Pub Date : 2024-01-01Epub Date: 2024-07-23DOI: 10.26502/ogr0159
Jay S Mishra, Hanjie Zhao, Jing Zheng, Sathish Kumar
Background: Preeclampsia (PE) is a hypertensive disorder of pregnancy associated with adverse maternal and fetal outcomes. While placental dysfunction is implicated in PE pathogenesis, the impact of PE on placental lipid metabolism and its potential sexual dimorphism remains poorly understood.
Methods: We conducted a comprehensive analysis of term placentas from PE and normotensive pregnancies with male and female fetuses. Lipid profiles were quantified using mass spectrometry, and mRNA expression of genes involved in fatty acid oxidation, esterification, and transport was assessed using qPCR.
Results: Placentas from PE pregnancies exhibited elevated lipid levels, with male placentas showing a more pronounced increase in triacylglycerols, cholesteryl esters, and free cholesterol compared to female placentas. Gene expression analysis revealed sexually dimorphic alterations, with male PE placentas exhibiting upregulation of genes involved in fatty acid uptake, oxidation, and esterification, while female PE placentas showed a more complex response with both upregulation and downregulation of certain genes. Notably, peroxisomal fatty acid oxidation was upregulated in male PE placentas but suppressed in female PE placentas.
Conclusions: Our findings reveal sexually dimorphic alterations in placental lipid metabolism in PE, suggesting that male placentas may be more vulnerable to lipotoxicity. These insights may have implications for understanding the pathogenesis of PE and developing sex-specific interventions to improve maternal and fetal outcomes.
背景：子痫前期（PE）是一种妊娠期高血压疾病，与孕产妇和胎儿的不良结局有关。虽然胎盘功能障碍与子痫前期的发病机制有关，但人们对子痫前期对胎盘脂质代谢的影响及其潜在的性别二形性仍知之甚少：方法：我们对PE妊娠和血压正常妊娠的足月胎盘进行了全面分析。结果：PE 妊娠的胎盘表现出较高的性激素水平，而正常妊娠的胎盘则表现出较低的性激素水平：结果：PE妊娠胎盘中的脂质水平升高，与女性胎盘相比，男性胎盘中的三酰甘油、胆固醇酯和游离胆固醇的增加更为明显。基因表达分析显示了性别上的二态性改变，雄性 PE 胎盘中参与脂肪酸摄取、氧化和酯化的基因上调，而雌性 PE 胎盘则表现出更复杂的反应，某些基因既上调又下调。值得注意的是，男性PE胎盘中过氧体脂肪酸氧化基因上调，而女性PE胎盘中则受到抑制：我们的研究结果揭示了PE胎盘脂质代谢的性别双态性改变，表明男性胎盘可能更容易受到脂毒性的影响。这些发现可能有助于了解 PE 的发病机制，并制定针对不同性别的干预措施，以改善孕产妇和胎儿的预后。
{"title":"Sex-Specific Dysregulation of Placental Lipid Metabolism in Preeclampsia.","authors":"Jay S Mishra, Hanjie Zhao, Jing Zheng, Sathish Kumar","doi":"10.26502/ogr0159","DOIUrl":"10.26502/ogr0159","url":null,"abstract":"<p><strong>Background: </strong>Preeclampsia (PE) is a hypertensive disorder of pregnancy associated with adverse maternal and fetal outcomes. While placental dysfunction is implicated in PE pathogenesis, the impact of PE on placental lipid metabolism and its potential sexual dimorphism remains poorly understood.</p><p><strong>Methods: </strong>We conducted a comprehensive analysis of term placentas from PE and normotensive pregnancies with male and female fetuses. Lipid profiles were quantified using mass spectrometry, and mRNA expression of genes involved in fatty acid oxidation, esterification, and transport was assessed using qPCR.</p><p><strong>Results: </strong>Placentas from PE pregnancies exhibited elevated lipid levels, with male placentas showing a more pronounced increase in triacylglycerols, cholesteryl esters, and free cholesterol compared to female placentas. Gene expression analysis revealed sexually dimorphic alterations, with male PE placentas exhibiting upregulation of genes involved in fatty acid uptake, oxidation, and esterification, while female PE placentas showed a more complex response with both upregulation and downregulation of certain genes. Notably, peroxisomal fatty acid oxidation was upregulated in male PE placentas but suppressed in female PE placentas.</p><p><strong>Conclusions: </strong>Our findings reveal sexually dimorphic alterations in placental lipid metabolism in PE, suggesting that male placentas may be more vulnerable to lipotoxicity. These insights may have implications for understanding the pathogenesis of PE and developing sex-specific interventions to improve maternal and fetal outcomes.</p>","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11315440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141918275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Macrophages play a key role in all environmental conditions surrounding pregnancy. Coating of autologous red blood cells (RBCs) with polyclonal antibodies to Rh(D) antigen may result in an immunomodulation and improved outcome in Rh(D) positive women with recurrent pregnancy loss (RPL).
Methods: A total of 60 Rh(D) positive women (age 23 to 45 years) with a history of RPL and ineffective treatment with low molecular weight heparin (LMWH) and/or aspirin were included in this retrospective study. In addition to this treatment, Anti-D (300 μg) was given subcutaneously to each woman either prior to pregnancy and/or two times within 12 weeks of gestation.
Results: Treatment with Anti-D in non-responders to heparin/aspirin resulted in successful pregnancies in 67% of all cases. The remaining women had only aborts (23%) or did not become pregnant (10%). None of the treated women has developed anemia due to this treatment or any other significant adverse reaction. The rate of successful pregnancies does not appear to be influenced by the administration of: Anti-D prior to pregnancy, age, thrombophilia or previous alive births.
Conclusion: The improved outcome following the administration of Anti-D in women with RPL might be explained by immune modulations induced by different immune reactions including polarization of decidual macrophages. The results obtained in this study clearly indicate that Anti-D is safe and highly effective in treatment of Rh(D) positive women with RPL. However, further studies are required to support our results and to find out the optimal dose and timing of Anti-D administration.
背景:巨噬细胞在妊娠周围的所有环境条件中起着关键作用。Rh(D)抗原多克隆抗体包衣自体红细胞(rbc)可能导致Rh(D)阳性复发性妊娠丢失(RPL)妇女的免疫调节和改善结局。方法:回顾性研究60例Rh(D)阳性女性，年龄23 ~ 45岁，有RPL病史，低分子肝素(LMWH)和/或阿司匹林治疗无效。在此基础上，每个妇女在怀孕前皮下注射抗- d (300 μg)，并在妊娠12周内皮下注射两次。结果:在对肝素/阿司匹林无反应的患者中，抗- d治疗导致67%的患者成功怀孕。剩下的妇女只有流产(23%)或没有怀孕(10%)。所有接受治疗的妇女均未因这种治疗或任何其他重大不良反应而出现贫血。妊娠成功率似乎不受妊娠前服用抗- d、年龄、血栓形成或以前活产的影响。结论:抗- d对RPL患者预后的改善可能与不同免疫反应引起的免疫调节有关，包括巨噬细胞的极化。本研究结果清楚地表明Anti-D治疗Rh(D)阳性女性RPL安全有效。然而，需要进一步的研究来支持我们的结果，并找出抗- d给药的最佳剂量和时间。
{"title":"Polyclonal Anti-D Antibodies Significantly Reduce the Rate of Miscarriages in Rh(D) positive Women with Recurrent Pregnancy loss.","authors":"Frauke Ringel, Falk Lewandofski, Holger Kiesewetter, Jalid Sehouli, Berthold Hoppe, Alina Kiesewetter, Reinhard Hannen, Christian Friedrich Stoll, Sylvia Maas, Abdulgabar Salama","doi":"10.26502/ogr0119","DOIUrl":"https://doi.org/10.26502/ogr0119","url":null,"abstract":"<p><strong>Background: </strong>Macrophages play a key role in all environmental conditions surrounding pregnancy. Coating of autologous red blood cells (RBCs) with polyclonal antibodies to Rh(D) antigen may result in an immunomodulation and improved outcome in Rh(D) positive women with recurrent pregnancy loss (RPL).</p><p><strong>Methods: </strong>A total of 60 Rh(D) positive women (age 23 to 45 years) with a history of RPL and ineffective treatment with low molecular weight heparin (LMWH) and/or aspirin were included in this retrospective study. In addition to this treatment, Anti-D (300 μg) was given subcutaneously to each woman either prior to pregnancy and/or two times within 12 weeks of gestation.</p><p><strong>Results: </strong>Treatment with Anti-D in non-responders to heparin/aspirin resulted in successful pregnancies in 67% of all cases. The remaining women had only aborts (23%) or did not become pregnant (10%). None of the treated women has developed anemia due to this treatment or any other significant adverse reaction. The rate of successful pregnancies does not appear to be influenced by the administration of: Anti-D prior to pregnancy, age, thrombophilia or previous alive births.</p><p><strong>Conclusion: </strong>The improved outcome following the administration of Anti-D in women with RPL might be explained by immune modulations induced by different immune reactions including polarization of decidual macrophages. The results obtained in this study clearly indicate that Anti-D is safe and highly effective in treatment of Rh(D) positive women with RPL. However, further studies are required to support our results and to find out the optimal dose and timing of Anti-D administration.</p>","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10167743/pdf/nihms-1890894.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9522110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nehal Machado, A. Vasudeva, Sunanda Bharatnur, S. Kanchan
{"title":"Diagnostic and management dilemmas in secondary abdominal pregnancy: A case report and literature review of 314 cases from 1930-2021","authors":"Nehal Machado, A. Vasudeva, Sunanda Bharatnur, S. Kanchan","doi":"10.26502/ogr0111","DOIUrl":"https://doi.org/10.26502/ogr0111","url":null,"abstract":"","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Widest Uterine Grip Width Measurement Clinically to Predict the Success of the Vaginal Hysterectomy of a Very Large Fibroid Uterus","authors":"Ramkrishna Purohit, J. Sharma, Devajani Meher","doi":"10.26502/ogr0121","DOIUrl":"https://doi.org/10.26502/ogr0121","url":null,"abstract":"","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rafia Jeddy, Ahmed S. Keshta, Kamel Ikbariah, Mohamed S. Keshta
{"title":"A Literature Review Comparing Combined Oral Contraceptive Pill use and Mirena during Perimenopause","authors":"Rafia Jeddy, Ahmed S. Keshta, Kamel Ikbariah, Mohamed S. Keshta","doi":"10.26502/ogr0132","DOIUrl":"https://doi.org/10.26502/ogr0132","url":null,"abstract":"","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Karine Morcel, P. Merviel, P. James, S. Bouée, Mathilde Le Guillou, D. Pertuisel, J. Chabaud, S. Roche, Aurore Perrin, H. Drapier, D. Beauvillard
Early pregnancy loss (EPL) is a spontaneous miscarriage of a clinical pregnancy during the �rst trimester. Several factors of EPL have been studied but results were discordant. We performed a retrospective study in our ART center, comparing baseline data and IVF/ICSI outcomes between cycles with EPL, ongoing pregnancy and without pregnancy. Ectopic pregnancies and biochemical pregnancies (without visualization of a gestational sac on ultrasound) were excluded. The aim of this study is to compare these different cycles, and analyze the risk factors for EPL. We included 2555 IVF/ICSI cycles leading to 2193 oocyte pick-ups and 1806 embryo transfers. Several characteristics (women’s age, infertility diagnosis and duration, estradiol level on the day of hCG-trigger, endometrial thickness, day of embryo transfer) appeared to be risk factors of EPL in univariate analysis. Only women’s age has a signi�cant (p < 0.001) in�uence in multivariate analysis on the rate of EPL, with an OR: 1.71 if the woman’s age ≥ 35 years old (reference < 35 y.o = 1), 2.96 if ≥ 38 y.o and 5.31 if ≥ 40 y.o. In this study, we observed an increase in EPL rate by 4.15% per year in women over 35 years of age.
{"title":"Predictive Factors of Early Pregnancy Loss During In Vitro Fertilization/ Intracytoplasmic Sperm Injection (Ivf/Icsi): Retrospective Study on 1806 Embryo Transfers","authors":"Karine Morcel, P. Merviel, P. James, S. Bouée, Mathilde Le Guillou, D. Pertuisel, J. Chabaud, S. Roche, Aurore Perrin, H. Drapier, D. Beauvillard","doi":"10.26502/ogr0137","DOIUrl":"https://doi.org/10.26502/ogr0137","url":null,"abstract":"Early pregnancy loss (EPL) is a spontaneous miscarriage of a clinical pregnancy during the �rst trimester. Several factors of EPL have been studied but results were discordant. We performed a retrospective study in our ART center, comparing baseline data and IVF/ICSI outcomes between cycles with EPL, ongoing pregnancy and without pregnancy. Ectopic pregnancies and biochemical pregnancies (without visualization of a gestational sac on ultrasound) were excluded. The aim of this study is to compare these different cycles, and analyze the risk factors for EPL. We included 2555 IVF/ICSI cycles leading to 2193 oocyte pick-ups and 1806 embryo transfers. Several characteristics (women’s age, infertility diagnosis and duration, estradiol level on the day of hCG-trigger, endometrial thickness, day of embryo transfer) appeared to be risk factors of EPL in univariate analysis. Only women’s age has a signi�cant (p < 0.001) in�uence in multivariate analysis on the rate of EPL, with an OR: 1.71 if the woman’s age ≥ 35 years old (reference < 35 y.o = 1), 2.96 if ≥ 38 y.o and 5.31 if ≥ 40 y.o. In this study, we observed an increase in EPL rate by 4.15% per year in women over 35 years of age.","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Monterrosa-Castro A, F. J, Romero-Martínez G, Romero-Martínez S
{"title":"Association between Probable Generalized Anxiety Disorder with The Planning or Acceptance of The Pregnancy From The Beginning in Women Undergoing Prenatal Care","authors":"Monterrosa-Castro A, F. J, Romero-Martínez G, Romero-Martínez S","doi":"10.26502/ogr0115","DOIUrl":"https://doi.org/10.26502/ogr0115","url":null,"abstract":"","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Nhampossa, K. Munguambe, Célia Chaúque, Mariza Chivangue, Maura Mazuze, Anete Mendes, L. García-Otero, R. González, Esperança Sevene, C. Menéndez, N. Torres
{"title":"Knowledge of Pregnant Women in Rural Mozambique on Routine Practices to Prevent and Treat Common Conditions at the Antenatal Care Clinic","authors":"T. Nhampossa, K. Munguambe, Célia Chaúque, Mariza Chivangue, Maura Mazuze, Anete Mendes, L. García-Otero, R. González, Esperança Sevene, C. Menéndez, N. Torres","doi":"10.26502/ogr0117","DOIUrl":"https://doi.org/10.26502/ogr0117","url":null,"abstract":"","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Garuti, P. Sagrada, M. Mirra, Serena Migliaccio, A. Finco, M. Soligo
{"title":"Hysteroscopic Imaging of Health Endometrium in Premenopausal Breast Cancer Patients Taking Anti-estrogen Therapies","authors":"G. Garuti, P. Sagrada, M. Mirra, Serena Migliaccio, A. Finco, M. Soligo","doi":"10.26502/ogr0131","DOIUrl":"https://doi.org/10.26502/ogr0131","url":null,"abstract":"","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69352569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wang Yue, Wu jiaxing, Malin Di, S. Xin, Lin Lin, Wang Bo, Huang Mingli
{"title":"Effect of cervical pessary on pregnancy outcome in patients with twin pregnancies: a systematic review and meta-analysis","authors":"Wang Yue, Wu jiaxing, Malin Di, S. Xin, Lin Lin, Wang Bo, Huang Mingli","doi":"10.26502/ogr0110","DOIUrl":"https://doi.org/10.26502/ogr0110","url":null,"abstract":"","PeriodicalId":74336,"journal":{"name":"Obstetrics and gynecology research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69351999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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