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Multi-omics approaches reveal inflammatory response and intestinal damage mediated by sRNA SaaS during Salmonella invasion in mice 多组学方法揭示沙门氏菌侵袭小鼠过程中由 sRNA SaaS 介导的炎症反应和肠道损伤
IF 7.4 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-10 DOI: 10.1002/fft2.421
Lin-lin Cai, Yun-ting Xie, Hai-jing Hu, Xing-lian Xu, Hu-hu Wang, Guang-hong Zhou

Salmonella Enteritidis is a foodborne enteric pathogen that infects humans and animals, and bacterial small RNAs (sRNAs) have been found to play critical roles in the virulence of Salmonella. However, the specific mechanism by which sRNAs contribute to the host–pathogen interaction in the intestine remains unclear. In this study, a combination of transcriptomic and proteomic analysis was used to investigate the effect of a particular sRNA known as Salmonella adhesive-associated sRNA (SaaS) on the intestinal damage induced by Salmonella. The results revealed that SaaS sRNA could suppress the host's inflammatory response through various gene sets, mainly including tumor necrosis factor (TNF)-α signaling via NF-κB, IL-6 JAK-STAT3 signaling, and interferon gamma responses. This suppressive effect was further confirmed by ELISA analysis, which showed the lowest levels of TNF-α, IL-6, and interferon gamma (IFN-γ). Meanwhile, the work has identified several crucial factors, peptidoglycan recognition protein short, transferrin receptor protein 1, and corticosteroid 11-beta-dehydrogenase isozyme 2, that are specifically targeted by SaaS to regulate inflammatory response, neutrophils activity, and diseases. Furthermore, combining hematologic analysis and correlation analysis, this study demonstrated that SaaS led to a decrease in the percentage of neutrophils and revealed a strong correlation between the three crucial factors and various markers of inflammation and intestinal barrier function. These findings provide a comprehensive understanding of how SaaS sRNA modulates Salmonella invasion in the animal intestine and further present a strategy on controlling foodborne Salmonella based on the new targets, sRNAs.

肠炎沙门氏菌(Salmonella Enteritidis)是一种食源性肠道病原体,可感染人类和动物,细菌小 RNA(sRNA)在沙门氏菌的毒力中发挥着关键作用。然而,sRNA 在肠道中促进宿主与病原体相互作用的具体机制仍不清楚。本研究结合转录组学和蛋白质组学分析,研究了一种被称为沙门氏菌粘附相关 sRNA(SaaS)的特殊 sRNA 对沙门氏菌诱导的肠道损伤的影响。结果发现,SaaS sRNA 可通过多种基因组抑制宿主的炎症反应,主要包括通过 NF-κB 的肿瘤坏死因子(TNF)-α 信号传导、IL-6 JAK-STAT3 信号传导和干扰素γ 反应。酶联免疫吸附分析进一步证实了这种抑制作用,该分析表明 TNF-α、IL-6 和γ干扰素(IFN-γ)的水平最低。同时,研究还发现了几个关键因子,即肽聚糖识别蛋白短、转铁蛋白受体蛋白1和皮质类固醇11-beta-脱氢酶同工酶2,它们是SaaS调节炎症反应、中性粒细胞活性和疾病的特异性靶标。此外,结合血液学分析和相关性分析,这项研究表明,SaaS 可导致中性粒细胞比例下降,并揭示了这三个关键因子与各种炎症指标和肠道屏障功能之间的强相关性。这些发现让人们全面了解了 SaaS sRNA 如何调节沙门氏菌在动物肠道中的侵袭,并进一步提出了一种基于新靶标 sRNA 的控制食源性沙门氏菌的策略。
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引用次数: 0
Kumquat pomace removal of free polyphenol alleviates induced acute enteritis and restores gut microbiota in dextran sodium sulphate-treated mice 去除游离多酚的金桔渣可缓解右旋糖酐硫酸钠处理小鼠诱发的急性肠炎并恢复肠道微生物区系
IF 7.4 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-07 DOI: 10.1002/fft2.400
Chunxiao Ji, Yixin Huang, Wenjie Xie, Yawen Zhu, Jingping Qin, Shiyin Guo, Hang Xiao, Wei Fan, Zhonghai Tang

Kumquat pomace non-extractable polyphenols (KNEP), which contains celobiotics and has anti-inflammatory and antioxidant effects, are one of the main components in kumquats. We aimed to determine the preventive and inhibitory effects of KNEP on dextran sodium sulphate (DSS)-induced inflammatory bowel disease (IBD). The disease activity index (DAI) score, H&E staining, high-throughput sequencing technology, quantitative analysis, ELISA, and western blotting were used to explore the effect of KNEP in an IBD mouse model. The DAI score and H&E staining results showed that compared with the DSS treatment group, the DAI and pathological status of IBD in the DSS + 3% KNEP (low-dose) and DSS + 6% KNEP (high-dose) intervention groups were significantly improved. High-throughput sequencing showed that the composition of the intestinal flora was restored under KNEP, and the abundance of pathogenic bacteria decreased and that of beneficial bacteria increased. Quantitative analysis of short-chain fatty acids (SCFAs) showed that the SCFAs content in the cecum of IBD mice increased in a dose-dependent manner. ELISA results showed that KNEP at different doses could inhibit the expression of TNF-α, IL-1β, IFN-γ, and other inflammatory factors, and that 6% KNEP had the most potent inhibitory effect. Western blotting results showed that KNEP inhibited the expression of TLR4, NF-κB, and NLRP3 in the colon of mice with DSS-induced colitis in a dose-dependent manner. These results indicate that KNEP can significantly improve DSS-induced acute colitis in mice, and the higher the KNEP content, the more pronounced the improvement in IBD.

金桔果渣不可提取多酚(KNEP)含有细胞生物素,具有抗炎和抗氧化作用,是金桔的主要成分之一。我们的目的是确定 KNEP 对葡聚糖硫酸钠(DSS)诱发的炎症性肠病(IBD)的预防和抑制作用。研究人员采用疾病活动指数(DAI)评分、H&E染色、高通量测序技术、定量分析、ELISA和Western印迹法来探讨KNEP在IBD小鼠模型中的作用。DAI评分和H&E染色结果显示,与DSS治疗组相比,DSS + 3% KNEP(低剂量)和DSS + 6% KNEP(高剂量)干预组的DAI和IBD病理状态均有明显改善。高通量测序显示,KNEP可恢复肠道菌群的组成,致病菌数量减少,有益菌数量增加。短链脂肪酸(SCFAs)定量分析显示,IBD小鼠盲肠中的SCFAs含量呈剂量依赖性增加。酶联免疫吸附试验结果表明,不同剂量的 KNEP 可抑制 TNF-α、IL-1β、IFN-γ 等炎症因子的表达,其中 6% 的 KNEP 的抑制作用最强。Western blotting结果显示,KNEP能以剂量依赖的方式抑制DSS诱导的小鼠结肠中TLR4、NF-κB和NLRP3的表达。这些结果表明,KNEP能明显改善DSS诱导的小鼠急性结肠炎,且KNEP含量越高,对IBD的改善越明显。
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引用次数: 0
Integrated analysis of transcriptomic and metabolomic data reveals how slurry ice treatment affects sugar metabolism in sweet corn (Zea mays L. var saccharata) during cold storage 转录组和代谢组数据的综合分析揭示了冰浆处理如何影响甜玉米(Zea mays L. var saccharata)冷藏期间的糖代谢
IF 7.4 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-07 DOI: 10.1002/fft2.410
Yaqi Zhao, Wenlin Shi, Zhiqiang Wang, Zhicheng Yan, Junyan Shi, Jinhua Zuo, Bihong Feng, Qing Wang

Sweet corn rapidly loses its sweetness, along with other nutrients, once it is harvested, which reduces its economic value. The present study was conducted to elucidate the regulatory mechanisms by which a rapid cooling slurry ice (SI) treatment followed by storage at 0 ± 0.5°C extends the postharvest quality of sweet corn relative to slower direct cooling at 0 ± 0.5°C. In this study, we found that SI treatment can maintain the appearance quality of sweet corn and inhibit grain shrinkage, respiration, and ethylene release. SI treatment delayed the aging of corn by regulating the expression levels of genes and the levels of metabolites. SI treatment also maintains its carbohydrate content, which is mainly related to starch and sucrose metabolism, glycolysis, citric acid cycle, pentose phosphate pathway, and cutin biosynthesis. The results provide a possible target for improving the postharvest quality of sweet corn.

甜玉米收获后会迅速失去甜味和其他营养成分,从而降低其经济价值。本研究旨在阐明快速冷却浆冰(SI)处理后在 0 ± 0.5°C 下贮藏相对于在 0 ± 0.5°C 下缓慢直接冷却可延长甜玉米收获后品质的调节机制。在这项研究中,我们发现 SI 处理可以保持甜玉米的外观质量,并抑制谷粒收缩、呼吸作用和乙烯释放。SI 处理通过调节基因的表达水平和代谢物的水平来延缓玉米的衰老。SI 处理还能保持其碳水化合物含量,这主要与淀粉和蔗糖代谢、糖酵解、柠檬酸循环、磷酸戊糖途径和角质素生物合成有关。这些结果为改善甜玉米收获后的品质提供了一个可能的目标。
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引用次数: 0
The hawthorn (Crataegus pinnatifida Bge.) fruit as a new dietary source of bioactive ingredients with multiple beneficial functions 山楂(Crataegus pinnatifida Bge.)果实作为具有多种有益功能的生物活性成分的新膳食来源
IF 7.4 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-07 DOI: 10.1002/fft2.413
Jin-Xin Ma, Wei Yang, Chester Yan Jie Ng, Xu-Dong Tang, Sunny Wong, Ren-You Gan, Linda Zhong

The discovery of new natural sources has brought increased attention to the development of functional foods. The hawthorn (Crataegus pinnatifida Bge.) fruit is an underutilized fruit due to its benefits for human health and good taste. It contains a variety of bioactive ingredients, contributing to its multiple beneficial functions and applications. This review summarized and discussed the main bioactive ingredients, beneficial functions based on in vitro, in vivo, and human studies, and different applications of the hawthorn fruit according to the updated literature in the past 3 years. Hawthorn berries contain phenolic acids, flavonoids, proanthocyanidins, pectin, and many other bioactive components, which have a variety of beneficial functions, such as antioxidant, anti-inflammatory, antibacterial, antidiabetic, intestinal protection, cardiovascular protection, hepatoprotection, anti-cancer, and neuroprotection. Its potential molecular mechanism and different food-related applications such as hawthorn wine and antioxidant drink are discussed in detail in this review. Additionally, hawthorn berries are shown to be safe when consumed within the proper dosage. Collectively, this updated review indicates that the hawthorn fruit can be a new dietary source of bioactive ingredients with multiple beneficial functions and can be affordably developed into functional and medicinal foods for the prevention and management of certain chronic diseases.

新天然来源的发现使人们越来越关注功能食品的开发。山楂(Crataegus pinnatifida Bge.)果实因其对人体健康的益处和良好的口感而成为一种未被充分利用的水果。它含有多种生物活性成分,具有多种有益功能和应用。本综述根据过去三年的最新文献,总结并讨论了山楂果的主要生物活性成分、基于体外、体内和人体研究的有益功能以及不同的应用。山楂果含有酚酸、黄酮类、原花青素、果胶等多种生物活性成分,具有抗氧化、抗炎、抗菌、抗糖尿病、肠道保护、心血管保护、保肝、抗癌、神经保护等多种有益功能。本综述详细讨论了其潜在的分子机制和不同的食品相关应用,如山楂酒和抗氧化饮料。此外,在适当剂量内食用山楂果是安全的。总之,这篇最新综述表明,山楂果可作为具有多种有益功能的生物活性成分的新膳食来源,并可开发成经济实惠的功能性和药用食品,用于预防和治疗某些慢性疾病。
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引用次数: 0
Effect of tea green leafhopper (Empoasca onukii Matsuda) sucking on the quality of Oriental Beauty 茶青叶蝉(Empoasca onukii Matsuda)吸食对 "东方美人 "品质的影响
IF 7.4 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-06 DOI: 10.1002/fft2.415
Lin Zeng, Yu-Yi Liu, Fang Wang, Shan Jin, Lian-Shuang Chen, Yan-Qing Fu, Zhi-Hui Feng, Jun-Feng Yin, Wei-Jiang Sun, Xiao-Min Yu, Yong-Quan Xu

This study aimed to investigate the effects of tea green leafhopper damage on the sensory quality and metabolite composition of Oriental Beauty by using tea leaves from different varieties of tea plants and processing factories. The results indicated that tea green leafhopper damage could reduce bitterness and increase the level of fruity aroma, which was related to the content changes of catechin components and alcohol substances. Furthermore, the tea green leafhopper salivary treatment experiment revealed that the decrease of catechin content and increase of theaflavin content were mainly influenced by the action of saliva and mechanical injury, and changes in volatile components such as linalool oxidation were responsible for the alteration in aroma. These findings provide a theoretical reference for the aroma formation and quality improvement of Oriental Beauty.

本研究以不同品种的茶树和不同加工厂的茶叶为研究对象,探讨茶青叶蝉危害对 "东方美人 "感官品质和代谢物组成的影响。结果表明,茶叶受到绿叶蝉危害后,苦涩味降低,果香味增加,这与儿茶素成分和醇类物质含量的变化有关。此外,茶青叶蝉唾液处理实验表明,儿茶素含量的降低和茶黄素含量的增加主要受唾液作用和机械损伤的影响,芳樟醇氧化等挥发性成分的变化是香气变化的原因。这些发现为 "东方美人 "的香气形成和品质改善提供了理论参考。
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引用次数: 0
Preliminary exploration of acceptance and emotional responses to the key floral volatile compounds of Pu'er crude tea 普洱粗茶主要花香挥发性化合物的接受和情感反应初探
IF 7.4 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-06 DOI: 10.1002/fft2.411
Huimin An, Xingchang Ou, Jinhua Chen, Juan Li, Shi Li, Yang Liu, Hongjian Jiang, Chongxing Li, Linjiang Fang, Zhonghua Liu, Jianan Huang

Pu'er crude tea (PCT), the initial raw material for making Pu'er tea, is favored because of unique flavor and health effects. Emotional response is helpful to explain consumers’ choice of different flavors of tea. This study aims to clarify the key floral volatiles of PCT and their emotional impact on people. Twenty-nine compounds were identified as the key volatiles from 237 volatiles, which formed the flowery aroma of PCT. Linalool, geraniol, nonanal, and methyl salicylate were more abundant in the PCT, they evoked the top five emotions (mild, wild, aggressive, tame, bored). Methyl salicylate contributed most to the wild emotion in the two mixed samples (B10 and B14), and it may contribute positively to the health effects of tea, such as refreshing and anti-inflammatory. This study provided a preliminary research idea for exploring the relationship between tea aroma and human emotions and its potential role in human health.

普洱粗茶(PCT)是制作普洱茶的初始原料,因其独特的风味和保健功效而备受青睐。情感反应有助于解释消费者对不同口味茶叶的选择。本研究旨在阐明普洱茶的主要花香挥发物及其对人的情感影响。从 237 种挥发物中鉴定出 29 种化合物为关键挥发物,它们构成了 PCT 的花香。芳樟醇、香叶醇、壬醛和水杨酸甲酯在 PCT 中含量较高,它们唤起了人们的五大情绪(温和、狂野、好斗、驯服、无聊)。在两个混合样本(B10 和 B14)中,水杨酸甲酯对野性情绪的影响最大,它可能对茶叶的健康功效(如提神和消炎)有积极作用。这项研究为探索茶香与人类情绪之间的关系及其对人类健康的潜在作用提供了一个初步的研究思路。
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引用次数: 0
Effect of cocoa consumption on postprandial blood pressure in older adults with untreated hypertension: A randomized crossover clinical trial 食用可可对未经治疗的老年高血压患者餐后血压的影响:随机交叉临床试验
IF 7.4 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-06 DOI: 10.1002/fft2.414
Emmanuel Ofori Osei, Isaac Amoah, Jia Jiet Lim, Phyllis Tawiah, Margaret Saka Aduama-Larbi, Ibok Nsa Oduro, Jesse Charles Cobbinah, Andrew Patrick Dare, Charles Diako

Cocoa powder is an important dietary source of flavanols that modulate elevated blood pressure (BP). This study aimed to investigate the acute effects of cocoa beverage co-consumption with a high-fat-high-salt meal (HFHSM) on postprandial systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate in older adults living with uncontrolled hypertension. The study was a randomized crossover trial and involved older adults (aged 52.50 ± 9.36 years) (n = 28) living with hypertension. Participants, following an overnight fast, consumed either an HFHSM and cocoa beverage comprising 15 g cocoa powder in 250 mL water (HFHSM + CB; intervention) or HFHSM and 250 mL water (HFHSM + W; control). Resting SBP, DBP, and the heart rate of participants were measured at baseline (−5 min and 0 min) and 15, 30, 45, 60, 90, and 120 min following the consumption of either HFHSM + CB or HFHSM + W using an automated BP monitor. Repeated measures linear mixed model was used to compare the effect of cocoa beverage and water on postprandial outcomes over a 2-h period. A significant reduction (−3.8 ± 0.6 mmHg, < .05) in resting SBP was observed postprandially following the intake of the HFHSM + CB over the 120-min period compared to the HFHSM + W. The effect was more prominent during the 90- and 120-min time points of the trial duration. No significant change in the DBP and heart rate following the consumption of HFHSM + CB compared to the HFHSM + W was observed. Cocoa beverage co-consumption with HFHSM improved postprandial SBP in older adults living with hypertension.

可可粉是黄烷醇的重要膳食来源,可调节血压(BP)升高。本研究旨在调查可可饮料与高脂高盐膳食(HFHSM)同时食用对未控制高血压的老年人餐后收缩压(SBP)、舒张压(DBP)和心率的急性影响。该研究是一项随机交叉试验,涉及患有高血压的老年人(年龄为 52.50 ± 9.36 岁)(n = 28)。参与者在一夜禁食后,饮用由 250 毫升水兑 15 克可可粉组成的 HFHSM 和可可饮料(HFHSM + CB;干预)或 HFHSM 和 250 毫升水(HFHSM + W;对照)。在基线(-5 分钟和 0 分钟)和食用 HFHSM + CB 或 HFHSM + W 后的 15、30、45、60、90 和 120 分钟,使用自动血压监测仪测量参与者的静态 SBP、DBP 和心率。采用重复测量线性混合模型比较了可可饮料和水在 2 小时内对餐后结果的影响。与 HFHSM + W 相比,120 分钟内摄入 HFHSM + CB 后餐后静息 SBP 明显降低(-3.8 ± 0.6 mmHg,p < .05)。与 HFHSM + W 相比,饮用 HFHSM + CB 后的 DBP 和心率没有明显变化。可可饮料与 HFHSM 同饮可改善患有高血压的老年人的餐后 SBP。
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引用次数: 0
Lonicera japonica Thunb. and its characteristic component chlorogenic acid alleviated experimental colitis by promoting Lactobacillus and fecal short-chain fatty acids production 忍冬及其特征成分绿原酸通过促进乳酸杆菌和粪便短链脂肪酸的产生缓解实验性结肠炎
IF 7.4 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-06 DOI: 10.1002/fft2.412
Jiebiao Chen, Wanhua Xu, Yang Liu, Xiao Liang, Yunyi Chen, Jiaojiao Liang, Jinping Cao, Baiyi Lu, Chongde Sun, Yue Wang

Ulcerative colitis is intricately linked to intestinal oxidative stress and dysbiosis of the gut microbiota. Lonicera japonica Thunb. (LJ) is a traditional edible and medical flower in China, and chlorogenic acid (CGA) is one of its characteristic components. However, it remains unclear whether gut microbiota plays a role in the therapeutic effects of LJ and GCA on colitis. Here, we first observed that oral administration of LJ and CGA for 3 weeks dramatically promoted the growth of Lactobacillus and fecal short-chain fatty acids (SCFAs) production in healthy mice. Subsequently, the alleviating effects of LJ and CGA on colitis were explored with a dextran sulfate sodium-induced colitis mice model. The intervention of LJ and CGA notably alleviated inflammation, intestinal barrier impairment, and oxidative stress in colitis and led to a significant elevation in Lactobacillus and fecal SCFAs. Eventually, the key role of gut microbiota and their metabolites on the therapeutic effects was validated by performing fecal microbiota transplantation and sterile fecal suspensions transplantation from LJ and CGA-treated healthy mice to colitis mice. Our findings demonstrated that consumption of LJ and CGA could benefit the host both in healthy condition and colitis. The beneficial effects were attributed to the improvement of the endogenous antioxidant system and promotion of the probiotic Lactobacillus and SCFAs production. Our study highlighted the great potential of LJ and CGA to be consumed as functional foods and provided novel mechanisms by which they alleviated colitis.

溃疡性结肠炎与肠道氧化应激和肠道微生物菌群失调密切相关。忍冬是中国传统的食用和药用花卉,绿原酸(CGA)是其特征成分之一。然而,肠道微生物群是否在忍冬花和绿原酸对结肠炎的治疗作用中发挥作用仍不清楚。在这里,我们首先观察到口服 LJ 和 CGA 3 周可显著促进健康小鼠体内乳酸杆菌的生长和粪便中短链脂肪酸(SCFAs)的产生。随后,研究人员利用葡聚糖硫酸钠诱导的结肠炎小鼠模型,探讨了 LJ 和 CGA 对结肠炎的缓解作用。LJ 和 CGA 的干预明显缓解了结肠炎的炎症、肠道屏障损伤和氧化应激,并导致乳酸杆菌和粪便 SCFAs 的显著增加。最后,通过将 LJ 和 CGA 处理过的健康小鼠的粪便微生物群移植和无菌粪悬液移植到结肠炎小鼠体内,验证了肠道微生物群及其代谢物对治疗效果的关键作用。我们的研究结果表明,食用 LJ 和 CGA 对健康状况和结肠炎的宿主都有益处。这些有益作用归因于改善了内源性抗氧化系统,促进了益生菌乳酸杆菌和 SCFAs 的产生。我们的研究强调了 LJ 和 CGA 作为功能食品的巨大潜力,并提供了它们缓解结肠炎的新机制。
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引用次数: 0
Taste and its receptors in human physiology: A comprehensive look 人体生理学中的味觉及其受体:全面了解
IF 7.4 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-02 DOI: 10.1002/fft2.407
Chao Li, Yaxin Li, Qing Sun, Aliya Abdurehim, Jiawen Xu, Junbo Xie, Yanqing Zhang

Increasing evidence shows that food has significance beyond traditional perception (providing nutrition and energy) in maintaining normal life activities. It is indicated that the sense of taste plays a crucial part in regulating human life activities. Taste is one of the basic physiological sensations in mammals, and it is the fundamental guarantee for them to perceive, select, and ingest nutrients in order to survive. With the advances in electrophysiology, molecular biology, and structural biology, studies on the intracellular and extracellular transduction mechanisms of taste have made great progress and gradually revealed the indispensable role of taste receptors in the regulation and maintenance of normal physiological activities. Up to now, how food regulates life activities through the taste pathway remains unclear. Thus, this review comprehensively and systematically summarizes the current study about the sense of taste, the function of taste receptors, the taste–structure relationship of gustatory molecules, the cross-talking between distinctive tastes, and the role of the gut–organ axis in the realization of taste. Moreover, we also provide forward-looking perspectives on taste research to afford a scientific basis for revealing the scientific connotation of taste receptors regulating body health.

越来越多的证据表明,食物在维持正常生命活动方面的意义超出了传统认知(提供营养和能量)。这表明,味觉在调节人类生命活动中起着至关重要的作用。味觉是哺乳动物的基本生理感觉之一,是它们感知、选择和摄取营养以维持生存的基本保障。随着电生理学、分子生物学和结构生物学的发展,味觉的胞内、胞外传导机制研究取得了重大进展,逐渐揭示了味觉受体在调节和维持正常生理活动中不可或缺的作用。迄今为止,食物如何通过味觉途径调节生命活动仍不清楚。因此,本综述全面系统地总结了目前关于味觉、味觉受体的功能、味觉分子的味觉结构关系、不同味觉之间的交叉作用以及肠道器官轴在味觉实现中的作用等方面的研究。此外,我们还提供了味觉研究的前瞻性视角,为揭示味觉受体调节人体健康的科学内涵提供了科学依据。
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引用次数: 0
Determining sugar and molasses origin by non-exchangeable hydrogen stable isotope of ethanol and carbon isotope ratio mass spectrometry 用乙醇的非交换氢稳定同位素和碳同位素比质谱法确定糖和糖蜜的来源
IF 7.4 Q1 FOOD SCIENCE & TECHNOLOGY Pub Date : 2024-05-02 DOI: 10.1002/fft2.418
Macarena Rojas-Rioseco, Hafiz Imran Fakhar, Ivan Smajlovic, Margarita Smajlovic, Stevan Grkavac, Mecit Halil Oztop, Muhammad Qasim Hayat, Rosario del P. Castillo

This study explores the differentiation of sugar and molasses produced from sugar beet and cane, which are susceptible to fraudulent labeling due to differing production costs. The research aimed to authenticate these products by botanical origin using novel analytical techniques. Utilizing ethanol isotopic measurement–isotope ratio mass spectrometry (IRMS) for non-exchangeable hydrogen stable isotopes alongside carbon stable isotopes analysis through elemental analyzer–IRMS, the study accurately identified the origin of various sugar and molasses samples, pinpointed mislabeled goods, and determined the source of products with previously unknown provenance. These methods were also effective in revealing sugar and molasses adulteration and quantifying the extent of such fraud. The combined isotope analyses demonstrated their potential as robust tools for combating misrepresentation and adulteration in the sugar industry.

由于甜菜和甘蔗的生产成本不同,这两种糖很容易被贴上欺诈性标签,本研究探讨了如何区分这两种糖。研究旨在利用新型分析技术,通过植物来源鉴定这些产品的真伪。该研究利用乙醇同位素测量-同位素比质谱法(IRMS)对非交换性氢稳定同位素进行测量,同时利用元素分析仪-IRMS 对碳稳定同位素进行分析,准确鉴定了各种糖和糖蜜样品的来源,找出了贴错标签的商品,并确定了以前来源不明的产品的来源。这些方法还能有效揭示糖和糖浆的掺假情况,并量化此类欺诈行为的程度。综合同位素分析证明了它们作为打击制糖业虚假宣传和掺假行为的有力工具的潜力。
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引用次数: 0
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