated that the ccpA did not play a crucial role in the in vivo colonization time of AR113 and provided valuable insights into the role of carbohydrate metabolism in bacterial colonization time in vivo.
{"title":"Effect of the catabolic control protein A of Lactiplantibacillus plantarum AR113 on its colonization in vivo","authors":"Wenfei Qin, Yue Zeng, Weilian Hung, Jiaqi Sun, Yongjun Xia, Zhiqiang Xiong, Xin Song, Lianzhong Ai, Guangqiang Wang","doi":"10.1002/fft2.378","DOIUrl":"10.1002/fft2.378","url":null,"abstract":"<p><i>Lactiplantibacillus plantarum</i> is selective for carbohydrate utilization, which is primarily regulated by the catabolic control protein A (ccpA). To investigate the impact of carbohydrate metabolism on the in vivo colonization of <i>L. plantarum</i> AR113, we constructed a ccpA knockout strain (AR113Δ<i>ccpA</i>). In vitro assays showed that AR113Δ<i>ccpA</i> had a 0.34 decrease in maximum biomass, and a 2.63 h increase in hysteresis time compared to AR113. In a single administration, there was no significant difference in the number of AR113 and AR113Δ<i>ccpA</i> in the mucus layers, and the number of AR113 was approximately 34-times higher than AR113Δ<i>ccpA</i> at 48 h in the intestinal lumen. Notably, the knockout of the ccpA gene did not affect the colonization time of AR113 in the intestine during continuous administration. Therefore, the present work demonstrated that the ccpA did not play a crucial role in the in vivo colonization time of AR113 and provided valuable insights into the role of carbohydrate metabolism in bacterial colonization time in vivo.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.378","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140795837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Caiyan Huang, Can Xiang, Fangzhou Wang, Christophe Blecker, Zhenyu Wang, Li Chen, Dequan Zhang
Phosphoglycerate kinase 1 (PGK1) and pyruvate kinase M2 (PKM2) have been identified as the postmortem meat quality biomarkers. However, the precise molecular mechanism through which they affect and regulate the development of meat quality remains unclear. In this work, the high- and low-activity groups (n = 10) were selected from 60 lamb muscles at 24 h postmortem based on the activity levels of PGK1 and PKM2. The metabolomic, proteomic, and transcriptomic analyses combined with deeply integrated multi-omics analysis were used to elucidate the mechanisms by which PGK1 and PKM2 characterize meat quality. The results indicated that glycolysis played a crucial role in regulating PGK1 and PKM2 activity at the metabolome, proteome, and transcriptome levels. In glycolysis pathway, we identified several key components closely related to PGK1 and PKM2 activity, including differential metabolites (adenosine triphosphate, adenosine diphosphate, glucose-6-phosphate, nicotinamide adenine dinucleotide phosphate, fructose-6-phosphate, dihydroxyacetone phosphate, 3-phosphoglycerate, NAD+ nicotinamide adenine dinucleotide, lactate, and pyruvate), different abundance proteins (lactate dehydrogenase B and fructose bisphosphate aldolase B), and differentially expressed genes (hexokinase and fructose-1,6-bisphosphatase 1). It was concluded that PGK1 and PKM2 may affect the formation of meat quality by regulating these critical substrates. Additionally, PGK1 and PKM2 could also affect the tricarboxylic acid cycle, oxidative phosphorylation, and muscle contraction in postmortem and then influence meat quality. This integrative omics study offers valuable insight into unraveling the molecular mechanisms underlying postmortem meat quality development.
{"title":"Integrated metabolome, proteome, and transcriptome analysis explored the molecular mechanism of phosphoglycerate kinase 1 and pyruvate kinase M2 characterizing the postmortem meat quality","authors":"Caiyan Huang, Can Xiang, Fangzhou Wang, Christophe Blecker, Zhenyu Wang, Li Chen, Dequan Zhang","doi":"10.1002/fft2.404","DOIUrl":"10.1002/fft2.404","url":null,"abstract":"<p>Phosphoglycerate kinase 1 (PGK1) and pyruvate kinase M2 (PKM2) have been identified as the postmortem meat quality biomarkers. However, the precise molecular mechanism through which they affect and regulate the development of meat quality remains unclear. In this work, the high- and low-activity groups (<i>n</i> = 10) were selected from 60 lamb muscles at 24 h postmortem based on the activity levels of PGK1 and PKM2. The metabolomic, proteomic, and transcriptomic analyses combined with deeply integrated multi-omics analysis were used to elucidate the mechanisms by which PGK1 and PKM2 characterize meat quality. The results indicated that glycolysis played a crucial role in regulating PGK1 and PKM2 activity at the metabolome, proteome, and transcriptome levels. In glycolysis pathway, we identified several key components closely related to PGK1 and PKM2 activity, including differential metabolites (adenosine triphosphate, adenosine diphosphate, glucose-6-phosphate, nicotinamide adenine dinucleotide phosphate, fructose-6-phosphate, dihydroxyacetone phosphate, 3-phosphoglycerate, NAD<sup>+</sup> nicotinamide adenine dinucleotide, lactate, and pyruvate), different abundance proteins (lactate dehydrogenase B and fructose bisphosphate aldolase B), and differentially expressed genes (hexokinase and fructose-1,6-bisphosphatase 1). It was concluded that PGK1 and PKM2 may affect the formation of meat quality by regulating these critical substrates. Additionally, PGK1 and PKM2 could also affect the tricarboxylic acid cycle, oxidative phosphorylation, and muscle contraction in postmortem and then influence meat quality. This integrative omics study offers valuable insight into unraveling the molecular mechanisms underlying postmortem meat quality development.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.404","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140691530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shuang Li, Songyi Lin, Pengfei Jiang, Zhijie Bao, Xixin Qian, Shuo Wang, Na Sun
Polysaccharides are widely used as quality improvers for meat products. However, the mechanisms of how different ionic polysaccharides regulate the gelling properties of myofibrillar protein (MP) are still unclear. This study aimed to elucidate the contribution of different ionic polysaccharides to MP gelation and its mechanism. The enhancement of hydrogen bond, hydrophobic interaction, and disulfide bond between carboxymethyl cellulose sodium (CMC-Na, anionic polysaccharide) and MP made them bind tightly, which contributes to the improvement of gel strength, water-holding capacity, and viscoelasticity. Konjac glucomannan (neutral polysaccharide) mainly relied on physical filling to support the gel network and improve the gel characteristics. The electrostatic attraction between cationic polysaccharides and MP enhanced the binding between them. However, due to the large structure of chitosan (cationic polysaccharide) sugar chain, it can only attach to the surface of protein, which limits the interaction between them. These findings will provide guidance for the application of polysaccharides as food quality improvers or fat substitutes and the design of new low-fat restructured meat products.
{"title":"The interaction mechanism of different ionic polysaccharides with myofibrillar protein and its contribution to the heat-induced gels","authors":"Shuang Li, Songyi Lin, Pengfei Jiang, Zhijie Bao, Xixin Qian, Shuo Wang, Na Sun","doi":"10.1002/fft2.403","DOIUrl":"10.1002/fft2.403","url":null,"abstract":"<p>Polysaccharides are widely used as quality improvers for meat products. However, the mechanisms of how different ionic polysaccharides regulate the gelling properties of myofibrillar protein (MP) are still unclear. This study aimed to elucidate the contribution of different ionic polysaccharides to MP gelation and its mechanism. The enhancement of hydrogen bond, hydrophobic interaction, and disulfide bond between carboxymethyl cellulose sodium (CMC-Na, anionic polysaccharide) and MP made them bind tightly, which contributes to the improvement of gel strength, water-holding capacity, and viscoelasticity. Konjac glucomannan (neutral polysaccharide) mainly relied on physical filling to support the gel network and improve the gel characteristics. The electrostatic attraction between cationic polysaccharides and MP enhanced the binding between them. However, due to the large structure of chitosan (cationic polysaccharide) sugar chain, it can only attach to the surface of protein, which limits the interaction between them. These findings will provide guidance for the application of polysaccharides as food quality improvers or fat substitutes and the design of new low-fat restructured meat products.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.403","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140691972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shipeng Yin, Liqiong Niu, Jian Zhang, Wei Yang, Yuanfa Liu
Beverages with nutritional functional properties constitute one of the fastest-growing segments in the food industry. Beverages produced with berries as raw materials, owing to their richness in various beneficial substances (such as fiber, vitamins, minerals, and bioactive phytochemicals), have emerged as integral components of nutritional functional beverages. In this review, we summarize the bioactive polyphenols present in berry beverages and discuss the potential properties of berries from an antidiabetic perspective. The application and impact of new technologies in the processing and production of berry beverages are also explored. Additionally, the review addresses the challenges and prospects faced by industrial development in berry beverage production. Our objective is to provide valuable information for food researchers and the berry beverage production industry, facilitating the production of highly nutritious drinks that appeal to consumers.
{"title":"Berry beverages: From bioactives to antidiabetes properties and beverage processing technology","authors":"Shipeng Yin, Liqiong Niu, Jian Zhang, Wei Yang, Yuanfa Liu","doi":"10.1002/fft2.399","DOIUrl":"10.1002/fft2.399","url":null,"abstract":"<p>Beverages with nutritional functional properties constitute one of the fastest-growing segments in the food industry. Beverages produced with berries as raw materials, owing to their richness in various beneficial substances (such as fiber, vitamins, minerals, and bioactive phytochemicals), have emerged as integral components of nutritional functional beverages. In this review, we summarize the bioactive polyphenols present in berry beverages and discuss the potential properties of berries from an antidiabetic perspective. The application and impact of new technologies in the processing and production of berry beverages are also explored. Additionally, the review addresses the challenges and prospects faced by industrial development in berry beverage production. Our objective is to provide valuable information for food researchers and the berry beverage production industry, facilitating the production of highly nutritious drinks that appeal to consumers.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.399","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140700405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ting Zhang, Zimeng Guo, Jiayin Che, Min Yan, Jingyimei Liang, Furong Wang, Jinhong Hu, Wei Song, Yahong Yuan, Tianli Yue
Patulin (PAT) is a common mycotoxin in moldy fruits that causes gastrointestinal injury if accidentally ingested. Some studies have reported the symptoms of intestinal injury caused by PAT. However, the effects of PAT on the gut microbiota and gut metabolism remain to be investigated, which is important for clarifying the toxicological mechanisms. Thus, C57/BL6J mice were gavaged once with 0, 1, 4, and 16 mg/kg body weight PAT, and the small intestinal and colonic contents were collected. The 16S rRNA sequencing and targeted and untargeted metabolomics were applied to assess the alterations in microbiota and metabolism, respectively. The results showed that Mycoplasma, Dubosiella, and Enterorhabdus were jointly affected by acute PAT exposure in the small intestinal and colonic contents. Some inflammation-related bacteria, such as Mycoplasma in the small intestine and Mycoplasma, Alistipes, Eubacterium_coprostanoligenes_group, and Candidatus_saccharimona in the colon, increased with increasing doses of PAT. In metabolic results, amino acids decreased and bile acids elevated in both intestinal contents; lipids increased in the small intestinal contents but decreased in the colonic contents. PAT mainly affected host amino acid and lipid metabolism; it also downregulated microbial tryptophan metabolism and short-chain fatty acid levels (mainly in the colon), which could be explained by the decrease of Bacteroides and Lactobacillus. These influences in microbial abundances and metabolic levels persisted after PAT and its products were cleared. These results fill a gap in the effects of PAT on the gut microbiota and provide a theoretical basis for elucidating the toxic mechanism of mycotoxins.
棒曲霉素(PAT)是霉变水果中常见的一种霉菌毒素,一旦误食会造成肠胃损伤。一些研究报告了 PAT 引起的肠道损伤症状。然而,PAT 对肠道微生物群和肠道新陈代谢的影响仍有待研究,这对阐明其毒性机制非常重要。因此,给 C57/BL6J 小鼠灌胃一次 0、1、4 和 16 mg/kg 体重的 PAT,并收集小肠和结肠内容物。应用 16S rRNA 测序以及靶向和非靶向代谢组学分别评估了微生物群和代谢的改变。结果显示,小肠和结肠内容物中的支原体、Dubosiella和Enterorhabdus共同受到急性PAT暴露的影响。一些与炎症有关的细菌,如小肠中的支原体和结肠中的支原体、Alistipes、Eubacterium_coprostanoligenes_group 和 Candidatus_saccharimona,随着 PAT 剂量的增加而增加。在代谢结果中,两种肠内容物中的氨基酸都减少了,胆汁酸增加了;小肠内容物中的脂质增加了,但结肠内容物中的脂质减少了。PAT 主要影响宿主的氨基酸和脂质代谢;它还降低了微生物的色氨酸代谢和短链脂肪酸水平(主要在结肠中),这可以用乳酸杆菌和乳酸杆菌的减少来解释。这些对微生物丰度和代谢水平的影响在 PAT 及其产物被清除后依然存在。这些结果填补了 PAT 对肠道微生物群影响方面的空白,为阐明霉菌毒素的毒性机制提供了理论依据。
{"title":"Dysbiosis of intestinal microbiota and metabolism caused by acute patulin exposure in mice","authors":"Ting Zhang, Zimeng Guo, Jiayin Che, Min Yan, Jingyimei Liang, Furong Wang, Jinhong Hu, Wei Song, Yahong Yuan, Tianli Yue","doi":"10.1002/fft2.397","DOIUrl":"10.1002/fft2.397","url":null,"abstract":"<p>Patulin (PAT) is a common mycotoxin in moldy fruits that causes gastrointestinal injury if accidentally ingested. Some studies have reported the symptoms of intestinal injury caused by PAT. However, the effects of PAT on the gut microbiota and gut metabolism remain to be investigated, which is important for clarifying the toxicological mechanisms. Thus, C57/BL6J mice were gavaged once with 0, 1, 4, and 16 mg/kg body weight PAT, and the small intestinal and colonic contents were collected. The 16S rRNA sequencing and targeted and untargeted metabolomics were applied to assess the alterations in microbiota and metabolism, respectively. The results showed that <i>Mycoplasma</i>, <i>Dubosiella</i>, and <i>Enterorhabdus</i> were jointly affected by acute PAT exposure in the small intestinal and colonic contents. Some inflammation-related bacteria, such as <i>Mycoplasma</i> in the small intestine and <i>Mycoplasma</i>, <i>Alistipes</i>, <i>Eubacterium_coprostanoligenes_group</i>, and <i>Candidatus_saccharimona</i> in the colon, increased with increasing doses of PAT. In metabolic results, amino acids decreased and bile acids elevated in both intestinal contents; lipids increased in the small intestinal contents but decreased in the colonic contents. PAT mainly affected host amino acid and lipid metabolism; it also downregulated microbial tryptophan metabolism and short-chain fatty acid levels (mainly in the colon), which could be explained by the decrease of <i>Bacteroides</i> and <i>Lactobacillus</i>. These influences in microbial abundances and metabolic levels persisted after PAT and its products were cleared. These results fill a gap in the effects of PAT on the gut microbiota and provide a theoretical basis for elucidating the toxic mechanism of mycotoxins.</p>","PeriodicalId":73042,"journal":{"name":"Food frontiers","volume":null,"pages":null},"PeriodicalIF":7.4,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fft2.397","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140715753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}