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Role of Fungi in Bioremediation 真菌在生物修复中的作用
Pub Date : 2019-01-25 DOI: 10.19080/AIBM.2019.12.555841
Hussein Al-Nasrawi
Many chemical compounds in nature which discharged by human activities like oil spills and hydrocarbons, contain a toxic metal which consider a hazardous material against environment [1]. The nature is a huge ecological niche to fungi and bacteria which play an important role as decomposers of dead materials and converts organic matters into carbon dioxide and mineral molecules. Microorganisms have evolved a high degree of metabolic versatility that allows them to use a diverse range of organic substrates and large molecules from different complex chemicals present in nature such as hydrocarbons via activity of fungi which needs carbon source for their metabolic pathways in glycolysis process to produce primary and secondary metabolites.
人类活动在自然界中排放的许多化合物,如石油泄漏和碳氢化合物,都含有一种有毒金属,被认为是对环境有害的物质[1]。自然界对真菌和细菌来说是一个巨大的生态位,它们是死亡物质的分解者,将有机物转化为二氧化碳和矿物分子。微生物已经进化出高度的代谢多功能性,使它们能够利用自然界中存在的不同复杂化学物质(如碳氢化合物)的各种有机底物和大分子,通过真菌的活动,在糖酵解过程中其代谢途径需要碳源来产生初级和次级代谢物。
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引用次数: 0
Bacterial Cold Shock Proteins - the Molecular Chaperones for Multiple Stress Tolerance 细菌冷休克蛋白-多重应激耐受的分子伴侣
Pub Date : 2019-01-22 DOI: 10.19080/AIBM.2019.12.555837
K. Pb
Bacteria encounter different changing environments but resist the environmental changes by developing an array of mechanisms which protect them from adverse conditions. During cold conditions, fluidity of cell membranes decreases, and this results in lowering of active transport and protein secretion [1]. Further, all the molecular mechanisms gets impaired due to stabilization of secondary structures of DNA, RNA, and proteins [2]. RNA binding proteins (RNA chaperones) are ubiquitous and found in all living organisms and help to resolve the misfolded RNA structures under abiotic stress conditions. During rapid drop in temperatures, cold-induced proteins (Cips) are produced to protect the cells. With an increase in the cold conditions, the production of Cips also increases [3]. Different types of Cips like cold shock protein (Csp) family, RNA helicase csdA, exoribonucleases, PNPase and RNaseR, initiation factors 2a and 2b, NusA, and RecA [4-7] have been identified in Escherichia coli (E. coli). The Csps are one of the major Cips produced under cold conditions mainly in bacteria [4,8].
细菌遇到不同的变化环境,但通过发展一系列保护它们免受不利条件影响的机制来抵抗环境变化。在寒冷条件下,细胞膜的流动性降低,导致主动运输和蛋白质分泌降低[1]。此外,由于DNA、RNA和蛋白质二级结构的稳定,所有的分子机制都受到损害[2]。RNA结合蛋白(RNA chaperone)普遍存在于所有生物体中,并有助于解决非生物胁迫条件下错误折叠的RNA结构。在温度迅速下降的过程中,产生冷诱导蛋白(Cips)来保护细胞。随着寒冷条件的增加,cip的产量也随之增加[3]。在大肠杆菌中发现了不同类型的cip,如冷休克蛋白(Csp)家族、RNA解旋酶csdA、外核糖核酸酶、PNPase和RNaseR、起始因子2a和2b、NusA和RecA[4-7]。Csps是低温条件下主要在细菌中产生的主要cip之一[4,8]。
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引用次数: 9
Physical Properties and Biodegradable Study of Metalized and Non-Metalized Polypropylene (PP) Films: A Comparative Research 金属化与非金属化聚丙烯(PP)薄膜的物理性能与生物降解研究:比较研究
Pub Date : 2019-01-22 DOI: 10.19080/AIBM.2019.12.555838
Q ShaguftaIshteya
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引用次数: 2
Microplastic Pollution: An Overview of Current Scenario, Challenges, and Research Gaps 微塑料污染:现状、挑战和研究差距概述
Pub Date : 2019-01-22 DOI: 10.19080/AIBM.2019.12.555836
S. Khare
Globally presence of Microplastics (MPs) in the environment is identified as one of the major environmental threat. MPs are small plastic fragments (0.001-5 mm) and are ubiquitous in the environment especially in the marine and freshwater ecosystems. Majorly, they originate as a degradation products of larger plastic materials, or directly originate from personnel care products and synthetic fabrics. Smaller size of MPs results in their ingestion by a wide range of aquatic organisms ranging from zooplankton to fish, implying the potential for microplastics to accumulate in the marine food web. In this way, microplastics can potentially impact food safety and human health also. Recently presence of MPs in drinking water and table salt has sought the attention of worldwide researchers. In addition to that, hydrophobic nature of MPs also makes them as an active site for sorption of number of toxic persistent organic and inorganic contaminants and this too imparts toxicity to them. However, still our understanding with respect to ecological impact of MPs on the terrestrial environment is limited. There is also a need for comprehensive evaluation and assessment of MPs in different environmental systems in order to obtain a complete scenario of the extent of pollution. Toxicity evaluation of the same also needs to be assessed depthly. In the present review a brief overview of microplastic pollution, its toxicity assessment along with major challenges and mitigation options are discussed.
全球环境中存在的微塑料(MPs)被确定为主要的环境威胁之一。MPs是小的塑料碎片(0.001-5毫米),在环境中普遍存在,特别是在海洋和淡水生态系统中。它们主要来自较大塑料材料的降解产物,或直接来自人员护理产品和合成织物。小尺寸的微塑料会被从浮游动物到鱼类等各种水生生物摄入,这意味着微塑料有可能在海洋食物网中积累。通过这种方式,微塑料也可能影响食品安全和人类健康。最近,饮用水和食盐中存在的MPs引起了全世界研究人员的注意。此外,MPs的疏水性也使其成为吸附许多有毒持久性有机和无机污染物的活性位点,这也赋予了它们毒性。然而,我们对MPs对陆地环境的生态影响的了解仍然有限。还需要对不同环境系统中的MPs进行综合评价和评估,以便获得污染程度的完整情景。同样的毒性评价也需要深入评估。在本综述中,简要概述了微塑料污染,其毒性评估以及主要挑战和缓解方案。
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引用次数: 4
An in-Silico Approach to Understanding the Structure-Function: A Molecular Dynamics Simulation Study of Rand Serine Protease Properties from Bacillus Subtilis in Aqueous Solvents 一种理解结构-功能的计算机方法:水溶液中枯草芽孢杆菌兰德丝氨酸蛋白酶性质的分子动力学模拟研究
Pub Date : 2019-01-11 DOI: 10.19080/aibm.2019.12.555834
R. Rahman
Serine proteases from the Bacillus species extensively applied in the biotechnological application [1,2] such as detergent, leather and food industries, frequently under non-physiological conditions. New proteases with improved performance at extreme temperatures and in the presence of chemical additives may have great economic potential. The increasing availability of genetic sequences from completely different environments makes homology-based screening an attractive strategy for the discovery of new proteases [3]. So far, the broad investigation on proteases gave the basic understanding of their catalytic mechanism and their structure-function. Computational structure analysis and homology modeling can be a key process for the 3D structure reconstruction which facilitates the protein-protein interaction research. Protein crystal is the basic necessity to obtain the 3D structure [4].
来自芽孢杆菌种类的丝氨酸蛋白酶广泛应用于生物技术应用[1,2],如洗涤剂,皮革和食品工业,经常在非生理条件下。在极端温度和化学添加剂的存在下,具有改进性能的新型蛋白酶可能具有巨大的经济潜力。来自完全不同环境的基因序列越来越多,使得基于同源性的筛选成为发现新蛋白酶的一种有吸引力的策略[3]。迄今为止,对蛋白酶的广泛研究使人们对蛋白酶的催化机理和结构功能有了基本的认识。计算结构分析和同源性建模是三维结构重建的关键步骤,有助于蛋白质相互作用的研究。蛋白质晶体是获得蛋白质三维结构的基本条件[4]。
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引用次数: 1
Degradation of Phenol, an Innovative Biological Approach 苯酚降解,一种创新的生物方法
Pub Date : 2019-01-11 DOI: 10.19080/aibm.2019.12.555835
A. R. Choudhury
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引用次数: 2
A New Osmotolerant Plant Growth Promoting Bacillus Sp1 and Sp2 with Potential Antifungal Activity and Their Utilization of Ulva Lactuca as Natural Osmoprotectant 一种具有潜在抗真菌活性的促渗透芽孢杆菌Sp1和Sp2及其作为天然渗透保护剂的利用
Pub Date : 2019-01-11 DOI: 10.19080/aibm.2019.12.555832
E. Nabti
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引用次数: 0
Allele-specific genomic variations and transcriptomic research on quantitative phenotyping in plants 植物等位基因特异性基因组变异及定量表型的转录组学研究
Pub Date : 2019-01-11 DOI: 10.19080/AIBM.2019.12.555833
Kan Liu
Genomes and interactions among gene products with other molecules are the physical fundamentals of biological systems. This is especially true for research in plants, which usually have complicated genomes and many important traits like yield, plant height, and stress tolerance are quantitative. Many quantitative traits are usually controlled by more than one Quantitative expression Loci (QTL) through the regulation of gene expression. The expression of a gene could be associated with a genetic variant far away from it, which is called trans-eQTL (expression QTL) or be affected by a local variant, which is named cis-eQTL. With a widespread existence throughout the plant genome, cis-acting genetic variants have been proven to account for a larger proportion of variation in gene expression. However, it is challenging for identifying cis-eQTL in the population with sequencing data because the power of eQTL mapping is either constrained by sample size or reduced by confounding factors. For organisms with a diploid genome, the information of AlleleSpecific Expression (ASE) which could provide more direct evidence of cis-eQTL is often ignored or discarded due to the unavailability of haplotype information and mapping bias.
基因组和基因产物与其他分子之间的相互作用是生物系统的物理基础。对植物的研究尤其如此,因为植物通常具有复杂的基因组,而且许多重要的性状,如产量、株高和抗逆性都是定量的。许多数量性状通常通过基因表达调控而受多个数量表达位点(QTL)控制。一个基因的表达可能与一个远离它的遗传变异有关,这被称为反式eqtl(表达QTL),也可能受到一个局部变异的影响,这被称为顺式eqtl。由于在植物基因组中广泛存在,顺式作用的遗传变异已被证明在基因表达变异中占更大的比例。然而,利用测序数据在人群中识别顺式eQTL是具有挑战性的,因为eQTL定位的能力要么受到样本量的限制,要么受到混杂因素的影响。对于具有二倍体基因组的生物体,由于单倍型信息的缺乏和定位偏差,可以提供更直接的顺式eqtl证据的等位基因特异性表达(allelspecific Expression, ASE)信息往往被忽略或丢弃。
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引用次数: 0
Biotransformation of Selenite to Red Elemental Selenium 亚硒酸盐向红色元素硒的生物转化
Pub Date : 2019-01-03 DOI: 10.19080/AIBM.2019.12.555830
S. Raza
Selenium is an element of great interest due to its nutritional importance for humans and animals. Selenium undergoes biotic transformation like oxidation, dissimulator and assimilatory reduction, alkylation and de-alkylation, also due to which “cocktail” of various selenium specie is present in environment, posing a big challenge to Se specie analysis. In current study, we isolated bacterial strain, Bacillus licheniformis, Exiguobacterium sp., Bacillus subtilis, that convert (SeO 3 ) -2 (toxic) to elemental (reduced and less toxic) selenium. Different physical parameters were checked experimentally to increase Se reduction like pH, temperature, selenite concentration, incubation time, aeration. Se reduction increased with increased pH, Maximum reduction, 31 to 90% was check at pH 9 and different strain have different ranges of temperature, optimum temperature 37 °C and 44 °C was noted for selenium reduction. Microbes can reduce selenium in aerobic as well as anaerobic condition and reduction decrease with the increase in incubation time.
硒是一种非常有趣的元素,因为它对人类和动物具有重要的营养意义。硒经历了氧化、脱模拟器和同化还原、烷基化和去烷基化等生物转化过程,环境中也存在多种硒的“鸡尾酒”,对硒的分析提出了很大的挑战。在目前的研究中,我们分离了一株细菌,地衣芽孢杆菌,出口细菌sp.,枯草芽孢杆菌,将(SeO 3) -2(有毒)转化为元素(还原和低毒性)硒。实验考察了pH、温度、亚硒酸盐浓度、孵育时间、曝气等物理参数对硒还原的影响。硒还原量随着pH的增加而增加,pH为9时最大还原量为31 ~ 90%,不同菌株的温度范围不同,硒还原的最佳温度为37°C和44°C。微生物在好氧和厌氧条件下均能对硒进行还原,且还原量随培养时间的增加而减小。
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引用次数: 0
Evaluation of serum proteins in relation with parasitic infections 评价血清蛋白与寄生虫感染的关系
Pub Date : 2019-01-03 DOI: 10.19080/AIBM.2019.12.555831
Mohammad Lateef Ganaie
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引用次数: 2
期刊
Advances in Biotechnology & Microbiology
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