Abstract Little is known about sexual behavior and satisfaction among men who have sex with men (MSM) with detectable HIV viral loads (VL) in China. This study aims to investigate sexual risk behavior and sexual satisfaction among MSM living with HIV in China, as well as potential predictors. This cross-sectional study was conducted in February 2021 using an anonymous online survey based on WeChat. Data were collected on sociodemographics, sexual behavior, HIV clinical information, sexual satisfaction and mental health. Descriptive statistics, logistic regression and multivariable logistic regression were performed. MSM with detectable HIV VL (VL+) or VL not sure were more likely to report inconsistent condom use, even with HIV-negative/unknown-status regular male partners. Having an HIV-positive regular partner and recreational drug use were positively associated with inconsistent condom use during anal sex among MSM with undetectable VL (VL−). Alcohol use before or during sexual activities was positively associated with inconsistent condom use during anal sex among VL+ or VL not sure MSM. A total of 58.3% of participants reported being sexually satisfied. MSM living with HIV who were currently employed, had experienced anal sex in the prior 6 months, had VL− HIV status, or had more frequent ejaculation during sex/masturbation were more likely to report sexual satisfaction. The development of targeted education and interventions to minimize sexual risk behavior among MSM living with HIV is in dire need. Treating sexual dysfunction and developing a reasonable antiretroviral therapy regimen to achieve viral suppression can help promote sexual satisfaction in MSM living with HIV.
{"title":"Sexual Risk Behavior and Satisfaction Among Men Who Have Sex With Men Living With Detectable HIV Viral Loads: A Nationwide Online Survey in China","authors":"Leiwen Fu, Bingyi Wang, Tian Tian, Xinyi Zhou, Zhen Lu, Yinghui Sun, Weiran Zheng, Yanxiao Gao, Yi-Fan Lin, Hui Li, H. Zou","doi":"10.1097/IM9.0000000000000124","DOIUrl":"https://doi.org/10.1097/IM9.0000000000000124","url":null,"abstract":"Abstract Little is known about sexual behavior and satisfaction among men who have sex with men (MSM) with detectable HIV viral loads (VL) in China. This study aims to investigate sexual risk behavior and sexual satisfaction among MSM living with HIV in China, as well as potential predictors. This cross-sectional study was conducted in February 2021 using an anonymous online survey based on WeChat. Data were collected on sociodemographics, sexual behavior, HIV clinical information, sexual satisfaction and mental health. Descriptive statistics, logistic regression and multivariable logistic regression were performed. MSM with detectable HIV VL (VL+) or VL not sure were more likely to report inconsistent condom use, even with HIV-negative/unknown-status regular male partners. Having an HIV-positive regular partner and recreational drug use were positively associated with inconsistent condom use during anal sex among MSM with undetectable VL (VL−). Alcohol use before or during sexual activities was positively associated with inconsistent condom use during anal sex among VL+ or VL not sure MSM. A total of 58.3% of participants reported being sexually satisfied. MSM living with HIV who were currently employed, had experienced anal sex in the prior 6 months, had VL− HIV status, or had more frequent ejaculation during sex/masturbation were more likely to report sexual satisfaction. The development of targeted education and interventions to minimize sexual risk behavior among MSM living with HIV is in dire need. Treating sexual dysfunction and developing a reasonable antiretroviral therapy regimen to achieve viral suppression can help promote sexual satisfaction in MSM living with HIV.","PeriodicalId":73374,"journal":{"name":"Infectious microbes & diseases","volume":"5 1","pages":"137 - 144"},"PeriodicalIF":0.0,"publicationDate":"2023-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45109230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-08DOI: 10.1097/IM9.0000000000000123
Emily J. Baker, Antonia Molloy, Jonathan A. G. Cox
Abstract With the ever-increasing burden of antimicrobial resistance, the demand to introduce countermeasures becomes increasingly critical. The urgency of this need is intensified by the void in antibiotic discovery, with the identification of novel compounds declining with time. Of increasing concern is Mycobacterium abscessus, which displays high levels of intrinsic resistance that lead to poor success rates, even after lengthy drug regimens. Research tackling these issues is now focused on the repurposing of preexisting drugs for antimycobacterial use, facilitating the discovery of antimicrobial compounds amidst a crisis where novel antibiotics are sparse. Part of this includes the use of combination treatments, whereby coadministration of synergistic compounds can reduce dose requirements and slow the progression of antimicrobial resistance in the long term. In this review, we will introduce the current therapeutic options for M. abscessus and provide insight into why treatment is so burdensome. We will also compile the current updates within drug repurposing for this pathogen, including the use of unconventional agents such as antimalarial drugs, the repositioning of antituberculosis candidates and the repurposing of preexisting antibiotics, including the application of combination regimens. In addition, the in vitro drug screening platforms used in their discovery will be appraised, with the view of highlighting potential future perspectives that may help increase physiological relevance. This review provides a timely appraisal of the future of M. abscessus treatment.
{"title":"Drug Repurposing for the Treatment of Mycobacterium abscessus Infections","authors":"Emily J. Baker, Antonia Molloy, Jonathan A. G. Cox","doi":"10.1097/IM9.0000000000000123","DOIUrl":"https://doi.org/10.1097/IM9.0000000000000123","url":null,"abstract":"Abstract With the ever-increasing burden of antimicrobial resistance, the demand to introduce countermeasures becomes increasingly critical. The urgency of this need is intensified by the void in antibiotic discovery, with the identification of novel compounds declining with time. Of increasing concern is Mycobacterium abscessus, which displays high levels of intrinsic resistance that lead to poor success rates, even after lengthy drug regimens. Research tackling these issues is now focused on the repurposing of preexisting drugs for antimycobacterial use, facilitating the discovery of antimicrobial compounds amidst a crisis where novel antibiotics are sparse. Part of this includes the use of combination treatments, whereby coadministration of synergistic compounds can reduce dose requirements and slow the progression of antimicrobial resistance in the long term. In this review, we will introduce the current therapeutic options for M. abscessus and provide insight into why treatment is so burdensome. We will also compile the current updates within drug repurposing for this pathogen, including the use of unconventional agents such as antimalarial drugs, the repositioning of antituberculosis candidates and the repurposing of preexisting antibiotics, including the application of combination regimens. In addition, the in vitro drug screening platforms used in their discovery will be appraised, with the view of highlighting potential future perspectives that may help increase physiological relevance. This review provides a timely appraisal of the future of M. abscessus treatment.","PeriodicalId":73374,"journal":{"name":"Infectious microbes & diseases","volume":"5 1","pages":"101 - 110"},"PeriodicalIF":0.0,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44100343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Currently, tuberculosis (TB) is the second most lethal disease in the world caused by a single infectious pathogen. Rapid diagnosis of TB is of great importance for its treatment and management. Xpert MTB/RIF is a novel rapid diagnostic assay for the diagnosis of pulmonary TB (PTB). Use of the Xpert assay based on bronchoalveolar lavage fluid (BALF) samples is indicated when TB is suspected and sputum smears or cultures are negative. The aim of this meta-analysis was to systematically evaluate the diagnostic performance of the Xpert assay based on BALF samples for the diagnosis of PTB. A systematic review of previously published articles was performed, and relevant data were extracted. Meta-DiSc 1.4 and Stata 12.0 were used to analyze the data. When Mycobacterium tuberculosis cultures were used as the criterion standard, the combined sensitivity of BALF-based Xpert was 0.89 (95% CI, 0.87–0.91), the specificity was 0.87 (95% CI, 0.85-0.88), the positive likelihood ratio was 8.28 (95% CI, 5.39–12.71), the negative likelihood ratio was 0.14 (95% CI, 0.10–0.19) and the diagnostic ratio was 84.08 (95% CI, 42.00–168.31). When composite reference standard was used as the criterion standard, the above observations were 0.69 (95% CI, 0.67–0.72), 0.98 (95% CI, 0.97–0.98), 41.40 (95% CI, 14.56–117.71), 0.28 (95% CI, 0.21–0.37) and 190.47 (95% CI, 50.56–717.54), respectively. The area under the summary receiver operating characteristic curve was close to 1 for both. Overall, the Xpert MTB/RIF assay based on BALF samples showed high sensitivity and specificity for the diagnosis of PTB and seems to be a reliable rapid detection method.
{"title":"Xpert MTB/RIF Assay for the Diagnosis of Pulmonary Tuberculosis Based on Bronchoalveolar Lavage Fluid: A Systematic Review and Meta-analysis","authors":"Hao Chen, Chun-Xi Lin, Peng-Li Lv, Wen-feng Li, Yu-Ying Feng, X. Deng, Jianhan Xiao, Xu-guang Guo","doi":"10.1097/IM9.0000000000000122","DOIUrl":"https://doi.org/10.1097/IM9.0000000000000122","url":null,"abstract":"Abstract Currently, tuberculosis (TB) is the second most lethal disease in the world caused by a single infectious pathogen. Rapid diagnosis of TB is of great importance for its treatment and management. Xpert MTB/RIF is a novel rapid diagnostic assay for the diagnosis of pulmonary TB (PTB). Use of the Xpert assay based on bronchoalveolar lavage fluid (BALF) samples is indicated when TB is suspected and sputum smears or cultures are negative. The aim of this meta-analysis was to systematically evaluate the diagnostic performance of the Xpert assay based on BALF samples for the diagnosis of PTB. A systematic review of previously published articles was performed, and relevant data were extracted. Meta-DiSc 1.4 and Stata 12.0 were used to analyze the data. When Mycobacterium tuberculosis cultures were used as the criterion standard, the combined sensitivity of BALF-based Xpert was 0.89 (95% CI, 0.87–0.91), the specificity was 0.87 (95% CI, 0.85-0.88), the positive likelihood ratio was 8.28 (95% CI, 5.39–12.71), the negative likelihood ratio was 0.14 (95% CI, 0.10–0.19) and the diagnostic ratio was 84.08 (95% CI, 42.00–168.31). When composite reference standard was used as the criterion standard, the above observations were 0.69 (95% CI, 0.67–0.72), 0.98 (95% CI, 0.97–0.98), 41.40 (95% CI, 14.56–117.71), 0.28 (95% CI, 0.21–0.37) and 190.47 (95% CI, 50.56–717.54), respectively. The area under the summary receiver operating characteristic curve was close to 1 for both. Overall, the Xpert MTB/RIF assay based on BALF samples showed high sensitivity and specificity for the diagnosis of PTB and seems to be a reliable rapid detection method.","PeriodicalId":73374,"journal":{"name":"Infectious microbes & diseases","volume":"5 1","pages":"111 - 119"},"PeriodicalIF":0.0,"publicationDate":"2023-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44803325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-03DOI: 10.1097/IM9.0000000000000118
A. Murugananthan, Kanchana P. Amarasinghe, G. Rathnaweera, Ponnathurai T. Amalraajan, Rajadurai Arulenthiran
Abstract Leishmania parasites undergo a dimorphic life cycle. Diagnosis of cutaneous leishmaniasis (CL) is primarily made by the microscopic identification of the amastigote form of the parasite in a smear. Therefore, correct identification of Leishman-Donovan (LD) bodies in smears is crucial for CL confirmation. The aim of this study was to provide a broad overview of different cytomorphologies of LD bodies to improve the sensitivity of microscopic detection of Leishmania donovani, which causes CL in Sri Lanka. A total of 125 smears, prepared from patients who met the positive diagnostic criteria of CL, were Giemsa stained and examined microscopically. The density, cellular location and all possible cytomorphological forms of the parasite were documented. A total of 10 different cytomorphological forms of LD bodies were identified. Among them, the round form was observed only intracellularly. The other morphological forms, namely the pear shape, cigarette shape, candle flame form, embryo shape, flagellate form, binary form and rosette form, were found only extracellularly. The spindle form and lens shape were observed in both intracellular and extracellular locations. L. donovani amastigotes have a wide range of morphologies besides their classical forms. Although Leishmania are considered as obligatory intracellular parasites, they manage to survive successfully within the extracellular hostile conditions as well. Therefore, having a broader view of different morphological forms for Leishmania parasites may help to improve diagnosis of CL.
{"title":"Cytomorphological Variations of Leishman-Donovan Bodies Found in Cutaneous Leishmaniasis Patients in Sri Lanka","authors":"A. Murugananthan, Kanchana P. Amarasinghe, G. Rathnaweera, Ponnathurai T. Amalraajan, Rajadurai Arulenthiran","doi":"10.1097/IM9.0000000000000118","DOIUrl":"https://doi.org/10.1097/IM9.0000000000000118","url":null,"abstract":"Abstract Leishmania parasites undergo a dimorphic life cycle. Diagnosis of cutaneous leishmaniasis (CL) is primarily made by the microscopic identification of the amastigote form of the parasite in a smear. Therefore, correct identification of Leishman-Donovan (LD) bodies in smears is crucial for CL confirmation. The aim of this study was to provide a broad overview of different cytomorphologies of LD bodies to improve the sensitivity of microscopic detection of Leishmania donovani, which causes CL in Sri Lanka. A total of 125 smears, prepared from patients who met the positive diagnostic criteria of CL, were Giemsa stained and examined microscopically. The density, cellular location and all possible cytomorphological forms of the parasite were documented. A total of 10 different cytomorphological forms of LD bodies were identified. Among them, the round form was observed only intracellularly. The other morphological forms, namely the pear shape, cigarette shape, candle flame form, embryo shape, flagellate form, binary form and rosette form, were found only extracellularly. The spindle form and lens shape were observed in both intracellular and extracellular locations. L. donovani amastigotes have a wide range of morphologies besides their classical forms. Although Leishmania are considered as obligatory intracellular parasites, they manage to survive successfully within the extracellular hostile conditions as well. Therefore, having a broader view of different morphological forms for Leishmania parasites may help to improve diagnosis of CL.","PeriodicalId":73374,"journal":{"name":"Infectious microbes & diseases","volume":"5 1","pages":"93 - 100"},"PeriodicalIF":0.0,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43896251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-29DOI: 10.1097/IM9.0000000000000117
Shu-Chang Weng, Jie Zhou, Xiaoni Zhong, Qing Xin, Yue-Xue Mai, Li Ji, Ya-ping Li, Xun-Jie Cao, W. Yang, Hao Chen, Xu-guang Guo
Abstract Hepatitis C virus (HCV) is a globally widespread ribonucleic acid virus that transmits through blood and sexual contact. Its morbidity and mortality are particularly higher in economically underdeveloped areas. Therefore, an economical and effective diagnostic method for detection of HCV is urgently needed. In this study, we evaluated the diagnostic accuracy of the SD BIOLINE rapid diagnostic test for HCV detection. We searched for studies related to SD BIOLINE and HCV in PubMed, Embase, Web of Science, and the Cochrane Library and then designed inclusion and exclusion criteria. After extracting valid data, the included literature was evaluated with the quality assessment tool Quality Assessment of Diagnostic Accuracy Studies. After our data analysis, the sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic accuracy, summary receiver operating characteristic curve, funnel plot, box plot, and Fagan plot of the diagnostic method were determined. Nine articles with nine sets of data were finally included. The sensitivity and specificity were 0.94 and 0.98, respectively, the positive likelihood ratio was 79.53, the negative likelihood ratio was 0.05, the diagnostic odds ratio was 1590.32, and the summary receiver operating characteristic curve was 0.9958. The SD BIOLINE test has the advantages of high sensitivity, high specificity, low cost, and easy operation for diagnosing HCV. Therefore, we recommend using SD BIOLINE for rapid and effective screening of HCV, which is especially applicable for economically underdeveloped areas.
摘要丙型肝炎病毒（HCV）是一种在全球广泛传播的核糖核酸病毒，通过血液和性接触传播。在经济欠发达地区，其发病率和死亡率尤其高。因此，迫切需要一种经济有效的丙型肝炎病毒诊断方法。在本研究中，我们评估了SD BIOLINE快速诊断试验对丙型肝炎病毒检测的诊断准确性。我们在PubMed、Embase、Web of Science和Cochrane Library中搜索了与SD BIOLINE和HCV相关的研究，然后设计了纳入和排除标准。在提取有效数据后，使用质量评估工具诊断准确性研究的质量评估对纳入的文献进行评估。在我们的数据分析后，确定了诊断方法的灵敏度、特异性、阳性似然比、阴性似然比、诊断准确度、总结受试者操作特征曲线、漏斗图、箱图和Fagan图。最终收录了九篇文章和九组数据。敏感性和特异性分别为0.94和0.98，阳性似然比为79.53，阴性似然比为0.05，诊断优势比为1590.32，总结受试者工作特性曲线为0.9958。SD BIOLINE试验诊断丙型肝炎具有灵敏度高、特异性强、成本低、操作简便等优点。因此，我们建议使用SD BIOLINE快速有效地筛查丙型肝炎病毒，特别适用于经济欠发达地区。
{"title":"Assessing the Diagnostic Accuracy of SD BIOLINE for Detection of Hepatitis C Virus: A Systematic Review and Meta-analysis","authors":"Shu-Chang Weng, Jie Zhou, Xiaoni Zhong, Qing Xin, Yue-Xue Mai, Li Ji, Ya-ping Li, Xun-Jie Cao, W. Yang, Hao Chen, Xu-guang Guo","doi":"10.1097/IM9.0000000000000117","DOIUrl":"https://doi.org/10.1097/IM9.0000000000000117","url":null,"abstract":"Abstract Hepatitis C virus (HCV) is a globally widespread ribonucleic acid virus that transmits through blood and sexual contact. Its morbidity and mortality are particularly higher in economically underdeveloped areas. Therefore, an economical and effective diagnostic method for detection of HCV is urgently needed. In this study, we evaluated the diagnostic accuracy of the SD BIOLINE rapid diagnostic test for HCV detection. We searched for studies related to SD BIOLINE and HCV in PubMed, Embase, Web of Science, and the Cochrane Library and then designed inclusion and exclusion criteria. After extracting valid data, the included literature was evaluated with the quality assessment tool Quality Assessment of Diagnostic Accuracy Studies. After our data analysis, the sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic accuracy, summary receiver operating characteristic curve, funnel plot, box plot, and Fagan plot of the diagnostic method were determined. Nine articles with nine sets of data were finally included. The sensitivity and specificity were 0.94 and 0.98, respectively, the positive likelihood ratio was 79.53, the negative likelihood ratio was 0.05, the diagnostic odds ratio was 1590.32, and the summary receiver operating characteristic curve was 0.9958. The SD BIOLINE test has the advantages of high sensitivity, high specificity, low cost, and easy operation for diagnosing HCV. Therefore, we recommend using SD BIOLINE for rapid and effective screening of HCV, which is especially applicable for economically underdeveloped areas.","PeriodicalId":73374,"journal":{"name":"Infectious microbes & diseases","volume":"5 1","pages":"76 - 82"},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47595458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-29DOI: 10.1097/IM9.0000000000000116
Daniel Digwo, Chidinma P. Elebe, V. Chigor, Stella N. Madueke, Christian K. Ezeh, A. Ike
Abstract This review aimed to assess the occurrence of false-positive serological reaction between dengue and coronavirus disease 2019 (COVID-19) and its implications for diagnosis. Evidence syntheses were conducted by systematically reviewing available literature using multiple databases, including Web of Science, PubMed, Google Scholar and medRxiv. Among a total of 16 presented cases from clinical settings, cross-reaction to COVID-19 serological tests was observed in two (12.5%) dengue-positive patients, while 14 patients (87.5%) confirmed positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) showed a cross-reaction with dengue serological tests, leading to misdiagnosis and mismanagement by attending clinicians. Of 1789 SARS-CoV-2-positive sera, cross-reaction to dengue serological tests was observed in 180 sera (10%), which is higher than the cross-reaction observed for SARS-CoV-2 in archived pre-COVID-19 sera positive for a dengue infection (75 of 811, 9.2%, P = 0.674). Clinicians in tropical regions are therefore advised to interpret serological tests with caution and use a more pragmatic approach to triage these infections.
摘要本综述旨在评估登革热与2019冠状病毒病（新冠肺炎）之间假阳性血清学反应的发生及其对诊断的影响。证据综合是通过使用多个数据库系统审查现有文献进行的，包括Web of Science、PubMed、Google Scholar和medRxiv。在总共16例临床病例中，两名（12.5%）登革热阳性患者对新冠肺炎血清学检测呈交叉反应，14名（87.5%）确诊为严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）阳性的患者与登革热血清学检测呈交互反应，导致就诊临床医生误诊和管理不善。在1789份严重急性呼吸系统综合征冠状病毒2型阳性血清中，这高于在登革热感染的存档COVID-19前血清中观察到的SARS-CoV-2交叉反应（811份中的75份，9.2%，P=0.674）。因此，建议热带地区的临床医生谨慎解释血清学测试，并使用更实用的方法对这些感染进行分诊。
{"title":"Occurrence of False-Positive Tests and Cross-reactions Between COVID-19 and Dengue With Implications During Diagnosis: A Mixed Evidence Synthesis","authors":"Daniel Digwo, Chidinma P. Elebe, V. Chigor, Stella N. Madueke, Christian K. Ezeh, A. Ike","doi":"10.1097/IM9.0000000000000116","DOIUrl":"https://doi.org/10.1097/IM9.0000000000000116","url":null,"abstract":"Abstract This review aimed to assess the occurrence of false-positive serological reaction between dengue and coronavirus disease 2019 (COVID-19) and its implications for diagnosis. Evidence syntheses were conducted by systematically reviewing available literature using multiple databases, including Web of Science, PubMed, Google Scholar and medRxiv. Among a total of 16 presented cases from clinical settings, cross-reaction to COVID-19 serological tests was observed in two (12.5%) dengue-positive patients, while 14 patients (87.5%) confirmed positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) showed a cross-reaction with dengue serological tests, leading to misdiagnosis and mismanagement by attending clinicians. Of 1789 SARS-CoV-2-positive sera, cross-reaction to dengue serological tests was observed in 180 sera (10%), which is higher than the cross-reaction observed for SARS-CoV-2 in archived pre-COVID-19 sera positive for a dengue infection (75 of 811, 9.2%, P = 0.674). Clinicians in tropical regions are therefore advised to interpret serological tests with caution and use a more pragmatic approach to triage these infections.","PeriodicalId":73374,"journal":{"name":"Infectious microbes & diseases","volume":"42 17","pages":"64 - 75"},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41263470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-07DOI: 10.1097/IM9.0000000000000115
Tingting Li, Yuxian Xin, Dongxia Liu, Jingrong Sun, Jingwei Li, Y. Zhang, Jie Feng
Abstract Lyme disease (LD) is a tick-transmitted infection caused by Borrelia burgdorferi sensu lato species, which include B. burgdorferi, Borrelia afzelii and Borrelia garinii. The majority of patients with early LD can be cured by the standard treatment, yet some still suffer from posttreatment LD syndrome. The presence of Borrelia persisters has been proposed as a contributing factor, because they cannot be completely eradicated by the currently used antibiotics for LD. Finding new pharmaceuticals targeting Borrelia persisters is crucial for developing more effective treatments. Here, we first confirmed the existence of persisters in B. garinii and B. afzelii cultures and then conducted a high-throughput screening of a custom drug library against persister-rich stationary-phase B. garinii and B. afzelii cultures. Among 2427 compounds screened, hypocrellin A (HA), anthracycline class of drugs and topical antibiotics along with some other natural compounds were identified to have strong potential for killing persisters of B. garinii and B. afzelii. HA was the most active anti-Borrelia compound, capable of eradicating stationary-phase Borrelia persisters, in particular when combined with doxycycline and/or ceftriaxone. Liposoluble antioxidant vitamin E was found to antagonize the activity of HA, indicating HA’s target is the cell membrane where HA triggers the generation of reactive oxygen species in the presence of light. HA was found to have distinct bactericidal activity against Borrelia species but had poor or no activity against gram-positive and gram-negative bacteria. Identification of the abovementioned drug candidates may help develop more effective therapies for LD.
摘要莱姆病（LD）是由伯氏疏螺旋体（Borrelia burgdorferi sensu lato）引起的一种蜱传感染，包括伯氏芽孢杆菌（B.burgdorveri）、阿夫泽利疏螺旋体和加里尼疏螺旋体。大多数早期LD患者可以通过标准治疗治愈，但仍有一些患者患有治疗后LD综合征。持久性疏螺旋体的存在被认为是一个促成因素，因为目前用于LD的抗生素无法完全根除它们。寻找针对持久性疏螺旋体的新药对于开发更有效的治疗方法至关重要。在这里，我们首先确认了加里尼双歧杆菌和阿夫泽利双歧杆菌培养物中存在持久性蛋白酶，然后对针对富含持久性蛋白酶的加里尼芽孢杆菌和阿夫泽利芽孢杆菌培养物的固定相的定制药物库进行了高通量筛选。在筛选的2427个化合物中，竹霉素A（HA）、蒽环类药物和局部抗生素以及一些其他天然化合物被鉴定为具有很强的杀灭加里尼芽孢杆菌和阿夫泽利芽孢杆菌的潜力。HA是最有效的抗疏螺旋体化合物，能够根除固定相疏螺旋体持续存在，特别是与强力霉素和/或头孢曲松联合使用时。脂溶性抗氧化剂维生素E被发现能拮抗HA的活性，这表明HA的靶点是细胞膜，在光的存在下，HA在细胞膜上触发活性氧的产生。HA对疏螺旋体具有明显的杀菌活性，但对革兰氏阳性菌和革兰氏阴性菌的杀菌活性较差或没有。上述候选药物的鉴定可能有助于开发更有效的LD治疗方法。
{"title":"High-Throughput Drug Screening on Borrelia garinii and Borrelia afzelii Identified Hypocrellin A as an Active Drug Candidate Against Borrelia Species","authors":"Tingting Li, Yuxian Xin, Dongxia Liu, Jingrong Sun, Jingwei Li, Y. Zhang, Jie Feng","doi":"10.1097/IM9.0000000000000115","DOIUrl":"https://doi.org/10.1097/IM9.0000000000000115","url":null,"abstract":"Abstract Lyme disease (LD) is a tick-transmitted infection caused by Borrelia burgdorferi sensu lato species, which include B. burgdorferi, Borrelia afzelii and Borrelia garinii. The majority of patients with early LD can be cured by the standard treatment, yet some still suffer from posttreatment LD syndrome. The presence of Borrelia persisters has been proposed as a contributing factor, because they cannot be completely eradicated by the currently used antibiotics for LD. Finding new pharmaceuticals targeting Borrelia persisters is crucial for developing more effective treatments. Here, we first confirmed the existence of persisters in B. garinii and B. afzelii cultures and then conducted a high-throughput screening of a custom drug library against persister-rich stationary-phase B. garinii and B. afzelii cultures. Among 2427 compounds screened, hypocrellin A (HA), anthracycline class of drugs and topical antibiotics along with some other natural compounds were identified to have strong potential for killing persisters of B. garinii and B. afzelii. HA was the most active anti-Borrelia compound, capable of eradicating stationary-phase Borrelia persisters, in particular when combined with doxycycline and/or ceftriaxone. Liposoluble antioxidant vitamin E was found to antagonize the activity of HA, indicating HA’s target is the cell membrane where HA triggers the generation of reactive oxygen species in the presence of light. HA was found to have distinct bactericidal activity against Borrelia species but had poor or no activity against gram-positive and gram-negative bacteria. Identification of the abovementioned drug candidates may help develop more effective therapies for LD.","PeriodicalId":73374,"journal":{"name":"Infectious microbes & diseases","volume":"5 1","pages":"83 - 92"},"PeriodicalIF":0.0,"publicationDate":"2023-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46590696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-25DOI: 10.1097/im9.0000000000000112
In order to further strengthen the diagnosis and treatment of novel coronavirus infection (COVID-19), we revised the Diagnosis and Treatment Protocol for COVID-19 (Revised Trial Version 9) to Diagnosis and Treatment Protocol for COVID-19 (Trial Version 10). 1. Etiological characteristics Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the β-genus coronaviruses. It has an envelope, and the virus particle is round or oval, with a diameter ranging 60–140 nm. It contains four structural proteins, namely, the spike (S) protein, the envelope (E) protein, the matrix protein (M) and the nucleoprotein (N) protein. The genome of SARS-CoV-2 is a single-stranded positive-sense RNA molecule with a total length of approximately 29.9 kb. The open reading frames contained within the genome are arranged in sequence as 5′-replicase (ORF1a/ORF1b)-S-ORF3a-ORF3b-E-M-ORF6-ORF7a-ORF7b-ORF8-N-ORF9a-ORF9b-ORF10–3′. The RNA genome is wrapped by the N protein, forming a nucleocapsid, a core structure of the virus particle, surrounded by lipid bilayer membrane, in which the S protein, the M protein and the N protein of SARS-CoV-2 are embedded. After invading the human respiratory tract, SARS-CoV-2 mainly relies on the receptor binding domain of the S protein on the virus cell surface to recognize the host cell receptor angiotensin-converting enzyme 2, and then interacts with the receptor, allowing the virus to enter host cells. During the epidemic and transmission of SARS-CoV-2 in the population, its genes have frequently shown mutations. When different subvariants or lineages of SARS-CoV-2 infect the human body simultaneously, these viruses might recombine, resulting in the emergence of recombinant virus strains. Certain mutations or recombinations will affect the biological characteristics of the virus. For instance, mutations of specific amino acids on the S protein enhance the affinity between SARS-CoV-2 and angiotensin-converting enzyme 2, and concomitantly the ability of the virus to replicate and spread between cells. Some amino acid mutations on the S protein will also increase viral immune escape from vaccines and reduce the cross-protection between different subvariants, resulting in breakthrough infections and a certain proportion of reinfections. As of the end of 2022, five variants of concern, namely, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2) and Omicron (B.1.1.529), have been designated by the World Health Organization. Compared with other variants of concern such as Delta, the transmissibility and immune escape ability of the Omicron variant, which emerged in the population in November 2021, have been significantly enhanced and the Omicron variant has promptly replaced the Delta variant as the dominant global epidemic variant since early 2022. Up to now, the five subvariants of Omicron (BA.1, BA.2, BA.3, BA.4 and BA.5) have successively evolved into 709 serial lineages, including 72 recombinant lineages. As SARS-C
{"title":"Translation: Diagnosis and Treatment Protocol for COVID-19 (Trial Version 10)","authors":"","doi":"10.1097/im9.0000000000000112","DOIUrl":"https://doi.org/10.1097/im9.0000000000000112","url":null,"abstract":"In order to further strengthen the diagnosis and treatment of novel coronavirus infection (COVID-19), we revised the Diagnosis and Treatment Protocol for COVID-19 (Revised Trial Version 9) to Diagnosis and Treatment Protocol for COVID-19 (Trial Version 10). 1. Etiological characteristics Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the β-genus coronaviruses. It has an envelope, and the virus particle is round or oval, with a diameter ranging 60–140 nm. It contains four structural proteins, namely, the spike (S) protein, the envelope (E) protein, the matrix protein (M) and the nucleoprotein (N) protein. The genome of SARS-CoV-2 is a single-stranded positive-sense RNA molecule with a total length of approximately 29.9 kb. The open reading frames contained within the genome are arranged in sequence as 5′-replicase (ORF1a/ORF1b)-S-ORF3a-ORF3b-E-M-ORF6-ORF7a-ORF7b-ORF8-N-ORF9a-ORF9b-ORF10–3′. The RNA genome is wrapped by the N protein, forming a nucleocapsid, a core structure of the virus particle, surrounded by lipid bilayer membrane, in which the S protein, the M protein and the N protein of SARS-CoV-2 are embedded. After invading the human respiratory tract, SARS-CoV-2 mainly relies on the receptor binding domain of the S protein on the virus cell surface to recognize the host cell receptor angiotensin-converting enzyme 2, and then interacts with the receptor, allowing the virus to enter host cells. During the epidemic and transmission of SARS-CoV-2 in the population, its genes have frequently shown mutations. When different subvariants or lineages of SARS-CoV-2 infect the human body simultaneously, these viruses might recombine, resulting in the emergence of recombinant virus strains. Certain mutations or recombinations will affect the biological characteristics of the virus. For instance, mutations of specific amino acids on the S protein enhance the affinity between SARS-CoV-2 and angiotensin-converting enzyme 2, and concomitantly the ability of the virus to replicate and spread between cells. Some amino acid mutations on the S protein will also increase viral immune escape from vaccines and reduce the cross-protection between different subvariants, resulting in breakthrough infections and a certain proportion of reinfections. As of the end of 2022, five variants of concern, namely, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2) and Omicron (B.1.1.529), have been designated by the World Health Organization. Compared with other variants of concern such as Delta, the transmissibility and immune escape ability of the Omicron variant, which emerged in the population in November 2021, have been significantly enhanced and the Omicron variant has promptly replaced the Delta variant as the dominant global epidemic variant since early 2022. Up to now, the five subvariants of Omicron (BA.1, BA.2, BA.3, BA.4 and BA.5) have successively evolved into 709 serial lineages, including 72 recombinant lineages. As SARS-C","PeriodicalId":73374,"journal":{"name":"Infectious microbes & diseases","volume":"200 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135998469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-23DOI: 10.1097/IM9.0000000000000113
S. van der Veen
Abstract Neisseria gonorrhoeae is a multidrug-resistant bacterial pathogen for which ceftriaxone is the only remaining recommended first-line therapy. However, ceftriaxone susceptibility has been waning in a number of countries over the last decade and ceftriaxone treatment failures have been reported, commonly as a result of sporadic high-level ceftriaxone-resistant strains. In recent years, N. gonorrhoeae strains associated with the high-level ceftriaxone-resistant FC428 clone or strains that acquired its main ceftriaxone resistance determinant, penA allele 60.001, have shown global transmission, resulting in ceftriaxone treatment failure in a number of cases. The FC428 clone was first encountered in Japan in 2015 and subsequently in China, Europe, Australia, North America and Southeast Asia afterward. Strains associated with the FC428 clone commonly display a ceftriaxone minimum inhibitory concentration of 0.5–1 mg/L. However, where penA alleles encountered in sporadic high-level ceftriaxone-resistant isolates induce an in vitro growth defect, penA allele 60.001 does not seem to affect in vitro growth. The limited impact of penA allele 60.001 on biological fitness might be associated with its successful global transmission. Although the FC428 clone displays high-level ceftriaxone resistance, most gonorrhea cases associated with this clone were still successfully cured with ceftriaxone when intramuscular or intravenous doses of 500 mg to 2 g were used. A successful alternative therapy seems to be ertapenem given at 1-g doses, although further clinical studies are required to validate ertapenem efficacy. This review summarizes the global transmission of strains associated with the FC428 clone and antimicrobial treatment of associated cases.
{"title":"Global Transmission of the penA Allele 60.001–Containing High-Level Ceftriaxone-Resistant Gonococcal FC428 Clone and Antimicrobial Therapy of Associated Cases: A Review","authors":"S. van der Veen","doi":"10.1097/IM9.0000000000000113","DOIUrl":"https://doi.org/10.1097/IM9.0000000000000113","url":null,"abstract":"Abstract Neisseria gonorrhoeae is a multidrug-resistant bacterial pathogen for which ceftriaxone is the only remaining recommended first-line therapy. However, ceftriaxone susceptibility has been waning in a number of countries over the last decade and ceftriaxone treatment failures have been reported, commonly as a result of sporadic high-level ceftriaxone-resistant strains. In recent years, N. gonorrhoeae strains associated with the high-level ceftriaxone-resistant FC428 clone or strains that acquired its main ceftriaxone resistance determinant, penA allele 60.001, have shown global transmission, resulting in ceftriaxone treatment failure in a number of cases. The FC428 clone was first encountered in Japan in 2015 and subsequently in China, Europe, Australia, North America and Southeast Asia afterward. Strains associated with the FC428 clone commonly display a ceftriaxone minimum inhibitory concentration of 0.5–1 mg/L. However, where penA alleles encountered in sporadic high-level ceftriaxone-resistant isolates induce an in vitro growth defect, penA allele 60.001 does not seem to affect in vitro growth. The limited impact of penA allele 60.001 on biological fitness might be associated with its successful global transmission. Although the FC428 clone displays high-level ceftriaxone resistance, most gonorrhea cases associated with this clone were still successfully cured with ceftriaxone when intramuscular or intravenous doses of 500 mg to 2 g were used. A successful alternative therapy seems to be ertapenem given at 1-g doses, although further clinical studies are required to validate ertapenem efficacy. This review summarizes the global transmission of strains associated with the FC428 clone and antimicrobial treatment of associated cases.","PeriodicalId":73374,"journal":{"name":"Infectious microbes & diseases","volume":"5 1","pages":"13 - 20"},"PeriodicalIF":0.0,"publicationDate":"2023-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48742901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-14DOI: 10.1097/IM9.0000000000000111
Marcela Araujo de Oliveira Santana, S. Butt
Abstract Healthcare professionals in infectious diseases have been using Twitter to disseminate knowledge and interact with other professionals. This study aimed to identify and characterize the top 100 Twitter influencers in infectious diseases and to analyze the correlation between Twitter and academic influence. The Right Relevance Application Programming Interface was used to search for the term “infectious diseases,” producing a Twitter topic score for the top 100 infectious disease influencers. The influencers' Hirsch index (h-index) was compared with their Twitter topic score for a possible correlation. We identified that 46% of the infectious disease influencers were physicians. A correlation between the Twitter topic score of the infectious disease influencers and their h-index was not identified (r = 0.123, P = 0.22). The study also provides a list of the infectious disease influencers for those who have an interest in engaging and interacting with them on Twitter.
传染病领域的医疗保健专业人员一直在使用Twitter传播知识并与其他专业人员进行互动。本研究旨在识别和表征前100名Twitter传染病影响者，并分析Twitter与学术影响力之间的相关性。使用Right Relevance Application Programming Interface搜索“传染病”一词，生成100个传染病影响者的Twitter话题得分。影响者的赫希指数(h-index)与他们的推特话题得分进行了比较，以寻找可能的相关性。我们发现46%的传染病影响者是医生。传染病影响者的Twitter话题得分与其h指数之间没有相关性(r = 0.123, P = 0.22)。这项研究还为那些有兴趣在推特上与他们互动的人提供了一份传染病影响者名单。
{"title":"The Top 100 Twitter Influencers in Infectious Diseases","authors":"Marcela Araujo de Oliveira Santana, S. Butt","doi":"10.1097/IM9.0000000000000111","DOIUrl":"https://doi.org/10.1097/IM9.0000000000000111","url":null,"abstract":"Abstract Healthcare professionals in infectious diseases have been using Twitter to disseminate knowledge and interact with other professionals. This study aimed to identify and characterize the top 100 Twitter influencers in infectious diseases and to analyze the correlation between Twitter and academic influence. The Right Relevance Application Programming Interface was used to search for the term “infectious diseases,” producing a Twitter topic score for the top 100 infectious disease influencers. The influencers' Hirsch index (h-index) was compared with their Twitter topic score for a possible correlation. We identified that 46% of the infectious disease influencers were physicians. A correlation between the Twitter topic score of the infectious disease influencers and their h-index was not identified (r = 0.123, P = 0.22). The study also provides a list of the infectious disease influencers for those who have an interest in engaging and interacting with them on Twitter.","PeriodicalId":73374,"journal":{"name":"Infectious microbes & diseases","volume":"5 1","pages":"36 - 40"},"PeriodicalIF":0.0,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48208057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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