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Reproductive biology of Sphaeralcea species with ornamental interest 具有观赏价值的 Sphaeralcea 树种的生殖生物学
Q3 Agricultural and Biological Sciences Pub Date : 2024-01-18 DOI: 10.36253/ahsc-13616
Agustina Gutiérrez, Paula Monzón, Sandra Micheletto, Pablo Marinangeli
The genus Sphaeralcea belongs to the Malvaceae family and has native species from South America. Their attractive morphological characteristics with ornamental value have not yet been explored. The objective of this work was to know the viability of pollen, stigma receptivity, type of pollination and combining ability of four Sphaeralcea species (S. australis, S. bonariensis, S. crispa and S. mendocina), with the aim to develop new ornamental varieties. Fructification, fertility, seed germination and survival seedlings on intraspecific and reciprocal interspecific offspring were assessed. The highest values ​​of stigma receptivity and pollen viability were obtained at 2:00 pm for the four species. S. mendocina also showed high values ​​of pollen viability at 4:00 pm. The species proved to be self-incompatible and allogamous, with different degrees of reproductive compatibility. The interspecific crosses of S. mendocina and the intraspecific of S. crispa did not produce descendants. The crosses between S. australis and S. bonariensis as maternal parent presented the best combining ability with good fruit production, seed germination and survival. This research provides useful information for the formulation and implementation of breeding strategies, to improve pollination efficiency, and to breed new Sphaeralcea varieties with ornamental potential.
Sphaeralcea 属属于锦葵科,原产于南美洲。它们具有观赏价值的诱人形态特征尚未被发掘。这项工作的目的是了解四种 Sphaeralcea(S. australis、S. bonariensis、S. crispa 和 S. mendocina)的花粉活力、柱头接受能力、授粉类型和结合能力,以培育新的观赏品种。对种内和种间互交后代的果实、生育力、种子发芽率和幼苗存活率进行了评估。四个物种的柱头受精率和花粉存活率在下午 2:00 时达到最高值。S. mendocina 在下午 4:00 时的花粉活力值也很高。事实证明,这些物种自交不亲和,异花授粉,具有不同程度的生殖兼容性。S. mendocina 的种间杂交和 S. crispa 的种内杂交均未产生后代。以 S. australis 和 S. bonariensis 为母本的杂交表现出最佳的结合能力,果实产量、种子发芽率和存活率都很高。该研究为制定和实施育种策略、提高授粉效率以及培育具有观赏潜力的芒柄菊新品种提供了有用信息。
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引用次数: 0
Use of the biostimulant Retard Cherry® as a strategy to delay blooming period in sweet cherry trees 使用生物刺激剂 Retard Cherry® 作为推迟甜樱桃树开花期的策略
Q3 Agricultural and Biological Sciences Pub Date : 2024-01-18 DOI: 10.36253/ahsc-13659
J. A. Yuri, Miguel Palma, Álvaro Sepúlveda, J. Sánchez-Contreras, M. Moya
Spring frosts are a limiting factor in sweet cherry production in central-southern Chile. Sweet cherry trees cv. ‘Regina’ and ‘Sweetheart’ were studied to evaluate the effect of foliar application of a biostimulant (Retard Cherry®) prior to leaf fall on the bloom delay, fruit set, fruit drop, yield and quality. Data were compared to a non-product control. The study was conducted in the Maule Region, Chile. Results showed that the use of Retard Cherry® delayed full bloom by 6-8 days between cultivars compared to the control; however, there was no delay in the harvest date. The climatic conditions favored high fruit set (37%-49%) and low fruit drop (63%-70%) between cultivars in both treatments. Regarding fruit quality, no differences in size, soluble solids concentration and color were observed with the product, but a decrease in firmness were observed for ‘Regina’. These results show that Retard Cherry® is an effective tool in delaying bloom, providing trees with more favorable climatic conditions for pollination and fruit set.
春季霜冻是智利中南部甜樱桃生产的一个限制因素。研究人员对甜樱桃树变种 "Regina "和 "Sweetheart "进行了研究,以评估在落叶前叶面喷施生物刺激剂(Retard Cherry®)对开花延迟、坐果、落果、产量和质量的影响。数据与非产品对照进行了比较。这项研究在智利的毛莱地区进行。结果表明,与对照组相比,使用 Retard Cherry® 可使不同品种的盛花期推迟 6-8 天;但收获期没有推迟。气候条件有利于两种处理中不同品种的高坐果率(37%-49%)和低落果率(63%-70%)。在果实质量方面,产品在果实大小、可溶性固形物浓度和颜色方面均无差异,但 "Regina "的果实硬度有所下降。这些结果表明,Retard Cherry® 是延迟开花的有效工具,可为果树授粉和坐果提供更有利的气候条件。
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引用次数: 0
Yield related traits in some Persian walnut cultivars: Analysis of genetic and genetic by environment interaction 一些波斯核桃栽培品种的产量相关性状:遗传和遗传与环境相互作用的分析
Q3 Agricultural and Biological Sciences Pub Date : 2024-01-18 DOI: 10.36253/ahsc-13749
A. Soleimani, V. Rabiei, D. Hassani, M. Mozaffari, R. Dastjerdi
The most important trait in tree species, including walnut, is the yield. In this study, the effect of genotype and their interaction with year on Nut weight, Kernel weight, Kernel percentage, Fruit set, Nuts number on Scaffold (Canopy) Cross Area (SCA), Nut weight on SCA and Kernel weight on SCA were evaluated on Caspian, Persia, Alvand, and Chaldoran walnut cultivars. The results showed that the effects of year, genotype, and year × genotype interaction on all traits were significant. The results showed that Alvand had the highest number of nuts (41.8 per m2) and nut weight (472.1 g/m2) on (SCA). Heritability (H2b) for kernel weight and kernel percentage, were estimated 0.75 and 0.80, respectively. The lowest value of H2b (0.36) was belong to fruit set. The analyses of genetic and phenotypic correlations between traits showed that, the nut weight had (rg = 0.31, rp = 0.27) a moderate correlation with SCA same as kernel weight (rg = 0.34, rp = 0.29). The GGE biplot analysis explained most of the existing variations (>90%). The genetic effect (PC1) for all traits were higher respect to the genetic × environment interaction (PC2), especially for the kernel percentage (94.4%) and number and weight of nut and kernel on SCA (>90%). The lowest value of the PC1 was related to the fruit set (65.6%), which indicates the trait was more affected by genetic × environment interactions (21.8%). So, this result showed that the yield-related traits in walnut is highly relevant to environment(year in this study) and evaluation of the new cultivars needs careful attention in this case.
核桃等树种最重要的性状是产量。本研究评估了基因型及其与年份的交互作用对 Caspian、Persia、Alvand 和 Chaldoran 核桃栽培品种的坚果重量、核仁重量、核仁率、坐果率、棚架(冠层)横截面积(SCA)上的坚果数量、SCA 上的坚果重量和 SCA 上的核仁重量的影响。结果表明,年份、基因型和年份×基因型交互作用对所有性状的影响都很显著。结果表明,Alvand 的坚果数(41.8 个/平方米)和坚果重(472.1 克/平方米)在(SCA)上最高。果仁重量和果仁率的遗传力(H2b)估计分别为 0.75 和 0.80。坐果率的遗传力 H2b 值最低(0.36)。性状间的遗传和表型相关性分析表明,坚果重(rg = 0.31,rp = 0.27)与 SCA 的相关性中等,与果仁重(rg = 0.34,rp = 0.29)的相关性相同。GGE 双图分析解释了大部分现有变异(大于 90%)。所有性状的遗传效应(PC1)均高于遗传 × 环境交互作用(PC2),尤其是果仁率(94.4%)以及坚果和果仁的数量和重量对 SCA 的影响(>90%)。PC1 值最低的是坐果率(65.6%),表明该性状受遗传 × 环境交互作用的影响较大(21.8%)。因此,这一结果表明核桃的产量相关性状与环境(本研究中的年份)高度相关,在这种情况下评估新的栽培品种需要谨慎。
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引用次数: 0
Evaluation of viability and germination of pollen grains of three local caprifig cultivars and their effect on some characteristics of fig fruits (Ficus carica L.) 评估三种本地无花果栽培品种花粉粒的活力和发芽率及其对无花果果实(Ficus carica L.)某些特征的影响
Q3 Agricultural and Biological Sciences Pub Date : 2024-01-18 DOI: 10.36253/ahsc-14098
Hafsa Barakat, Rida Draie
This research was carried out in fig fields in the village of Kafar-Jales, in the north of Syria. The analyses were conducted in the laboratories of the Faculty of Agriculture at Idlib University during the 2022 farming season, to evaluate the viability and germination of three local varieties of Caprifig (Bunduqi, Azraq, Panjani). The morphological characteristics, the date of the exodus of insects (Blastophaga psenes) from male fruits, the quantity of pollen, and the percentage of germination and viability were studied on four nutritive media at a temperature of 27°C. The measurements were taken after 24 h, 48 h, and 72 h. These three pollinators were used to pollinate three varieties of edible figs (white Satehi, Safrawi, and Habashi) to study the effect of pollen source on the productive characteristics of edible fig fruits. The result showed significant superiority of the Bunduqi cultivar over the rest of the cultivars in traits of the fruit’s early ripening, pollen quantity, and date of departure of insects. The Azraq cultivar is found to be superior to Bunduqi and Panjani cultivars in the germination rate of pollen at all stages of the experiment and in all used media. The addition of stigma flower extract in culture media increased germination percentage by 35% for all studied Caprifig cultivars. The addition of 10% sucrose in the cultivation environment increased the percentage of pollen germination by 20%. The cultivars that were inoculated with the Panjani pollinator outperformed the characteristics of the length, diameter, and weight, demonstrating that the fruit’s quality characteristics are affected by the genotype of the pollinator used. The results of this research can be beneficial for both fig growers and plant breeders, as they help to select the best pollinators and contribute to the development and improvement of the quality of the cultivated fig fruits.
这项研究是在叙利亚北部 Kafar-Jales 村的无花果田里进行的。分析是在伊德利卜大学农学院实验室进行的,时间是 2022 年农忙季节,目的是评估三个当地无花果品种(Bunduqi、Azraq 和 Panjani)的存活率和发芽率。在 27°C 的温度下,在四种营养介质上研究了雄果的形态特征、昆虫(Blastophaga psenes)出壳日期、花粉数量、发芽率和存活率。利用这三种授粉昆虫为三个品种的食用无花果(白萨特希、萨弗拉维和哈巴什)授粉,以研究花粉源对食用无花果果实产量特性的影响。结果表明,Bunduqi 栽培品种在果实早熟、花粉量和昆虫离去日期等性状上明显优于其他栽培品种。在实验的所有阶段和所有使用的培养基中,Azraq 栽培品种的花粉发芽率均优于 Bunduqi 和 Panjani 栽培品种。在培养基中添加柱头花提取物可使所有研究的 Caprifig 栽培品种的发芽率提高 35%。在培养环境中添加 10%的蔗糖可使花粉发芽率提高 20%。接种了 Panjani 授粉器的栽培品种在果实长度、直径和重量等特性方面表现优异,这表明果实的质量特性受所用授粉器基因型的影响。这项研究的结果对无花果种植者和植物育种者都有益处,因为它们有助于选择最佳授粉者,促进无花果栽培果实品质的发展和提高。
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引用次数: 0
Warm stratification combined with organic manure application enhances seed germination and improves Cycas revoluta growth and development 温暖层积与施用有机肥相结合,可提高种子萌发率,改善苏铁的生长发育
Q3 Agricultural and Biological Sciences Pub Date : 2024-01-18 DOI: 10.36253/ahsc-14544
J. Benjelloun, Sarah Bouzroud, Y. Zaid, A. Guedira, A. Smouni
Cycas revoluta (Sago palm) is one of the widespread ornamental plant, used as an indoor and outdoor plant. Seed propagation is extremely hard and time consuming, given the physical dormancy imposed by hard coat. The use of warm stratification improves seed germination by prompting embryos development. As mean to gain more insight on the beneficial effect of warm treatment on seed germination, histological analysis of warm stratified and untreated embryos was conducted. Our results revealed that warm treatment accelerated embryos development, resulting in a rapid differentiation of embryos’ tissues. α-amylase, GA3 and ABA quantification showed that warm stratified embryos accumulated higher and lower amounts of α-amylase and ABA respectively compared to untreated embryos. Regarding plant development, our results showed that organic manures significantly improved Cycas revoluta growth and development. The best response was recorded with the application of sheep manure. Indeed, sheep manure addition increased plant height, the number of leaves per plant, stip length and width by nearby 188% and 61%, 36% and 17% respectively. In roots, the presence of nodules had been recorded in the three applied treatments and more importantly in the presence of sheep manure. At the physiological level, sheep manure supplementation improved photosynthetic apparatus and nitrogen content in leaves (by 75%), thereby explaining the growth promotion. Taken together, these results underlined the beneficial effect of organic manure on Cycas revoluta growth and development and proposed a new strategy to improve plant growth and development with the use of sheep manure as organic amendment.
苏铁(西米棕榈)是一种广泛种植的观赏植物,可用作室内和室外植物。由于硬皮层的物理休眠作用,种子繁殖非常困难且耗时。采用温暖层积法可以促进胚胎发育,从而提高种子发芽率。为了更深入地了解温育对种子萌发的有利影响,我们对温育和未温育的胚胎进行了组织学分析。α-淀粉酶、GA3 和 ABA 的定量分析表明,与未处理的胚胎相比,温分层胚胎积累的 α-淀粉酶和 ABA 分别较高和较低。在植物发育方面,我们的研究结果表明,有机肥能显著改善苏铁的生长和发育。施用羊粪的效果最好。事实上,添加羊粪后,植株高度、单株叶片数、规定长度和宽度分别增加了 188%、61%、36% 和 17%。在根部,三种施用处理都记录到了结节的存在,更重要的是在有羊粪存在的情况下。在生理层面上,补充羊粪改善了叶片中的光合装置和氮含量(75%),从而解释了促进生长的原因。综上所述,这些结果凸显了有机肥对苏铁生长和发育的有利影响,并提出了一种利用羊粪作为有机肥改善植物生长和发育的新策略。
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引用次数: 0
Yield performance and nutritional quality of tomato hybrids in response to protected environments during the Amazonian summer 亚马逊夏季番茄杂交种的产量表现和营养质量对保护环境的反应
Q3 Agricultural and Biological Sciences Pub Date : 2024-01-18 DOI: 10.36253/ahsc-13558
Vicente Pataraico Junior, Flávio Fernandes Junior, Marcio Roggia Zanuzo, Rene Arnoux Da Silva Campos, Franciely da Silva Ponce, Silvia De Carvalho Campos Botelho, Ivone Vieira da Silva, D. T. Antunes, Maria Shirlyane Pereira do Nascimento, Santino Seabra Júnior
This study evaluated the yield performance of the tomato hybrids 'DS0060', 'Thaise' and 'Trucker' in the open field and environments protected by agricultural film (F) and polycarbonate panels (P) during the Amazonian summer. In the protected environment, the crops produced significantly higher yields than in the open field. 'Thaise' has high thermotolerance and is adaptable to a wide temperature range, making it the best-performing hybrid in environment F. Highest yields were found for 'Thaise' in environment F or P (86.2 and 92.5 t ha-1) together with 'DS0060' and 'Trucker' in environment F (75.3 and 88.2 t ha-1), demonstrating the high yield potential in the interim growing season (January to April). In the open field, the fruit color was paler, fruit flesh firmer and ripening index lower. In environment F, the fruits contained highest levels of soluble solids, lycopene and β-carotene. 'Thaise' contained higher concentrations of these two compounds. Under environment P, the yield of the evaluated tomato hybrids increased considerably, indicating it as a promising possibility for tomato cultivation in tropical regions. 'Thaíse' stood out with high yield and good quality traits, when grown in an F or P environment. These results prove the viability of tomato production as interim crop in tropical regions, under high rainfall and heat, as well as the difference protected environments make for tomato cultivation, in particular the choice of the most suitable cover material for the crop, to ensure high yields coupled with desirable quality properties.
这项研究评估了番茄杂交种 "DS0060"、"Thaise "和 "Trucker "在亚马逊夏季露地以及农用薄膜(F)和聚碳酸酯板(P)保护环境中的产量表现。在受保护的环境中,作物的产量明显高于露地。在 F 或 P 环境中,'Thaise'的产量最高(86.2 吨/公顷-1 和 92.5 吨/公顷-1),在 F 环境中,'DS0060'和'Trucker'的产量也最高(75.3 吨/公顷-1 和 88.2 吨/公顷-1),这表明在中期生长季节(1 月至 4 月),'Thaise'具有很高的产量潜力。在露地,果实颜色较浅,果肉较硬,成熟指数较低。在 F 环境中,果实的可溶性固形物、番茄红素和 β-胡萝卜素含量最高。Thaise "含有更高浓度的这两种化合物。在 P 环境下,所评价的番茄杂交种的产量显著增加,这表明在热带地区种植番茄很有前景。在 F 或 P 环境下,'Thaíse'产量高、品质好。这些结果证明了番茄作为热带地区的临时作物,在高降雨量和高温条件下生产的可行性,以及保护性环境对番茄栽培的影响,特别是为作物选择最合适的覆盖材料,以确保高产和理想的品质特性。
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引用次数: 0
Shelf-life and post-harvest quality of tomato (Lycopesicon esculentum Mill.) varieties to different packaging materials at Mersa, North Wollo, Ethiopia 埃塞俄比亚北沃洛梅尔萨地区番茄(Lycopesicon esculentum Mill.)
Q3 Agricultural and Biological Sciences Pub Date : 2024-01-18 DOI: 10.36253/ahsc-14410
S. Muhie, S. Worku, B. Masrie
Tomato (Lycopersicon esculentum Mill.) has a short shelf life at ambient conditions and is a highly perishable crop. Extreme post-harvest losses occur as a result of the wrong packaging materials. However, by employing the right packing materials, tomato varietals can have longer shelf lives. Globally rising fresh tomato demand has forced the development of essential mechanisms, including packaging materials, to improve shelf life. The current study was initiated to evaluate the quality and shelf-life of tomato varieties in response to packaging materials at Mersa, North Wollo, Ethiopia, during 2021 cropping season. Three replications of a completely randomized design were used to test three tomato varieties (Roma VF, Oval red and Woyno) and seven packing materials [closed carton (CC), open carton (OC), closed wooden box (CWB), open wooden box (OWB), perforated polyethylene bag (PPB), non-perforated polyethylene bag (NPPB) and control (C)] at room temperature (20-22°C). According to the findings, there is a significant (P<0.05) interaction effect between packaging materials and varieties on a number of parameters, including physiological weight loss, decay percentage, disease incidence, total soluble solids (TSS), tomato fruit PH, juice color score, overall acceptability, marketability percentage, and shelf life. Non-perforated polyethylene plastic experienced the highest physiological weight losses of 79.88% and 79.63% after 18 days of storage. Roma VF variety showed the greatest weight loss. In addition, PPB showed the lowest decay percentage (20%) and maximum marketability (20%) during the 18th day of storage. At the end of storage, NPPB with Roma VF and Woyno varieties had a substantially (100%) larger decay loss of tomato fruits. NPPB has been linked to the highest disease incidence (20%). Roma VF and Oval red recorded the highest pH tomato fruit’s color and overall acceptability score on PPB. It can, thus, be concluded that packaging of tomato fruits in PPB can extend shelf-life with better-quality of the produce. However, to develop plausible recommendation, the study should be repeated in multi-location with more packaging methods and varieties over seasons.
番茄(Lycopersicon esculentum Mill.)错误的包装材料会造成严重的收获后损失。然而,通过使用正确的包装材料,番茄品种的货架期可以延长。全球对新鲜番茄的需求不断增长,迫使人们开发包括包装材料在内的必要机制,以提高保质期。本研究旨在评估埃塞俄比亚北沃洛梅尔萨地区 2021 年种植季节番茄品种的质量和货架期对包装材料的影响。采用完全随机设计的三次重复,在室温(20-22°C)下对三个番茄品种(Roma VF、Oval red 和 Woyno)和七种包装材料[封闭纸箱 (CC)、开放纸箱 (OC)、封闭木箱 (CWB)、开放木箱 (OWB)、带孔聚乙烯袋 (PPB)、无孔聚乙烯袋 (NPPB) 和对照 (C)]进行了测试。研究结果表明,包装材料和品种之间存在着显著的交互作用(P<0.05),这些交互作用影响了多项参数,包括生理失重、腐烂率、病害发生率、总可溶性固形物(TSS)、番茄果实 PH 值、果汁颜色得分、总体可接受性、适销率和货架期。贮藏 18 天后,无孔聚乙烯塑料的生理失重率最高,分别为 79.88% 和 79.63%。罗马 VF 品种的重量损失最大。此外,在储存的第 18 天,PPB 的腐烂率最低(20%),上市率最高(20%)。贮藏结束时,使用 Roma VF 和 Woyno 品种的 NPPB 番茄果实的腐烂率大幅增加(100%)。NPPB 与最高的病害发生率(20%)有关。在 PPB 中,Roma VF 和 Oval red 的番茄果实颜色 pH 值和总体可接受性得分最高。因此,可以得出结论,用 PPB 包装番茄果实可以延长货架期,提高产品的质量。不过,为了提出合理的建议,这项研究应在多个地点重复进行,并在不同季节采用更多的包装方法和品种。
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引用次数: 0
Potassium silicate enhances drought tolerance of Bellis perennis by improving antioxidant activity and osmotic regulators 硅酸钾通过提高抗氧化活性和渗透调节因子增强铃兰的耐旱性
Q3 Agricultural and Biological Sciences Pub Date : 2024-01-18 DOI: 10.36253/ahsc-14732
A. Oraee, A. Tehranifar, Zahra Ghorbani, Pegah Sayad-Amin
Ornamental plants can usually encounter various types of environmental stress, which reduce plant productivity. A proper application of fertilizers can improve plantsʼ tolerance to drought stress. Nutrients such as potassium and silicon are known to have beneficial effects. This study aimed to evaluate the growth of Bellis perennis under drought stress (80, 70, and 60% FC) and with the application of potassium silicate (0, 2, and 4 mM). The results showed that potassium silicate (2 and 4 mM) increased K and Si accumulation in plants under drought stress. Plants treated with potassium silicate under drought stress exhibited a lower degree of electrolyte leakage and less MDA accumulation in the following order: 2 and 4 mM potassium silicate. An increase in relative water content and chlorophyll was observed with application of potassium silicate under drought stress. Regardless of potassium silicate, the plant enzymatic defense system was significantly improved compared to non-stressed plants. Potassium silicate enhanced the amount of osmotic regulators (carbohydrate and proline) and secondary metabolites (flavonoids and phenols) compared to control plants regardless of drought stress. The anthocyanin content in the flowers significantly decreased by 32.2% when the plants were treated with 4 mM potassium silicate at 60% FC, compared to 80% FC. In conclusion, potassium silicate mitigated the effects of drought stress, enhanced plant tolerance to drought stress, increased the activity of antioxidant enzymes, and improved the amounts of osmotic regulators and secondary metabolites.
观赏植物通常会遇到各种环境压力,从而降低植物的产量。适当施肥可提高植物对干旱胁迫的耐受力。众所周知,钾和硅等营养元素具有有益的作用。本研究旨在评估在干旱胁迫(80%、70% 和 60% FC)和施用硅酸钾(0、2 和 4 毫摩尔)条件下铃兰的生长情况。结果表明,在干旱胁迫下,硅酸钾(2 毫摩尔和 4 毫摩尔)增加了植物体内钾和硅的积累。在干旱胁迫下,使用硅酸钾处理的植物电解质渗漏程度较低,MDA积累也较少,依次为2毫摩尔和4毫摩尔硅酸钾。在干旱胁迫下施用硅酸钾后,相对含水量和叶绿素都有所增加。与未受胁迫的植物相比,无论施用哪种硅酸钾,植物酶防御系统都有明显改善。与对照植物相比,无论干旱胁迫与否,硅酸钾都能提高渗透调节剂(碳水化合物和脯氨酸)和次生代谢物(类黄酮和酚类)的含量。与 80% FC 相比,在 60% FC 条件下用 4 mM 硅酸钾处理植株时,花朵中的花青素含量显著减少了 32.2%。总之,硅酸钾能减轻干旱胁迫的影响,增强植物对干旱胁迫的耐受性,提高抗氧化酶的活性,改善渗透调节剂和次生代谢物的含量。
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引用次数: 0
In vitro propagation and microtuberization of potato (Solanum tuberosum L.) Spunta variety in Lebanon 黎巴嫩马铃薯(Solanum tuberosum L.)Spunta品种的体外繁殖和微茎化
Q3 Agricultural and Biological Sciences Pub Date : 2023-11-22 DOI: 10.36253/ahsc-13895
M. Dalleh, J. Borjac, G. Younes, E. Choueiri, A. Chehade, A. Elbitar
One of the factors that causes low productivity of potatoes in Lebanon is the limited availability of certified seeds. The aim of this study was to establish a rapid protocol for in vitro propagation and microtuberization of potato (Solanum tuberosum L.) of Spunta variety. Meristems culture associated to thermotherapy (one month/37°C) constituted the first step. The highest percentage of reactive meristem (92%) was observed on MS medium devoid of growth regulators while MS medium containing Kin 0.4 mg.l-1, GA3 0.5 mg.l-1 and IBA 0.5 mg.l-1 yielded the highest average number of shootlets (7.8) in the seventh subculture. The lowest number of days obtained for microtuber formation was 10 and the highest average number of microtuber (1.49) was obtained with shootlets incubated under C2 culture conditions (16-h day/8-h night for initial 7 days at 25±2°C; for remaining period: continuous dark at 17±2°C). Contrary the highest microtubers average length (10.75 mm), average width (7.41 mm) and average weight (646.26 mg) were produced under C1 culture conditions (16-h day/8-h night at 25±2°C). Medium supplemented with 5 mg.l-1 BAP and 6% sucrose presented the highest average number of microtubers of 2.36 and 1.94 respectively. Type and concentration of cytokines and sucrose concentration did not have significant effect on the average length, width and weight of microtubers produced.
黎巴嫩马铃薯产量低的原因之一是认证种子的供应有限。本研究的目的是为 Spunta 品种马铃薯(Solanum tuberosum L.)的体外繁殖和微突建立一个快速方案。第一步是进行与热疗(一个月/37°C)相关的分生组织培养。在不含生长调节剂的 MS 培养基上观察到的活性分生组织比例最高(92%),而在含 Kin 0.4 mg.l-1、GA3 0.5 mg.l-1 和 IBA 0.5 mg.l-1 的 MS 培养基上,第七次亚培养产生的小芽平均数量最多(7.8)。微管形成的最低天数为 10 天,而在 C2 培养条件(最初 7 天在 25±2°C 下昼夜各 16 小时;其余时间:在 17±2°C 下持续黑暗培养)下培养的小芽平均微管数最高(1.49)。相反,在 C1 培养条件下(25±2°C,昼夜各 16 小时)产生的微管平均长度(10.75 毫米)、平均宽度(7.41 毫米)和平均重量(646.26 毫克)最高。添加 5 mg.l-1 BAP 和 6% 蔗糖的培养基产生的微管平均数量最多,分别为 2.36 和 1.94。细胞因子的种类和浓度以及蔗糖浓度对产生的微管的平均长度、宽度和重量没有显著影响。
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引用次数: 0
Response of hydroponic baby lettuce to UV-B radiation exposure during the growing period 水培小莴苣对生长期紫外线-B 辐射照射的反应
Q3 Agricultural and Biological Sciences Pub Date : 2023-11-22 DOI: 10.36253/ahsc-13849
Ana Cecilia Silveira Gómez, Luis Rivera Marchant, Victor Hugo Escalona Contreras
Lettuce (Lactuca sativa L.) has a nutritional contribution comparable to other vegetables. It is produced in soil and hydroponics systems, outdoors or indoors, and in some cases, with the management of radiation. UV-B radiation exposure can influence the functional quality of vegetables and is becoming more frequent. Cultivars Kristine RZ and Versaï RZ were exposed to four radiation doses: UV-B0 (0 μW·cm-2), UV-B16 (16 μW·cm-2), UV -B33 (33 μW·cm-2) and UV-B58 (58 μW·cm-2), during 30 min for 10 days. Lettuce leaves were harvested twice. The leaf area of 'Versaï RZ' was not affected by radiation in the first harvest, while the high doses (33 and 58 µW·cm-2) reduced the leaf area of 'Kristine RZ' between 15-30 %, respectively. The radiation did not significantly impact the percentage of dry matter and the color parameters. However, functional compounds were affected. In general, the cv. Kristine RZ responded positively to the dose of 16 µW·cm-2 while 'Versaï RZ' to 58 µW·cm-2. An increase in the content of functional compounds was also observed in 'Versaï RZ' in the second harvest, and a reduction in the levels measured in 'Kristine RZ' indicated a different adaptation to UV-B radiation that must be studied individually.
生菜(Lactuca sativa L.)的营养价值可与其他蔬菜媲美。它可以在室外或室内的土壤和水培系统中生产,在某些情况下,还可以在辐射管理下生产。紫外线-B 辐射会影响蔬菜的功能质量,而且越来越频繁。Kristine RZ 和 Versaï RZ 两个品种受到了四种辐射剂量的照射:紫外线-B0(0 μW-cm-2)、紫外线-B16(16 μW-cm-2)、紫外线-B33(33 μW-cm-2)和紫外线-B58(58 μW-cm-2),持续时间为 30 分钟,为期 10 天。生菜叶片收割两次。第一次收获时,"Versaï RZ "的叶面积未受辐射影响,而高剂量(33 和 58 μW-cm-2)则使 "Kristine RZ "的叶面积分别减少了 15-30 %。辐射对干物质百分比和颜色参数没有明显影响。不过,功能化合物受到了影响。总的来说,'Kristine RZ'品种对辐射有积极的反应。Kristine RZ "对 16 µW-cm-2 的剂量反应积极,而 "Versaï RZ "对 58 µW-cm-2 的剂量反应积极。在第二次收获时,还观察到 "Versaï RZ "的功能化合物含量有所增加,而 "Kristine RZ "的含量则有所下降,这表明其对紫外线-B 辐射的适应性不同,必须进行单独研究。
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Advances in horticultural science
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