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SARS-CoV-2 Detection: Fast and Cost-Effective Sample Processing Prior to RT-PCR SARS-CoV-2检测:RT-PCR前快速和经济有效的样品处理
Pub Date : 2021-06-04 DOI: 10.11648/J.IJBECS.20210702.13
C. Salvador-García, Maria Dolores Ocete-Mochon, Rafael Medina-Gonzalez, Begoña Fuster-Escrivá, Sonia Cortes-Badenes, Maria Carmen Breso-Vila, Maria Jose Lahiguera-Abalos, C. Gimeno-Cardona
The pandemic COVID-19 needs a rapid microbiological diagnostic from Clinical Microbiology Units. Due to the fact that it is done by using a reverse transcription polymerase chain reaction (rRT-PCR) previous RNA extraction and automated equipment and reagents for RNA extraction represent an economic increase to the diagnosis, we describe an easy, cost-effective and fast alternative extraction-free SARS-CoV-2. Samples were treated with proteinase K for 10 minutes at 55°C. Then, there is a heat-process for 5 minutes at 98°C and finally, 3 minutes at -20°C before a commercial-commonly-used rRT-PCR procedure. The RNA automated-extraction was also performed with QIAsymphony RNA Kit (Qiagen) equipment. A total of 220 nasopharyngeal and oropharyngeal swabs were analyzed. 113 samples were tested positive whereas 106 samples were tested negative with RNA automated-extraction and extraction-free method, for an agreement of 99%. A total of one discordant sample was noted in which no amplified result (gene ORF1ab and N) were observed by RNA automated-extraction and gene ORF1ab (Ct 39) and gene N (Ct 37) by extraction-free. Thus, results were comparable with automated-extraction. This method is not only clinically acceptable but also confers an easy, fast, and cost-effective alternative to automated-extraction. Therefore, microbiological laboratories, with low economics resources and/or without automated-extraction equipment, could incorporate it.
COVID-19大流行需要临床微生物学单位的快速微生物学诊断。由于它是通过使用逆转录聚合酶链反应(rRT-PCR)之前的RNA提取和RNA提取的自动化设备和试剂来完成的,这代表了诊断的经济增长,我们描述了一种简单,经济且快速的替代无提取的SARS-CoV-2。样品用蛋白酶K在55℃下处理10分钟。然后,在98°C下加热5分钟,最后在-20°C下加热3分钟,然后进行商业常用的rRT-PCR程序。采用QIAsymphony RNA Kit (Qiagen)设备进行RNA自动提取。对220份鼻咽拭子和口咽拭子进行分析。RNA自动提取法和无提取法检测阳性113份,阴性106份,一致性为99%。共有1份不一致样本,RNA自动提取未观察到扩增结果(ORF1ab基因和N基因),无提取ORF1ab基因(Ct 39)和N基因(Ct 37)。因此,结果与自动提取具有可比性。该方法不仅在临床上可接受,而且为自动提取提供了一种简单、快速、经济的替代方法。因此,经济资源低和/或没有自动提取设备的微生物实验室可以采用它。
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引用次数: 0
Clinical Competence and Associated Factors Among Nurses working in Selected Health Institutions of Ilu AbaBor Zone, South-West Ethiopia: A Cross Sectional Study 埃塞俄比亚西南部Ilu AbaBor地区选定卫生机构护士临床能力及相关因素的横断面研究
Pub Date : 2021-04-20 DOI: 10.11648/J.IJBECS.20210702.11
Bonsa Amsalu Geleta, Sanbato Tamiru Dingata, Milkias Dugassa Emanu
Introduction: Clinical competence is fundamental element in the provision of nursing care and now a day it is the concern and the centre of attention for managers and the healthcare systems. Higher level of clinical competence has a positive impact on patient’s health outcome and nurse’s job performance and satisfaction. However, there is limited information on clinical competence of nurse in Ethiopia. Objective: The objective of this study was to assess clinical competency and associated factors among nurses working in selected health institutions of Illubabor zone, oromia regional state, north-west Ethiopia, 2019. Methods: Institution based cross-sectional study was employed on 160 nurses in two hospitals and 20 health centres. Simple random sampling technique was employed to select study participants. Structured self-administered questionnaire was used to collect data. Independent t-test and analysis of variance (ANOVA) were used to identify factors associated with clinical competence. The statistical significance was set at p<0.05. Results: Out 160 recruited, 156 participants were responded the questionnaire, making the response rate of 97.5%. The overall clinical competence of participants was 2.23 (SD=0.6) which indicates moderate level of clinical competence. Participants had higher competence score on Legal/ethical dimension and lower competence score on teaching coaching dimension. Age, marital status, level of education, work experience, type of health facility, average income, and current position, retrieval of newly published information, previous training, and frequency of trainings, having guideline/manual and using guideline/manual currently were the identified factors association with clinical competence of nurses. Conclusion: In the current rapidly changing healthcare environment, the need for clinical competence among nurses is continually increasing. However, clinical competence of nurses in the current study was inadequate in which the overall score of participants was almost only half of total score. It is recommended that health policy makers should set strategies to assess the clinical competence of nurses on a periodic basis in order to assure quality nursing service.
简介:临床能力是提供护理的基本要素,现在它是管理者和医疗保健系统关注和关注的中心。较高的临床能力水平对患者的健康结局、护士的工作绩效和满意度均有正向影响。然而,关于埃塞俄比亚护士临床能力的信息有限。目的:本研究的目的是评估2019年在埃塞俄比亚西北部奥罗米亚州Illubabor地区选定卫生机构工作的护士的临床能力及其相关因素。方法:采用基于机构的横断面研究,对两所医院和20个保健中心的160名护士进行调查。采用简单随机抽样方法选择研究对象。采用结构化自我管理问卷收集数据。采用独立t检验和方差分析(ANOVA)确定与临床能力相关的因素。p<0.05为差异有统计学意义。结果:160名参与者中,156人完成问卷调查,问卷回复率为97.5%。被试的整体临床能力为2.23 (SD=0.6),为中等水平。参与者在法律/伦理维度上的胜任力得分较高,在教学辅导维度上的胜任力得分较低。年龄、婚姻状况、受教育程度、工作经验、卫生机构类型、平均收入、当前职位、新发布信息检索、既往培训、培训频率、是否拥有指南/手册和目前使用指南/手册是影响护士临床能力的因素。结论:在当前瞬息万变的医疗环境中,对护士临床能力的要求不断提高。然而,目前的研究中,护士的临床能力不足,参与者的综合得分几乎只有总分的一半。建议卫生决策者应制定策略,定期评估护士的临床能力,以确保优质的护理服务。
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引用次数: 1
Prevalence of Stillbirth and Associated Factors among Immediate Postpartum Mothers at Bahir Dar, Felegehiwot Hospital, Northwest Ethiopia: Cross-sectional Study 埃塞俄比亚西北部Felegehiwot医院Bahir Dar的产后母亲死产发生率及相关因素:横断面研究
Pub Date : 2021-04-20 DOI: 10.11648/J.IJBECS.20210702.12
Agerie Mengistie Zeleke, M. Asemahagn
Background: Stillbirth rates are among the indicators of maternal and child health care quality in the countries. However, the majority of neonatal deaths and almost all stillbirths were underreported in Ethiopia. Therefore, this study aimed to determine the prevalence of stillbirth and associated factors among immediate postpartum mothers in the study area. Methods: An institution-based cross-sectional study was employed at Felegehiwot comprehensive specialized hospital from March to May 2016 (n=310). A pretested structured interviewer- administered questionnaire and medical chart reviews were used to collect data from immediate postpartum mothers. Data were entered into Epi Info version 7 and analyzed using SPSS version 20. The binary logistic regression model fitted to identify stillbirth; adjusted odds ratio (AOR) with a 95% confidence interval (CI) was computed to assess the strength of association. Variables having less than 0.05 p-value of in the multivariable considered as factors associated with stillbirths. Result: The prevalence of stillbirth was 8.7% (95%CI: 5.8, 12.4). Rural dwelling (AOR=2.86, 95%CI: 1.10 7.47), twin pregnancy (AOR=6.69, 95% CI: 1.77, 25.2), medical or obstetrical illnesses during pregnancy (AOR=5.54, 95%CI: 1.71 17.94) and complications during labor and delivery (AOR=4.96, 95%CI: 1.48 16.58) were factors associated with stillbirths. Conclusion: This study revealed that the magnitude of stillbirth was high in rural dwelling, twin pregnancy, medical or obstetrical illnesses during pregnancy, and complicated labor were associated with increased occurrences of stillbirth. This finding suggests that particular emphasis on rural dwellers and strengthening quality of Maternal in pregnancy and labor delivery service and proper following during labor attending process are mandatory to reduce stillbirth.
背景:死产率是各国妇幼保健质量的指标之一。然而,在埃塞俄比亚,大多数新生儿死亡和几乎所有死产都被少报。因此,本研究旨在确定研究地区产后母亲中死产的发生率及其相关因素。方法:对2016年3 - 5月在Felegehiwot综合专科医院(n=310)进行机构横断面研究。采用预先测试的结构化访谈者管理问卷和病历回顾来收集产后母亲的数据。数据输入Epi Info version 7,使用SPSS version 20进行分析。二值logistic回归模型拟合识别死胎;计算校正优势比(AOR)和95%可信区间(CI)来评估关联强度。在被认为与死产相关的多变量因素中,p值小于0.05的变量。结果:死产率为8.7% (95%CI: 5.8, 12.4)。农村居住(AOR=2.86, 95%CI: 1.10 7.47)、双胎妊娠(AOR=6.69, 95%CI: 1.77, 25.2)、妊娠期内科或产科疾病(AOR=5.54, 95%CI: 1.71 17.94)以及分娩和分娩并发症(AOR=4.96, 95%CI: 1.48 16.58)是死产的相关因素。结论:本研究表明,农村地区死产发生率较高,双胎妊娠、妊娠期内科或产科疾病以及难产与死产发生率增加有关。这一发现表明,特别重视农村居民,加强孕产妇妊娠和分娩服务质量,并在分娩过程中进行适当的随访,是减少死产的必要条件。
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引用次数: 1
Ethical Principles Under the Challenge of Enhancing Medecine 医学进步挑战下的伦理原则
Pub Date : 2021-03-12 DOI: 10.11648/J.IJBECS.20210701.12
Ismaila Mboutngam
Our purpose in this article is to make a prospectivist evaluation of the enhancing medicine regarding the human nature. From this, we are interested by the future of humanity within the project of artificializing human life. In order to achieve our epistemological aim, we have distributed this work into three main parts. The first part is an analysis of medical ethical principles that are presented as the safety belt of human nature. The human being within Hippocratic tradition of medicine has always been treated with certain consideration since he is an absolute value. In all circumstances, physicians were bound to preserve live and protect human dignity. Then, the medical paradigm prevailing was the therapeutic one. In the second part, the concern is to scrutinize the biotechnological revolution mainly the process of genetic engineering. This revolution brought alongside medical practices another version of treating human being. It is the version of higher experimentation and scientific curiosity. Therefore, the practitioners of genetic engineering proceed by a profound intervention in human genome in order, not mainly to cure disease, but to discover what makes life and others human functions be possible. When these are discovered, they can program, design and enhance the future human being. This practice cannot go on without raising ethical questions such as the risk of alteration of human nature. It can also bring in the society the social injustice, giving the fact that those practices are more expensive to be at the level of all the social classes. The most eminent consequence of this social injustice is what we name bioimperialism where the natural human beings will become the slaves of artificial and enhanced human beings. Finally, we will bring a new perspective to contain the risks of enhancing medicine. It is necessary that man must recognize the limits of his power and the effects that the overuse of that power can generate as disasters. However, it is also relevant to notice that, enhancing medicine has already gained public opinion. Accordingly, theorical discourses of bioethicists and philosophical pessimism are not more able to bind the biomedical progress. What is important for bioethicists and humanity as whole, is a habitude of resilience consisting not of rejecting categorically the biomedical practices but to appreciate them according to the good there are able to achieve.
本文的目的是对增强人性的药物进行前瞻性评价。由此,我们感兴趣的是在人类生命的人工工程中人类的未来。为了达到我们认识论的目的,我们把本书分为三个主要部分。第一部分是对作为人性安全带的医学伦理原则的分析。在希波克拉底的医学传统中,人类总是被给予一定的考虑,因为他是一个绝对的价值。在任何情况下,医生都有义务维持生命和保护人的尊严。当时,流行的医学范式是治疗范式。第二部分关注的是审视生物技术革命,主要是基因工程的过程。这场革命带来了医疗实践的另一种治疗人类的方式。它是高等实验和科学好奇心的版本。因此,基因工程的实践者对人类基因组进行深刻的干预,主要不是为了治疗疾病,而是为了发现是什么使生命和其他人类功能成为可能。当这些被发现,他们可以编程,设计和提高未来的人类。这种做法不可能不引起诸如改变人性的风险等伦理问题而继续下去。它也会给社会带来社会不公正,使这些做法在所有社会阶层的层面上都更加昂贵。这种社会不公最显著的后果就是我们所说的生物帝国主义,自然的人类将成为人造的和增强的人类的奴隶。最后,我们将带来一个新的视角来遏制增强医学的风险。人类必须认识到自己力量的局限性,以及过度使用这种力量可能产生的灾难。然而,同样值得注意的是,强化医学已经获得了公众舆论。因此,生物伦理学家的理论话语和哲学悲观主义已不再能够束缚生物医学的进步。对生物伦理学家和整个人类来说,重要的是一种适应的习惯,不是断然拒绝生物医学实践,而是根据它们能够实现的好处来欣赏它们。
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引用次数: 0
Stroke in Sub-saharan Africa: Observations from Donka National Hospital 撒哈拉以南非洲的中风:来自东卡国家医院的观察
Pub Date : 2021-01-01 DOI: 10.11648/j.ijbecs.20210704.12
Vamala Guillavogui, D. Sylla, F. Sakadi, S. Diawara, Nestor Onikoyamou, Abdel-madjid Zakaria Zakaria, Kézély Béavogui, A. Cissé
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引用次数: 0
The Problem of Early Surgical Revisions After Abdominal Surgery, Study About 83 Cases Recorded in Bangui, Central African Republic 腹部手术后早期手术修复的问题——对中非共和国班吉83例病例的研究
Pub Date : 2021-01-01 DOI: 10.11648/j.ijbecs.20210704.13
Doui Doumgba Antoine, P. Germain, Damassara Kokonga Innocent, Ngboko Mirotiga Pétula Anicette
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引用次数: 0
Assessment of Maternal Outcome Among Preeclamptic Women at Dilla University Referral Hospital, Dilla Ethiopia 埃塞俄比亚迪拉大学转诊医院子痫前期妇女的产妇结局评估
Pub Date : 2021-01-01 DOI: 10.11648/j.ijbecs.20210703.14
Zerihun Figa, T. Temesgen, Etaferahu Belekle, Abas Ahimed, Ruth Tilahun
: Background: Pre-eclampsia is hypertension in pregnancy after 20weeks of gestation characterized blood pressure greater than 140/90 mm Hg, using the Korotkoff phase V sound for the diastolic value, on two occasions 4 hours apart. It is one of a spectrum of pregnancy disorders which result in different complications including maternal death. Methodology: Retrospective cross-sectional study design was employed. A total of 295 samples were recruited and systematic sampling technique was used to select study subjects who were admitted with preeclampsia from January1, 2016 and December 31, 2018 at Dilla University Referral Hospital. Medical records review was done using pretested data abstraction tool. Data was entered in EpiData version 4.4.2.1and exported into SPSS (statistical package of social science) version 25.0 for analysis. Binary and multiple logistic regressions were used to identify association between variables. Adjusted odds ratio along with 95% confidence interval was estimated to assess the strength of the association, and a p-value ≤ 0.05 was used to declare the level of statistical significance. Results: In this study 295 medical charts of pre-eclamptic women were reviewed. The most 210 (72.2%) of the participants were between the age of 20-34years. Severe type of preeclampsia was 174 (58.0%). HELLP syndrome was the most common complication of severe preeclampsia 81 (66.6%) followed by DIC, renal failure and liver failure, 25 (20.5%), 9 (7.4%) and 1 (0.8%) respectively. Maternal deaths due to preeclampsia were 6 this gives case fatality of 2%. In multivariable logistic regression, rural residence has 5.038 times more risk of unfavorable maternal outcome [AOR=5.038, 95%CI 1.971-12.879], gestational age ≤33 weeks has 3.67 times higher risk of unfavorable maternal outcome [AOR=3.67, 95%CI 1.829-7.364] and admission of women with diagnosis of sever preeclampsia has 6.42 times higher risk of unfavorable maternal outcome [AOR=6.42, 95%CI 2.017-21.103]. Conclusion and recommendation: Although there was current envisaged on maternal health improvement, this study has shown that maternal complications were common among pre-eclamptic women. The most common maternal complications due to preeclampsia were HELLP syndrome, DIC and renal failure. Health care professionals specially who work at PHC center should take appropriate trainings on immediate management and counseling a women coming for ANC and prompt referral for preeclampsia women with severity sign.
背景:先兆子痫是妊娠20周后的高血压,以血压大于140/90 mm Hg为特征,使用Korotkoff V相声压测定舒张值,两次间隔4小时。它是一系列妊娠疾病中的一种,可导致包括产妇死亡在内的各种并发症。方法:采用回顾性横断面研究设计。本研究共招募295例样本,采用系统抽样技术选择2016年1月1日至2018年12月31日在迪拉大学转诊医院就诊的先兆子痫患者。使用预先测试的数据抽象工具进行病历审查。数据输入EpiData 4.4.2.1版本,导出到SPSS 25.0版本进行分析。使用二元和多元逻辑回归来确定变量之间的关联。估计校正优势比和95%置信区间来评估相关性的强度,用p值≤0.05来表示具有统计学显著性的水平。结果:本研究回顾了295例子痫前期妇女的病历。其中年龄在20-34岁之间的人数最多,为210人(72.2%)。重度子痫前期174例(58.0%)。重度子痫前期最常见的并发症为HELLP综合征81例(66.6%),其次为DIC 25例(20.5%),肾功能衰竭9例(7.4%),肝功能衰竭1例(0.8%)。产妇因子痫前期死亡6例,病死率为2%。多变量logistic回归结果显示,农村居民发生不良结局的风险是农村居民的5.038倍[AOR=5.038, 95%CI 1.971 ~ 12.879],胎龄≤33周发生不良结局的风险是农村居民的3.67倍[AOR=3.67, 95%CI 1.829 ~ 7.364],诊断为重度先兆子痫的住院妇女发生不良结局的风险是农村居民的6.42倍[AOR=6.42, 95%CI 2.017 ~ 21.103]。结论和建议:虽然目前有改善产妇健康的设想,但这项研究表明,产妇并发症在子痫前期妇女中很常见。子痫前期最常见的产妇并发症是HELLP综合征、DIC和肾衰竭。特别是在初级保健中心工作的卫生保健专业人员应该接受适当的培训,以便对前来ANC的妇女进行即时管理和咨询,并及时转诊有严重症状的先兆子痫妇女。
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引用次数: 0
Using Eye Tracking to Explore Visual Attention in Adolescents With Autism Spectrum Disorder 用眼动追踪研究自闭症谱系障碍青少年的视觉注意
Pub Date : 2021-01-01 DOI: 10.4018/IJBCE.2021010101
Anne M. P. Michalek, Jonna Bobzien, Victor A Lugo, Chung-Hao Chen, A. Bruhn, M. Giannakos, Anne M. P. Michalek
Video social stories are used to facilitate understanding of social situations for adolescents with autism spectrum disorder (ASD). This study explored the use of eye tracking technology to understand how adolescents with and without ASD visually attend to video social story content and whether visual attention is related to content comprehension. Six adolescents, with and without ASD, viewed a video social story of visiting a dental office. Eye gaze metrics, including fixation duration and count, and visit duration were collected to measure visual attention, and a knowledge assessment was administered for comprehension. Results indicated adolescents with ASD fixated and maintained visual attention at rates lower than peers without ASD. Adolescents with ASD scored higher (M=77.78) than peers without ASD (M=72.22) on the assessment indicating no relationship between eye gaze metrics and knowledge accuracy. Impact and implications of visual image type on frequency and duration of visual attention generated by participants is discussed.
视频社交故事用于促进自闭症谱系障碍(ASD)青少年对社交情境的理解。本研究探讨了使用眼动追踪技术来了解有和没有ASD的青少年如何在视觉上注意视频社会故事内容,以及视觉注意是否与内容理解有关。六个有或没有自闭症谱系障碍的青少年观看了一段看牙医诊所的社交故事视频。收集眼球注视指标,包括注视时间和次数,以及访问持续时间来测量视觉注意力,并进行知识评估以了解理解情况。结果表明,与非自闭症青少年相比,自闭症青少年的视觉注意力固定和维持率较低。有ASD的青少年得分(M=77.78)高于无ASD的同龄人(M=72.22),表明注视指标与知识准确性之间没有关系。讨论了视觉图像类型对参与者产生视觉注意的频率和持续时间的影响和含义。
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引用次数: 0
Subtle Activities of Specific Plain Subtalar Joint May Account for Non-injured Ankle Pain: A Case Report 特定平距下关节的细微活动可能解释了非损伤性踝关节疼痛:一例报告
Pub Date : 2021-01-01 DOI: 10.11648/j.ijbecs.20210704.15
Shenxing Du, Lihong Wei
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引用次数: 0
Therapeutic Efficacy of Albendazole and Tetraclozan Against Gastrointestinal Worms in Crossbred Cows of HARC, Welmera District, Central Ethiopia 阿苯达唑和四环素对埃塞俄比亚中部韦尔梅拉地区HARC杂交奶牛胃肠道蠕虫的治疗效果
Pub Date : 2021-01-01 DOI: 10.11648/j.ijbecs.20210704.14
Beksisa Urge, Tamirat Seyoum, Temesgen Kassa, Markos Tadele, F. Gutema, Neima Arebu, U. Galmessa, Million Tadese
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引用次数: 0
期刊
International journal of biomedical engineering and clinical science
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