Pub Date : 2023-06-01DOI: 10.1089/genbio.2023.29100.sra
Sachin Rawat
{"title":"The Need for Speed and Energy Efficiency in Genome Analysis","authors":"Sachin Rawat","doi":"10.1089/genbio.2023.29100.sra","DOIUrl":"https://doi.org/10.1089/genbio.2023.29100.sra","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47577003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1089/genbio.2023.0012
T. Linder
{"title":"Fulfilling the Promises of Fermentation-Derived Foods","authors":"T. Linder","doi":"10.1089/genbio.2023.0012","DOIUrl":"https://doi.org/10.1089/genbio.2023.0012","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49444072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1089/genbio.2023.0016
D. Mugahid, Till Hoffmann, J. Olejarz, R. Molinaro, Charles Cooper, R. Atun, Y. Grad, Sarah Fortune, R. Sampath, J. Onnela
{"title":"Digitally Connected Diagnostics Data in the Future of Public Health","authors":"D. Mugahid, Till Hoffmann, J. Olejarz, R. Molinaro, Charles Cooper, R. Atun, Y. Grad, Sarah Fortune, R. Sampath, J. Onnela","doi":"10.1089/genbio.2023.0016","DOIUrl":"https://doi.org/10.1089/genbio.2023.0016","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45967607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01Epub Date: 2023-06-19DOI: 10.1089/genbio.2023.0024
Simrita Deol, Patrick S Donahue, Roxana E Mitrut, Iva J Hammitt-Kess, Jihae Ahn, Bin Zhang, Joshua N Leonard
Off-the shelf immune cell therapies are potentially curative and may offer cost and manufacturing advantages over autologous products, but further development is needed. The NK92 cell line has a natural killer-like phenotype, has efficacy in cancer clinical trials, and is safe after irradiation. However, NK92 cells lose activity post-injection, limiting efficacy. This may be addressed by engineering NK92 cells to express stimulatory factors, and comparative analysis is needed. Thus, we systematically explored the expression of synthetic cytokines for enhancing NK92 cell production and performance. All synthetic cytokines evaluated (membrane-bound IL2 and IL15, and engineered versions of Neoleukin-2/15, IL15, IL12, and decoy resistant IL18) enhanced NK92 cell cytotoxicity. Engineered cells were preferentially expanded by expressing membrane-bound but not soluble synthetic cytokines, without compromising the radiosensitivity required for safety. Some membrane-bound cytokines conferred cell-contact independent paracrine activity, partly attributable to extracellular vesicles. Finally, we characterized interactions within consortia of differently engineered NK92 cells.
{"title":"Comparative Evaluation of Synthetic Cytokines for Enhancing Production and Performance of NK92 Cell-Based Therapies.","authors":"Simrita Deol, Patrick S Donahue, Roxana E Mitrut, Iva J Hammitt-Kess, Jihae Ahn, Bin Zhang, Joshua N Leonard","doi":"10.1089/genbio.2023.0024","DOIUrl":"10.1089/genbio.2023.0024","url":null,"abstract":"<p><p>Off-the shelf immune cell therapies are potentially curative and may offer cost and manufacturing advantages over autologous products, but further development is needed. The NK92 cell line has a natural killer-like phenotype, has efficacy in cancer clinical trials, and is safe after irradiation. However, NK92 cells lose activity post-injection, limiting efficacy. This may be addressed by engineering NK92 cells to express stimulatory factors, and comparative analysis is needed. Thus, we systematically explored the expression of synthetic cytokines for enhancing NK92 cell production and performance. All synthetic cytokines evaluated (membrane-bound IL2 and IL15, and engineered versions of Neoleukin-2/15, IL15, IL12, and decoy resistant IL18) enhanced NK92 cell cytotoxicity. Engineered cells were preferentially expanded by expressing membrane-bound but not soluble synthetic cytokines, without compromising the radiosensitivity required for safety. Some membrane-bound cytokines conferred cell-contact independent paracrine activity, partly attributable to extracellular vesicles. Finally, we characterized interactions within consortia of differently engineered NK92 cells.</p>","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"2 3","pages":"228-246"},"PeriodicalIF":2.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10286265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9718288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01Epub Date: 2023-06-19DOI: 10.1089/genbio.2023.0017
Yajuan Li, Phyllis Chang, Shriya Sankaran, Hongje Jang, Yuhang Nie, Audrey Zeng, Sahran Hussain, Jane Y Wu, Xu Chen, Lingyan Shi
Studies have shown that brain lipid metabolism is associated with biological aging and influenced by dietary and genetic manipulations; however, the underlying mechanisms are elusive. High-resolution imaging techniques propose a novel and potent approach to understanding lipid metabolic dynamics in situ. Applying deuterium water (D2O) probing with stimulated Raman scattering (DO-SRS) microscopy, we revealed that lipid metabolic activity in Drosophila brain decreased with aging in a sex-dependent manner. Female flies showed an earlier occurrence of lipid turnover decrease than males. Dietary restriction (DR) and downregulation of insulin/IGF-1 signaling (IIS) pathway, two scenarios for lifespan extension, led to significant enhancements of brain lipid turnover in old flies. Combining SRS imaging with deuterated bioorthogonal probes (deuterated glucose and deuterated acetate), we discovered that, under DR treatment and downregulation of IIS pathway, brain metabolism shifted to use acetate as a major carbon source for lipid synthesis. For the first time, our study directly visualizes and quantifies spatiotemporal alterations of lipid turnover in Drosophila brain at the single organelle (lipid droplet) level. Our study not only demonstrates a new approach for studying brain lipid metabolic activity in situ but also illuminates the interconnection of aging, dietary, and genetic manipulations on brain lipid metabolic regulation.
{"title":"Bioorthogonal Stimulated Raman Scattering Imaging Uncovers Lipid Metabolic Dynamics in <i>Drosophila</i> Brain During Aging.","authors":"Yajuan Li, Phyllis Chang, Shriya Sankaran, Hongje Jang, Yuhang Nie, Audrey Zeng, Sahran Hussain, Jane Y Wu, Xu Chen, Lingyan Shi","doi":"10.1089/genbio.2023.0017","DOIUrl":"10.1089/genbio.2023.0017","url":null,"abstract":"<p><p>Studies have shown that brain lipid metabolism is associated with biological aging and influenced by dietary and genetic manipulations; however, the underlying mechanisms are elusive. High-resolution imaging techniques propose a novel and potent approach to understanding lipid metabolic dynamics <i>in situ</i>. Applying deuterium water (D<sub>2</sub>O) probing with stimulated Raman scattering (DO-SRS) microscopy, we revealed that lipid metabolic activity in <i>Drosophila</i> brain decreased with aging in a sex-dependent manner. Female flies showed an earlier occurrence of lipid turnover decrease than males. Dietary restriction (DR) and downregulation of insulin/IGF-1 signaling (IIS) pathway, two scenarios for lifespan extension, led to significant enhancements of brain lipid turnover in old flies. Combining SRS imaging with deuterated bioorthogonal probes (deuterated glucose and deuterated acetate), we discovered that, under DR treatment and downregulation of IIS pathway, brain metabolism shifted to use acetate as a major carbon source for lipid synthesis. For the first time, our study directly visualizes and quantifies spatiotemporal alterations of lipid turnover in <i>Drosophila</i> brain at the single organelle (lipid droplet) level. Our study not only demonstrates a new approach for studying brain lipid metabolic activity <i>in situ</i> but also illuminates the interconnection of aging, dietary, and genetic manipulations on brain lipid metabolic regulation.</p>","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":"2 3","pages":"247-261"},"PeriodicalIF":2.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10286263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9718289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1089/genbio.2023.29103.jdg
Jonathan D. Grinstein, A. Philippidis
{"title":"Beyond Busulfan: Building a Better Conditioner for Bone Marrow Transplantation","authors":"Jonathan D. Grinstein, A. Philippidis","doi":"10.1089/genbio.2023.29103.jdg","DOIUrl":"https://doi.org/10.1089/genbio.2023.29103.jdg","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43036852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1089/genbio.2023.29096.mar
Mandana Arbab
{"title":"Predicting and Improving Insertions by Prime Editing","authors":"Mandana Arbab","doi":"10.1089/genbio.2023.29096.mar","DOIUrl":"https://doi.org/10.1089/genbio.2023.29096.mar","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48876741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.1089/genbio.2023.0015
Sunmee Choi, M. You, Yun-A Jeon, Jaebok Lee, Jinhwa Kim, Young Jin Park, J. Kim, Jong-Wook Park, Jae Kwang Kim, Sunghwa Choe
{"title":"Metabolic Engineering to Enhance Provitamin D3 Accumulation in Edible Tomatoes","authors":"Sunmee Choi, M. You, Yun-A Jeon, Jaebok Lee, Jinhwa Kim, Young Jin Park, J. Kim, Jong-Wook Park, Jae Kwang Kim, Sunghwa Choe","doi":"10.1089/genbio.2023.0015","DOIUrl":"https://doi.org/10.1089/genbio.2023.0015","url":null,"abstract":"","PeriodicalId":73134,"journal":{"name":"GEN biotechnology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46775455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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